Belongs to the GRAS family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1957 |
| AccB1I | GGYRCC | 1 cut(s) | 1509 |
| AccI | GTMKAC | 1 cut(s) | 1225 |
| AccII | CGCG | 2 cut(s) | 988, 1851 |
| AciI | CCGC | 3 cut(s) | 1513, 1864, 1883 |
| AclWI | GGATC | 3 cut(s) | 350, 363, 1605 |
| AcoI | YGGCCR | 3 cut(s) | 52, 1677, 2086 |
| AcsI | RAATTY | 4 cut(s) | 41, 595, 640, 706 |
| AcuI | CTGAAG | 3 cut(s) | 471, 501, 561 |
| AcyI | GRCGYC | 1 cut(s) | 1510 |
| AfaI | GTAC | 5 cut(s) | 172, 242, 668, 1556, 1736 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 1472, 1594 |
| AgeI | ACCGGT | 1 cut(s) | 1644 |
| AjnI | CCWGG | 4 cut(s) | 270, 1423, 1471, 2010 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 26, 58, 116, 148, 1461, 1493 |
| Alw21I | GWGCWC | 4 cut(s) | 185, 237, 1054, 2019 |
| Alw26I | GTCTC | 6 cut(s) | 34, 558, 1414, 1415, 1496, 1943 |
| Alw44I | GTGCAC | 1 cut(s) | 1050 |
| AlwI | GGATC | 3 cut(s) | 350, 363, 1605 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1241 |
| AoxI | GGCC | 8 cut(s) | 52, 306, 1168, 1391, 1677, 1836, 1943, 2086 |
| ApaLI | GTGCAC | 1 cut(s) | 1050 |
| ApeKI | GCWGC | 5 cut(s) | 317, 827, 1238, 1996, 2098 |
| ApoI | RAATTY | 4 cut(s) | 41, 595, 640, 706 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 461, 493 |
| AseI | ATTAAT | 1 cut(s) | 1722 |
| AsiGI | ACCGGT | 1 cut(s) | 1644 |
| AspLEI | GCGC | 2 cut(s) | 988, 1512 |
| AspS9I | GGNCC | 4 cut(s) | 307, 686, 1392, 1430 |
| AsuHPI | GGTGA | 6 cut(s) | 357, 800, 1142, 1203, 1761, 2059 |
| AvaII | GGWCC | 2 cut(s) | 686, 1430 |
| BaeGI | GKGCMC | 1 cut(s) | 1054 |
| BalI | TGGCCA | 3 cut(s) | 54, 1679, 2088 |
| BamHI | GGATCC | 1 cut(s) | 355 |
| BanI | GGYRCC | 1 cut(s) | 1509 |
| BanII | GRGCYC | 2 cut(s) | 185, 2019 |
| BauI | CACGAG | 1 cut(s) | 2018 |
| BbsI | GAAGAC | 1 cut(s) | 273 |
| Bbv12I | GWGCWC | 4 cut(s) | 185, 237, 1054, 2019 |
| BbvI | GCAGC | 5 cut(s) | 304, 814, 1225, 2008, 2085 |
| BciT130I | CCWGG | 4 cut(s) | 272, 1425, 1473, 2012 |
| BclI | TGATCA | 1 cut(s) | 1710 |
| BcoDI | GTCTC | 6 cut(s) | 34, 558, 1414, 1415, 1496, 1943 |
| BfaI | CTAG | 3 cut(s) | 618, 1172, 1331 |
| BfmI | CTRYAG | 3 cut(s) | 220, 1485, 2099 |
| BfoI | RGCGCY | 1 cut(s) | 1513 |
| BfuAI | ACCTGC | 1 cut(s) | 1957 |
| BisI | GCNGC | 7 cut(s) | 318, 828, 1239, 1513, 1865, 1997, 2099 |
| BlsI | GCNGC | 7 cut(s) | 319, 829, 1240, 1514, 1866, 1998, 2100 |
