FvH4_5g24540

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000280
Physical Location & Seq
Forward (+)
15896777 .. 15906893
10117 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g24540.t2

Sequence Viewer

Length: 3003 bp
ATGGCCAGACGAGGAAAGAGGAAGAGGAAGCAAGACCCAGACACCCATCTCCCAAATCAAACTGAACATGTTGCTGTCAAAGCTGGGGATGAGGTTCTTTCTGAGATAAAGGGTGAGGACAGTTTGGGGAATACTCAAAAGGAAGAAAAAGCTGAAGGGGTTGGTGTTGGTGTGAAGCAGAAGAAGAAGCCTGGAAGGAAGAGTAAGAAGGGTTTGGTGGGGAATGGAGATGGGGGTCTAAAGGGGGTTGTTGGTTTTGGTGGTGGAGAGATTGCTGTGAGGACTTTGAGGAAGAGGCCTGAACGGAACAAGAAGGTTGGAGATATGGAGGCAGCCGGAAATGGGGAGAAAGGTAAGGAGAATGGTGCTAAGTCTGTGAAGAAGCGTGGCGGGGAAGGTAGGAAAAAGGTGGAAATTGCGAAGGAAGAAGGGAAGAAAGGTGAATTAGAAGTGAAGGGTGACACCAAACGCGGTAGGAAGCGTGCGAAAACTGAAGAAGGTGGGAATAAGGTAAAGGTACGTTGTTCATTTAGGCGTCTGAGGGAGCAAGGACATCTATCAGCGGAGGAGCGTAAAAAGTGGATTGAGGAGGTGTCCATGATGTGCCACCAATGTCAGAGGAGTGATAAGGGAAGCGTTGTTCGATGCAAAAAGTGCAAGACAAAGCGATATTGTGTTCCTTGCATAGAGAATTGGTATCCACATACGTCAGAGGATGCCATCGCTGGGGCTTGTCCTTTTTGCTGTGGAAATTGCAACTGCAAAGCATGCTTGCGCTTAGATATACCTGTTAAGAATTTGAAGAACCAAGAATTAGTACTTGACAAAAATGTAAGAGTTGAGCACTCTAAGTACTTAATTCATGCACTGCTTCCATCCTTGAAAATAATTCTTGATGAACAAAAGAGTGAGATGGCAGAGGAGGCAAGGAGCAAAGGAATATCTGACTCTGAGCTCCAGATACCAAAGTCTGATTGCACTGCTGATGTTCGTGTATATTGCAACAACTGCAGAACTTCTATTTTTGATTATCACAGAAGCTGTCCTAAATGTTCATATGACCTCTGCCTCCTTTGTTGCCTGGAGATTCGAGAAGGAAAATTACAGTTAGGTACGGAGGAAGTGACTCTGGAGTATGTTTCTCAGGGCCTACCATACTTGCATGGTGGAGAGGAAGCGAAAAACAAAAACGGAAAGGAAAAGAGAAATAGGAATGCTGTGGAGCCACCCACTGAAACTGGCCCCAAAACTTCTGGAAGGCGAATATTTGAGTGGAAATCAGATGAAGATGGAATCATCCTTTGCCCCCCAGAGGACATGGGAGGTTGTGGTGTTGGTAAGTTAGAATTGAAATGCATCTTTTCTAAAAATTATGTCGAAGAGTTGGTAAAGAAAGCAGAAGTGATAGATGAAACTTACAAACTAGTGTATACAAGTGGAACTTCTGCAGAGAGGTGTCCATGTCTCAACTCTGTGGACGATGTCAATATAAATATCAATACGTCAAGAAAAGCAGCTTCTCGAGACAATTCTGAGGACAACTATCTGTACTGTGCAAGGGCTGGAGATATTAAAGTAGAGGATGTAAAGCATTTCCAGTGGCATTGGATCAAGGGTGAGCCTGTTATCATTAGCAATGCACTTGAAACTGGATCGGGTTTAAGTTGGGAACCATTGGTCATGTGGCGTGCTTGTCGTCAGATGCAACATACTAAGCATGGTAAACATTTGAACGTCAAAGCAATAGATTGCTTGGATTGGTGTGAGGCGGAGGTCAATATCCACTGGTTTTTTACTGGATACTTGAAGGGATGGTTTGATCAAGAGCATTGGCCTCAGATATTGAAACTGAAAGACTGGCCACCAACTAATTTATTTGAGGAGCGCCTTCCTCGTCATGGTGCTGAGTTCATTAGTTGTCTACCATTTAAGGAATATACTCATCCTCGCAATGGTCCTCTGAACCTTTTTGCCAAATTACCTTCAGAATGTGTAAAACCAGACATGGGACCAAAGACATATATTGCTTATGGTTTTTCCCAGGAGCTTGGACGTGGAGATTCTGTAACCAAGCTTCATTGTGACATGTCTGATGCGGTGAATATTTTGACACATACCACTGAAGTGAATCTTACTCCGGAGCAACTTACTATCATTGAAAAGTTGAAAAAGAAGCATGCTATTCAAGACCGAAGAGAAATTTTTGGCAATTGTCAGATTGTTGGTGATAATATTGATAGTGACAAGGCTGGTGGTGATTCTTTCTTCAGTGCTGCTGATGATAAACAAACTTTTAATGAAGTTGAAAGTAATAGCATTGGTGTAGCTGTTTCAAAACTAGTTGATGCGATTGTTCCTCAAGTTGGAGATGCTTCAATGGGATTCGAATCTGAATCTGAAATGAATGAAGAGGCAGGGTTACATCGTGATATAGGTAGTGAAATGCCTGCAAGAAATTATGGAAATAAGTTGAAAGAGTCAGATGTCACTGAGGGAGGTGCTTTATGGGACATATTCCGTAGACAGGATGTTCCTAAGCTGCAGGAGTATCTCATGAAGCATTTCAAGGAATTCAGGCACATACACTGTTGCCCAGTGCAGCAGGTTGTCCATCCCATATATGATCAGACATTTTATCTTACAGTGGAGCACAAAAAGAAGCTTAAAGAGGAATATGGAATCGAACCGTGGACATTCATTCAAAAACTTGGAGATGCTGTCTTTATTCCTGCAGGATGTCCGCATCAAGTTAGGAATTTGAAGTCATGCATAAAAGTTGCAATGGACTTTGTCTCTCCTGAAAATGTCGGCGAGTGCTTTCACTTGACAGAAGTATTCCGCACACTTCCAGAAGATCATAGGGCTAAAGAGGACAAATTGGAGGTAAAGAAAATGATTGTACATTCTGTCACTGATGCTCTGAAGGAACTTGATCAGATTGCGAGGTCTGAGGAAGACACTAGTCCACCTAAATTAAGTGAGAGGATGCAGTATACTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1001

Amino Acids

112.85

Weight (kDa)

8.05

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjC PF02373 834 - 930 1.4e-13 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2593
AccI GTMKAC 4 cut(s) 1430, 1921, 2521, 2993
AccII CGCG 1 cut(s) 471
AccIII TCCGGA 1 cut(s) 2137
AciI CCGC 7 cut(s) 390, 471, 563, 1769, 2096, 2741, 2839
AclWI GGATC 2 cut(s) 1616, 1660
AcoI YGGCCR 2 cut(s) 3, 1859
AcsI RAATTY 4 cut(s) 796, 2199, 2570, 2755
AcuI CTGAAG 6 cut(s) 174, 513, 1968, 2142, 2251, 2942
AcyI GRCGYC 1 cut(s) 535
AfaI GTAC 6 cut(s) 519, 819, 854, 1114, 1550, 2901
AfiI CCNNNNNNNGG 8 cut(s) 342, 726, 1312, 1898, 1952, 2006, 2363, 2524
AflIII ACRYGT 2 cut(s) 67, 2085
AhlI ACTAGT 3 cut(s) 1423, 2338, 2960
AjiI CACGTC 1 cut(s) 2054
AjnI CCWGG 3 cut(s) 190, 1080, 2040
AleI CACNNNNGTG 1 cut(s) 2123
AloI GAACNNNNNNTCC 2 cut(s) 624, 656
