RchiOBHm_Chr7g0223871

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000280
Physical Location & Seq
Forward (+)
45771955 .. 45775507
3553 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20045

Sequence Viewer

Length: 2961 bp
ATGGCGGCGAGAAAGAAAAGCAAAGGCCTCTGCCGGCCGAGGCAAGAAGAGAACGGCGCTGGTCTGGAGGAGATGAAGGCCGAGGGTATTTTGGGAATTGAGAACGAGGGAGCTAATGGGGAAGTAAAAGAAGAAGCTGTGGCTCCTTTGGTGGAGGGCAGAGGCAGGAAGCGGCGGAGGAAGAAACAAGAAGAAGACGGTGGTGGTGGTGAGGGTGTTTTGGGAAATCAAAACGAGGAAGAGGTTGCTGAAGATGGTGTGGAGGTAAAGACGCGTTATCCACAGAGGGCGACAAGGGCTGTAAGGGCGGTGGTGGAGGAGGAGGAGGAGGAGGAGCCGGAGATTCGGAAGAGGACGAAGAAGAGAAAAGCAGCGGTGGTGGAGGAGGAGGAGCCGGAGATTCGGAAGATGATGAAGAAGAGAAAAGCAGCGGCGGCGGAGGGAGAGGAAGATGAGGCAGTGAAGGACGTTTCAACAATGTGCCATCAGTGTCAGAGGAATGATAAAGGGAGAGTGGTTCGGTGCACGAGTTGTCCGAAAATGGGTAAGGGAATGAGGAAGAGGTATTGTATCCCTTGCATACAGAGGTGGTATCCCAACACATCGGAGGAAGCCATTGCTGAGGCTTGTCCTGTGTGCCGCGGCAACTGCAACTGCAAAGCCTGCCTTCGACTGGATGTGCCTAATAGGTGTTTGAAGAATCTAGACCCGAAAATCGACGAGGAGACCAAGCTGGAGCACTGTAAGTATCTGGTTCATCAGCTGCTTCCGTATTTGAAGAGGATTAGGGATGAGCAGGTGCGTGAGATGGTGGTGGAAGCCAAGAGGCAGGGATTAAGTGATGTTTCAGAGCTCAAGGTGGAGAAGGCTAATTGCGCTGACGATGAGCGTATGTACTGCAATAACTGCAGCACTTCGATTTTTGATTTTCATAGGAGTTGTCCTGATCCTGAGTGTGAGTATGATCTGTGCCTCATTTGCTGCCGGGAGATTCGAGACGGCAAGTGGAAGAAGAGAGGTATGGAGGAGGAAGACAGTCAGAAGTATGTTGATTTGGGGCGTGAGTATTTGCATGGAAAGGGAGAGTTGAAGAGCTTGCTAAAAAAAAAGAGAGAAAAGAAGTTGAAGAGCTTGCTAAAGAAGAAAAGAGAAGAAGAGTTGAAGGAGAATGTTGATGTGGGAGTGAAGCTGGACTCGTCAGAATCTGCTCAAGAGTCCTCGAGAGCAAGTGAAGATGGAAATATTCAAGATGGGCAGCAGAAAAAGAGAGGCAGAGAGGATGAAGTAAGTGAGGAATTTGTTAATGTGGGGGAGTGTTTGGATGGAGAGGGAGAGCTGAAGAGGAATGATGATGTGGGAGTGAAGCAGGACGAGGCCGAATCTGCTGAAGAGTCCACATTTGAGTGGAGAGCAAGTGAAGATGGAAGCATTCTCTGCCCTCCAACACACATGAAAGGTTGTGGTAAGCATAATTTAGAATTGAGGTGCAATTTTTCTCAGAATCATGTCATAGATTTGGTAGAGAAAGCAGGAAAAATAGATGTAAGTTACAATCCTATGCATGCTAGTAGTGAAACATGTGCACAAAAGTGTTCATGCGTTAAACCTGTGAATGATGCTGTCAACTCAAGTAGCAGCAGCTGTAAGTTAAGAAAAGCAGCCTCTAGAGACGGTTCTGATGATAACTACTTGTTCTGTCCAAGGGTCGGAGAAATCCAAGATGAGGATTTTGGGCATTTTCAGTGGCATTGGATGAGGGGTGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCAGGTTTGAGCTGGGAACCGTTAGTCATGTGGCGTGCATTTAGGCAGATAAAGAATCTCAAGCATGATAGAGAATTGGCAGTCCATACCATTGAATGCTTGGATTGGAGTTTCAAAGAAACTAATAACCTCCATTTCTTTACTGGATATTCCGAAGGCCGGCCTGATGAGAAAGGATGGCCTGAGATTCTCAAGCTCAAAGACTTCCCCCCATCTACTGAGTTTGACAATCGTCTCCCCCGGCATGGTAAGGAATTTGTCTGTTGTCTGCCTTTCAAAGAGTATACTCACCCCAACAAGGGAACCCTGAACCTTGCTATCCACTTGCCACCTACCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACGGGGAGATTCTGTGACCAAGCTTCACTGCGATATGTCTGATGCAGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACAACTGAACAACTCGCAACTATAGATAAGTTGAAAAAAAAGCACAGGAAGCAAGACGAAAGAGAGATATTTGGGAATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCTCAGGAAGAATCAGCTGAAGTATGTGAGGATGGTGGTGCCTTATGTGAGGATGGTGGTGCCTTATGGGACATTTTCCGGATCCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCACATCAATGAATTTAGGCATGTGCACTGTAATCCTTTGCAGTGTGTTACTCATGCTATACATGATCAGACTGTTTATCTCACTGTGGAGCACAAGAGGAAGCTTAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTGGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGCCAAACCATAGGGCCAGCGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTGTGAAGGAGATTTCTGGTGAGAATGCGAAGCCTGAGGATGTGAAGCCTAAGAGGAGGCGCAAGGGGAGGCGCAAGGGGAACAAAGGAGAGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

986

Amino Acids

111.96

Weight (kDa)

6.24

Isoelectric Point (pI)

53.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 157 - 224 1.2e-06 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 834 - 912 1.7e-12 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 787
Acc36I ACCTGC 2 cut(s) 787, 2669
AccB1I GGYRCC 2 cut(s) 2423, 2444
AccI GTMKAC 1 cut(s) 2081
AccII CGCG 2 cut(s) 274, 642
AccIII TCCGGA 1 cut(s) 2463
AclWI GGATC 4 cut(s) 941, 1804, 2461, 2474
AcoI YGGCCR 1 cut(s) 35
AcsI RAATTY 4 cut(s) 1295, 2051, 2516, 2618
AcuI CTGAAG 5 cut(s) 270, 1358, 1407, 2286, 2424
AfaI GTAC 1 cut(s) 896
AflII CTTAAG 1 cut(s) 2609
AflIII ACRYGT 2 cut(s) 272, 1577
AjnI CCWGG 1 cut(s) 2469
AleI CACNNNNGTG 2 cut(s) 1588, 2364
Alw21I GWGCWC 6 cut(s) 527, 741, 855, 1585, 2532, 2598
Alw26I GTCTC 4 cut(s) 719, 990, 1662, 2036
Alw44I GTGCAC 3 cut(s) 523, 1581, 2528
AlwI GGATC 4 cut(s) 941, 1804, 2461, 2474
AlwNI CAGNNNCTG 2 cut(s) 1205, 1641
Ama87I CYCGRG 1 cut(s) 1219
Aor13HI TCCGGA 1 cut(s) 2463
ApaLI GTGCAC 3 cut(s) 523, 1581, 2528
ApoI RAATTY 4 cut(s) 1295, 2051, 2516, 2618
Asp700I GAANNNNTTC 1 cut(s) 2781
AspLEI GCGC 4 cut(s) 59, 878, 2925, 2937
AspS9I GGNCC 4 cut(s) 2153, 2272, 2384, 2808
AsuC2I CCSGG 2 cut(s) 986, 2038
AsuHPI GGTGA 7 cut(s) 221, 1772, 2078, 2707, 2768, 2842, 2894
AvaI CYCGRG 1 cut(s) 1219
AvaII GGWCC 1 cut(s) 2153
