RLG00000001920

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000280
Physical Location & Seq
Reverse (-)
23027740 .. 23032374
4635 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001920

Sequence Viewer

Length: 4485 bp
ATGAGAATCCAGGCGAGATTGTGGCCCCGAGGCACAAAGCGGCCGAACATATGGGGAATTCAGAACGACGACGGAACAAATGGGGAAGAAGATGGTGGAGGACCGTCTGTGGAGCCACTGCCAAAGCTTGTTCCCTGGGGGAGGAAGCGGCGGAGGCTAGGATGCGAGGGGTTTTGGGCAAATCAAAATGAAGGAAAGGCAAGGGCTGTGAAGGCGAAGGCGAAGAAGAAGAAGAAGCAGCAGCAGCAGCAGCAGAGAAGGGCTCCATTGGAGGTGCAGCAGCAGCAGCAGACAAGGGACGAACAGGTACCACTACCTAGTTACCTGCAGAGAAGGGCTCCCTTGAAGGTACGGACAATCAAGTGCCATCAATGTAAGCAAAATGATATGGGAAGAGCGGTGCGGTGCACAAAGTGTTCGAGGAAGAGGTATTGTATCCCTTGCATAAGGACTTGGTATCCCAACACCTCAGAGGAAGCCATAGCTGAGGCTTGTCCTGCATGCCGCGGCAACTGCAACTGCAATGTCTGCCTTTGTTGGGGTGTGCCTGTCAGGAATCTGAACCCGAAAATCGACAAGGAGACCAAGCTGGAGCACTCTAAGTATTTGGTTCATCAGCTGCTTCCGTATTTGAAGATGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTGGAAGCCAAGAGGCAGGGGTTAGGTGATCTTTCGGAGCTCAAGGTGGACAAGGCTAAAAGCGGTGACCAAGTGCGTATGTACTGCAAGAGCTGCAGAACTTCGATTTTTGATTTTCATAGGACCTGTCCCGGGTGCGAGTATGATCTGTGCCTCGGCTGCTGCCGGGAGATTCGAGATGGCAAGTGGAAGAAGAGAGCTCAAGCGTCCACATTTGAGTGGAAATGGAGAGCAAGTGAAGATGGAAGCATTCTCTGCCCCCCAACACACATGAAAGGTTGTGGTAAGCATAATTTAGAATTGAGATGCATTTTTCCTCAGAATCATGTCATGGAGTTGGTAGAGAAAGCAGAAGAAATAGATGCAAGTTACAATTTTTTGCCTGATTGTAGTGAAACATGTGCATATAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTCAAGTAGTAGCAGCAGCTGTAAGTTAAGGAAAGCAGCTTCTAGAGTTGCTTCTGATACTGACTACTTGTTCTGTCCAAGGGTTGGACAAATTCAAGATAAGGGTTTTGGGCATTTTCAGTGGCATTGGAACAGGGGCGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCTGTCATGTGGCGGGCATTTAGACAGATAGAGAAAGTCAAGAATGATAGTGAATTGACATTCCACGCCATTGATTGTTGGGATTGGTGTTTTATAGAAATCAACATCCTCCATTTCTTTAGTGGATATTCCAACGGACGGTCTCATAAGACAGGATTGCCTCAGAATCTCAAGCTCAATGACTTCCCATCATCTGCTGAATTTTACAAACGTCTTCCCCGGCATTGTAAGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATACTCACCCCAACAAGGGAACTTTGAACCTTGCTACCTACTTGCCACCTACCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACAGGGAGATTCCGTAACCAAGCTTCACTGCAATATGTATGATGCGGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACAACTGAACAACTTGCAACTATAGAGAAGTTGAAGAAAAAGCACAGGGAGCAAAACGAAAGAGAGATATTTGGGGATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCTCAGCAAGAGTCAGCTATAGAGAAGTTGAAGAGAAAGTATAGGGAGCAAAACAAAAGAGAGATATTGGGGAATTGTCACACAAGTGCTGGAAATGGGAGTGGGCCTCAGGAAGAGTCACATGAAGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGATCCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCACGTCAATGAGTTTATGCATGTACATGGTAATCCTTTGCAGCATGTTACTCATGCTATACATGATCAGACTGTCTATCTCACTGTGGAACACAAGAGGAAGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATGCTGTTTTCATTCCTGCTGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTGGACTTTGTTTCCCCTGAGAGTGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACAAACCACAGAGCCCATGAGGACAAACTGGAGGTGAAGAAAATGATTGTGCATGCGGTTTGTGAAGCTATGAAGGAGATATCTGGTGAGAATGTGACAAAGAGGAGATGGTATCCCAACACATCAGAGGAAGCCATTGCTAAGGCTTGTCCTGTATGCCACGGCAACTGCAACTGCACAGCCTGCCTTCGTTTGGATGTGCCTGCCAGGTGTTTGCAGAATCTAGACCCCAAAATCGACAGGGGTACCAAGCTGGAGCACTGTAAGTATTTGGTTCATCAGCTGCTTCCCTATTTGAAGAGGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTCGAGGCTAAGAGGCGGGGATTACGTGATGTTTTGGAGCTCAAGGTGGAGAAGGCTAAGTGCGGTGAAGATGAGCGTATGTACTGCAACAACTGCAGCACTTCGATTTTTGATTTTCATAGGAGTTGTCCCAAGTGTGGGTATGATCTGTGCCTCAGTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAAGAAGAGAGAGGTGGAGGAGGAAGCTCAAGTGTCGTCATTTGAGTGGAGATTTAGTGAAGATGGAAGCATTCTCTGCCCCCCAACACAAAAGAAATGCTTTGGTAAGCATAATTTAGAATTGAGATGCATTTTTTCTCAGAATCATGTCATGGAATTGGTAGAGAAAGCAGGAAAAATAGATGCGAGTTACAATTTCTTACCTGATCGTAGTGAAACATGTGCATACAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTCAAGCAGCAGCAACTGTAAGTTAAGGAAAGCAGCTTCTAGAGTTGCTTCTGATAGTAACTACTTGTTCTGCCCAAGGGTTGGACAAATCCAAGATAAGGATTTTGGGCATTTTCAGTGGCATTGGATGAGGGGCGAGCCGGTGGTCGTTAGCAATGCATTTGAAAGTGGATCAGGTTTAAGCTGGGAACCATCCGTCATGTGGCGGGCACTTAGAAAGACAGTTAATAACTACCATGCTAGTGAATTGACCTTCCACGCCGTTGATTGCTGGGATTGGTGTTTTATAAGAATCAAAATCCGCCATTTCTTTAGTGGATATTCCAAAGGACGGTCTTATAAGACAGGATGGCCTCAGAATCTCAAGGTCAATGACTTCCCATTATCTACTGAGTTTCACAATCGTCTTCCCCGGCATGGTAGGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATAGTCACCCAAACAAGGGAACTCTGAACCTTGCTACCTACTTGCCACCTACATCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTACGGTTTTGCACAGGAGCTTGGACAGGGAGATTCTGTAACCAAGCTTCACTGCAATATGTCTGATGTGGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACAACTGAACAACTTGCAACTATAGAGAAGTTGAAGAAAAAGCACAGGGAGCAAGACAGAAGAGAGATATTTGGGAATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCTCTGCAAGAATTAGCTGAAGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGACTCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTATGCATGTGTTCTGTAATCCTTTGCAGCATGTTACTCATGCTATACATGATCAGACTGTTTATCTCACTGTGGAGCACAAGAGGAGGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATGCTGTTTTTATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTGTCAAAGTTGCACTGGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAGGAGTTTCGCACACTTCCGACAAACCACAGAGCCCATGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTGTGAAGGAGATTTCTGGTGAGAATGTGATGACTGAGGATGCGAAGTCTGAGGGAGGCGCAAGGAAATTAAGGCAAGGTGTAAGGCGCATGTCTTACAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1495

