FvH4_5g29990

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000280
Physical Location & Seq
Forward (+)
20860033 .. 20865976
5944 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29990.t2

Sequence Viewer

Length: 2631 bp
ATGGTGGGGAGAAAGAGAGGTAGGGGCGGAGGCCGAGGCGGCAAGCGGCCGAGGAAAGAAGAGAACGGCGGCGAGGGTGTTTTGGGAATTGAGAATGAGGAAGTGAAACAAGTAGCTGTGGAGGAGAAGCCGAAGCCGGGCAGAGGCAGGAAGCGGCGGGGGCAGAAAGAAGAGGAAGAAGACTGCGGCGGAGGACAGAGGCGTTATACACTGAGGGAGAGGGCGTTGAAGGCGGAGACTCGGAAGAAGAGGCAGAGAAAGGTAGCGGCGGCGGCGAAGGAGGAGAGGCCTGACGTGTCGACTATGTGCCATCAGTGTCAGAGGAATGATAAGGGGGAAGTGGTGCGGTGCACGGGTTGTTGGAAAAGAAGAGAGAGGAAGAGATATTGTCTCCATTGTATTCAGACTTGGTATCCCAACGCCTCGGTGAAATCATTTGCTGAGGCTTGCCCCGTGTGCCGTGGCAACTGCAACTGCAAATCCTGCCTTCGTTTGGATGTGCCTGTCAGGTGTTTGGACAATGTGGACCCGAAAATCGATGATGAGGAGACTAAGTTGGAGCACTGTAAGTATTTGGTTCATAGCCTGCTTCCTTATTTGAAGAGGATCAAAGATGAGCAGGTCAGTGAGATGGTTGTGGAAGCCAAGAGGCAAGGCTTATCTCATGTTTCGCAGCTCCAAGTTCAAAAGTCTAATTGCCTTGAGGATGAGCGTATGTTTTGCAATCACTGCAATACTTCGATTTTCGATTTTCATAGGACCTGTCCTGACTGTGAGTATGATCTGTGCCTTGTTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAAGAAGAGAGAGGGGGAAGCGGGGCATAAGTATGTTGATTTGGGGCGTGAGTATTTGCATGGAGAGGGAGAGTTCAACAGCTTGCTAAAAAAGAACAGAGAAAATGTCAGAACGGGAAGAAGAGAACAGAAGAAGCGGAATGTTGATGTGGAAGTGAAGCCGGACAAGTTAGAATCTGCTGAAGAGGAAGATGAGAAATTTGTCAACTTGGGGGAGCATTTGGATGGAGAGGGAGAGCTGAAGAGGAATGATGATGTGGGAGTGGAGCAGGACGATTCTGAATCTGCTGAAGTGGCCACATTTGAGTGGAGAGCAAGTGAAGATGGAAGCATCCTCTGCCCCCCAAAGCACATGAAAGGTTGTGGTAAGCATACTTTAGAATTGAGATGCAATTTTTCTCAGAATCACGTCATAGAGTTGGTGGAGAAAGCAGGAAAAATAGATGAAAGTTACAATCTTTTGCATGCTAGTAGTGAGACTCCTGCACAGAAGTGTTCATGTGTTAAACCTGTGGATGATGCTGTTAGCATAAGTAGCAGTAGCTGTAAGATAAGGAAAGCAGCATATAGAGATGGTTCTGATGATAACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAAGATGAGGATTTTGGCCATTTTCAGTGGCACTGGATGAGGGGTGAGCCTGTAATTGTGAGTAATGCGCTTGAAAATGGATCAGGTCTAAGCTGGGAACCATCTGTCATGTGGCGGGCATTTAGACAGATAAAGAATCTCAACCATGATAGGGAATTGGCAGTCGATGCCATTGATTGCTTGGATTGGAATTTCATCGAAATCAACAACTGCGACTTCTTTACTGGATATTCCAAAGGCCGTTTTGATAAATTGGATTGGCCTGAGATTCTGAAGCTCAAAGACTTTCCTCCATCTACTGAGTTTGACAATCGTCTTCCTCGGCATGGTAAGGAATTTCTATGTTGTCTGCCTTTCAAGGAGTACACGCACCCCAAGGGAAGTCTGAACCTTGCTATGTTCTTGCCAAAGGAGTCAGTAAAGCCGGATATGGGTCCGAAAACATATATTGCCTATGGTGTTGTGCAGGAGCTTGGACGGGGAGATTCTGTAACCAAGCTTCACTGTGATATGTCTGATGCGGTCAATATTTTGACACATATAGCTGAAGTGACCCTTGCTCCTGAAAAACTTGCAACTATAGATGAGTTGAAGAAAAAACACAGGGAGCAAGACCGAAGGGAGATGTTTGGAAGTTGTGGCACAATTGCTGAAAATGGTAGTGTGCCTCAGGAAGATTCAGCTGAAGTATACGAAGATGGGGGTGCATTATGGGACATTTTCCGATGCCAGGATGTTCCACAGCTGGAGAAGTACATCCAGAAGCACATTAATGAATTCAGGCACTTGCACTGTAATCCATTGAAGAGTGTTACTCATGCTATACATGATCAGACTGTATATCTCACTGCGGAGCACAAGAGGAAGCTCAAGGAGGAATTCGGAATTCAGCCATGGACATTTGTCCAGAAACTTGGCGACGCTGTTTTCATTCCTGCAGGCTGTCCTCATCAGGTGAGGAATTTGAAGTCATGTATCAAAGTTGCACTGGACTTCGTTTCCCCTGAGAATGTGGGGGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACCCTTCCAACAAACCACAGGGCTAGTGAGGATAAGCTGGAGGTTAAGAAAATGATTGTGCATACTCTTTGTAATGCTGTGATGGAGATTACTGGTGAGAAAAAGTCTGAGGATGATGTGAAGCCTAAGAAGAGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

877

Amino Acids

100.45

Weight (kDa)

7.0

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjC PF02373 721 - 813 7.1e-13 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 610
AccB7I CCANNNNNTGG 1 cut(s) 1433
AccI GTMKAC 2 cut(s) 299, 2133
AclWI GGATC 2 cut(s) 614, 1531
AcoI YGGCCR 3 cut(s) 47, 1119, 1459
AcsI RAATTY 7 cut(s) 1022, 1633, 1778, 2219, 2321, 2328, 2404
AcuI CTGAAG 6 cut(s) 1026, 1085, 1134, 1736, 2010, 2148
AfaI GTAC 2 cut(s) 1808, 2198
AfiI CCNNNNNNNGG 9 cut(s) 137, 143, 493, 1433, 1450, 1594, 1769, 1874, 2173
AflIII ACRYGT 1 cut(s) 294
AgsI TTSAA 9 cut(s) 229, 601, 686, 901, 1517, 1801, 2035, 2248, 2410
AjiI CACGTC 2 cut(s) 295, 1234
AjnI CCWGG 1 cut(s) 2172
AleI CACNNNNGTG 1 cut(s) 1315
Alw21I GWGCWC 3 cut(s) 353, 564, 2301
Alw26I GTCTC 4 cut(s) 230, 395, 542, 1295
Alw44I GTGCAC 1 cut(s) 349
AlwI GGATC 2 cut(s) 614, 1531
AlwNI CAGNNNCTG 1 cut(s) 1368
AoxI GGCC 7 cut(s) 31, 47, 287, 1119, 1459, 1681, 1703
ApaLI GTGCAC 1 cut(s) 349
ApeKI GCWGC 3 cut(s) 673, 798, 1385
ApoI RAATTY 7 cut(s) 1022, 1633, 1778, 2219, 2321, 2328, 2404
