Belongs to the GRAS family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1840 |
| Acc65I | GGTACC | 1 cut(s) | 543 |
| AccB1I | GGYRCC | 1 cut(s) | 543 |
| AccI | GTMKAC | 1 cut(s) | 1890 |
| AccII | CGCG | 1 cut(s) | 871 |
| AciI | CCGC | 3 cut(s) | 737, 1046, 1747 |
| AclWI | GGATC | 3 cut(s) | 646, 865, 1488 |
| AcoI | YGGCCR | 1 cut(s) | 1969 |
| AcsI | RAATTY | 1 cut(s) | 1920 |
| AcyI | GRCGYC | 1 cut(s) | 1393 |
| AfaI | GTAC | 5 cut(s) | 131, 152, 545, 586, 1439 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 689, 1355, 1477 |
| AflIII | ACRYGT | 1 cut(s) | 406 |
| AgeI | ACCGGT | 1 cut(s) | 546 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1392 |
| AhlI | ACTAGT | 1 cut(s) | 901 |
| AjnI | CCWGG | 1 cut(s) | 1354 |
| AleI | CACNNNNGTG | 1 cut(s) | 69 |
| Alw21I | GWGCWC | 4 cut(s) | 95, 300, 917, 937 |
| Alw26I | GTCTC | 5 cut(s) | 1297, 1379, 1544, 1569, 1826 |
| Alw44I | GTGCAC | 2 cut(s) | 296, 933 |
| AlwI | GGATC | 3 cut(s) | 646, 865, 1488 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 443, 1124 |
| AoxI | GGCC | 7 cut(s) | 213, 575, 1051, 1274, 1719, 1826, 1969 |
| ApaLI | GTGCAC | 2 cut(s) | 296, 933 |
| ApeKI | GCWGC | 5 cut(s) | 224, 706, 838, 994, 1121 |
| ApoI | RAATTY | 1 cut(s) | 1920 |
| AseI | ATTAAT | 1 cut(s) | 1605 |
| AsiGI | ACCGGT | 1 cut(s) | 546 |
| Asp718I | GGTACC | 1 cut(s) | 543 |
| AspLEI | GCGC | 2 cut(s) | 843, 871 |
| AspS9I | GGNCC | 4 cut(s) | 61, 214, 1275, 1313 |
| AsuC2I | CCSGG | 2 cut(s) | 561, 1308 |
| AsuHPI | GGTGA | 5 cut(s) | 264, 1025, 1516, 1573, 1644 |
| AsuII | TTCGAA | 1 cut(s) | 1758 |
| AvaII | GGWCC | 2 cut(s) | 61, 1313 |
| BaeGI | GKGCMC | 2 cut(s) | 300, 937 |
| BalI | TGGCCA | 1 cut(s) | 1971 |
| BanI | GGYRCC | 1 cut(s) | 543 |
| BanII | GRGCYC | 1 cut(s) | 95 |
| BauI | CACGAG | 1 cut(s) | 1731 |
| BbsI | GAAGAC | 2 cut(s) | 180, 377 |
| Bbv12I | GWGCWC | 4 cut(s) | 95, 300, 917, 937 |
| BbvI | GCAGC | 5 cut(s) | 211, 718, 850, 1006, 1108 |
| BccI | CCATC | 9 cut(s) | 17, 98, 273, 611, 1043, 1093, 1552, 1961, 1983 |
| BceAI | ACGGC | 1 cut(s) | 1760 |
| BciT130I | CCWGG | 1 cut(s) | 1356 |
| BclI | TGATCA | 2 cut(s) | 568, 1593 |
| BcnI | CCSGG | 2 cut(s) | 561, 1308 |
| BcoDI | GTCTC | 5 cut(s) | 1297, 1379, 1544, 1569, 1826 |
| BcuI | ACTAGT | 1 cut(s) | 901 |
| BfaI | CTAG | 6 cut(s) | 167, 902, 1055, 1214, 1230, 2035 |
