Reverse transcriptase (RNA-dependent DNA polymerase)
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| GRAS | PF03514 | 326 - 696 | 9.1e-114 | GRAS domain family |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 191 |
| AbsI | CCTCGAGG | 1 cut(s) | 1115 |
| Acc36I | ACCTGC | 1 cut(s) | 1900 |
| Acc65I | GGTACC | 1 cut(s) | 621 |
| AccB1I | GGYRCC | 1 cut(s) | 621 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 89 |
| AccI | GTMKAC | 1 cut(s) | 1582 |
| AccII | CGCG | 3 cut(s) | 614, 995, 2091 |
| AciI | CCGC | 2 cut(s) | 614, 812 |
| AclWI | GGATC | 4 cut(s) | 452, 667, 925, 1806 |
| AcsI | RAATTY | 5 cut(s) | 21, 556, 595, 664, 1674 |
| AcuI | CTGAAG | 3 cut(s) | 447, 1061, 1529 |
| AcyI | GRCGYC | 1 cut(s) | 188 |
| AfaI | GTAC | 6 cut(s) | 172, 366, 623, 695, 1122, 1315 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 89, 1415, 1434 |
| AflIII | ACRYGT | 1 cut(s) | 1727 |
| AgeI | ACCGGT | 1 cut(s) | 1587 |
| AjnI | CCWGG | 2 cut(s) | 1414, 1953 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 618, 650, 1404, 1436 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 12, 44 |
| Alw21I | GWGCWC | 1 cut(s) | 977 |
| Alw26I | GTCTC | 5 cut(s) | 934, 1357, 1629, 1833, 1886 |
| Alw44I | GTGCAC | 1 cut(s) | 973 |
| AlwI | GGATC | 4 cut(s) | 452, 667, 925, 1806 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 674, 1960 |
| Ama87I | CYCGRG | 1 cut(s) | 1115 |
| AoxI | GGCC | 5 cut(s) | 306, 1111, 1334, 1779, 1886 |
| ApaLI | GTGCAC | 1 cut(s) | 973 |
| ApeKI | GCWGC | 4 cut(s) | 317, 541, 1939, 2041 |
| ApoI | RAATTY | 5 cut(s) | 21, 556, 595, 664, 1674 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 656, 688 |
| AseI | ATTAAT | 1 cut(s) | 629 |
| AsiGI | ACCGGT | 1 cut(s) | 1587 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 329 |
| Asp718I | GGTACC | 1 cut(s) | 621 |
| AspLEI | GCGC | 2 cut(s) | 995, 997 |
| AspS9I | GGNCC | 5 cut(s) | 151, 307, 641, 1335, 1373 |
| AsuHPI | GGTGA | 5 cut(s) | 218, 467, 1085, 1704, 1984 |
| AsuII | TTCGAA | 2 cut(s) | 600, 1818 |
| AvaI | CYCGRG | 1 cut(s) | 1115 |
| AvaII | GGWCC | 3 cut(s) | 151, 641, 1373 |
| BaeGI | GKGCMC | 1 cut(s) | 977 |
| BanI | GGYRCC | 1 cut(s) | 621 |
| BanII | GRGCYC | 1 cut(s) | 62 |
| BbsI | GAAGAC | 5 cut(s) | 477, 1238, 1286, 1982, 2031 |
| Bbv12I | GWGCWC | 1 cut(s) | 977 |
| BbvI | GCAGC | 4 cut(s) | 304, 528, 1951, 2028 |
| BccI | CCATC | 3 cut(s) | 1103, 1612, 2043 |
| BciT130I | CCWGG | 2 cut(s) | 1416, 1955 |
| BciVI | GTATCC | 1 cut(s) | 502 |
| BclI | TGATCA | 2 cut(s) | 52, 1653 |
| BcoDI | GTCTC | 5 cut(s) | 934, 1357, 1629, 1833, 1886 |
