Rroxscaffold_3G00235160

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000280
Physical Location & Seq
Reverse (-)
22000817 .. 22005520
4704 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00235160.1

Sequence Viewer

Length: 4704 bp
ATGAGAATCCAGGCGACGCCGTCGAGATTGTGGCCCCGAGGCACAAAGCGGCCGAACATATGGGGAATTCAGTACGACGACGGAACAAATGGGGAAGAAGATGGTGGAGGACCGTCTGTGGAGCCACTGCCAAAGCTTGTTCCCTGGGGGAGGAAGCGGCGGAGGCTAGGATGCGAGGGTTTTTGGGCAAATCAAAATGAAGGAAAGGCAAGGGCTGTGAAGGCGAATAAGAAGAAGAAGAAGAAGCAGCAGCAGCAGAGAAGGGCTCCCTTGGAGGTGCAGCAGCAGCAGAGTAGGGATAAACAAGTACCACTACCTAGCTACCTTCAGAGAAGGGCTCCCTTGGAGGTGCAGCAGCAGAGCAGGGACAAACAAGAACCACTACCTAGCTACCTGCAGAGAAGGGCTCCCTTGAAGGTACGGACAATCAAGTGCCATCAATGTAAGCAAAATGATATGGGAAGAGCGGTGCGTTGCACAAAGTGTTCGAGGAAGAGGTATTGTATCCCTTGCATAAGGACTTGGTATCCCAACACCTCAGAGGAAGCCATAGCTGAGGCTTGTCCTGCGTGCCGCGGCAACTGCAACTGCAATGTCTGCCTTTGCTGGGGCGTGCCTGTCAGGAATCTGAACCCCAAAATCGACAAGGAGACCAAGCTGGAACACTCTAAGTATTTGGTTCATCAGCTGCTTCCGTATTTGAAGATGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTGGAAGCCAAGAGGCAGGGGTTACGTGACCTTTCGGAGCTCAAGGTGGACAAGGCTAAAAGCGGTGGTGACCAAGTGCGTTTGTACTGCAACAGCTGCAGCACTTCGATTTTTGATTTTCATAGGACTTGTCCCGGGTGTGAGTATGATCTGTGCCTAGCCTGCTGCCGGGAGATTCGAGATGGCAAGTGGAAGAAGAGAGCTCAAGCGTCCACATTTGAGTGGAAATGGAGAGCAAGTGAAGACGGAAACATTTTCTGCCCACCAACACACATGAAAGGTTGTGGTAAGCATAATTTAGAATTGAGATGCATTTTTCCTCAGAATCATGTCATGGAGTTGGTAGAGAAAGCAGAAGAAATAGATGCAAGTTACAATTTTTTGCCTGATTGTAGTGAAACATGTGCATATAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTCAAGTAGTAGCAGCAGCTGTAAGTTAAGGAAAGCAGCTTCTAGAGTTGCTTCTGATAGTGACTACTTGTTCTGTCCAAGGGTTGGACAAATTCAAGATAAGGATTTTGGGCATTTTCAGTGGCATTGGAAGAGGGGCGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCAGGTTTGAGCTGGGAACCATCTGTCATGTGGCGGGCATTTAGACGGATAGAGAAAGTCAAGAATGATAGTGAATTGACATTCCACGCCATTGATTGTTGGGATTGGTGTTCTATAGAAATCAACATCCTCCATTTCTTTAGTGGATATTCCAACGGACGGTCTCATAAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATCATCTGCTGAATTTTACAAACGTCTTCCCCGGCATTGTAAGGAATTTTTATGTTGTCTGCCTCTCAAGGAGTATACTCACCCCAACAAGGGAACTTTGAACCTTGCTACCTACTTGCCACCTACCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACAGGGAGATTCCGTAACCAAGCTTCACTGCAATAGGTCTGATGCGGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACAACTGAACAACTTGCAACTATAGAGAAGTTGAAGAAAAAGCACAGGGAGCAAAATGAAAGAGAGGTATTTGGGGATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCTCAGCAAGAGTCAGCTATAGAGAAGTTGAAGAGAAAGTATAGGGAGCAAAACGAAAGAGAGGTATTTGGGAATