Rroxscaffold_4G00308520

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000280
Physical Location & Seq
Forward (+)
30071925 .. 30079693
7769 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00308520.1

Sequence Viewer

Length: 2805 bp
ATGAAGAGAGACGGCGTAAAGAAAGCTTATGATGACCCAGAACCCACATTTCCAGAGAAAGACGAGGGCTTTTTAGAAAACCAGAGTCACATGGCTGAGATTGTGAAGAAGAAGCGTAGTAGTAGGAAGAGAGCTACAGGGAAGGGATTGGCAGCCGTGAGTGACCCAATGGATTGTGAGAAAGGAGTTCTATCATGTGCTGCCAACTGGGGTCGGAAGCGGTTTAGGCGCGTGAGACCACGCAATGCTGCAAAGTGGATTGAGGATTGTTTAGAGTGCCTTCACTGTCAGGGGAATGATGAGGGCAGAGTTGTTTGCTGTGGAAGTTGCAAAAGAAAATGGTATCCTCAGATGTTAGAGGATGCCATTGCTGAGGCTTGTCCTGGATGCTGTGGTAATTGCAGTTGCAAGGCATGTTTGTGTGCAGCTTCTAGAAATTTGAAGAAACAGCCGGAGTTGAATATTAGCAAAGAGGAAAAACTTGAGCACTCTAAGTATTTGCTTCAAGCATTGCTTCCACTATTAAAACAACTTAACGATGAGCAAGTGATTGAGATGGAGATGGAGGCTAGGAGACAAGGGCTATCTGTTTCGGAGCTAAAGATCCAAAGGTCAAACTGCTCTTATGATGAGCGTGTGAATTGTAACAACTGCAAAGCTTCAATTGTTGATTTGTATAGAAGCTGCAATTTATGCTCATATGATCTCTGCCTAACGTGTTGCCGGGAGAGTCGAGATCGACACCTGGACGGAGTTGGGAAGGAAGTGACTATGGTGTATATCAATCAGGACCTAGAACATTTGCATGGTGGAAAGGGGAACATAGCGGAACTACTGTTTGAACCTAGCCCCAAGTGTCATGCAAGGTTAAAATCTGAATGGAAATCCAATGAAGATGGTAGCATCCTTTGCCCTCCAGAGGACAGGGATGGCTGTGGTCATGGTCATCTGGAATTGAGATGCATGTCTTTTGAGAATAAGGTCGGTGAGTTGGTACAGAAAGCAGAAGAGATGGCTGAAATTTACAAACTTATGCATGCGGCAGAAACTTCTGCACAGTGGTGTTCATGTTTCAATTCTTTGGATGTTTTTGATTCAATCAACAACACTAAAAAGTTAAGGAAAGGAGATTCTCGAAAGGATTTTGATGACAACTATTTGTACTGCCCAAGAGCTGGAAATATCCAACATGAGGATCTTAAGCATTTCCAATGGCATTGGCTCAGGGGTGAGCCTGTGATTGTTAGCAATGTAATTGAAACTACATCAGGTATGTGGCCTGCTTGTCGGCAGATGCAACAGACTATGCATGGTAAATATTTAACAATCAAGGCCATTGATTGCCTGGATTGGTGCAAGCAAGATATCAGTATCCATGAATTTTTTACTGGATATGCAAAGGGGTGGTTTGATTGGAGAATGGGGCCTCAAAGGCTAAAACTGATAGATTGCGCTCCAGCTAGTTTGATTGAGGAGCACCTTCCTTATCATGGTGCTGAGTTTATCTATGGCTTGCCATTTAAGGAATATACTCATCCTCATAGTGGGTTTCTTAATCTTGCTGCTAAATTCCCTGAGGAGTGTGGGAAGCCTGCTTATGGCATTGGGCAGGAGCTTGGACTTGAAGATTCTGTGACTAAGCTTCATTGCAATATATCTGATGCGGCCTATGATCCTGGTGAATATGGGAGGCTGGCATATTACGCTGCTGTAGTGAATGTTTTGACACATACTGCTGAAGTGACCCCTAATTCTCAACAACTTCTTACAGGAGAAAGGCTGAAAAAAGCGCAGATTGAGCAAGATAGAAGAGAAACTTTAGGAAACTGTCACTCTGCGGATGGCAATGTTGATGGTAAAATGCCTAGCGTTGGGTCATGTCTTAAACAATTTTATATTCAAGGTGGCAATCAAAACAGGGATGCTGCTTTCCAAAAACTAACACTACCGACTGTTCAATCTGACCGTGATACTGGTAATTCTTGTTTGAAAGGTGGAAATCTTTCAAACTATTCCGGATCTGAAAATAATGTAGATGGTGGTGCTTTGTGGGACATTTTTCAGAGACAGGACGTTCCAAAACTACAGGAATATCTCAGGAAGCACTTCAACGAGTTCAGGCACACATACTGCTGTCCGTCCCAACAGATTATTCATCCCATTCACGATCAGAATTTTTACCTGACTCGGGAGCACAAAAGGAAGCTGAAGGAGGAATGTGGAATCGAACCATGGACATGTATCCAGAAACTTGGGGATGCTATCTTTGTTCCTGCTGGCTGTCCATATCAAGTCAGAAACTTGAAGTCATGCATTAAAGTTGGAGTTCAATTTGTCTCACCTGAAAGTGTTGGCGACTGCCTTCGTTTGACTGGAGAATGCCGCACACTTCCAAAAGATCACAGGGCTAAAGAGGATAAATTGGAGGTGAAAAAAATGATTGTGCATGCTATGCGGGAAGCTGTGGATATTTTAGATCAGAGTGTGAGACAAGCAAAGAGAGAGGAAGTTGGACAAAGACAGCAAGTACAGAGCAGGGAGGATGGGGAAGTTCAAGCTGATGAGGAGCAGCCATCAATGTCATCACATCCAGCACAGTTCCCTCTTGATCTTGTTACAGCAATGCAGCGGCTAGATGACCTCGTGGAGCGCTTTTCGGATTTTGAACACTTGGAAGAGAGAGATAGAATAATGAATCCGGAATACCAGTTCTTATGTTCAGAGTTCATGAATTTATCTTCACATCTTGGTTATGCAACTCGGGTGAAAGAGTCTGCCATTCCAACATCCTATCAGCAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

934

Amino Acids

106.34

Weight (kDa)

6.23

Isoelectric Point (pI)

53.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjC PF02373 680 - 778 2.4e-12 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1175
AccII CGCG 1 cut(s) 231
AccIII TCCGGA 2 cut(s) 2015, 2698
AciI CCGC 8 cut(s) 220, 827, 1040, 1664, 1838, 2383, 2455, 2629
AclWI GGATC 4 cut(s) 598, 1203, 1667, 2026
AcsI RAATTY 6 cut(s) 436, 1020, 1379, 1568, 2173, 2731
AcuI CTGAAG 2 cut(s) 1758, 2228
AfaI GTAC 3 cut(s) 996, 1163, 2529
AfeI AGCGCT 1 cut(s) 2651
AfiI CCNNNNNNNGG 8 cut(s) 919, 1175, 1192, 1490, 1544, 1598, 1871, 2188
AflII CTTAAG 1 cut(s) 1199
AflIII ACRYGT 2 cut(s) 716, 2237
AjnI CCWGG 4 cut(s) 382, 744, 1344, 1675
AjuI GAANNNNNNNTTGG 2 cut(s) 2136, 2168
AleI CACNNNNGTG 1 cut(s) 1060
AloI GAACNNNNNNTCC 2 cut(s) 2693, 2725
Alw21I GWGCWC 3 cut(s) 489, 1479, 2196
Alw26I GTCTC 6 cut(s) 3, 229, 568, 2059, 2341, 2482
AlwI GGATC 4 cut(s) 598, 1203, 1667, 2026
Ama87I CYCGRG 2 cut(s) 2187, 2760
Aor13HI TCCGGA 2 cut(s) 2015, 2698
Aor51HI AGCGCT 1 cut(s) 2651
AoxI GGCC 4 cut(s) 1277, 1332, 1424, 1665
ApoI RAATTY 6 cut(s) 436, 1020, 1379, 1568, 2173, 2731
Asp700I GAANNNNTTC 2 cut(s) 2002, 2105
AspLEI GCGC 4 cut(s) 231, 1454, 1792, 2652
AspS9I GGNCC 2 cut(s) 790, 1424
AsuC2I CCSGG 1 cut(s) 725
AsuHPI GGTGA 6 cut(s) 998, 1241, 1691, 2331, 2440, 2776
AvaI CYCGRG 2 cut(s) 2187, 2760
AvaII GGWCC 1 cut(s) 790
AxyI CCTNAGG 1 cut(s) 1575
BauI CACGAG 1 cut(s) 2642
Bbv12I GWGCWC 3 cut(s) 489, 1479, 2196
BbvCI CCTCAGC 1 cut(s) 372
BceAI ACGGC 2 cut(s) 28, 140
BciT130I CCWGG 4 cut(s) 384, 746, 1346, 1677