Alw21I GWGCWC 3 cut(s) 846, 957, 2652
Alw26I GTCTC 3 cut(s) 1469, 1518, 2797
AlwI GGATC 2 cut(s) 1616, 1660
AlwNI CAGNNNCTG 1 cut(s) 1041
Ama87I CYCGRG 1 cut(s) 1521
Aor13HI TCCGGA 1 cut(s) 2137
AoxI GGCC 6 cut(s) 3, 296, 1147, 1240, 1832, 1859
ApeKI GCWGC 5 cut(s) 332, 1514, 2273, 2539, 2599
ApoI RAATTY 4 cut(s) 796, 2199, 2570, 2755
Asp700I GAANNNNTTC 1 cut(s) 2561
AspLEI GCGC 2 cut(s) 777, 1887
AspS9I GGNCC 4 cut(s) 1147, 1241, 1955, 2009
AsuHPI GGTGA 7 cut(s) 125, 452, 470, 1628, 2110, 2237, 2267
AsuII TTCGAA 1 cut(s) 2385
AvaI CYCGRG 1 cut(s) 1521
AvaII GGWCC 2 cut(s) 1955, 2009
BaeI ACNNNNGTAYC 2 cut(s) 953, 986
BalI TGGCCA 2 cut(s) 5, 1861
BanII GRGCYC 1 cut(s) 957
BarI GAAGNNNNNNTAC 4 cut(s) 1086, 1118, 2058, 2090
BbsI GAAGAC 1 cut(s) 2961
Bbv12I GWGCWC 3 cut(s) 846, 957, 2652
BbvI GCAGC 5 cut(s) 344, 1526, 2260, 2526, 2611
BccI CCATC 8 cut(s) 54, 224, 728, 883, 907, 1283, 1806, 2619
BciT130I CCWGG 3 cut(s) 192, 1082, 2042
BciVI GTATCC 2 cut(s) 708, 1793
BclI TGATCA 3 cut(s) 1819, 2623, 2932
BcoDI GTCTC 3 cut(s) 1469, 1518, 2797
BcuI ACTAGT 3 cut(s) 1423, 2338, 2960
BfaI CTAG 3 cut(s) 1424, 2339, 2961
BfmI CTRYAG 4 cut(s) 1009, 1446, 2540, 2730
BfoI RGCGCY 1 cut(s) 1888
BfuAI ACCTGC 1 cut(s) 2593
BfuI GTATCC 2 cut(s) 708, 1793
BisI GCNGC 5 cut(s) 333, 1515, 2274, 2540, 2600
BlsI GCNGC 5 cut(s) 334, 1516, 2275, 2541, 2601
BmcAI AGTACT 2 cut(s) 819, 854
Bme1390I CCNGG 3 cut(s) 192, 1082, 2042
Bme18I GGWCC 2 cut(s) 1955, 2009
BmeT110I CYCGRG 1 cut(s) 1521
BmgBI CACGTC 1 cut(s) 2054
BmgT120I GGNCC 4 cut(s) 1147, 1241, 1955, 2009
BmiI GGNNCC 4 cut(s) 1224, 1243, 1671, 2010
BmrFI CCNGG 3 cut(s) 192, 1082, 2042
BmrI ACTGGG 1 cut(s) 2588
BmuI ACTGGG 1 cut(s) 2588
BoxI GACNNNNGTC 1 cut(s) 2961
BpiI GAAGAC 1 cut(s) 2961
BpmI CTGGAG 4 cut(s) 941, 1103, 1151, 1584
Bpu10I CCTNAGC 1 cut(s) 2535
Bpu14I TTCGAA 1 cut(s) 2385
BpuEI CTTGAG 1 cut(s) 2343
BsaBI GATNNNNATC 1 cut(s) 2386
BsaHI GRCGYC 1 cut(s) 535
BsaJI CCNNGG 2 cut(s) 2040, 2687
BsaWI WCCGGW 1 cut(s) 2137
BsaXI ACNNNNNCTCC 4 cut(s) 2049, 2079, 2703, 2733