| BfmI | CTRYAG | 2 cut(s) | 1368, 1982 |
| BfuAI | ACCTGC | 1 cut(s) | 1840 |
| BisI | GCNGC | 7 cut(s) | 225, 707, 737, 839, 995, 1122, 1748 |
| BlsI | GCNGC | 7 cut(s) | 226, 708, 738, 840, 996, 1123, 1749 |
| BmcAI | AGTACT | 1 cut(s) | 586 |
| Bme1390I | CCNGG | 3 cut(s) | 561, 1308, 1356 |
| Bme18I | GGWCC | 2 cut(s) | 61, 1313 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1392 |
| BmgT120I | GGNCC | 4 cut(s) | 61, 214, 1275, 1313 |
| BmiI | GGNNCC | 4 cut(s) | 7, 545, 735, 1075 |
| BmrFI | CCNGG | 3 cut(s) | 561, 1308, 1356 |
| BmrI | ACTGGG | 3 cut(s) | 388, 904, 1880 |
| BmsI | GCATC | 5 cut(s) | 100, 430, 462, 661, 1006 |
| BmuI | ACTGGG | 3 cut(s) | 388, 904, 1880 |
| BpiI | GAAGAC | 2 cut(s) | 180, 377 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1018, 1050, 1668, 1700 |
| BpmI | CTGGAG | 2 cut(s) | 557, 1092 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1320 |
| Bpu14I | TTCGAA | 1 cut(s) | 1758 |
| BpuEI | CTTGAG | 2 cut(s) | 200, 965 |
| BpuMI | CCSGG | 2 cut(s) | 561, 1308 |
| BsaHI | GRCGYC | 1 cut(s) | 1393 |
| BsaI | GGTCTC | 1 cut(s) | 1297 |
| BsaWI | WCCGGW | 1 cut(s) | 546 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 689, 1355, 1477 |
| Bse118I | RCCGGY | 1 cut(s) | 546 |
| Bse1I | ACTGG | 8 cut(s) | 38, 383, 540, 710, 899, 1722, 1815, 1886 |
| Bse3DI | GCAATG | 1 cut(s) | 1446 |
| BseBI | CCWGG | 1 cut(s) | 1356 |
| BseGI | GGATG | 7 cut(s) | 9, 136, 265, 676, 1054, 1160, 1994 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 689, 1355, 1477 |
| BseMI | GCAATG | 1 cut(s) | 1446 |
| BseMII | CTCAG | 5 cut(s) | 330, 459, 1404, 1466, 1821 |
| BseNI | ACTGG | 8 cut(s) | 38, 383, 540, 710, 899, 1722, 1815, 1886 |
| BseSI | GKGCMC | 2 cut(s) | 300, 937 |
| BseXI | GCAGC | 5 cut(s) | 211, 718, 850, 1006, 1108 |
| BseYI | CCCAGC | 1 cut(s) | 1868 |
| BsgI | GTGCAG | 1 cut(s) | 1683 |
| Bsh1236I | CGCG | 1 cut(s) | 871 |
| BshFI | GGCC | 7 cut(s) | 215, 577, 1053, 1276, 1721, 1828, 1971 |
| BshNI | GGYRCC | 1 cut(s) | 543 |
| BshTI | ACCGGT | 1 cut(s) | 546 |
| BsiHKAI | GWGCWC | 4 cut(s) | 95, 300, 917, 937 |
| BsiSI | CCGG | 3 cut(s) | 547, 560, 1307 |
| BslFI | GGGAC | 5 cut(s) | 415, 1412, 1654, 1793, 1821 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 689, 1355, 1477 |
| BsmAI | GTCTC | 5 cut(s) | 1297, 1379, 1544, 1569, 1826 |