| BfaI | CTAG | 4 cut(s) | 168, 755, 1137, 1274 |
| BfmI | CTRYAG | 2 cut(s) | 360, 2042 |
| BfuAI | ACCTGC | 1 cut(s) | 1900 |
| BfuI | GTATCC | 1 cut(s) | 502 |
| BisI | GCNGC | 5 cut(s) | 318, 542, 812, 1940, 2042 |
| BlpI | GCTNAGC | 2 cut(s) | 1126, 1459 |
| BlsI | GCNGC | 5 cut(s) | 319, 543, 813, 1941, 2043 |
| BmcAI | AGTACT | 1 cut(s) | 366 |
| Bme1390I | CCNGG | 2 cut(s) | 1416, 1955 |
| Bme18I | GGWCC | 3 cut(s) | 151, 641, 1373 |
| BmeT110I | CYCGRG | 1 cut(s) | 1115 |
| BmgT120I | GGNCC | 5 cut(s) | 151, 307, 641, 1335, 1373 |
| BmiI | GGNNCC | 1 cut(s) | 623 |
| BmrFI | CCNGG | 2 cut(s) | 1416, 1955 |
| BmsI | GCATC | 5 cut(s) | 346, 733, 1066, 1747, 2028 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1211 |
| BpiI | GAAGAC | 5 cut(s) | 477, 1238, 1286, 1982, 2031 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1078, 1110 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1380 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1126, 1459 |
| Bpu14I | TTCGAA | 2 cut(s) | 600, 1818 |
| BpuEI | CTTGAG | 1 cut(s) | 221 |
| BsaHI | GRCGYC | 1 cut(s) | 188 |
| BsaJI | CCNNGG | 2 cut(s) | 516, 1992 |
| BsaWI | WCCGGW | 1 cut(s) | 1587 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 89, 1415, 1434 |
| Bse118I | RCCGGY | 1 cut(s) | 1587 |
| Bse1I | ACTGG | 3 cut(s) | 481, 1453, 1875 |
| Bse3DI | GCAATG | 3 cut(s) | 424, 833, 1506 |
| BseBI | CCWGG | 2 cut(s) | 1416, 1955 |
| BseDI | CCNNGG | 2 cut(s) | 516, 1992 |
| BseGI | GGATG | 6 cut(s) | 361, 397, 748, 1344, 2043, 2054 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 89, 1415, 1434 |
| BseMI | GCAATG | 3 cut(s) | 424, 833, 1506 |
| BseMII | CTCAG | 7 cut(s) | 312, 659, 666, 714, 798, 1526, 1881 |
| BseNI | ACTGG | 3 cut(s) | 481, 1453, 1875 |
| BsePI | GCGCGC | 1 cut(s) | 993 |
| BseSI | GKGCMC | 1 cut(s) | 977 |
| BseXI | GCAGC | 4 cut(s) | 304, 528, 1951, 2028 |
| BseYI | CCCAGC | 1 cut(s) | 1928 |
| Bsh1236I | CGCG | 3 cut(s) | 614, 995, 2091 |
| BshFI | GGCC | 5 cut(s) | 308, 1113, 1336, 1781, 1888 |
| BshNI | GGYRCC | 1 cut(s) | 621 |
| BshTI | ACCGGT | 1 cut(s) | 1587 |
| BsiHKAI | GWGCWC | 1 cut(s) | 977 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1115 |
| BsiSI | CCGG | 1 cut(s) | 1588 |
| BslFI | GGGAC | 1 cut(s) | 1881 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 89, 1415, 1434 |
| BsmAI | GTCTC | 5 cut(s) | 934, 1357, 1629, 1833, 1886 |
| BsmBI | CGTCTC | 1 cut(s) | 1629 |
| BsmFI | GGGAC | 1 cut(s) | 1881 |
| BsnI | GGCC | 5 cut(s) | 308, 1113, 1336, 1781, 1888 |
| BsoBI | CYCGRG | 1 cut(s) | 1115 |
