Rh7CG382800

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000280
Physical Location & Seq
Forward (+)
48230019 .. 48234497
4479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG382800.1

Sequence Viewer

Length: 4479 bp
ATGGAATTCCAGGCCACGGCGGCGAGATTGAGGCCCCGAGGCACAAAGCGGCCGAACGGCGGTGGTGTGGTGGAGAAGCAGGCCGAGGGTATTTTGGGAATTCAGAACGACGACGGAACAATTGGGGAAGACGTGGAGCCAGCAGCGCCGAAGCTCGTTCACTGGGGGAGGAAGCGGCGGAGGACAGGATGCGAGGGTATTTTGGGAAATCAAAATGAAGGAGAGGCAAGGGCGGCAAATTGGTGCACAAGGGCTCCCTTGGACGTGGGGAAATGTTGCCATCAATGTAAGCGAAATTGTAAGCGAAGAGGGGTGCAGTGCACAAGGTGTCCCGAAAGAGGTAAGCGAAATAGGTATTGTCTCCCTTGCATACGGAGATGGTATCCCAACACCTCACAGGAAGCCATAGCTGAGGCTTGTCCTGTGTGCCGCGGCAACTGCAACTGCACAGCCTGCCTTCGGTTGGGTGCGCGTTTCAGGCGTTTCGAGAATCGAGACCCGAAAATCGATGAGGAGACCAAGCTGGAGCACTCTAAGTATTTGGTTCATCAGCTGCTCCCGTATTTGAAGATGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTGGAAGCCAAGAGGCAGGGGTTACGTGACCTTTTCGAGCTCCAGGTGGACAAGGCTAATTGCGGTGACGGAGTGCGTATGTACTGCAACAGCTGCAGCACTTCGATTTTTGATTTTCATAGGACTTGTCCCGGATGTGAGTATGATCTGTGCCTCGGTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAACAAGAGAGCTCAAGAGTCCACATTTGAGTGGAAATGGAGAGCAAGTGAAGATGGAAGCATTCTCTGCCCCCCAACACACATGAAAGGTTGTGGTAAGCATAATTTAGAATTGAGATGCCTTTTTTCTCAGAATCATGTTATGGAATTGGTAGAGAAAGCAGGAGAAATAGATGCAAGTTACAATTTTTTGCCTGATCGTAGTGAAACATGTGCATATAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTCAAGTAGTGGCAGCAGCAGCTGTAAGTTAAGGAAAGCAGCTTCTAGAGTTGCTTCTGATAGTGACTACTTGTTCTGTCCTAGAGTTGGACAAATCCAAGATAAGGATTTTGGGCATTTTCAGTGGCATTGGAAGAGGGGGGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCTGTCATGTGGCGGGCATTTAGACAGATAAAGAAAATCAAGCATGATAGTGATTTGACAGTCCCCGCCATTGATTGCTGGAATTGGTGTTTTATAGAAATCAACCTCCTCCATTTCTTTAATGGCTATTCCAACGGACGGTCTTATGAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATCATCTACCGAGTTTCACAATCGTCTTCCCCGGCATGGTGAGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATACTCACCCCAACAAGGGAACTCTGAACCTTGCTATCTACTTGCCACCTACCTCAGTAAAGCCAGACATGGGTCCAAAAACATATATTGCCTATGGTTTTACACAGGAGCTTGAACGGGGAGATTCTGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTCAATATTCTGACACATTCAGCTGAAGTGGCCCTTACAACTAAACAACTGGCAACTATAGAGAAGTTGAAGAAAAAGCACAGGGAGCAAGACGAAAGAGAGATATTTGGGAATTGTCACACAATTGCTGGAAATGGAAGTGGGCCTCCGCAAGAATCAGCTGAAGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGATTCAGGATGTTCCGCTGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTATGCATGTGCTCTGTAATCCTTTGCAGCATGTTACTCATGCTATACATGATCAGACTGTTTATCTCACTGTAGAGCACAAGAGGCAGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTTCAAAGACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTTTCAAAGTTGCACTGGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACAAACCACAGAGCCAGTGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTATTAAGGAGATTTCTGGTGAGAATGTGATGGTAAAGAGGGCAAGGGATGTGAAGGCAAAGAGGGCAAGGGCTCTGAAGGCAAACAGGGCAAGGGCTCCCTTGGACGTGTCGACAATTTGCCATCAGTGTCAGAGAAATGTTAAGGGAAGAGTGGTGTGGTGCACAAGGTGTGCAAGGAAGAGGTATTGTATTCCTTGCATACAGAGATGGTATCCCAACACATCAGAGGAAGCCATTGCTAAGGCTTGTCCTGTGTGCCGTGGCAACTGCAACTGCACAGCCTGCCTTCGATTGGATGTGCCTGTCAGGCGTTTGCAGAATCTAGACCCGAAAATCGACAAGGGTACCAAGCTGGAGCACTGTAAGTACTTGGTTCATCAGCTGCTTCCCTATTTGAAGAGGATTAGGGATGAGCAGGTGATTGAGATGCTGATCGAGGCTAAGAGGCAGGGATTACGTGATGTTTCGGAGCTCAAGGTGGAGAAGGCTAATTGCGGTGAAGATGAGCGTATGTACTGCAACAACTGCAGCACTTCGATTTTTGATTTTCATAGGAGTTGTCCTGAGTGTGAGTATGATCTGTGCCTCAGTTGCTGCCGGGAGATTCGAGATGGCAGGTGGAAGAAGAGAGGGATGGAGGAGAAAGCTCAAGTGTCCACATTTGAGTGGAGAGCAAGTGAAGATGGAAGCATTCTCTGGACACATATGAAATGCTGTGGTAAGCATAAATTAGAATTGAAATGCAAATTTTCTCAGAATCATGTCATAGAATTGGTAGAGAAAGCAGGAAAAATAGATGAAAGTTACAGTCATTTGCGTGCTAGTAGTGAAACACCTGCACACAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTCAAGTAGCAGCTGCTCTATGTTAAGGAAAGCAGCTTCTAGAGATGGTTCTGATGATAACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAAGATAAGGATTTTGGGCATTTTCAGTGGCATTGGATGAGGGGTGAGCCTGTGATCGTTAGCAATGCACTTGACAATGGATCAGGTTTAAGCTGGGAACCATCTGTCATGTGGCGGGCATTTAGAAAGATAAAGAAGATCAAGCATGATAGTGAATTGACAGTCCACGCCATTGATTGCTGGGATTGGTGTTTTATAGAAATCAACATCCTCCATTTCTTTAGTGGATATTCCAACGGACGGTGTTATAAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATCATCTACTGAGTTTCACAATCGGCTTCCCCGGCATGTTAAGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATACTCACCCCAACAAGGGAACTCTGAACCTTGCTATCTACTTGCCACCTACCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACGGGGAGATTCTGTAACTAAGCTTCACTGCAATATGTCTGATGCGATTAATATTCTGACGCATACAGCTGAAGTGCCCCTTACAACTGAACAACTGGCAACTATAGAGAAGTTGAAGAAAAAGCACAGGGAGCAAGACGAAAGAGAGATATTTGGGAATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCGCAGCAAGAATCCGCTGAAGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGATTCAGGATGTTCCGCTGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTATGCATGTGCTCTGTAATCCTTTGCAGCGTGTTACTCATGCTATACATGATCAGACTGTTTATCTCACTGTGGAGCACAAGAGGAAGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTGGAGTTTGTTTCCCCTGAGAATGTGGGTGCGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACAAACCACAGAGCTAGTGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTGTGAAGGAGATTTCTGCTGAGAATGTGATGACTGAGGTTGTGAAGCCTAGGGGAGGCGCAAGGGAATTAAGGCAAGGTGTAAGGTCCAAGTCTTACAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1492

Amino Acids

170.4

Weight (kDa)

8.5

Isoelectric Point (pI)

49.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 89 - 154 7.7e-06 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 617 - 715 2.6e-13 JmjC domain, hydroxylase
zf-4CXXC_R1 PF10497 795 - 855 6e-07 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 1316 - 1415 5.1e-13 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3495
AarI CACCTGC 4 cut(s) 577, 2680, 2880, 3118
Acc36I ACCTGC 6 cut(s) 577, 2078, 2680, 2880, 3118, 4178
Acc65I GGTACC 1 cut(s) 2618
AccB1I GGYRCC 3 cut(s) 1853, 2618, 3953
AccB7I CCANNNNNTGG 1 cut(s) 3236
AccI GTMKAC 3 cut(s) 1511, 2384, 3611
AccII CGCG 2 cut(s) 432, 472
AccIII TCCGGA 2 cut(s) 1872, 3972
AclWI GGATC 2 cut(s) 1234, 3334
AcoI YGGCCR 1 cut(s) 50
AcsI RAATTY 9 cut(s) 5, 99, 1481, 1925, 2027, 3020, 3581, 4025, 4127
AcuI CTGAAG 5 cut(s) 1716, 1854, 2369, 3816, 3954
AdeI CACNNNGTG 2 cut(s) 327, 2442
AfaI GTAC 4 cut(s) 686, 2620, 2642, 2789
AflIII ACRYGT 2 cut(s) 1001, 2379
AjiI CACGTC 5 cut(s) 133, 265, 1918, 2380, 4018
AjnI CCWGG 2 cut(s) 9, 645
AleI CACNNNNGTG 2 cut(s) 3118, 3894
Alw26I GTCTC 4 cut(s) 365, 489, 509, 1391
Alw44I GTGCAC 3 cut(s) 244, 319, 2434
AlwI GGATC 2 cut(s) 1234, 3334
AlwNI CAGNNNCTG 2 cut(s) 1071, 2868
Ama87I CYCGRG 1 cut(s) 36
Aor13HI TCCGGA 2 cut(s) 1872, 3972
AoxI GGCC 9 cut(s) 12, 32, 50, 81, 1406, 1701, 1814, 3506, 3914
ApaLI GTGCAC 3 cut(s) 244, 319, 2434
ApoI RAATTY 9 cut(s) 5, 99, 1481, 1925, 2027, 3020, 3581, 4025, 4127
AseI ATTAAT 1 cut(s) 3774
Asp700I GAANNNNTTC 2 cut(s) 2190, 4290
Asp718I GGTACC 1 cut(s) 2618
AspA2I CCTAGG 1 cut(s) 4421
AspLEI GCGC 3 cut(s) 148, 472, 4433
