Rh5AG431000

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000175
Physical Location & Seq
Forward (+)
74100524 .. 74104021
3498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG431000.1

Sequence Viewer

Length: 2100 bp
ATGGATACCCTTTACAGATATTCTGTCTCCAATCCCATGGAGAGGTTCAAATTTGACCATGGCCCAGTTTCAGTCTGTCCAAATCCCAATCTTGTAAATGTAAATGGGTTCAAAATAAATCATGAATCCACCAATCCAGTATTACTTGCATCCAAATCTAATCTTTCTAGTGATTCAAGTAATGAGGGTTCAGAGGTAGATGGTCTTGATATCAATGACTGCAACCATCCTGTACTCAAGTACATAAATGATATTCTTCTGGAAGAAGACTTGGAGGGTAAGCCCTGCATGTTGCAGGATTGTTTAGCCCTCCAAGCTGCTGAGAAGTCTTTCTATGATGTCCTTAATCAGAAGGACCCTCCTTTACCCGACCAACCCTCTAGTTCTGTGCACCAAAGCTCTGAGAGTTCAGATGATGGTTCCCCACAGAGTTTCCGAAACAGTAACGGCTCTTTTTCTGTTAAGACAGATTGGGTTTGGGATCCGGAATGCTCTCCCGTCCAGTCTTCTCTATTTGAGTCTTCGTCGAATACCCGTTTAGTTTCTGATTCATTGTCTGAGATGCAAAGTCTTGGGAATTTTGGAGGTGTGGAGGAAGCAAGCAAGTTCCTTTCAAATCCGAAACTTGAAATGTTTGACTTAGAGAGGTTTCAATTGATGCCTCAAGGTTCAGTCGCATCTGAGTCAGGGGCTGAAGGCTACAACTCGACCAATGGATCAAAGGGAAAGAAGACTCATCAAAGGGAGGATAGTCATTTTCCAGAAGAAGAGAGAAGCAACAAGCAGTCTGCTGCTGTAGGGGACGACTTTGAGCCACAAGAAATGTTTGATGAGGTTTTGCTTTGTCCTGAGGTTGAGCTCTGTGCTGATTATGAGTCTTTGAAAACTGAAGCAAATGAAAAGTTGCAGCGTAACAAGCAGTTAAAAGGATTGAAGGCAAAGCGACGGAGTAATATTGGTGAAGTGGTAGATTTGTGCACAATGCTAACTCAATGTGCACAAGCTGTGGCGAACTTTGACCAACGAACTGCAAGTGAACTACTCAAGCAGATAAGGGAGCACACAACCCCCCATGGTGATGCAACCCAGAGGTTAGCCCATTACTTTGCTGATGGCCTCCGGACGCGCTTGGCTGGTGCCAAAACCCCATCGTATTCACCTCTTGTAAGTGTGCAGACATCAGCTGCTGAAATCTTAAAAGCATACAAGGTATTTATTACAGCATGCCCTTTCAAGAAGATGTCACACTTCTTTGCCAACAGAACAATCATGAAGCTAGCTGAAAAAGCAACAAGGCTTCACATTATTGATTTTGGTATTTCTTATGGATTTCAATGGCCTTGCCTGATCCAACGTCTGTCCCAAAGACCTGGTGGACCTCCTAAGCTTCGTATCACAGCAATAGAGCTTCCCCAACCAGGTTTTCGACCTACAGAAAGAGTTGAAGAGACCGGACGTCGCTTAGAAAAATATGCCAAGAGATTTGATGTCCCGTTTGAGTACAATGTCATTGCCCAGAAATGGGAAACTATCCGAATTGAGGACATCAAAATTGACAGAACTGAGCTGATTGTGGTCAATTGTTTGCACCGGTTAAGGAACATACCAGATGAGACGGTGATGGTGAACAGTCCAAGAGATGCTGTACTTAAGTTGATAAGGAAAATTTGTCCAGACCTTTTCATTCATGGTGTTGTCAATGGGACATACAATGCTCCCTTCTTTCTCACACGATTTAGAGAGGCACTCCTCCACTTCTATTCACTATTTGATATGGTTGAGGTGACCATCTCTCGTGAAGATGAGCAGCGGATGTTGTTTGAACAAGCAATATATGGCAGAGATATAATTAATGTCGTAGCGTGTGAAGGACTGGAAAGAGTTGAAAGGCCTGAAACATACAGGCAGTGGCAGGTCAGGAATTTGAGGGCTGGGTTCAAGCAGCTACCATTAGACCAGAAGCTGTTGAAGGAAGTGAAGACTATGTCAAAAGTAATGCGTTATCATAATGATTTTAGAGTTGATGAAGATGGCCATTGGATGCTGCAGGGATGGAAAGGAAGAACCCTCATGGCTCTTTCTTTTTGGAAGCCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS RVT_2