AseI ATTAAT 1 cut(s) 2214
Asp700I GAANNNNTTC 1 cut(s) 2484
AspLEI GCGC 1 cut(s) 1513
AspS9I GGNCC 3 cut(s) 526, 759, 1877
AsuC2I CCSGG 2 cut(s) 138, 803
AsuHPI GGTGA 4 cut(s) 439, 1499, 2410, 2597
AvaII GGWCC 3 cut(s) 526, 759, 1877
AxyI CCTNAGG 1 cut(s) 2112
BaeGI GKGCMC 1 cut(s) 353
BalI TGGCCA 2 cut(s) 1121, 1461
BarI GAAGNNNNNNTAC 2 cut(s) 1926, 1958
BbsI GAAGAC 2 cut(s) 186, 1751
Bbv12I GWGCWC 3 cut(s) 353, 564, 2301
BbvCI CCTCAGC 1 cut(s) 441
BbvI GCAGC 3 cut(s) 685, 785, 1397
BceAI ACGGC 3 cut(s) 82, 444, 1668
BciT130I CCWGG 1 cut(s) 2174
BciVI GTATCC 1 cut(s) 423
BclI TGATCA 1 cut(s) 2272
BcnI CCSGG 2 cut(s) 138, 803
BcoDI GTCTC 4 cut(s) 230, 395, 542, 1295
BfaI CTAG 2 cut(s) 1293, 2514
BfmI CTRYAG 2 cut(s) 2022, 2379
BfuAI ACCTGC 1 cut(s) 610
BfuI GTATCC 1 cut(s) 423
BglI GCCNNNNNGGC 1 cut(s) 39
Bme1390I CCNGG 3 cut(s) 138, 803, 2174
Bme18I GGWCC 3 cut(s) 526, 759, 1877
BmgBI CACGTC 2 cut(s) 295, 1234
BmgT120I GGNCC 3 cut(s) 526, 759, 1877
BmiI GGNNCC 3 cut(s) 528, 1542, 1878
BmrFI CCNGG 3 cut(s) 138, 803, 2174
BmsI GCATC 6 cut(s) 1164, 1202, 1333, 1600, 1951, 2159
BoxI GACNNNNGTC 2 cut(s) 1755, 2345
BpiI GAAGAC 2 cut(s) 186, 1751
BplI GAGNNNNNCTC 2 cut(s) 2242, 2274
BpmI CTGGAG 2 cut(s) 2210, 2549
Bpu10I CCTNAGC 1 cut(s) 441
BpuEI CTTGAG 2 cut(s) 722, 2297
BpuMI CCSGG 2 cut(s) 138, 803
Bsa29I ATCGAT 1 cut(s) 537
BsaJI CCNNGG 8 cut(s) 34, 50, 423, 460, 1427, 1763, 1818, 2336
Bsc4I CCNNNNNNNGG 9 cut(s) 137, 143, 493, 1433, 1450, 1594, 1769, 1874, 2173
Bse1I ACTGG 4 cut(s) 1481, 1672, 2436, 2587
Bse21I CCTNAGG 1 cut(s) 2112
BseBI CCWGG 1 cut(s) 2174
BseCI ATCGAT 1 cut(s) 537
BseDI CCNNGG 8 cut(s) 34, 50, 423, 460, 1427, 1763, 1818, 2336
BseGI GGATG 9 cut(s) 502, 712, 1054, 1155, 1345, 1485, 2182, 2199, 2608
BseLI CCNNNNNNNGG 9 cut(s) 137, 143, 493, 1433, 1450, 1594, 1769, 1874, 2173
BseMII CTCAG 8 cut(s) 203, 432, 1238, 1698, 1734, 2126, 2439, 2589
BseNI ACTGG 4 cut(s) 1481, 1672, 2436, 2587
BseRI GAGGAG 3 cut(s) 137, 296, 560
BseSI GKGCMC 1 cut(s) 353
