Rh5BG447200

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000175
Physical Location & Seq
Forward (+)
71690012 .. 71693646
3635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG447200.1

Sequence Viewer

Length: 2103 bp
ATGGATACCCTTTACAGATATTCTGTCGCCCATCCCATGGAGAGGTTCAAATTTGACCATGGCCCAGTTTCAGTCTGTCCAAATCCCAATCTTGTAAATGGGTTCAAAATAAATCATGAATCCTCCAATCCAGTATTACTTGCATCCAAATCTGATCTTTCTAGTGATTCAAGTAATGAGGGTTCAGAGGTAGATGGTCTTGATATCAATGACTGCAACCATCCTGTACTCAAGTACATAAATGATATTCTTCTGGAAGAAGACTTGGAGGGTAAGCCCTGCATGTTGCAGGACTGTTTAGCCCTCCAAGCTGCTGAGAAGTCTTTCTATGATGTCCTTAATCAGAAGGACCCTCCTTTACCCGACCAACCCTCTAGTTCTGTGCACCAAAGCTCTGAGAGTTCAGATGATGATTCCCCACAGAGTTTCCGAAACAGTAACGGCTCTTTTGCTGTTAAGACAGATTGGGTTTGGGATCCGGACTGCTCTCCCGTCCAGTCTTCTCTATTTGAGTCTTCGTCGAATACCCGTTTGGTTTCTGATTCATTGTCTGAGTTGCAAAGTCTTGGGAATTTTGGAGGTGTGGGGGAAGCAAGCAAGTTTCTTTCAAATCCGAAACTTGAAATGTTTGACTTAGAGAGGTTTCAATTGATGCCTCAAGGTTCAGTTGCATCTGAGTCAGGGGATGATGGCTACAACTCAACCAATGTATCGAAGGGAAAGAAGACTCATCAAAGGGAGGATAGTCATTTTCCAGAAGAAGAGAGAAGCAACAAGCAGTCTGCTGCTGTAGGGGACGACTTTGAGCCACAAGAAATGTTTGATGAGGTTTTGCTGTGTCCTGAAAATCATGATGCTAAGCTTTGTGCTGATTATGAGTCTTTGAAAACTGAAGGAGAAGGGAAGTTGCAGCGTAACAAGCAGTCAACAGGATTGAAGGCAAAGCGACAGAGTAACATTGGCGAGTTGGTAGATTTGTGCACAATGCTAACTCAATGTGCACAAGCTGTGGCGAACTTTGACCAACGAACTGCAAGTGAACTACTCAAGCAGATTAGGCAGCACACAACGCCCCATGGTGATGCAACCCAGAGGTTAGCCCATTACTTTGCTGATGGCCTCCACGCGCGGTTGGCTGGTGCCAAAATCCCATCGTATTCGCCTCTTGTAAGTGTGCAGACATCAGCTGCTGAAATCTTAAAAGCATACCAGGTAGTTATTAAAGCATGCCCTTTCAAGAAGATGTCACAATTCTTTGCCAACAGAACAATCATGAAGCTAGCAGAAAAAGCAACAAGGCTTCACATTATTGATTTTGGTATTTCTTATGGATTTCAATGGCCTTGCCTGATCCAACGTCTGTCCCAAAGACCTGGTGGACCTCCTAAGCTTCGTATCACAGCCATTGAGCTTCCCCAACCAGGTTTTCGACCTACAGAAAGAGTTGAAGAGACCGGACGTCGCTTAGAAAAATACGCCAAGAGATTTGATGTCCCATTTGAGTATAATGTCATTGCCCAGAAATGGGAAACTATCCGAATTGAGGACATCAAAATTGATAGAACTGAGCTGATTGTGGTCAATTGTTTGCACCGGTTAAGGAACATACCAGATGAGACGGTGATGGTGAACAGTCCAAGAGATGCTGTCCTTAAGTTGATAAGGAAAATTAGTCCAGACCTTTTCATTCATGGTGTTGTCAATGGGACATACAATGCTCCCTTCTTTCTCACACGATTTAGAGAGGCACTCCTCCACTTCTATTCACTGTTTGATATGCTTGAGGTGACCATCTCTCGTGAAGATGAACAGCGGGTGATGTTTGAACAAGAAATATATGGTAGAGATATACTTAATGTTGTAGCGTGTGAAGGACTGGAAAGAGTTGAAAGGCCTGAAACATACAGGCAGTGGCAGGTCAGGAATTTGAGGGCTGGGTTCAAGCAGCTACCATTAGACCAGAAGCTGTTGAAGGAAGTGAAGACTATGTCAAAAGTAATGCGTTATCATAATGATTTTAGAGTTGATGAAGATGGCCATTGGATGCTGCAGGGATGGAAAGGAAGAACCATCATGGCTCTTTCTTTTTGGAAGCCTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS RVT_2