| BsmBI | CGTCTC | 1 cut(s) | 1379 |
| BsmFI | GGGAC | 5 cut(s) | 415, 1412, 1654, 1793, 1821 |
| BsmI | GAATGC | 1 cut(s) | 997 |
| BsnI | GGCC | 7 cut(s) | 215, 577, 1053, 1276, 1721, 1828, 1971 |
| Bso31I | GGTCTC | 1 cut(s) | 1297 |
| Bsp119I | TTCGAA | 1 cut(s) | 1758 |
| Bsp1286I | GDGCHC | 4 cut(s) | 95, 300, 917, 937 |
| Bsp143I | GATC | 6 cut(s) | 388, 568, 638, 857, 1480, 1593 |
| BspACI | CCGC | 3 cut(s) | 737, 1046, 1747 |
| BspANI | GGCC | 7 cut(s) | 215, 577, 1053, 1276, 1721, 1828, 1971 |
| BspCNI | CTCAG | 5 cut(s) | 329, 460, 1405, 1465, 1822 |
| BspFNI | CGCG | 1 cut(s) | 871 |
| BspHI | TCATGA | 1 cut(s) | 778 |
| BspLI | GGNNCC | 4 cut(s) | 7, 545, 735, 1075 |
| BspMAI | CTGCAG | 1 cut(s) | 1986 |
| BspMI | ACCTGC | 1 cut(s) | 1840 |
| BspPI | GGATC | 3 cut(s) | 646, 865, 1488 |
| BspT104I | TTCGAA | 1 cut(s) | 1758 |
| BspT107I | GGYRCC | 1 cut(s) | 543 |
| BspTNI | GGTCTC | 1 cut(s) | 1297 |
| BsrDI | GCAATG | 1 cut(s) | 1446 |
| BsrFI | RCCGGY | 1 cut(s) | 546 |
| BsrI | ACTGG | 8 cut(s) | 38, 383, 540, 710, 899, 1722, 1815, 1886 |
| BssAI | RCCGGY | 1 cut(s) | 546 |
| BssMI | GATC | 6 cut(s) | 388, 568, 638, 857, 1480, 1593 |
| BssNI | GRCGYC | 1 cut(s) | 1393 |
| BssSI | CACGAG | 1 cut(s) | 1731 |
| Bst2BI | CACGAG | 1 cut(s) | 1731 |
| Bst2UI | CCWGG | 1 cut(s) | 1356 |
| Bst4CI | ACNGT | 7 cut(s) | 209, 820, 921, 1177, 1555, 1671, 1704 |
| Bst6I | CTCTTC | 3 cut(s) | 1377, 1691, 1910 |
| BstACI | GRCGYC | 1 cut(s) | 1393 |
| BstBI | TTCGAA | 1 cut(s) | 1758 |
| BstC8I | GCNNGC | 3 cut(s) | 511, 949, 1063 |
| BstDEI | CTNAG | 7 cut(s) | 68, 316, 468, 1320, 1413, 1452, 1830 |
| BstF5I | GGATG | 7 cut(s) | 9, 136, 265, 676, 1054, 1160, 1994 |
| BstFNI | CGCG | 1 cut(s) | 871 |
| BstHHI | GCGC | 2 cut(s) | 843, 871 |
| BstKTI | GATC | 6 cut(s) | 391, 571, 641, 860, 1483, 1596 |
| BstMAI | GTCTC | 5 cut(s) | 1297, 1379, 1544, 1569, 1826 |
| BstMBI | GATC | 6 cut(s) | 388, 568, 638, 857, 1480, 1593 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 221, 354, 847, 991, 1067, 1223 |
| BstNI | CCWGG | 1 cut(s) | 1356 |
| BstNSI | RCATGY | 2 cut(s) | 199, 410 |
| BstSCI | CCNGG | 3 cut(s) | 559, 1306, 1354 |
| BstSFI | CTRYAG | 2 cut(s) | 1368, 1982 |
| BstSLI | GKGCMC | 2 cut(s) | 300, 937 |
| BstUI | CGCG | 1 cut(s) | 871 |
| BstV1I | GCAGC | 5 cut(s) | 211, 718, 850, 1006, 1108 |