| Bsp119I | TTCGAA | 2 cut(s) | 600, 1818 |
| Bsp1286I | GDGCHC | 2 cut(s) | 62, 977 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1126, 1459 |
| Bsp19I | CCATGG | 1 cut(s) | 1992 |
| BspACI | CCGC | 2 cut(s) | 614, 812 |
| BspANI | GGCC | 5 cut(s) | 308, 1113, 1336, 1781, 1888 |
| BspCNI | CTCAG | 7 cut(s) | 313, 658, 667, 713, 799, 1525, 1882 |
| BspFNI | CGCG | 3 cut(s) | 614, 995, 2091 |
| BspHI | TCATGA | 2 cut(s) | 112, 880 |
| BspLI | GGNNCC | 1 cut(s) | 623 |
| BspMAI | CTGCAG | 1 cut(s) | 2046 |
| BspMI | ACCTGC | 1 cut(s) | 1900 |
| BspPI | GGATC | 4 cut(s) | 452, 667, 925, 1806 |
| BspT104I | TTCGAA | 2 cut(s) | 600, 1818 |
| BspT107I | GGYRCC | 1 cut(s) | 621 |
| BsrDI | GCAATG | 3 cut(s) | 424, 833, 1506 |
| BsrFI | RCCGGY | 1 cut(s) | 1587 |
| BsrI | ACTGG | 3 cut(s) | 481, 1453, 1875 |
| BssAI | RCCGGY | 1 cut(s) | 1587 |
| BssECI | CCNNGG | 2 cut(s) | 516, 1992 |
| BssHII | GCGCGC | 1 cut(s) | 993 |
| BssNI | GRCGYC | 1 cut(s) | 188 |
| BssT1I | CCWWGG | 2 cut(s) | 516, 1992 |
| Bst2UI | CCWGG | 2 cut(s) | 1416, 1955 |
| Bst6I | CTCTTC | 4 cut(s) | 258, 1066, 1751, 2053 |
| BstACI | GRCGYC | 1 cut(s) | 188 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 794, 2038 |
| BstBI | TTCGAA | 2 cut(s) | 600, 1818 |
| BstC8I | GCNNGC | 6 cut(s) | 927, 995, 999, 1132, 1165, 1169 |
| BstDSI | CCRYGG | 1 cut(s) | 1992 |
| BstF5I | GGATG | 6 cut(s) | 361, 397, 748, 1344, 2043, 2054 |
| BstFNI | CGCG | 3 cut(s) | 614, 995, 2091 |
| BstHHI | GCGC | 2 cut(s) | 995, 997 |
| BstMAI | GTCTC | 5 cut(s) | 934, 1357, 1629, 1833, 1886 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 314, 423, 794, 1131, 1283, 2038 |
| BstNI | CCWGG | 2 cut(s) | 1416, 1955 |
| BstNSI | RCATGY | 1 cut(s) | 1171 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1211 |
| BstSCI | CCNGG | 2 cut(s) | 1414, 1953 |
| BstSFI | CTRYAG | 2 cut(s) | 360, 2042 |
| BstSLI | GKGCMC | 1 cut(s) | 977 |
| BstUI | CGCG | 3 cut(s) | 614, 995, 2091 |
| BstV1I | GCAGC | 4 cut(s) | 304, 528, 1951, 2028 |
| BstV2I | GAAGAC | 5 cut(s) | 477, 1238, 1286, 1982, 2031 |
| BstX2I | RGATCY | 1 cut(s) | 917 |
| BstYI | RGATCY | 1 cut(s) | 917 |
| BsuI | GTATCC | 1 cut(s) | 502 |
| BsuRI | GGCC | 5 cut(s) | 308, 1113, 1336, 1781, 1888 |
| BtgI | CCRYGG | 1 cut(s) | 1992 |
| BtsCI | GGATG | 6 cut(s) | 361, 397, 748, 1344, 2043, 2054 |
| BtsI | GCAGTG | 1 cut(s) | 2039 |
| BtsIMutI | CAGTG | 3 cut(s) | 675, 914, 2039 |
| BveI | ACCTGC | 1 cut(s) | 1900 |
| Cac8I | GCNNGC | 6 cut(s) | 927, 995, 999, 1132, 1165, 1169 |
| CaiI | CAGNNNCTG | 2 cut(s) | 674, 1960 |
