Rh7BG356400

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000280
Physical Location & Seq
Forward (+)
37430990 .. 37435906
4917 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG356400.1

Sequence Viewer

Length: 4533 bp
ATGAAATCCAAGGCGACGGCGACGAGATTGAGGCCCGGAGGCACAAAGCGGCGGAACGGCGGTGGTGTGGGTACTTTGGGAATTCAGAACGACGACGGAACAAATGGTGGAGGACCAAAGCTTGTTCCCTGGGGGAGGAAGCGGCGGAGGACAGGATGCGAGGGTATTTTGGGATATCAAAATGAAGGAGAGGCAAGGGGGGCGAAGAAGAAGAAGAAGAAGAAGCAGCAGCAGCAGAGAAGGGCTCCCTTGAAGGAGGTGACAGCGTGCCATCAATGTAGGAAAACAGGTAAGGGAAGAATGGTGCGGTGCACATGGTGTTCGAGGAACAGGTATTGTATCCCTTGCATACAGAGGTGGTATCCCAACACATCAGAGGAAGCCATAGCTGAGGCATGTCCTGTGTGCCGCGGCAACTGCAACTGCTATACCTGCCTTCGATTGGATGTGCCTAATAGGTGTTTGAAGAATCTAGACCCGGAAATCGATCAGGAGACCAAGCTGGAGCACTGTAAGTATATGGTTCATCAGCTGCTTCCGTATTTGAAGAGGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTCGAGGCTAAGAGGCAGGGTTTAGGTGATGTTTCGGAGCTCAAGGTGGAGAAGGCTGATTGCGGTGAAGACGAGCGTATGTACTGCAACAACTGCAGCACTTCGATTTTTGATTTTCATAGGACTTGTCCTGAGTGTGAGTATGATCTGTGCCTCGGTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAACAAGAGAGCGGAAGTGTCCACATTTGAGTGGAGAACAAGTGAAGATGGAAGCATTCTCTGCCCCCCAACACACATGAAAGGTTGTGGTAAGCATAATTTAGAATTGAGATGCCTTTTTCCTCAGAATCATGTCATGGAATTGGTAGAGAATGCAGGAAGAATAGATGCAAGTTACAATTTTTTGCCTGATCGTAGTGAAACATGTGCATATAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTTGCTCAAGTAGCAGCAGCTCTAAGTTAAGGAAAGCAGCTTCTAGAGTTTCTGATGATAACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAGGATAAGGATTTTGAGCATTTTCAGTGGCATTGGAAGAAGGGTGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCGGGTTTAAGCTGGGAACCATCTGTCATGTGGCGGGCATTTAAGCAGACAAACTATATCAACCGTGCTAGTGAATCAACATTCCACGCCATTGATTGCTGGTATTGGTGTTTTATAGAAATCCACATCCACCATTTCTGTAGTGGATATTCCAAGGGACGGTCGTATAAGACAGGATGGCCCCAGAATCTCAAGCTCACTGACTTCCCATCATCTACTGAATTTCACAATCGTCTTCCCCGGCATGGTGAGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATACTCACCCCAACAAGGGAACTCTGAACCTTGCTATCTACTTGCCACCTATCTCAGTAAAGCCGGACATGGGTCCAAAAACGTATATTGCCTATGGTTTTGCAGAGGAGCTTGGATGGGGAGATTCTGTAACCAAGCTTCACTGCAATATGTCTGATGCAGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACAACTGAAGAACTTGCAACTATAGATAAGTTGAAGAAAAAGCTGAGGGAGCAAGACGAAAGGGAGATATTTGGGAATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCTCAGCAAGAGTCAGCTATAGAGAAGTTGAAGAGAAAGTGTAGGGAGCAAAACGAAAGAGAGATATTTGGGAATTGTCATACAAGTGCTGGAAATGGAAGTGGGCCTCAGCAAGAGTCAGCTGAAATATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGATCCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTATGGATGTGCTCTGTAATCCTTTGCAGCATGTTACTCATGCTATACACGATCAAACTGTCTATCTCACTGTGGAACACAAGAGGAAGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTGGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCAACAAACCACAGAGCCCATGAGGACAAGATGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTATGAAGGAGATTCCTGGTGAGAATGTGACAAAGAGGGCAAGGCCTGTGAAGGCAAAGAGGGCAAGGGTTCGCTTGGACGAGTTGAGACGTTGCCATCAGTGTCATATGAATGTTATGGGAAGAGTGGTGCAGTGCACAAGGTGTCCGGAAAGAGGTACGGGAAGGAGGACAAGGTATTGTATCCCTTGTATACAGAGATGGTATCCCAACACATCAGAGGAAGCCATTGCTAAGGCTTGTCCTTTATGCTGCGGCAACTGCAGCTGCACAGCCTGTCTTCGCTTGGATGTGCCTGTCAGGAGTTTGCAGAATCTAGAGCCAAAAATCGACAAGGGTACCAAGCTGGAGCACTCTAAGTATTTGGTTCATCAGCTGCTTCCCTATTTGAAGATGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTGGAAGCCAAGAGGCAGGGCTTACGTGACCTTTCGGAGCTCAAGGTGGAGAAGGCTAAAAGCGGTGACGGAGTGCGTATGTACTGCAACAACTGCAGCACTTCAATTTTTGATTTTCATAGGACTTGTCCCGGATGTGAGTATGATCTGTGCCTCAGTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAAGAAGAGAGCTCAAGCGTCCACATTTGAGTGGAAATGGAGAGCAAGTGAAGATGGAAGCATTCTCTGCCCCCCAACACACAGTTGTGGTAAGCACAATTTAGAATTGAGATGCATTTTCTCTCAGAATCATGTCATGGAATTGGTAGAGAAAGCAGGAGAAATAGATGCAAGTTACAATTTTTTGCCTGATCGTAGTGAAACATGTGCATATAAGTGTTCATGTGTTAAACCTGTGGATGATGCCATTAGCTTAAGTAGTAGCAGCTGCTGTAAGTTAAGGAAAGCAGCTTCTAGAGTTGCTTCTGATAGTAACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAAGATAAGGATTTTGAGCATTTTCAGTGGCATTGGAAGAGGGGGGAGCCTGTGATCATTAGCAATGCGCTTGAAAATGGATCAGGTTTAAGCTGGGAACCATATGTCATGTGGCAGGCATTTAGAAAGACAAATAATATCAACCATGCTAGTGAATCGACAGTCCATGCCATTGATTGCTGGGATTGGTGTTGTATAGAAATCAACATCCACCATTTCTTTAGTGGATATTCCAAAGGACGGTCTTATAAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATCATCTACTGAGTTTCACAATCGTCTTCCCCAGCATGGTAGGGAATTTTTATGTTGCCTGCCTTTCAAGGAGTATACTCACCCCAACAAGGGAACTCTGAACCTTGCTAACAACTTGCCACCTATCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACGGGGAGATTCTGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACAACTGAACAACTTGTAACTGTAGAGAAGTTGAAGAAAAAGCACAGGGAGCAAGACAAAAGAGAGATATTTGGGAATTGTCACTCAAGTGCTGGAAATGGAAGTGGGTCTCAGGAAGAATTAGCTGATGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGACTCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTAAGCATGTGTTCTGTAATCCTTTGCAGCATGTTACTCATGCTATACATGATCAGACTGTTTATCTCACTGTGGAGCACAAGAGGAAGCTGAAGGAGGAATTCGGTATCGAGCCTTGGACATTTGTCCAAAAACCTGTTGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTGGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACATACCACAGAGCCCATGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTGTGAAGGAGATTTCTGGTGAGAATGTGATGACTGAGGATGGGAAGTCTAGGGGAGGCGCAAGGGAATTAAGGCAAGGTGTAAGGCGCAAGTCTTACAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1510