| BstV2I | GAAGAC | 2 cut(s) | 180, 377 |
| BstX2I | RGATCY | 2 cut(s) | 857, 1480 |
| BstYI | RGATCY | 2 cut(s) | 857, 1480 |
| BsuRI | GGCC | 7 cut(s) | 215, 577, 1053, 1276, 1721, 1828, 1971 |
| BtsCI | GGATG | 7 cut(s) | 9, 136, 265, 676, 1054, 1160, 1994 |
| BtsI | GCAGTG | 1 cut(s) | 1850 |
| BtsIMutI | CAGTG | 6 cut(s) | 578, 917, 1560, 1700, 1729, 1850 |
| BveI | ACCTGC | 1 cut(s) | 1840 |
| Cac8I | GCNNGC | 3 cut(s) | 511, 949, 1063 |
| CaiI | CAGNNNCTG | 2 cut(s) | 443, 1124 |
| CciI | TCATGA | 1 cut(s) | 778 |
| CfoI | GCGC | 2 cut(s) | 843, 871 |
| Cfr10I | RCCGGY | 1 cut(s) | 546 |
| Cfr13I | GGNCC | 4 cut(s) | 61, 214, 1275, 1313 |
| CseI | GACGC | 1 cut(s) | 1382 |
| CsiI | ACCWGGT | 1 cut(s) | 1354 |
| Csp6I | GTAC | 5 cut(s) | 130, 151, 544, 585, 1438 |
| CspAI | ACCGGT | 1 cut(s) | 546 |
| CviAII | CATG | 6 cut(s) | 196, 407, 770, 779, 1238, 1625 |
| CviQI | GTAC | 5 cut(s) | 130, 151, 544, 585, 1438 |
| DdeI | CTNAG | 7 cut(s) | 68, 316, 468, 1320, 1413, 1452, 1830 |
| DpnI | GATC | 6 cut(s) | 390, 570, 640, 859, 1482, 1595 |
| DpnII | GATC | 6 cut(s) | 388, 568, 638, 857, 1480, 1593 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1392 |
| EaeI | YGGCCR | 1 cut(s) | 1969 |
| Eam1104I | CTCTTC | 3 cut(s) | 1377, 1691, 1910 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1392 |
| EarI | CTCTTC | 3 cut(s) | 1377, 1691, 1910 |
| Ecl136II | GAGCTC | 1 cut(s) | 93 |
| Eco147I | AGGCCT | 1 cut(s) | 1828 |
| Eco24I | GRGCYC | 1 cut(s) | 95 |
| Eco31I | GGTCTC | 1 cut(s) | 1297 |
| Eco32I | GATATC | 1 cut(s) | 1116 |
| Eco47I | GGWCC | 2 cut(s) | 61, 1313 |
| Eco53kI | GAGCTC | 1 cut(s) | 93 |
| EcoICRI | GAGCTC | 1 cut(s) | 93 |
| EcoRII | CCWGG | 1 cut(s) | 1354 |
| EcoRV | GATATC | 1 cut(s) | 1116 |
| EcoT38I | GRGCYC | 1 cut(s) | 95 |
| Esp3I | CGTCTC | 1 cut(s) | 1379 |
| FaeI | CATG | 6 cut(s) | 199, 410, 773, 782, 1241, 1628 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 162, 194 |
| FaqI | GGGAC | 5 cut(s) | 415, 1412, 1654, 1793, 1821 |
| FatI | CATG | 6 cut(s) | 195, 406, 769, 778, 1237, 1624 |
| FbaI | TGATCA | 2 cut(s) | 568, 1593 |
| FblI | GTMKAC | 1 cut(s) | 1890 |
| Fnu4HI | GCNGC | 7 cut(s) | 225, 707, 737, 839, 995, 1122, 1748 |
| FokI | GGATG | 6 cut(s) | 123, 252, 683, 1061, 1147, 2001 |
| FriOI | GRGCYC | 1 cut(s) | 95 |
| Fsp4HI | GCNGC | 7 cut(s) | 225, 707, 737, 839, 995, 1122, 1748 |