BseX3I CGGCCG 1 cut(s) 47
BseXI GCAGC 3 cut(s) 685, 785, 1397
BseYI CCCAGC 1 cut(s) 1536
BsgI GTGCAG 2 cut(s) 1293, 1928
Bsh1285I CGRYCG 1 cut(s) 50
BshFI GGCC 7 cut(s) 33, 49, 289, 1121, 1461, 1683, 1705
BshVI ATCGAT 1 cut(s) 537
BsiEI CGRYCG 1 cut(s) 50
BsiHKAI GWGCWC 3 cut(s) 353, 564, 2301
BsiSI CCGG 4 cut(s) 137, 802, 986, 1868
BslFI GGGAC 1 cut(s) 2171
BslI CCNNNNNNNGG 9 cut(s) 137, 143, 493, 1433, 1450, 1594, 1769, 1874, 2173
BsmAI GTCTC 4 cut(s) 230, 395, 542, 1295
BsmFI GGGAC 1 cut(s) 2171
BsnI GGCC 7 cut(s) 33, 49, 289, 1121, 1461, 1683, 1705
Bsp1286I GDGCHC 3 cut(s) 353, 564, 2301
Bsp143I GATC 4 cut(s) 606, 781, 1523, 2272
Bsp19I CCATGG 1 cut(s) 2336
BspANI GGCC 7 cut(s) 33, 49, 289, 1121, 1461, 1683, 1705
BspCNI CTCAG 8 cut(s) 204, 433, 1237, 1699, 1735, 2125, 2440, 2590
BspDI ATCGAT 1 cut(s) 537
BspLI GGNNCC 3 cut(s) 528, 1542, 1878
BspMAI CTGCAG 1 cut(s) 2383
BspMI ACCTGC 1 cut(s) 610
BspPI GGATC 2 cut(s) 614, 1531
BsrI ACTGG 4 cut(s) 1481, 1672, 2436, 2587
BssECI CCNNGG 8 cut(s) 34, 50, 423, 460, 1427, 1763, 1818, 2336
BssMI GATC 4 cut(s) 606, 781, 1523, 2272
BssNAI GTATAC 1 cut(s) 2134
BssT1I CCWWGG 3 cut(s) 1427, 1818, 2336
Bst1107I GTATAC 1 cut(s) 2134
Bst2UI CCWGG 1 cut(s) 2174
Bst4CI ACNGT 5 cut(s) 566, 773, 1949, 2237, 2281
BstAPI GCANNNNNTGC 4 cut(s) 483, 729, 1161, 1610
BstC8I GCNNGC 7 cut(s) 44, 448, 587, 908, 1290, 1560, 2383
BstDSI CCRYGG 2 cut(s) 460, 2336
BstF5I GGATG 9 cut(s) 502, 712, 1054, 1155, 1345, 1485, 2182, 2199, 2608
BstHHI GCGC 1 cut(s) 1513
BstKTI GATC 4 cut(s) 609, 784, 1526, 2275
BstMAI GTCTC 4 cut(s) 230, 395, 542, 1295
BstMBI GATC 4 cut(s) 606, 781, 1523, 2272
BstMCI CGRYCG 1 cut(s) 50
BstNI CCWGG 1 cut(s) 2174
BstNSI RCATGY 1 cut(s) 1292
BstPAI GACNNNNGTC 2 cut(s) 1755, 2345
BstSCI CCNGG 3 cut(s) 136, 801, 2172
BstSFI CTRYAG 2 cut(s) 2022, 2379
BstSLI GKGCMC 1 cut(s) 353
BstV1I GCAGC 3 cut(s) 685, 785, 1397
BstV2I GAAGAC 2 cut(s) 186, 1751
BstXI CCANNNNNNTGG 1 cut(s) 2357
BstZ17I GTATAC 1 cut(s) 2134
BstZI CGGCCG 1 cut(s) 47
Bsu15I ATCGAT 1 cut(s) 537