Amino Acids

170.95

Weight (kDa)

8.62

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 119 - 178 5.9e-07 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 676 - 771 6.2e-13 JmjC domain, hydroxylase
JmjC PF02373 1321 - 1417 2.7e-13 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3449, 3501
AarI CACCTGC 2 cut(s) 643, 2680
Acc36I ACCTGC 4 cut(s) 333, 643, 2680, 4184
Acc65I GGTACC 2 cut(s) 307, 2618
AccB1I GGYRCC 4 cut(s) 307, 2021, 2618, 3959
AccB7I CCANNNNNTGG 2 cut(s) 1199, 3242
AccBSI CCGCTC 1 cut(s) 398
AccI GTMKAC 1 cut(s) 1574
AccII CGCG 1 cut(s) 507
AccIII TCCGGA 2 cut(s) 2040, 3978
AclWI GGATC 4 cut(s) 1297, 2038, 2051, 3340
AcoI YGGCCR 1 cut(s) 41
AcsI RAATTY 8 cut(s) 57, 1206, 1511, 1544, 2195, 3587, 4031, 4133
AcuI CTGAAG 3 cut(s) 1779, 3822, 3960
AdeI CACNNNGTG 1 cut(s) 414
AfaI GTAC 6 cut(s) 309, 351, 752, 2106, 2620, 2789
AflIII ACRYGT 2 cut(s) 1067, 3113
AjiI CACGTC 2 cut(s) 2086, 4024
AjnI CCWGG 4 cut(s) 9, 134, 2046, 2579
AleI CACNNNNGTG 3 cut(s) 1857, 1962, 3900
Alw21I GWGCWC 7 cut(s) 410, 597, 711, 871, 2634, 2748, 4113
Alw26I GTCTC 2 cut(s) 575, 1458
Alw44I GTGCAC 1 cut(s) 406
AlwI GGATC 4 cut(s) 1297, 2038, 2051, 3340
AlwNI CAGNNNCTG 3 cut(s) 1134, 2868, 3177
Ama87I CYCGRG 2 cut(s) 27, 800
Aor13HI TCCGGA 2 cut(s) 2040, 3978
AoxI GGCC 8 cut(s) 23, 41, 1764, 1877, 1982, 3512, 3807, 3920
ApaLI GTGCAC 1 cut(s) 406
ApoI RAATTY 8 cut(s) 57, 1206, 1511, 1544, 2195, 3587, 4031, 4133
Asp700I GAANNNNTTC 1 cut(s) 2358
Asp718I GGTACC 2 cut(s) 307, 2618
AspLEI GCGC 2 cut(s) 4439, 4467
AsuC2I CCSGG 6 cut(s) 801, 802, 836, 1531, 2873, 3574
AvaI CYCGRG 2 cut(s) 27, 800
AvaII GGWCC 4 cut(s) 101, 792, 1646, 3689
AxyI CCTNAGG 1 cut(s) 1986
BaeGI GKGCMC 2 cut(s) 410, 3373
BaeI ACNNNNGTAYC 2 cut(s) 2602, 2635
BamHI GGATCC 1 cut(s) 2043
BanI GGYRCC 4 cut(s) 307, 2021, 2618, 3959
BanII GRGCYC 7 cut(s) 265, 340, 711, 871, 2389, 2748, 4327
BarI GAAGNNNNNNTAC 4 cut(s) 1695, 1727, 3738, 3770
BbsI GAAGAC 2 cut(s) 1517, 3560
Bbv12I GWGCWC 7 cut(s) 410, 597, 711, 871, 2634, 2748, 4113
BbvCI CCTCAGC 2 cut(s) 486, 1881
BceAI ACGGC 6 cut(s) 1094, 2194, 2551, 3140, 3407, 4132
BciT130I CCWGG 4 cut(s) 11, 136, 2048, 2581
