Rroxscaffold_3G00235180

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000280
Physical Location & Seq
Reverse (-)
22043273 .. 22050807
7535 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00235180.1

Sequence Viewer

Length: 5751 bp
ATGGAATTCCAGGCCACGGCGGCGAGATTGAGGCCCCGAGGCACAAAGAGGCCGAGCGGCGGCGGTGTGGTGGAGAAGGCGGCCGAGGGTATTTTGGGAATTCAGAACGGCGACGGAACAATTGGGGAAGACGTGGAGCCAGCAGCGCCAAAGCTTGTTCCCTGGGGGAGGAAGCGGCGGAGGACAGGATGCGAGGGTATTTTGGGAAATCAAACTGAAGGAGAGGCAAGGGCGGCAAATTGGTGCACAAGGGCTCCCTTGGATGTGGGGAAATGTTGCCATCAATGTAAGCGAAATTGTAAGCGAAGAGCGGTGCAGTGCACAAGGTGTCCCGAAAGAGGGAAGCGAAATAGGTTTTGTCTCCCTTGCATACGGAGATGGTATCCCAACACCTCACAGGAAGCCATAGCTGAGGCTTGTCCTGTGTGCCGCGGCAACTGCAACTGCACAGCCTGCCTTCGGTTGGATGCGCGTTTCAGGCGTTTCGAGAATCGAGACCCGAAAATTGATGAGGAGACCAAGCTGGAGCACTCTAAGTATTTGGTTCATCAGCTGCTCCCGTATTTGAAGATGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTGGAAGCCAAGAGGCAGGGGTTACGTGACCTTTCGGAGCTCAAGGTGGACAAGGCTAATAGCGGTGACGGAGTGAGAATGTACTGCAACAGCTGCAGCACTTCGATTTTTGATTTTCATAGGACTTGTCCCGGATGTGAGTATGATCTTTGCCTCGGTTGCTGCCGGGAGATTCGAGATGGTAAGTGGAAGAAGAGAGCTCAAGCGTCCATATTTGAGTGGAAATGGAGAGCAAGTGAAGATGGAAGCATTCTCTGCCCCCCAACACACATGAAAGGTTGTGGTAAGCATAATTTAGAATTGAGATGCCTTTTTCCTCAGAATCATGTTATGGAATTGGTAGAGAAAGCAGGAGAAATAGATGCTAGTTACAATTTTTTGCCTGATCGTAGTGAAACATGTGCATATAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTCAAGTAGTAGCAGCAGCTTGGCATTGGAAGAGGGGGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCTGTCATGTGGCGGGCATTTAGACAGATAAAGAAAATCAAGCATGATAGTGATTTGACAGTCCCCGCCATTGATTGCTGGAATTGGTGTTTTACAGAAATCAACCTCCTCCATTTCTTTAATGGCTATTGCAACGGACGGTCTTATGAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATCATCTACCGAGTTTCACAATCTTCTTCCCCGGCATGGTGAGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATACTCACCCCAACAACGGAACTCTGAACCTTGCTATCTACTTGCCACCTACCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTACACAGGAGCTTGAACGGGGAGATTCTGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTCAATATTCTGACACATTCAGCTGAAGTGGCCCTTACAACTAAACAACTGGCAACTATAGAGAAGTTGAAGAAAAAGCACAGGGAGCAAGACGAAAGAGAGATATTTGGGAATTGTCACACAAATGCTGGAAATGGAAGTGGGCCGCAGCAAGAATCAGCTGAAGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGATTCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCATGTCAATGAATTTATGCATGTGCTCTGTAATCCTTTGCAGCATGTTACTCATGCTATACATGATCAGACTGTTTATCTCACTGTAGAGCACAAGAGGAAGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTTCAAAGACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTTTCAAAGTTGCACTGGAGTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACAAACCACAGAGCCAGTGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTATTAAGGAGATTTCTGGTGAGAATGTGATGGTAAGGAGGGCAAGGGATGTGAAGGCAAAGAGGGCAAGGGCTCTGAAGGCAAAGAGGGCAAGGGCTCCCTTGGACGTGTCGACAATTTGCCATCAGTGTCAGAGAAATGTTAAGGGAAGAGTGGTGTGGTGCACATGGTGTGCGAGGAAGAGTTATTGTATTCCTTGCATACAGAGATGGTATCCCAACACATCAGAGGAAGCCATTGCTAAGGCATGTCCTGTGTGCCATGGCAACTGCAACTGCACAGCCTGCCTTCGATTGGATGTGCCTGTCAGGCGTTTGCAGAATCTAGACCTGAAAATCGACAAGGGTACCAAGCTGGAGCACTGTAAGTACTTGGTTCATCAGCTGCTTCCCTATTTGAAGAGGATTAGGGATGAGCAGGTGATTGAGATGCTGATCGAGGCTAAGAGGCAGGGATTACGTGATGTTTCGGAGCTCAAGGTGGAGAAGGCTAATTGCGGTGAAGATGAGCGCATGTACTGCAACAACTGCAGCACTTCGATTTTTGATTTTCATAGGAGTTGTCCTGAGTGTGAGTATGATCTGTGCCTCAGTTGCTGCCGGGAGATTCGAGATGGCAGGTGGAAGAAGAGAGGGATGGAGGAGAAAGATCAAGTGTCCACATTTGAGTGGAGAGCAAGTGAAGATGGAAGCATTCTCTGGACACATATGAAATGCTGTGGTAAGCATAAATTAGAATTGAAATGCAAATTTTCTCAGAATCATGTCATAGAATTGGTAGAGAAAGCAGGAAAAATAGATGAAAGTTACAGTCATTTGCGTGCTAGTAGTGAAACACCTGCACACAAGTGTTCATGTATTAAACCTGTGGATGATGCCGTTAGCTCAAGTAGCAGCTGCTCTATGTTAAGGAAAGCAGCTTCTAGAGATGGTTCTGATGATAACTACTTGTTCTGTCCAAGGGTTGGACACATCCAAGATAAGGATTTTGGGCATTTTCAGTGGCATTGGATGAGGGGTGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCTGTCATGTGGCGGGCATTTAGAAAGATAAAGAAGATCAAGCATGATAGTGAATTGACAGTCCACGCCATTGATTGCTGGGATTGGTGTTTTATAGAAATCAACATCCTCCATTTCTTTAGTGGATATTCCAACGGACGGTGTTATAAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATCATCTACTGAGTTTCACAATCGGCTTCCCCGGCATGTTAAGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATACTCACCCCAACAAGGGAACTCTGAACCTTGCTATCTACTTGCCACCTACCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACGGGGAGATTCTGTAACCCAGCTTCACTGCAATATGTCTGATGCGGTTAATATTCTGACGCATACAGCTGAAGTGCCCCTTACAACTGGACAACTGGCAACTATAGAGAAGTTGAAGAAAAAGCACAGGAAGCAAGACGAAAGAGAGATATTTGGGAATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCGCAGCAAGAATCCGCTGAAGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGATTCAGGATGTTCCGCTGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTATGCATGTGCTCTGTAATCCTTTGCAGCGTGTTACTCATGCTATACATGATCAGACTGTTTATCTCACTGTGGAGCACAAGAGGAAGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATTCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTGGAGTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACAAACCACAGAGCCAGTGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTGTGAAGGAGATTTCTGCTGAGAATGTGATGACTGAGGTTGTGAAACCTAGGGGAGGCACAAGGGAATTAAGGCAAGGTCTGCAAAAGCAGGGCCTTAAGACCATGAAGAAGATGGAGAAGTCAGTTTCGGACTTTGCTGGTATCATGAGGATTGTTGATATTTATAGGCTTGCAGTCATGAGGTTTAAGGGAGATATCGAGCTCTGGTTTCGACTTCATTACTTCATTGTAAGAACAACTGAAGTCCTGGCTGATGCAATCAAATTTCACCCAAAGGTCCCTGGTATCTGGATCTATGCTGCAGCTTGGGATTTTGACCATAATTTGAATGTTGAAGCTGCCCGTGCTATCATGCAAAATGGGTTAAGAGTTTGCGCAACTTCAGAAGACCTCTGGATAGAGTATCTTCGCATGGAACTCACATTCCTCAATAAATTAAAGGCTCGGAAGGTTGCTCTTGGAGAGGATGAGGGCACTTTTGCCCGTGATCAGATAAGTTTGGATAAGAAACAGTGGAGAGATGAGAACAAAGACTTACTCATGTCCCTTAATGACGAAGGAGAAAATAATGAAAGGTCAGATGATGAAAATGAAAAGTCAATAAAGAAAATTAGTAAGTTTCAAGAGCAAGGTTTTAGCATACTAAGAGCCATATATGCTGGAGCAGTAGAAGCTCTTCCCTCTAGTTTTAGTCTGAGAAAACCTTTTTTGGAGATCCTGGAAGCAACTGACTTGAAACCAGAGTATTGGGATTGGCTTGCAAGACTTAAATATAATCCTGCAAGTAGGGAAGATATTGGTGAAGATGTCACTCTTTCTCAGATAGAAAAAGCAGTTCAGGTTTACGAGGAGGCTATAGAAATTTTGCCTACGGCCATAATGCTCCACCAGTATGGGAAATTTTGGAATGGTGTTGTCACTCTTCTAAAACTAGAAAACAAGCCTTCTGGGTTAGCTAGTCCATCTGCTGGTTGTATTCCTCTTCTTTTGAGGGCTTACGAAAAGGTTAAAACAATGGGGTTATGGCTATTAAGAGTCTCTGTAGGTGTTAGACACTTTACAAGGGATTCCATTTCACCGAGTAAAGCTGACCTACTGTCCATCTTTGAACTTCTTAAAGATGTTTTGACAAGAGTGTCCATTTCAGAAGCTGAGAACCTGTGGTTAATGGCCCTTAAATTCTTTGCAAATCAAAAACATTACTTTGACAAGTTAGTTGAGATTTCTGTTAGATCATTGACAAAAAATGGCGGCAATGAGCTAGGATTTTCCCTCTCTGCTGCTATTGTGCATTTTGTTCTTCAAAAAGATGGAGTACTACATGCTAGAGAGATGTATAAACGCTTCATCCCTTTACCACACCCTGGACTTGCACTATATAGAGCCTGCATTGAATTGGAATCAAACCATGCATCCATTAGTGACAAAGATAGCCTCGTCAATGCCAGGAAATTATATGAATCTGCACTTAAAAACTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1916

Amino Acids

218.54

Weight (kDa)

8.26

Isoelectric Point (pI)

46.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 89 - 155 2.9e-06 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 585 - 680 2.1e-12 JmjC domain, hydroxylase
zf-4CXXC_R1 PF10497 760 - 820 6.4e-06 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 1281 - 1380 2e-12 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3390
AarI CACCTGC 4 cut(s) 577, 2575, 2775, 3013
AasI GACNNNNNNGTC 1 cut(s) 5404
Acc16I TGCGCA 1 cut(s) 4645
Acc36I ACCTGC 6 cut(s) 577, 1973, 2575, 2775, 3013, 4073
Acc65I GGTACC 1 cut(s) 2513
AccB1I GGYRCC 3 cut(s) 1748, 2513, 3848
AccB7I CCANNNNNTGG 3 cut(s) 3131, 5237, 5633
AccBSI CCGCTC 2 cut(s) 57, 311