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCTCAGGAAGAGTCAGCTGAAGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGATCCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCATGTCAATGAATTTATGCATGTACTTGGTAATCCTTTGCAGCATGTTACTCATGCTATACATGATCAGACTGTCTATCTCACTGTGGAACACAAGAGGAAGCTCAAGGAGGAATTTGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTGGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTCTGACGGAAGAGTTTCGCGCACTTCCGACAAACCACAGAGCCCATGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTATGAAGGAGATTTCTGGTGAGAATGTGACAATGAGGGTAAGGACTGTGAAGGCAAAGAGGGCAAGGGACGAGTCGATACACTGCCATCAGTGTCATAGATATGTTATGGGAAGAGTAGTGCAGTGCACAAGGTGTCTGGAAAGAGGTACGGGAATGAGGACGAGGTATTGTATCCCTTGTATACAGAGATGGTATCCCAACACATCAGAGGAAGCTATTGCTAAGGCTTGTCCTGTATGCCACGGCAACTGCAACTGCACAGCCTGCCTTCGTTTGGATGTGCCTGGCAGGTGTTTGCAGAATCTAGACCCCAAAATCGACAGGGGTACCAAGCCGGAGCACTGTAAGTATTTGGTTCATCAGCTGCTTCCCTATTTGAAGAGGATTAGGGATGAGCAGGTGAGTGAGATGATGGTCGAGGCTAAGAGGCGGGGATTACGTGATGTTTTGGAGCTCAAGGTGGAGAAGGCTAAGTGCGGTGAACATGAGCGTATGTACTGCAACAACTGCCGCACTTCAATTGTTGATTTTCATAGGAGTTGTCCCAAGTGTGGGTATGATCTGTGCCTCAGTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAAGAAGAGAGAGGTGGAGGAAGCACGAGTGTCCACATTTGAGTGGAGATTTAGTGAAGATGGAAGCATTCTCTGCCCGCCAACACAAAAGAAATGTTTTGGTAAGCATAATTTAGAATTGAGATGCATTTTTTCTCAGAATCATGTCATGGAATTGGTGGAGAAAGCAGGAAAAATAGATGCGAGTTACAATTTTTTGCCTGATCGTAGTGAAACATGTGCATACAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTCAGCTCAAGCAGCAGCAGCTGTAAATTAAGGAAAGCAGCTTCTAGAGTTGCTTCTGATAGTAACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAAGATAAGGATTTTGGACATTTTCAGTGGCATTGGATGAGGGGCGAGCCTGTGGTCGTTAGCAATGCATTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCCGTCATGTGGCGGGCACTTAGAAAGACAGTTAATAACTACCATGCTAGTGACTTGACATTCCACGCCGTTGATTGCTGGGATTGGTGTTTTATAAGAATCAAAATCCGCCATTTCTTTAGTGGATATTCCAAAGGACGGTCTTATAAGACAGGATGGCCTCAGAATCTCAAGGTCAATGACTTCCCATTATCTACTGAGTTTCACAATCGTCTTCCCCGGCATGGTAGGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATACTCACCCCAACAAGGGAACTCTGAACCTTGCTATCTACTTGCCACCTCTCTCAGTAAAGCCAGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACAGGGAGATTCGGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTTGATATTCTGACACATACAGCTGAAGTGGCCCTTACTACTGAACAACTTGCAACTATAGAGAAGTTGAAGAAAAAGCACAGGAAGCAAGACAAAAGAGAGATATTTGGGAATTGTCACTCAAGTGCTGGAAATGGAAGTGGGTCTCAGGAAGAATTAGCTGATGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGACTCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTATGCATGTGCTCTGTAATCCTTTGCAGCATGTTACTCATGCTATACATGATCAGACTGTTTATCTCACTGTGGAGCACAAGAGGAGGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTGTGAAAGTTGCACTGGACTTTGTCTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACAAACCATAGAGCCAGTGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTGTGAAGGAGATTTCTGGTGAGAATGTGATGACTGAGGATGCAACGGCTAGGGGAGGCGCAATGGAATTAAGGCAGGGTGTAAGGCCCAAATCTTACAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1567

Amino Acids

179.35

Weight (kDa)

8.77

Isoelectric Point (pI)

49.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 142 - 201 6.3e-07 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 702 - 795 9.5e-13 JmjC domain, hydroxylase
zf-4CXXC_R1 PF10497 862 - 929 7.8e-06 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 1394 - 1490 3.2e-13 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3668, 3720
AarI CACCTGC 3 cut(s) 712, 2793, 2902
Acc36I ACCTGC 6 cut(s) 402, 712, 2318, 2793, 2902, 4403
Acc65I GGTACC 1 cut(s) 2840
AccB1I GGYRCC 3 cut(s) 2093, 2840, 4178
AccB7I CCANNNNNTGG 2 cut(s) 1271, 3461
AccBSI CCGCTC 1 cut(s) 467
AccI GTMKAC 3 cut(s) 1646, 2695, 3836
AccII CGCG 2 cut(s) 576, 2436
AccIII TCCGGA 2 cut(s) 2112, 4197
AclWI GGATC 4 cut(s) 1369, 2110, 2123, 3559
AcoI YGGCCR 1 cut(s) 50
AcsI RAATTY 9 cut(s) 66, 1278, 1583, 1616, 2165, 2267, 3806, 4250, 4352
AcuI CTGAAG 4 cut(s) 311, 1851, 2094, 4041
AcyI GRCGYC 1 cut(s) 17
AdeI CACNNNGTG 2 cut(s) 483, 2646
AfaI GTAC 8 cut(s) 74, 309, 420, 824, 2178, 2662, 2842, 3011
AflIII ACRYGT 2 cut(s) 1139, 3332
AjiI CACGTC 1 cut(s) 4243
AjnI CCWGG 4 cut(s) 9, 143, 2118, 2797
AleI CACNNNNGTG 3 cut(s) 1929, 2034, 4119
Alw21I GWGCWC 7 cut(s) 780, 943, 2642, 2856, 2970, 4266, 4332
Alw26I GTCTC 4 cut(s) 644, 1530, 4146, 4477
Alw44I GTGCAC 1 cut(s) 2638
AlwI GGATC 4 cut(s) 1369, 2110, 2123, 3559
AlwNI CAGNNNCTG 3 cut(s) 1206, 3090, 3396
Ama87I CYCGRG 2 cut(s) 36, 872
Aor13HI TCCGGA 2 cut(s) 2112, 4197
AoxI GGCC 9 cut(s) 32, 50, 1541, 1836, 1949, 2054, 3731, 4026, 4682
ApaLI GTGCAC 1 cut(s) 2638
ApoI RAATTY 9 cut(s) 66, 1278, 1583, 1616, 2165, 2267, 3806, 4250, 4352
Asp700I GAANNNNTTC 2 cut(s) 2430, 4515
Asp718I GGTACC 1 cut(s) 2840
AspLEI GCGC 2 cut(s) 2438, 4658
AspS9I GGNCC 9 cut(s) 33, 110, 1718, 1837, 1949, 2054, 3908, 4027, 4683
AsuC2I CCSGG 6 cut(s) 873, 874, 908, 1603, 3095, 3793
AvaI CYCGRG 2 cut(s) 36, 872