AxyI CCTNAGG 2 cut(s) 2388, 2898
BaeGI GKGCMC 3 cut(s) 527, 1585, 2532
BamHI GGATCC 1 cut(s) 2466
BanI GGYRCC 2 cut(s) 2423, 2444
BanII GRGCYC 1 cut(s) 855
BauI CACGAG 1 cut(s) 526
BbsI GAAGAC 2 cut(s) 201, 1038
Bbv12I GWGCWC 6 cut(s) 527, 741, 855, 1585, 2532, 2598
BbvCI CCTCAGC 1 cut(s) 621
BceAI ACGGC 3 cut(s) 70, 1015, 2617
BciT130I CCWGG 1 cut(s) 2471
BciVI GTATCC 2 cut(s) 581, 603
BclI TGATCA 1 cut(s) 2569
BcnI CCSGG 2 cut(s) 986, 2038
BcoDI GTCTC 4 cut(s) 719, 990, 1662, 2036
BfaI CTAG 3 cut(s) 704, 1566, 1665
BfmI CTRYAG 3 cut(s) 907, 2298, 2676
BfoI RGCGCY 1 cut(s) 60
BfrI CTTAAG 1 cut(s) 2609
BfuAI ACCTGC 2 cut(s) 787, 2669
BfuI GTATCC 2 cut(s) 581, 603
Bme1390I CCNGG 3 cut(s) 986, 2038, 2471
Bme18I GGWCC 1 cut(s) 2153
BmeT110I CYCGRG 1 cut(s) 1219
BmgT120I GGNCC 4 cut(s) 2153, 2272, 2384, 2808
BmiI GGNNCC 9 cut(s) 144, 336, 393, 1815, 2101, 2154, 2425, 2446, 2468
BmrFI CCNGG 3 cut(s) 986, 2038, 2471
BmsI GCATC 3 cut(s) 1606, 2227, 2650
BoxI GACNNNNGTC 3 cut(s) 2028, 2151, 2642
BpiI GAAGAC 2 cut(s) 201, 1038
BpmI CTGGAG 4 cut(s) 86, 755, 2507, 2846
Bpu10I CCTNAGC 1 cut(s) 621
BpuEI CTTGAG 5 cut(s) 839, 1194, 1612, 1841, 1973
BpuMI CCSGG 2 cut(s) 986, 2038
BsaI GGTCTC 1 cut(s) 719
BsaJI CCNNGG 6 cut(s) 38, 81, 640, 1700, 2036, 2633
BsaWI WCCGGW 1 cut(s) 2463
BsaXI ACNNNNNCTCC 6 cut(s) 1399, 1429, 2193, 2223, 2649, 2679
Bse118I RCCGGY 2 cut(s) 33, 1956
Bse1I ACTGG 3 cut(s) 678, 1945, 2733
Bse21I CCTNAGG 2 cut(s) 2388, 2898
Bse3DI GCAATG 2 cut(s) 615, 1786
BseAI TCCGGA 1 cut(s) 2463
BseBI CCWGG 1 cut(s) 2471
BseDI CCNNGG 6 cut(s) 38, 81, 640, 1700, 2036, 2633
BseMI GCAATG 2 cut(s) 615, 1786
BseMII CTCAG 9 cut(s) 612, 942, 1511, 1971, 2007, 2148, 2402, 2736, 2889
BseNI ACTGG 3 cut(s) 678, 1945, 2733
BseSI GKGCMC 3 cut(s) 527, 1585, 2532
BseX3I CGGCCG 1 cut(s) 35
BseYI CCCAGC 1 cut(s) 1809
Bsh1236I CGCG 2 cut(s) 274, 642
Bsh1285I CGRYCG 1 cut(s) 38
BshNI GGYRCC 2 cut(s) 2423, 2444
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 6 cut(s) 527, 741, 855, 1585, 2532, 2598
BsiHKCI CYCGRG 1 cut(s) 1219
BsiSI CCGG 8 cut(s) 34, 338, 395, 985, 1957, 2038, 2144, 2464
BslFI GGGAC 1 cut(s) 2468
BsmAI GTCTC 4 cut(s) 719, 990, 1662, 2036
BsmBI CGTCTC 3 cut(s) 990, 1662, 2036
BsmFI GGGAC 1 cut(s) 2468
BsmI GAATGC 3 cut(s) 1428, 1898, 2893
Bso31I GGTCTC 1 cut(s) 719