Protein Analysis

700

Amino Acids

79.56

Weight (kDa)

5.73

Isoelectric Point (pI)

50.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 328 - 698 3.3e-121 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46600 AT3G46600 AT3G46600 AT5G59450
fragaria_vesca FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36690 FvH4_3g36691 FvH4_3g36700 FvH4_3g36711 FvH4_3g36711 FvH4_3g36720 FvH4_3g36730 FvH4_3g36760
malus_domestica MD03G1088900.v1.1 MD03G1089100.v1.1 MD03G1089200.v1.1 MD03G1089300.v1.1 MD11G1097900.v1.1 MD11G1098000.v1.1 MD11G1098100.v1.1 MD11G1098400.v1.1 MD11G1098900.v1.1
prunus_persica Prupe.6G073300_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073400_v2.0.a1 Prupe.6G073500_v2.0.a1 Prupe.6G073600_v2.0.a1 Prupe.6G073700_v2.0.a1 Prupe.6G073800_v2.0.a1 Prupe.I004400_v2.0.a1 Prupe.I004500_v2.0.a1
pyrus_communis pycom03g07050 pycom03g07060 pycom03g07070 pycom03g07110 pycom04g11870 pycom11g08270 pycom11g08280
rosa_chinensis RchiOBHm_Chr5g0065591 RchiOBHm_Chr5g0065601 RchiOBHm_Chr5g0065621 RchiOBHm_Chr5g0065631 RchiOBHm_Chr5g0065641 RchiOBHm_Chr5g0065651 RchiOBHm_Chr5g0065671 RchiOBHm_Chr5g0065721 RchiOBHm_Chr5g0065731 RchiOBHm_Chr5g0065751 RchiOBHm_Chr5g0065791 RchiOBHm_Chr5g0065811
rosa_laevigata RLG00000035800 RLG00000035801 RLG00000035803 RLG00000035804 RLG00000035805 RLG00000035806 RLG00000035807 RLG00000035808 RLG00000035809 RLG00000035810 RLG00000035813 RLG00000035814 RLG00000035816
rosa_multiflora Rmu_co8233911.1_g000001 Rmu_co8258053.1_g000001 Rmu_co8279117.1_g000001 Rmu_sc0000235.1_g000004 Rmu_sc0000235.1_g000024 Rmu_sc0000235.1_g000060 Rmu_sc0000235.1_g000074 Rmu_sc0001010.1_g000001 Rmu_sc0001010.1_g000015 Rmu_sc0001010.1_g000030 Rmu_sc0001010.1_g000037 Rmu_sc0001010.1_g000043 Rmu_sc0001010.1_g000063 Rmu_sc0001010.1_g000066 Rmu_sc0003636.1_g000008 Rmu_sc0003636.1_g000011 Rmu_sc0003636.1_g000012 Rmu_sc0003636.1_g000015 Rmu_sc0005971.1_g000017 Rmu_sc0008231.1_g000001 Rmu_sc0032155.1_g000001
rosa_roxburghii Rroxscaffold_1G00015360 Rroxscaffold_1G00015390 Rroxscaffold_1G00015410 Rroxscaffold_1G00015430 Rroxscaffold_1G00015490 Rroxscaffold_1G00015520 Rroxscaffold_1G00015530 Rroxscaffold_1G00015550 Rroxscaffold_1G00015560 Rroxscaffold_1G00015580 Rroxscaffold_1G00015600 Rroxscaffold_1G00015610 Rroxscaffold_1G00015620 Rroxscaffold_1G00015630