Bsc4I CCNNNNNNNGG 8 cut(s) 342, 726, 1312, 1898, 1952, 2006, 2363, 2524
Bse1I ACTGG 7 cut(s) 1243, 1597, 1654, 1790, 1801, 1862, 2594
Bse3DI GCAATG 3 cut(s) 1642, 1957, 2787
Bse8I GATNNNNATC 1 cut(s) 2386
BseAI TCCGGA 1 cut(s) 2137
BseBI CCWGG 3 cut(s) 192, 1082, 2042
BseDI CCNNGG 2 cut(s) 2040, 2687
BseJI GATNNNNATC 1 cut(s) 2386
BseLI CCNNNNNNNGG 8 cut(s) 342, 726, 1312, 1898, 1952, 2006, 2363, 2524
BseMI GCAATG 3 cut(s) 1642, 1957, 2787
BseMII CTCAG 9 cut(s) 93, 530, 942, 1157, 1524, 1850, 1896, 2481, 2940
BseNI ACTGG 7 cut(s) 1243, 1597, 1654, 1790, 1801, 1862, 2594
BseRI GAGGAG 5 cut(s) 581, 602, 634, 935, 1895
BseXI GCAGC 5 cut(s) 344, 1526, 2260, 2526, 2611
BseYI CCCAGC 2 cut(s) 83, 725
BsgI GTGCAG 1 cut(s) 2618
Bsh1236I CGCG 1 cut(s) 471
BshFI GGCC 6 cut(s) 5, 298, 1149, 1242, 1834, 1861
BsiHKAI GWGCWC 3 cut(s) 846, 957, 2652
BsiHKCI CYCGRG 1 cut(s) 1521
BsiSI CCGG 2 cut(s) 336, 2138
BslFI GGGAC 2 cut(s) 2022, 2522
BslI CCNNNNNNNGG 8 cut(s) 342, 726, 1312, 1898, 1952, 2006, 2363, 2524
BsmAI GTCTC 3 cut(s) 1469, 1518, 2797
BsmFI GGGAC 2 cut(s) 2022, 2522
BsmI GAATGC 1 cut(s) 1219
BsnI GGCC 6 cut(s) 5, 298, 1149, 1242, 1834, 1861
BsoBI CYCGRG 1 cut(s) 1521
Bsp119I TTCGAA 1 cut(s) 2385
Bsp1286I GDGCHC 3 cut(s) 846, 957, 2652
Bsp13I TCCGGA 1 cut(s) 2137
Bsp1407I TGTACA 1 cut(s) 2899
Bsp143I GATC 6 cut(s) 1608, 1652, 1819, 2623, 2854, 2932
BspACI CCGC 7 cut(s) 390, 471, 563, 1769, 2096, 2741, 2839
BspANI GGCC 6 cut(s) 5, 298, 1149, 1242, 1834, 1861
BspCNI CTCAG 9 cut(s) 94, 531, 943, 1156, 1525, 1849, 1897, 2482, 2941
BspEI TCCGGA 1 cut(s) 2137
BspFNI CGCG 1 cut(s) 471
BspHI TCATGA 1 cut(s) 2553
BspLI GGNNCC 4 cut(s) 1224, 1243, 1671, 2010
BspMAI CTGCAG 4 cut(s) 1013, 1450, 2544, 2734
BspMI ACCTGC 1 cut(s) 2593
BspPI GGATC 2 cut(s) 1616, 1660
BspT104I TTCGAA 1 cut(s) 2385
BsrDI GCAATG 3 cut(s) 1642, 1957, 2787
BsrGI TGTACA 1 cut(s) 2899
BsrI ACTGG 7 cut(s) 1243, 1597, 1654, 1790, 1801, 1862, 2594
BssECI CCNNGG 2 cut(s) 2040, 2687
BssMI GATC 6 cut(s) 1608, 1652, 1819, 2623, 2854, 2932
BssNAI GTATAC 2 cut(s) 1431, 2994
BssNI GRCGYC 1 cut(s) 535