AspS9I GGNCC 7 cut(s) 33, 1583, 1702, 1814, 3683, 3914, 4458
AsuC2I CCSGG 5 cut(s) 735, 770, 1468, 2873, 3568
AvaI CYCGRG 1 cut(s) 36
AvaII GGWCC 3 cut(s) 1583, 3683, 4458
AvrII CCTAGG 1 cut(s) 4421
BaeGI GKGCMC 4 cut(s) 248, 323, 2438, 3804
BaeI ACNNNNGTAYC 2 cut(s) 2602, 2635
BanI GGYRCC 3 cut(s) 1853, 2618, 3953
BanII GRGCYC 6 cut(s) 256, 645, 805, 2347, 2371, 2748
BarI GAAGNNNNNNTAC 4 cut(s) 1632, 1664, 3732, 3764
BbsI GAAGAC 2 cut(s) 135, 1454
BbvCI CCTCAGC 1 cut(s) 411
BceAI ACGGC 7 cut(s) 33, 73, 1028, 2026, 2517, 3134, 4126
BciT130I CCWGG 2 cut(s) 11, 647
BciVI GTATCC 2 cut(s) 393, 2496
BclI TGATCA 2 cut(s) 1978, 4078
BcnI CCSGG 5 cut(s) 735, 770, 1468, 2873, 3568
BcoDI GTCTC 4 cut(s) 365, 489, 509, 1391
BfaI CTAG 7 cut(s) 1095, 1131, 2597, 3096, 3195, 4320, 4422
BfmI CTRYAG 7 cut(s) 697, 1728, 1998, 2085, 2800, 3828, 4185
BfoI RGCGCY 1 cut(s) 149
BfuAI ACCTGC 6 cut(s) 577, 2078, 2680, 2880, 3118, 4178
BfuI GTATCC 2 cut(s) 393, 2496
BglI GCCNNNNNGGC 2 cut(s) 20, 2581
BlnI CCTAGG 1 cut(s) 4421
BmcAI AGTACT 1 cut(s) 2642
Bme1390I CCNGG 7 cut(s) 11, 647, 735, 770, 1468, 2873, 3568
Bme18I GGWCC 3 cut(s) 1583, 3683, 4458
BmeT110I CYCGRG 1 cut(s) 36
BmgBI CACGTC 5 cut(s) 133, 265, 1918, 2380, 4018
BmgT120I GGNCC 7 cut(s) 33, 1583, 1702, 1814, 3683, 3914, 4458
BmrFI CCNGG 7 cut(s) 11, 647, 735, 770, 1468, 2873, 3568
BmrI ACTGGG 1 cut(s) 172
BmuI ACTGGG 1 cut(s) 172
BoxI GACNNNNGTC 3 cut(s) 1581, 3681, 4151
BpiI GAAGAC 2 cut(s) 135, 1454
BpmI CTGGAG 8 cut(s) 545, 629, 1916, 2255, 2648, 4016, 4259, 4355
Bpu10I CCTNAGC 2 cut(s) 411, 2514
BpuEI CTTGAG 9 cut(s) 789, 1036, 1403, 2003, 2732, 2907, 3142, 3503, 4103
BpuMI CCSGG 5 cut(s) 735, 770, 1468, 2873, 3568
Bsa29I ATCGAT 1 cut(s) 507
BsaAI YACGTR 2 cut(s) 629, 2732
BsaBI GATNNNNATC 1 cut(s) 2705
BsaI GGTCTC 2 cut(s) 489, 509
BsaWI WCCGGW 2 cut(s) 1872, 3972
Bse1I ACTGG 6 cut(s) 167, 1725, 2142, 2220, 3825, 4242
Bse3DI GCAATG 3 cut(s) 1216, 2508, 3316
Bse8I GATNNNNATC 1 cut(s) 2705
BseAI TCCGGA 2 cut(s) 1872, 3972
BseBI CCWGG 2 cut(s) 11, 647
BseCI ATCGAT 1 cut(s) 507
BseJI GATNNNNATC 1 cut(s) 2705
BseMI GCAATG 3 cut(s) 1216, 2508, 3316
BseNI ACTGG 6 cut(s) 167, 1725, 2142, 2220, 3825, 4242
