Belongs to the GRAS family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1681 |
| Acc36I | ACCTGC | 1 cut(s) | 1681 |
| Acc65I | GGTACC | 1 cut(s) | 570 |
| AccB1I | GGYRCC | 2 cut(s) | 570, 1410 |
| AccI | GTMKAC | 1 cut(s) | 924 |
| AccII | CGCG | 1 cut(s) | 1752 |
| AciI | CCGC | 1 cut(s) | 1414 |
| AclWI | GGATC | 5 cut(s) | 305, 318, 664, 883, 1092 |
| AcoI | YGGCCR | 1 cut(s) | 19 |
| AcsI | RAATTY | 5 cut(s) | 31, 523, 610, 1557, 1606 |
| AcuI | CTGAAG | 1 cut(s) | 129 |
| AcyI | GRCGYC | 2 cut(s) | 1411, 1596 |
| AfaI | GTAC | 4 cut(s) | 197, 572, 580, 605 |
| AfeI | AGCGCT | 1 cut(s) | 1719 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 1373, 1495 |
| AflIII | ACRYGT | 1 cut(s) | 887 |
| AgeI | ACCGGT | 1 cut(s) | 1545 |
| AjnI | CCWGG | 3 cut(s) | 225, 586, 1372 |
| AjuI | GAANNNNNNNTTGG | 3 cut(s) | 32, 1362, 1394 |
| Alw21I | GWGCWC | 3 cut(s) | 192, 955, 1920 |
| Alw26I | GTCTC | 6 cut(s) | 43, 471, 1316, 1397, 1587, 1844 |
| Alw44I | GTGCAC | 1 cut(s) | 951 |
| AlwI | GGATC | 5 cut(s) | 305, 318, 664, 883, 1092 |
| Aor51HI | AGCGCT | 1 cut(s) | 1719 |
| AoxI | GGCC | 6 cut(s) | 19, 262, 1069, 1292, 1737, 1844 |
| ApaLI | GTGCAC | 1 cut(s) | 951 |
| ApeKI | GCWGC | 7 cut(s) | 272, 401, 414, 731, 853, 1897, 1999 |
| ApoI | RAATTY | 5 cut(s) | 31, 523, 610, 1557, 1606 |
| AseI | ATTAAT | 1 cut(s) | 1623 |
| AsiGI | ACCGGT | 1 cut(s) | 1545 |
| Asp718I | GGTACC | 1 cut(s) | 570 |
| AspLEI | GCGC | 2 cut(s) | 1413, 1720 |
| AspS9I | GGNCC | 4 cut(s) | 262, 590, 1293, 1331 |
| AsuC2I | CCSGG | 1 cut(s) | 1913 |
| AsuHPI | GGTGA | 2 cut(s) | 164, 2000 |
| AsuII | TTCGAA | 1 cut(s) | 1776 |
| AvaII | GGWCC | 2 cut(s) | 590, 1331 |
| BaeGI | GKGCMC | 1 cut(s) | 955 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 562, 595 |
| BalI | TGGCCA | 1 cut(s) | 21 |
| BamHI | GGATCC | 1 cut(s) | 310 |
| BanI | GGYRCC | 2 cut(s) | 570, 1410 |
| BanII | GRGCYC | 2 cut(s) | 69, 1920 |
| BbsI | GAAGAC | 2 cut(s) | 228, 1940 |
| Bbv12I | GWGCWC | 3 cut(s) | 192, 955, 1920 |
| BbvCI | CCTCAGC | 1 cut(s) | 834 |
| BbvI | GCAGC | 7 cut(s) | 259, 388, 426, 718, 865, 1909, 1986 |
| BccI | CCATC | 5 cut(s) | 362, 1493, 1945, 1979, 2001 |
| BceAI | ACGGC | 1 cut(s) | 1583 |
| BciT130I | CCWGG | 3 cut(s) | 227, 588, 1374 |
| BclI | TGATCA | 1 cut(s) | 46 |
| BcnI | CCSGG | 1 cut(s) | 1913 |
| BcoDI | GTCTC | 6 cut(s) | 43, 471, 1316, 1397, 1587, 1844 |
| BfaI | CTAG | 5 cut(s) | 126, 215, 843, 1073, 1872 |
| BfmI | CTRYAG | 1 cut(s) | 2000 |
