Rroxscaffold_1G00015620

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000175
Physical Location & Seq
Reverse (-)
19396063 .. 19399172
3110 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00015620.1

Sequence Viewer

Length: 2094 bp
ATGGATACCCTTTACAGATATTCTGTCTCCAATCCCATGGAGAGGTTCAAATTTGACCATGGCCCAGTTTCAGTCTGTCCAAATCCCAATCTTGTAAATCGGTTCAAAATAAATCATGAATCCACCAATCCAGTATTACTTGCATCCAAATCTGATCTTTCTAGTGATTCAAGTAATGACGGTTCAGAGGTAGATGGTCTTGATATCAATGACTGCAACCATCCTGTACTCAAGTACATAAATGATATTCTTCTGGAAGAAGACTTGGAGGGTAAGCCCTGCATGTTGCAGGACTGTTTAGCCCTCCAAGCTGCTGAGAAGTCTTTCTATGATGTCCTTAATCAGAAGGACCCTCCTTTACCCGACCAACCCTCTAGTTATGTGCACCAAAGCTCTGAGAGTTCAGATGATGATTCCCCACAGAGTTTCCGAAACAGTAACGGCTCTTTTGCTGTTAAGACAGATTGGGTTTGGGATCCGGAATGCTCTCCCGTCCAATCTTCTCTATTTGAGTCTTCGTCGAATACCCGTTTGGTTTCTGATTCATTGTCTGAGATGCATAGTCTTGGGAATTTTGGAGGTGTGGGGGAAGCAAGCAAGTTCCTTTCAAATCTGAAACTTGAAATGTTTGACTTAGAGAGGTTTCAATTGATGCCTCAAGGTTCAGTCGCATCTGAGTCAGGGGCTGAAGGCTACAACTCGACCAATGGATCAAAGGGAAAGAAGACTCATCAAAGGGAGGATAGTCATTTTCCAGAAGAAGAGAGAAGCAGCAAGCAGTCAGCTGCTGTAGGGGACGACTTTGAGCCACAAGAAATGTTTGATGAGGTTTTGCTTTGTCCTGAGGTTGAGCTTTGTGCTGATTATGAGTCTTTGAAAACTGAAGCAAATGAAAAGTTGCAGCGTAACAAGCAGTTAAAAGGATTGAAGGCAAAGCGACAGAGTAATATTGGTGAAGTGGTAGATCTGTGCACAATGCTTACTCAATGTGCACAAGCTGTTGCGAACTTTGACCAACGAACTGCAAGTGAACTACTCAAGCAGATGAGGGAGCACACAACCCCCCATGGTGATGCAACCCAGAGGTTAGCCCATTACTTTGCTGATGGCCTCCAGATGCGCTTGGCTGGTGCCAAAACCCCATCATATTCACCTCTTGTAAGTGTGCAGACATCAGCTGCTGAAATCTTAAAAGCATACAAGGTATTTATTACAGCATGCCCTTTCAAGAAGATGTCACACTTCTTTGCCAACAGAACAATCATGAAGCTAGCTGAAAAAGCAACAAGGCTTCACATTATTGATTTTGGTATTTCTTATGGATTTCAATGGCCTTGCCTGATCCAACGTCTGTCCCAAAGACCTGGTGGACCTCCTAAGCTTCGTATCACAGCAATAGAGCTTCCCCAACCAGGTTTTCGACCTACAGAAAGAGTTGAAGAGACCGGACGTCGCTTAGAAAAATATGCCAAGAGATTTGATGTCCCATTTGAGTACAATGTCATTGCCCAGAAATGGGAAACTATCCGAATTGAGGACATCAAAATTGACAGAACTGAGCTGATTGTGGTCAATAGTTTGCACCGGTTAAGGAACATACCAGATGAGACGTTGATGGTGAACAGTCCAAGAGATGCTGTACTTAAGTTGATAAGGAAAATTTGTCCAGACCTTTTCATTCATGGTGTTGTCAATGGGACATACAATGCTCCCTTCTTTCTCACACGATTTAGAGAGGCACTCCTCCACTTCTATTCACTATTTGATATGCTTGAGGCGACCATCTCTCGTGAAGATGAGCCGCGGATGTTGTTTGAACAAGCAATATATGGTAGAGATATAATTAATGTCGTAGCGTGTGAAGGACTGGAAAGAGTTGAAAGGCCTGAAACATACAGGCAGTGGCAGGTTAGGAATTTGAGGGCTGGGTTCAAGCAGCTACCATTAGACCAGAAGCTGTTGAAGGAAGTGAAGACTATGTCAAAAGTAATGCGTTATCATAATGATTTTAGAGTTGATGAAGATGGCCATTGGATGCTGCAGGGATGGAAAGGAAGAACCCTCATGGCTCTTTCTTTTTGGAAGCCTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS RVT_2