Amino Acids

172.33

Weight (kDa)

8.45

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 86 - 147 1.7e-06 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 651 - 738 5.4e-13 JmjC domain, hydroxylase
JmjC PF02373 1336 - 1433 1.9e-12 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3549
AarI CACCTGC 2 cut(s) 556, 2740
Acc36I ACCTGC 5 cut(s) 440, 556, 2147, 2740, 4232
Acc65I GGTACC 1 cut(s) 2678
AccB1I GGYRCC 3 cut(s) 1922, 2678, 4007
AccB7I CCANNNNNTGG 2 cut(s) 1100, 3290
AccBSI CCGCTC 1 cut(s) 782
AccI GTMKAC 3 cut(s) 1475, 2533, 3665
AccII CGCG 1 cut(s) 411
AccIII TCCGGA 3 cut(s) 1941, 2488, 4026
AclWI GGATC 4 cut(s) 1198, 1939, 1952, 3388
AcsI RAATTY 8 cut(s) 81, 1412, 1445, 1994, 2096, 3635, 4079, 4181
AcuI CTGAAG 4 cut(s) 1680, 1698, 3870, 4193
AdeI CACNNNGTG 2 cut(s) 318, 2484
AfaI GTAC 5 cut(s) 73, 665, 2500, 2680, 2849
AflII CTTAAG 1 cut(s) 3208
AflIII ACRYGT 2 cut(s) 974, 3158
AjiI CACGTC 2 cut(s) 1987, 4072
AjnI CCWGG 4 cut(s) 128, 1110, 1947, 2356
AleI CACNNNNGTG 3 cut(s) 1758, 3039, 3948
Alw21I GWGCWC 9 cut(s) 314, 510, 624, 2010, 2480, 2694, 2808, 2968, 4161
Alw26I GTCTC 3 cut(s) 488, 2422, 3975
Alw44I GTGCAC 2 cut(s) 310, 2476
AlwI GGATC 4 cut(s) 1198, 1939, 1952, 3388
AlwNI CAGNNNCTG 2 cut(s) 2928, 3225
Aor13HI TCCGGA 3 cut(s) 1941, 2488, 4026
AoxI GGCC 8 cut(s) 32, 1370, 1665, 1778, 1883, 2384, 3560, 3855
ApaLI GTGCAC 2 cut(s) 310, 2476
ApoI RAATTY 8 cut(s) 81, 1412, 1445, 1994, 2096, 3635, 4079, 4181
Asp700I GAANNNNTTC 2 cut(s) 2259, 4344
Asp718I GGTACC 1 cut(s) 2678
AspLEI GCGC 3 cut(s) 3370, 4487, 4515
AspS9I GGNCC 9 cut(s) 33, 113, 1371, 1547, 1666, 1778, 1883, 3737, 3856
AsuC2I CCSGG 6 cut(s) 36, 479, 749, 1432, 2898, 2933
AvaII GGWCC 3 cut(s) 113, 1547, 3737
BaeGI GKGCMC 2 cut(s) 314, 2480
BaeI ACNNNNGTAYC 2 cut(s) 2662, 2695
BamHI GGATCC 1 cut(s) 1944
BanI GGYRCC 3 cut(s) 1922, 2678, 4007
BanII GRGCYC 6 cut(s) 247, 624, 2290, 2808, 2968, 4375
BarI GAAGNNNNNNTAC 4 cut(s) 1596, 1628, 3786, 3818
BbsI GAAGAC 4 cut(s) 657, 1418, 2612, 3608
Bbv12I GWGCWC 9 cut(s) 314, 510, 624, 2010, 2480, 2694, 2808, 2968, 4161
BbvCI CCTCAGC 4 cut(s) 390, 1715, 1782, 1887
BceAI ACGGC 4 cut(s) 33, 73, 1001, 2095
BciT130I CCWGG 4 cut(s) 130, 1112, 1949, 2358
BciVI GTATCC 4 cut(s) 350, 372, 2534, 2556
BclI TGATCA 2 cut(s) 3354, 4132
BcnI CCSGG 6 cut(s) 36, 479, 749, 1432, 2898, 2933