BseRI GAGGAG 3 cut(s) 527, 1346, 2927
BseSI GKGCMC 4 cut(s) 248, 323, 2438, 3804
BseX3I CGGCCG 1 cut(s) 50
BseYI CCCAGC 3 cut(s) 1239, 3339, 3426
BsgI GTGCAG 4 cut(s) 335, 430, 2533, 3096
Bsh1236I CGCG 2 cut(s) 432, 472
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 9 cut(s) 14, 34, 52, 83, 1408, 1703, 1816, 3508, 3916
BshNI GGYRCC 3 cut(s) 1853, 2618, 3953
BshVI ATCGAT 1 cut(s) 507
BsiEI CGRYCG 1 cut(s) 53
BsiHKCI CYCGRG 1 cut(s) 36
BsiSI CCGG 8 cut(s) 735, 769, 1468, 1873, 2872, 3568, 3674, 3973
BslFI GGGAC 5 cut(s) 315, 717, 1295, 1877, 3977
BsmAI GTCTC 4 cut(s) 365, 489, 509, 1391
BsmFI GGGAC 5 cut(s) 315, 717, 1295, 1877, 3977
BsmI GAATGC 2 cut(s) 852, 2964
BsnI GGCC 9 cut(s) 14, 34, 52, 83, 1408, 1703, 1816, 3508, 3916
Bso31I GGTCTC 2 cut(s) 489, 509
BsoBI CYCGRG 1 cut(s) 36
Bsp13I TCCGGA 2 cut(s) 1872, 3972
BspANI GGCC 9 cut(s) 14, 34, 52, 83, 1408, 1703, 1816, 3508, 3916
BspDI ATCGAT 1 cut(s) 507
BspEI TCCGGA 2 cut(s) 1872, 3972
BspFNI CGCG 2 cut(s) 432, 472
BspMAI CTGCAG 4 cut(s) 701, 2089, 2804, 4189
BspMI ACCTGC 6 cut(s) 577, 2078, 2680, 2880, 3118, 4178
BspPI GGATC 2 cut(s) 1234, 3334
BspT107I GGYRCC 3 cut(s) 1853, 2618, 3953
BspTNI GGTCTC 2 cut(s) 489, 509
BsrDI GCAATG 3 cut(s) 1216, 2508, 3316
BsrI ACTGG 6 cut(s) 167, 1725, 2142, 2220, 3825, 4242
BssNAI GTATAC 2 cut(s) 1512, 3612
BssT1I CCWWGG 4 cut(s) 258, 2373, 3230, 4421
Bst1107I GTATAC 2 cut(s) 1512, 3612
Bst2UI CCWGG 2 cut(s) 11, 647
Bst6I CTCTTC 8 cut(s) 301, 1178, 2181, 2416, 2447, 2666, 2894, 4281
BstAPI GCANNNNNTGC 5 cut(s) 453, 696, 858, 2556, 2799
BstBAI YACGTR 2 cut(s) 629, 2732
BstDSI CCRYGG 5 cut(s) 15, 430, 2042, 2533, 4142
BstENI CCTNNNNNAGG 1 cut(s) 4425
BstFNI CGCG 2 cut(s) 432, 472
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 3 cut(s) 148, 472, 4433
BstMAI GTCTC 4 cut(s) 365, 489, 509, 1391
BstMCI CGRYCG 1 cut(s) 53
BstNI CCWGG 2 cut(s) 11, 647
BstNSI RCATGY 7 cut(s) 1005, 1937, 1961, 2261, 3575, 4037, 4361
BstPAI GACNNNNGTC 3 cut(s) 1581, 3681, 4151
BstSCI CCNGG 7 cut(s) 9, 645, 733, 768, 1466, 2871, 3566
BstSFI CTRYAG 7 cut(s) 697, 1728, 1998, 2085, 2800, 3828, 4185
BstSLI GKGCMC 4 cut(s) 248, 323, 2438, 3804
BstUI CGCG 2 cut(s) 432, 472
BstV2I GAAGAC 2 cut(s) 135, 1454
