Belongs to the GRAS family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1050 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1567 |
| AccIII | TCCGGA | 1 cut(s) | 1250 |
| AciI | CCGC | 4 cut(s) | 390, 663, 1013, 1336 |
| AclWI | GGATC | 2 cut(s) | 59, 711 |
| AcsI | RAATTY | 2 cut(s) | 56, 1283 |
| AcuI | CTGAAG | 2 cut(s) | 215, 731 |
| AfaI | GTAC | 4 cut(s) | 711, 1025, 1379, 1466 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 11, 1295, 1567 |
| AjnI | CCWGG | 2 cut(s) | 1748, 1830 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 82, 114, 472, 504, 1284, 1316 |
| Alw21I | GWGCWC | 1 cut(s) | 137 |
| Alw26I | GTCTC | 2 cut(s) | 1319, 1652 |
| AlwI | GGATC | 2 cut(s) | 59, 711 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 712, 1064, 1687 |
| Aor13HI | TCCGGA | 1 cut(s) | 1250 |
| AoxI | GGCC | 5 cut(s) | 243, 988, 1214, 1763, 1930 |
| ApeKI | GCWGC | 6 cut(s) | 674, 931, 1010, 1061, 1174, 1687 |
| ApoI | RAATTY | 2 cut(s) | 56, 1283 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 447 |
| AspS9I | GGNCC | 2 cut(s) | 244, 1253 |
| AsuC2I | CCSGG | 1 cut(s) | 1613 |
| AsuHPI | GGTGA | 3 cut(s) | 302, 638, 962 |
| AsuNHI | GCTAGC | 1 cut(s) | 1153 |
| AvaII | GGWCC | 1 cut(s) | 1253 |
| BanII | GRGCYC | 1 cut(s) | 137 |
| BauI | CACGAG | 1 cut(s) | 102 |
| BbsI | GAAGAC | 1 cut(s) | 210 |
| Bbv12I | GWGCWC | 1 cut(s) | 137 |
| BbvI | GCAGC | 6 cut(s) | 686, 943, 997, 1048, 1161, 1674 |
| BccI | CCATC | 7 cut(s) | 88, 401, 563, 617, 947, 1895, 1917 |
| BciT130I | CCWGG | 2 cut(s) | 1750, 1832 |
| BciVI | GTATCC | 1 cut(s) | 1507 |
| BclI | TGATCA | 2 cut(s) | 40, 757 |
| BcnI | CCSGG | 1 cut(s) | 1613 |
| BcoDI | GTCTC | 2 cut(s) | 1319, 1652 |
| BfaI | CTAG | 6 cut(s) | 281, 393, 440, 1154, 1340, 1800 |
| BfmI | CTRYAG | 2 cut(s) | 1449, 1916 |
| BfuI | GTATCC | 1 cut(s) | 1507 |
| BisI | GCNGC | 7 cut(s) | 390, 675, 932, 1011, 1062, 1175, 1688 |
| BlsI | GCNGC | 7 cut(s) | 391, 676, 933, 1012, 1063, 1176, 1689 |
| Bme1390I | CCNGG | 3 cut(s) | 1613, 1750, 1832 |
| Bme18I | GGWCC | 1 cut(s) | 1253 |
| BmgT120I | GGNCC | 2 cut(s) | 244, 1253 |
| BmiI | GGNNCC | 7 cut(s) | 958, 1017, 1334, 1579, 1610, 1747, 1913 |
| BmrFI | CCNGG | 3 cut(s) | 1613, 1750, 1832 |
| BmrI | ACTGGG | 2 cut(s) | 63, 409 |
| BmsI | GCATC | 2 cut(s) | 180, 1221 |
| BmtI | GCTAGC | 1 cut(s) | 1157 |
| BmuI | ACTGGG | 2 cut(s) | 63, 409 |
| BpiI | GAAGAC | 1 cut(s) | 210 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 137, 169 |
| Bpu10I | CCTNAGC | 1 cut(s) | 412 |
| BpuEI | CTTGAG | 1 cut(s) | 902 |
| BpuMI | CCSGG | 1 cut(s) | 1613 |
| Bsa29I | ATCGAT | 1 cut(s) | 1185 |
| BsaJI | CCNNGG | 4 cut(s) | 551, 946, 1508, 1561 |