BciVI GTATCC 3 cut(s) 446, 468, 2496
BclI TGATCA 2 cut(s) 2146, 4084
BcnI CCSGG 6 cut(s) 801, 802, 836, 1531, 2873, 3574
BcoDI GTCTC 2 cut(s) 575, 1458
BfaI CTAG 6 cut(s) 158, 318, 1158, 2597, 3201, 3402
BfmI CTRYAG 7 cut(s) 326, 763, 1791, 1896, 2800, 3834, 4191
BfuAI ACCTGC 4 cut(s) 333, 643, 2680, 4184
BfuI GTATCC 3 cut(s) 446, 468, 2496
Bme18I GGWCC 4 cut(s) 101, 792, 1646, 3689
BmeT110I CYCGRG 2 cut(s) 27, 800
BmgBI CACGTC 2 cut(s) 2086, 4024
BoxI GACNNNNGTC 4 cut(s) 1644, 2219, 3687, 4157
BpiI GAAGAC 2 cut(s) 1517, 3560
BplI GAGNNNNNCTC 6 cut(s) 247, 279, 322, 354, 1969, 2001
BpmI CTGGAG 6 cut(s) 611, 2084, 2423, 2648, 4022, 4361
Bpu10I CCTNAGC 3 cut(s) 486, 1881, 2514
BpuMI CCSGG 6 cut(s) 801, 802, 836, 1531, 2873, 3574
BsaAI YACGTR 1 cut(s) 2732
BsaI GGTCTC 2 cut(s) 575, 1458
BsaWI WCCGGW 2 cut(s) 2040, 3978
Bse118I RCCGGY 1 cut(s) 3301
Bse1I ACTGG 3 cut(s) 2310, 2406, 4248
Bse21I CCTNAGG 1 cut(s) 1986
Bse3DI GCAATG 4 cut(s) 529, 1279, 2508, 3322
BseAI TCCGGA 2 cut(s) 2040, 3978
BseBI CCWGG 4 cut(s) 11, 136, 2048, 2581
BseMI GCAATG 4 cut(s) 529, 1279, 2508, 3322
BseNI ACTGG 3 cut(s) 2310, 2406, 4248
BseRI GAGGAG 4 cut(s) 2491, 2927, 4132, 4307
BseSI GKGCMC 2 cut(s) 410, 3373
BseX3I CGGCCG 1 cut(s) 41
BseYI CCCAGC 3 cut(s) 1302, 3345, 3432
BsgI GTGCAG 2 cut(s) 296, 2533
Bsh1236I CGCG 1 cut(s) 507
Bsh1285I CGRYCG 1 cut(s) 44
BshFI GGCC 8 cut(s) 25, 43, 1766, 1879, 1984, 3514, 3809, 3922
BshNI GGYRCC 4 cut(s) 307, 2021, 2618, 3959
BsiEI CGRYCG 1 cut(s) 44
BsiHKAI GWGCWC 7 cut(s) 410, 597, 711, 871, 2634, 2748, 4113
BsiHKCI CYCGRG 2 cut(s) 27, 800
BslFI GGGAC 5 cut(s) 311, 783, 2045, 2820, 3983
BsmAI GTCTC 2 cut(s) 575, 1458
BsmFI GGGAC 5 cut(s) 311, 783, 2045, 2820, 3983
BsmI GAATGC 2 cut(s) 918, 2964
BsnI GGCC 8 cut(s) 25, 43, 1766, 1879, 1984, 3514, 3809, 3922
Bso31I GGTCTC 2 cut(s) 575, 1458
BsoBI CYCGRG 2 cut(s) 27, 800
Bsp13I TCCGGA 2 cut(s) 2040, 3978
Bsp1407I TGTACA 1 cut(s) 2104
BspANI GGCC 8 cut(s) 25, 43, 1766, 1879, 1984, 3514, 3809, 3922
BspEI TCCGGA 2 cut(s) 2040, 3978
BspFNI CGCG 1 cut(s) 507
BspMAI CTGCAG 4 cut(s) 330, 767, 2804, 4195
BspMI ACCTGC 4 cut(s) 333, 643, 2680, 4184
BspPI GGATC 4 cut(s) 1297, 2038, 2051, 3340
BspQI GCTCTTC 1 cut(s) 388
BspT107I GGYRCC 4 cut(s) 307, 2021, 2618, 3959
BspTNI GGTCTC 2 cut(s) 575, 1458
BsrBI CCGCTC 1 cut(s) 398
BsrDI GCAATG 4 cut(s) 529, 1279, 2508, 3322
BsrFI RCCGGY 1 cut(s) 3301
BsrGI TGTACA 1 cut(s) 2104