BsoBI CYCGRG 1 cut(s) 1219
Bsp1286I GDGCHC 6 cut(s) 527, 741, 855, 1585, 2532, 2598
Bsp13I TCCGGA 1 cut(s) 2463
Bsp143I GATC 6 cut(s) 946, 964, 1770, 1796, 2466, 2569
BspCNI CTCAG 9 cut(s) 613, 943, 1510, 1972, 2008, 2147, 2401, 2737, 2890
BspEI TCCGGA 1 cut(s) 2463
BspFNI CGCG 2 cut(s) 274, 642
BspLI GGNNCC 9 cut(s) 144, 336, 393, 1815, 2101, 2154, 2425, 2446, 2468
BspMAI CTGCAG 2 cut(s) 911, 2680
BspMI ACCTGC 2 cut(s) 787, 2669
BspPI GGATC 4 cut(s) 941, 1804, 2461, 2474
BspQI GCTCTTC 2 cut(s) 1085, 1121
BspT107I GGYRCC 2 cut(s) 2423, 2444
BspTI CTTAAG 1 cut(s) 2609
BspTNI GGTCTC 1 cut(s) 719
BsrDI GCAATG 2 cut(s) 615, 1786
BsrFI RCCGGY 2 cut(s) 33, 1956
BsrI ACTGG 3 cut(s) 678, 1945, 2733
BssAI RCCGGY 2 cut(s) 33, 1956
BssECI CCNNGG 6 cut(s) 38, 81, 640, 1700, 2036, 2633
BssMI GATC 6 cut(s) 946, 964, 1770, 1796, 2466, 2569
BssNAI GTATAC 1 cut(s) 2082
BssSI CACGAG 1 cut(s) 526
BssT1I CCWWGG 1 cut(s) 1700
Bst1107I GTATAC 1 cut(s) 2082
Bst2BI CACGAG 1 cut(s) 526
Bst2UI CCWGG 1 cut(s) 2471
Bst4CI ACNGT 8 cut(s) 200, 743, 1037, 1673, 1818, 2534, 2578, 2590
BstAFI CTTAAG 1 cut(s) 2609
BstAPI GCANNNNNTGC 3 cut(s) 663, 906, 1434
BstC8I GCNNGC 9 cut(s) 35, 664, 1097, 1133, 1563, 1833, 1958, 2812, 2850
BstDSI CCRYGG 2 cut(s) 640, 2633
BstFNI CGCG 2 cut(s) 274, 642
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 4 cut(s) 59, 878, 2925, 2937
BstKTI GATC 6 cut(s) 949, 967, 1773, 1799, 2469, 2572
BstMAI GTCTC 4 cut(s) 719, 990, 1662, 2036
BstMBI GATC 6 cut(s) 946, 964, 1770, 1796, 2466, 2569
BstMCI CGRYCG 1 cut(s) 38
BstNI CCWGG 1 cut(s) 2471
BstNSI RCATGY 4 cut(s) 1565, 1581, 2528, 2852
BstPAI GACNNNNGTC 3 cut(s) 2028, 2151, 2642
BstSCI CCNGG 3 cut(s) 984, 2036, 2469
BstSFI CTRYAG 3 cut(s) 907, 2298, 2676
BstSLI GKGCMC 3 cut(s) 527, 1585, 2532
BstUI CGCG 2 cut(s) 274, 642
BstV2I GAAGAC 2 cut(s) 201, 1038
BstX2I RGATCY 1 cut(s) 2466
BstXI CCANNNNNNTGG 1 cut(s) 2654
BstYI RGATCY 1 cut(s) 2466
BstZ17I GTATAC 1 cut(s) 2082
BstZI CGGCCG 1 cut(s) 35
Bsu36I CCTNAGG 2 cut(s) 2388, 2898
BsuI GTATCC 2 cut(s) 581, 603
BtgI CCRYGG 2 cut(s) 640, 2633
BtsI GCAGTG 3 cut(s) 465, 2221, 2552
BtsIMutI CAGTG 9 cut(s) 465, 494, 739, 1748, 2221, 2530, 2552, 2586, 2726
BveI ACCTGC 2 cut(s) 787, 2669
Cac8I GCNNGC 9 cut(s) 35, 664, 1097, 1133, 1563, 1833, 1958, 2812, 2850