| FspBI | CTAG | 6 cut(s) | 167, 902, 1055, 1214, 1230, 2035 |
| GlaI | GCGC | 2 cut(s) | 842, 870 |
| GluI | GCNGC | 7 cut(s) | 225, 707, 737, 839, 995, 1122, 1748 |
| GsaI | CCCAGC | 1 cut(s) | 1872 |
| GsuI | CTGGAG | 2 cut(s) | 557, 1092 |
| HaeIII | GGCC | 7 cut(s) | 215, 577, 1053, 1276, 1721, 1828, 1971 |
| HapII | CCGG | 3 cut(s) | 547, 560, 1307 |
| HgaI | GACGC | 1 cut(s) | 1382 |
| HhaI | GCGC | 2 cut(s) | 843, 871 |
| Hin1I | GRCGYC | 1 cut(s) | 1393 |
| Hin1II | CATG | 6 cut(s) | 199, 410, 773, 782, 1241, 1628 |
| Hin6I | GCGC | 2 cut(s) | 841, 869 |
| HinP1I | GCGC | 2 cut(s) | 841, 869 |
| HincII | GTYRAC | 2 cut(s) | 630, 924 |
| HindII | GTYRAC | 2 cut(s) | 630, 924 |
| HindIII | AAGCTT | 4 cut(s) | 304, 399, 1137, 1322 |
| HinfI | GANTC | 9 cut(s) | 26, 74, 326, 458, 728, 775, 788, 806, 1978 |
| HpaI | GTTAAC | 1 cut(s) | 924 |
| HpaII | CCGG | 3 cut(s) | 547, 560, 1307 |
| HphI | GGTGA | 5 cut(s) | 264, 1025, 1516, 1573, 1644 |
| Hpy166II | GTNNAC | 8 cut(s) | 298, 630, 924, 935, 974, 1313, 1564, 1891 |
| Hpy8I | GTNNAC | 8 cut(s) | 298, 630, 924, 935, 974, 1313, 1564, 1891 |
| Hpy99I | CGWCG | 1 cut(s) | 1398 |
| HpyAV | CCTTC | 6 cut(s) | 279, 530, 588, 683, 1666, 2029 |
| HpyCH4III | ACNGT | 7 cut(s) | 209, 820, 921, 1177, 1555, 1671, 1704 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 221, 354, 847, 991, 1067, 1223 |
| HpyF3I | CTNAG | 7 cut(s) | 68, 316, 468, 1320, 1413, 1452, 1830 |
| Hsp92I | GRCGYC | 1 cut(s) | 1393 |
| Hsp92II | CATG | 6 cut(s) | 199, 410, 773, 782, 1241, 1628 |
| HspAI | GCGC | 2 cut(s) | 841, 869 |
| KpnI | GGTACC | 1 cut(s) | 547 |
| Ksp22I | TGATCA | 2 cut(s) | 568, 1593 |
| KspAI | GTTAAC | 1 cut(s) | 924 |
| Kzo9I | GATC | 6 cut(s) | 388, 568, 638, 857, 1480, 1593 |
| LmnI | GCTCC | 2 cut(s) | 733, 1073 |
| Lsp1109I | GCAGC | 5 cut(s) | 211, 718, 850, 1006, 1108 |
| LweI | GCATC | 5 cut(s) | 100, 430, 462, 661, 1006 |
| MabI | ACCWGGT | 1 cut(s) | 1354 |
| MaeI | CTAG | 6 cut(s) | 167, 902, 1055, 1214, 1230, 2035 |
| MaeIII | GTNAC | 3 cut(s) | 1153, 1179, 1663 |
| MalI | GATC | 6 cut(s) | 390, 570, 640, 859, 1482, 1595 |
| MboI | GATC | 6 cut(s) | 388, 568, 638, 857, 1480, 1593 |
| MfeI | CAATTG | 2 cut(s) | 1338, 1516 |
| MflI | RGATCY | 2 cut(s) | 857, 1480 |
| MhlI | GDGCHC | 4 cut(s) | 95, 300, 917, 937 |
| MlsI | TGGCCA | 1 cut(s) | 1971 |