Protein Analysis

699

Amino Acids

79.59

Weight (kDa)

5.91

Isoelectric Point (pI)

49.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 327 - 697 3.3e-122 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1459
Acc36I ACCTGC 1 cut(s) 1903
AccB1I GGYRCC 1 cut(s) 1136
AccII CGCG 1 cut(s) 1126
AccIII TCCGGA 2 cut(s) 484, 1119
AciI CCGC 1 cut(s) 1810
AclWI GGATC 4 cut(s) 476, 489, 724, 1342
AcoI YGGCCR 1 cut(s) 2032
AcsI RAATTY 4 cut(s) 50, 577, 1665, 1921
AcuI CTGAAG 2 cut(s) 714, 909
AcyI GRCGYC 1 cut(s) 1456
AfaI GTAC 4 cut(s) 234, 242, 1502, 1647
AfiI CCNNNNNNNGG 5 cut(s) 42, 1418, 1521, 1522, 1540
AflII CTTAAG 1 cut(s) 1649
AgeI ACCGGT 1 cut(s) 1590
AhdI GACNNNNNGTC 1 cut(s) 1455
AjnI CCWGG 2 cut(s) 1369, 1417
AjuI GAANNNNNNNTTGG 2 cut(s) 1407, 1439
Alw21I GWGCWC 5 cut(s) 393, 861, 980, 1000, 1062
Alw26I GTCTC 3 cut(s) 31, 1442, 1607
Alw44I GTGCAC 3 cut(s) 389, 976, 996
AlwI GGATC 4 cut(s) 476, 489, 724, 1342
AlwNI CAGNNNCTG 3 cut(s) 692, 1187, 1963
Aor13HI TCCGGA 2 cut(s) 484, 1119
AoxI GGCC 5 cut(s) 61, 1114, 1337, 1889, 2032
ApaLI GTGCAC 3 cut(s) 389, 976, 996
ApeKI GCWGC 7 cut(s) 317, 791, 907, 1184, 1807, 1942, 2044
ApoI RAATTY 4 cut(s) 50, 577, 1665, 1921
AseI ATTAAT 1 cut(s) 1851
AsiGI ACCGGT 1 cut(s) 1590
Asp700I GAANNNNTTC 1 cut(s) 329
AspLEI GCGC 1 cut(s) 1128
AspS9I GGNCC 3 cut(s) 62, 355, 1376
AsuHPI GGTGA 6 cut(s) 971, 1088, 1149, 1630, 1636, 1795
AsuNHI GCTAGC 1 cut(s) 1276
AvaII GGWCC 2 cut(s) 355, 1376
AxyI CCTNAGG 1 cut(s) 849
BaeGI GKGCMC 3 cut(s) 393, 980, 1000
BalI TGGCCA 1 cut(s) 2034
BamHI GGATCC 1 cut(s) 481
BanI GGYRCC 1 cut(s) 1136
BanII GRGCYC 1 cut(s) 861
BauI CACGAG 1 cut(s) 1794
BbsI GAAGAC 5 cut(s) 273, 498, 513, 737, 1985
Bbv12I GWGCWC 5 cut(s) 393, 861, 980, 1000, 1062
BbvI GCAGC 7 cut(s) 304, 778, 919, 1171, 1819, 1954, 2031
BccI CCATC 9 cut(s) 194, 234, 410, 1106, 1156, 1615, 1796, 2024, 2046
BceAI ACGGC 1 cut(s) 463
BciT130I CCWGG 2 cut(s) 1371, 1419
BcoDI GTCTC 3 cut(s) 31, 1442, 1607
BfaI CTAG 5 cut(s) 168, 381, 1277, 2094, 2098
BfmI CTRYAG 3 cut(s) 795, 1431, 2045
BfrI CTTAAG 1 cut(s) 1649
BfuAI ACCTGC 1 cut(s) 1903
BisI GCNGC 7 cut(s) 318, 792, 908, 1185, 1808, 1943, 2045