| BfoI | RGCGCY | 2 cut(s) | 1414, 1721 |
| BfuAI | ACCTGC | 1 cut(s) | 1681 |
| BisI | GCNGC | 8 cut(s) | 273, 402, 415, 732, 854, 1414, 1898, 2000 |
| BlpI | GCTNAGC | 1 cut(s) | 1417 |
| BlsI | GCNGC | 8 cut(s) | 274, 403, 416, 733, 855, 1415, 1899, 2001 |
| Bme1390I | CCNGG | 4 cut(s) | 227, 588, 1374, 1913 |
| Bme18I | GGWCC | 2 cut(s) | 590, 1331 |
| BmgT120I | GGNCC | 4 cut(s) | 262, 590, 1293, 1331 |
| BmiI | GGNNCC | 4 cut(s) | 68, 312, 572, 1412 |
| BmrFI | CCNGG | 4 cut(s) | 227, 588, 1374, 1913 |
| BmsI | GCATC | 4 cut(s) | 283, 626, 1142, 1986 |
| BpiI | GAAGAC | 2 cut(s) | 228, 1940 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 455, 487, 1036, 1068 |
| BpmI | CTGGAG | 3 cut(s) | 248, 570, 1514 |
| Bpu10I | CCTNAGC | 1 cut(s) | 834 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1417 |
| Bpu14I | TTCGAA | 1 cut(s) | 1776 |
| BpuEI | CTTGAG | 3 cut(s) | 176, 584, 983 |
| BpuMI | CCSGG | 1 cut(s) | 1913 |
| BsaBI | GATNNNNATC | 3 cut(s) | 750, 1102, 1761 |
| BsaHI | GRCGYC | 2 cut(s) | 1411, 1596 |
| BsaJI | CCNNGG | 1 cut(s) | 22 |
| BsaWI | WCCGGW | 1 cut(s) | 1545 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 1373, 1495 |
| Bse118I | RCCGGY | 2 cut(s) | 912, 1545 |
| Bse1I | ACTGG | 3 cut(s) | 574, 1497, 1833 |
| Bse3DI | GCAATG | 1 cut(s) | 1464 |
| Bse8I | GATNNNNATC | 3 cut(s) | 750, 1102, 1761 |
| BseBI | CCWGG | 3 cut(s) | 227, 588, 1374 |
| BseDI | CCNNGG | 1 cut(s) | 22 |
| BseGI | GGATG | 4 cut(s) | 617, 1485, 2001, 2012 |
| BseJI | GATNNNNATC | 3 cut(s) | 750, 1102, 1761 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 1373, 1495 |
| BseMI | GCAATG | 1 cut(s) | 1464 |
| BseMII | CTCAG | 4 cut(s) | 378, 825, 1329, 1839 |
| BseNI | ACTGG | 3 cut(s) | 574, 1497, 1833 |
| BseSI | GKGCMC | 1 cut(s) | 955 |
| BseXI | GCAGC | 7 cut(s) | 259, 388, 426, 718, 865, 1909, 1986 |
| BseYI | CCCAGC | 1 cut(s) | 1886 |
| Bsh1236I | CGCG | 1 cut(s) | 1752 |
| BshFI | GGCC | 6 cut(s) | 21, 264, 1071, 1294, 1739, 1846 |
| BshNI | GGYRCC | 2 cut(s) | 570, 1410 |
| BshTI | ACCGGT | 1 cut(s) | 1545 |
| BsiHKAI | GWGCWC | 3 cut(s) | 192, 955, 1920 |
| BsiSI | CCGG | 3 cut(s) | 913, 1546, 1912 |
| BslFI | GGGAC | 5 cut(s) | 1430, 1672, 1730, 1811, 1839 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 1373, 1495 |
| BsmAI | GTCTC | 6 cut(s) | 43, 471, 1316, 1397, 1587, 1844 |
| BsmBI | CGTCTC | 1 cut(s) | 1587 |
| BsmFI | GGGAC | 5 cut(s) | 1430, 1672, 1730, 1811, 1839 |
| BsmI | GAATGC | 1 cut(s) | 1015 |