rosa_rugosa Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0372800 Rorug05G0373100 Rorug05G0373100 Rorug05G0373200 Rorug05G0373300 Rorug05G0373400 Rorug05G0373500 Rorug05G0373600 Rorug05G0373700 Rorug05G0378700
rosa_samantha Rh5AG430900 Rh5AG431000 Rh5AG431100 Rh5AG431200 Rh5AG431700 Rh5AG431800 Rh5AG431900 Rh5AG432200 Rh5AG432500 Rh5BG445200 Rh5BG446000 Rh5BG447100 Rh5BG447200 Rh5BG447400 Rh5BG447800 Rh5BG447900 Rh5BG448000 Rh5BG448100 Rh5BG448300 Rh5BG448400 Rh5BG448500 Rh5BG448600 Rh5CG467400 Rh5CG468000 Rh5CG469000 Rh5CG469100 Rh5CG469400 Rh5CG469700 Rh5CG469900 Rh5CG470100 Rh5CG470200 Rh5CG470600 Rh5CG470700 Rh5CG470800 Rh5DG459500 Rh5DG459700 Rh5DG459800 Rh5DG460000 Rh5DG460900 Rh5DG461300 Rh5DG461500 Rh5DG461900 Rh5DG462000 Rh5DG462100
rosa_wichuraiana Rw5G040420 Rw5G040430 Rw5G040440 Rw5G040470 Rw5G040480 Rw5G040500 Rw5G040510 Rw5G040530 Rw5G040550 Rw5G040560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1462
Acc36I ACCTGC 1 cut(s) 1906
AccB1I GGYRCC 1 cut(s) 1139
AccB7I CCANNNNNTGG 1 cut(s) 37
AccII CGCG 2 cut(s) 1127, 1129
AccIII TCCGGA 1 cut(s) 478
AciI CCGC 2 cut(s) 1129, 1813
AclWI GGATC 3 cut(s) 470, 483, 1345
AcoI YGGCCR 1 cut(s) 2035
AcsI RAATTY 3 cut(s) 50, 571, 1924
AcuI CTGAAG 1 cut(s) 912
AcyI GRCGYC 1 cut(s) 1459
AfaI GTAC 2 cut(s) 228, 236
AfiI CCNNNNNNNGG 6 cut(s) 37, 42, 1421, 1524, 1525, 1543
AflII CTTAAG 1 cut(s) 1652
AgeI ACCGGT 1 cut(s) 1593
AhdI GACNNNNNGTC 1 cut(s) 1458
AjnI CCWGG 3 cut(s) 1209, 1372, 1420
AjuI GAANNNNNNNTTGG 4 cut(s) 515, 547, 1410, 1442
Alw21I GWGCWC 3 cut(s) 387, 983, 1003
Alw26I GTCTC 2 cut(s) 1445, 1610
Alw44I GTGCAC 3 cut(s) 383, 979, 999
AlwI GGATC 3 cut(s) 470, 483, 1345
AlwNI CAGNNNCTG 2 cut(s) 1190, 1966
Aor13HI TCCGGA 1 cut(s) 478
AoxI GGCC 5 cut(s) 61, 1117, 1340, 1892, 2035
ApaLI GTGCAC 3 cut(s) 383, 979, 999
ApeKI GCWGC 7 cut(s) 311, 785, 910, 1060, 1187, 1945, 2047
ApoI RAATTY 3 cut(s) 50, 571, 1924
AsiGI ACCGGT 1 cut(s) 1593
Asp700I GAANNNNTTC 1 cut(s) 323
AspLEI GCGC 1 cut(s) 1129
AspS9I GGNCC 3 cut(s) 62, 349, 1379
AsuHPI GGTGA 5 cut(s) 1091, 1633, 1639, 1798, 1828
AsuNHI GCTAGC 1 cut(s) 1279
AvaII GGWCC 2 cut(s) 349, 1379