Protein Analysis

697

Amino Acids

79.46

Weight (kDa)

5.82

Isoelectric Point (pI)

48.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 325 - 695 2.1e-120 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1453
Acc36I ACCTGC 1 cut(s) 1897
AccB1I GGYRCC 1 cut(s) 1130
AccII CGCG 1 cut(s) 1804
AccIII TCCGGA 1 cut(s) 478
AciI CCGC 2 cut(s) 1802, 1804
AclWI GGATC 4 cut(s) 470, 483, 718, 1336
AcoI YGGCCR 1 cut(s) 2026
AcsI RAATTY 4 cut(s) 50, 571, 1659, 1915
AcuI CTGAAG 2 cut(s) 708, 903
AcyI GRCGYC 1 cut(s) 1450
AfaI GTAC 4 cut(s) 228, 236, 1496, 1641
AfiI CCNNNNNNNGG 5 cut(s) 42, 1412, 1515, 1516, 1534
AflII CTTAAG 1 cut(s) 1643
AgeI ACCGGT 1 cut(s) 1584
AhdI GACNNNNNGTC 1 cut(s) 1449
AjnI CCWGG 2 cut(s) 1363, 1411
AjuI GAANNNNNNNTTGG 4 cut(s) 515, 547, 1401, 1433
Alw21I GWGCWC 4 cut(s) 387, 974, 994, 1056
Alw26I GTCTC 3 cut(s) 31, 1436, 1601
Alw44I GTGCAC 3 cut(s) 383, 970, 990
AlwI GGATC 4 cut(s) 470, 483, 718, 1336
AlwNI CAGNNNCTG 4 cut(s) 686, 788, 1181, 1957
Aor13HI TCCGGA 1 cut(s) 478
AoxI GGCC 5 cut(s) 61, 1108, 1331, 1883, 2026
ApaLI GTGCAC 3 cut(s) 383, 970, 990
ApeKI GCWGC 7 cut(s) 311, 771, 785, 901, 1178, 1936, 2038
ApoI RAATTY 4 cut(s) 50, 571, 1659, 1915
AseI ATTAAT 1 cut(s) 1845
AsiGI ACCGGT 1 cut(s) 1584
Asp700I GAANNNNTTC 1 cut(s) 323
AspLEI GCGC 1 cut(s) 1122
AspS9I GGNCC 3 cut(s) 62, 349, 1370
AsuHPI GGTGA 4 cut(s) 965, 1082, 1143, 1630
AsuNHI GCTAGC 1 cut(s) 1270
AvaII GGWCC 2 cut(s) 349, 1370
AxyI CCTNAGG 1 cut(s) 843
BaeGI GKGCMC 3 cut(s) 387, 974, 994
BalI TGGCCA 1 cut(s) 2028
BamHI GGATCC 1 cut(s) 475
BanI GGYRCC 1 cut(s) 1130
BauI CACGAG 1 cut(s) 1788
BbsI GAAGAC 4 cut(s) 267, 507, 731, 1979
Bbv12I GWGCWC 4 cut(s) 387, 974, 994, 1056
BbvI GCAGC 7 cut(s) 298, 772, 783, 913, 1165, 1948, 2025
BccI CCATC 8 cut(s) 188, 228, 1100, 1150, 1609, 1790, 2018, 2040
BceAI ACGGC 1 cut(s) 457
BciT130I CCWGG 2 cut(s) 1365, 1413
BcoDI GTCTC 3 cut(s) 31, 1436, 1601
BfaI CTAG 4 cut(s) 162, 375, 1271, 2092
BfmI CTRYAG 3 cut(s) 789, 1425, 2039
BfrI CTTAAG 1 cut(s) 1643
BfuAI ACCTGC 1 cut(s) 1897
BglII AGATCT 1 cut(s) 964
BisI GCNGC 8 cut(s) 312, 772, 786, 902, 1179, 1802, 1937, 2039