Bsu36I CCTNAGG 1 cut(s) 2112
BsuI GTATCC 1 cut(s) 423
BsuRI GGCC 7 cut(s) 33, 49, 289, 1121, 1461, 1683, 1705
BsuTUI ATCGAT 1 cut(s) 537
BtgI CCRYGG 2 cut(s) 460, 2336
BtrI CACGTC 2 cut(s) 295, 1234
BtsCI GGATG 9 cut(s) 502, 712, 1054, 1155, 1345, 1485, 2182, 2199, 2608
BtsI GCAGTG 2 cut(s) 727, 2289
BveI ACCTGC 1 cut(s) 610
Cac8I GCNNGC 7 cut(s) 44, 448, 587, 908, 1290, 1560, 2383
CaiI CAGNNNCTG 1 cut(s) 1368
CfoI GCGC 1 cut(s) 1513
Cfr13I GGNCC 3 cut(s) 526, 759, 1877
ClaI ATCGAT 1 cut(s) 537
CseI GACGC 1 cut(s) 2372
Csp6I GTAC 2 cut(s) 1807, 2197
CviQI GTAC 2 cut(s) 1807, 2197
DpnI GATC 4 cut(s) 608, 783, 1525, 2274
DpnII GATC 4 cut(s) 606, 781, 1523, 2272
EaeI YGGCCR 3 cut(s) 47, 1119, 1459
EagI CGGCCG 1 cut(s) 47
EciI GGCGGA 3 cut(s) 42, 204, 248
EclXI CGGCCG 1 cut(s) 47
Eco130I CCWWGG 3 cut(s) 1427, 1818, 2336
Eco147I AGGCCT 1 cut(s) 289
Eco47I GGWCC 3 cut(s) 526, 759, 1877
Eco52I CGGCCG 1 cut(s) 47
Eco57I CTGAAG 6 cut(s) 1026, 1085, 1134, 1736, 2010, 2148
Eco81I CCTNAGG 1 cut(s) 2112
EcoO109I RGGNCCY 1 cut(s) 759
EcoRI GAATTC 3 cut(s) 2219, 2321, 2328
EcoRII CCWGG 1 cut(s) 2172
EcoT14I CCWWGG 3 cut(s) 1427, 1818, 2336
ErhI CCWWGG 3 cut(s) 1427, 1818, 2336
FalI AAGNNNNNCTT 2 cut(s) 1983, 2015
FaqI GGGAC 1 cut(s) 2171
FauI CCCGC 3 cut(s) 150, 838, 1551
FbaI TGATCA 1 cut(s) 2272
FblI GTMKAC 2 cut(s) 299, 2133
FokI GGATG 9 cut(s) 509, 719, 1061, 1142, 1352, 1492, 2186, 2189, 2615
FspBI CTAG 2 cut(s) 1293, 2514
GlaI GCGC 1 cut(s) 1512
GsaI CCCAGC 1 cut(s) 1540
GsuI CTGGAG 2 cut(s) 2210, 2549
HaeIII GGCC 7 cut(s) 33, 49, 289, 1121, 1461, 1683, 1705
HapII CCGG 4 cut(s) 137, 802, 986, 1868
HgaI GACGC 1 cut(s) 2372
HhaI GCGC 1 cut(s) 1513
Hin6I GCGC 1 cut(s) 1511
HinP1I GCGC 1 cut(s) 1511
HincII GTYRAC 2 cut(s) 300, 1030
HindII GTYRAC 2 cut(s) 300, 1030
HindIII AAGCTT 1 cut(s) 1940
HpaII CCGG 4 cut(s) 137, 802, 986, 1868
HphI GGTGA 4 cut(s) 439, 1499, 2410, 2597
Hpy166II GTNNAC 6 cut(s) 300, 351, 526, 1030, 1809, 2134
Hpy188III TCNNGA 6 cut(s) 767, 812, 2006, 2114, 2203, 2350