| BmcAI | AGTACT | 1 cut(s) | 172 |
| Bme1390I | CCNGG | 4 cut(s) | 272, 1425, 1473, 2012 |
| Bme18I | GGWCC | 2 cut(s) | 686, 1430 |
| BmgT120I | GGNCC | 4 cut(s) | 307, 686, 1392, 1430 |
| BmiI | GGNNCC | 3 cut(s) | 101, 357, 1511 |
| BmrFI | CCNGG | 4 cut(s) | 272, 1425, 1473, 2012 |
| BmrI | ACTGGG | 1 cut(s) | 481 |
| BmsI | GCATC | 3 cut(s) | 722, 1123, 2085 |
| BmuI | ACTGGG | 1 cut(s) | 481 |
| BoxI | GACNNNNGTC | 1 cut(s) | 222 |
| BpiI | GAAGAC | 1 cut(s) | 273 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1135, 1167 |
| BpmI | CTGGAG | 1 cut(s) | 293 |
| BpuEI | CTTGAG | 2 cut(s) | 221, 1082 |
| Bsa29I | ATCGAT | 1 cut(s) | 2076 |
| BsaHI | GRCGYC | 1 cut(s) | 1510 |
| BsaI | GGTCTC | 1 cut(s) | 1414 |
| BsaJI | CCNNGG | 3 cut(s) | 33, 55, 682 |
| BsaWI | WCCGGW | 1 cut(s) | 1644 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 1472, 1594 |
| Bse118I | RCCGGY | 1 cut(s) | 1644 |
| Bse1I | ACTGG | 6 cut(s) | 68, 476, 1261, 1588, 1932, 2096 |
| Bse3DI | GCAATG | 1 cut(s) | 1563 |
| BseBI | CCWGG | 4 cut(s) | 272, 1425, 1473, 2012 |
| BseCI | ATCGAT | 1 cut(s) | 2076 |
| BseDI | CCNNGG | 3 cut(s) | 33, 55, 682 |
| BseGI | GGATG | 6 cut(s) | 154, 790, 1452, 1621, 2100, 2111 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 1472, 1594 |
| BseMI | GCAATG | 1 cut(s) | 1563 |
| BseMII | CTCAG | 4 cut(s) | 162, 837, 1583, 1938 |
| BseNI | ACTGG | 6 cut(s) | 68, 476, 1261, 1588, 1932, 2096 |
| BseSI | GKGCMC | 1 cut(s) | 1054 |
| BseXI | GCAGC | 5 cut(s) | 304, 814, 1225, 2008, 2085 |
| BsgI | GTGCAG | 1 cut(s) | 1800 |
| Bsh1236I | CGCG | 2 cut(s) | 988, 1851 |
| BshFI | GGCC | 8 cut(s) | 54, 308, 1170, 1393, 1679, 1838, 1945, 2088 |
| BshNI | GGYRCC | 1 cut(s) | 1509 |
| BshTI | ACCGGT | 1 cut(s) | 1644 |
| BshVI | ATCGAT | 1 cut(s) | 2076 |
| BsiHKAI | GWGCWC | 4 cut(s) | 185, 237, 1054, 2019 |
| BsiSI | CCGG | 1 cut(s) | 1645 |
| BslFI | GGGAC | 3 cut(s) | 664, 1529, 1938 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 1472, 1594 |
| BsmAI | GTCTC | 6 cut(s) | 34, 558, 1414, 1415, 1496, 1943 |
| BsmBI | CGTCTC | 3 cut(s) | 34, 1415, 1496 |
| BsmFI | GGGAC | 3 cut(s) | 664, 1529, 1938 |
| BsmI | GAATGC | 1 cut(s) | 1799 |
| BsnI | GGCC | 8 cut(s) | 54, 308, 1170, 1393, 1679, 1838, 1945, 2088 |
| Bso31I | GGTCTC | 1 cut(s) | 1414 |
| Bsp1286I | GDGCHC | 4 cut(s) | 185, 237, 1054, 2019 |
| Bsp1407I | TGTACA | 1 cut(s) | 1734 |