AvaII GGWCC 3 cut(s) 110, 1718, 3908
AxyI CCTNAGG 1 cut(s) 2058
BaeGI GKGCMC 2 cut(s) 2642, 3592
BaeI ACNNNNGTAYC 2 cut(s) 2824, 2857
BamHI GGATCC 1 cut(s) 2115
BanI GGYRCC 3 cut(s) 2093, 2840, 4178
BanII GRGCYC 7 cut(s) 268, 340, 409, 780, 943, 2461, 2970
BarI GAAGNNNNNNTAC 4 cut(s) 1767, 1799, 3957, 3989
BauI CACGAG 1 cut(s) 3141
BbsI GAAGAC 3 cut(s) 987, 1589, 3779
Bbv12I GWGCWC 7 cut(s) 780, 943, 2642, 2856, 2970, 4266, 4332
BbvCI CCTCAGC 2 cut(s) 555, 1953
BceAI ACGGC 8 cut(s) 4, 1166, 2266, 2773, 3359, 3626, 4351, 4659
BciT130I CCWGG 4 cut(s) 11, 145, 2120, 2799
BciVI GTATCC 4 cut(s) 515, 537, 2696, 2718
BclI TGATCA 2 cut(s) 2218, 4303
BcnI CCSGG 6 cut(s) 873, 874, 908, 1603, 3095, 3793
BcoDI GTCTC 4 cut(s) 644, 1530, 4146, 4477
BfaI CTAG 9 cut(s) 167, 318, 387, 896, 1230, 2819, 3420, 3621, 4647
BfmI CTRYAG 8 cut(s) 395, 835, 1476, 1863, 1968, 2325, 4053, 4410
BfuAI ACCTGC 6 cut(s) 402, 712, 2318, 2793, 2902, 4403
BfuI GTATCC 4 cut(s) 515, 537, 2696, 2718
Bme18I GGWCC 3 cut(s) 110, 1718, 3908
BmeT110I CYCGRG 2 cut(s) 36, 872
BmgBI CACGTC 1 cut(s) 4243
BmgT120I GGNCC 9 cut(s) 33, 110, 1718, 1837, 1949, 2054, 3908, 4027, 4683
BoxI GACNNNNGTC 4 cut(s) 1716, 2291, 3906, 4376
BpiI GAAGAC 3 cut(s) 987, 1589, 3779
BplI GAGNNNNNCTC 6 cut(s) 250, 282, 322, 354, 391, 423
BpmI CTGGAG 4 cut(s) 2156, 2495, 4241, 4580
Bpu10I CCTNAGC 3 cut(s) 555, 1953, 2736
BpuMI CCSGG 6 cut(s) 873, 874, 908, 1603, 3095, 3793
BsaAI YACGTR 2 cut(s) 764, 2954
BsaHI GRCGYC 1 cut(s) 17
BsaI GGTCTC 3 cut(s) 644, 1530, 4146
BsaWI WCCGGW 2 cut(s) 2112, 4197
Bse1I ACTGG 3 cut(s) 2382, 4467, 4545
Bse21I CCTNAGG 1 cut(s) 2058
Bse3DI GCAATG 4 cut(s) 598, 1351, 3541, 4665
BseAI TCCGGA 2 cut(s) 2112, 4197
BseBI CCWGG 4 cut(s) 11, 145, 2120, 2799
BseMI GCAATG 4 cut(s) 598, 1351, 3541, 4665
BseNI ACTGG 3 cut(s) 2382, 4467, 4545
BseRI GAGGAG 1 cut(s) 4351
BseSI GKGCMC 2 cut(s) 2642, 3592
BseX3I CGGCCG 1 cut(s) 50
BseYI CCCAGC 4 cut(s) 606, 1374, 3564, 3651
BsgI GTGCAG 4 cut(s) 299, 371, 2654, 2755
Bsh1236I CGCG 2 cut(s) 576, 2436
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 9 cut(s) 34, 52, 1543, 1838, 1951, 2056, 3733, 4028, 4684
BshNI GGYRCC 3 cut(s) 2093, 2840, 4178
BsiEI CGRYCG 1 cut(s) 53
BsiHKAI GWGCWC 7 cut(s) 780, 943, 2642, 2856, 2970, 4266, 4332
BsiHKCI CYCGRG 2 cut(s) 36, 872
BsiSI CCGG 9 cut(s) 873, 907, 1603, 1709, 2113, 2849, 3094, 3793, 4198