BciVI GTATCC 3 cut(s) 354, 1382, 2252
BcnI CCSGG 1 cut(s) 725
BcoDI GTCTC 6 cut(s) 3, 229, 568, 2059, 2341, 2482
BfaI CTAG 7 cut(s) 432, 570, 794, 846, 1461, 1866, 2633
BfmI CTRYAG 3 cut(s) 135, 1710, 2084
BfoI RGCGCY 1 cut(s) 2653
BfrI CTTAAG 1 cut(s) 1199
BfuI GTATCC 3 cut(s) 354, 1382, 2252
BglI GCCNNNNNGGC 1 cut(s) 1432
Bme1390I CCNGG 5 cut(s) 384, 725, 746, 1346, 1677
Bme18I GGWCC 1 cut(s) 790
BmeT110I CYCGRG 2 cut(s) 2187, 2760
BmgT120I GGNCC 2 cut(s) 790, 1424
BmiI GGNNCC 1 cut(s) 1425
BmrFI CCNGG 5 cut(s) 384, 725, 746, 1346, 1677
BmrI ACTGGG 1 cut(s) 217
BmsI GCATC 8 cut(s) 352, 377, 912, 950, 1284, 1651, 1912, 2248
BmuI ACTGGG 1 cut(s) 217
BpmI CTGGAG 3 cut(s) 900, 1440, 2394
Bpu10I CCTNAGC 2 cut(s) 372, 1223
BpuEI CTTGAG 1 cut(s) 503
BpuMI CCSGG 1 cut(s) 725
BsaI GGTCTC 1 cut(s) 229
BsaJI CCNNGG 1 cut(s) 2231
BsaWI WCCGGW 2 cut(s) 2015, 2698
Bsc4I CCNNNNNNNGG 8 cut(s) 919, 1175, 1192, 1490, 1544, 1598, 1871, 2188
Bse1I ACTGG 5 cut(s) 212, 1393, 1978, 2377, 2707
Bse21I CCTNAGG 1 cut(s) 1575
Bse3DI GCAATG 7 cut(s) 250, 366, 509, 1255, 1645, 1852, 2628
BseAI TCCGGA 2 cut(s) 2015, 2698
BseBI CCWGG 4 cut(s) 384, 746, 1346, 1677
BseDI CCNNGG 1 cut(s) 2231
BseLI CCNNNNNNNGG 8 cut(s) 919, 1175, 1192, 1490, 1544, 1598, 1871, 2188
BseMI GCAATG 7 cut(s) 250, 366, 509, 1255, 1645, 1852, 2628
BseMII CTCAG 7 cut(s) 87, 362, 363, 1237, 1488, 1566, 2110
BseNI ACTGG 5 cut(s) 212, 1393, 1978, 2377, 2707
BseRI GAGGAG 3 cut(s) 1487, 1592, 2579
BsgI GTGCAG 2 cut(s) 444, 1038
Bsh1236I CGCG 1 cut(s) 231
BshFI GGCC 4 cut(s) 1279, 1334, 1426, 1667
BsiHKAI GWGCWC 3 cut(s) 489, 1479, 2196
BsiHKCI CYCGRG 2 cut(s) 2187, 2760
BsiSI CCGG 4 cut(s) 452, 724, 2016, 2699
BslFI GGGAC 2 cut(s) 2066, 2125
BslI CCNNNNNNNGG 8 cut(s) 919, 1175, 1192, 1490, 1544, 1598, 1871, 2188
BsmAI GTCTC 6 cut(s) 3, 229, 568, 2059, 2341, 2482
BsmBI CGTCTC 1 cut(s) 3
BsmFI GGGAC 2 cut(s) 2066, 2125
BsmI GAATGC 1 cut(s) 2384
BsnI GGCC 4 cut(s) 1279, 1334, 1426, 1667
Bso31I GGTCTC 1 cut(s) 229
BsoBI CYCGRG 2 cut(s) 2187, 2760
Bsp1286I GDGCHC 3 cut(s) 489, 1479, 2196
Bsp13I TCCGGA 2 cut(s) 2015, 2698
Bsp19I CCATGG 1 cut(s) 2231
BspACI CCGC 8 cut(s) 220, 827, 1040, 1664, 1838, 2383, 2455, 2629
BspANI GGCC 4 cut(s) 1279, 1334, 1426, 1667
BspCNI CTCAG 7 cut(s) 88, 361, 364, 1236, 1489, 1567, 2109
BspEI TCCGGA 2 cut(s) 2015, 2698
BspFNI CGCG 1 cut(s) 231