BsrI ACTGG 3 cut(s) 2310, 2406, 4248
BssAI RCCGGY 1 cut(s) 3301
BssNAI GTATAC 1 cut(s) 1575
BssT1I CCWWGG 2 cut(s) 1193, 3236
Bst1107I GTATAC 1 cut(s) 1575
Bst2UI CCWGG 4 cut(s) 11, 136, 2048, 2581
Bst6I CTCTTC 9 cut(s) 388, 419, 857, 1904, 1986, 2349, 2666, 2894, 3868
BstAPI GCANNNNNTGC 6 cut(s) 528, 762, 924, 2556, 2799, 2970
BstAUI TGTACA 1 cut(s) 2104
BstBAI YACGTR 1 cut(s) 2732
BstC8I GCNNGC 9 cut(s) 502, 1256, 1326, 2427, 2557, 2577, 3299, 3369, 4365
BstDSI CCRYGG 4 cut(s) 505, 2210, 2533, 4148
BstEII GGTNACC 1 cut(s) 734
BstENI CCTNNNNNAGG 1 cut(s) 139
BstFNI CGCG 1 cut(s) 507
BstHHI GCGC 2 cut(s) 4439, 4467
BstMAI GTCTC 2 cut(s) 575, 1458
BstMCI CGRYCG 1 cut(s) 44
BstNI CCWGG 4 cut(s) 11, 136, 2048, 2581
BstPAI GACNNNNGTC 4 cut(s) 1644, 2219, 3687, 4157
BstPI GGTNACC 1 cut(s) 734
BstSFI CTRYAG 7 cut(s) 326, 763, 1791, 1896, 2800, 3834, 4191
BstSLI GKGCMC 2 cut(s) 410, 3373
BstUI CGCG 1 cut(s) 507
BstV2I GAAGAC 2 cut(s) 1517, 3560
BstX2I RGATCY 1 cut(s) 2043
BstXI CCANNNNNNTGG 2 cut(s) 2231, 4169
BstYI RGATCY 1 cut(s) 2043
BstZ17I GTATAC 1 cut(s) 1575
BstZI CGGCCG 1 cut(s) 41
Bsu36I CCTNAGG 1 cut(s) 1986
BsuI GTATCC 3 cut(s) 446, 468, 2496
BsuRI GGCC 8 cut(s) 25, 43, 1766, 1879, 1984, 3514, 3809, 3922
BtgI CCRYGG 4 cut(s) 505, 2210, 2533, 4148
BtrI CACGTC 2 cut(s) 2086, 4024
BtsI GCAGTG 3 cut(s) 116, 1714, 3757
BveI ACCTGC 4 cut(s) 333, 643, 2680, 4184
Cac8I GCNNGC 9 cut(s) 502, 1256, 1326, 2427, 2557, 2577, 3299, 3369, 4365
CaiI CAGNNNCTG 3 cut(s) 1134, 2868, 3177
CfoI GCGC 2 cut(s) 4439, 4467
Cfr10I RCCGGY 1 cut(s) 3301
Cfr42I CCGCGG 1 cut(s) 508
Cfr9I CCCGGG 1 cut(s) 800
CseI GACGC 1 cut(s) 864
Csp6I GTAC 6 cut(s) 308, 350, 751, 2105, 2619, 2788
CviQI GTAC 6 cut(s) 308, 350, 751, 2105, 2619, 2788
DraIII CACNNNGTG 1 cut(s) 414
EaeI YGGCCR 1 cut(s) 41
EagI CGGCCG 1 cut(s) 41
Eam1104I CTCTTC 9 cut(s) 388, 419, 857, 1904, 1986, 2349, 2666, 2894, 3868
EarI CTCTTC 9 cut(s) 388, 419, 857, 1904, 1986, 2349, 2666, 2894, 3868
EciI GGCGGA 2 cut(s) 166, 3452
Ecl136II GAGCTC 3 cut(s) 709, 869, 2746
EclXI CGGCCG 1 cut(s) 41
Eco130I CCWWGG 2 cut(s) 1193, 3236
Eco24I GRGCYC 7 cut(s) 265, 340, 711, 871, 2389, 2748, 4327
Eco31I GGTCTC 2 cut(s) 575, 1458
Eco32I GATATC 1 cut(s) 2454
Eco47I GGWCC 4 cut(s) 101, 792, 1646, 3689
Eco52I CGGCCG 1 cut(s) 41
Eco53kI GAGCTC 3 cut(s) 709, 869, 2746
Eco57I CTGAAG 3 cut(s) 1779, 3822, 3960
Eco81I CCTNAGG 1 cut(s) 1986