CaiI CAGNNNCTG 2 cut(s) 1205, 1641
CfoI GCGC 4 cut(s) 59, 878, 2925, 2937
Cfr10I RCCGGY 2 cut(s) 33, 1956
Cfr13I GGNCC 4 cut(s) 2153, 2272, 2384, 2808
Cfr42I CCGCGG 1 cut(s) 643
CseI GACGC 1 cut(s) 280
Csp6I GTAC 1 cut(s) 895
CviQI GTAC 1 cut(s) 895
DpnI GATC 6 cut(s) 948, 966, 1772, 1798, 2468, 2571
DpnII GATC 6 cut(s) 946, 964, 1770, 1796, 2466, 2569
EaeI YGGCCR 1 cut(s) 35
EagI CGGCCG 1 cut(s) 35
EciI GGCGGA 3 cut(s) 190, 452, 2784
Ecl136II GAGCTC 1 cut(s) 853
EclXI CGGCCG 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 1700
Eco147I AGGCCT 1 cut(s) 27
Eco24I GRGCYC 1 cut(s) 855
Eco31I GGTCTC 1 cut(s) 719
Eco47I GGWCC 1 cut(s) 2153
Eco52I CGGCCG 1 cut(s) 35
Eco53kI GAGCTC 1 cut(s) 853
Eco57I CTGAAG 5 cut(s) 270, 1358, 1407, 2286, 2424
Eco81I CCTNAGG 2 cut(s) 2388, 2898
Eco88I CYCGRG 1 cut(s) 1219
EcoICRI GAGCTC 1 cut(s) 853
EcoRI GAATTC 1 cut(s) 2618
EcoRII CCWGG 1 cut(s) 2469
EcoT14I CCWWGG 1 cut(s) 1700
EcoT22I ATGCAT 1 cut(s) 1563
EcoT38I GRGCYC 1 cut(s) 855
ErhI CCWWGG 1 cut(s) 1700
Esp3I CGTCTC 3 cut(s) 990, 1662, 2036
FalI AAGNNNNNCTT 4 cut(s) 651, 683, 2259, 2291
FaqI GGGAC 1 cut(s) 2468
FbaI TGATCA 1 cut(s) 2569
FblI GTMKAC 1 cut(s) 2081
FriOI GRGCYC 1 cut(s) 855
FseI GGCCGGCC 1 cut(s) 1960
FspBI CTAG 3 cut(s) 704, 1566, 1665
GlaI GCGC 4 cut(s) 58, 877, 2924, 2936
GsaI CCCAGC 1 cut(s) 1813
GsuI CTGGAG 4 cut(s) 86, 755, 2507, 2846
HaeII RGCGCY 1 cut(s) 60
HapII CCGG 8 cut(s) 34, 338, 395, 985, 1957, 2038, 2144, 2464
HgaI GACGC 1 cut(s) 280
HhaI GCGC 4 cut(s) 59, 878, 2925, 2937
Hin6I GCGC 4 cut(s) 57, 876, 2923, 2935
HinP1I GCGC 4 cut(s) 57, 876, 2923, 2935
HincII GTYRAC 1 cut(s) 1624
HindII GTYRAC 1 cut(s) 1624
HindIII AAGCTT 2 cut(s) 2216, 2606
HpaII CCGG 8 cut(s) 34, 338, 395, 985, 1957, 2038, 2144, 2464
HphI GGTGA 7 cut(s) 221, 1772, 2078, 2707, 2768, 2842, 2894
Hpy166II GTNNAC 7 cut(s) 525, 1395, 1583, 1624, 2082, 2530, 2637
Hpy8I GTNNAC 7 cut(s) 525, 1395, 1583, 1624, 2082, 2530, 2637
Hpy99I CGWCG 1 cut(s) 722
HpyAV CCTTC 7 cut(s) 70, 457, 677, 859, 1156, 1946, 2863
HpyCH4III ACNGT 8 cut(s) 200, 743, 1037, 1673, 1818, 2534, 2578, 2590
HpyCH4IV ACGT 1 cut(s) 468
HpySE526I ACGT 1 cut(s) 468
HspAI GCGC 4 cut(s) 57, 876, 2923, 2935
Kpn2I TCCGGA 1 cut(s) 2463
KroI GCCGGC 2 cut(s) 33, 1956
KroNI GCCGGC 2 cut(s) 35, 1958
Ksp22I TGATCA 1 cut(s) 2569