BlsI GCNGC 8 cut(s) 313, 773, 787, 903, 1180, 1803, 1938, 2040
Bme1390I CCNGG 2 cut(s) 1365, 1413
Bme18I GGWCC 2 cut(s) 349, 1370
BmeRI GACNNNNNGTC 1 cut(s) 1449
BmgT120I GGNCC 3 cut(s) 62, 349, 1370
BmiI GGNNCC 3 cut(s) 351, 477, 1132
BmrFI CCNGG 2 cut(s) 1365, 1413
BmrI ACTGGG 1 cut(s) 59
BmsI GCATC 8 cut(s) 152, 546, 642, 680, 1063, 1107, 1624, 2025
BmtI GCTAGC 1 cut(s) 1274
BmuI ACTGGG 1 cut(s) 59
BpiI GAAGAC 4 cut(s) 267, 507, 731, 1979
BplI GAGNNNNNCTC 2 cut(s) 1725, 1757
BpmI CTGGAG 1 cut(s) 1097
Bpu10I CCTNAGC 1 cut(s) 1377
BpuEI CTTGAG 4 cut(s) 215, 642, 1022, 1793
BsaHI GRCGYC 1 cut(s) 1450
BsaI GGTCTC 1 cut(s) 1436
BsaJI CCNNGG 4 cut(s) 36, 58, 1066, 1802
BsaWI WCCGGW 3 cut(s) 478, 1445, 1584
Bsc4I CCNNNNNNNGG 5 cut(s) 42, 1412, 1515, 1516, 1534
Bse118I RCCGGY 1 cut(s) 1584
Bse1I ACTGG 3 cut(s) 65, 131, 1872
Bse21I CCTNAGG 1 cut(s) 843
Bse3DI GCAATG 1 cut(s) 1503
BseAI TCCGGA 1 cut(s) 478
BseBI CCWGG 2 cut(s) 1365, 1413
BseDI CCNNGG 4 cut(s) 36, 58, 1066, 1802
BseGI GGATG 5 cut(s) 143, 220, 1812, 2040, 2051
BseLI CCNNNNNNNGG 5 cut(s) 42, 1412, 1515, 1516, 1534
BseMI GCAATG 1 cut(s) 1503
BseMII CTCAG 6 cut(s) 306, 387, 543, 666, 834, 1548
BseNI ACTGG 3 cut(s) 65, 131, 1872
BseRI GAGGAG 1 cut(s) 1733
BseSI GKGCMC 3 cut(s) 387, 974, 994
BseXI GCAGC 7 cut(s) 298, 772, 783, 913, 1165, 1948, 2025
BseYI CCCAGC 1 cut(s) 1925
BsgI GTGCAG 1 cut(s) 1187
Bsh1236I CGCG 1 cut(s) 1804
BshFI GGCC 5 cut(s) 63, 1110, 1333, 1885, 2028
BshNI GGYRCC 1 cut(s) 1130
BshTI ACCGGT 1 cut(s) 1584
BsiHKAI GWGCWC 4 cut(s) 387, 974, 994, 1056
BsiSI CCGG 3 cut(s) 479, 1446, 1585
BslFI GGGAC 4 cut(s) 809, 1339, 1469, 1711
BslI CCNNNNNNNGG 5 cut(s) 42, 1412, 1515, 1516, 1534
BsmAI GTCTC 3 cut(s) 31, 1436, 1601
BsmBI CGTCTC 1 cut(s) 1601
BsmFI GGGAC 4 cut(s) 809, 1339, 1469, 1711
BsmI GAATGC 1 cut(s) 488
BsnI GGCC 5 cut(s) 63, 1110, 1333, 1885, 2028
Bso31I GGTCTC 1 cut(s) 1436
Bsp1286I GDGCHC 4 cut(s) 387, 974, 994, 1056
Bsp13I TCCGGA 1 cut(s) 478
Bsp143I GATC 5 cut(s) 154, 475, 710, 964, 1341
Bsp19I CCATGG 3 cut(s) 36, 58, 1066
BspACI CCGC 2 cut(s) 1802, 1804
BspANI GGCC 5 cut(s) 63, 1110, 1333, 1885, 2028
BspCNI CTCAG 6 cut(s) 307, 388, 544, 667, 835, 1549
BspEI TCCGGA 1 cut(s) 478
BspFNI CGCG 1 cut(s) 1804
BspHI TCATGA 2 cut(s) 115, 1263
BspLI GGNNCC 3 cut(s) 351, 477, 1132