BspHI TCATGA 1 cut(s) 2727
BspLI GGNNCC 1 cut(s) 1425
BspPI GGATC 4 cut(s) 598, 1203, 1667, 2026
BspTI CTTAAG 1 cut(s) 1199
BspTNI GGTCTC 1 cut(s) 229
BsrDI GCAATG 7 cut(s) 250, 366, 509, 1255, 1645, 1852, 2628
BsrI ACTGG 5 cut(s) 212, 1393, 1978, 2377, 2707
BssECI CCNNGG 1 cut(s) 2231
BssSI CACGAG 1 cut(s) 2642
BssT1I CCWWGG 1 cut(s) 2231
Bst2BI CACGAG 1 cut(s) 2642
Bst2UI CCWGG 4 cut(s) 384, 746, 1346, 1677
Bst4CI ACNGT 8 cut(s) 287, 837, 1059, 1829, 1954, 1967, 2598, 2802
Bst6I CTCTTC 4 cut(s) 122, 1002, 1804, 2670
BstAFI CTTAAG 1 cut(s) 1199
BstAPI GCANNNNNTGC 3 cut(s) 693, 909, 2452
BstC8I GCNNGC 8 cut(s) 1038, 1281, 1358, 1514, 1593, 1695, 2278, 2448
BstDEI CTNAG 9 cut(s) 96, 348, 372, 492, 1223, 1497, 1575, 1638, 2096
BstDSI CCRYGG 1 cut(s) 2231
BstFNI CGCG 1 cut(s) 231
BstH2I RGCGCY 1 cut(s) 2653
BstHHI GCGC 4 cut(s) 231, 1454, 1792, 2652
BstMAI GTCTC 6 cut(s) 3, 229, 568, 2059, 2341, 2482
BstMWI GCNNNNNNNGC 7 cut(s) 226, 693, 909, 1432, 1703, 1798, 2452
BstNI CCWGG 4 cut(s) 384, 746, 1346, 1677
BstNSI RCATGY 5 cut(s) 417, 967, 1040, 2241, 2450
BstSCI CCNGG 5 cut(s) 382, 723, 744, 1344, 1675
BstSFI CTRYAG 3 cut(s) 135, 1710, 2084
BstUI CGCG 1 cut(s) 231
BstX2I RGATCY 3 cut(s) 603, 1195, 2018
BstXI CCANNNNNNTGG 1 cut(s) 2252
BstYI RGATCY 3 cut(s) 603, 1195, 2018
Bsu36I CCTNAGG 1 cut(s) 1575
BsuI GTATCC 3 cut(s) 354, 1382, 2252
BsuRI GGCC 4 cut(s) 1279, 1334, 1426, 1667
BtgI CCRYGG 1 cut(s) 2231
BtsIMutI CAGTG 2 cut(s) 283, 1064
Cac8I GCNNGC 8 cut(s) 1038, 1281, 1358, 1514, 1593, 1695, 2278, 2448
CciI TCATGA 1 cut(s) 2727
CfoI GCGC 4 cut(s) 231, 1454, 1792, 2652
Cfr13I GGNCC 2 cut(s) 790, 1424
Csp6I GTAC 3 cut(s) 995, 1162, 2528
CviQI GTAC 3 cut(s) 995, 1162, 2528
DdeI CTNAG 9 cut(s) 96, 348, 372, 492, 1223, 1497, 1575, 1638, 2096
Eam1104I CTCTTC 4 cut(s) 122, 1002, 1804, 2670
EarI CTCTTC 4 cut(s) 122, 1002, 1804, 2670
Eco130I CCWWGG 1 cut(s) 2231
Eco31I GGTCTC 1 cut(s) 229
Eco32I GATATC 1 cut(s) 1366
Eco47I GGWCC 1 cut(s) 790
Eco47III AGCGCT 1 cut(s) 2651
Eco57I CTGAAG 2 cut(s) 1758, 2228
Eco81I CCTNAGG 1 cut(s) 1575
Eco88I CYCGRG 2 cut(s) 2187, 2760
EcoO109I RGGNCCY 2 cut(s) 790, 1424
EcoRII CCWGG 4 cut(s) 382, 744, 1344, 1675
EcoRV GATATC 1 cut(s) 1366
EcoT14I CCWWGG 1 cut(s) 2231
EcoT22I ATGCAT 4 cut(s) 965, 1038, 1311, 2315
ErhI CCWWGG 1 cut(s) 2231
Esp3I CGTCTC 1 cut(s) 3