AccI GTMKAC 3 cut(s) 1406, 2279, 3506
AccII CGCG 2 cut(s) 432, 472
AccIII TCCGGA 2 cut(s) 1767, 3867
AclWI GGATC 4 cut(s) 1129, 3229, 4568, 4978
AcoI YGGCCR 2 cut(s) 81, 5142
AcuI CTGAAG 8 cut(s) 237, 1611, 1749, 2264, 3711, 3849, 4530, 4635
AdeI CACNNNGTG 2 cut(s) 327, 2337
AfaI GTAC 5 cut(s) 686, 2515, 2537, 2684, 5586
AflII CTTAAG 1 cut(s) 4364
AflIII ACRYGT 2 cut(s) 1001, 2274
AhdI GACNNNNNGTC 1 cut(s) 5365
AjiI CACGTC 3 cut(s) 133, 2275, 3913
AjnI CCWGG 7 cut(s) 9, 161, 4515, 4549, 4986, 5632, 5714
AleI CACNNNNGTG 2 cut(s) 3013, 3789
AloI GAACNNNNNNTCC 2 cut(s) 4677, 4709
Alw26I GTCTC 5 cut(s) 365, 489, 509, 1286, 5311
Alw44I GTGCAC 3 cut(s) 244, 319, 2329
AlwI GGATC 4 cut(s) 1129, 3229, 4568, 4978
AlwNI CAGNNNCTG 2 cut(s) 2763, 5420
Ama87I CYCGRG 1 cut(s) 36
Aor13HI TCCGGA 2 cut(s) 1767, 3867
ApaLI GTGCAC 3 cut(s) 244, 319, 2329
Asp700I GAANNNNTTC 3 cut(s) 2085, 4185, 4944
Asp718I GGTACC 1 cut(s) 2513
AspA2I CCTAGG 1 cut(s) 4316
AspLEI GCGC 4 cut(s) 148, 472, 2679, 4646
AspS9I GGNCC 9 cut(s) 33, 1478, 1597, 1709, 3578, 3809, 4360, 4546, 5440
AsuC2I CCSGG 5 cut(s) 735, 770, 1363, 2768, 3463
AvaI CYCGRG 1 cut(s) 36
AvaII GGWCC 3 cut(s) 1478, 3578, 4546
AvrII CCTAGG 1 cut(s) 4316
BaeGI GKGCMC 5 cut(s) 248, 323, 2333, 3699, 4745
BaeI ACNNNNGTAYC 2 cut(s) 2497, 2530
BanI GGYRCC 3 cut(s) 1748, 2513, 3848
BanII GRGCYC 7 cut(s) 256, 645, 805, 2242, 2266, 2643, 4473
BarI GAAGNNNNNNTAC 8 cut(s) 1527, 1559, 3627, 3659, 4929, 4961, 5597, 5629
BbsI GAAGAC 2 cut(s) 135, 4662
BbvCI CCTCAGC 1 cut(s) 411
BceAI ACGGC 7 cut(s) 33, 124, 1028, 1921, 3029, 4021, 5157
BciT130I CCWGG 7 cut(s) 11, 163, 4517, 4551, 4988, 5634, 5716
BciVI GTATCC 2 cut(s) 393, 2391
BclI TGATCA 3 cut(s) 1873, 3973, 4756
BcnI CCSGG 5 cut(s) 735, 770, 1363, 2768, 3463
BcoDI GTCTC 5 cut(s) 365, 489, 509, 1286, 5311
BfoI RGCGCY 1 cut(s) 149
BfrI CTTAAG 1 cut(s) 4364
BfuAI ACCTGC 6 cut(s) 577, 1973, 2575, 2775, 3013, 4073
BfuI GTATCC 2 cut(s) 393, 2391
BglI GCCNNNNNGGC 2 cut(s) 20, 2476
BlnI CCTAGG 1 cut(s) 4316
BmcAI AGTACT 2 cut(s) 2537, 5586
Bme18I GGWCC 3 cut(s) 1478, 3578, 4546
BmeRI GACNNNNNGTC 1 cut(s) 5365
BmeT110I CYCGRG 1 cut(s) 36
BmgBI CACGTC 3 cut(s) 133, 2275, 3913
BmgT120I GGNCC 9 cut(s) 33, 1478, 1597, 1709, 3578, 3809, 4360, 4546, 5440
BoxI GACNNNNGTC 3 cut(s) 1476, 3576, 4046
BpiI GAAGAC 2 cut(s) 135, 4662