BspMAI CTGCAG 1 cut(s) 2043
BspMI ACCTGC 1 cut(s) 1897
BspOI GCTAGC 1 cut(s) 1274
BspPI GGATC 4 cut(s) 470, 483, 718, 1336
BspT107I GGYRCC 1 cut(s) 1130
BspTI CTTAAG 1 cut(s) 1643
BspTNI GGTCTC 1 cut(s) 1436
BsrDI GCAATG 1 cut(s) 1503
BsrFI RCCGGY 1 cut(s) 1584
BsrI ACTGG 3 cut(s) 65, 131, 1872
BssAI RCCGGY 1 cut(s) 1584
BssECI CCNNGG 4 cut(s) 36, 58, 1066, 1802
BssMI GATC 5 cut(s) 154, 475, 710, 964, 1341
BssNI GRCGYC 1 cut(s) 1450
BssSI CACGAG 1 cut(s) 1788
BssT1I CCWWGG 3 cut(s) 36, 58, 1066
Bst2BI CACGAG 1 cut(s) 1788
Bst2UI CCWGG 2 cut(s) 1365, 1413
Bst4CI ACNGT 4 cut(s) 182, 296, 437, 1625
Bst6I CTCTTC 2 cut(s) 756, 1434
BstACI GRCGYC 1 cut(s) 1450
BstAFI CTTAAG 1 cut(s) 1643
BstC8I GCNNGC 4 cut(s) 595, 776, 1219, 1272
BstDEI CTNAG 9 cut(s) 315, 396, 552, 634, 675, 843, 1377, 1456, 1557
BstDSI CCRYGG 4 cut(s) 36, 58, 1066, 1802
BstF5I GGATG 5 cut(s) 143, 220, 1812, 2040, 2051
BstFNI CGCG 1 cut(s) 1804
BstHHI GCGC 1 cut(s) 1122
BstKTI GATC 5 cut(s) 157, 478, 713, 967, 1344
BstMAI GTCTC 3 cut(s) 31, 1436, 1601
BstMBI GATC 5 cut(s) 154, 475, 710, 964, 1341
BstMWI GCNNNNNNNGC 3 cut(s) 308, 910, 1280
BstNI CCWGG 2 cut(s) 1365, 1413
BstNSI RCATGY 2 cut(s) 286, 1221
BstSCI CCNGG 2 cut(s) 1363, 1411
BstSFI CTRYAG 3 cut(s) 789, 1425, 2039
BstSLI GKGCMC 3 cut(s) 387, 974, 994
BstUI CGCG 1 cut(s) 1804
BstV1I GCAGC 7 cut(s) 298, 772, 783, 913, 1165, 1948, 2025
BstV2I GAAGAC 4 cut(s) 267, 507, 731, 1979
BstX2I RGATCY 2 cut(s) 475, 964
BstXI CCANNNNNNTGG 2 cut(s) 37, 1364
BstYI RGATCY 2 cut(s) 475, 964
Bsu36I CCTNAGG 1 cut(s) 843
BsuRI GGCC 5 cut(s) 63, 1110, 1333, 1885, 2028
BtgI CCRYGG 4 cut(s) 36, 58, 1066, 1802
BtsCI GGATG 5 cut(s) 143, 220, 1812, 2040, 2051
BtsI GCAGTG 1 cut(s) 1907
BtsIMutI CAGTG 1 cut(s) 1907
BveI ACCTGC 1 cut(s) 1897
Cac8I GCNNGC 4 cut(s) 595, 776, 1219, 1272
CaiI CAGNNNCTG 4 cut(s) 686, 788, 1181, 1957
CciI TCATGA 2 cut(s) 115, 1263
CfoI GCGC 1 cut(s) 1122
Cfr10I RCCGGY 1 cut(s) 1584
Cfr13I GGNCC 3 cut(s) 62, 349, 1370
Cfr42I CCGCGG 1 cut(s) 1805
CsiI ACCWGGT 2 cut(s) 1363, 1411
Csp6I GTAC 4 cut(s) 227, 235, 1495, 1640
CspAI ACCGGT 1 cut(s) 1584
CviAII CATG 9 cut(s) 37, 59, 116, 283, 1067, 1218, 1264, 1682, 2065
CviQI GTAC 4 cut(s) 227, 235, 1495, 1640
DdeI CTNAG 9 cut(s) 315, 396, 552, 634, 675, 843, 1377, 1456, 1557