| Bsp143I | GATC | 5 cut(s) | 355, 752, 989, 1597, 1710 |
| Bsp19I | CCATGG | 1 cut(s) | 55 |
| Bsp68I | TCGCGA | 1 cut(s) | 1851 |
| BspACI | CCGC | 3 cut(s) | 1513, 1864, 1883 |
| BspANI | GGCC | 8 cut(s) | 54, 308, 1170, 1393, 1679, 1838, 1945, 2088 |
| BspCNI | CTCAG | 4 cut(s) | 161, 838, 1582, 1939 |
| BspDI | ATCGAT | 1 cut(s) | 2076 |
| BspFNI | CGCG | 2 cut(s) | 988, 1851 |
| BspHI | TCATGA | 3 cut(s) | 112, 892, 913 |
| BspLI | GGNNCC | 3 cut(s) | 101, 357, 1511 |
| BspMAI | CTGCAG | 1 cut(s) | 2103 |
| BspMI | ACCTGC | 1 cut(s) | 1957 |
| BspPI | GGATC | 3 cut(s) | 350, 363, 1605 |
| BspT107I | GGYRCC | 1 cut(s) | 1509 |
| BspTNI | GGTCTC | 1 cut(s) | 1414 |
| BsrDI | GCAATG | 1 cut(s) | 1563 |
| BsrFI | RCCGGY | 1 cut(s) | 1644 |
| BsrGI | TGTACA | 1 cut(s) | 1734 |
| BsrI | ACTGG | 6 cut(s) | 68, 476, 1261, 1588, 1932, 2096 |
| BssAI | RCCGGY | 1 cut(s) | 1644 |
| BssECI | CCNNGG | 3 cut(s) | 33, 55, 682 |
| BssMI | GATC | 5 cut(s) | 355, 752, 989, 1597, 1710 |
| BssNAI | GTATAC | 1 cut(s) | 1226 |
| BssNI | GRCGYC | 1 cut(s) | 1510 |
| BssSI | CACGAG | 1 cut(s) | 2018 |
| BssT1I | CCWWGG | 3 cut(s) | 33, 55, 682 |
| Bst1107I | GTATAC | 1 cut(s) | 1226 |
| Bst2BI | CACGAG | 1 cut(s) | 2018 |
| Bst2UI | CCWGG | 4 cut(s) | 272, 1425, 1473, 2012 |
| Bst4CI | ACNGT | 7 cut(s) | 221, 302, 699, 1672, 1699, 1788, 1821 |
| Bst6I | CTCTTC | 2 cut(s) | 1123, 1494 |
| BstACI | GRCGYC | 1 cut(s) | 1510 |
| BstAUI | TGTACA | 1 cut(s) | 1734 |
| BstC8I | GCNNGC | 6 cut(s) | 1066, 1180, 1189, 1681, 1987, 2148 |
| BstDEI | CTNAG | 7 cut(s) | 148, 717, 846, 900, 1530, 1569, 1947 |
| BstDSI | CCRYGG | 1 cut(s) | 55 |
| BstF5I | GGATG | 6 cut(s) | 154, 790, 1452, 1621, 2100, 2111 |
| BstFNI | CGCG | 2 cut(s) | 988, 1851 |
| BstH2I | RGCGCY | 1 cut(s) | 1513 |
| BstHHI | GCGC | 2 cut(s) | 988, 1512 |
| BstKTI | GATC | 5 cut(s) | 358, 755, 992, 1600, 1713 |
| BstMAI | GTCTC | 6 cut(s) | 34, 558, 1414, 1415, 1496, 1943 |
| BstMBI | GATC | 5 cut(s) | 355, 752, 989, 1597, 1710 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 314, 833, 961, 1184, 1188, 1340 |
| BstNI | CCWGG | 4 cut(s) | 272, 1425, 1473, 2012 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 222 |
| BstSCI | CCNGG | 4 cut(s) | 270, 1423, 1471, 2010 |
| BstSFI | CTRYAG | 3 cut(s) | 220, 1485, 2099 |
| BstSLI | GKGCMC | 1 cut(s) | 1054 |
| BstUI | CGCG | 2 cut(s) | 988, 1851 |
| BstV1I | GCAGC | 5 cut(s) | 304, 814, 1225, 2008, 2085 |