| BsnI | GGCC | 6 cut(s) | 21, 264, 1071, 1294, 1739, 1846 |
| Bsp119I | TTCGAA | 1 cut(s) | 1776 |
| Bsp1286I | GDGCHC | 4 cut(s) | 69, 192, 955, 1920 |
| Bsp1407I | TGTACA | 2 cut(s) | 578, 603 |
| Bsp143I | GATC | 6 cut(s) | 46, 310, 656, 751, 875, 1097 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1417 |
| Bsp19I | CCATGG | 1 cut(s) | 22 |
| Bsp68I | TCGCGA | 1 cut(s) | 1752 |
| BspACI | CCGC | 1 cut(s) | 1414 |
| BspANI | GGCC | 6 cut(s) | 21, 264, 1071, 1294, 1739, 1846 |
| BspCNI | CTCAG | 4 cut(s) | 377, 826, 1328, 1840 |
| BspFNI | CGCG | 1 cut(s) | 1752 |
| BspHI | TCATGA | 2 cut(s) | 79, 814 |
| BspLI | GGNNCC | 4 cut(s) | 68, 312, 572, 1412 |
| BspMAI | CTGCAG | 1 cut(s) | 2004 |
| BspMI | ACCTGC | 1 cut(s) | 1681 |
| BspPI | GGATC | 5 cut(s) | 305, 318, 664, 883, 1092 |
| BspQI | GCTCTTC | 1 cut(s) | 1709 |
| BspT104I | TTCGAA | 1 cut(s) | 1776 |
| BspT107I | GGYRCC | 2 cut(s) | 570, 1410 |
| BsrDI | GCAATG | 1 cut(s) | 1464 |
| BsrFI | RCCGGY | 2 cut(s) | 912, 1545 |
| BsrGI | TGTACA | 2 cut(s) | 578, 603 |
| BsrI | ACTGG | 3 cut(s) | 574, 1497, 1833 |
| BssAI | RCCGGY | 2 cut(s) | 912, 1545 |
| BssECI | CCNNGG | 1 cut(s) | 22 |
| BssMI | GATC | 6 cut(s) | 46, 310, 656, 751, 875, 1097 |
| BssNI | GRCGYC | 2 cut(s) | 1411, 1596 |
| BssT1I | CCWWGG | 1 cut(s) | 22 |
| Bst2UI | CCWGG | 3 cut(s) | 227, 588, 1374 |
| Bst4CI | ACNGT | 9 cut(s) | 122, 160, 167, 603, 1538, 1573, 1689, 1698, 1744 |
| Bst6I | CTCTTC | 4 cut(s) | 46, 1122, 1395, 1709 |
| BstACI | GRCGYC | 2 cut(s) | 1411, 1596 |
| BstAUI | TGTACA | 2 cut(s) | 578, 603 |
| BstBI | TTCGAA | 1 cut(s) | 1776 |
| BstC8I | GCNNGC | 5 cut(s) | 1081, 1131, 1180, 1869, 2049 |
| BstDSI | CCRYGG | 1 cut(s) | 22 |
| BstF5I | GGATG | 4 cut(s) | 617, 1485, 2001, 2012 |
| BstFNI | CGCG | 1 cut(s) | 1752 |
| BstH2I | RGCGCY | 2 cut(s) | 1414, 1721 |
| BstHHI | GCGC | 2 cut(s) | 1413, 1720 |
| BstKTI | GATC | 6 cut(s) | 49, 313, 659, 754, 878, 1100 |
| BstMAI | GTCTC | 6 cut(s) | 43, 471, 1316, 1397, 1587, 1844 |
| BstMBI | GATC | 6 cut(s) | 46, 310, 656, 751, 875, 1097 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 390, 737, 862, 1085, 2036 |
| BstNI | CCWGG | 3 cut(s) | 227, 588, 1374 |
| BstNSI | RCATGY | 3 cut(s) | 247, 891, 1182 |
| BstSCI | CCNGG | 4 cut(s) | 225, 586, 1372, 1911 |
| BstSFI | CTRYAG | 1 cut(s) | 2000 |
| BstSLI | GKGCMC | 1 cut(s) | 955 |
| BstUI | CGCG | 1 cut(s) | 1752 |
| BstV1I | GCAGC | 7 cut(s) | 259, 388, 426, 718, 865, 1909, 1986 |
| BstV2I | GAAGAC | 2 cut(s) | 228, 1940 |