Bst1107I GTATAC 2 cut(s) 1431, 2994
Bst2UI CCWGG 3 cut(s) 192, 1082, 2042
Bst4CI ACNGT 6 cut(s) 122, 1107, 1553, 2588, 2644, 2688
Bst6I CTCTTC 6 cut(s) 17, 194, 287, 1374, 2188, 2403
BstACI GRCGYC 1 cut(s) 535
BstAPI GCANNNNNTGC 3 cut(s) 654, 768, 1008
BstAUI TGTACA 1 cut(s) 2899
BstBI TTCGAA 1 cut(s) 2385
BstC8I GCNNGC 6 cut(s) 483, 769, 773, 1689, 2178, 2448
BstDSI CCRYGG 1 cut(s) 2687
BstFNI CGCG 1 cut(s) 471
BstH2I RGCGCY 1 cut(s) 1888
BstHHI GCGC 2 cut(s) 777, 1887
BstKTI GATC 6 cut(s) 1611, 1655, 1822, 2626, 2857, 2935
BstMAI GTCTC 3 cut(s) 1469, 1518, 2797
BstMBI GATC 6 cut(s) 1608, 1652, 1819, 2623, 2854, 2932
BstMWI GCNNNNNNNGC 5 cut(s) 80, 654, 768, 923, 1008
BstNI CCWGG 3 cut(s) 192, 1082, 2042
BstNSI RCATGY 4 cut(s) 71, 771, 2089, 2180
BstPAI GACNNNNGTC 1 cut(s) 2961
BstSCI CCNGG 3 cut(s) 190, 1080, 2040
BstSFI CTRYAG 4 cut(s) 1009, 1446, 2540, 2730
BstUI CGCG 1 cut(s) 471
BstV1I GCAGC 5 cut(s) 344, 1526, 2260, 2526, 2611
BstV2I GAAGAC 1 cut(s) 2961
BstXI CCANNNNNNTGG 1 cut(s) 2048
BstZ17I GTATAC 2 cut(s) 1431, 2994
BsuI GTATCC 2 cut(s) 708, 1793
BsuRI GGCC 6 cut(s) 5, 298, 1149, 1242, 1834, 1861
BtgI CCRYGG 1 cut(s) 2687
BtgZI GCGATG 1 cut(s) 706
BtrI CACGTC 1 cut(s) 2054
BtsI GCAGTG 2 cut(s) 866, 978
BveI ACCTGC 1 cut(s) 2593
Cac8I GCNNGC 6 cut(s) 483, 769, 773, 1689, 2178, 2448
CaiI CAGNNNCTG 1 cut(s) 1041
CciI TCATGA 1 cut(s) 2553
CfoI GCGC 2 cut(s) 777, 1887
Cfr13I GGNCC 4 cut(s) 1147, 1241, 1955, 2009
CseI GACGC 1 cut(s) 524
Csp6I GTAC 6 cut(s) 518, 818, 853, 1113, 1549, 2900
CspCI CAANNNNNGTGG 2 cut(s) 2233, 2268
CviQI GTAC 6 cut(s) 518, 818, 853, 1113, 1549, 2900
DpnI GATC 6 cut(s) 1610, 1654, 1821, 2625, 2856, 2934
DpnII GATC 6 cut(s) 1608, 1652, 1819, 2623, 2854, 2932
EaeI YGGCCR 2 cut(s) 3, 1859
Eam1104I CTCTTC 6 cut(s) 17, 194, 287, 1374, 2188, 2403
EarI CTCTTC 6 cut(s) 17, 194, 287, 1374, 2188, 2403
EciI GGCGGA 1 cut(s) 1784
Ecl136II GAGCTC 1 cut(s) 955
Eco147I AGGCCT 1 cut(s) 298
Eco24I GRGCYC 1 cut(s) 957
Eco47I GGWCC 2 cut(s) 1955, 2009
Eco53kI GAGCTC 1 cut(s) 955
