Rmu_sc0004540.1_g000014

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004540.1
OG0000280
Physical Location & Seq
Forward (+)
77334 .. 80279
2946 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004540.1_g000014.1.cds

Sequence Viewer

Length: 2946 bp
atggcggcgagaaagaaaaccaaaggcagaggccggccgaggcaagaagagaacggcgctggtctggaggagatgaaggccgagggtattttgggaattgagaacgagggagctaatggggaagtaaaacaagaagctgtggctcctttggtggagggcagaggcaggaagcggcggaggaagaaacaagaagaagacggtggtggtggtgagggtgttttgggaaatcaaaacgaggaagaggttgctgaagatggtgtggagttaaagacgcgttatccacagagggcggcaagggctgtaagggcggtggtggaggaggaggaggagccggagattcggaagaggacgaagaagagaaaagcagtggtggtggagggggaggaggagccggagattcggaagatgatgaagaagagaaaagcagcggcggcggagggagaggaagatgaggcagtgaaggacgtttcaacaatgtgccatcagtgtcagaggaatgataaagggagagtggttcggtgcacgagttgtccgaaaaggggtaagggaatgaggaagaggtattgtatcccttgcatacagaggtggtatcccaacacatcggaggaagccattgctgaggcttgtcctgtgtgccgcggcaactgcaactgcaaagcctgccttcgactggatgtgcctaataggtgtttgaagaatctggacccgaaaatcaacgaggagaccaagctggagcactgtaagtatttggttcatcagctgcttccgtatttgaagaggattagggacgagcaggtgagtgagatggtggtggaagccaagaggcagggattaagtgatgtttcagagctcaaggtggagaaggctaattgcgctgacgatgagcgtatgtactgcaataactgcagcacttcgatttttgattttcttaggagttgtcctgagtgtgagtatgatctgtgcctcatttgctgccgggagattcgagatggcaagtggaagaagagaggtatggaggaggaagagagtcagaagtatgtcaatttggggcgtgagtatttgcatggaaagggagatttgaagagcttgctaaaaaaaaagagagaaaaggagttgaagagcttgctagagaagaaaagagaagaagagttgaaggagaatgttgatgtgggagtgaagctggactcgtcgtcagaatctgctcaagagtccacgagagcaagtgaagatggaaatattcaagatgggcagcagaaaaagagaggcagagaggatgaagtaagtgaggaatttgttaatgcgggggagtgtttggatgaagagggagagctgaagaggaatgatgatgtgggagtgaagcaggacgagtctgaatctgctgaagagtccacatttgagtggagagcaagtgaagatggaagcattatctgccccccaacacacatgaaaggttgtggtaagcataatttagaattgaggtgcaatttttctcagaatcatgtcatagacttggtagagaaagcaggaaaaatagatgaaagttacaatcctatgcatgctagtagtgaaacatgtgcacaaaagtgttcatgtgttaaacctgtgaatgatgctgtcaactcaagtagcagcagctgtaagttaagaaaagcagcttctagagacagttctgatgataactacttgttctgtccaagggttggagaaatccaagatgaggattttgggcattttcagtggcattggatgaggggtgagcctgtgatcgttagcaatgcacttcaaaatggatcaggtttgagctgggaaccattagtcatgtggcgtgcatttaggcagataaagaatctcaagcatgatagagaattggcagtccataccattgaatgcttggattggagtttcaaagaaactaataacctccatttctttactggatattccgaaggccggcctgatgagaaaggatggcctgagattctcaagctcaaagacttcccgccatctactgagtttgacaatcgtctcccccggcatggtaaggaatttgtctgttgtctgcctttcaaagagtatactcaccccaacaagggaaccctgaaccttgctatccacttgccacctacctcagtaaagccggacatgggtccaaaaacatatattgcctatggttttgcacaggagcttggacggggagattctgtgaccaagcttcactgcgatatgtctgatgcggttaatattctgacacatacagctgaagtggcccttacaactgaacaacttgcaactatagataagttgaaaaaaaagcacaggaagcaagacgaaagagagatatttgggaattgtcacacaagtgctggaaatggaagtgggcctcaggaagaatcagctgaagtatgtgaggatggtggtgccttatgggacattttccggatccaggatgttccgcagctggagaaatacatccacaagcacatcaatgaatttaggcatgtgcactgtaatcctttgcagcgtgttactcatgctatacatgatcagactgtttatctcactgtggagcacaagaggaagctcaaggaggaattcggtatcgagccgtggacatttgtccaaaaacttggagatgctgttttcattcctgcaggttgtcctcatcaggtgaggaacttgaagtcttgtatcaaagttgcactggactttgtttcccctgagaatgtgggtcagtgctttcatttgacggaagagtttcgcacacttccgccaaaccacagggccagcgaggacaagctggaagtgaagaaaatgattgtgcatgctgtttgtgaagctgtgaaggagatttctggtgagaatgcgaagcctgaggatgtgaagcctaagaggaggtgcaaggggcctaagaggaggaggcgcaaggggaacaaaggagaggtgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

981

Amino Acids

111.6

Weight (kDa)

6.63

Isoelectric Point (pI)

55.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 784
Acc36I ACCTGC 2 cut(s) 784, 2642
AccB1I GGYRCC 1 cut(s) 2417
AccB7I CCANNNNNTGG 1 cut(s) 1700
AccI GTMKAC 1 cut(s) 2075
AccII CGCG 2 cut(s) 274, 639
AccIII TCCGGA 1 cut(s) 2436
AclWI GGATC 3 cut(s) 1798, 2434, 2447
AcoI YGGCCR 1 cut(s) 35
AcsI RAATTY 4 cut(s) 1289, 2045, 2489, 2591
AcuI CTGAAG 5 cut(s) 270, 1352, 1401, 2280, 2418
AfaI GTAC 1 cut(s) 893
AflIII ACRYGT 2 cut(s) 272, 1571
AhdI GACNNNNNGTC 1 cut(s) 1189
AjnI CCWGG 1 cut(s) 2442
AleI CACNNNNGTG 2 cut(s) 1582, 2358
Alw21I GWGCWC 6 cut(s) 524, 738, 852, 1579, 2505, 2571
Alw26I GTCTC 3 cut(s) 716, 1656, 2030
Alw44I GTGCAC 3 cut(s) 520, 1575, 2501
AlwI GGATC 3 cut(s) 1798, 2434, 2447
AlwNI CAGNNNCTG 2 cut(s) 1199, 1635
Aor13HI TCCGGA 1 cut(s) 2436
ApaLI GTGCAC 3 cut(s) 520, 1575, 2501
ApoI RAATTY 4 cut(s) 1289, 2045, 2489, 2591
Asp700I GAANNNNTTC 1 cut(s) 2754
AspLEI GCGC 3 cut(s) 59, 875, 2922
AspS9I GGNCC 6 cut(s) 703, 2147, 2266, 2378, 2781, 2903
AsuC2I CCSGG 2 cut(s) 977, 2032
AsuHPI GGTGA 6 cut(s) 221, 808, 1766, 2072, 2680, 2867