DpnI GATC 5 cut(s) 156, 477, 712, 966, 1343
DpnII GATC 5 cut(s) 154, 475, 710, 964, 1341
DriI GACNNNNNGTC 1 cut(s) 1449
EaeI YGGCCR 1 cut(s) 2026
Eam1104I CTCTTC 2 cut(s) 756, 1434
Eam1105I GACNNNNNGTC 1 cut(s) 1449
EarI CTCTTC 2 cut(s) 756, 1434
Eco130I CCWWGG 3 cut(s) 36, 58, 1066
Eco147I AGGCCT 1 cut(s) 1885
Eco31I GGTCTC 1 cut(s) 1436
Eco32I GATATC 1 cut(s) 205
Eco47I GGWCC 2 cut(s) 349, 1370
Eco57I CTGAAG 2 cut(s) 708, 903
Eco81I CCTNAGG 1 cut(s) 843
EcoO109I RGGNCCY 1 cut(s) 349
EcoRII CCWGG 2 cut(s) 1363, 1411
EcoRV GATATC 1 cut(s) 205
EcoT14I CCWWGG 3 cut(s) 36, 58, 1066
EcoT22I ATGCAT 1 cut(s) 561
ErhI CCWWGG 3 cut(s) 36, 58, 1066
Esp3I CGTCTC 1 cut(s) 1601
FaeI CATG 9 cut(s) 40, 62, 119, 286, 1070, 1221, 1267, 1685, 2068
FaqI GGGAC 4 cut(s) 809, 1339, 1469, 1711
FatI CATG 9 cut(s) 36, 58, 115, 282, 1066, 1217, 1263, 1681, 2064
Fnu4HI GCNGC 8 cut(s) 312, 772, 786, 902, 1179, 1802, 1937, 2039
FokI GGATG 5 cut(s) 130, 207, 1819, 2047, 2058
Fsp4HI GCNGC 8 cut(s) 312, 772, 786, 902, 1179, 1802, 1937, 2039
FspBI CTAG 4 cut(s) 162, 375, 1271, 2092
GlaI GCGC 1 cut(s) 1121
GluI GCNGC 8 cut(s) 312, 772, 786, 902, 1179, 1802, 1937, 2039
GsaI CCCAGC 1 cut(s) 1929
GsuI CTGGAG 1 cut(s) 1097
HaeIII GGCC 5 cut(s) 63, 1110, 1333, 1885, 2028
HapII CCGG 3 cut(s) 479, 1446, 1585
HhaI GCGC 1 cut(s) 1122
Hin1I GRCGYC 1 cut(s) 1450
Hin1II CATG 9 cut(s) 40, 62, 119, 286, 1070, 1221, 1267, 1685, 2068
Hin6I GCGC 1 cut(s) 1120
HinP1I GCGC 1 cut(s) 1120
HindIII AAGCTT 1 cut(s) 1379
HinfI GANTC 8 cut(s) 119, 167, 413, 512, 542, 677, 727, 869
HpaII CCGG 3 cut(s) 479, 1446, 1585
HphI GGTGA 4 cut(s) 965, 1082, 1143, 1630
Hpy166II GTNNAC 6 cut(s) 385, 972, 992, 1031, 1370, 1621
Hpy8I GTNNAC 6 cut(s) 385, 972, 992, 1031, 1370, 1621
Hpy99I CGWCG 2 cut(s) 523, 1455
HpyAV CCTTC 6 cut(s) 340, 683, 922, 1723, 1856, 1957
HpyCH4III ACNGT 4 cut(s) 182, 296, 437, 1625
HpyCH4IV ACGT 3 cut(s) 1348, 1450, 1610
HpyF10VI GCNNNNNNNGC 3 cut(s) 308, 910, 1280
HpyF3I CTNAG 9 cut(s) 315, 396, 552, 634, 675, 843, 1377, 1456, 1557
HpySE526I ACGT 3 cut(s) 1348, 1450, 1610
Hsp92I GRCGYC 1 cut(s) 1450
Hsp92II CATG 9 cut(s) 40, 62, 119, 286, 1070, 1221, 1267, 1685, 2068
HspAI GCGC 1 cut(s) 1120
Kpn2I TCCGGA 1 cut(s) 478
KspI CCGCGG 1 cut(s) 1805