Hpy8I GTNNAC 6 cut(s) 300, 351, 526, 1030, 1809, 2134
Hpy99I CGWCG 1 cut(s) 2366
HpyAV CCTTC 6 cut(s) 223, 271, 497, 2055, 2504, 2619
HpyCH4III ACNGT 5 cut(s) 566, 773, 1949, 2237, 2281
HpyCH4IV ACGT 2 cut(s) 294, 1233
HpySE526I ACGT 2 cut(s) 294, 1233
HspAI GCGC 1 cut(s) 1511
Ksp22I TGATCA 1 cut(s) 2272
Kzo9I GATC 4 cut(s) 606, 781, 1523, 2272
LmnI GCTCC 8 cut(s) 559, 681, 1039, 1090, 1912, 2008, 2050, 2296
Lsp1109I GCAGC 3 cut(s) 685, 785, 1397
LweI GCATC 6 cut(s) 1164, 1202, 1333, 1600, 1951, 2159
MaeI CTAG 2 cut(s) 1293, 2514
MaeII ACGT 2 cut(s) 294, 1233
MaeIII GTNAC 4 cut(s) 1274, 1933, 1993, 2254
MalI GATC 4 cut(s) 608, 783, 1525, 2274
MboI GATC 4 cut(s) 606, 781, 1523, 2272
MfeI CAATTG 1 cut(s) 2088
MhlI GDGCHC 3 cut(s) 353, 564, 2301
MlsI TGGCCA 2 cut(s) 1121, 1461
MluNI TGGCCA 2 cut(s) 1121, 1461
MlyI GAGTC 3 cut(s) 232, 1297, 1865
MmeI TCCRAC 4 cut(s) 341, 537, 1414, 2522
Mox20I TGGCCA 2 cut(s) 1121, 1461
MroXI GAANNNNTTC 1 cut(s) 2484
MscI TGGCCA 2 cut(s) 1121, 1461
MseI TTAA 3 cut(s) 1329, 2214, 2535
MslI CAYNNNNRTG 5 cut(s) 855, 1047, 1129, 1315, 2214
Msp20I TGGCCA 2 cut(s) 1121, 1461
MspA1I CMGCKG 2 cut(s) 2126, 2188
MspI CCGG 4 cut(s) 137, 802, 986, 1868
MspR9I CCNGG 3 cut(s) 138, 803, 2174
MunI CAATTG 1 cut(s) 2088
MvaI CCWGG 1 cut(s) 2174
NciI CCSGG 2 cut(s) 138, 803
NcoI CCATGG 1 cut(s) 2336
NdeII GATC 4 cut(s) 606, 781, 1523, 2272
NlaIV GGNNCC 3 cut(s) 528, 1542, 1878
NmeAIII GCCGAG 3 cut(s) 59, 75, 1744
NmuCI GTSAC 1 cut(s) 1993
NspI RCATGY 1 cut(s) 1292
OliI CACNNNNGTG 1 cut(s) 1315
PaeI GCATGC 1 cut(s) 1292
PceI AGGCCT 1 cut(s) 289
PdmI GAANNNNTTC 1 cut(s) 2484
PfeI GAWTC 9 cut(s) 808, 998, 1100, 1106, 1228, 1579, 1711, 1928, 2120
PflMI CCANNNNNTGG 1 cut(s) 1433
PleI GAGTC 3 cut(s) 232, 1297, 1864
PpsI GAGTC 3 cut(s) 232, 1297, 1864
PpuMI RGGWCCY 1 cut(s) 759
PshAI GACNNNNGTC 2 cut(s) 1755, 2345
PshBI ATTAAT 1 cut(s) 2214
Psp5II RGGWCCY 1 cut(s) 759
Psp6I CCWGG 1 cut(s) 2172
PspFI CCCAGC 1 cut(s) 1536
PspGI CCWGG 1 cut(s) 2172
PspN4I GGNNCC 3 cut(s) 528, 1542, 1878