BcoDI GTCTC 3 cut(s) 488, 2422, 3975
BfaI CTAG 7 cut(s) 473, 1062, 1260, 2657, 3249, 3450, 4476
BfmI CTRYAG 9 cut(s) 676, 1330, 1692, 1797, 2154, 2602, 2860, 3882, 4239
BfrI CTTAAG 1 cut(s) 3208
BfuAI ACCTGC 5 cut(s) 440, 556, 2147, 2740, 4232
BfuI GTATCC 4 cut(s) 350, 372, 2534, 2556
Bme18I GGWCC 3 cut(s) 113, 1547, 3737
BmgBI CACGTC 2 cut(s) 1987, 4072
BmgT120I GGNCC 9 cut(s) 33, 113, 1371, 1547, 1666, 1778, 1883, 3737, 3856
BoxI GACNNNNGTC 4 cut(s) 1545, 2120, 3735, 4205
BpiI GAAGAC 4 cut(s) 657, 1418, 2612, 3608
BplI GAGNNNNNCTC 2 cut(s) 229, 261
BpmI CTGGAG 5 cut(s) 524, 1985, 2708, 4070, 4409
Bpu10I CCTNAGC 5 cut(s) 390, 1715, 1782, 1887, 2574
BpuEI CTTGAG 8 cut(s) 608, 1009, 1367, 2072, 2792, 2952, 3557, 3931
BpuMI CCSGG 6 cut(s) 36, 479, 749, 1432, 2898, 2933
Bsa29I ATCGAT 1 cut(s) 486
BsaAI YACGTR 1 cut(s) 2792
BsaI GGTCTC 2 cut(s) 488, 3975
BsaWI WCCGGW 3 cut(s) 1941, 2488, 4026
Bse1I ACTGG 2 cut(s) 2211, 4296
Bse3DI GCAATG 3 cut(s) 1180, 2568, 3370
BseAI TCCGGA 3 cut(s) 1941, 2488, 4026
BseBI CCWGG 4 cut(s) 130, 1112, 1949, 2358
BseCI ATCGAT 1 cut(s) 486
BseMI GCAATG 3 cut(s) 1180, 2568, 3370
BseNI ACTGG 2 cut(s) 2211, 4296
BseRI GAGGAG 1 cut(s) 1595
BseSI GKGCMC 2 cut(s) 314, 2480
BseYI CCCAGC 4 cut(s) 1203, 3393, 3480, 3621
BsgI GTGCAG 2 cut(s) 2492, 2593
Bsh1236I CGCG 1 cut(s) 411
Bsh1285I CGRYCG 1 cut(s) 1355
BshFI GGCC 8 cut(s) 34, 1372, 1667, 1780, 1885, 2386, 3562, 3857
BshNI GGYRCC 3 cut(s) 1922, 2678, 4007
BshVI ATCGAT 1 cut(s) 486
BsiEI CGRYCG 1 cut(s) 1355
BsiHKAI GWGCWC 9 cut(s) 314, 510, 624, 2010, 2480, 2694, 2808, 2968, 4161
BslFI GGGAC 4 cut(s) 1362, 1946, 2880, 4031
BsmAI GTCTC 3 cut(s) 488, 2422, 3975
BsmBI CGTCTC 1 cut(s) 2422
BsmFI GGGAC 4 cut(s) 1362, 1946, 2880, 4031
BsmI GAATGC 3 cut(s) 825, 928, 3015
BsnI GGCC 8 cut(s) 34, 1372, 1667, 1780, 1885, 2386, 3562, 3857
Bso31I GGTCTC 2 cut(s) 488, 3975
Bsp13I TCCGGA 3 cut(s) 1941, 2488, 4026
BspANI GGCC 8 cut(s) 34, 1372, 1667, 1780, 1885, 2386, 3562, 3857
BspDI ATCGAT 1 cut(s) 486
BspEI TCCGGA 3 cut(s) 1941, 2488, 4026
BspFNI CGCG 1 cut(s) 411
BspMAI CTGCAG 5 cut(s) 680, 2158, 2606, 2864, 4243
BspMI ACCTGC 5 cut(s) 440, 556, 2147, 2740, 4232
BspPI GGATC 4 cut(s) 1198, 1939, 1952, 3388
BspT107I GGYRCC 3 cut(s) 1922, 2678, 4007
BspTI CTTAAG 1 cut(s) 3208
BspTNI GGTCTC 2 cut(s) 488, 3975
BsrBI CCGCTC 1 cut(s) 782
BsrDI GCAATG 3 cut(s) 1180, 2568, 3370
BsrI ACTGG 2 cut(s) 2211, 4296
BssNAI GTATAC 3 cut(s) 1476, 2534, 3666