Kzo9I GATC 5 cut(s) 154, 475, 710, 964, 1341
LmnI GCTCC 2 cut(s) 1051, 1714
Lsp1109I GCAGC 7 cut(s) 298, 772, 783, 913, 1165, 1948, 2025
LweI GCATC 8 cut(s) 152, 546, 642, 680, 1063, 1107, 1624, 2025
MabI ACCWGGT 2 cut(s) 1363, 1411
MaeI CTAG 4 cut(s) 162, 375, 1271, 2092
MaeII ACGT 3 cut(s) 1348, 1450, 1610
MaeIII GTNAC 3 cut(s) 437, 905, 1236
MalI GATC 5 cut(s) 156, 477, 712, 966, 1343
MboI GATC 5 cut(s) 154, 475, 710, 964, 1341
MfeI CAATTG 1 cut(s) 647
MflI RGATCY 2 cut(s) 475, 964
MhlI GDGCHC 4 cut(s) 387, 974, 994, 1056
MlsI TGGCCA 1 cut(s) 2028
MluCI AATT 8 cut(s) 50, 571, 647, 1530, 1545, 1659, 1842, 1915
MluNI TGGCCA 1 cut(s) 2028
MlyI GAGTC 4 cut(s) 521, 686, 721, 878
MmeI TCCRAC 1 cut(s) 1369
Mox20I TGGCCA 1 cut(s) 2028
Mph1103I ATGCAT 1 cut(s) 561
MroI TCCGGA 1 cut(s) 478
MroXI GAANNNNTTC 1 cut(s) 323
MscI TGGCCA 1 cut(s) 2028
MseI TTAA 7 cut(s) 339, 456, 917, 1190, 1589, 1644, 1845
MslI CAYNNNNRTG 1 cut(s) 1071
Msp20I TGGCCA 1 cut(s) 2028
MspA1I CMGCKG 3 cut(s) 785, 1178, 1804
MspCI CTTAAG 1 cut(s) 1643
MspI CCGG 3 cut(s) 479, 1446, 1585
MspR9I CCNGG 2 cut(s) 1365, 1413
MunI CAATTG 1 cut(s) 647
Mva1269I GAATGC 1 cut(s) 488
MvaI CCWGG 2 cut(s) 1365, 1413
MvnI CGCG 1 cut(s) 1804
MwoI GCNNNNNNNGC 3 cut(s) 308, 910, 1280
NcoI CCATGG 3 cut(s) 36, 58, 1066
NdeII GATC 5 cut(s) 154, 475, 710, 964, 1341
NheI GCTAGC 1 cut(s) 1270
NlaIII CATG 9 cut(s) 40, 62, 119, 286, 1070, 1221, 1267, 1685, 2068
NlaIV GGNNCC 3 cut(s) 351, 477, 1132
NmuCI GTSAC 1 cut(s) 1236
NsiI ATGCAT 1 cut(s) 561
NspI RCATGY 2 cut(s) 286, 1221
PaeI GCATGC 1 cut(s) 1221
PagI TCATGA 2 cut(s) 115, 1263
PceI AGGCCT 1 cut(s) 1885
PctI GAATGC 1 cut(s) 488
PdmI GAANNNNTTC 1 cut(s) 323
PfeI GAWTC 4 cut(s) 119, 167, 413, 542
PflFI GACNNNGTC 1 cut(s) 1978
PinAI ACCGGT 1 cut(s) 1584
PkrI GCNGC 8 cut(s) 313, 773, 787, 903, 1180, 1803, 1938, 2040
PleI GAGTC 4 cut(s) 520, 685, 721, 877
PpsI GAGTC 4 cut(s) 520, 685, 721, 877
PpuMI RGGWCCY 1 cut(s) 349
PshBI ATTAAT 1 cut(s) 1845
Psp5II RGGWCCY 1 cut(s) 349
Psp6I CCWGG 2 cut(s) 1363, 1411
PspFI CCCAGC 1 cut(s) 1925
PspGI CCWGG 2 cut(s) 1363, 1411
PspN4I GGNNCC 3 cut(s) 351, 477, 1132
PspPI GGNCC 3 cut(s) 62, 349, 1370
PspPPI RGGWCCY 1 cut(s) 349
PsrI GAACNNNNNNTAC 2 cut(s) 166, 198
PstI CTGCAG 1 cut(s) 2043
PstNI CAGNNNCTG 4 cut(s) 686, 788, 1181, 1957