BlsI GCNGC 7 cut(s) 319, 793, 909, 1186, 1809, 1944, 2046
Bme1390I CCNGG 2 cut(s) 1371, 1419
Bme18I GGWCC 2 cut(s) 355, 1376
BmeRI GACNNNNNGTC 1 cut(s) 1455
BmgT120I GGNCC 3 cut(s) 62, 355, 1376
BmiI GGNNCC 4 cut(s) 357, 421, 483, 1138
BmrFI CCNGG 2 cut(s) 1371, 1419
BmrI ACTGGG 1 cut(s) 59
BmsI GCATC 7 cut(s) 158, 552, 648, 686, 1069, 1630, 2031
BmtI GCTAGC 1 cut(s) 1280
BmuI ACTGGG 1 cut(s) 59
BpiI GAAGAC 5 cut(s) 273, 498, 513, 737, 1985
BplI GAGNNNNNCTC 2 cut(s) 1731, 1763
Bpu10I CCTNAGC 1 cut(s) 1383
BpuEI CTTGAG 3 cut(s) 221, 648, 1028
BsaHI GRCGYC 1 cut(s) 1456
BsaI GGTCTC 1 cut(s) 1442
BsaJI CCNNGG 3 cut(s) 36, 58, 1072
BsaWI WCCGGW 4 cut(s) 484, 1119, 1451, 1590
Bsc4I CCNNNNNNNGG 5 cut(s) 42, 1418, 1521, 1522, 1540
Bse118I RCCGGY 1 cut(s) 1590
Bse1I ACTGG 4 cut(s) 65, 137, 502, 1878
Bse21I CCTNAGG 1 cut(s) 849
Bse3DI GCAATG 1 cut(s) 1509
BseAI TCCGGA 2 cut(s) 484, 1119
BseBI CCWGG 2 cut(s) 1371, 1419
BseDI CCNNGG 3 cut(s) 36, 58, 1072
BseGI GGATG 5 cut(s) 149, 226, 1818, 2046, 2057
BseLI CCNNNNNNNGG 5 cut(s) 42, 1418, 1521, 1522, 1540
BseMI GCAATG 1 cut(s) 1509
BseMII CTCAG 6 cut(s) 312, 393, 549, 672, 840, 1554
BseNI ACTGG 4 cut(s) 65, 137, 502, 1878
BseRI GAGGAG 1 cut(s) 1739
BseSI GKGCMC 3 cut(s) 393, 980, 1000
BseXI GCAGC 7 cut(s) 304, 778, 919, 1171, 1819, 1954, 2031
BseYI CCCAGC 1 cut(s) 1931
BsgI GTGCAG 1 cut(s) 1193
Bsh1236I CGCG 1 cut(s) 1126
BshFI GGCC 5 cut(s) 63, 1116, 1339, 1891, 2034
BshNI GGYRCC 1 cut(s) 1136
BshTI ACCGGT 1 cut(s) 1590
BsiHKAI GWGCWC 5 cut(s) 393, 861, 980, 1000, 1062
BsiSI CCGG 4 cut(s) 485, 1120, 1452, 1591
BslFI GGGAC 4 cut(s) 815, 1345, 1475, 1717
BslI CCNNNNNNNGG 5 cut(s) 42, 1418, 1521, 1522, 1540
BsmAI GTCTC 3 cut(s) 31, 1442, 1607
BsmBI CGTCTC 1 cut(s) 1607
BsmFI GGGAC 4 cut(s) 815, 1345, 1475, 1717
BsmI GAATGC 1 cut(s) 494
BsnI GGCC 5 cut(s) 63, 1116, 1339, 1891, 2034
Bso31I GGTCTC 1 cut(s) 1442
Bsp1286I GDGCHC 5 cut(s) 393, 861, 980, 1000, 1062
Bsp13I TCCGGA 2 cut(s) 484, 1119
Bsp143I GATC 3 cut(s) 481, 716, 1347
Bsp19I CCATGG 3 cut(s) 36, 58, 1072
BspACI CCGC 1 cut(s) 1810
BspANI GGCC 5 cut(s) 63, 1116, 1339, 1891, 2034
BspCNI CTCAG 6 cut(s) 313, 394, 550, 673, 841, 1555
BspEI TCCGGA 2 cut(s) 484, 1119
BspFNI CGCG 1 cut(s) 1126
BspHI TCATGA 2 cut(s) 121, 1269
BspLI GGNNCC 4 cut(s) 357, 421, 483, 1138