| BsaWI | WCCGGW | 2 cut(s) | 1030, 1250 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 825, 855 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 11, 1295, 1567 |
| Bse1I | ACTGG | 3 cut(s) | 69, 404, 1279 |
| BseAI | TCCGGA | 1 cut(s) | 1250 |
| BseBI | CCWGG | 2 cut(s) | 1750, 1832 |
| BseCI | ATCGAT | 1 cut(s) | 1185 |
| BseDI | CCNNGG | 4 cut(s) | 551, 946, 1508, 1561 |
| BseGI | GGATG | 7 cut(s) | 80, 409, 574, 628, 829, 1557, 1928 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 11, 1295, 1567 |
| BseMII | CTCAG | 7 cut(s) | 150, 249, 344, 426, 555, 675, 747 |
| BseNI | ACTGG | 3 cut(s) | 69, 404, 1279 |
| BseRI | GAGGAG | 2 cut(s) | 661, 1670 |
| BseXI | GCAGC | 6 cut(s) | 686, 943, 997, 1048, 1161, 1674 |
| BseYI | CCCAGC | 1 cut(s) | 1805 |
| BsgI | GTGCAG | 1 cut(s) | 1160 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1307 |
| BshFI | GGCC | 5 cut(s) | 245, 990, 1216, 1765, 1932 |
| BshVI | ATCGAT | 1 cut(s) | 1185 |
| BsiEI | CGRYCG | 1 cut(s) | 1307 |
| BsiHKAI | GWGCWC | 1 cut(s) | 137 |
| BsiSI | CCGG | 3 cut(s) | 1031, 1251, 1612 |
| BslFI | GGGAC | 3 cut(s) | 542, 1352, 1415 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 11, 1295, 1567 |
| BsmAI | GTCTC | 2 cut(s) | 1319, 1652 |
| BsmBI | CGTCTC | 1 cut(s) | 1319 |
| BsmFI | GGGAC | 3 cut(s) | 542, 1352, 1415 |
| BsnI | GGCC | 5 cut(s) | 245, 990, 1216, 1765, 1932 |
| Bsp1286I | GDGCHC | 1 cut(s) | 137 |
| Bsp13I | TCCGGA | 1 cut(s) | 1250 |
| Bsp1407I | TGTACA | 2 cut(s) | 1023, 1464 |
| Bsp19I | CCATGG | 2 cut(s) | 946, 1561 |
| BspACI | CCGC | 4 cut(s) | 390, 663, 1013, 1336 |
| BspANI | GGCC | 5 cut(s) | 245, 990, 1216, 1765, 1932 |
| BspCNI | CTCAG | 7 cut(s) | 149, 250, 343, 425, 556, 676, 746 |
| BspDI | ATCGAT | 1 cut(s) | 1185 |
| BspEI | TCCGGA | 1 cut(s) | 1250 |
| BspHI | TCATGA | 1 cut(s) | 52 |
| BspLI | GGNNCC | 7 cut(s) | 958, 1017, 1334, 1579, 1610, 1747, 1913 |
| BspMAI | CTGCAG | 1 cut(s) | 1920 |
| BspOI | GCTAGC | 1 cut(s) | 1157 |
| BspPI | GGATC | 2 cut(s) | 59, 711 |
| BsrGI | TGTACA | 2 cut(s) | 1023, 1464 |
| BsrI | ACTGG | 3 cut(s) | 69, 404, 1279 |
| BssECI | CCNNGG | 4 cut(s) | 551, 946, 1508, 1561 |
| BssSI | CACGAG | 1 cut(s) | 102 |
| BssT1I | CCWWGG | 4 cut(s) | 551, 946, 1508, 1561 |
| Bst2BI | CACGAG | 1 cut(s) | 102 |
| Bst2UI | CCWGG | 2 cut(s) | 1750, 1832 |
| Bst4CI | ACNGT | 7 cut(s) | 239, 1308, 1450, 1495, 1505, 1519, 1608 |
| Bst6I | CTCTTC | 3 cut(s) | 750, 1317, 1639 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 671 |
| BstAUI | TGTACA | 2 cut(s) | 1023, 1464 |
| BstC8I | GCNNGC | 5 cut(s) | 227, 886, 1102, 1155, 1236 |
| BstDEI | CTNAG | 7 cut(s) | 136, 258, 330, 412, 564, 684, 733 |
| BstDSI | CCRYGG | 2 cut(s) | 946, 1561 |