| BstX2I | RGATCY | 3 cut(s) | 310, 875, 1097 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1492 |
| BstYI | RGATCY | 3 cut(s) | 310, 875, 1097 |
| BsuRI | GGCC | 6 cut(s) | 21, 264, 1071, 1294, 1739, 1846 |
| BtgI | CCRYGG | 1 cut(s) | 22 |
| BtsCI | GGATG | 4 cut(s) | 617, 1485, 2001, 2012 |
| BtsI | GCAGTG | 1 cut(s) | 1868 |
| BtsIMutI | CAGTG | 4 cut(s) | 581, 599, 1740, 1868 |
| BtuMI | TCGCGA | 1 cut(s) | 1752 |
| BveI | ACCTGC | 1 cut(s) | 1681 |
| Cac8I | GCNNGC | 5 cut(s) | 1081, 1131, 1180, 1869, 2049 |
| CciI | TCATGA | 2 cut(s) | 79, 814 |
| CfoI | GCGC | 2 cut(s) | 1413, 1720 |
| Cfr10I | RCCGGY | 2 cut(s) | 912, 1545 |
| Cfr13I | GGNCC | 4 cut(s) | 262, 590, 1293, 1331 |
| CseI | GACGC | 1 cut(s) | 1604 |
| CsiI | ACCWGGT | 1 cut(s) | 1372 |
| Csp6I | GTAC | 4 cut(s) | 196, 571, 579, 604 |
| CspAI | ACCGGT | 1 cut(s) | 1545 |
| CviAII | CATG | 9 cut(s) | 23, 80, 244, 443, 815, 888, 1179, 1639, 1643 |
| CviQI | GTAC | 4 cut(s) | 196, 571, 579, 604 |
| DinI | GGCGCC | 1 cut(s) | 1412 |
| DpnI | GATC | 6 cut(s) | 48, 312, 658, 753, 877, 1099 |
| DpnII | GATC | 6 cut(s) | 46, 310, 656, 751, 875, 1097 |
| DraI | TTTAAA | 1 cut(s) | 828 |
| EaeI | YGGCCR | 1 cut(s) | 19 |
| Eam1104I | CTCTTC | 4 cut(s) | 46, 1122, 1395, 1709 |
| EarI | CTCTTC | 4 cut(s) | 46, 1122, 1395, 1709 |
| Ecl136II | GAGCTC | 1 cut(s) | 1918 |
| Eco130I | CCWWGG | 1 cut(s) | 22 |
| Eco147I | AGGCCT | 2 cut(s) | 1071, 1846 |
| Eco24I | GRGCYC | 2 cut(s) | 69, 1920 |
| Eco47I | GGWCC | 2 cut(s) | 590, 1331 |
| Eco47III | AGCGCT | 1 cut(s) | 1719 |
| Eco53kI | GAGCTC | 1 cut(s) | 1918 |
| Eco57I | CTGAAG | 1 cut(s) | 129 |
| EcoICRI | GAGCTC | 1 cut(s) | 1918 |
| EcoO109I | RGGNCCY | 1 cut(s) | 590 |
| EcoRII | CCWGG | 3 cut(s) | 225, 586, 1372 |
| EcoT14I | CCWWGG | 1 cut(s) | 22 |
| EcoT22I | ATGCAT | 1 cut(s) | 1644 |
| EcoT38I | GRGCYC | 2 cut(s) | 69, 1920 |
| EgeI | GGCGCC | 1 cut(s) | 1412 |
| EheI | GGCGCC | 1 cut(s) | 1412 |
| ErhI | CCWWGG | 1 cut(s) | 22 |
| Esp3I | CGTCTC | 1 cut(s) | 1587 |
| FaeI | CATG | 9 cut(s) | 26, 83, 247, 446, 818, 891, 1182, 1642, 1646 |
| FaqI | GGGAC | 5 cut(s) | 1430, 1672, 1730, 1811, 1839 |
| FatI | CATG | 9 cut(s) | 22, 79, 243, 442, 814, 887, 1178, 1638, 1642 |
| FbaI | TGATCA | 1 cut(s) | 46 |
| FblI | GTMKAC | 1 cut(s) | 924 |
| Fnu4HI | GCNGC | 8 cut(s) | 273, 402, 415, 732, 854, 1414, 1898, 2000 |
| FokI | GGATG | 4 cut(s) | 604, 1472, 2008, 2019 |
| FriOI | GRGCYC | 2 cut(s) | 69, 1920 |