BspMAI CTGCAG 1 cut(s) 2049
BspMI ACCTGC 1 cut(s) 1903
BspOI GCTAGC 1 cut(s) 1280
BspPI GGATC 4 cut(s) 476, 489, 724, 1342
BspT107I GGYRCC 1 cut(s) 1136
BspTI CTTAAG 1 cut(s) 1649
BspTNI GGTCTC 1 cut(s) 1442
BsrDI GCAATG 1 cut(s) 1509
BsrFI RCCGGY 1 cut(s) 1590
BsrI ACTGG 4 cut(s) 65, 137, 502, 1878
BssAI RCCGGY 1 cut(s) 1590
BssECI CCNNGG 3 cut(s) 36, 58, 1072
BssMI GATC 3 cut(s) 481, 716, 1347
BssNI GRCGYC 1 cut(s) 1456
BssSI CACGAG 1 cut(s) 1794
BssT1I CCWWGG 3 cut(s) 36, 58, 1072
Bst2BI CACGAG 1 cut(s) 1794
Bst2UI CCWGG 2 cut(s) 1371, 1419
Bst4CI ACNGT 3 cut(s) 443, 1618, 1631
Bst6I CTCTTC 2 cut(s) 762, 1440
BstACI GRCGYC 1 cut(s) 1456
BstAFI CTTAAG 1 cut(s) 1649
BstC8I GCNNGC 3 cut(s) 601, 1225, 1278
BstDEI CTNAG 9 cut(s) 321, 402, 558, 640, 681, 849, 1383, 1462, 1563
BstDSI CCRYGG 3 cut(s) 36, 58, 1072
BstEII GGTNACC 1 cut(s) 1783
BstF5I GGATG 5 cut(s) 149, 226, 1818, 2046, 2057
BstFNI CGCG 1 cut(s) 1126
BstHHI GCGC 1 cut(s) 1128
BstKTI GATC 3 cut(s) 484, 719, 1350
BstMAI GTCTC 3 cut(s) 31, 1442, 1607
BstMBI GATC 3 cut(s) 481, 716, 1347
BstMWI GCNNNNNNNGC 3 cut(s) 314, 916, 1286
BstNI CCWGG 2 cut(s) 1371, 1419
BstNSI RCATGY 2 cut(s) 292, 1227
BstPI GGTNACC 1 cut(s) 1783
BstSCI CCNGG 2 cut(s) 1369, 1417
BstSFI CTRYAG 3 cut(s) 795, 1431, 2045
BstSLI GKGCMC 3 cut(s) 393, 980, 1000
BstUI CGCG 1 cut(s) 1126
BstV1I GCAGC 7 cut(s) 304, 778, 919, 1171, 1819, 1954, 2031
BstV2I GAAGAC 5 cut(s) 273, 498, 513, 737, 1985
BstX2I RGATCY 1 cut(s) 481
BstXI CCANNNNNNTGG 2 cut(s) 37, 1370
BstYI RGATCY 1 cut(s) 481
Bsu36I CCTNAGG 1 cut(s) 849
BsuRI GGCC 5 cut(s) 63, 1116, 1339, 1891, 2034
BtgI CCRYGG 3 cut(s) 36, 58, 1072
BtsCI GGATG 5 cut(s) 149, 226, 1818, 2046, 2057
BtsI GCAGTG 1 cut(s) 1913
BtsIMutI CAGTG 1 cut(s) 1913
BveI ACCTGC 1 cut(s) 1903
Cac8I GCNNGC 3 cut(s) 601, 1225, 1278
CaiI CAGNNNCTG 3 cut(s) 692, 1187, 1963
CciI TCATGA 2 cut(s) 121, 1269
CfoI GCGC 1 cut(s) 1128
Cfr10I RCCGGY 1 cut(s) 1590
Cfr13I GGNCC 3 cut(s) 62, 355, 1376
CseI GACGC 1 cut(s) 1132
CsiI ACCWGGT 2 cut(s) 1369, 1417
Csp6I GTAC 4 cut(s) 233, 241, 1501, 1646
CspAI ACCGGT 1 cut(s) 1590
CviAII CATG 9 cut(s) 37, 59, 122, 289, 1073, 1224, 1270, 1688, 2071
CviQI GTAC 4 cut(s) 233, 241, 1501, 1646
DdeI CTNAG 9 cut(s) 321, 402, 558, 640, 681, 849, 1383, 1462, 1563
DpnI GATC 3 cut(s) 483, 718, 1349