Eco57I CTGAAG 6 cut(s) 174, 513, 1968, 2142, 2251, 2942
Eco88I CYCGRG 1 cut(s) 1521
EcoICRI GAGCTC 1 cut(s) 955
EcoO109I RGGNCCY 1 cut(s) 1147
EcoRI GAATTC 1 cut(s) 2570
EcoRII CCWGG 3 cut(s) 190, 1080, 2040
EcoT22I ATGCAT 2 cut(s) 1358, 2771
EcoT38I GRGCYC 1 cut(s) 957
FalI AAGNNNNNCTT 4 cut(s) 1426, 1458, 2115, 2147
FaqI GGGAC 2 cut(s) 2022, 2522
FauI CCCGC 1 cut(s) 383
FauNDI CATATG 1 cut(s) 1057
FbaI TGATCA 3 cut(s) 1819, 2623, 2932
FblI GTMKAC 4 cut(s) 1430, 1921, 2521, 2993
Fnu4HI GCNGC 5 cut(s) 333, 1515, 2274, 2540, 2600
FriOI GRGCYC 1 cut(s) 957
Fsp4HI GCNGC 5 cut(s) 333, 1515, 2274, 2540, 2600
FspBI CTAG 3 cut(s) 1424, 2339, 2961
GlaI GCGC 2 cut(s) 776, 1886
GluI GCNGC 5 cut(s) 333, 1515, 2274, 2540, 2600
GsaI CCCAGC 2 cut(s) 87, 729
GsuI CTGGAG 4 cut(s) 941, 1103, 1151, 1584
HaeII RGCGCY 1 cut(s) 1888
HaeIII GGCC 6 cut(s) 5, 298, 1149, 1242, 1834, 1861
HapII CCGG 2 cut(s) 336, 2138
HgaI GACGC 1 cut(s) 524
HhaI GCGC 2 cut(s) 777, 1887
Hin1I GRCGYC 1 cut(s) 535
Hin6I GCGC 2 cut(s) 775, 1885
HinP1I GCGC 2 cut(s) 775, 1885
HindIII AAGCTT 2 cut(s) 2072, 2660
HpaII CCGG 2 cut(s) 336, 2138
HphI GGTGA 7 cut(s) 125, 452, 470, 1628, 2110, 2237, 2267
Hpy166II GTNNAC 8 cut(s) 1431, 1477, 1724, 1922, 2522, 2691, 2966, 2994
Hpy8I GTNNAC 8 cut(s) 1431, 1477, 1724, 1922, 2522, 2691, 2966, 2994
HpyCH4III ACNGT 6 cut(s) 122, 1107, 1553, 2588, 2644, 2688
HpyCH4IV ACGT 5 cut(s) 520, 707, 1502, 1734, 2053
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 654, 768, 923, 1008
HpySE526I ACGT 5 cut(s) 520, 707, 1502, 1734, 2053
Hsp92I GRCGYC 1 cut(s) 535
HspAI GCGC 2 cut(s) 775, 1885
Kpn2I TCCGGA 1 cut(s) 2137
Ksp22I TGATCA 3 cut(s) 1819, 2623, 2932
Kzo9I GATC 6 cut(s) 1608, 1652, 1819, 2623, 2854, 2932
LmnI GCTCC 9 cut(s) 544, 568, 930, 960, 1222, 1882, 2044, 2140, 2647
Lsp1109I GCAGC 5 cut(s) 344, 1526, 2260, 2526, 2611
MaeI CTAG 3 cut(s) 1424, 2339, 2961
MaeII ACGT 5 cut(s) 520, 707, 1502, 1734, 2053
MaeIII GTNAC 8 cut(s) 458, 1123, 2065, 2081, 2240, 2418, 2485, 2908
MalI GATC 6 cut(s) 1610, 1654, 1821, 2625, 2856, 2934
MboI GATC 6 cut(s) 1608, 1652, 1819, 2623, 2854, 2932