AvaII GGWCC 2 cut(s) 703, 2147
AxyI CCTNAGG 2 cut(s) 2382, 2871
BaeGI GKGCMC 3 cut(s) 524, 1579, 2505
BamHI GGATCC 1 cut(s) 2439
BanI GGYRCC 1 cut(s) 2417
BanII GRGCYC 1 cut(s) 852
BauI CACGAG 2 cut(s) 523, 1213
BbsI GAAGAC 1 cut(s) 201
Bbv12I GWGCWC 6 cut(s) 524, 738, 852, 1579, 2505, 2571
BbvCI CCTCAGC 1 cut(s) 618
BceAI ACGGC 2 cut(s) 70, 2590
BciT130I CCWGG 1 cut(s) 2444
BciVI GTATCC 2 cut(s) 578, 600
BclI TGATCA 1 cut(s) 2542
BcnI CCSGG 2 cut(s) 977, 2032
BcoDI GTCTC 3 cut(s) 716, 1656, 2030
BfaI CTAG 3 cut(s) 1127, 1560, 1659
BfmI CTRYAG 3 cut(s) 904, 2292, 2649
BfoI RGCGCY 1 cut(s) 60
BfuAI ACCTGC 2 cut(s) 784, 2642
BfuI GTATCC 2 cut(s) 578, 600
Bme1390I CCNGG 3 cut(s) 977, 2032, 2444
Bme18I GGWCC 2 cut(s) 703, 2147
BmeRI GACNNNNNGTC 1 cut(s) 1189
BmgT120I GGNCC 6 cut(s) 703, 2147, 2266, 2378, 2781, 2903
BmrFI CCNGG 3 cut(s) 977, 2032, 2444
BmsI GCATC 3 cut(s) 1600, 2221, 2623
BoxI GACNNNNGTC 3 cut(s) 2022, 2145, 2615
BpiI GAAGAC 1 cut(s) 201
BpmI CTGGAG 3 cut(s) 86, 752, 2480
Bpu10I CCTNAGC 1 cut(s) 618
BpuEI CTTGAG 6 cut(s) 836, 1188, 1606, 1835, 1967, 2567
BpuMI CCSGG 2 cut(s) 977, 2032
BsaI GGTCTC 1 cut(s) 716
BsaJI CCNNGG 6 cut(s) 38, 81, 637, 1694, 2030, 2606
BsaWI WCCGGW 1 cut(s) 2436
BsaXI ACNNNNNCTCC 6 cut(s) 1393, 1423, 2187, 2217, 2622, 2652
Bse118I RCCGGY 2 cut(s) 33, 1950
Bse1I ACTGG 3 cut(s) 675, 1939, 2706
Bse21I CCTNAGG 2 cut(s) 2382, 2871
Bse3DI GCAATG 2 cut(s) 612, 1780
BseAI TCCGGA 1 cut(s) 2436
BseBI CCWGG 1 cut(s) 2444
BseDI CCNNGG 6 cut(s) 38, 81, 637, 1694, 2030, 2606
BseGI GGATG 9 cut(s) 679, 1279, 1321, 1752, 1973, 2416, 2452, 2469, 2881
BseMI GCAATG 2 cut(s) 612, 1780
BseMII CTCAG 9 cut(s) 609, 933, 1505, 1965, 2001, 2142, 2396, 2709, 2862
BseNI ACTGG 3 cut(s) 675, 1939, 2706
BseSI GKGCMC 3 cut(s) 524, 1579, 2505
BseX3I CGGCCG 1 cut(s) 35
BseYI CCCAGC 1 cut(s) 1803
Bsh1236I CGCG 2 cut(s) 274, 639
Bsh1285I CGRYCG 1 cut(s) 38
BshNI GGYRCC 1 cut(s) 2417
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 6 cut(s) 524, 738, 852, 1579, 2505, 2571
BsiSI CCGG 8 cut(s) 34, 332, 392, 976, 1951, 2032, 2138, 2437
BslFI GGGAC 2 cut(s) 800, 2441
BsmAI GTCTC 3 cut(s) 716, 1656, 2030
BsmBI CGTCTC 1 cut(s) 2030