DpnII GATC 3 cut(s) 481, 716, 1347
DriI GACNNNNNGTC 1 cut(s) 1455
EaeI YGGCCR 1 cut(s) 2032
Eam1104I CTCTTC 2 cut(s) 762, 1440
Eam1105I GACNNNNNGTC 1 cut(s) 1455
EarI CTCTTC 2 cut(s) 762, 1440
Ecl136II GAGCTC 1 cut(s) 859
Eco130I CCWWGG 3 cut(s) 36, 58, 1072
Eco147I AGGCCT 1 cut(s) 1891
Eco24I GRGCYC 1 cut(s) 861
Eco31I GGTCTC 1 cut(s) 1442
Eco32I GATATC 1 cut(s) 211
Eco47I GGWCC 2 cut(s) 355, 1376
Eco53kI GAGCTC 1 cut(s) 859
Eco57I CTGAAG 2 cut(s) 714, 909
Eco81I CCTNAGG 1 cut(s) 849
Eco91I GGTNACC 1 cut(s) 1783
EcoICRI GAGCTC 1 cut(s) 859
EcoO109I RGGNCCY 1 cut(s) 355
EcoO65I GGTNACC 1 cut(s) 1783
EcoRII CCWGG 2 cut(s) 1369, 1417
EcoRV GATATC 1 cut(s) 211
EcoT14I CCWWGG 3 cut(s) 36, 58, 1072
EcoT38I GRGCYC 1 cut(s) 861
ErhI CCWWGG 3 cut(s) 36, 58, 1072
Esp3I CGTCTC 1 cut(s) 1607
FaeI CATG 9 cut(s) 40, 62, 125, 292, 1076, 1227, 1273, 1691, 2074
FaqI GGGAC 4 cut(s) 815, 1345, 1475, 1717
FatI CATG 9 cut(s) 36, 58, 121, 288, 1072, 1223, 1269, 1687, 2070
Fnu4HI GCNGC 7 cut(s) 318, 792, 908, 1185, 1808, 1943, 2045
FokI GGATG 5 cut(s) 136, 213, 1825, 2053, 2064
FriOI GRGCYC 1 cut(s) 861
Fsp4HI GCNGC 7 cut(s) 318, 792, 908, 1185, 1808, 1943, 2045
FspBI CTAG 5 cut(s) 168, 381, 1277, 2094, 2098
GlaI GCGC 1 cut(s) 1127
GluI GCNGC 7 cut(s) 318, 792, 908, 1185, 1808, 1943, 2045
GsaI CCCAGC 1 cut(s) 1935
HaeIII GGCC 5 cut(s) 63, 1116, 1339, 1891, 2034
HapII CCGG 4 cut(s) 485, 1120, 1452, 1591
HgaI GACGC 1 cut(s) 1132
HhaI GCGC 1 cut(s) 1128
Hin1I GRCGYC 1 cut(s) 1456
Hin1II CATG 9 cut(s) 40, 62, 125, 292, 1076, 1227, 1273, 1691, 2074
Hin6I GCGC 1 cut(s) 1126
HinP1I GCGC 1 cut(s) 1126
HindIII AAGCTT 1 cut(s) 1385
HinfI GANTC 7 cut(s) 125, 173, 518, 548, 683, 733, 875
HpaII CCGG 4 cut(s) 485, 1120, 1452, 1591
HphI GGTGA 6 cut(s) 971, 1088, 1149, 1630, 1636, 1795
Hpy166II GTNNAC 6 cut(s) 391, 978, 998, 1037, 1376, 1627
Hpy8I GTNNAC 6 cut(s) 391, 978, 998, 1037, 1376, 1627
Hpy99I CGWCG 3 cut(s) 529, 948, 1461
HpyAV CCTTC 6 cut(s) 346, 689, 928, 1729, 1862, 1963
HpyCH4III ACNGT 3 cut(s) 443, 1618, 1631
HpyCH4IV ACGT 2 cut(s) 1354, 1456
HpyF10VI GCNNNNNNNGC 3 cut(s) 314, 916, 1286
HpyF3I CTNAG 9 cut(s) 321, 402, 558, 640, 681, 849, 1383, 1462, 1563
HpySE526I ACGT 2 cut(s) 1354, 1456
Hsp92I GRCGYC 1 cut(s) 1456
Hsp92II CATG 9 cut(s) 40, 62, 125, 292, 1076, 1227, 1273, 1691, 2074