MfeI CAATTG 1 cut(s) 2209
MhlI GDGCHC 3 cut(s) 846, 957, 2652
MlsI TGGCCA 2 cut(s) 5, 1861
MluNI TGGCCA 2 cut(s) 5, 1861
MlyI GAGTC 3 cut(s) 941, 1120, 2486
MmeI TCCRAC 2 cut(s) 298, 2344
Mox20I TGGCCA 2 cut(s) 5, 1861
Mph1103I ATGCAT 2 cut(s) 1358, 2771
MroI TCCGGA 1 cut(s) 2137
MroXI GAANNNNTTC 1 cut(s) 2561
MscI TGGCCA 2 cut(s) 5, 1861
MseI TTAA 8 cut(s) 792, 857, 1572, 1661, 1929, 2295, 2664, 2975
MslI CAYNNNNRTG 1 cut(s) 2123
Msp20I TGGCCA 2 cut(s) 5, 1861
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 2 cut(s) 336, 2138
MspR9I CCNGG 3 cut(s) 192, 1082, 2042
MunI CAATTG 1 cut(s) 2209
Mva1269I GAATGC 1 cut(s) 1219
MvaI CCWGG 3 cut(s) 192, 1082, 2042
MvnI CGCG 1 cut(s) 471
MwoI GCNNNNNNNGC 5 cut(s) 80, 654, 768, 923, 1008
NdeI CATATG 1 cut(s) 1057
NdeII GATC 6 cut(s) 1608, 1652, 1819, 2623, 2854, 2932
NlaIV GGNNCC 4 cut(s) 1224, 1243, 1671, 2010
NmuCI GTSAC 6 cut(s) 458, 1123, 2081, 2240, 2485, 2908
NsiI ATGCAT 2 cut(s) 1358, 2771
NspI RCATGY 4 cut(s) 71, 771, 2089, 2180
NspV TTCGAA 1 cut(s) 2385
OliI CACNNNNGTG 1 cut(s) 2123
PaeI GCATGC 2 cut(s) 771, 2180
PaeR7I CTCGAG 1 cut(s) 1521
PagI TCATGA 1 cut(s) 2553
PceI AGGCCT 1 cut(s) 298
PciI ACATGT 2 cut(s) 67, 2085
PctI GAATGC 1 cut(s) 1219
PdmI GAANNNNTTC 1 cut(s) 2561
PfeI GAWTC 9 cut(s) 1087, 1293, 2060, 2128, 2258, 2382, 2387, 2393, 2679
PflFI GACNNNGTC 2 cut(s) 1481, 2789
PkrI GCNGC 5 cut(s) 334, 1516, 2275, 2541, 2601
PleI GAGTC 3 cut(s) 941, 1120, 2485
PpsI GAGTC 3 cut(s) 941, 1120, 2485
PscI ACATGT 2 cut(s) 67, 2085
PshAI GACNNNNGTC 1 cut(s) 2961
Psp124BI GAGCTC 1 cut(s) 957
Psp6I CCWGG 3 cut(s) 190, 1080, 2040
PspFI CCCAGC 2 cut(s) 83, 725
PspGI CCWGG 3 cut(s) 190, 1080, 2040
PspN4I GGNNCC 4 cut(s) 1224, 1243, 1671, 2010
PspPI GGNCC 4 cut(s) 1147, 1241, 1955, 2009
PstI CTGCAG 4 cut(s) 1013, 1450, 2544, 2734
PstNI CAGNNNCTG 1 cut(s) 1041
PsyI GACNNNGTC 2 cut(s) 1481, 2789
RsaI GTAC 6 cut(s) 519, 819, 854, 1114, 1550, 2901
RsaNI GTAC 6 cut(s) 518, 818, 853, 1113, 1549, 2900
RseI CAYNNNNRTG 1 cut(s) 2123
SacI GAGCTC 1 cut(s) 957