| BstV2I | GAAGAC | 1 cut(s) | 273 |
| BstX2I | RGATCY | 2 cut(s) | 355, 1597 |
| BstYI | RGATCY | 2 cut(s) | 355, 1597 |
| BstZ17I | GTATAC | 1 cut(s) | 1226 |
| Bsu15I | ATCGAT | 1 cut(s) | 2076 |
| BsuRI | GGCC | 8 cut(s) | 54, 308, 1170, 1393, 1679, 1838, 1945, 2088 |
| BsuTUI | ATCGAT | 1 cut(s) | 2076 |
| BtgI | CCRYGG | 1 cut(s) | 55 |
| BtsCI | GGATG | 6 cut(s) | 154, 790, 1452, 1621, 2100, 2111 |
| BtsI | GCAGTG | 2 cut(s) | 1276, 1967 |
| BtsIMutI | CAGTG | 5 cut(s) | 695, 1276, 1677, 1967, 2089 |
| BtuMI | TCGCGA | 1 cut(s) | 1851 |
| BveI | ACCTGC | 1 cut(s) | 1957 |
| Cac8I | GCNNGC | 6 cut(s) | 1066, 1180, 1189, 1681, 1987, 2148 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1241 |
| CciI | TCATGA | 3 cut(s) | 112, 892, 913 |
| CfoI | GCGC | 2 cut(s) | 988, 1512 |
| Cfr10I | RCCGGY | 1 cut(s) | 1644 |
| Cfr13I | GGNCC | 4 cut(s) | 307, 686, 1392, 1430 |
| ClaI | ATCGAT | 1 cut(s) | 2076 |
| CsiI | ACCWGGT | 2 cut(s) | 1423, 1471 |
| Csp6I | GTAC | 5 cut(s) | 171, 241, 667, 1555, 1735 |
| CspAI | ACCGGT | 1 cut(s) | 1644 |
| CviAII | CATG | 9 cut(s) | 56, 113, 500, 530, 676, 893, 914, 1742, 2125 |
| CviQI | GTAC | 5 cut(s) | 171, 241, 667, 1555, 1735 |
| DdeI | CTNAG | 7 cut(s) | 148, 717, 846, 900, 1530, 1569, 1947 |
| DinI | GGCGCC | 1 cut(s) | 1511 |
| DpnI | GATC | 5 cut(s) | 357, 754, 991, 1599, 1712 |
| DpnII | GATC | 5 cut(s) | 355, 752, 989, 1597, 1710 |
| EaeI | YGGCCR | 3 cut(s) | 52, 1677, 2086 |
| Eam1104I | CTCTTC | 2 cut(s) | 1123, 1494 |
| EarI | CTCTTC | 2 cut(s) | 1123, 1494 |
| Ecl136II | GAGCTC | 2 cut(s) | 183, 2017 |
| Eco130I | CCWWGG | 3 cut(s) | 33, 55, 682 |
| Eco147I | AGGCCT | 1 cut(s) | 1945 |
| Eco24I | GRGCYC | 2 cut(s) | 185, 2019 |
| Eco31I | GGTCTC | 1 cut(s) | 1414 |
| Eco47I | GGWCC | 2 cut(s) | 686, 1430 |
| Eco53kI | GAGCTC | 2 cut(s) | 183, 2017 |
| Eco57I | CTGAAG | 3 cut(s) | 471, 501, 561 |
| EcoICRI | GAGCTC | 2 cut(s) | 183, 2017 |
| EcoO109I | RGGNCCY | 1 cut(s) | 686 |
| EcoRII | CCWGG | 4 cut(s) | 270, 1423, 1471, 2010 |
| EcoT14I | CCWWGG | 3 cut(s) | 33, 55, 682 |
| EcoT38I | GRGCYC | 2 cut(s) | 185, 2019 |
| EgeI | GGCGCC | 1 cut(s) | 1511 |
| EheI | GGCGCC | 1 cut(s) | 1511 |
| ErhI | CCWWGG | 3 cut(s) | 33, 55, 682 |
| Esp3I | CGTCTC | 3 cut(s) | 34, 1415, 1496 |
| FaeI | CATG | 9 cut(s) | 59, 116, 503, 533, 679, 896, 917, 1745, 2128 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 160, 192 |
