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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 16, 148 |
| AdeI | CACNNNGTG | 1 cut(s) | 41 |
| AoxI | GGCC | 1 cut(s) | 150 |
| AspS9I | GGNCC | 1 cut(s) | 150 |
| AsuHPI | GGTGA | 2 cut(s) | 67, 112 |
| BmgT120I | GGNCC | 1 cut(s) | 150 |
| BseRI | GAGGAG | 1 cut(s) | 147 |
| BshFI | GGCC | 1 cut(s) | 152 |
| BsnI | GGCC | 1 cut(s) | 152 |
| BspACI | CCGC | 2 cut(s) | 16, 148 |
| BspANI | GGCC | 1 cut(s) | 152 |
| Bst4CI | ACNGT | 1 cut(s) | 85 |
| BstC8I | GCNNGC | 1 cut(s) | 150 |
| BstDEI | CTNAG | 1 cut(s) | 156 |
| BsuRI | GGCC | 1 cut(s) | 152 |
| Cac8I | GCNNGC | 1 cut(s) | 150 |
| Cfr13I | GGNCC | 1 cut(s) | 150 |
| CviJI | RGCY | 1 cut(s) | 152 |
| CviKI_1 | RGCY | 1 cut(s) | 152 |
| DdeI | CTNAG | 1 cut(s) | 156 |
| DraIII | CACNNNGTG | 1 cut(s) | 41 |
| FauI | CCCGC | 1 cut(s) | 141 |
| HaeIII | GGCC | 1 cut(s) | 152 |
| HinfI | GANTC | 1 cut(s) | 110 |
| HphI | GGTGA | 2 cut(s) | 67, 112 |
| Hpy188I | TCNGA | 2 cut(s) | 48, 121 |
| HpyCH4III | ACNGT | 1 cut(s) | 85 |
| HpyCH4V | TGCA | 1 cut(s) | 4 |
| HpyF3I | CTNAG | 1 cut(s) | 156 |
| MboII | GAAGA | 1 cut(s) | 41 |
| MlyI | GAGTC | 1 cut(s) | 119 |
| MnlI | CCTC | 3 cut(s) | 61, 125, 163 |
| PleI | GAGTC | 1 cut(s) | 118 |
| PpsI | GAGTC | 1 cut(s) | 118 |
| PspPI | GGNCC | 1 cut(s) | 150 |
| Sau96I | GGNCC | 1 cut(s) | 150 |
| SchI | GAGTC | 1 cut(s) | 119 |
| SetI | ASST | 1 cut(s) | 57 |
| SgeI | CNNG | 3 cut(s) | 48, 137, 161 |
| SsiI | CCGC | 2 cut(s) | 16, 148 |
| TaaI | ACNGT | 1 cut(s) | 85 |
| TaqI | TCGA | 1 cut(s) | 113 |
| TspGWI | ACGGA | 2 cut(s) | 52, 144 |