BpmI CTGGAG 9 cut(s) 545, 1811, 2054, 2150, 2543, 3911, 4154, 4250, 4950
Bpu10I CCTNAGC 2 cut(s) 411, 2409
BpuEI CTTGAG 9 cut(s) 629, 789, 1036, 1298, 1898, 2627, 3037, 3398, 3998
BpuMI CCSGG 5 cut(s) 735, 770, 1363, 2768, 3463
BsaAI YACGTR 2 cut(s) 629, 2627
BsaBI GATNNNNATC 1 cut(s) 2600
BsaI GGTCTC 2 cut(s) 489, 509
BsaWI WCCGGW 2 cut(s) 1767, 3867
Bse1I ACTGG 8 cut(s) 1620, 2037, 2115, 3712, 3720, 4137, 4215, 5158
Bse3DI GCAATG 4 cut(s) 1111, 2403, 3211, 5530
Bse8I GATNNNNATC 1 cut(s) 2600
BseAI TCCGGA 2 cut(s) 1767, 3867
BseBI CCWGG 7 cut(s) 11, 163, 4517, 4551, 4988, 5634, 5716
BseJI GATNNNNATC 1 cut(s) 2600
BseMI GCAATG 4 cut(s) 1111, 2403, 3211, 5530
BseNI ACTGG 8 cut(s) 1620, 2037, 2115, 3712, 3720, 4137, 4215, 5158
BseRI GAGGAG 4 cut(s) 527, 1241, 2822, 5132
BseSI GKGCMC 5 cut(s) 248, 323, 2333, 3699, 4745
BseX3I CGGCCG 1 cut(s) 81
BseYI CCCAGC 4 cut(s) 1134, 3234, 3321, 3639
BsgI GTGCAG 5 cut(s) 335, 430, 2428, 2991, 5718
Bsh1236I CGCG 2 cut(s) 432, 472
Bsh1285I CGRYCG 1 cut(s) 84
BshNI GGYRCC 3 cut(s) 1748, 2513, 3848
BsiEI CGRYCG 1 cut(s) 84
BsiHKCI CYCGRG 1 cut(s) 36
BsiSI CCGG 9 cut(s) 735, 769, 1363, 1469, 1768, 2767, 3463, 3569, 3868
BslFI GGGAC 7 cut(s) 315, 717, 1190, 1772, 3872, 4532, 4798
BsmAI GTCTC 5 cut(s) 365, 489, 509, 1286, 5311
BsmFI GGGAC 7 cut(s) 315, 717, 1190, 1772, 3872, 4532, 4798
BsmI GAATGC 2 cut(s) 852, 2859
Bso31I GGTCTC 2 cut(s) 489, 509
BsoBI CYCGRG 1 cut(s) 36
Bsp13I TCCGGA 2 cut(s) 1767, 3867
Bsp19I CCATGG 1 cut(s) 2428
BspEI TCCGGA 2 cut(s) 1767, 3867
BspFNI CGCG 2 cut(s) 432, 472
BspHI TCATGA 2 cut(s) 4413, 4446
BspMAI CTGCAG 5 cut(s) 701, 1984, 2699, 4084, 4573
BspMI ACCTGC 6 cut(s) 577, 1973, 2575, 2775, 3013, 4073
BspPI GGATC 4 cut(s) 1129, 3229, 4568, 4978
BspQI GCTCTTC 2 cut(s) 301, 4950
BspT107I GGYRCC 3 cut(s) 1748, 2513, 3848
BspTI CTTAAG 1 cut(s) 4364
BspTNI GGTCTC 2 cut(s) 489, 509
BsrBI CCGCTC 2 cut(s) 57, 311
BsrDI GCAATG 4 cut(s) 1111, 2403, 3211, 5530
BsrI ACTGG 8 cut(s) 1620, 2037, 2115, 3712, 3720, 4137, 4215, 5158
BssNAI GTATAC 2 cut(s) 1407, 3507
BssT1I CCWWGG 5 cut(s) 258, 2268, 2428, 3125, 4316
Bst1107I GTATAC 2 cut(s) 1407, 3507
Bst2UI CCWGG 7 cut(s) 11, 163, 4517, 4551, 4988, 5634, 5716
BstAFI CTTAAG 1 cut(s) 4364
BstAPI GCANNNNNTGC 7 cut(s) 453, 696, 858, 2451, 2685, 2694, 4348
BstBAI YACGTR 2 cut(s) 629, 2627