| BstF5I | GGATG | 7 cut(s) | 80, 409, 574, 628, 829, 1557, 1928 |
| BstMAI | GTCTC | 2 cut(s) | 1319, 1652 |
| BstMCI | CGRYCG | 1 cut(s) | 1307 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 251, 378, 556, 671, 1586, 1762 |
| BstNI | CCWGG | 2 cut(s) | 1750, 1832 |
| BstNSI | RCATGY | 4 cut(s) | 229, 1104, 1740, 1946 |
| BstSCI | CCNGG | 3 cut(s) | 1611, 1748, 1830 |
| BstSFI | CTRYAG | 2 cut(s) | 1449, 1916 |
| BstV1I | GCAGC | 6 cut(s) | 686, 943, 997, 1048, 1161, 1674 |
| BstV2I | GAAGAC | 1 cut(s) | 210 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 953 |
| Bsu15I | ATCGAT | 1 cut(s) | 1185 |
| BsuI | GTATCC | 1 cut(s) | 1507 |
| BsuRI | GGCC | 5 cut(s) | 245, 990, 1216, 1765, 1932 |
| BsuTUI | ATCGAT | 1 cut(s) | 1185 |
| BtgI | CCRYGG | 2 cut(s) | 946, 1561 |
| BtsCI | GGATG | 7 cut(s) | 80, 409, 574, 628, 829, 1557, 1928 |
| BtsI | GCAGTG | 2 cut(s) | 384, 1787 |
| BtsIMutI | CAGTG | 5 cut(s) | 67, 255, 384, 620, 1787 |
| Cac8I | GCNNGC | 5 cut(s) | 227, 886, 1102, 1155, 1236 |
| CaiI | CAGNNNCTG | 3 cut(s) | 712, 1064, 1687 |
| CciI | TCATGA | 1 cut(s) | 52 |
| Cfr13I | GGNCC | 2 cut(s) | 244, 1253 |
| ClaI | ATCGAT | 1 cut(s) | 1185 |
| Csp6I | GTAC | 4 cut(s) | 710, 1024, 1378, 1465 |
| CviQI | GTAC | 4 cut(s) | 710, 1024, 1378, 1465 |
| DdeI | CTNAG | 7 cut(s) | 136, 258, 330, 412, 564, 684, 733 |
| Eam1104I | CTCTTC | 3 cut(s) | 750, 1317, 1639 |
| EarI | CTCTTC | 3 cut(s) | 750, 1317, 1639 |
| Ecl136II | GAGCTC | 1 cut(s) | 135 |
| Eco130I | CCWWGG | 4 cut(s) | 551, 946, 1508, 1561 |
| Eco147I | AGGCCT | 1 cut(s) | 1765 |
| Eco24I | GRGCYC | 1 cut(s) | 137 |
| Eco32I | GATATC | 4 cut(s) | 348, 1069, 1423, 1552 |
| Eco47I | GGWCC | 1 cut(s) | 1253 |
| Eco53kI | GAGCTC | 1 cut(s) | 135 |
| Eco57I | CTGAAG | 2 cut(s) | 215, 731 |
| EcoICRI | GAGCTC | 1 cut(s) | 135 |
| EcoRI | GAATTC | 1 cut(s) | 56 |
| EcoRII | CCWGG | 2 cut(s) | 1748, 1830 |
| EcoRV | GATATC | 4 cut(s) | 348, 1069, 1423, 1552 |
| EcoT14I | CCWWGG | 4 cut(s) | 551, 946, 1508, 1561 |
| EcoT38I | GRGCYC | 1 cut(s) | 137 |
| ErhI | CCWWGG | 4 cut(s) | 551, 946, 1508, 1561 |
| Esp3I | CGTCTC | 1 cut(s) | 1319 |
| FaqI | GGGAC | 3 cut(s) | 542, 1352, 1415 |
| FbaI | TGATCA | 2 cut(s) | 40, 757 |
| Fnu4HI | GCNGC | 7 cut(s) | 390, 675, 932, 1011, 1062, 1175, 1688 |
| FokI | GGATG | 7 cut(s) | 67, 416, 581, 635, 836, 1544, 1935 |
| FriOI | GRGCYC | 1 cut(s) | 137 |
| Fsp4HI | GCNGC | 7 cut(s) | 390, 675, 932, 1011, 1062, 1175, 1688 |
| FspBI | CTAG | 6 cut(s) | 281, 393, 440, 1154, 1340, 1800 |
| GluI | GCNGC | 7 cut(s) | 390, 675, 932, 1011, 1062, 1175, 1688 |
| GsaI | CCCAGC | 1 cut(s) | 1809 |
| HaeIII | GGCC | 5 cut(s) | 245, 990, 1216, 1765, 1932 |