BsmFI GGGAC 2 cut(s) 800, 2441
BsmI GAATGC 2 cut(s) 1892, 2866
Bso31I GGTCTC 1 cut(s) 716
Bsp1286I GDGCHC 6 cut(s) 524, 738, 852, 1579, 2505, 2571
Bsp13I TCCGGA 1 cut(s) 2436
Bsp143I GATC 5 cut(s) 955, 1764, 1790, 2439, 2542
BspCNI CTCAG 9 cut(s) 610, 934, 1504, 1966, 2002, 2141, 2395, 2710, 2863
BspEI TCCGGA 1 cut(s) 2436
BspFNI CGCG 2 cut(s) 274, 639
BspMAI CTGCAG 2 cut(s) 908, 2653
BspMI ACCTGC 2 cut(s) 784, 2642
BspPI GGATC 3 cut(s) 1798, 2434, 2447
BspQI GCTCTTC 2 cut(s) 1076, 1112
BspT107I GGYRCC 1 cut(s) 2417
BspTNI GGTCTC 1 cut(s) 716
BsrDI GCAATG 2 cut(s) 612, 1780
BsrFI RCCGGY 2 cut(s) 33, 1950
BsrI ACTGG 3 cut(s) 675, 1939, 2706
BssAI RCCGGY 2 cut(s) 33, 1950
BssECI CCNNGG 6 cut(s) 38, 81, 637, 1694, 2030, 2606
BssMI GATC 5 cut(s) 955, 1764, 1790, 2439, 2542
BssNAI GTATAC 1 cut(s) 2076
BssSI CACGAG 2 cut(s) 523, 1213
BssT1I CCWWGG 1 cut(s) 1694
Bst1107I GTATAC 1 cut(s) 2076
Bst2BI CACGAG 2 cut(s) 523, 1213
Bst2UI CCWGG 1 cut(s) 2444
Bst4CI ACNGT 6 cut(s) 200, 740, 1667, 2507, 2551, 2563
BstAPI GCANNNNNTGC 3 cut(s) 660, 903, 1428
BstC8I GCNNGC 9 cut(s) 35, 661, 1088, 1124, 1557, 1827, 1952, 2785, 2823
BstDSI CCRYGG 2 cut(s) 637, 2606
BstF5I GGATG 9 cut(s) 679, 1279, 1321, 1752, 1973, 2416, 2452, 2469, 2881
BstFNI CGCG 2 cut(s) 274, 639
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 3 cut(s) 59, 875, 2922
BstKTI GATC 5 cut(s) 958, 1767, 1793, 2442, 2545
BstMAI GTCTC 3 cut(s) 716, 1656, 2030
BstMBI GATC 5 cut(s) 955, 1764, 1790, 2439, 2542
BstMCI CGRYCG 1 cut(s) 38
BstNI CCWGG 1 cut(s) 2444
BstNSI RCATGY 4 cut(s) 1559, 1575, 2501, 2825
BstPAI GACNNNNGTC 3 cut(s) 2022, 2145, 2615
BstSCI CCNGG 3 cut(s) 975, 2030, 2442
BstSFI CTRYAG 3 cut(s) 904, 2292, 2649
BstSLI GKGCMC 3 cut(s) 524, 1579, 2505
BstUI CGCG 2 cut(s) 274, 639
BstV2I GAAGAC 1 cut(s) 201
BstX2I RGATCY 1 cut(s) 2439
BstXI CCANNNNNNTGG 1 cut(s) 2627
BstYI RGATCY 1 cut(s) 2439
BstZ17I GTATAC 1 cut(s) 2076
BstZI CGGCCG 1 cut(s) 35
Bsu36I CCTNAGG 2 cut(s) 2382, 2871
BsuI GTATCC 2 cut(s) 578, 600
BtgI CCRYGG 2 cut(s) 637, 2606
BtsCI GGATG 9 cut(s) 679, 1279, 1321, 1752, 1973, 2416, 2452, 2469, 2881
BtsI GCAGTG 3 cut(s) 372, 462, 2215
BveI ACCTGC 2 cut(s) 784, 2642