BaeGI GKGCMC 3 cut(s) 387, 983, 1003
BaeI ACNNNNGTAYC 2 cut(s) 693, 726
BalI TGGCCA 1 cut(s) 2037
BamHI GGATCC 1 cut(s) 475
BanI GGYRCC 1 cut(s) 1139
BauI CACGAG 1 cut(s) 1797
BbsI GAAGAC 5 cut(s) 267, 492, 507, 731, 1988
Bbv12I GWGCWC 3 cut(s) 387, 983, 1003
BbvI GCAGC 7 cut(s) 298, 772, 922, 1072, 1174, 1957, 2034
BceAI ACGGC 1 cut(s) 457
BciT130I CCWGG 3 cut(s) 1211, 1374, 1422
BcoDI GTCTC 2 cut(s) 1445, 1610
BfaI CTAG 4 cut(s) 162, 375, 1280, 2101
BfmI CTRYAG 3 cut(s) 789, 1434, 2048
BfrI CTTAAG 1 cut(s) 1652
BfuAI ACCTGC 1 cut(s) 1906
BisI GCNGC 7 cut(s) 312, 786, 911, 1061, 1188, 1946, 2048
BlpI GCTNAGC 1 cut(s) 858
BlsI GCNGC 7 cut(s) 313, 787, 912, 1062, 1189, 1947, 2049
Bme1390I CCNGG 3 cut(s) 1211, 1374, 1422
Bme18I GGWCC 2 cut(s) 349, 1379
BmeRI GACNNNNNGTC 1 cut(s) 1458
BmgT120I GGNCC 3 cut(s) 62, 349, 1379
BmiI GGNNCC 3 cut(s) 351, 477, 1141
BmrFI CCNGG 3 cut(s) 1211, 1374, 1422
BmrI ACTGGG 1 cut(s) 59
BmsI GCATC 7 cut(s) 152, 642, 680, 844, 1072, 1633, 2034
BmtI GCTAGC 1 cut(s) 1283
BmuI ACTGGG 1 cut(s) 59
BpiI GAAGAC 5 cut(s) 267, 492, 507, 731, 1988
BplI GAGNNNNNCTC 2 cut(s) 1734, 1766
Bpu10I CCTNAGC 1 cut(s) 1386
Bpu1102I GCTNAGC 1 cut(s) 858
BpuEI CTTGAG 4 cut(s) 215, 642, 1031, 1802
BsaHI GRCGYC 1 cut(s) 1459
BsaI GGTCTC 1 cut(s) 1445
BsaJI CCNNGG 3 cut(s) 36, 58, 1075
BsaWI WCCGGW 3 cut(s) 478, 1454, 1593
Bsc4I CCNNNNNNNGG 6 cut(s) 37, 42, 1421, 1524, 1525, 1543
Bse118I RCCGGY 1 cut(s) 1593
Bse1I ACTGG 4 cut(s) 65, 131, 496, 1881
Bse3DI GCAATG 1 cut(s) 1512
BseAI TCCGGA 1 cut(s) 478
BseBI CCWGG 3 cut(s) 1211, 1374, 1422
BseDI CCNNGG 3 cut(s) 36, 58, 1075
BseGI GGATG 6 cut(s) 31, 143, 220, 691, 2049, 2060
BseLI CCNNNNNNNGG 6 cut(s) 37, 42, 1421, 1524, 1525, 1543
BseMI GCAATG 1 cut(s) 1512
BseMII CTCAG 5 cut(s) 306, 387, 543, 666, 1557
BseNI ACTGG 4 cut(s) 65, 131, 496, 1881
BseRI GAGGAG 1 cut(s) 1742
BseSI GKGCMC 3 cut(s) 387, 983, 1003
BseXI GCAGC 7 cut(s) 298, 772, 922, 1072, 1174, 1957, 2034
BseYI CCCAGC 1 cut(s) 1934
BsgI GTGCAG 1 cut(s) 1196
Bsh1236I CGCG 2 cut(s) 1127, 1129
BshFI GGCC 5 cut(s) 63, 1119, 1342, 1894, 2037
BshNI GGYRCC 1 cut(s) 1139
BshTI ACCGGT 1 cut(s) 1593
BsiHKAI GWGCWC 3 cut(s) 387, 983, 1003
BsiSI CCGG 3 cut(s) 479, 1455, 1594
BslFI GGGAC 4 cut(s) 809, 1348, 1478, 1720