PsuI RGATCY 2 cut(s) 475, 964
PsyI GACNNNGTC 1 cut(s) 1978
PvuII CAGCTG 2 cut(s) 785, 1178
RsaI GTAC 4 cut(s) 228, 236, 1496, 1641
RsaNI GTAC 4 cut(s) 227, 235, 1495, 1640
RseI CAYNNNNRTG 1 cut(s) 1071
SacII CCGCGG 1 cut(s) 1805
SaqAI TTAA 7 cut(s) 339, 456, 917, 1190, 1589, 1644, 1845
SatI GCNGC 8 cut(s) 312, 772, 786, 902, 1179, 1802, 1937, 2039
Sau3AI GATC 5 cut(s) 154, 475, 710, 964, 1341
Sau96I GGNCC 3 cut(s) 62, 349, 1370
SchI GAGTC 4 cut(s) 521, 686, 721, 878
ScrFI CCNGG 2 cut(s) 1365, 1413
SduI GDGCHC 4 cut(s) 387, 974, 994, 1056
SexAI ACCWGGT 2 cut(s) 1363, 1411
SfaNI GCATC 8 cut(s) 152, 546, 642, 680, 1063, 1107, 1624, 2025
SfcI CTRYAG 3 cut(s) 789, 1425, 2039
Sfr303I CCGCGG 1 cut(s) 1805
SgrBI CCGCGG 1 cut(s) 1805
SinI GGWCC 2 cut(s) 349, 1370
SmiMI CAYNNNNRTG 1 cut(s) 1071
SmlI CTYRAG 5 cut(s) 230, 657, 1037, 1643, 1772
SmoI CTYRAG 5 cut(s) 230, 657, 1037, 1643, 1772
SphI GCATGC 1 cut(s) 1221
Sse9I AATT 8 cut(s) 50, 571, 647, 1530, 1545, 1659, 1842, 1915
SseBI AGGCCT 1 cut(s) 1885
SsiI CCGC 2 cut(s) 1802, 1804
SspI AATATT 1 cut(s) 949
SspMI CTAG 4 cut(s) 162, 375, 1271, 2092
StuI AGGCCT 1 cut(s) 1885
StyD4I CCNGG 2 cut(s) 1363, 1411
StyI CCWWGG 3 cut(s) 36, 58, 1066
TaaI ACNGT 4 cut(s) 182, 296, 437, 1625
TaiI ACGT 3 cut(s) 1351, 1453, 1613
TaqI TCGA 3 cut(s) 521, 701, 1420
TasI AATT 8 cut(s) 50, 571, 647, 1530, 1545, 1659, 1842, 1915
TatI WGTACW 4 cut(s) 226, 234, 1494, 1639
TauI GCSGC 1 cut(s) 1804
TfiI GAWTC 4 cut(s) 119, 167, 413, 542
Tru1I TTAA 7 cut(s) 339, 456, 917, 1190, 1589, 1644, 1845
Tru9I TTAA 7 cut(s) 339, 456, 917, 1190, 1589, 1644, 1845
TscAI CASTG 1 cut(s) 1907
TseFI GTSAC 1 cut(s) 1236
TseI GCWGC 7 cut(s) 311, 771, 785, 901, 1178, 1936, 2038
Tsp45I GTSAC 1 cut(s) 1236
TspDTI ATGAA 7 cut(s) 132, 534, 906, 1280, 1666, 1670, 2034
TspRI CASTG 1 cut(s) 1907
Tth111I GACNNNGTC 1 cut(s) 1978
Vha464I CTTAAG 1 cut(s) 1643
VneI GTGCAC 3 cut(s) 383, 970, 990
VpaK11BI GGWCC 2 cut(s) 349, 1370
VspI ATTAAT 1 cut(s) 1845
XapI RAATTY 4 cut(s) 50, 571, 1659, 1915
XceI RCATGY 2 cut(s) 286, 1221
XcmI CCANNNNNNNNNTGG 1 cut(s) 1364
XmnI GAANNNNTTC 1 cut(s) 323
XspI CTAG 4 cut(s) 162, 375, 1271, 2092
ZraI GACGTC 1 cut(s) 1451
Zsp2I ATGCAT 1 cut(s) 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.