HspAI GCGC 1 cut(s) 1126
Kpn2I TCCGGA 2 cut(s) 484, 1119
Kzo9I GATC 3 cut(s) 481, 716, 1347
LmnI GCTCC 2 cut(s) 1057, 1720
Lsp1109I GCAGC 7 cut(s) 304, 778, 919, 1171, 1819, 1954, 2031
LweI GCATC 7 cut(s) 158, 552, 648, 686, 1069, 1630, 2031
MabI ACCWGGT 2 cut(s) 1369, 1417
MaeI CTAG 5 cut(s) 168, 381, 1277, 2094, 2098
MaeII ACGT 2 cut(s) 1354, 1456
MaeIII GTNAC 4 cut(s) 443, 911, 1242, 1783
MalI GATC 3 cut(s) 483, 718, 1349
MboI GATC 3 cut(s) 481, 716, 1347
MfeI CAATTG 2 cut(s) 653, 1579
MflI RGATCY 1 cut(s) 481
MhlI GDGCHC 5 cut(s) 393, 861, 980, 1000, 1062
MlsI TGGCCA 1 cut(s) 2034
MluCI AATT 9 cut(s) 50, 577, 653, 1536, 1551, 1579, 1665, 1848, 1921
MluNI TGGCCA 1 cut(s) 2034
MlyI GAGTC 4 cut(s) 527, 692, 727, 884
MmeI TCCRAC 1 cut(s) 1375
Mox20I TGGCCA 1 cut(s) 2034
MroI TCCGGA 2 cut(s) 484, 1119
MroXI GAANNNNTTC 1 cut(s) 329
MscI TGGCCA 1 cut(s) 2034
MseI TTAA 7 cut(s) 345, 462, 923, 1196, 1595, 1650, 1851
MslI CAYNNNNRTG 1 cut(s) 1077
Msp20I TGGCCA 1 cut(s) 2034
MspA1I CMGCKG 2 cut(s) 1184, 1810
MspCI CTTAAG 1 cut(s) 1649
MspI CCGG 4 cut(s) 485, 1120, 1452, 1591
MspR9I CCNGG 2 cut(s) 1371, 1419
MunI CAATTG 2 cut(s) 653, 1579
Mva1269I GAATGC 1 cut(s) 494
MvaI CCWGG 2 cut(s) 1371, 1419
MvnI CGCG 1 cut(s) 1126
MwoI GCNNNNNNNGC 3 cut(s) 314, 916, 1286
NcoI CCATGG 3 cut(s) 36, 58, 1072
NdeII GATC 3 cut(s) 481, 716, 1347
NheI GCTAGC 1 cut(s) 1276
NlaIII CATG 9 cut(s) 40, 62, 125, 292, 1076, 1227, 1273, 1691, 2074
NlaIV GGNNCC 4 cut(s) 357, 421, 483, 1138
NmuCI GTSAC 2 cut(s) 1242, 1783
NspI RCATGY 2 cut(s) 292, 1227
PaeI GCATGC 1 cut(s) 1227
PagI TCATGA 2 cut(s) 121, 1269
PceI AGGCCT 1 cut(s) 1891
PctI GAATGC 1 cut(s) 494
PdmI GAANNNNTTC 1 cut(s) 329
PfeI GAWTC 3 cut(s) 125, 173, 548
PflFI GACNNNGTC 1 cut(s) 1984
PinAI ACCGGT 1 cut(s) 1590
PkrI GCNGC 7 cut(s) 319, 793, 909, 1186, 1809, 1944, 2046
PleI GAGTC 4 cut(s) 526, 691, 727, 883
PpsI GAGTC 4 cut(s) 526, 691, 727, 883
PpuMI RGGWCCY 1 cut(s) 355
PshBI ATTAAT 1 cut(s) 1851
Psp124BI GAGCTC 1 cut(s) 861
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 2 cut(s) 1369, 1417
PspEI GGTNACC 1 cut(s) 1783
PspFI CCCAGC 1 cut(s) 1931
PspGI CCWGG 2 cut(s) 1369, 1417
PspN4I GGNNCC 4 cut(s) 357, 421, 483, 1138
PspPI GGNCC 3 cut(s) 62, 355, 1376
PspPPI RGGWCCY 1 cut(s) 355