BstXI CCANNNNNNTGG 1 cut(s) 4163
BstZ17I GTATAC 2 cut(s) 1512, 3612
BstZI CGGCCG 1 cut(s) 50
Bsu15I ATCGAT 1 cut(s) 507
BsuI GTATCC 2 cut(s) 393, 2496
BsuRI GGCC 9 cut(s) 14, 34, 52, 83, 1408, 1703, 1816, 3508, 3916
BsuTUI ATCGAT 1 cut(s) 507
BtgI CCRYGG 5 cut(s) 15, 430, 2042, 2533, 4142
BtrI CACGTC 5 cut(s) 133, 265, 1918, 2380, 4018
BtsI GCAGTG 3 cut(s) 323, 1651, 3751
BveI ACCTGC 6 cut(s) 577, 2078, 2680, 2880, 3118, 4178
CaiI CAGNNNCTG 2 cut(s) 1071, 2868
CfoI GCGC 3 cut(s) 148, 472, 4433
Cfr13I GGNCC 7 cut(s) 33, 1583, 1702, 1814, 3683, 3914, 4458
Cfr42I CCGCGG 1 cut(s) 433
ClaI ATCGAT 1 cut(s) 507
CseI GACGC 1 cut(s) 3793
Csp6I GTAC 4 cut(s) 685, 2619, 2641, 2788
CviQI GTAC 4 cut(s) 685, 2619, 2641, 2788
DraIII CACNNNGTG 2 cut(s) 327, 2442
EaeI YGGCCR 1 cut(s) 50
EagI CGGCCG 1 cut(s) 50
Eam1104I CTCTTC 8 cut(s) 301, 1178, 2181, 2416, 2447, 2666, 2894, 4281
EarI CTCTTC 8 cut(s) 301, 1178, 2181, 2416, 2447, 2666, 2894, 4281
EciI GGCGGA 1 cut(s) 193
Ecl136II GAGCTC 3 cut(s) 643, 803, 2746
EclXI CGGCCG 1 cut(s) 50
Eco130I CCWWGG 4 cut(s) 258, 2373, 3230, 4421
Eco24I GRGCYC 6 cut(s) 256, 645, 805, 2347, 2371, 2748
Eco31I GGTCTC 2 cut(s) 489, 509
Eco47I GGWCC 3 cut(s) 1583, 3683, 4458
Eco52I CGGCCG 1 cut(s) 50
Eco53kI GAGCTC 3 cut(s) 643, 803, 2746
Eco57I CTGAAG 5 cut(s) 1716, 1854, 2369, 3816, 3954
Eco88I CYCGRG 1 cut(s) 36
EcoICRI GAGCTC 3 cut(s) 643, 803, 2746
EcoNI CCTNNNNNAGG 1 cut(s) 4425
EcoO109I RGGNCCY 1 cut(s) 33
EcoRI GAATTC 4 cut(s) 5, 99, 2027, 4127
EcoRII CCWGG 2 cut(s) 9, 645
EcoT14I CCWWGG 4 cut(s) 258, 2373, 3230, 4421
EcoT22I ATGCAT 2 cut(s) 1935, 4035
EcoT38I GRGCYC 6 cut(s) 256, 645, 805, 2347, 2371, 2748
ErhI CCWWGG 4 cut(s) 258, 2373, 3230, 4421
FalI AAGNNNNNCTT 4 cut(s) 1689, 1721, 3789, 3821
FaqI GGGAC 5 cut(s) 315, 717, 1295, 1877, 3977
FauI CCCGC 3 cut(s) 1254, 1321, 3354
FauNDI CATATG 1 cut(s) 2979
FbaI TGATCA 2 cut(s) 1978, 4078
FblI GTMKAC 3 cut(s) 1511, 2384, 3611
FriOI GRGCYC 6 cut(s) 256, 645, 805, 2347, 2371, 2748
FspBI CTAG 7 cut(s) 1095, 1131, 2597, 3096, 3195, 4320, 4422
GlaI GCGC 3 cut(s) 147, 471, 4432
GsaI CCCAGC 3 cut(s) 1243, 3343, 3430
GsuI CTGGAG 8 cut(s) 545, 629, 1916, 2255, 2648, 4016, 4259, 4355