BssT1I CCWWGG 5 cut(s) 9, 1094, 1344, 3284, 4196
Bst1107I GTATAC 3 cut(s) 1476, 2534, 3666
Bst2UI CCWGG 4 cut(s) 130, 1112, 1949, 2358
Bst6I CTCTTC 7 cut(s) 542, 1805, 2250, 2458, 2954, 3332, 4335
BstAFI CTTAAG 1 cut(s) 3208
BstAPI GCANNNNNTGC 4 cut(s) 675, 831, 2859, 3021
BstBAI YACGTR 1 cut(s) 2792
BstC8I GCNNGC 6 cut(s) 268, 1227, 2328, 3417, 3650, 4413
BstDSI CCRYGG 2 cut(s) 409, 2111
BstENI CCTNNNNNAGG 1 cut(s) 133
BstFNI CGCG 1 cut(s) 411
BstHHI GCGC 3 cut(s) 3370, 4487, 4515
BstMAI GTCTC 3 cut(s) 488, 2422, 3975
BstMCI CGRYCG 1 cut(s) 1355
BstNI CCWGG 4 cut(s) 130, 1112, 1949, 2358
BstNSI RCATGY 8 cut(s) 399, 978, 2030, 2330, 3162, 4091, 4115, 4415
BstPAI GACNNNNGTC 4 cut(s) 1545, 2120, 3735, 4205
BstSFI CTRYAG 9 cut(s) 676, 1330, 1692, 1797, 2154, 2602, 2860, 3882, 4239
BstSLI GKGCMC 2 cut(s) 314, 2480
BstUI CGCG 1 cut(s) 411
BstV2I GAAGAC 4 cut(s) 657, 1418, 2612, 3608
BstX2I RGATCY 1 cut(s) 1944
BstXI CCANNNNNNTGG 1 cut(s) 2132
BstYI RGATCY 1 cut(s) 1944
BstZ17I GTATAC 3 cut(s) 1476, 2534, 3666
Bsu15I ATCGAT 1 cut(s) 486
BsuI GTATCC 4 cut(s) 350, 372, 2534, 2556
BsuRI GGCC 8 cut(s) 34, 1372, 1667, 1780, 1885, 2386, 3562, 3857
BsuTUI ATCGAT 1 cut(s) 486
BtgI CCRYGG 2 cut(s) 409, 2111
BtrI CACGTC 2 cut(s) 1987, 4072
BtsI GCAGTG 3 cut(s) 1615, 2480, 3805
BveI ACCTGC 5 cut(s) 440, 556, 2147, 2740, 4232
Cac8I GCNNGC 6 cut(s) 268, 1227, 2328, 3417, 3650, 4413
CaiI CAGNNNCTG 2 cut(s) 2928, 3225
CfoI GCGC 3 cut(s) 3370, 4487, 4515
Cfr13I GGNCC 9 cut(s) 33, 113, 1371, 1547, 1666, 1778, 1883, 3737, 3856
Cfr42I CCGCGG 1 cut(s) 412
ClaI ATCGAT 1 cut(s) 486
CseI GACGC 1 cut(s) 2961
Csp6I GTAC 5 cut(s) 72, 664, 2499, 2679, 2848
CviQI GTAC 5 cut(s) 72, 664, 2499, 2679, 2848
DraIII CACNNNGTG 2 cut(s) 318, 2484
Eam1104I CTCTTC 7 cut(s) 542, 1805, 2250, 2458, 2954, 3332, 4335
EarI CTCTTC 7 cut(s) 542, 1805, 2250, 2458, 2954, 3332, 4335
EciI GGCGGA 2 cut(s) 67, 160
Ecl136II GAGCTC 3 cut(s) 622, 2806, 2966
Eco130I CCWWGG 5 cut(s) 9, 1094, 1344, 3284, 4196
Eco147I AGGCCT 1 cut(s) 2386
Eco24I GRGCYC 6 cut(s) 247, 624, 2290, 2808, 2968, 4375
Eco31I GGTCTC 2 cut(s) 488, 3975
Eco32I GATATC 1 cut(s) 176
Eco47I GGWCC 3 cut(s) 113, 1547, 3737
Eco53kI GAGCTC 3 cut(s) 622, 2806, 2966
Eco57I CTGAAG 4 cut(s) 1680, 1698, 3870, 4193
EcoICRI GAGCTC 3 cut(s) 622, 2806, 2966
EcoNI CCTNNNNNAGG 1 cut(s) 133
EcoRI GAATTC 3 cut(s) 81, 2096, 4181
EcoRII CCWGG 4 cut(s) 128, 1110, 1947, 2356
EcoRV GATATC 1 cut(s) 176