| CciI | TCATGA | 2 cut(s) | 112, 880 |
| CfoI | GCGC | 2 cut(s) | 995, 997 |
| Cfr10I | RCCGGY | 1 cut(s) | 1587 |
| Cfr13I | GGNCC | 5 cut(s) | 151, 307, 641, 1335, 1373 |
| CseI | GACGC | 2 cut(s) | 620, 1646 |
| Csp6I | GTAC | 6 cut(s) | 171, 365, 622, 694, 1121, 1314 |
| CspAI | ACCGGT | 1 cut(s) | 1587 |
| CviQI | GTAC | 6 cut(s) | 171, 365, 622, 694, 1121, 1314 |
| Eam1104I | CTCTTC | 4 cut(s) | 258, 1066, 1751, 2053 |
| EarI | CTCTTC | 4 cut(s) | 258, 1066, 1751, 2053 |
| Eco130I | CCWWGG | 2 cut(s) | 516, 1992 |
| Eco147I | AGGCCT | 1 cut(s) | 1888 |
| Eco24I | GRGCYC | 1 cut(s) | 62 |
| Eco32I | GATATC | 1 cut(s) | 1176 |
| Eco47I | GGWCC | 3 cut(s) | 151, 641, 1373 |
| Eco57I | CTGAAG | 3 cut(s) | 447, 1061, 1529 |
| Eco88I | CYCGRG | 1 cut(s) | 1115 |
| EcoO109I | RGGNCCY | 1 cut(s) | 641 |
| EcoRII | CCWGG | 2 cut(s) | 1414, 1953 |
| EcoRV | GATATC | 1 cut(s) | 1176 |
| EcoT14I | CCWWGG | 2 cut(s) | 516, 1992 |
| EcoT38I | GRGCYC | 1 cut(s) | 62 |
| ErhI | CCWWGG | 2 cut(s) | 516, 1992 |
| Esp3I | CGTCTC | 1 cut(s) | 1629 |
| FaqI | GGGAC | 1 cut(s) | 1881 |
| FauNDI | CATATG | 1 cut(s) | 445 |
| FbaI | TGATCA | 2 cut(s) | 52, 1653 |
| FblI | GTMKAC | 1 cut(s) | 1582 |
| Fnu4HI | GCNGC | 5 cut(s) | 318, 542, 812, 1940, 2042 |
| FokI | GGATG | 6 cut(s) | 368, 404, 755, 1331, 2050, 2061 |
| FriOI | GRGCYC | 1 cut(s) | 62 |
| Fsp4HI | GCNGC | 5 cut(s) | 318, 542, 812, 1940, 2042 |
| FspBI | CTAG | 4 cut(s) | 168, 755, 1137, 1274 |
| GlaI | GCGC | 2 cut(s) | 994, 996 |
| GluI | GCNGC | 5 cut(s) | 318, 542, 812, 1940, 2042 |
| GsaI | CCCAGC | 1 cut(s) | 1932 |
| HaeIII | GGCC | 5 cut(s) | 308, 1113, 1336, 1781, 1888 |
| HapII | CCGG | 1 cut(s) | 1588 |
| HgaI | GACGC | 2 cut(s) | 620, 1646 |
| HhaI | GCGC | 2 cut(s) | 995, 997 |
| Hin1I | GRCGYC | 1 cut(s) | 188 |
| Hin6I | GCGC | 2 cut(s) | 993, 995 |
| HinP1I | GCGC | 2 cut(s) | 993, 995 |
| HincII | GTYRAC | 2 cut(s) | 984, 1576 |
| HindII | GTYRAC | 2 cut(s) | 984, 1576 |
| HindIII | AAGCTT | 2 cut(s) | 376, 1382 |
| HinfI | GANTC | 8 cut(s) | 38, 164, 467, 527, 803, 853, 860, 2061 |
| HpaI | GTTAAC | 1 cut(s) | 984 |
| HpaII | CCGG | 1 cut(s) | 1588 |
| HphI | GGTGA | 5 cut(s) | 218, 467, 1085, 1704, 1984 |
| Hpy166II | GTNNAC | 6 cut(s) | 905, 975, 984, 1373, 1576, 1583 |
| Hpy8I | GTNNAC | 6 cut(s) | 905, 975, 984, 1373, 1576, 1583 |
| Hpy99I | CGWCG | 3 cut(s) | 190, 1150, 1458 |
| HpyAV | CCTTC | 7 cut(s) | 146, 164, 471, 558, 1726, 1963, 2089 |
| HpyCH4IV | ACGT | 5 cut(s) | 188, 931, 1208, 1599, 1729 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 314, 423, 794, 1131, 1283, 2038 |