Cac8I GCNNGC 9 cut(s) 35, 661, 1088, 1124, 1557, 1827, 1952, 2785, 2823
CaiI CAGNNNCTG 2 cut(s) 1199, 1635
CfoI GCGC 3 cut(s) 59, 875, 2922
Cfr10I RCCGGY 2 cut(s) 33, 1950
Cfr13I GGNCC 6 cut(s) 703, 2147, 2266, 2378, 2781, 2903
Cfr42I CCGCGG 1 cut(s) 640
CseI GACGC 1 cut(s) 280
Csp6I GTAC 1 cut(s) 892
CviQI GTAC 1 cut(s) 892
DpnI GATC 5 cut(s) 957, 1766, 1792, 2441, 2544
DpnII GATC 5 cut(s) 955, 1764, 1790, 2439, 2542
DriI GACNNNNNGTC 1 cut(s) 1189
EaeI YGGCCR 1 cut(s) 35
EagI CGGCCG 1 cut(s) 35
Eam1105I GACNNNNNGTC 1 cut(s) 1189
EciI GGCGGA 3 cut(s) 190, 449, 2757
Ecl136II GAGCTC 1 cut(s) 850
EclXI CGGCCG 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 1694
Eco24I GRGCYC 1 cut(s) 852
Eco31I GGTCTC 1 cut(s) 716
Eco47I GGWCC 2 cut(s) 703, 2147
Eco52I CGGCCG 1 cut(s) 35
Eco53kI GAGCTC 1 cut(s) 850
Eco57I CTGAAG 5 cut(s) 270, 1352, 1401, 2280, 2418
Eco81I CCTNAGG 2 cut(s) 2382, 2871
EcoICRI GAGCTC 1 cut(s) 850
EcoO109I RGGNCCY 1 cut(s) 2903
EcoRI GAATTC 1 cut(s) 2591
EcoRII CCWGG 1 cut(s) 2442
EcoT14I CCWWGG 1 cut(s) 1694
EcoT22I ATGCAT 1 cut(s) 1557
EcoT38I GRGCYC 1 cut(s) 852
ErhI CCWWGG 1 cut(s) 1694
Esp3I CGTCTC 1 cut(s) 2030
FalI AAGNNNNNCTT 4 cut(s) 648, 680, 2253, 2285
FaqI GGGAC 2 cut(s) 800, 2441
FauI CCCGC 2 cut(s) 1294, 2007
FbaI TGATCA 1 cut(s) 2542
FblI GTMKAC 1 cut(s) 2075
FokI GGATG 9 cut(s) 686, 1286, 1328, 1759, 1980, 2423, 2456, 2459, 2888
FriOI GRGCYC 1 cut(s) 852
FseI GGCCGGCC 2 cut(s) 37, 1954
FspBI CTAG 3 cut(s) 1127, 1560, 1659
GlaI GCGC 3 cut(s) 58, 874, 2921
GsaI CCCAGC 1 cut(s) 1807
GsuI CTGGAG 3 cut(s) 86, 752, 2480
HaeII RGCGCY 1 cut(s) 60
HapII CCGG 8 cut(s) 34, 332, 392, 976, 1951, 2032, 2138, 2437
HgaI GACGC 1 cut(s) 280
HhaI GCGC 3 cut(s) 59, 875, 2922
Hin6I GCGC 3 cut(s) 57, 873, 2920
HinP1I GCGC 3 cut(s) 57, 873, 2920
HincII GTYRAC 1 cut(s) 1618
HindII GTYRAC 1 cut(s) 1618
HindIII AAGCTT 1 cut(s) 2210
HpaII CCGG 8 cut(s) 34, 332, 392, 976, 1951, 2032, 2138, 2437
HphI GGTGA 6 cut(s) 221, 808, 1766, 2072, 2680, 2867
Hpy166II GTNNAC 8 cut(s) 522, 1212, 1389, 1577, 1618, 2076, 2503, 2610
Hpy188III TCNNGA 9 cut(s) 65, 701, 941, 986, 1205, 1241, 1659, 2384, 2437
Hpy8I GTNNAC 8 cut(s) 522, 1212, 1389, 1577, 1618, 2076, 2503, 2610
Hpy99I CGWCG 1 cut(s) 1192