EcoT14I CCWWGG 5 cut(s) 9, 1094, 1344, 3284, 4196
EcoT22I ATGCAT 1 cut(s) 3071
EcoT38I GRGCYC 6 cut(s) 247, 624, 2290, 2808, 2968, 4375
ErhI CCWWGG 5 cut(s) 9, 1094, 1344, 3284, 4196
Esp3I CGTCTC 1 cut(s) 2422
FalI AAGNNNNNCTT 4 cut(s) 1653, 1685, 3843, 3875
FaqI GGGAC 4 cut(s) 1362, 1946, 2880, 4031
FauI CCCGC 1 cut(s) 1218
FauNDI CATATG 2 cut(s) 2448, 3403
FbaI TGATCA 2 cut(s) 3354, 4132
FblI GTMKAC 3 cut(s) 1475, 2533, 3665
FriOI GRGCYC 6 cut(s) 247, 624, 2290, 2808, 2968, 4375
FspBI CTAG 7 cut(s) 473, 1062, 1260, 2657, 3249, 3450, 4476
GlaI GCGC 3 cut(s) 3369, 4486, 4514
GsaI CCCAGC 4 cut(s) 1207, 3397, 3484, 3625
GsuI CTGGAG 5 cut(s) 524, 1985, 2708, 4070, 4409
HaeIII GGCC 8 cut(s) 34, 1372, 1667, 1780, 1885, 2386, 3562, 3857
HgaI GACGC 1 cut(s) 2961
HhaI GCGC 3 cut(s) 3370, 4487, 4515
Hin6I GCGC 3 cut(s) 3368, 4485, 4513
HinP1I GCGC 3 cut(s) 3368, 4485, 4513
HindIII AAGCTT 3 cut(s) 119, 1610, 3800
Hpy166II GTNNAC 8 cut(s) 312, 792, 1476, 2115, 2478, 2534, 2976, 3666
Hpy8I GTNNAC 8 cut(s) 312, 792, 1476, 2115, 2478, 2534, 2976, 3666
Hpy99I CGWCG 4 cut(s) 19, 25, 95, 98
HpyCH4IV ACGT 5 cut(s) 1556, 1986, 2431, 2791, 4071
HpySE526I ACGT 5 cut(s) 1556, 1986, 2431, 2791, 4071
HspAI GCGC 3 cut(s) 3368, 4485, 4513
Kpn2I TCCGGA 3 cut(s) 1941, 2488, 4026
KpnI GGTACC 1 cut(s) 2682
Ksp22I TGATCA 2 cut(s) 3354, 4132
KspI CCGCGG 1 cut(s) 412
MaeI CTAG 7 cut(s) 473, 1062, 1260, 2657, 3249, 3450, 4476
MaeII ACGT 5 cut(s) 1556, 1986, 2431, 2791, 4071
MbiI CCGCTC 1 cut(s) 782
MflI RGATCY 1 cut(s) 1944
MlyI GAGTC 3 cut(s) 1799, 1904, 4024
MmeI TCCRAC 4 cut(s) 1081, 2297, 3271, 4382
Mph1103I ATGCAT 1 cut(s) 3071
MroI TCCGGA 3 cut(s) 1941, 2488, 4026
MroXI GAANNNNTTC 2 cut(s) 2259, 4344
MspCI CTTAAG 1 cut(s) 3208
Mva1269I GAATGC 3 cut(s) 825, 928, 3015
MvaI CCWGG 4 cut(s) 130, 1112, 1949, 2358
MvnI CGCG 1 cut(s) 411
NciI CCSGG 6 cut(s) 36, 479, 749, 1432, 2898, 2933
NdeI CATATG 2 cut(s) 2448, 3403
NmuCI GTSAC 6 cut(s) 259, 1751, 2368, 2792, 2831, 3941
NsiI ATGCAT 1 cut(s) 3071
NspI RCATGY 8 cut(s) 399, 978, 2030, 2330, 3162, 4091, 4115, 4415
OliI CACNNNNGTG 3 cut(s) 1758, 3039, 3948
PaeI GCATGC 2 cut(s) 2330, 4415
PaqCI CACCTGC 2 cut(s) 556, 2740
PasI CCCWGGG 1 cut(s) 129
PceI AGGCCT 1 cut(s) 2386
PciI ACATGT 2 cut(s) 974, 3158
PctI GAATGC 3 cut(s) 825, 928, 3015
PdmI GAANNNNTTC 2 cut(s) 2259, 4344
PflMI CCANNNNNTGG 2 cut(s) 1100, 3290
PfoI TCCNGGA 3 cut(s) 1110, 1947, 2896
PleI GAGTC 3 cut(s) 1798, 1903, 4024