FalI AAGNNNNNCTT 4 cut(s) 498, 530, 1801, 1833
FaqI GGGAC 2 cut(s) 2066, 2125
FauI CCCGC 1 cut(s) 2448
FauNDI CATATG 1 cut(s) 700
FspBI CTAG 7 cut(s) 432, 570, 794, 846, 1461, 1866, 2633
GlaI GCGC 4 cut(s) 230, 1453, 1791, 2651
GsuI CTGGAG 3 cut(s) 900, 1440, 2394
HaeII RGCGCY 1 cut(s) 2653
HaeIII GGCC 4 cut(s) 1279, 1334, 1426, 1667
HapII CCGG 4 cut(s) 452, 724, 2016, 2699
HhaI GCGC 4 cut(s) 231, 1454, 1792, 2652
Hin6I GCGC 4 cut(s) 229, 1452, 1790, 2650
HinP1I GCGC 4 cut(s) 229, 1452, 1790, 2650
HindIII AAGCTT 3 cut(s) 24, 657, 1640
HinfI GANTC 9 cut(s) 85, 730, 1094, 1130, 1628, 2185, 2223, 2695, 2771
HpaII CCGG 4 cut(s) 452, 724, 2016, 2699
HphI GGTGA 6 cut(s) 998, 1241, 1691, 2331, 2440, 2776
HpyAV CCTTC 6 cut(s) 136, 290, 754, 1490, 2203, 2372
HpyCH4III ACNGT 8 cut(s) 287, 837, 1059, 1829, 1954, 1967, 2598, 2802
HpyCH4IV ACGT 2 cut(s) 716, 2073
HpyF10VI GCNNNNNNNGC 7 cut(s) 226, 693, 909, 1432, 1703, 1798, 2452
HpyF3I CTNAG 9 cut(s) 96, 348, 372, 492, 1223, 1497, 1575, 1638, 2096
HpySE526I ACGT 2 cut(s) 716, 2073
HspAI GCGC 4 cut(s) 229, 1452, 1790, 2650
Kpn2I TCCGGA 2 cut(s) 2015, 2698
LmnI GCTCC 7 cut(s) 595, 1459, 1474, 1612, 2191, 2566, 2647
LweI GCATC 8 cut(s) 352, 377, 912, 950, 1284, 1651, 1912, 2248
MaeI CTAG 7 cut(s) 432, 570, 794, 846, 1461, 1866, 2633
MaeII ACGT 2 cut(s) 716, 2073
MaeIII GTNAC 8 cut(s) 86, 161, 644, 766, 1633, 1741, 1829, 2614
MfeI CAATTG 1 cut(s) 663
MflI RGATCY 3 cut(s) 603, 1195, 2018
MhlI GDGCHC 3 cut(s) 489, 1479, 2196
MlyI GAGTC 4 cut(s) 94, 739, 2179, 2780
MmeI TCCRAC 4 cut(s) 194, 1210, 2302, 2491
Mph1103I ATGCAT 4 cut(s) 965, 1038, 1311, 2315
MroI TCCGGA 2 cut(s) 2015, 2698
MroXI GAANNNNTTC 2 cut(s) 2002, 2105
MslI CAYNNNNRTG 4 cut(s) 418, 804, 1060, 2236
MspA1I CMGCKG 1 cut(s) 2629
MspCI CTTAAG 1 cut(s) 1199
MspI CCGG 4 cut(s) 452, 724, 2016, 2699
MspR9I CCNGG 5 cut(s) 384, 725, 746, 1346, 1677
MunI CAATTG 1 cut(s) 663
Mva1269I GAATGC 1 cut(s) 2384
MvaI CCWGG 4 cut(s) 384, 746, 1346, 1677
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 7 cut(s) 226, 693, 909, 1432, 1703, 1798, 2452
NciI CCSGG 1 cut(s) 725
NcoI CCATGG 1 cut(s) 2231
NdeI CATATG 1 cut(s) 700
NlaIV GGNNCC 1 cut(s) 1425
NmuCI GTSAC 6 cut(s) 86, 161, 766, 1633, 1741, 1829
NsiI ATGCAT 4 cut(s) 965, 1038, 1311, 2315
NspI RCATGY 5 cut(s) 417, 967, 1040, 2241, 2450
OliI CACNNNNGTG 1 cut(s) 1060
PaeI GCATGC 2 cut(s) 1040, 2450