| Fsp4HI | GCNGC | 8 cut(s) | 273, 402, 415, 732, 854, 1414, 1898, 2000 |
| FspBI | CTAG | 5 cut(s) | 126, 215, 843, 1073, 1872 |
| GlaI | GCGC | 2 cut(s) | 1412, 1719 |
| GluI | GCNGC | 8 cut(s) | 273, 402, 415, 732, 854, 1414, 1898, 2000 |
| GsaI | CCCAGC | 1 cut(s) | 1890 |
| GsuI | CTGGAG | 3 cut(s) | 248, 570, 1514 |
| HaeII | RGCGCY | 2 cut(s) | 1414, 1721 |
| HaeIII | GGCC | 6 cut(s) | 21, 264, 1071, 1294, 1739, 1846 |
| HapII | CCGG | 3 cut(s) | 913, 1546, 1912 |
| HgaI | GACGC | 1 cut(s) | 1604 |
| HhaI | GCGC | 2 cut(s) | 1413, 1720 |
| Hin1I | GRCGYC | 2 cut(s) | 1411, 1596 |
| Hin1II | CATG | 9 cut(s) | 26, 83, 247, 446, 818, 891, 1182, 1642, 1646 |
| Hin6I | GCGC | 2 cut(s) | 1411, 1718 |
| HinP1I | GCGC | 2 cut(s) | 1411, 1718 |
| HincII | GTYRAC | 2 cut(s) | 163, 1534 |
| HindII | GTYRAC | 2 cut(s) | 163, 1534 |
| HindIII | AAGCTT | 1 cut(s) | 352 |
| HinfI | GANTC | 9 cut(s) | 131, 464, 494, 673, 746, 790, 797, 803, 821 |
| HpaI | GTTAAC | 1 cut(s) | 1534 |
| HpaII | CCGG | 3 cut(s) | 913, 1546, 1912 |
| HphI | GGTGA | 2 cut(s) | 164, 2000 |
| Hpy166II | GTNNAC | 9 cut(s) | 163, 579, 581, 925, 953, 992, 1286, 1331, 1534 |
| Hpy188III | TCNNGA | 8 cut(s) | 80, 215, 794, 815, 1189, 1562, 1628, 1751 |
| Hpy8I | GTNNAC | 9 cut(s) | 163, 579, 581, 925, 953, 992, 1286, 1331, 1534 |
| HpyAV | CCTTC | 8 cut(s) | 301, 536, 683, 701, 1061, 1195, 1234, 2010 |
| HpyCH4III | ACNGT | 9 cut(s) | 122, 160, 167, 603, 1538, 1573, 1689, 1698, 1744 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 390, 737, 862, 1085, 2036 |
| Hsp92I | GRCGYC | 2 cut(s) | 1411, 1596 |
| Hsp92II | CATG | 9 cut(s) | 26, 83, 247, 446, 818, 891, 1182, 1642, 1646 |
| HspAI | GCGC | 2 cut(s) | 1411, 1718 |
| KasI | GGCGCC | 1 cut(s) | 1410 |
| KpnI | GGTACC | 1 cut(s) | 574 |
| Ksp22I | TGATCA | 1 cut(s) | 46 |
| KspAI | GTTAAC | 1 cut(s) | 1534 |
| Kzo9I | GATC | 6 cut(s) | 46, 310, 656, 751, 875, 1097 |
| LguI | GCTCTTC | 1 cut(s) | 1709 |
| LmnI | GCTCC | 3 cut(s) | 72, 1905, 1915 |
| Lsp1109I | GCAGC | 7 cut(s) | 259, 388, 426, 718, 865, 1909, 1986 |
| LweI | GCATC | 4 cut(s) | 283, 626, 1142, 1986 |
| MabI | ACCWGGT | 1 cut(s) | 1372 |
| MaeI | CTAG | 5 cut(s) | 126, 215, 843, 1073, 1872 |
| MaeIII | GTNAC | 3 cut(s) | 170, 1197, 1681 |
| MalI | GATC | 6 cut(s) | 48, 312, 658, 753, 877, 1099 |
| MboI | GATC | 6 cut(s) | 46, 310, 656, 751, 875, 1097 |
| MfeI | CAATTG | 1 cut(s) | 1356 |
| MflI | RGATCY | 3 cut(s) | 310, 875, 1097 |
| MhlI | GDGCHC | 4 cut(s) | 69, 192, 955, 1920 |