SaqAI TTAA 8 cut(s) 792, 857, 1572, 1661, 1929, 2295, 2664, 2975
SatI GCNGC 5 cut(s) 333, 1515, 2274, 2540, 2600
Sau3AI GATC 6 cut(s) 1608, 1652, 1819, 2623, 2854, 2932
Sau96I GGNCC 4 cut(s) 1147, 1241, 1955, 2009
SbfI CCTGCAGG 1 cut(s) 2734
ScaI AGTACT 2 cut(s) 819, 854
SchI GAGTC 3 cut(s) 941, 1120, 2486
ScrFI CCNGG 3 cut(s) 192, 1082, 2042
SdaI CCTGCAGG 1 cut(s) 2734
SduI GDGCHC 3 cut(s) 846, 957, 2652
SfcI CTRYAG 4 cut(s) 1009, 1446, 2540, 2730
Sfr274I CTCGAG 1 cut(s) 1521
SfuI TTCGAA 1 cut(s) 2385
SinI GGWCC 2 cut(s) 1955, 2009
SlaI CTCGAG 1 cut(s) 1521
SmiMI CAYNNNNRTG 1 cut(s) 2123
SmlI CTYRAG 2 cut(s) 1521, 2358
SmoI CTYRAG 2 cut(s) 1521, 2358
SpeI ACTAGT 3 cut(s) 1423, 2338, 2960
SphI GCATGC 2 cut(s) 771, 2180
Sse8387I CCTGCAGG 1 cut(s) 2734
SseBI AGGCCT 1 cut(s) 298
SsiI CCGC 7 cut(s) 390, 471, 563, 1769, 2096, 2741, 2839
SspI AATATT 3 cut(s) 1266, 2104, 2233
SspMI CTAG 3 cut(s) 1424, 2339, 2961
SstI GAGCTC 1 cut(s) 957
StuI AGGCCT 1 cut(s) 298
StyD4I CCNGG 3 cut(s) 190, 1080, 2040
TaaI ACNGT 6 cut(s) 122, 1107, 1553, 2588, 2644, 2688
TaiI ACGT 5 cut(s) 523, 710, 1505, 1737, 2056
TaqI TCGA 6 cut(s) 643, 1090, 1377, 1522, 2385, 2682
TaqII GACCGA 1 cut(s) 2205
TatI WGTACW 4 cut(s) 817, 852, 1548, 2899
TfiI GAWTC 9 cut(s) 1087, 1293, 2060, 2128, 2258, 2382, 2387, 2393, 2679
Tru1I TTAA 8 cut(s) 792, 857, 1572, 1661, 1929, 2295, 2664, 2975
Tru9I TTAA 8 cut(s) 792, 857, 1572, 1661, 1929, 2295, 2664, 2975
TseFI GTSAC 6 cut(s) 458, 1123, 2081, 2240, 2485, 2908
TseI GCWGC 5 cut(s) 332, 1514, 2273, 2539, 2599
Tsp45I GTSAC 6 cut(s) 458, 1123, 2081, 2240, 2485, 2908
TspGWI ACGGA 4 cut(s) 319, 1130, 1206, 2507
Tth111I GACNNNGTC 2 cut(s) 1481, 2789
VpaK11BI GGWCC 2 cut(s) 1955, 2009
XapI RAATTY 4 cut(s) 796, 2199, 2570, 2755
XceI RCATGY 4 cut(s) 71, 771, 2089, 2180
XcmI CCANNNNNNNNNTGG 1 cut(s) 1680
XhoI CTCGAG 1 cut(s) 1521
XmiI GTMKAC 4 cut(s) 1430, 1921, 2521, 2993
XmnI GAANNNNTTC 1 cut(s) 2561
XspI CTAG 3 cut(s) 1424, 2339, 2961
ZrmI AGTACT 2 cut(s) 819, 854
Zsp2I ATGCAT 2 cut(s) 1358, 2771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.