| HapII | CCGG | 3 cut(s) | 1031, 1251, 1612 |
| HincII | GTYRAC | 1 cut(s) | 1446 |
| HindII | GTYRAC | 1 cut(s) | 1446 |
| HindIII | AAGCTT | 2 cut(s) | 1077, 1526 |
| HinfI | GANTC | 9 cut(s) | 35, 110, 443, 530, 578, 680, 752, 1359, 1539 |
| HpaII | CCGG | 3 cut(s) | 1031, 1251, 1612 |
| HphI | GGTGA | 3 cut(s) | 302, 638, 962 |
| Hpy166II | GTNNAC | 3 cut(s) | 1446, 1456, 1894 |
| Hpy188I | TCNGA | 8 cut(s) | 18, 40, 139, 189, 352, 685, 757, 852 |
| Hpy8I | GTNNAC | 3 cut(s) | 1446, 1456, 1894 |
| HpyAV | CCTTC | 5 cut(s) | 108, 306, 323, 1227, 1603 |
| HpyCH4III | ACNGT | 7 cut(s) | 239, 1308, 1450, 1495, 1505, 1519, 1608 |
| HpyCH4V | TGCA | 9 cut(s) | 211, 225, 674, 872, 884, 1177, 1353, 1589, 1918 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 251, 378, 556, 671, 1586, 1762 |
| HpyF3I | CTNAG | 7 cut(s) | 136, 258, 330, 412, 564, 684, 733 |
| Kpn2I | TCCGGA | 1 cut(s) | 1250 |
| Ksp22I | TGATCA | 2 cut(s) | 40, 757 |
| LmnI | GCTCC | 7 cut(s) | 386, 556, 597, 648, 833, 1577, 1917 |
| Lsp1109I | GCAGC | 6 cut(s) | 686, 943, 997, 1048, 1161, 1674 |
| LweI | GCATC | 2 cut(s) | 180, 1221 |
| MaeI | CTAG | 6 cut(s) | 281, 393, 440, 1154, 1340, 1800 |
| MaeIII | GTNAC | 3 cut(s) | 28, 1165, 1818 |
| MhlI | GDGCHC | 1 cut(s) | 137 |
| MluCI | AATT | 4 cut(s) | 56, 1283, 1381, 1407 |
| MlyI | GAGTC | 2 cut(s) | 524, 572 |
| MmeI | TCCRAC | 2 cut(s) | 398, 681 |
| MroI | TCCGGA | 1 cut(s) | 1250 |
| MroXI | GAANNNNTTC | 1 cut(s) | 447 |
| MseI | TTAA | 5 cut(s) | 363, 765, 791, 1572, 1851 |
| MslI | CAYNNNNRTG | 2 cut(s) | 51, 951 |
| MspA1I | CMGCKG | 2 cut(s) | 677, 1061 |
| MspI | CCGG | 3 cut(s) | 1031, 1251, 1612 |
| MspR9I | CCNGG | 3 cut(s) | 1613, 1750, 1832 |
| MvaI | CCWGG | 2 cut(s) | 1750, 1832 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 251, 378, 556, 671, 1586, 1762 |
| NciI | CCSGG | 1 cut(s) | 1613 |
| NcoI | CCATGG | 2 cut(s) | 946, 1561 |
| NheI | GCTAGC | 1 cut(s) | 1153 |
| NlaIV | GGNNCC | 7 cut(s) | 958, 1017, 1334, 1579, 1610, 1747, 1913 |
| NspI | RCATGY | 4 cut(s) | 229, 1104, 1740, 1946 |
| PaeI | GCATGC | 2 cut(s) | 229, 1104 |
| PagI | TCATGA | 1 cut(s) | 52 |
| PceI | AGGCCT | 1 cut(s) | 1765 |
| PdmI | GAANNNNTTC | 1 cut(s) | 447 |
| PfeI | GAWTC | 7 cut(s) | 35, 110, 443, 680, 752, 1359, 1539 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1567 |
| PfoI | TCCNGGA | 2 cut(s) | 1611, 1748 |
| PkrI | GCNGC | 7 cut(s) | 391, 676, 933, 1012, 1063, 1176, 1689 |
| PleI | GAGTC | 2 cut(s) | 524, 572 |
| PpsI | GAGTC | 2 cut(s) | 524, 572 |
| PsiI | TTATAA | 1 cut(s) | 1050 |
| Psp124BI | GAGCTC | 1 cut(s) | 137 |
| Psp6I | CCWGG | 2 cut(s) | 1748, 1830 |
| PspFI | CCCAGC | 1 cut(s) | 1805 |