HpyAV CCTTC 7 cut(s) 70, 454, 674, 856, 1147, 1940, 2836
HpyCH4III ACNGT 6 cut(s) 200, 740, 1667, 2507, 2551, 2563
HpyCH4IV ACGT 1 cut(s) 465
HpySE526I ACGT 1 cut(s) 465
HspAI GCGC 3 cut(s) 57, 873, 2920
Kpn2I TCCGGA 1 cut(s) 2436
KroI GCCGGC 2 cut(s) 33, 1950
KroNI GCCGGC 2 cut(s) 35, 1952
Ksp22I TGATCA 1 cut(s) 2542
KspI CCGCGG 1 cut(s) 640
Kzo9I GATC 5 cut(s) 955, 1764, 1790, 2439, 2542
LguI GCTCTTC 2 cut(s) 1076, 1112
LmnI GCTCC 7 cut(s) 110, 148, 328, 388, 733, 2182, 2566
LweI GCATC 3 cut(s) 1600, 2221, 2623
MaeI CTAG 3 cut(s) 1127, 1560, 1659
MaeII ACGT 1 cut(s) 465
MaeIII GTNAC 4 cut(s) 1541, 2203, 2351, 2524
MalI GATC 5 cut(s) 957, 1766, 1792, 2441, 2544
MboI GATC 5 cut(s) 955, 1764, 1790, 2439, 2542
MflI RGATCY 1 cut(s) 2439
MhlI GDGCHC 6 cut(s) 524, 738, 852, 1579, 2505, 2571
MluI ACGCGT 1 cut(s) 272
MlyI GAGTC 5 cut(s) 1036, 1178, 1217, 1376, 1394
MmeI TCCRAC 1 cut(s) 1681
Mph1103I ATGCAT 1 cut(s) 1557
MroI TCCGGA 1 cut(s) 2436
MroNI GCCGGC 2 cut(s) 33, 1950
MroXI GAANNNNTTC 1 cut(s) 2754
MseI TTAA 6 cut(s) 266, 833, 1296, 1596, 1643, 2238
MslI CAYNNNNRTG 4 cut(s) 1396, 1582, 2358, 2484
MspA1I CMGCKG 7 cut(s) 428, 639, 760, 1635, 2258, 2396, 2458
MspI CCGG 8 cut(s) 34, 332, 392, 976, 1951, 2032, 2138, 2437
MspR9I CCNGG 3 cut(s) 977, 2032, 2444
Mva1269I GAATGC 2 cut(s) 1892, 2866
MvaI CCWGG 1 cut(s) 2444
MvnI CGCG 2 cut(s) 274, 639
NaeI GCCGGC 2 cut(s) 35, 1952
NciI CCSGG 2 cut(s) 977, 2032
NdeII GATC 5 cut(s) 955, 1764, 1790, 2439, 2542
NgoMIV GCCGGC 2 cut(s) 33, 1950
NmeAIII GCCGAG 2 cut(s) 63, 106
NmuCI GTSAC 2 cut(s) 2203, 2351
NsiI ATGCAT 1 cut(s) 1557
NspI RCATGY 4 cut(s) 1559, 1575, 2501, 2825
OliI CACNNNNGTG 2 cut(s) 1582, 2358
PaeI GCATGC 2 cut(s) 1559, 2825
PaqCI CACCTGC 1 cut(s) 784
PciI ACATGT 1 cut(s) 1571
PciSI GCTCTTC 2 cut(s) 1076, 1112
PcsI WCGNNNNNNNCGW 1 cut(s) 530
PctI GAATGC 2 cut(s) 1892, 2866
PdiI GCCGGC 2 cut(s) 35, 1952
PdmI GAANNNNTTC 1 cut(s) 2754
PflMI CCANNNNNTGG 1 cut(s) 1700
PfoI TCCNGGA 1 cut(s) 2442
PleI GAGTC 5 cut(s) 1035, 1178, 1216, 1375, 1393
PpsI GAGTC 5 cut(s) 1035, 1178, 1216, 1375, 1393
PscI ACATGT 1 cut(s) 1571
PshAI GACNNNNGTC 3 cut(s) 2022, 2145, 2615
Psp124BI GAGCTC 1 cut(s) 852
Psp6I CCWGG 1 cut(s) 2442