Eco88I CYCGRG 2 cut(s) 27, 800
Eco91I GGTNACC 1 cut(s) 734
EcoICRI GAGCTC 3 cut(s) 709, 869, 2746
EcoNI CCTNNNNNAGG 1 cut(s) 139
EcoO109I RGGNCCY 1 cut(s) 792
EcoO65I GGTNACC 1 cut(s) 734
EcoRI GAATTC 3 cut(s) 57, 2195, 4133
EcoRII CCWGG 4 cut(s) 9, 134, 2046, 2579
EcoRV GATATC 1 cut(s) 2454
EcoT14I CCWWGG 2 cut(s) 1193, 3236
EcoT22I ATGCAT 5 cut(s) 980, 2103, 3026, 3322, 4041
EcoT38I GRGCYC 7 cut(s) 265, 340, 711, 871, 2389, 2748, 4327
ErhI CCWWGG 2 cut(s) 1193, 3236
FalI AAGNNNNNCTT 6 cut(s) 1752, 1784, 2978, 3010, 3795, 3827
FaqI GGGAC 5 cut(s) 311, 783, 2045, 2820, 3983
FauI CCCGC 3 cut(s) 1317, 2715, 3360
FauNDI CATATG 1 cut(s) 50
FbaI TGATCA 2 cut(s) 2146, 4084
FblI GTMKAC 1 cut(s) 1574
FriOI GRGCYC 7 cut(s) 265, 340, 711, 871, 2389, 2748, 4327
FspBI CTAG 6 cut(s) 158, 318, 1158, 2597, 3201, 3402
GlaI GCGC 2 cut(s) 4438, 4466
GsaI CCCAGC 3 cut(s) 1306, 3349, 3436
GsuI CTGGAG 6 cut(s) 611, 2084, 2423, 2648, 4022, 4361
HaeIII GGCC 8 cut(s) 25, 43, 1766, 1879, 1984, 3514, 3809, 3922
HgaI GACGC 1 cut(s) 864
HhaI GCGC 2 cut(s) 4439, 4467
Hin6I GCGC 2 cut(s) 4437, 4465
HinP1I GCGC 2 cut(s) 4437, 4465
HindIII AAGCTT 3 cut(s) 125, 1709, 3752
Hpy166II GTNNAC 6 cut(s) 408, 718, 879, 1575, 2214, 4152
Hpy8I GTNNAC 6 cut(s) 408, 718, 879, 1575, 2214, 4152
Hpy99I CGWCG 2 cut(s) 71, 74
HpyCH4IV ACGT 4 cut(s) 1522, 2085, 2731, 4023
HpySE526I ACGT 4 cut(s) 1522, 2085, 2731, 4023
HspAI GCGC 2 cut(s) 4437, 4465
Kpn2I TCCGGA 2 cut(s) 2040, 3978
KpnI GGTACC 2 cut(s) 311, 2622
Ksp22I TGATCA 2 cut(s) 2146, 4084
KspI CCGCGG 1 cut(s) 508
LguI GCTCTTC 1 cut(s) 388
MaeI CTAG 6 cut(s) 158, 318, 1158, 2597, 3201, 3402
MaeII ACGT 4 cut(s) 1522, 2085, 2731, 4023
MbiI CCGCTC 1 cut(s) 398
MflI RGATCY 1 cut(s) 2043
MlyI GAGTC 3 cut(s) 1898, 2003, 3976
MmeI TCCRAC 5 cut(s) 1180, 1467, 2396, 3223, 4334
Mph1103I ATGCAT 5 cut(s) 980, 2103, 3026, 3322, 4041
MroI TCCGGA 2 cut(s) 2040, 3978
MroXI GAANNNNTTC 1 cut(s) 2358
MspA1I CMGCKG 8 cut(s) 507, 619, 1134, 1757, 2062, 2656, 3800, 4000
Mva1269I GAATGC 2 cut(s) 918, 2964
MvaI CCWGG 4 cut(s) 11, 136, 2048, 2581
MvnI CGCG 1 cut(s) 507
NciI CCSGG 6 cut(s) 801, 802, 836, 1531, 2873, 3574
NdeI CATATG 1 cut(s) 50
NmeAIII GCCGAG 1 cut(s) 804
NmuCI GTSAC 7 cut(s) 734, 1850, 1955, 1995, 2467, 3620, 3893
NsiI ATGCAT 5 cut(s) 980, 2103, 3026, 3322, 4041
OliI CACNNNNGTG 3 cut(s) 1857, 1962, 3900
PaeI GCATGC 3 cut(s) 504, 2429, 4367
PaqCI CACCTGC 2 cut(s) 643, 2680