KspI CCGCGG 1 cut(s) 643
Kzo9I GATC 6 cut(s) 946, 964, 1770, 1796, 2466, 2569
LguI GCTCTTC 2 cut(s) 1085, 1121
LmnI GCTCC 7 cut(s) 110, 148, 334, 391, 736, 2188, 2593
LweI GCATC 3 cut(s) 1606, 2227, 2650
MaeI CTAG 3 cut(s) 704, 1566, 1665
MaeII ACGT 1 cut(s) 468
MaeIII GTNAC 4 cut(s) 1547, 2209, 2357, 2551
MalI GATC 6 cut(s) 948, 966, 1772, 1798, 2468, 2571
MboI GATC 6 cut(s) 946, 964, 1770, 1796, 2466, 2569
MflI RGATCY 1 cut(s) 2466
MhlI GDGCHC 6 cut(s) 527, 741, 855, 1585, 2532, 2598
MluI ACGCGT 1 cut(s) 272
MlyI GAGTC 3 cut(s) 1187, 1223, 1400
MmeI TCCRAC 2 cut(s) 1466, 1687
Mph1103I ATGCAT 1 cut(s) 1563
MroI TCCGGA 1 cut(s) 2463
MroNI GCCGGC 2 cut(s) 33, 1956
MroXI GAANNNNTTC 1 cut(s) 2781
MseI TTAA 6 cut(s) 836, 1302, 1602, 1649, 2244, 2610
MslI CAYNNNNRTG 4 cut(s) 1402, 1588, 2364, 2511
MspA1I CMGCKG 8 cut(s) 374, 431, 642, 763, 1641, 2264, 2402, 2485
MspCI CTTAAG 1 cut(s) 2609
MspI CCGG 8 cut(s) 34, 338, 395, 985, 1957, 2038, 2144, 2464
MspR9I CCNGG 3 cut(s) 986, 2038, 2471
Mva1269I GAATGC 3 cut(s) 1428, 1898, 2893
MvaI CCWGG 1 cut(s) 2471
MvnI CGCG 2 cut(s) 274, 642
NaeI GCCGGC 2 cut(s) 35, 1958
NciI CCSGG 2 cut(s) 986, 2038
NdeII GATC 6 cut(s) 946, 964, 1770, 1796, 2466, 2569
NgoMIV GCCGGC 2 cut(s) 33, 1956
NlaIV GGNNCC 9 cut(s) 144, 336, 393, 1815, 2101, 2154, 2425, 2446, 2468
NmeAIII GCCGAG 2 cut(s) 63, 106
NmuCI GTSAC 2 cut(s) 2209, 2357
NsiI ATGCAT 1 cut(s) 1563
NspI RCATGY 4 cut(s) 1565, 1581, 2528, 2852
OliI CACNNNNGTG 2 cut(s) 1588, 2364
PaeI GCATGC 2 cut(s) 1565, 2852
PaeR7I CTCGAG 1 cut(s) 1219
PaqCI CACCTGC 1 cut(s) 787
PceI AGGCCT 1 cut(s) 27
PciI ACATGT 1 cut(s) 1577
PciSI GCTCTTC 2 cut(s) 1085, 1121
PcsI WCGNNNNNNNCGW 1 cut(s) 533
PctI GAATGC 3 cut(s) 1428, 1898, 2893
PdiI GCCGGC 2 cut(s) 35, 1958
PdmI GAANNNNTTC 1 cut(s) 2781
PfoI TCCNGGA 1 cut(s) 2469
PleI GAGTC 3 cut(s) 1187, 1222, 1399
PpsI GAGTC 3 cut(s) 1187, 1222, 1399
PscI ACATGT 1 cut(s) 1577
PshAI GACNNNNGTC 3 cut(s) 2028, 2151, 2642
Psp124BI GAGCTC 1 cut(s) 855
Psp6I CCWGG 1 cut(s) 2469
PspFI CCCAGC 1 cut(s) 1809
PspGI CCWGG 1 cut(s) 2469
PspN4I GGNNCC 9 cut(s) 144, 336, 393, 1815, 2101, 2154, 2425, 2446, 2468
PspPI GGNCC 4 cut(s) 2153, 2272, 2384, 2808
PstI CTGCAG 2 cut(s) 911, 2680
PstNI CAGNNNCTG 2 cut(s) 1205, 1641
PsuI RGATCY 1 cut(s) 2466
PvuII CAGCTG 5 cut(s) 763, 1641, 2264, 2402, 2485