BstDSI CCRYGG 5 cut(s) 15, 430, 1937, 2428, 4037
BstENI CCTNNNNNAGG 2 cut(s) 166, 4320
BstFNI CGCG 2 cut(s) 432, 472
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 4 cut(s) 148, 472, 2679, 4646
BstMAI GTCTC 5 cut(s) 365, 489, 509, 1286, 5311
BstMCI CGRYCG 1 cut(s) 84
BstNI CCWGG 7 cut(s) 11, 163, 4517, 4551, 4988, 5634, 5716
BstPAI GACNNNNGTC 3 cut(s) 1476, 3576, 4046
BstSLI GKGCMC 5 cut(s) 248, 323, 2333, 3699, 4745
BstUI CGCG 2 cut(s) 432, 472
BstV2I GAAGAC 2 cut(s) 135, 4662
BstX2I RGATCY 2 cut(s) 4560, 4983
BstXI CCANNNNNNTGG 3 cut(s) 4058, 5016, 5162
BstYI RGATCY 2 cut(s) 4560, 4983
BstZ17I GTATAC 2 cut(s) 1407, 3507
BstZI CGGCCG 1 cut(s) 81
BsuI GTATCC 2 cut(s) 393, 2391
BtgI CCRYGG 5 cut(s) 15, 430, 1937, 2428, 4037
BtrI CACGTC 3 cut(s) 133, 2275, 3913
BtsI GCAGTG 3 cut(s) 323, 1546, 3646
BveI ACCTGC 6 cut(s) 577, 1973, 2575, 2775, 3013, 4073
CaiI CAGNNNCTG 2 cut(s) 2763, 5420
CciI TCATGA 2 cut(s) 4413, 4446
CfoI GCGC 4 cut(s) 148, 472, 2679, 4646
Cfr13I GGNCC 9 cut(s) 33, 1478, 1597, 1709, 3578, 3809, 4360, 4546, 5440
Cfr42I CCGCGG 1 cut(s) 433
CseI GACGC 2 cut(s) 798, 3688
Csp6I GTAC 5 cut(s) 685, 2514, 2536, 2683, 5585
CviQI GTAC 5 cut(s) 685, 2514, 2536, 2683, 5585
DraIII CACNNNGTG 2 cut(s) 327, 2337
DrdI GACNNNNNNGTC 1 cut(s) 5404
DriI GACNNNNNGTC 1 cut(s) 5365
DseDI GACNNNNNNGTC 1 cut(s) 5404
EaeI YGGCCR 2 cut(s) 81, 5142
EagI CGGCCG 1 cut(s) 81
Eam1105I GACNNNNNGTC 1 cut(s) 5365
EciI GGCGGA 1 cut(s) 193
Ecl136II GAGCTC 4 cut(s) 643, 803, 2641, 4471
EclXI CGGCCG 1 cut(s) 81
Eco130I CCWWGG 5 cut(s) 258, 2268, 2428, 3125, 4316
Eco24I GRGCYC 7 cut(s) 256, 645, 805, 2242, 2266, 2643, 4473
Eco31I GGTCTC 2 cut(s) 489, 509
Eco32I GATATC 1 cut(s) 4465
Eco47I GGWCC 3 cut(s) 1478, 3578, 4546
Eco52I CGGCCG 1 cut(s) 81
Eco53kI GAGCTC 4 cut(s) 643, 803, 2641, 4471
Eco57I CTGAAG 8 cut(s) 237, 1611, 1749, 2264, 3711, 3849, 4530, 4635
Eco88I CYCGRG 1 cut(s) 36
EcoICRI GAGCTC 4 cut(s) 643, 803, 2641, 4471
EcoNI CCTNNNNNAGG 2 cut(s) 166, 4320
EcoO109I RGGNCCY 3 cut(s) 33, 4360, 4546
EcoRI GAATTC 4 cut(s) 5, 99, 1922, 4022
EcoRII CCWGG 7 cut(s) 9, 161, 4515, 4549, 4986, 5632, 5714
EcoRV GATATC 1 cut(s) 4465
EcoT14I CCWWGG 5 cut(s) 258, 2268, 2428, 3125, 4316
EcoT22I ATGCAT 3 cut(s) 1830, 3930, 5683
EcoT38I GRGCYC 7 cut(s) 256, 645, 805, 2242, 2266, 2643, 4473
ErhI CCWWGG 5 cut(s) 258, 2268, 2428, 3125, 4316