BslI CCNNNNNNNGG 6 cut(s) 37, 42, 1421, 1524, 1525, 1543
BsmAI GTCTC 2 cut(s) 1445, 1610
BsmBI CGTCTC 1 cut(s) 1610
BsmFI GGGAC 4 cut(s) 809, 1348, 1478, 1720
BsnI GGCC 5 cut(s) 63, 1119, 1342, 1894, 2037
Bso31I GGTCTC 1 cut(s) 1445
Bsp1286I GDGCHC 3 cut(s) 387, 983, 1003
Bsp13I TCCGGA 1 cut(s) 478
Bsp143I GATC 3 cut(s) 154, 475, 1350
Bsp1720I GCTNAGC 1 cut(s) 858
Bsp19I CCATGG 3 cut(s) 36, 58, 1075
BspACI CCGC 2 cut(s) 1129, 1813
BspANI GGCC 5 cut(s) 63, 1119, 1342, 1894, 2037
BspCNI CTCAG 5 cut(s) 307, 388, 544, 667, 1558
BspEI TCCGGA 1 cut(s) 478
BspFNI CGCG 2 cut(s) 1127, 1129
BspHI TCATGA 3 cut(s) 115, 850, 1272
BspLI GGNNCC 3 cut(s) 351, 477, 1141
BspMAI CTGCAG 1 cut(s) 2052
BspMI ACCTGC 1 cut(s) 1906
BspOI GCTAGC 1 cut(s) 1283
BspPI GGATC 3 cut(s) 470, 483, 1345
BspT107I GGYRCC 1 cut(s) 1139
BspTI CTTAAG 1 cut(s) 1652
BspTNI GGTCTC 1 cut(s) 1445
BsrDI GCAATG 1 cut(s) 1512
BsrFI RCCGGY 1 cut(s) 1593
BsrI ACTGG 4 cut(s) 65, 131, 496, 1881
BssAI RCCGGY 1 cut(s) 1593
BssECI CCNNGG 3 cut(s) 36, 58, 1075
BssMI GATC 3 cut(s) 154, 475, 1350
BssNI GRCGYC 1 cut(s) 1459
BssSI CACGAG 1 cut(s) 1797
BssT1I CCWWGG 3 cut(s) 36, 58, 1075
Bst2BI CACGAG 1 cut(s) 1797
Bst2UI CCWGG 3 cut(s) 1211, 1374, 1422
Bst4CI ACNGT 5 cut(s) 296, 437, 1621, 1634, 1770
Bst6I CTCTTC 2 cut(s) 756, 1443
BstACI GRCGYC 1 cut(s) 1459
BstAFI CTTAAG 1 cut(s) 1652
BstC8I GCNNGC 3 cut(s) 595, 1228, 1281
BstDEI CTNAG 9 cut(s) 315, 396, 552, 634, 675, 858, 1386, 1465, 1566
BstDSI CCRYGG 3 cut(s) 36, 58, 1075
BstEII GGTNACC 1 cut(s) 1786
BstF5I GGATG 6 cut(s) 31, 143, 220, 691, 2049, 2060
BstFNI CGCG 2 cut(s) 1127, 1129
BstHHI GCGC 1 cut(s) 1129
BstKTI GATC 3 cut(s) 157, 478, 1353
BstMAI GTCTC 2 cut(s) 1445, 1610
BstMBI GATC 3 cut(s) 154, 475, 1350
BstMWI GCNNNNNNNGC 6 cut(s) 308, 919, 1057, 1069, 1133, 1289
BstNI CCWGG 3 cut(s) 1211, 1374, 1422
BstNSI RCATGY 2 cut(s) 286, 1230
BstPI GGTNACC 1 cut(s) 1786
BstSCI CCNGG 3 cut(s) 1209, 1372, 1420
BstSFI CTRYAG 3 cut(s) 789, 1434, 2048
BstSLI GKGCMC 3 cut(s) 387, 983, 1003
BstUI CGCG 2 cut(s) 1127, 1129
BstV1I GCAGC 7 cut(s) 298, 772, 922, 1072, 1174, 1957, 2034
BstV2I GAAGAC 5 cut(s) 267, 492, 507, 731, 1988
BstX2I RGATCY 1 cut(s) 475
BstXI CCANNNNNNTGG 1 cut(s) 1373
BstYI RGATCY 1 cut(s) 475
BsuRI GGCC 5 cut(s) 63, 1119, 1342, 1894, 2037