| MlsI | TGGCCA | 1 cut(s) | 21 |
| MluNI | TGGCCA | 1 cut(s) | 21 |
| Mly113I | GGCGCC | 1 cut(s) | 1411 |
| MlyI | GAGTC | 2 cut(s) | 473, 812 |
| MmeI | TCCRAC | 2 cut(s) | 228, 1513 |
| Mox20I | TGGCCA | 1 cut(s) | 21 |
| Mph1103I | ATGCAT | 1 cut(s) | 1644 |
| MscI | TGGCCA | 1 cut(s) | 21 |
| MseI | TTAA | 9 cut(s) | 12, 300, 827, 1151, 1533, 1604, 1614, 1623, 1923 |
| Msp20I | TGGCCA | 1 cut(s) | 21 |
| MspA1I | CMGCKG | 1 cut(s) | 731 |
| MspI | CCGG | 3 cut(s) | 913, 1546, 1912 |
| MspR9I | CCNGG | 4 cut(s) | 227, 588, 1374, 1913 |
| MunI | CAATTG | 1 cut(s) | 1356 |
| Mva1269I | GAATGC | 1 cut(s) | 1015 |
| MvaI | CCWGG | 3 cut(s) | 227, 588, 1374 |
| MvnI | CGCG | 1 cut(s) | 1752 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 390, 737, 862, 1085, 2036 |
| NarI | GGCGCC | 1 cut(s) | 1411 |
| NciI | CCSGG | 1 cut(s) | 1913 |
| NcoI | CCATGG | 1 cut(s) | 22 |
| NdeII | GATC | 6 cut(s) | 46, 310, 656, 751, 875, 1097 |
| NlaIII | CATG | 9 cut(s) | 26, 83, 247, 446, 818, 891, 1182, 1642, 1646 |
| NlaIV | GGNNCC | 4 cut(s) | 68, 312, 572, 1412 |
| NmuCI | GTSAC | 2 cut(s) | 170, 1681 |
| NruI | TCGCGA | 1 cut(s) | 1752 |
| NsiI | ATGCAT | 1 cut(s) | 1644 |
| NspI | RCATGY | 3 cut(s) | 247, 891, 1182 |
| NspV | TTCGAA | 1 cut(s) | 1776 |
| PaeI | GCATGC | 1 cut(s) | 1182 |
| PagI | TCATGA | 2 cut(s) | 79, 814 |
| PaqCI | CACCTGC | 1 cut(s) | 1681 |
| PceI | AGGCCT | 2 cut(s) | 1071, 1846 |
| PciI | ACATGT | 1 cut(s) | 887 |
| PciSI | GCTCTTC | 1 cut(s) | 1709 |
| PctI | GAATGC | 1 cut(s) | 1015 |
| PfeI | GAWTC | 7 cut(s) | 131, 494, 673, 746, 790, 797, 821 |
| PflFI | GACNNNGTC | 2 cut(s) | 1598, 1939 |
| PinAI | ACCGGT | 1 cut(s) | 1545 |
| PkrI | GCNGC | 8 cut(s) | 274, 403, 416, 733, 855, 1415, 1899, 2001 |
| PleI | GAGTC | 2 cut(s) | 472, 811 |
| PluTI | GGCGCC | 1 cut(s) | 1414 |
| PpsI | GAGTC | 2 cut(s) | 472, 811 |
| PpuMI | RGGWCCY | 1 cut(s) | 590 |
| PscI | ACATGT | 1 cut(s) | 887 |
| PshBI | ATTAAT | 1 cut(s) | 1623 |
| Psp124BI | GAGCTC | 1 cut(s) | 1920 |
| Psp5II | RGGWCCY | 1 cut(s) | 590 |
| Psp6I | CCWGG | 3 cut(s) | 225, 586, 1372 |
| PspFI | CCCAGC | 1 cut(s) | 1886 |
| PspGI | CCWGG | 3 cut(s) | 225, 586, 1372 |
| PspN4I | GGNNCC | 4 cut(s) | 68, 312, 572, 1412 |
| PspPI | GGNCC | 4 cut(s) | 262, 590, 1293, 1331 |
| PspPPI | RGGWCCY | 1 cut(s) | 590 |
| PstI | CTGCAG | 1 cut(s) | 2004 |
| PsuI | RGATCY | 3 cut(s) | 310, 875, 1097 |
| PsyI | GACNNNGTC | 2 cut(s) | 1598, 1939 |
| PvuII | CAGCTG | 1 cut(s) | 731 |