| FaqI | GGGAC | 3 cut(s) | 664, 1529, 1938 |
| FatI | CATG | 9 cut(s) | 55, 112, 499, 529, 675, 892, 913, 1741, 2124 |
| FbaI | TGATCA | 1 cut(s) | 1710 |
| FblI | GTMKAC | 1 cut(s) | 1225 |
| Fnu4HI | GCNGC | 7 cut(s) | 318, 828, 1239, 1513, 1865, 1997, 2099 |
| FokI | GGATG | 6 cut(s) | 141, 797, 1459, 1628, 2107, 2118 |
| FriOI | GRGCYC | 2 cut(s) | 185, 2019 |
| Fsp4HI | GCNGC | 7 cut(s) | 318, 828, 1239, 1513, 1865, 1997, 2099 |
| FspBI | CTAG | 3 cut(s) | 618, 1172, 1331 |
| GlaI | GCGC | 2 cut(s) | 987, 1511 |
| GluI | GCNGC | 7 cut(s) | 318, 828, 1239, 1513, 1865, 1997, 2099 |
| GsuI | CTGGAG | 1 cut(s) | 293 |
| HaeII | RGCGCY | 1 cut(s) | 1513 |
| HaeIII | GGCC | 8 cut(s) | 54, 308, 1170, 1393, 1679, 1838, 1945, 2088 |
| HapII | CCGG | 1 cut(s) | 1645 |
| HhaI | GCGC | 2 cut(s) | 988, 1512 |
| Hin1I | GRCGYC | 1 cut(s) | 1510 |
| Hin1II | CATG | 9 cut(s) | 59, 116, 503, 533, 679, 896, 917, 1745, 2128 |
| Hin6I | GCGC | 2 cut(s) | 986, 1510 |
| HinP1I | GCGC | 2 cut(s) | 986, 1510 |
| HindIII | AAGCTT | 2 cut(s) | 397, 1254 |
| HpaII | CCGG | 1 cut(s) | 1645 |
| HphI | GGTGA | 6 cut(s) | 357, 800, 1142, 1203, 1761, 2059 |
| Hpy166II | GTNNAC | 5 cut(s) | 73, 1052, 1091, 1226, 1430 |
| Hpy8I | GTNNAC | 5 cut(s) | 73, 1052, 1091, 1226, 1430 |
| HpyCH4III | ACNGT | 7 cut(s) | 221, 302, 699, 1672, 1699, 1788, 1821 |
| HpyCH4IV | ACGT | 1 cut(s) | 1408 |
| HpyCH4V | TGCA | 9 cut(s) | 295, 797, 1052, 1064, 1085, 1136, 1642, 1817, 2101 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 314, 833, 961, 1184, 1188, 1340 |
| HpyF3I | CTNAG | 7 cut(s) | 148, 717, 846, 900, 1530, 1569, 1947 |
| HpySE526I | ACGT | 1 cut(s) | 1408 |
| Hsp92I | GRCGYC | 1 cut(s) | 1510 |
| Hsp92II | CATG | 9 cut(s) | 59, 116, 503, 533, 679, 896, 917, 1745, 2128 |
| HspAI | GCGC | 2 cut(s) | 986, 1510 |
| KasI | GGCGCC | 1 cut(s) | 1509 |
| Ksp22I | TGATCA | 1 cut(s) | 1710 |
| Kzo9I | GATC | 5 cut(s) | 355, 752, 989, 1597, 1710 |
| LmnI | GCTCC | 3 cut(s) | 630, 2004, 2014 |
| Lsp1109I | GCAGC | 5 cut(s) | 304, 814, 1225, 2008, 2085 |
| LweI | GCATC | 3 cut(s) | 722, 1123, 2085 |
| MabI | ACCWGGT | 2 cut(s) | 1423, 1471 |
| MaeI | CTAG | 3 cut(s) | 618, 1172, 1331 |
| MaeII | ACGT | 1 cut(s) | 1408 |
| MaeIII | GTNAC | 4 cut(s) | 215, 434, 1270, 1296 |
| MalI | GATC | 5 cut(s) | 357, 754, 991, 1599, 1712 |
| MboI | GATC | 5 cut(s) | 355, 752, 989, 1597, 1710 |