BslFI GGGAC 6 cut(s) 380, 855, 2117, 2594, 3042, 4202
BsmAI GTCTC 4 cut(s) 644, 1530, 4146, 4477
BsmFI GGGAC 6 cut(s) 380, 855, 2117, 2594, 3042, 4202
BsmI GAATGC 1 cut(s) 3183
BsnI GGCC 9 cut(s) 34, 52, 1543, 1838, 1951, 2056, 3733, 4028, 4684
Bso31I GGTCTC 3 cut(s) 644, 1530, 4146
BsoBI CYCGRG 2 cut(s) 36, 872
Bsp13I TCCGGA 2 cut(s) 2112, 4197
Bsp143I GATC 9 cut(s) 886, 1335, 1361, 2115, 2218, 3073, 3319, 3551, 4303
BspANI GGCC 9 cut(s) 34, 52, 1543, 1838, 1951, 2056, 3733, 4028, 4684
BspEI TCCGGA 2 cut(s) 2112, 4197
BspFNI CGCG 2 cut(s) 576, 2436
BspMAI CTGCAG 4 cut(s) 399, 839, 2329, 4414
BspMI ACCTGC 6 cut(s) 402, 712, 2318, 2793, 2902, 4403
BspPI GGATC 4 cut(s) 1369, 2110, 2123, 3559
BspQI GCTCTTC 1 cut(s) 457
BspT107I GGYRCC 3 cut(s) 2093, 2840, 4178
BspTNI GGTCTC 3 cut(s) 644, 1530, 4146
BsrBI CCGCTC 1 cut(s) 467
BsrDI GCAATG 4 cut(s) 598, 1351, 3541, 4665
BsrI ACTGG 3 cut(s) 2382, 4467, 4545
BssMI GATC 9 cut(s) 886, 1335, 1361, 2115, 2218, 3073, 3319, 3551, 4303
BssNAI GTATAC 3 cut(s) 1647, 2696, 3837
BssNI GRCGYC 1 cut(s) 17
BssSI CACGAG 1 cut(s) 3141
BssT1I CCWWGG 4 cut(s) 270, 342, 1265, 3455
Bst1107I GTATAC 3 cut(s) 1647, 2696, 3837
Bst2BI CACGAG 1 cut(s) 3141
Bst2UI CCWGG 4 cut(s) 11, 145, 2120, 2799
BstACI GRCGYC 1 cut(s) 17
BstAPI GCANNNNNTGC 5 cut(s) 597, 834, 2778, 3021, 3189
BstBAI YACGTR 2 cut(s) 764, 2954
BstDSI CCRYGG 4 cut(s) 574, 2282, 2755, 4367
BstEII GGTNACC 2 cut(s) 806, 3964
BstENI CCTNNNNNAGG 1 cut(s) 148
BstFNI CGCG 2 cut(s) 576, 2436
BstHHI GCGC 2 cut(s) 2438, 4658
BstKTI GATC 9 cut(s) 889, 1338, 1364, 2118, 2221, 3076, 3322, 3554, 4306
BstMAI GTCTC 4 cut(s) 644, 1530, 4146, 4477
BstMBI GATC 9 cut(s) 886, 1335, 1361, 2115, 2218, 3073, 3319, 3551, 4303
BstMCI CGRYCG 1 cut(s) 53
BstNI CCWGG 4 cut(s) 11, 145, 2120, 2799
BstNSI RCATGY 9 cut(s) 1143, 2159, 2177, 2201, 2501, 3336, 4262, 4286, 4586
BstPAI GACNNNNGTC 4 cut(s) 1716, 2291, 3906, 4376
BstPI GGTNACC 2 cut(s) 806, 3964
BstSFI CTRYAG 8 cut(s) 395, 835, 1476, 1863, 1968, 2325, 4053, 4410
BstSLI GKGCMC 2 cut(s) 2642, 3592
BstUI CGCG 2 cut(s) 576, 2436
BstV2I GAAGAC 3 cut(s) 987, 1589, 3779
BstX2I RGATCY 1 cut(s) 2115
BstXI CCANNNNNNTGG 2 cut(s) 2303, 4388
BstYI RGATCY 1 cut(s) 2115
BstZ17I GTATAC 3 cut(s) 1647, 2696, 3837
BstZI CGGCCG 1 cut(s) 50
Bsu36I CCTNAGG 1 cut(s) 2058
BsuI GTATCC 4 cut(s) 515, 537, 2696, 2718
BsuRI GGCC 9 cut(s) 34, 52, 1543, 1838, 1951, 2056, 3733, 4028, 4684