| RruI | TCGCGA | 1 cut(s) | 1752 |
| RsaI | GTAC | 4 cut(s) | 197, 572, 580, 605 |
| RsaNI | GTAC | 4 cut(s) | 196, 571, 579, 604 |
| SacI | GAGCTC | 1 cut(s) | 1920 |
| SapI | GCTCTTC | 1 cut(s) | 1709 |
| SaqAI | TTAA | 9 cut(s) | 12, 300, 827, 1151, 1533, 1604, 1614, 1623, 1923 |
| SatI | GCNGC | 8 cut(s) | 273, 402, 415, 732, 854, 1414, 1898, 2000 |
| Sau3AI | GATC | 6 cut(s) | 46, 310, 656, 751, 875, 1097 |
| Sau96I | GGNCC | 4 cut(s) | 262, 590, 1293, 1331 |
| SchI | GAGTC | 2 cut(s) | 473, 812 |
| ScrFI | CCNGG | 4 cut(s) | 227, 588, 1374, 1913 |
| SduI | GDGCHC | 4 cut(s) | 69, 192, 955, 1920 |
| SexAI | ACCWGGT | 1 cut(s) | 1372 |
| SfaNI | GCATC | 4 cut(s) | 283, 626, 1142, 1986 |
| SfcI | CTRYAG | 1 cut(s) | 2000 |
| SfoI | GGCGCC | 1 cut(s) | 1412 |
| SfuI | TTCGAA | 1 cut(s) | 1776 |
| SinI | GGWCC | 2 cut(s) | 590, 1331 |
| SmlI | CTYRAG | 3 cut(s) | 191, 563, 998 |
| SmoI | CTYRAG | 3 cut(s) | 191, 563, 998 |
| SphI | GCATGC | 1 cut(s) | 1182 |
| SseBI | AGGCCT | 2 cut(s) | 1071, 1846 |
| SsiI | CCGC | 1 cut(s) | 1414 |
| SspDI | GGCGCC | 1 cut(s) | 1410 |
| SspI | AATATT | 2 cut(s) | 1427, 1788 |
| SspMI | CTAG | 5 cut(s) | 126, 215, 843, 1073, 1872 |
| SstI | GAGCTC | 1 cut(s) | 1920 |
| StuI | AGGCCT | 2 cut(s) | 1071, 1846 |
| StyD4I | CCNGG | 4 cut(s) | 225, 586, 1372, 1911 |
| StyI | CCWWGG | 1 cut(s) | 22 |
| TaaI | ACNGT | 9 cut(s) | 122, 160, 167, 603, 1538, 1573, 1689, 1698, 1744 |
| TaqI | TCGA | 4 cut(s) | 1381, 1725, 1776, 1943 |
| TatI | WGTACW | 3 cut(s) | 195, 578, 603 |
| TauI | GCSGC | 1 cut(s) | 1416 |
| TfiI | GAWTC | 7 cut(s) | 131, 494, 673, 746, 790, 797, 821 |
| Tru1I | TTAA | 9 cut(s) | 12, 300, 827, 1151, 1533, 1604, 1614, 1623, 1923 |
| Tru9I | TTAA | 9 cut(s) | 12, 300, 827, 1151, 1533, 1604, 1614, 1623, 1923 |
| TscAI | CASTG | 4 cut(s) | 581, 606, 1747, 1868 |
| TseFI | GTSAC | 2 cut(s) | 170, 1681 |
| TseI | GCWGC | 7 cut(s) | 272, 401, 414, 731, 853, 1897, 1999 |
| Tsp45I | GTSAC | 2 cut(s) | 170, 1681 |
| TspGWI | ACGGA | 1 cut(s) | 610 |
| TspRI | CASTG | 4 cut(s) | 581, 606, 1747, 1868 |
| Tth111I | GACNNNGTC | 2 cut(s) | 1598, 1939 |
| VneI | GTGCAC | 1 cut(s) | 951 |
| VpaK11BI | GGWCC | 2 cut(s) | 590, 1331 |
| VspI | ATTAAT | 1 cut(s) | 1623 |
| XapI | RAATTY | 5 cut(s) | 31, 523, 610, 1557, 1606 |
| XbaI | TCTAGA | 1 cut(s) | 214 |
| XceI | RCATGY | 3 cut(s) | 247, 891, 1182 |
| XmiI | GTMKAC | 1 cut(s) | 924 |
| XspI | CTAG | 5 cut(s) | 126, 215, 843, 1073, 1872 |
| Zsp2I | ATGCAT | 1 cut(s) | 1644 |