PsrI GAACNNNNNNTAC 2 cut(s) 172, 204
PstI CTGCAG 1 cut(s) 2049
PstNI CAGNNNCTG 3 cut(s) 692, 1187, 1963
PsuI RGATCY 1 cut(s) 481
PsyI GACNNNGTC 1 cut(s) 1984
PvuII CAGCTG 1 cut(s) 1184
RsaI GTAC 4 cut(s) 234, 242, 1502, 1647
RsaNI GTAC 4 cut(s) 233, 241, 1501, 1646
RseI CAYNNNNRTG 1 cut(s) 1077
SacI GAGCTC 1 cut(s) 861
SaqAI TTAA 7 cut(s) 345, 462, 923, 1196, 1595, 1650, 1851
SatI GCNGC 7 cut(s) 318, 792, 908, 1185, 1808, 1943, 2045
Sau3AI GATC 3 cut(s) 481, 716, 1347
Sau96I GGNCC 3 cut(s) 62, 355, 1376
SchI GAGTC 4 cut(s) 527, 692, 727, 884
ScrFI CCNGG 2 cut(s) 1371, 1419
SduI GDGCHC 5 cut(s) 393, 861, 980, 1000, 1062
SexAI ACCWGGT 2 cut(s) 1369, 1417
SfaNI GCATC 7 cut(s) 158, 552, 648, 686, 1069, 1630, 2031
SfcI CTRYAG 3 cut(s) 795, 1431, 2045
SinI GGWCC 2 cut(s) 355, 1376
SmiMI CAYNNNNRTG 1 cut(s) 1077
SmlI CTYRAG 4 cut(s) 236, 663, 1043, 1649
SmoI CTYRAG 4 cut(s) 236, 663, 1043, 1649
SphI GCATGC 1 cut(s) 1227
Sse9I AATT 9 cut(s) 50, 577, 653, 1536, 1551, 1579, 1665, 1848, 1921
SseBI AGGCCT 1 cut(s) 1891
SsiI CCGC 1 cut(s) 1810
SspI AATATT 1 cut(s) 955
SspMI CTAG 5 cut(s) 168, 381, 1277, 2094, 2098
SstI GAGCTC 1 cut(s) 861
StuI AGGCCT 1 cut(s) 1891
StyD4I CCNGG 2 cut(s) 1369, 1417
StyI CCWWGG 3 cut(s) 36, 58, 1072
TaaI ACNGT 3 cut(s) 443, 1618, 1631
TaiI ACGT 2 cut(s) 1357, 1459
TaqI TCGA 3 cut(s) 527, 707, 1426
TasI AATT 9 cut(s) 50, 577, 653, 1536, 1551, 1579, 1665, 1848, 1921
TatI WGTACW 4 cut(s) 232, 240, 1500, 1645
TfiI GAWTC 3 cut(s) 125, 173, 548
Tru1I TTAA 7 cut(s) 345, 462, 923, 1196, 1595, 1650, 1851
Tru9I TTAA 7 cut(s) 345, 462, 923, 1196, 1595, 1650, 1851
TscAI CASTG 1 cut(s) 1913
TseFI GTSAC 2 cut(s) 1242, 1783
TseI GCWGC 7 cut(s) 317, 791, 907, 1184, 1807, 1942, 2044
Tsp45I GTSAC 2 cut(s) 1242, 1783
TspDTI ATGAA 7 cut(s) 138, 540, 912, 1286, 1672, 1676, 2040
TspGWI ACGGA 1 cut(s) 961
TspRI CASTG 1 cut(s) 1913
Tth111I GACNNNGTC 1 cut(s) 1984
Vha464I CTTAAG 1 cut(s) 1649
VneI GTGCAC 3 cut(s) 389, 976, 996
VpaK11BI GGWCC 2 cut(s) 355, 1376
VspI ATTAAT 1 cut(s) 1851
XapI RAATTY 4 cut(s) 50, 577, 1665, 1921
XceI RCATGY 2 cut(s) 292, 1227
XcmI CCANNNNNNNNNTGG 1 cut(s) 1370
XmnI GAANNNNTTC 1 cut(s) 329
XspI CTAG 5 cut(s) 168, 381, 1277, 2094, 2098
ZraI GACGTC 1 cut(s) 1457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.