BtgI CCRYGG 4 cut(s) 574, 2282, 2755, 4367
BtrI CACGTC 1 cut(s) 4243
BtsI GCAGTG 5 cut(s) 125, 1786, 2593, 2642, 3976
BveI ACCTGC 6 cut(s) 402, 712, 2318, 2793, 2902, 4403
CaiI CAGNNNCTG 3 cut(s) 1206, 3090, 3396
CfoI GCGC 2 cut(s) 2438, 4658
Cfr13I GGNCC 9 cut(s) 33, 110, 1718, 1837, 1949, 2054, 3908, 4027, 4683
Cfr42I CCGCGG 1 cut(s) 577
Cfr9I CCCGGG 1 cut(s) 872
CseI GACGC 2 cut(s) 25, 936
Csp6I GTAC 8 cut(s) 73, 308, 419, 823, 2177, 2661, 2841, 3010
CviQI GTAC 8 cut(s) 73, 308, 419, 823, 2177, 2661, 2841, 3010
DpnI GATC 9 cut(s) 888, 1337, 1363, 2117, 2220, 3075, 3321, 3553, 4305
DpnII GATC 9 cut(s) 886, 1335, 1361, 2115, 2218, 3073, 3319, 3551, 4303
DraIII CACNNNGTG 2 cut(s) 483, 2646
EaeI YGGCCR 1 cut(s) 50
EagI CGGCCG 1 cut(s) 50
EciI GGCGGA 2 cut(s) 175, 3671
Ecl136II GAGCTC 3 cut(s) 778, 941, 2968
EclXI CGGCCG 1 cut(s) 50
Eco130I CCWWGG 4 cut(s) 270, 342, 1265, 3455
Eco24I GRGCYC 7 cut(s) 268, 340, 409, 780, 943, 2461, 2970
Eco31I GGTCTC 3 cut(s) 644, 1530, 4146
Eco47I GGWCC 3 cut(s) 110, 1718, 3908
Eco52I CGGCCG 1 cut(s) 50
Eco53kI GAGCTC 3 cut(s) 778, 941, 2968
Eco57I CTGAAG 4 cut(s) 311, 1851, 2094, 4041
Eco81I CCTNAGG 1 cut(s) 2058
Eco88I CYCGRG 2 cut(s) 36, 872
Eco91I GGTNACC 2 cut(s) 806, 3964
EcoICRI GAGCTC 3 cut(s) 778, 941, 2968
EcoNI CCTNNNNNAGG 1 cut(s) 148
EcoO65I GGTNACC 2 cut(s) 806, 3964
EcoRI GAATTC 2 cut(s) 66, 4352
EcoRII CCWGG 4 cut(s) 9, 143, 2118, 2797
EcoT14I CCWWGG 4 cut(s) 270, 342, 1265, 3455
EcoT22I ATGCAT 5 cut(s) 1052, 2175, 3245, 3541, 4260
EcoT38I GRGCYC 7 cut(s) 268, 340, 409, 780, 943, 2461, 2970
ErhI CCWWGG 4 cut(s) 270, 342, 1265, 3455
FalI AAGNNNNNCTT 4 cut(s) 1824, 1856, 4014, 4046
FaqI GGGAC 6 cut(s) 380, 855, 2117, 2594, 3042, 4202
FauI CCCGC 4 cut(s) 1389, 2937, 3201, 3579
FauNDI CATATG 1 cut(s) 59
FbaI TGATCA 2 cut(s) 2218, 4303
FblI GTMKAC 3 cut(s) 1646, 2695, 3836
FriOI GRGCYC 7 cut(s) 268, 340, 409, 780, 943, 2461, 2970
FspBI CTAG 9 cut(s) 167, 318, 387, 896, 1230, 2819, 3420, 3621, 4647
GlaI GCGC 2 cut(s) 2437, 4657
GsaI CCCAGC 4 cut(s) 610, 1378, 3568, 3655
GsuI CTGGAG 4 cut(s) 2156, 2495, 4241, 4580
HaeIII GGCC 9 cut(s) 34, 52, 1543, 1838, 1951, 2056, 3733, 4028, 4684
HapII CCGG 9 cut(s) 873, 907, 1603, 1709, 2113, 2849, 3094, 3793, 4198
HgaI GACGC 2 cut(s) 25, 936
HhaI GCGC 2 cut(s) 2438, 4658
Hin1I GRCGYC 1 cut(s) 17
Hin6I GCGC 2 cut(s) 2436, 4656