PasI CCCWGGG 1 cut(s) 135
PciI ACATGT 2 cut(s) 1067, 3113
PciSI GCTCTTC 1 cut(s) 388
PctI GAATGC 2 cut(s) 918, 2964
PdmI GAANNNNTTC 1 cut(s) 2358
PflMI CCANNNNNTGG 2 cut(s) 1199, 3242
PfoI TCCNGGA 1 cut(s) 2046
PleI GAGTC 3 cut(s) 1897, 2002, 3976
PpsI GAGTC 3 cut(s) 1897, 2002, 3976
Ppu21I YACGTR 1 cut(s) 2732
PpuMI RGGWCCY 1 cut(s) 792
PscI ACATGT 2 cut(s) 1067, 3113
PshAI GACNNNNGTC 4 cut(s) 1644, 2219, 3687, 4157
PsiI TTATAA 2 cut(s) 3449, 3501
Psp124BI GAGCTC 3 cut(s) 711, 871, 2748
Psp5II RGGWCCY 1 cut(s) 792
Psp6I CCWGG 4 cut(s) 9, 134, 2046, 2579
PspEI GGTNACC 1 cut(s) 734
PspFI CCCAGC 3 cut(s) 1302, 3345, 3432
PspGI CCWGG 4 cut(s) 9, 134, 2046, 2579
PspPPI RGGWCCY 1 cut(s) 792
PstI CTGCAG 4 cut(s) 330, 767, 2804, 4195
PstNI CAGNNNCTG 3 cut(s) 1134, 2868, 3177
PsuI RGATCY 1 cut(s) 2043
PvuII CAGCTG 7 cut(s) 619, 1134, 1757, 2062, 2656, 3800, 4000
RsaI GTAC 6 cut(s) 309, 351, 752, 2106, 2620, 2789
RsaNI GTAC 6 cut(s) 308, 350, 751, 2105, 2619, 2788
SacI GAGCTC 3 cut(s) 711, 871, 2748
SacII CCGCGG 1 cut(s) 508
SapI GCTCTTC 1 cut(s) 388
SbfI CCTGCAGG 1 cut(s) 4195
SchI GAGTC 3 cut(s) 1898, 2003, 3976
SdaI CCTGCAGG 1 cut(s) 4195
SfcI CTRYAG 7 cut(s) 326, 763, 1791, 1896, 2800, 3834, 4191
Sfr303I CCGCGG 1 cut(s) 508
SgrBI CCGCGG 1 cut(s) 508
SinI GGWCC 4 cut(s) 101, 792, 1646, 3689
SmaI CCCGGG 1 cut(s) 802
SphI GCATGC 3 cut(s) 504, 2429, 4367
Sse8387I CCTGCAGG 1 cut(s) 4195
SspI AATATT 2 cut(s) 1741, 3784
SspMI CTAG 6 cut(s) 158, 318, 1158, 2597, 3201, 3402
SstI GAGCTC 3 cut(s) 711, 871, 2748
StyI CCWWGG 2 cut(s) 1193, 3236
TaiI ACGT 4 cut(s) 1525, 2088, 2734, 4026
TatI WGTACW 3 cut(s) 750, 2104, 2787
TauI GCSGC 4 cut(s) 43, 151, 507, 510
TseFI GTSAC 7 cut(s) 734, 1850, 1955, 1995, 2467, 3620, 3893
Tsp45I GTSAC 7 cut(s) 734, 1850, 1955, 1995, 2467, 3620, 3893
TspGWI ACGGA 8 cut(s) 87, 367, 615, 1461, 1690, 2366, 3346, 4304
TspMI CCCGGG 1 cut(s) 800
Van91I CCANNNNNTGG 2 cut(s) 1199, 3242
VneI GTGCAC 1 cut(s) 406
VpaK11BI GGWCC 4 cut(s) 101, 792, 1646, 3689
XagI CCTNNNNNAGG 1 cut(s) 139
XapI RAATTY 8 cut(s) 57, 1206, 1511, 1544, 2195, 3587, 4031, 4133
XbaI TCTAGA 3 cut(s) 1157, 2596, 3200
XcmI CCANNNNNNNNNTGG 4 cut(s) 1317, 1430, 3360, 3473
XmaI CCCGGG 1 cut(s) 800
XmiI GTMKAC 1 cut(s) 1574
XmnI GAANNNNTTC 1 cut(s) 2358
XspI CTAG 6 cut(s) 158, 318, 1158, 2597, 3201, 3402
Zsp2I ATGCAT 5 cut(s) 980, 2103, 3026, 3322, 4041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.