FalI AAGNNNNNCTT 4 cut(s) 1584, 1616, 3684, 3716
FaqI GGGAC 7 cut(s) 315, 717, 1190, 1772, 3872, 4532, 4798
FauI CCCGC 3 cut(s) 1149, 1216, 3249
FauNDI CATATG 1 cut(s) 2874
FbaI TGATCA 3 cut(s) 1873, 3973, 4756
FblI GTMKAC 3 cut(s) 1406, 2279, 3506
FriOI GRGCYC 7 cut(s) 256, 645, 805, 2242, 2266, 2643, 4473
FspI TGCGCA 1 cut(s) 4645
GlaI GCGC 4 cut(s) 147, 471, 2678, 4645
GsaI CCCAGC 4 cut(s) 1138, 3238, 3325, 3643
GsuI CTGGAG 9 cut(s) 545, 1811, 2054, 2150, 2543, 3911, 4154, 4250, 4950
HaeII RGCGCY 1 cut(s) 149
HapII CCGG 9 cut(s) 735, 769, 1363, 1469, 1768, 2767, 3463, 3569, 3868
HgaI GACGC 2 cut(s) 798, 3688
HhaI GCGC 4 cut(s) 148, 472, 2679, 4646
Hin6I GCGC 4 cut(s) 146, 470, 2677, 4644
HinP1I GCGC 4 cut(s) 146, 470, 2677, 4644
HincII GTYRAC 1 cut(s) 2280
HindII GTYRAC 1 cut(s) 2280
HindIII AAGCTT 2 cut(s) 152, 1541
HpaII CCGG 9 cut(s) 735, 769, 1363, 1469, 1768, 2767, 3463, 3569, 3868
Hpy99I CGWCG 1 cut(s) 116
HpyCH4IV ACGT 5 cut(s) 132, 628, 2274, 2626, 3912
HpySE526I ACGT 5 cut(s) 132, 628, 2274, 2626, 3912
HspAI GCGC 4 cut(s) 146, 470, 2677, 4644
Kpn2I TCCGGA 2 cut(s) 1767, 3867
KpnI GGTACC 1 cut(s) 2517
Ksp22I TGATCA 3 cut(s) 1873, 3973, 4756
KspI CCGCGG 1 cut(s) 433
LguI GCTCTTC 2 cut(s) 301, 4950
MaeII ACGT 5 cut(s) 132, 628, 2274, 2626, 3912
MbiI CCGCTC 2 cut(s) 57, 311
MfeI CAATTG 1 cut(s) 120
MflI RGATCY 2 cut(s) 4560, 4983
MlyI GAGTC 1 cut(s) 5313
MmeI TCCRAC 5 cut(s) 444, 2123, 3112, 3399, 4223
Mph1103I ATGCAT 3 cut(s) 1830, 3930, 5683
MroI TCCGGA 2 cut(s) 1767, 3867
MroXI GAANNNNTTC 3 cut(s) 2085, 4185, 4944
MspCI CTTAAG 1 cut(s) 4364
MspI CCGG 9 cut(s) 735, 769, 1363, 1469, 1768, 2767, 3463, 3569, 3868
MunI CAATTG 1 cut(s) 120
Mva1269I GAATGC 2 cut(s) 852, 2859
MvaI CCWGG 7 cut(s) 11, 163, 4517, 4551, 4988, 5634, 5716
MvnI CGCG 2 cut(s) 432, 472
NciI CCSGG 5 cut(s) 735, 770, 1363, 2768, 3463
NcoI CCATGG 1 cut(s) 2428
NdeI CATATG 1 cut(s) 2874
NmeAIII GCCGAG 2 cut(s) 78, 109
NmuCI GTSAC 7 cut(s) 629, 668, 1682, 3782, 5077, 5185, 5690
NsbI TGCGCA 1 cut(s) 4645
NsiI ATGCAT 3 cut(s) 1830, 3930, 5683
OliI CACNNNNGTG 2 cut(s) 3013, 3789
PaeI GCATGC 2 cut(s) 2156, 4256
PagI TCATGA 2 cut(s) 4413, 4446
PaqCI CACCTGC 4 cut(s) 577, 2575, 2775, 3013
PasI CCCWGGG 1 cut(s) 162
PciI ACATGT 1 cut(s) 1001
PciSI GCTCTTC 2 cut(s) 301, 4950
PctI GAATGC 2 cut(s) 852, 2859