HinP1I GCGC 2 cut(s) 2436, 4656
HindIII AAGCTT 3 cut(s) 134, 1781, 3971
HpaII CCGG 9 cut(s) 873, 907, 1603, 1709, 2113, 2849, 3094, 3793, 4198
Hpy99I CGWCG 4 cut(s) 19, 25, 80, 83
HpyCH4IV ACGT 4 cut(s) 763, 1594, 2953, 4242
HpySE526I ACGT 4 cut(s) 763, 1594, 2953, 4242
Hsp92I GRCGYC 1 cut(s) 17
HspAI GCGC 2 cut(s) 2436, 4656
Kpn2I TCCGGA 2 cut(s) 2112, 4197
KpnI GGTACC 1 cut(s) 2844
Ksp22I TGATCA 2 cut(s) 2218, 4303
KspI CCGCGG 1 cut(s) 577
Kzo9I GATC 9 cut(s) 886, 1335, 1361, 2115, 2218, 3073, 3319, 3551, 4303
LguI GCTCTTC 1 cut(s) 457
MaeI CTAG 9 cut(s) 167, 318, 387, 896, 1230, 2819, 3420, 3621, 4647
MaeII ACGT 4 cut(s) 763, 1594, 2953, 4242
MalI GATC 9 cut(s) 888, 1337, 1363, 2117, 2220, 3075, 3321, 3553, 4305
MbiI CCGCTC 1 cut(s) 467
MboI GATC 9 cut(s) 886, 1335, 1361, 2115, 2218, 3073, 3319, 3551, 4303
MfeI CAATTG 1 cut(s) 3033
MflI RGATCY 1 cut(s) 2115
MlyI GAGTC 4 cut(s) 1970, 2075, 2594, 4195
MmeI TCCRAC 5 cut(s) 1252, 1539, 2468, 3442, 4553
Mph1103I ATGCAT 5 cut(s) 1052, 2175, 3245, 3541, 4260
MroI TCCGGA 2 cut(s) 2112, 4197
MroXI GAANNNNTTC 2 cut(s) 2430, 4515
MseI TTAA 8 cut(s) 1164, 1214, 1809, 3357, 3404, 3560, 3606, 4667
MspI CCGG 9 cut(s) 873, 907, 1603, 1709, 2113, 2849, 3094, 3793, 4198
MunI CAATTG 1 cut(s) 3033
Mva1269I GAATGC 1 cut(s) 3183
MvaI CCWGG 4 cut(s) 11, 145, 2120, 2799
MvnI CGCG 2 cut(s) 576, 2436
NciI CCSGG 6 cut(s) 873, 874, 908, 1603, 3095, 3793
NdeI CATATG 1 cut(s) 59
NdeII GATC 9 cut(s) 886, 1335, 1361, 2115, 2218, 3073, 3319, 3551, 4303
NmuCI GTSAC 8 cut(s) 764, 806, 1247, 1922, 2027, 2539, 3623, 4112
NsiI ATGCAT 5 cut(s) 1052, 2175, 3245, 3541, 4260
NspI RCATGY 9 cut(s) 1143, 2159, 2177, 2201, 2501, 3336, 4262, 4286, 4586
OliI CACNNNNGTG 3 cut(s) 1929, 2034, 4119
PaeI GCATGC 2 cut(s) 2501, 4586
PaqCI CACCTGC 3 cut(s) 712, 2793, 2902
PasI CCCWGGG 1 cut(s) 144
PciI ACATGT 2 cut(s) 1139, 3332
PciSI GCTCTTC 1 cut(s) 457
PctI GAATGC 1 cut(s) 3183
PdmI GAANNNNTTC 2 cut(s) 2430, 4515
PflFI GACNNNGTC 2 cut(s) 19, 4469
PflMI CCANNNNNTGG 2 cut(s) 1271, 3461
PfoI TCCNGGA 1 cut(s) 2118
PleI GAGTC 4 cut(s) 1969, 2074, 2593, 4195
PpsI GAGTC 4 cut(s) 1969, 2074, 2593, 4195
Ppu21I YACGTR 2 cut(s) 764, 2954
PscI ACATGT 2 cut(s) 1139, 3332
PshAI GACNNNNGTC 4 cut(s) 1716, 2291, 3906, 4376
PsiI TTATAA 2 cut(s) 3668, 3720
Psp124BI GAGCTC 3 cut(s) 780, 943, 2970
Psp6I CCWGG 4 cut(s) 9, 143, 2118, 2797