| MfeI | CAATTG | 1 cut(s) | 1455 |
| MflI | RGATCY | 2 cut(s) | 355, 1597 |
| MhlI | GDGCHC | 4 cut(s) | 185, 237, 1054, 2019 |
| MlsI | TGGCCA | 3 cut(s) | 54, 1679, 2088 |
| MluNI | TGGCCA | 3 cut(s) | 54, 1679, 2088 |
| Mly113I | GGCGCC | 1 cut(s) | 1510 |
| MlyI | GAGTC | 3 cut(s) | 560, 836, 905 |
| MmeI | TCCRAC | 3 cut(s) | 164, 1335, 1429 |
| Mox20I | TGGCCA | 3 cut(s) | 54, 1679, 2088 |
| MscI | TGGCCA | 3 cut(s) | 54, 1679, 2088 |
| MseI | TTAA | 7 cut(s) | 345, 1250, 1602, 1632, 1649, 1722, 2152 |
| MslI | CAYNNNNRTG | 1 cut(s) | 159 |
| Msp20I | TGGCCA | 3 cut(s) | 54, 1679, 2088 |
| MspA1I | CMGCKG | 3 cut(s) | 827, 1238, 1864 |
| MspI | CCGG | 1 cut(s) | 1645 |
| MspR9I | CCNGG | 4 cut(s) | 272, 1425, 1473, 2012 |
| MunI | CAATTG | 1 cut(s) | 1455 |
| Mva1269I | GAATGC | 1 cut(s) | 1799 |
| MvaI | CCWGG | 4 cut(s) | 272, 1425, 1473, 2012 |
| MvnI | CGCG | 2 cut(s) | 988, 1851 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 314, 833, 961, 1184, 1188, 1340 |
| NarI | GGCGCC | 1 cut(s) | 1510 |
| NcoI | CCATGG | 1 cut(s) | 55 |
| NdeII | GATC | 5 cut(s) | 355, 752, 989, 1597, 1710 |
| NlaIII | CATG | 9 cut(s) | 59, 116, 503, 533, 679, 896, 917, 1745, 2128 |
| NlaIV | GGNNCC | 3 cut(s) | 101, 357, 1511 |
| NmuCI | GTSAC | 2 cut(s) | 215, 434 |
| NruI | TCGCGA | 1 cut(s) | 1851 |
| PagI | TCATGA | 3 cut(s) | 112, 892, 913 |
| PceI | AGGCCT | 1 cut(s) | 1945 |
| PctI | GAATGC | 1 cut(s) | 1799 |
| PfeI | GAWTC | 8 cut(s) | 164, 419, 482, 512, 581, 889, 1536, 2073 |
| PinAI | ACCGGT | 1 cut(s) | 1644 |
| PkrI | GCNGC | 7 cut(s) | 319, 829, 1240, 1514, 1866, 1998, 2100 |
| PleI | GAGTC | 3 cut(s) | 559, 836, 904 |
| PluTI | GGCGCC | 1 cut(s) | 1513 |
| PpsI | GAGTC | 3 cut(s) | 559, 836, 904 |
| PpuMI | RGGWCCY | 1 cut(s) | 686 |
| PshAI | GACNNNNGTC | 1 cut(s) | 222 |
| PshBI | ATTAAT | 1 cut(s) | 1722 |
| Psp124BI | GAGCTC | 2 cut(s) | 185, 2019 |
| Psp5II | RGGWCCY | 1 cut(s) | 686 |
| Psp6I | CCWGG | 4 cut(s) | 270, 1423, 1471, 2010 |
| PspGI | CCWGG | 4 cut(s) | 270, 1423, 1471, 2010 |
| PspN4I | GGNNCC | 3 cut(s) | 101, 357, 1511 |
| PspPI | GGNCC | 4 cut(s) | 307, 686, 1392, 1430 |
| PspPPI | RGGWCCY | 1 cut(s) | 686 |
| PstI | CTGCAG | 1 cut(s) | 2103 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1241 |
| PsuI | RGATCY | 2 cut(s) | 355, 1597 |
| PvuII | CAGCTG | 2 cut(s) | 827, 1238 |
| RruI | TCGCGA | 1 cut(s) | 1851 |