BtgI CCRYGG 3 cut(s) 36, 58, 1075
BtsCI GGATG 6 cut(s) 31, 143, 220, 691, 2049, 2060
BtsI GCAGTG 1 cut(s) 1916
BtsIMutI CAGTG 2 cut(s) 1766, 1916
BveI ACCTGC 1 cut(s) 1906
Cac8I GCNNGC 3 cut(s) 595, 1228, 1281
CaiI CAGNNNCTG 2 cut(s) 1190, 1966
CciI TCATGA 3 cut(s) 115, 850, 1272
CfoI GCGC 1 cut(s) 1129
Cfr10I RCCGGY 1 cut(s) 1593
Cfr13I GGNCC 3 cut(s) 62, 349, 1379
CsiI ACCWGGT 3 cut(s) 1209, 1372, 1420
Csp6I GTAC 2 cut(s) 227, 235
CspAI ACCGGT 1 cut(s) 1593
CviQI GTAC 2 cut(s) 227, 235
DdeI CTNAG 9 cut(s) 315, 396, 552, 634, 675, 858, 1386, 1465, 1566
DpnI GATC 3 cut(s) 156, 477, 1352
DpnII GATC 3 cut(s) 154, 475, 1350
DriI GACNNNNNGTC 1 cut(s) 1458
EaeI YGGCCR 1 cut(s) 2035
Eam1104I CTCTTC 2 cut(s) 756, 1443
Eam1105I GACNNNNNGTC 1 cut(s) 1458
EarI CTCTTC 2 cut(s) 756, 1443
Eco130I CCWWGG 3 cut(s) 36, 58, 1075
Eco147I AGGCCT 1 cut(s) 1894
Eco31I GGTCTC 1 cut(s) 1445
Eco32I GATATC 1 cut(s) 205
Eco47I GGWCC 2 cut(s) 349, 1379
Eco57I CTGAAG 1 cut(s) 912
Eco91I GGTNACC 1 cut(s) 1786
EcoO109I RGGNCCY 1 cut(s) 349
EcoO65I GGTNACC 1 cut(s) 1786
EcoRII CCWGG 3 cut(s) 1209, 1372, 1420
EcoRV GATATC 1 cut(s) 205
EcoT14I CCWWGG 3 cut(s) 36, 58, 1075
ErhI CCWWGG 3 cut(s) 36, 58, 1075
Esp3I CGTCTC 1 cut(s) 1610
FaqI GGGAC 4 cut(s) 809, 1348, 1478, 1720
FauI CCCGC 1 cut(s) 1806
Fnu4HI GCNGC 7 cut(s) 312, 786, 911, 1061, 1188, 1946, 2048
FokI GGATG 6 cut(s) 18, 130, 207, 698, 2056, 2067
Fsp4HI GCNGC 7 cut(s) 312, 786, 911, 1061, 1188, 1946, 2048
FspBI CTAG 4 cut(s) 162, 375, 1280, 2101
GlaI GCGC 1 cut(s) 1128
GluI GCNGC 7 cut(s) 312, 786, 911, 1061, 1188, 1946, 2048
GsaI CCCAGC 1 cut(s) 1938
HaeIII GGCC 5 cut(s) 63, 1119, 1342, 1894, 2037
HapII CCGG 3 cut(s) 479, 1455, 1594
HhaI GCGC 1 cut(s) 1129
Hin1I GRCGYC 1 cut(s) 1459
Hin6I GCGC 1 cut(s) 1127
HinP1I GCGC 1 cut(s) 1127
HincII GTYRAC 1 cut(s) 927
HindII GTYRAC 1 cut(s) 927
HindIII AAGCTT 2 cut(s) 860, 1388
HinfI GANTC 8 cut(s) 119, 167, 413, 512, 542, 677, 727, 878
HpaII CCGG 3 cut(s) 479, 1455, 1594
HphI GGTGA 5 cut(s) 1091, 1633, 1639, 1798, 1828
Hpy166II GTNNAC 7 cut(s) 385, 927, 981, 1001, 1040, 1379, 1630
Hpy8I GTNNAC 7 cut(s) 385, 927, 981, 1001, 1040, 1379, 1630
Hpy99I CGWCG 2 cut(s) 523, 1464
HpyAV CCTTC 8 cut(s) 340, 709, 887, 893, 931, 1732, 1865, 1966