PspFI CCCAGC 1 cut(s) 1803
PspGI CCWGG 1 cut(s) 2442
PspPI GGNCC 6 cut(s) 703, 2147, 2266, 2378, 2781, 2903
PstI CTGCAG 2 cut(s) 908, 2653
PstNI CAGNNNCTG 2 cut(s) 1199, 1635
PsuI RGATCY 1 cut(s) 2439
PvuII CAGCTG 5 cut(s) 760, 1635, 2258, 2396, 2458
RigI GGCCGGCC 2 cut(s) 37, 1954
RsaI GTAC 1 cut(s) 893
RsaNI GTAC 1 cut(s) 892
RseI CAYNNNNRTG 4 cut(s) 1396, 1582, 2358, 2484
SacI GAGCTC 1 cut(s) 852
SacII CCGCGG 1 cut(s) 640
SapI GCTCTTC 2 cut(s) 1076, 1112
SaqAI TTAA 6 cut(s) 266, 833, 1296, 1596, 1643, 2238
Sau3AI GATC 5 cut(s) 955, 1764, 1790, 2439, 2542
Sau96I GGNCC 6 cut(s) 703, 2147, 2266, 2378, 2781, 2903
SbfI CCTGCAGG 1 cut(s) 2653
SchI GAGTC 5 cut(s) 1036, 1178, 1217, 1376, 1394
ScrFI CCNGG 3 cut(s) 977, 2032, 2444
SdaI CCTGCAGG 1 cut(s) 2653
SduI GDGCHC 6 cut(s) 524, 738, 852, 1579, 2505, 2571
SfaNI GCATC 3 cut(s) 1600, 2221, 2623
SfcI CTRYAG 3 cut(s) 904, 2292, 2649
Sfr303I CCGCGG 1 cut(s) 640
SgrBI CCGCGG 1 cut(s) 640
SinI GGWCC 2 cut(s) 703, 2147
SmiMI CAYNNNNRTG 4 cut(s) 1396, 1582, 2358, 2484
SmlI CTYRAG 6 cut(s) 851, 1203, 1621, 1850, 1982, 2582
SmoI CTYRAG 6 cut(s) 851, 1203, 1621, 1850, 1982, 2582
SphI GCATGC 2 cut(s) 1559, 2825
Sse8387I CCTGCAGG 1 cut(s) 2653
SspI AATATT 2 cut(s) 1237, 2242
SspMI CTAG 3 cut(s) 1127, 1560, 1659
SstI GAGCTC 1 cut(s) 852
StyD4I CCNGG 3 cut(s) 975, 2030, 2442
StyI CCWWGG 1 cut(s) 1694
TaaI ACNGT 6 cut(s) 200, 740, 1667, 2507, 2551, 2563
TaiI ACGT 1 cut(s) 468
TaqI TCGA 4 cut(s) 667, 914, 985, 2601
TatI WGTACW 1 cut(s) 891
TauI GCSGC 7 cut(s) 8, 175, 293, 431, 434, 639, 642
Tru1I TTAA 6 cut(s) 266, 833, 1296, 1596, 1643, 2238
Tru9I TTAA 6 cut(s) 266, 833, 1296, 1596, 1643, 2238
TseFI GTSAC 2 cut(s) 2203, 2351
Tsp45I GTSAC 2 cut(s) 2203, 2351
TspGWI ACGGA 2 cut(s) 756, 2762
Van91I CCANNNNNTGG 1 cut(s) 1700
VneI GTGCAC 3 cut(s) 520, 1575, 2501
VpaK11BI GGWCC 2 cut(s) 703, 2147
XapI RAATTY 4 cut(s) 1289, 2045, 2489, 2591
XbaI TCTAGA 1 cut(s) 1658
XceI RCATGY 4 cut(s) 1559, 1575, 2501, 2825
XcmI CCANNNNNNNNNTGG 3 cut(s) 1818, 1888, 1931
XmiI GTMKAC 1 cut(s) 2075
XmnI GAANNNNTTC 1 cut(s) 2754
XspI CTAG 3 cut(s) 1127, 1560, 1659
Zsp2I ATGCAT 1 cut(s) 1557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.