| HpySE526I | ACGT | 5 cut(s) | 188, 931, 1208, 1599, 1729 |
| Hsp92I | GRCGYC | 1 cut(s) | 188 |
| HspAI | GCGC | 2 cut(s) | 993, 995 |
| KpnI | GGTACC | 1 cut(s) | 625 |
| Ksp22I | TGATCA | 2 cut(s) | 52, 1653 |
| KspAI | GTTAAC | 1 cut(s) | 984 |
| LmnI | GCTCC | 2 cut(s) | 1947, 1957 |
| Lsp1109I | GCAGC | 4 cut(s) | 304, 528, 1951, 2028 |
| LweI | GCATC | 5 cut(s) | 346, 733, 1066, 1747, 2028 |
| MaeI | CTAG | 4 cut(s) | 168, 755, 1137, 1274 |
| MaeII | ACGT | 5 cut(s) | 188, 931, 1208, 1599, 1729 |
| MaeIII | GTNAC | 6 cut(s) | 206, 215, 291, 1199, 1213, 1609 |
| MfeI | CAATTG | 1 cut(s) | 1398 |
| MflI | RGATCY | 1 cut(s) | 917 |
| MhlI | GDGCHC | 2 cut(s) | 62, 977 |
| MlyI | GAGTC | 2 cut(s) | 797, 2070 |
| MmeI | TCCRAC | 1 cut(s) | 1510 |
| MroXI | GAANNNNTTC | 1 cut(s) | 329 |
| MseI | TTAA | 7 cut(s) | 15, 345, 629, 891, 983, 1592, 1767 |
| MslI | CAYNNNNRTG | 2 cut(s) | 93, 1755 |
| MspI | CCGG | 1 cut(s) | 1588 |
| MspR9I | CCNGG | 2 cut(s) | 1416, 1955 |
| MunI | CAATTG | 1 cut(s) | 1398 |
| MvaI | CCWGG | 2 cut(s) | 1416, 1955 |
| MvnI | CGCG | 3 cut(s) | 614, 995, 2091 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 314, 423, 794, 1131, 1283, 2038 |
| NcoI | CCATGG | 1 cut(s) | 1992 |
| NdeI | CATATG | 1 cut(s) | 445 |
| NlaIV | GGNNCC | 1 cut(s) | 623 |
| NmuCI | GTSAC | 4 cut(s) | 206, 215, 1213, 1609 |
| NspI | RCATGY | 1 cut(s) | 1171 |
| NspV | TTCGAA | 2 cut(s) | 600, 1818 |
| PaeI | GCATGC | 1 cut(s) | 1171 |
| PaeR7I | CTCGAG | 1 cut(s) | 1115 |
| PagI | TCATGA | 2 cut(s) | 112, 880 |
| PauI | GCGCGC | 1 cut(s) | 993 |
| PceI | AGGCCT | 1 cut(s) | 1888 |
| PdmI | GAANNNNTTC | 1 cut(s) | 329 |
| PfeI | GAWTC | 6 cut(s) | 38, 164, 467, 527, 853, 860 |
| PflFI | GACNNNGTC | 2 cut(s) | 1640, 1981 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 89 |
| PinAI | ACCGGT | 1 cut(s) | 1587 |
| PkrI | GCNGC | 5 cut(s) | 319, 543, 813, 1941, 2043 |
| PleI | GAGTC | 2 cut(s) | 797, 2069 |
| PpsI | GAGTC | 2 cut(s) | 797, 2069 |
| PpuMI | RGGWCCY | 1 cut(s) | 641 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1211 |
| PshBI | ATTAAT | 1 cut(s) | 629 |
| Psp5II | RGGWCCY | 1 cut(s) | 641 |
| Psp6I | CCWGG | 2 cut(s) | 1414, 1953 |
| PspFI | CCCAGC | 1 cut(s) | 1928 |
| PspGI | CCWGG | 2 cut(s) | 1414, 1953 |
| PspN4I | GGNNCC | 1 cut(s) | 623 |
| PspPI | GGNCC | 5 cut(s) | 151, 307, 641, 1335, 1373 |
| PspPPI | RGGWCCY | 1 cut(s) | 641 |
| PspXI | VCTCGAGB | 1 cut(s) | 1115 |
| PstI | CTGCAG | 1 cut(s) | 2046 |
| PstNI | CAGNNNCTG | 2 cut(s) | 674, 1960 |
| PsuI | RGATCY | 1 cut(s) | 917 |