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 9 cut(s) 14, 34, 52, 83, 1408, 1703, 1816, 3508, 3916
HapII CCGG 8 cut(s) 735, 769, 1468, 1873, 2872, 3568, 3674, 3973
HgaI GACGC 1 cut(s) 3793
HhaI GCGC 3 cut(s) 148, 472, 4433
Hin6I GCGC 3 cut(s) 146, 470, 4431
HinP1I GCGC 3 cut(s) 146, 470, 4431
HincII GTYRAC 1 cut(s) 2385
HindII GTYRAC 1 cut(s) 2385
HindIII AAGCTT 2 cut(s) 1646, 3746
HpaII CCGG 8 cut(s) 735, 769, 1468, 1873, 2872, 3568, 3674, 3973
Hpy99I CGWCG 2 cut(s) 113, 116
HpyAV CCTTC 7 cut(s) 212, 467, 2320, 2344, 2570, 2752, 4372
HpyCH4IV ACGT 7 cut(s) 132, 264, 628, 1917, 2379, 2731, 4017
HpySE526I ACGT 7 cut(s) 132, 264, 628, 1917, 2379, 2731, 4017
HspAI GCGC 3 cut(s) 146, 470, 4431
Kpn2I TCCGGA 2 cut(s) 1872, 3972
KpnI GGTACC 1 cut(s) 2622
Ksp22I TGATCA 2 cut(s) 1978, 4078
KspI CCGCGG 1 cut(s) 433
MaeI CTAG 7 cut(s) 1095, 1131, 2597, 3096, 3195, 4320, 4422
MaeII ACGT 7 cut(s) 132, 264, 628, 1917, 2379, 2731, 4017
MfeI CAATTG 2 cut(s) 120, 1794
MlyI GAGTC 1 cut(s) 818
MmeI TCCRAC 6 cut(s) 1117, 1404, 2228, 3217, 3504, 4328
Mph1103I ATGCAT 2 cut(s) 1935, 4035
MroI TCCGGA 2 cut(s) 1872, 3972
MroXI GAANNNNTTC 2 cut(s) 2190, 4290
MspI CCGG 8 cut(s) 735, 769, 1468, 1873, 2872, 3568, 3674, 3973
MspR9I CCNGG 7 cut(s) 11, 647, 735, 770, 1468, 2873, 3568
MunI CAATTG 2 cut(s) 120, 1794
Mva1269I GAATGC 2 cut(s) 852, 2964
MvaI CCWGG 2 cut(s) 11, 647
MvnI CGCG 2 cut(s) 432, 472
NciI CCSGG 5 cut(s) 735, 770, 1468, 2873, 3568
NdeI CATATG 1 cut(s) 2979
NmeAIII GCCGAG 1 cut(s) 109
NmuCI GTSAC 5 cut(s) 629, 668, 1112, 1787, 3887
NsiI ATGCAT 2 cut(s) 1935, 4035
NspI RCATGY 7 cut(s) 1005, 1937, 1961, 2261, 3575, 4037, 4361
OliI CACNNNNGTG 2 cut(s) 3118, 3894
PaeI GCATGC 2 cut(s) 2261, 4361
PaqCI CACCTGC 4 cut(s) 577, 2680, 2880, 3118
PciI ACATGT 1 cut(s) 1001
PctI GAATGC 2 cut(s) 852, 2964
PdmI GAANNNNTTC 2 cut(s) 2190, 4290
PflMI CCANNNNNTGG 1 cut(s) 3236
PfoI TCCNGGA 1 cut(s) 733
PleI GAGTC 1 cut(s) 817
PpsI GAGTC 1 cut(s) 817
Ppu21I YACGTR 2 cut(s) 629, 2732
PscI ACATGT 1 cut(s) 1001
PshAI GACNNNNGTC 3 cut(s) 1581, 3681, 4151
PshBI ATTAAT 1 cut(s) 3774
PsiI TTATAA 1 cut(s) 3495
Psp124BI GAGCTC 3 cut(s) 645, 805, 2748
Psp6I CCWGG 2 cut(s) 9, 645
PspFI CCCAGC 3 cut(s) 1239, 3339, 3426