PpsI GAGTC 3 cut(s) 1798, 1903, 4024
Ppu21I YACGTR 1 cut(s) 2792
PscI ACATGT 2 cut(s) 974, 3158
PshAI GACNNNNGTC 4 cut(s) 1545, 2120, 3735, 4205
PsiI TTATAA 1 cut(s) 3549
Psp124BI GAGCTC 3 cut(s) 624, 2808, 2968
Psp6I CCWGG 4 cut(s) 128, 1110, 1947, 2356
PspFI CCCAGC 4 cut(s) 1203, 3393, 3480, 3621
PspGI CCWGG 4 cut(s) 128, 1110, 1947, 2356
PspPI GGNCC 9 cut(s) 33, 113, 1371, 1547, 1666, 1778, 1883, 3737, 3856
PstI CTGCAG 5 cut(s) 680, 2158, 2606, 2864, 4243
PstNI CAGNNNCTG 2 cut(s) 2928, 3225
PsuI RGATCY 1 cut(s) 1944
PvuII CAGCTG 9 cut(s) 532, 1658, 1901, 1963, 2607, 2716, 3222, 3848, 4048
RsaI GTAC 5 cut(s) 73, 665, 2500, 2680, 2849
RsaNI GTAC 5 cut(s) 72, 664, 2499, 2679, 2848
SacI GAGCTC 3 cut(s) 624, 2808, 2968
SacII CCGCGG 1 cut(s) 412
Sau96I GGNCC 9 cut(s) 33, 113, 1371, 1547, 1666, 1778, 1883, 3737, 3856
SbfI CCTGCAGG 2 cut(s) 2158, 4243
SchI GAGTC 3 cut(s) 1799, 1904, 4024
SdaI CCTGCAGG 2 cut(s) 2158, 4243
SfcI CTRYAG 9 cut(s) 676, 1330, 1692, 1797, 2154, 2602, 2860, 3882, 4239
Sfr303I CCGCGG 1 cut(s) 412
SgrBI CCGCGG 1 cut(s) 412
SinI GGWCC 3 cut(s) 113, 1547, 3737
SmlI CTYRAG 9 cut(s) 623, 1024, 1382, 2087, 2807, 2967, 3208, 3572, 3946
SmoI CTYRAG 9 cut(s) 623, 1024, 1382, 2087, 2807, 2967, 3208, 3572, 3946
SphI GCATGC 2 cut(s) 2330, 4415
Sse8387I CCTGCAGG 2 cut(s) 2158, 4243
SseBI AGGCCT 1 cut(s) 2386
SspI AATATT 2 cut(s) 1642, 3832
SspMI CTAG 7 cut(s) 473, 1062, 1260, 2657, 3249, 3450, 4476
SstI GAGCTC 3 cut(s) 624, 2808, 2968
StuI AGGCCT 1 cut(s) 2386
StyI CCWWGG 5 cut(s) 9, 1094, 1344, 3284, 4196
TaiI ACGT 5 cut(s) 1559, 1989, 2434, 2794, 4074
TatI WGTACW 2 cut(s) 663, 2847
TauI GCSGC 5 cut(s) 52, 145, 411, 414, 2598
TseFI GTSAC 6 cut(s) 259, 1751, 2368, 2792, 2831, 3941
Tsp45I GTSAC 6 cut(s) 259, 1751, 2368, 2792, 2831, 3941
TspGWI ACGGA 5 cut(s) 111, 528, 2267, 2850, 4352
Van91I CCANNNNNTGG 2 cut(s) 1100, 3290
Vha464I CTTAAG 1 cut(s) 3208
VneI GTGCAC 2 cut(s) 310, 2476
VpaK11BI GGWCC 3 cut(s) 113, 1547, 3737
XagI CCTNNNNNAGG 1 cut(s) 133
XapI RAATTY 8 cut(s) 81, 1412, 1445, 1994, 2096, 3635, 4079, 4181
XbaI TCTAGA 4 cut(s) 472, 1061, 2656, 3248
XceI RCATGY 8 cut(s) 399, 978, 2030, 2330, 3162, 4091, 4115, 4415
XcmI CCANNNNNNNNNTGG 4 cut(s) 1218, 1331, 3408, 3521
XmiI GTMKAC 3 cut(s) 1475, 2533, 3665
XmnI GAANNNNTTC 2 cut(s) 2259, 4344
XspI CTAG 7 cut(s) 473, 1062, 1260, 2657, 3249, 3450, 4476
Zsp2I ATGCAT 1 cut(s) 3071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.