| PspGI | CCWGG | 2 cut(s) | 1748, 1830 |
| PspN4I | GGNNCC | 7 cut(s) | 958, 1017, 1334, 1579, 1610, 1747, 1913 |
| PspPI | GGNCC | 2 cut(s) | 244, 1253 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1448, 1480 |
| PstI | CTGCAG | 1 cut(s) | 1920 |
| PstNI | CAGNNNCTG | 3 cut(s) | 712, 1064, 1687 |
| PvuII | CAGCTG | 2 cut(s) | 677, 1061 |
| RsaI | GTAC | 4 cut(s) | 711, 1025, 1379, 1466 |
| RsaNI | GTAC | 4 cut(s) | 710, 1024, 1378, 1465 |
| RseI | CAYNNNNRTG | 2 cut(s) | 51, 951 |
| SacI | GAGCTC | 1 cut(s) | 137 |
| SaqAI | TTAA | 5 cut(s) | 363, 765, 791, 1572, 1851 |
| SatI | GCNGC | 7 cut(s) | 390, 675, 932, 1011, 1062, 1175, 1688 |
| Sau96I | GGNCC | 2 cut(s) | 244, 1253 |
| SbfI | CCTGCAGG | 1 cut(s) | 1920 |
| SchI | GAGTC | 2 cut(s) | 524, 572 |
| ScrFI | CCNGG | 3 cut(s) | 1613, 1750, 1832 |
| SdaI | CCTGCAGG | 1 cut(s) | 1920 |
| SduI | GDGCHC | 1 cut(s) | 137 |
| SfaNI | GCATC | 2 cut(s) | 180, 1221 |
| SfcI | CTRYAG | 2 cut(s) | 1449, 1916 |
| SinI | GGWCC | 1 cut(s) | 1253 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 51, 951 |
| SmlI | CTYRAG | 1 cut(s) | 917 |
| SmoI | CTYRAG | 1 cut(s) | 917 |
| SphI | GCATGC | 2 cut(s) | 229, 1104 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 1920 |
| Sse9I | AATT | 4 cut(s) | 56, 1283, 1381, 1407 |
| SseBI | AGGCCT | 1 cut(s) | 1765 |
| SsiI | CCGC | 4 cut(s) | 390, 663, 1013, 1336 |
| SspI | AATATT | 1 cut(s) | 1858 |
| SspMI | CTAG | 6 cut(s) | 281, 393, 440, 1154, 1340, 1800 |
| SstI | GAGCTC | 1 cut(s) | 137 |
| StuI | AGGCCT | 1 cut(s) | 1765 |
| StyD4I | CCNGG | 3 cut(s) | 1611, 1748, 1830 |
| StyI | CCWWGG | 4 cut(s) | 551, 946, 1508, 1561 |
| TaaI | ACNGT | 7 cut(s) | 239, 1308, 1450, 1495, 1505, 1519, 1608 |
| TaqI | TCGA | 4 cut(s) | 302, 993, 1185, 1303 |
| TasI | AATT | 4 cut(s) | 56, 1283, 1381, 1407 |
| TatI | WGTACW | 3 cut(s) | 1023, 1377, 1464 |
| TauI | GCSGC | 1 cut(s) | 392 |
| TfiI | GAWTC | 7 cut(s) | 35, 110, 443, 680, 752, 1359, 1539 |
| Tru1I | TTAA | 5 cut(s) | 363, 765, 791, 1572, 1851 |
| Tru9I | TTAA | 5 cut(s) | 363, 765, 791, 1572, 1851 |
| TscAI | CASTG | 5 cut(s) | 67, 262, 391, 620, 1787 |
| TseI | GCWGC | 6 cut(s) | 674, 931, 1010, 1061, 1174, 1687 |
| TspDTI | ATGAA | 9 cut(s) | 69, 102, 108, 349, 924, 1375, 1431, 1503, 1546 |
| TspGWI | ACGGA | 1 cut(s) | 846 |
| TspRI | CASTG | 5 cut(s) | 67, 262, 391, 620, 1787 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1567 |
| VpaK11BI | GGWCC | 1 cut(s) | 1253 |
| XapI | RAATTY | 2 cut(s) | 56, 1283 |
| XbaI | TCTAGA | 1 cut(s) | 439 |
| XceI | RCATGY | 4 cut(s) | 229, 1104, 1740, 1946 |
| XmnI | GAANNNNTTC | 1 cut(s) | 447 |
| XspI | CTAG | 6 cut(s) | 281, 393, 440, 1154, 1340, 1800 |