PspGI CCWGG 2 cut(s) 9, 645
PspPI GGNCC 7 cut(s) 33, 1583, 1702, 1814, 3683, 3914, 4458
PstI CTGCAG 4 cut(s) 701, 2089, 2804, 4189
PstNI CAGNNNCTG 2 cut(s) 1071, 2868
PvuII CAGCTG 8 cut(s) 553, 696, 1071, 1694, 1832, 2656, 3168, 3794
RsaI GTAC 4 cut(s) 686, 2620, 2642, 2789
RsaNI GTAC 4 cut(s) 685, 2619, 2641, 2788
SacI GAGCTC 3 cut(s) 645, 805, 2748
SacII CCGCGG 1 cut(s) 433
SalI GTCGAC 1 cut(s) 2383
Sau96I GGNCC 7 cut(s) 33, 1583, 1702, 1814, 3683, 3914, 4458
SbfI CCTGCAGG 2 cut(s) 2089, 4189
ScaI AGTACT 1 cut(s) 2642
SchI GAGTC 1 cut(s) 818
ScrFI CCNGG 7 cut(s) 11, 647, 735, 770, 1468, 2873, 3568
SdaI CCTGCAGG 2 cut(s) 2089, 4189
SfcI CTRYAG 7 cut(s) 697, 1728, 1998, 2085, 2800, 3828, 4185
Sfr303I CCGCGG 1 cut(s) 433
SgrBI CCGCGG 1 cut(s) 433
SinI GGWCC 3 cut(s) 1583, 3683, 4458
SmlI CTYRAG 9 cut(s) 804, 1051, 1418, 2018, 2747, 2922, 3157, 3518, 4118
SmoI CTYRAG 9 cut(s) 804, 1051, 1418, 2018, 2747, 2922, 3157, 3518, 4118
SphI GCATGC 2 cut(s) 2261, 4361
Sse8387I CCTGCAGG 2 cut(s) 2089, 4189
SspI AATATT 2 cut(s) 1678, 3778
SspMI CTAG 7 cut(s) 1095, 1131, 2597, 3096, 3195, 4320, 4422
SstI GAGCTC 3 cut(s) 645, 805, 2748
StyD4I CCNGG 7 cut(s) 9, 645, 733, 768, 1466, 2871, 3566
StyI CCWWGG 4 cut(s) 258, 2373, 3230, 4421
TaiI ACGT 7 cut(s) 135, 267, 631, 1920, 2382, 2734, 4020
TatI WGTACW 3 cut(s) 684, 2640, 2787
TauI GCSGC 7 cut(s) 23, 52, 178, 236, 432, 435, 3919
TseFI GTSAC 5 cut(s) 629, 668, 1112, 1787, 3887
Tsp45I GTSAC 5 cut(s) 629, 668, 1112, 1787, 3887
TspGWI ACGGA 7 cut(s) 129, 388, 687, 1398, 2198, 3498, 4298
Van91I CCANNNNNTGG 1 cut(s) 3236
VneI GTGCAC 3 cut(s) 244, 319, 2434
VpaK11BI GGWCC 3 cut(s) 1583, 3683, 4458
VspI ATTAAT 1 cut(s) 3774
XagI CCTNNNNNAGG 1 cut(s) 4425
XapI RAATTY 9 cut(s) 5, 99, 1481, 1925, 2027, 3020, 3581, 4025, 4127
XbaI TCTAGA 3 cut(s) 1094, 2596, 3194
XceI RCATGY 7 cut(s) 1005, 1937, 1961, 2261, 3575, 4037, 4361
XcmI CCANNNNNNNNNTGG 4 cut(s) 1254, 1367, 3354, 3467
XmaJI CCTAGG 1 cut(s) 4421
XmiI GTMKAC 3 cut(s) 1511, 2384, 3611
XmnI GAANNNNTTC 2 cut(s) 2190, 4290
XspI CTAG 7 cut(s) 1095, 1131, 2597, 3096, 3195, 4320, 4422
ZrmI AGTACT 1 cut(s) 2642
Zsp2I ATGCAT 2 cut(s) 1935, 4035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.