HpyCH4III ACNGT 5 cut(s) 296, 437, 1621, 1634, 1770
HpyCH4IV ACGT 2 cut(s) 1357, 1459
HpyF10VI GCNNNNNNNGC 6 cut(s) 308, 919, 1057, 1069, 1133, 1289
HpyF3I CTNAG 9 cut(s) 315, 396, 552, 634, 675, 858, 1386, 1465, 1566
HpySE526I ACGT 2 cut(s) 1357, 1459
Hsp92I GRCGYC 1 cut(s) 1459
HspAI GCGC 1 cut(s) 1127
Kpn2I TCCGGA 1 cut(s) 478
Kzo9I GATC 3 cut(s) 154, 475, 1350
LmnI GCTCC 1 cut(s) 1723
Lsp1109I GCAGC 7 cut(s) 298, 772, 922, 1072, 1174, 1957, 2034
LweI GCATC 7 cut(s) 152, 642, 680, 844, 1072, 1633, 2034
MabI ACCWGGT 3 cut(s) 1209, 1372, 1420
MaeI CTAG 4 cut(s) 162, 375, 1280, 2101
MaeII ACGT 2 cut(s) 1357, 1459
MaeIII GTNAC 5 cut(s) 437, 914, 953, 1245, 1786
MalI GATC 3 cut(s) 156, 477, 1352
MboI GATC 3 cut(s) 154, 475, 1350
MfeI CAATTG 2 cut(s) 647, 1582
MflI RGATCY 1 cut(s) 475
MhlI GDGCHC 3 cut(s) 387, 983, 1003
MlsI TGGCCA 1 cut(s) 2037
MluCI AATT 9 cut(s) 50, 571, 647, 1250, 1539, 1554, 1582, 1668, 1924
MluNI TGGCCA 1 cut(s) 2037
MlyI GAGTC 4 cut(s) 521, 686, 721, 887
MmeI TCCRAC 1 cut(s) 1378
Mox20I TGGCCA 1 cut(s) 2037
MroI TCCGGA 1 cut(s) 478
MroXI GAANNNNTTC 1 cut(s) 323
MscI TGGCCA 1 cut(s) 2037
MseI TTAA 7 cut(s) 339, 456, 1199, 1221, 1598, 1653, 1854
MslI CAYNNNNRTG 1 cut(s) 1080
Msp20I TGGCCA 1 cut(s) 2037
MspA1I CMGCKG 2 cut(s) 1187, 1813
MspCI CTTAAG 1 cut(s) 1652
MspI CCGG 3 cut(s) 479, 1455, 1594
MspR9I CCNGG 3 cut(s) 1211, 1374, 1422
MunI CAATTG 2 cut(s) 647, 1582
MvaI CCWGG 3 cut(s) 1211, 1374, 1422
MvnI CGCG 2 cut(s) 1127, 1129
MwoI GCNNNNNNNGC 6 cut(s) 308, 919, 1057, 1069, 1133, 1289
NcoI CCATGG 3 cut(s) 36, 58, 1075
NdeII GATC 3 cut(s) 154, 475, 1350
NheI GCTAGC 1 cut(s) 1279
NlaIV GGNNCC 3 cut(s) 351, 477, 1141
NmuCI GTSAC 2 cut(s) 1245, 1786
NspI RCATGY 2 cut(s) 286, 1230
PaeI GCATGC 1 cut(s) 1230
PagI TCATGA 3 cut(s) 115, 850, 1272
PceI AGGCCT 1 cut(s) 1894
PdmI GAANNNNTTC 1 cut(s) 323
PfeI GAWTC 4 cut(s) 119, 167, 413, 542
PflFI GACNNNGTC 1 cut(s) 1987
PflMI CCANNNNNTGG 1 cut(s) 37
PinAI ACCGGT 1 cut(s) 1593
PkrI GCNGC 7 cut(s) 313, 787, 912, 1062, 1189, 1947, 2049
PleI GAGTC 4 cut(s) 520, 685, 721, 886
PpsI GAGTC 4 cut(s) 520, 685, 721, 886
PpuMI RGGWCCY 1 cut(s) 349
Psp5II RGGWCCY 1 cut(s) 349
Psp6I CCWGG 3 cut(s) 1209, 1372, 1420
PspEI GGTNACC 1 cut(s) 1786
PspFI CCCAGC 1 cut(s) 1934
PspGI CCWGG 3 cut(s) 1209, 1372, 1420