PagI TCATGA 1 cut(s) 2727
PciI ACATGT 1 cut(s) 2237
PctI GAATGC 1 cut(s) 2384
PdmI GAANNNNTTC 2 cut(s) 2002, 2105
PfeI GAWTC 5 cut(s) 1094, 1130, 1628, 2223, 2695
PflMI CCANNNNNTGG 1 cut(s) 1175
PfoI TCCNGGA 1 cut(s) 382
PleI GAGTC 4 cut(s) 93, 738, 2179, 2779
PpsI GAGTC 4 cut(s) 93, 738, 2179, 2779
PpuMI RGGWCCY 1 cut(s) 790
PscI ACATGT 1 cut(s) 2237
Psp5II RGGWCCY 1 cut(s) 790
Psp6I CCWGG 4 cut(s) 382, 744, 1344, 1675
PspGI CCWGG 4 cut(s) 382, 744, 1344, 1675
PspN4I GGNNCC 1 cut(s) 1425
PspPI GGNCC 2 cut(s) 790, 1424
PspPPI RGGWCCY 1 cut(s) 790
PsuI RGATCY 3 cut(s) 603, 1195, 2018
RsaI GTAC 3 cut(s) 996, 1163, 2529
RsaNI GTAC 3 cut(s) 995, 1162, 2528
RseI CAYNNNNRTG 4 cut(s) 418, 804, 1060, 2236
Sau96I GGNCC 2 cut(s) 790, 1424
SchI GAGTC 4 cut(s) 94, 739, 2179, 2780
ScrFI CCNGG 5 cut(s) 384, 725, 746, 1346, 1677
SduI GDGCHC 3 cut(s) 489, 1479, 2196
SfaNI GCATC 8 cut(s) 352, 377, 912, 950, 1284, 1651, 1912, 2248
SfcI CTRYAG 3 cut(s) 135, 1710, 2084
SinI GGWCC 1 cut(s) 790
SmiMI CAYNNNNRTG 4 cut(s) 418, 804, 1060, 2236
SmlI CTYRAG 2 cut(s) 482, 1199
SmoI CTYRAG 2 cut(s) 482, 1199
SphI GCATGC 2 cut(s) 1040, 2450
SsiI CCGC 8 cut(s) 220, 827, 1040, 1664, 1838, 2383, 2455, 2629
SspI AATATT 2 cut(s) 463, 1319
SspMI CTAG 7 cut(s) 432, 570, 794, 846, 1461, 1866, 2633
StyD4I CCNGG 5 cut(s) 382, 723, 744, 1344, 1675
StyI CCWWGG 1 cut(s) 2231
TaaI ACNGT 8 cut(s) 287, 837, 1059, 1829, 1954, 1967, 2598, 2802
TaiI ACGT 2 cut(s) 719, 2076
TaqI TCGA 4 cut(s) 733, 739, 1135, 2226
TatI WGTACW 2 cut(s) 1161, 2527
TauI GCSGC 4 cut(s) 1043, 1667, 2385, 2632
TfiI GAWTC 5 cut(s) 1094, 1130, 1628, 2223, 2695
TscAI CASTG 2 cut(s) 290, 1064
TseFI GTSAC 6 cut(s) 86, 161, 766, 1633, 1741, 1829
Tsp45I GTSAC 6 cut(s) 86, 161, 766, 1633, 1741, 1829
TspDTI ATGAA 9 cut(s) 17, 906, 1056, 1392, 1634, 2144, 2708, 2716, 2744
TspGWI ACGGA 2 cut(s) 765, 2127
TspRI CASTG 2 cut(s) 290, 1064
Van91I CCANNNNNTGG 1 cut(s) 1175
Vha464I CTTAAG 1 cut(s) 1199
VpaK11BI GGWCC 1 cut(s) 790
XapI RAATTY 6 cut(s) 436, 1020, 1379, 1568, 2173, 2731
XbaI TCTAGA 1 cut(s) 431
XceI RCATGY 5 cut(s) 417, 967, 1040, 2241, 2450
XcmI CCANNNNNNNNNTGG 1 cut(s) 1342
XmnI GAANNNNTTC 2 cut(s) 2002, 2105
XspI CTAG 7 cut(s) 432, 570, 794, 846, 1461, 1866, 2633
Zsp2I ATGCAT 4 cut(s) 965, 1038, 1311, 2315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.