PdmI GAANNNNTTC 3 cut(s) 2085, 4185, 4944
PflMI CCANNNNNTGG 3 cut(s) 3131, 5237, 5633
PfoI TCCNGGA 2 cut(s) 733, 4986
PleI GAGTC 1 cut(s) 5312
PpsI GAGTC 1 cut(s) 5312
Ppu21I YACGTR 2 cut(s) 629, 2627
PpuMI RGGWCCY 1 cut(s) 4546
PscI ACATGT 1 cut(s) 1001
PshAI GACNNNNGTC 3 cut(s) 1476, 3576, 4046
PsiI TTATAA 1 cut(s) 3390
Psp124BI GAGCTC 4 cut(s) 645, 805, 2643, 4473
Psp5II RGGWCCY 1 cut(s) 4546
Psp6I CCWGG 7 cut(s) 9, 161, 4515, 4549, 4986, 5632, 5714
PspFI CCCAGC 4 cut(s) 1134, 3234, 3321, 3639
PspGI CCWGG 7 cut(s) 9, 161, 4515, 4549, 4986, 5632, 5714
PspPI GGNCC 9 cut(s) 33, 1478, 1597, 1709, 3578, 3809, 4360, 4546, 5440
PspPPI RGGWCCY 1 cut(s) 4546
PstI CTGCAG 5 cut(s) 701, 1984, 2699, 4084, 4573
PstNI CAGNNNCTG 2 cut(s) 2763, 5420
PsuI RGATCY 2 cut(s) 4560, 4983
PvuII CAGCTG 8 cut(s) 553, 696, 1589, 1727, 1789, 2551, 3063, 3689
RsaI GTAC 5 cut(s) 686, 2515, 2537, 2684, 5586
RsaNI GTAC 5 cut(s) 685, 2514, 2536, 2683, 5585
SacI GAGCTC 4 cut(s) 645, 805, 2643, 4473
SacII CCGCGG 1 cut(s) 433
SalI GTCGAC 1 cut(s) 2278
SapI GCTCTTC 2 cut(s) 301, 4950
Sau96I GGNCC 9 cut(s) 33, 1478, 1597, 1709, 3578, 3809, 4360, 4546, 5440
SbfI CCTGCAGG 2 cut(s) 1984, 4084
ScaI AGTACT 2 cut(s) 2537, 5586
SchI GAGTC 1 cut(s) 5313
SdaI CCTGCAGG 2 cut(s) 1984, 4084
Sfr303I CCGCGG 1 cut(s) 433
SgrBI CCGCGG 1 cut(s) 433
SinI GGWCC 3 cut(s) 1478, 3578, 4546
SphI GCATGC 2 cut(s) 2156, 4256
Sse8387I CCTGCAGG 2 cut(s) 1984, 4084
SspI AATATT 2 cut(s) 1573, 3673
SstI GAGCTC 4 cut(s) 645, 805, 2643, 4473
StyI CCWWGG 5 cut(s) 258, 2268, 2428, 3125, 4316
TaiI ACGT 5 cut(s) 135, 631, 2277, 2629, 3915
TatI WGTACW 4 cut(s) 684, 2535, 2682, 5584
TseFI GTSAC 7 cut(s) 629, 668, 1682, 3782, 5077, 5185, 5690
Tsp45I GTSAC 7 cut(s) 629, 668, 1682, 3782, 5077, 5185, 5690
TspGWI ACGGA 8 cut(s) 129, 388, 687, 1293, 1437, 2093, 3393, 4193
Van91I CCANNNNNTGG 3 cut(s) 3131, 5237, 5633
Vha464I CTTAAG 1 cut(s) 4364
VneI GTGCAC 3 cut(s) 244, 319, 2329
VpaK11BI GGWCC 3 cut(s) 1478, 3578, 4546
XagI CCTNNNNNAGG 2 cut(s) 166, 4320
XbaI TCTAGA 2 cut(s) 2491, 3089
XcmI CCANNNNNNNNNTGG 5 cut(s) 1149, 1262, 3249, 3362, 4378
XmaJI CCTAGG 1 cut(s) 4316
XmiI GTMKAC 3 cut(s) 1406, 2279, 3506
XmnI GAANNNNTTC 3 cut(s) 2085, 4185, 4944
ZrmI AGTACT 2 cut(s) 2537, 5586
Zsp2I ATGCAT 3 cut(s) 1830, 3930, 5683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.