PspEI GGTNACC 2 cut(s) 806, 3964
PspFI CCCAGC 4 cut(s) 606, 1374, 3564, 3651
PspGI CCWGG 4 cut(s) 9, 143, 2118, 2797
PspPI GGNCC 9 cut(s) 33, 110, 1718, 1837, 1949, 2054, 3908, 4027, 4683
PstI CTGCAG 4 cut(s) 399, 839, 2329, 4414
PstNI CAGNNNCTG 3 cut(s) 1206, 3090, 3396
PsuI RGATCY 1 cut(s) 2115
PsyI GACNNNGTC 2 cut(s) 19, 4469
RsaI GTAC 8 cut(s) 74, 309, 420, 824, 2178, 2662, 2842, 3011
RsaNI GTAC 8 cut(s) 73, 308, 419, 823, 2177, 2661, 2841, 3010
SacI GAGCTC 3 cut(s) 780, 943, 2970
SacII CCGCGG 1 cut(s) 577
SapI GCTCTTC 1 cut(s) 457
SaqAI TTAA 8 cut(s) 1164, 1214, 1809, 3357, 3404, 3560, 3606, 4667
Sau3AI GATC 9 cut(s) 886, 1335, 1361, 2115, 2218, 3073, 3319, 3551, 4303
Sau96I GGNCC 9 cut(s) 33, 110, 1718, 1837, 1949, 2054, 3908, 4027, 4683
SbfI CCTGCAGG 2 cut(s) 2329, 4414
SchI GAGTC 4 cut(s) 1970, 2075, 2594, 4195
SdaI CCTGCAGG 2 cut(s) 2329, 4414
SfcI CTRYAG 8 cut(s) 395, 835, 1476, 1863, 1968, 2325, 4053, 4410
Sfr303I CCGCGG 1 cut(s) 577
SgrBI CCGCGG 1 cut(s) 577
SinI GGWCC 3 cut(s) 110, 1718, 3908
SmaI CCCGGG 1 cut(s) 874
SphI GCATGC 2 cut(s) 2501, 4586
Sse8387I CCTGCAGG 2 cut(s) 2329, 4414
SspI AATATT 1 cut(s) 1813
SspMI CTAG 9 cut(s) 167, 318, 387, 896, 1230, 2819, 3420, 3621, 4647
SstI GAGCTC 3 cut(s) 780, 943, 2970
StyI CCWWGG 4 cut(s) 270, 342, 1265, 3455
TaiI ACGT 4 cut(s) 766, 1597, 2956, 4245
TatI WGTACW 3 cut(s) 822, 2176, 3009
TauI GCSGC 5 cut(s) 52, 160, 576, 579, 3027
Tru1I TTAA 8 cut(s) 1164, 1214, 1809, 3357, 3404, 3560, 3606, 4667
Tru9I TTAA 8 cut(s) 1164, 1214, 1809, 3357, 3404, 3560, 3606, 4667
TseFI GTSAC 8 cut(s) 764, 806, 1247, 1922, 2027, 2539, 3623, 4112
Tsp45I GTSAC 8 cut(s) 764, 806, 1247, 1922, 2027, 2539, 3623, 4112
TspMI CCCGGG 1 cut(s) 872
Tth111I GACNNNGTC 2 cut(s) 19, 4469
Van91I CCANNNNNTGG 2 cut(s) 1271, 3461
VneI GTGCAC 1 cut(s) 2638
VpaK11BI GGWCC 3 cut(s) 110, 1718, 3908
XagI CCTNNNNNAGG 1 cut(s) 148
XapI RAATTY 9 cut(s) 66, 1278, 1583, 1616, 2165, 2267, 3806, 4250, 4352
XbaI TCTAGA 3 cut(s) 1229, 2818, 3419
XceI RCATGY 9 cut(s) 1143, 2159, 2177, 2201, 2501, 3336, 4262, 4286, 4586
XcmI CCANNNNNNNNNTGG 4 cut(s) 1389, 1502, 3579, 3692
XmaI CCCGGG 1 cut(s) 872
XmiI GTMKAC 3 cut(s) 1646, 2695, 3836
XmnI GAANNNNTTC 2 cut(s) 2430, 4515
XspI CTAG 9 cut(s) 167, 318, 387, 896, 1230, 2819, 3420, 3621, 4647
Zsp2I ATGCAT 5 cut(s) 1052, 2175, 3245, 3541, 4260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.