| MluCI | AATT | 9 cut(s) | 55, 1203, 1338, 1472, 1488, 1516, 1602, 1648, 1920 |
| MluNI | TGGCCA | 1 cut(s) | 1971 |
| MlyI | GAGTC | 2 cut(s) | 722, 797 |
| MmeI | TCCRAC | 2 cut(s) | 1312, 1387 |
| Mox20I | TGGCCA | 1 cut(s) | 1971 |
| MscI | TGGCCA | 1 cut(s) | 1971 |
| MseI | TTAA | 8 cut(s) | 147, 252, 551, 923, 1133, 1422, 1485, 1605 |
| MslI | CAYNNNNRTG | 2 cut(s) | 69, 596 |
| Msp20I | TGGCCA | 1 cut(s) | 1971 |
| MspA1I | CMGCKG | 1 cut(s) | 1121 |
| MspI | CCGG | 3 cut(s) | 547, 560, 1307 |
| MspR9I | CCNGG | 3 cut(s) | 561, 1308, 1356 |
| MunI | CAATTG | 2 cut(s) | 1338, 1516 |
| Mva1269I | GAATGC | 1 cut(s) | 997 |
| MvaI | CCWGG | 1 cut(s) | 1356 |
| MvnI | CGCG | 1 cut(s) | 871 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 221, 354, 847, 991, 1067, 1223 |
| NciI | CCSGG | 2 cut(s) | 561, 1308 |
| NdeII | GATC | 6 cut(s) | 388, 568, 638, 857, 1480, 1593 |
| NlaIII | CATG | 6 cut(s) | 199, 410, 773, 782, 1241, 1628 |
| NlaIV | GGNNCC | 4 cut(s) | 7, 545, 735, 1075 |
| NmuCI | GTSAC | 2 cut(s) | 1179, 1663 |
| NspI | RCATGY | 2 cut(s) | 199, 410 |
| NspV | TTCGAA | 1 cut(s) | 1758 |
| OliI | CACNNNNGTG | 1 cut(s) | 69 |
| PagI | TCATGA | 1 cut(s) | 778 |
| PceI | AGGCCT | 1 cut(s) | 1828 |
| PciI | ACATGT | 1 cut(s) | 406 |
| PctI | GAATGC | 1 cut(s) | 997 |
| PfeI | GAWTC | 7 cut(s) | 26, 74, 326, 458, 775, 806, 1978 |
| PflFI | GACNNNGTC | 2 cut(s) | 1177, 1580 |
| PinAI | ACCGGT | 1 cut(s) | 546 |
| PkrI | GCNGC | 7 cut(s) | 226, 708, 738, 840, 996, 1123, 1749 |
| PleI | GAGTC | 2 cut(s) | 722, 796 |
| PpsI | GAGTC | 2 cut(s) | 722, 796 |
| PscI | ACATGT | 1 cut(s) | 406 |
| PshBI | ATTAAT | 1 cut(s) | 1605 |
| Psp124BI | GAGCTC | 1 cut(s) | 95 |
| Psp6I | CCWGG | 1 cut(s) | 1354 |
| PspFI | CCCAGC | 1 cut(s) | 1868 |
| PspGI | CCWGG | 1 cut(s) | 1354 |
| PspN4I | GGNNCC | 4 cut(s) | 7, 545, 735, 1075 |
| PspPI | GGNCC | 4 cut(s) | 61, 214, 1275, 1313 |
| PstI | CTGCAG | 1 cut(s) | 1986 |
| PstNI | CAGNNNCTG | 2 cut(s) | 443, 1124 |
| PsuI | RGATCY | 2 cut(s) | 857, 1480 |
| PsyI | GACNNNGTC | 2 cut(s) | 1177, 1580 |
| PvuII | CAGCTG | 1 cut(s) | 1121 |
| RsaI | GTAC | 5 cut(s) | 131, 152, 545, 586, 1439 |
| RsaNI | GTAC | 5 cut(s) | 130, 151, 544, 585, 1438 |
| RseI | CAYNNNNRTG | 2 cut(s) | 69, 596 |
| SacI | GAGCTC | 1 cut(s) | 95 |
| SaqAI | TTAA | 8 cut(s) | 147, 252, 551, 923, 1133, 1422, 1485, 1605 |