RigI GGCCGGCC 1 cut(s) 1960
RsaI GTAC 1 cut(s) 896
RsaNI GTAC 1 cut(s) 895
RseI CAYNNNNRTG 4 cut(s) 1402, 1588, 2364, 2511
SacI GAGCTC 1 cut(s) 855
SacII CCGCGG 1 cut(s) 643
SapI GCTCTTC 2 cut(s) 1085, 1121
SaqAI TTAA 6 cut(s) 836, 1302, 1602, 1649, 2244, 2610
Sau3AI GATC 6 cut(s) 946, 964, 1770, 1796, 2466, 2569
Sau96I GGNCC 4 cut(s) 2153, 2272, 2384, 2808
SbfI CCTGCAGG 1 cut(s) 2680
SchI GAGTC 3 cut(s) 1187, 1223, 1400
ScrFI CCNGG 3 cut(s) 986, 2038, 2471
SdaI CCTGCAGG 1 cut(s) 2680
SduI GDGCHC 6 cut(s) 527, 741, 855, 1585, 2532, 2598
SfaNI GCATC 3 cut(s) 1606, 2227, 2650
SfcI CTRYAG 3 cut(s) 907, 2298, 2676
Sfr274I CTCGAG 1 cut(s) 1219
Sfr303I CCGCGG 1 cut(s) 643
SgrBI CCGCGG 1 cut(s) 643
SinI GGWCC 1 cut(s) 2153
SlaI CTCGAG 1 cut(s) 1219
SmiMI CAYNNNNRTG 4 cut(s) 1402, 1588, 2364, 2511
SmlI CTYRAG 7 cut(s) 854, 1209, 1219, 1627, 1856, 1988, 2609
SmoI CTYRAG 7 cut(s) 854, 1209, 1219, 1627, 1856, 1988, 2609
SphI GCATGC 2 cut(s) 1565, 2852
Sse8387I CCTGCAGG 1 cut(s) 2680
SseBI AGGCCT 1 cut(s) 27
SspI AATATT 2 cut(s) 1243, 2248
SspMI CTAG 3 cut(s) 704, 1566, 1665
SstI GAGCTC 1 cut(s) 855
StuI AGGCCT 1 cut(s) 27
StyD4I CCNGG 3 cut(s) 984, 2036, 2469
StyI CCWWGG 1 cut(s) 1700
TaaI ACNGT 8 cut(s) 200, 743, 1037, 1673, 1818, 2534, 2578, 2590
TaiI ACGT 1 cut(s) 471
TaqI TCGA 6 cut(s) 670, 717, 917, 994, 1220, 2628
TatI WGTACW 1 cut(s) 894
TauI GCSGC 6 cut(s) 8, 175, 434, 437, 642, 645
Tru1I TTAA 6 cut(s) 836, 1302, 1602, 1649, 2244, 2610
Tru9I TTAA 6 cut(s) 836, 1302, 1602, 1649, 2244, 2610
TscAI CASTG 9 cut(s) 465, 494, 746, 1748, 2228, 2537, 2552, 2593, 2733
TseFI GTSAC 2 cut(s) 2209, 2357
Tsp45I GTSAC 2 cut(s) 2209, 2357
TspDTI ATGAA 9 cut(s) 89, 428, 746, 920, 1296, 1466, 1584, 2529, 2659
TspGWI ACGGA 2 cut(s) 759, 2789
TspRI CASTG 9 cut(s) 465, 494, 746, 1748, 2228, 2537, 2552, 2593, 2733
Vha464I CTTAAG 1 cut(s) 2609
VneI GTGCAC 3 cut(s) 523, 1581, 2528
VpaK11BI GGWCC 1 cut(s) 2153
XapI RAATTY 4 cut(s) 1295, 2051, 2516, 2618
XbaI TCTAGA 2 cut(s) 703, 1664
XceI RCATGY 4 cut(s) 1565, 1581, 2528, 2852
XcmI CCANNNNNNNNNTGG 2 cut(s) 1894, 1937
XhoI CTCGAG 1 cut(s) 1219
XmiI GTMKAC 1 cut(s) 2081
XmnI GAANNNNTTC 1 cut(s) 2781
XspI CTAG 3 cut(s) 704, 1566, 1665
Zsp2I ATGCAT 1 cut(s) 1563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.