| RsaI | GTAC | 5 cut(s) | 172, 242, 668, 1556, 1736 |
| RsaNI | GTAC | 5 cut(s) | 171, 241, 667, 1555, 1735 |
| RseI | CAYNNNNRTG | 1 cut(s) | 159 |
| SacI | GAGCTC | 2 cut(s) | 185, 2019 |
| SaqAI | TTAA | 7 cut(s) | 345, 1250, 1602, 1632, 1649, 1722, 2152 |
| SatI | GCNGC | 7 cut(s) | 318, 828, 1239, 1513, 1865, 1997, 2099 |
| Sau3AI | GATC | 5 cut(s) | 355, 752, 989, 1597, 1710 |
| Sau96I | GGNCC | 4 cut(s) | 307, 686, 1392, 1430 |
| ScaI | AGTACT | 1 cut(s) | 172 |
| SchI | GAGTC | 3 cut(s) | 560, 836, 905 |
| ScrFI | CCNGG | 4 cut(s) | 272, 1425, 1473, 2012 |
| SduI | GDGCHC | 4 cut(s) | 185, 237, 1054, 2019 |
| SexAI | ACCWGGT | 2 cut(s) | 1423, 1471 |
| SfaNI | GCATC | 3 cut(s) | 722, 1123, 2085 |
| SfcI | CTRYAG | 3 cut(s) | 220, 1485, 2099 |
| SfoI | GGCGCC | 1 cut(s) | 1511 |
| SinI | GGWCC | 2 cut(s) | 686, 1430 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 159 |
| SmlI | CTYRAG | 2 cut(s) | 236, 1097 |
| SmoI | CTYRAG | 2 cut(s) | 236, 1097 |
| SseBI | AGGCCT | 1 cut(s) | 1945 |
| SsiI | CCGC | 3 cut(s) | 1513, 1864, 1883 |
| SspDI | GGCGCC | 1 cut(s) | 1509 |
| SspI | AATATT | 1 cut(s) | 1441 |
| SspMI | CTAG | 3 cut(s) | 618, 1172, 1331 |
| SstI | GAGCTC | 2 cut(s) | 185, 2019 |
| StuI | AGGCCT | 1 cut(s) | 1945 |
| StyD4I | CCNGG | 4 cut(s) | 270, 1423, 1471, 2010 |
| StyI | CCWWGG | 3 cut(s) | 33, 55, 682 |
| TaaI | ACNGT | 7 cut(s) | 221, 302, 699, 1672, 1699, 1788, 1821 |
| TaiI | ACGT | 1 cut(s) | 1411 |
| TaqI | TCGA | 3 cut(s) | 755, 1480, 2076 |
| TatI | WGTACW | 4 cut(s) | 170, 240, 1554, 1734 |
| TauI | GCSGC | 2 cut(s) | 1515, 1867 |
| TfiI | GAWTC | 8 cut(s) | 164, 419, 482, 512, 581, 889, 1536, 2073 |
| Tru1I | TTAA | 7 cut(s) | 345, 1250, 1602, 1632, 1649, 1722, 2152 |
| Tru9I | TTAA | 7 cut(s) | 345, 1250, 1602, 1632, 1649, 1722, 2152 |
| TscAI | CASTG | 5 cut(s) | 702, 1283, 1677, 1967, 2096 |
| TseFI | GTSAC | 2 cut(s) | 215, 434 |
| TseI | GCWGC | 5 cut(s) | 317, 827, 1238, 1996, 2098 |
| Tsp45I | GTSAC | 2 cut(s) | 215, 434 |
| TspGWI | ACGGA | 1 cut(s) | 16 |
| TspRI | CASTG | 5 cut(s) | 702, 1283, 1677, 1967, 2096 |
| VneI | GTGCAC | 1 cut(s) | 1050 |
| VpaK11BI | GGWCC | 2 cut(s) | 686, 1430 |
| VspI | ATTAAT | 1 cut(s) | 1722 |
| XapI | RAATTY | 4 cut(s) | 41, 595, 640, 706 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 53 |
| XmiI | GTMKAC | 1 cut(s) | 1225 |
| XspI | CTAG | 3 cut(s) | 618, 1172, 1331 |
| ZrmI | AGTACT | 1 cut(s) | 172 |