| SatI | GCNGC | 7 cut(s) | 225, 707, 737, 839, 995, 1122, 1748 |
| Sau3AI | GATC | 6 cut(s) | 388, 568, 638, 857, 1480, 1593 |
| Sau96I | GGNCC | 4 cut(s) | 61, 214, 1275, 1313 |
| ScaI | AGTACT | 1 cut(s) | 586 |
| SchI | GAGTC | 2 cut(s) | 722, 797 |
| ScrFI | CCNGG | 3 cut(s) | 561, 1308, 1356 |
| SduI | GDGCHC | 4 cut(s) | 95, 300, 917, 937 |
| SexAI | ACCWGGT | 1 cut(s) | 1354 |
| SfaNI | GCATC | 5 cut(s) | 100, 430, 462, 661, 1006 |
| SfcI | CTRYAG | 2 cut(s) | 1368, 1982 |
| SfuI | TTCGAA | 1 cut(s) | 1758 |
| SinI | GGWCC | 2 cut(s) | 61, 1313 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 69, 596 |
| SmlI | CTYRAG | 2 cut(s) | 179, 980 |
| SmoI | CTYRAG | 2 cut(s) | 179, 980 |
| SpeI | ACTAGT | 1 cut(s) | 901 |
| Sse9I | AATT | 9 cut(s) | 55, 1203, 1338, 1472, 1488, 1516, 1602, 1648, 1920 |
| SseBI | AGGCCT | 1 cut(s) | 1828 |
| SsiI | CCGC | 3 cut(s) | 737, 1046, 1747 |
| SspMI | CTAG | 6 cut(s) | 167, 902, 1055, 1214, 1230, 2035 |
| SstI | GAGCTC | 1 cut(s) | 95 |
| StuI | AGGCCT | 1 cut(s) | 1828 |
| StyD4I | CCNGG | 3 cut(s) | 559, 1306, 1354 |
| TaaI | ACNGT | 7 cut(s) | 209, 820, 921, 1177, 1555, 1671, 1704 |
| TaqI | TCGA | 1 cut(s) | 1758 |
| TasI | AATT | 9 cut(s) | 55, 1203, 1338, 1472, 1488, 1516, 1602, 1648, 1920 |
| TatI | WGTACW | 4 cut(s) | 129, 150, 584, 1437 |
| TauI | GCSGC | 2 cut(s) | 739, 1750 |
| TfiI | GAWTC | 7 cut(s) | 26, 74, 326, 458, 775, 806, 1978 |
| Tru1I | TTAA | 8 cut(s) | 147, 252, 551, 923, 1133, 1422, 1485, 1605 |
| Tru9I | TTAA | 8 cut(s) | 147, 252, 551, 923, 1133, 1422, 1485, 1605 |
| TscAI | CASTG | 6 cut(s) | 578, 924, 1560, 1707, 1729, 1850 |
| TseFI | GTSAC | 2 cut(s) | 1179, 1663 |
| TseI | GCWGC | 5 cut(s) | 224, 706, 838, 994, 1121 |
| Tsp45I | GTSAC | 2 cut(s) | 1179, 1663 |
| TspDTI | ATGAA | 9 cut(s) | 819, 1149, 1226, 1538, 1609, 1613, 1755, 1928, 1977 |
| TspRI | CASTG | 6 cut(s) | 578, 924, 1560, 1707, 1729, 1850 |
| Tth111I | GACNNNGTC | 2 cut(s) | 1177, 1580 |
| VneI | GTGCAC | 2 cut(s) | 296, 933 |
| VpaK11BI | GGWCC | 2 cut(s) | 61, 1313 |
| VspI | ATTAAT | 1 cut(s) | 1605 |
| XapI | RAATTY | 1 cut(s) | 1920 |
| XbaI | TCTAGA | 1 cut(s) | 166 |
| XceI | RCATGY | 2 cut(s) | 199, 410 |
| XmiI | GTMKAC | 1 cut(s) | 1890 |
| XspI | CTAG | 6 cut(s) | 167, 902, 1055, 1214, 1230, 2035 |
| ZrmI | AGTACT | 1 cut(s) | 586 |