PspPI GGNCC 3 cut(s) 526, 759, 1877
PspPPI RGGWCCY 1 cut(s) 759
PstI CTGCAG 1 cut(s) 2383
PstNI CAGNNNCTG 1 cut(s) 1368
PvuII CAGCTG 2 cut(s) 2126, 2188
RsaI GTAC 2 cut(s) 1808, 2198
RsaNI GTAC 2 cut(s) 1807, 2197
RseI CAYNNNNRTG 5 cut(s) 855, 1047, 1129, 1315, 2214
SalI GTCGAC 1 cut(s) 298
SaqAI TTAA 3 cut(s) 1329, 2214, 2535
Sau3AI GATC 4 cut(s) 606, 781, 1523, 2272
Sau96I GGNCC 3 cut(s) 526, 759, 1877
SbfI CCTGCAGG 1 cut(s) 2383
SchI GAGTC 3 cut(s) 232, 1297, 1865
ScrFI CCNGG 3 cut(s) 138, 803, 2174
SdaI CCTGCAGG 1 cut(s) 2383
SduI GDGCHC 3 cut(s) 353, 564, 2301
SfaNI GCATC 6 cut(s) 1164, 1202, 1333, 1600, 1951, 2159
SfcI CTRYAG 2 cut(s) 2022, 2379
SinI GGWCC 3 cut(s) 526, 759, 1877
SmiMI CAYNNNNRTG 5 cut(s) 855, 1047, 1129, 1315, 2214
SmlI CTYRAG 2 cut(s) 701, 2312
SmoI CTYRAG 2 cut(s) 701, 2312
SphI GCATGC 1 cut(s) 1292
Sse8387I CCTGCAGG 1 cut(s) 2383
SseBI AGGCCT 1 cut(s) 289
SspI AATATT 1 cut(s) 1972
SspMI CTAG 2 cut(s) 1293, 2514
StuI AGGCCT 1 cut(s) 289
StyD4I CCNGG 3 cut(s) 136, 801, 2172
StyI CCWWGG 3 cut(s) 1427, 1818, 2336
TaaI ACNGT 5 cut(s) 566, 773, 1949, 2237, 2281
TaiI ACGT 2 cut(s) 297, 1236
TaqI TCGA 7 cut(s) 299, 537, 740, 747, 811, 1608, 1641
TaqII GACCGA 1 cut(s) 2073
TatI WGTACW 2 cut(s) 1806, 2196
TauI GCSGC 8 cut(s) 42, 49, 72, 157, 189, 269, 272, 275
TfiI GAWTC 9 cut(s) 808, 998, 1100, 1106, 1228, 1579, 1711, 1928, 2120
Tru1I TTAA 3 cut(s) 1329, 2214, 2535
Tru9I TTAA 3 cut(s) 1329, 2214, 2535
TseFI GTSAC 1 cut(s) 1993
TseI GCWGC 3 cut(s) 673, 798, 1385
Tsp45I GTSAC 1 cut(s) 1993
TspDTI ATGAA 8 cut(s) 569, 743, 1193, 1284, 1311, 1627, 2232, 2362
TspGWI ACGGA 1 cut(s) 2492
Van91I CCANNNNNTGG 1 cut(s) 1433
VneI GTGCAC 1 cut(s) 349
VpaK11BI GGWCC 3 cut(s) 526, 759, 1877
VspI ATTAAT 1 cut(s) 2214
XapI RAATTY 7 cut(s) 1022, 1633, 1778, 2219, 2321, 2328, 2404
XceI RCATGY 1 cut(s) 1292
XcmI CCANNNNNNNNNTGG 2 cut(s) 1551, 1621
XmiI GTMKAC 2 cut(s) 299, 2133
XmnI GAANNNNTTC 1 cut(s) 2484
XspI CTAG 2 cut(s) 1293, 2514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.