| PsyI | GACNNNGTC | 2 cut(s) | 1640, 1981 |
| PteI | GCGCGC | 1 cut(s) | 993 |
| RsaI | GTAC | 6 cut(s) | 172, 366, 623, 695, 1122, 1315 |
| RsaNI | GTAC | 6 cut(s) | 171, 365, 622, 694, 1121, 1314 |
| RseI | CAYNNNNRTG | 2 cut(s) | 93, 1755 |
| SaqAI | TTAA | 7 cut(s) | 15, 345, 629, 891, 983, 1592, 1767 |
| SatI | GCNGC | 5 cut(s) | 318, 542, 812, 1940, 2042 |
| Sau96I | GGNCC | 5 cut(s) | 151, 307, 641, 1335, 1373 |
| ScaI | AGTACT | 1 cut(s) | 366 |
| SchI | GAGTC | 2 cut(s) | 797, 2070 |
| ScrFI | CCNGG | 2 cut(s) | 1416, 1955 |
| SduI | GDGCHC | 2 cut(s) | 62, 977 |
| SfaNI | GCATC | 5 cut(s) | 346, 733, 1066, 1747, 2028 |
| SfcI | CTRYAG | 2 cut(s) | 360, 2042 |
| Sfr274I | CTCGAG | 1 cut(s) | 1115 |
| SfuI | TTCGAA | 2 cut(s) | 600, 1818 |
| SinI | GGWCC | 3 cut(s) | 151, 641, 1373 |
| SlaI | CTCGAG | 1 cut(s) | 1115 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 93, 1755 |
| SmlI | CTYRAG | 2 cut(s) | 236, 1115 |
| SmoI | CTYRAG | 2 cut(s) | 236, 1115 |
| SphI | GCATGC | 1 cut(s) | 1171 |
| SseBI | AGGCCT | 1 cut(s) | 1888 |
| SsiI | CCGC | 2 cut(s) | 614, 812 |
| SspI | AATATT | 1 cut(s) | 1191 |
| SspMI | CTAG | 4 cut(s) | 168, 755, 1137, 1274 |
| StuI | AGGCCT | 1 cut(s) | 1888 |
| StyD4I | CCNGG | 2 cut(s) | 1414, 1953 |
| StyI | CCWWGG | 2 cut(s) | 516, 1992 |
| TaiI | ACGT | 5 cut(s) | 191, 934, 1211, 1602, 1732 |
| TaqI | TCGA | 7 cut(s) | 261, 600, 1116, 1423, 1551, 1818, 1985 |
| TatI | WGTACW | 2 cut(s) | 170, 364 |
| TauI | GCSGC | 1 cut(s) | 814 |
| TfiI | GAWTC | 6 cut(s) | 38, 164, 467, 527, 853, 860 |
| Tru1I | TTAA | 7 cut(s) | 15, 345, 629, 891, 983, 1592, 1767 |
| Tru9I | TTAA | 7 cut(s) | 15, 345, 629, 891, 983, 1592, 1767 |
| TscAI | CASTG | 3 cut(s) | 675, 914, 2039 |
| TseFI | GTSAC | 4 cut(s) | 206, 215, 1213, 1609 |
| TseI | GCWGC | 4 cut(s) | 317, 541, 1939, 2041 |
| Tsp45I | GTSAC | 4 cut(s) | 206, 215, 1213, 1609 |
| TspDTI | ATGAA | 7 cut(s) | 129, 606, 1283, 1669, 1673, 1862, 2037 |
| TspRI | CASTG | 3 cut(s) | 675, 914, 2039 |
| Tth111I | GACNNNGTC | 2 cut(s) | 1640, 1981 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 89 |
| VneI | GTGCAC | 1 cut(s) | 973 |
| VpaK11BI | GGWCC | 3 cut(s) | 151, 641, 1373 |
| VspI | ATTAAT | 1 cut(s) | 629 |
| XapI | RAATTY | 5 cut(s) | 21, 556, 595, 664, 1674 |
| XbaI | TCTAGA | 1 cut(s) | 754 |
| XceI | RCATGY | 1 cut(s) | 1171 |
| XhoI | CTCGAG | 1 cut(s) | 1115 |
| XmiI | GTMKAC | 1 cut(s) | 1582 |
| XmnI | GAANNNNTTC | 1 cut(s) | 329 |
| XspI | CTAG | 4 cut(s) | 168, 755, 1137, 1274 |
| ZraI | GACGTC | 1 cut(s) | 189 |
| ZrmI | AGTACT | 1 cut(s) | 366 |