PspN4I GGNNCC 3 cut(s) 351, 477, 1141
PspPI GGNCC 3 cut(s) 62, 349, 1379
PspPPI RGGWCCY 1 cut(s) 349
PsrI GAACNNNNNNTAC 2 cut(s) 166, 198
PstI CTGCAG 1 cut(s) 2052
PstNI CAGNNNCTG 2 cut(s) 1190, 1966
PsuI RGATCY 1 cut(s) 475
PsyI GACNNNGTC 1 cut(s) 1987
PvuII CAGCTG 1 cut(s) 1187
RsaI GTAC 2 cut(s) 228, 236
RsaNI GTAC 2 cut(s) 227, 235
RseI CAYNNNNRTG 1 cut(s) 1080
SaqAI TTAA 7 cut(s) 339, 456, 1199, 1221, 1598, 1653, 1854
SatI GCNGC 7 cut(s) 312, 786, 911, 1061, 1188, 1946, 2048
Sau3AI GATC 3 cut(s) 154, 475, 1350
Sau96I GGNCC 3 cut(s) 62, 349, 1379
SchI GAGTC 4 cut(s) 521, 686, 721, 887
ScrFI CCNGG 3 cut(s) 1211, 1374, 1422
SduI GDGCHC 3 cut(s) 387, 983, 1003
SexAI ACCWGGT 3 cut(s) 1209, 1372, 1420
SfaNI GCATC 7 cut(s) 152, 642, 680, 844, 1072, 1633, 2034
SfcI CTRYAG 3 cut(s) 789, 1434, 2048
SinI GGWCC 2 cut(s) 349, 1379
SmiMI CAYNNNNRTG 1 cut(s) 1080
SmlI CTYRAG 5 cut(s) 230, 657, 1046, 1652, 1781
SmoI CTYRAG 5 cut(s) 230, 657, 1046, 1652, 1781
SphI GCATGC 1 cut(s) 1230
Sse9I AATT 9 cut(s) 50, 571, 647, 1250, 1539, 1554, 1582, 1668, 1924
SseBI AGGCCT 1 cut(s) 1894
SsiI CCGC 2 cut(s) 1129, 1813
SspMI CTAG 4 cut(s) 162, 375, 1280, 2101
StuI AGGCCT 1 cut(s) 1894
StyD4I CCNGG 3 cut(s) 1209, 1372, 1420
StyI CCWWGG 3 cut(s) 36, 58, 1075
TaaI ACNGT 5 cut(s) 296, 437, 1621, 1634, 1770
TaiI ACGT 2 cut(s) 1360, 1462
TaqI TCGA 3 cut(s) 521, 713, 1429
TasI AATT 9 cut(s) 50, 571, 647, 1250, 1539, 1554, 1582, 1668, 1924
TatI WGTACW 2 cut(s) 226, 234
TfiI GAWTC 4 cut(s) 119, 167, 413, 542
Tru1I TTAA 7 cut(s) 339, 456, 1199, 1221, 1598, 1653, 1854
Tru9I TTAA 7 cut(s) 339, 456, 1199, 1221, 1598, 1653, 1854
TscAI CASTG 2 cut(s) 1773, 1916
TseFI GTSAC 2 cut(s) 1245, 1786
TseI GCWGC 7 cut(s) 311, 785, 910, 1060, 1187, 1945, 2047
Tsp45I GTSAC 2 cut(s) 1245, 1786
TspDTI ATGAA 7 cut(s) 132, 534, 1289, 1675, 1679, 1821, 2043
TspRI CASTG 2 cut(s) 1773, 1916
Tth111I GACNNNGTC 1 cut(s) 1987
Van91I CCANNNNNTGG 1 cut(s) 37
Vha464I CTTAAG 1 cut(s) 1652
VneI GTGCAC 3 cut(s) 383, 979, 999
VpaK11BI GGWCC 2 cut(s) 349, 1379
XapI RAATTY 3 cut(s) 50, 571, 1924
XceI RCATGY 2 cut(s) 286, 1230
XcmI CCANNNNNNNNNTGG 1 cut(s) 1373
XmnI GAANNNNTTC 1 cut(s) 323
XspI CTAG 4 cut(s) 162, 375, 1280, 2101
ZraI GACGTC 1 cut(s) 1460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.