Rmu_sc0003363.1_g000077

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003363.1
OG0000280
Physical Location & Seq
Reverse (-)
394063 .. 402754
8692 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003363.1_g000077.1.cds

Sequence Viewer

Length: 3390 bp
atggcagggtttgggggtgagggcattttgggaaatcaaaattataggaccaaacgggtgggaggtgaagggagttgggattctccgagcttgaggaagaggaagagagaaggtgaagctggagggtcatgttctaagaagaaatgtactaagggctcaaattggtttaattggaagtcctcaatgtgccatcagtgtcagaggaatgatagggccagagttgttaactgcacaagttgtaagcggaagcgattttgtgtatcatgcattcagaattggtatccccagatgtcagaggaggcaattgctgatgcttgtcctgtatgccttggaaattgcaattgcaaagcgtgcttgcgtttagatgaaactaatcataatttgaatagcaagcttgcattgcacgttagtaaagatgaagaagtggagcactctaagtatttgcttgaagcattgcttccattcttgaaaagacttaatgatgaacaagttatagagatggtgatggaggctagaagacaagggaaatctctttcggagctaaagatccaaaggtcggacagctctcctgatgatcgtgtatactgcgacaactgcaatacatctattattgatttccacagaagctgtcctatatgttcatatgacctttgtcttatctgctgccgggagattcgagatggaaaccagcaaggaagtgcagaagaagtgattggggagtatatcaatcaaggtctagactatttacatggtggaaagggcaacttaataaaaaagccttctgaaactaaactcaagtgtcctgtaaggtcaacatctaagtggaaagtaagcaaagatcgaagtattttttgcccctcaaaagacatggacggctgtggttgtagtgttctagaactgagatgcatattttctgaaaaccacatcatagagttggtagaaaaagcagaagaaattgcagaaactaaaaaactgatgtatgcagctggaccttctgcacagaagttttcatggttcaactctgcaggtggtgctgactcaagaggcaatgaattgacgaaagcagcatctcgagaggattcggaggacaactacttgtacagtccaagggctataaatatccaacatgaagactttaagcattttcgctgtcattggatccagggtgagcctgtgattgtggggaatgtgcttgaaactgcaacaggtttgagctgggaaccatttgtcatgtggcgtgcttgtcgtcagatgagaaataccgggcatgatagacatgtggaatttaaggccattgattgcttggattggtgtgagctagatatcggtatccaccaattttttactggatatttggagggccagtttgatgggaaaatgtggcctcgtattctgaaactcaacgatcagtcccttgatgattatttggagaagcgccttcctcgtcattgtgctgagtttatttgttgcttgccatttaaggaatatacccatcctcgcagtggcctcctgaaccttgctgtaaaattgcctatggagtgtgtaaagccagacatggggccaaaaatggatattgcttatggtgtgtcccaggagcttggacgtggagattcagtaactaagcttcactgtgattcatgtgatgtggtgaatgttttgacgcatactgctgaagtgacgattactgctgagcaactttgtactatagaagggttgaaaaaagatcacattgaacaagaccgaagggaaatgtttgaaaattatgagactgtggatgacaatgttgatcataaggagtctggcagtgggtcttgtgtcaggactgcaaagggtaaacaatgttgtactcaagttaagaatcaaaacagggttgcaatttttcaagaaaagactaaagctcaaactgatagttggaatggtggaattttttcaaaggaatccatgtctgaaaagaatgcagagggaggtgctttatgggacatttttcgacgacaggatgttcctaagctgcaggaatatctatggaaacactacaaggaattcaggcacacctactgttgtccattgcagcaggtcactcaccccatacatgatcagactttttatctgacattagagcacaagaggaagctgaaggaagaatatggaattgaaccatggacatttatccagaaacttggagatgctgtctttattcctgcaggatgtccacaccaagttagaaacttgaagtcgtgcattaaagtttctgttgaatttgtctcccctgaaaatgttggtgagtgcttacgtctgacagatgagttccgtacacttccacgaaagcatatagctaaagaggataaattggaggtgaagaaaatgattgttcatgctgtacatgaggctgtggagattttggatcagagtttgaggttatgtagcgttaaaaacttcagggcagagaagccttcacagccccctgttaatatatataagggcaacattgcaaacaaattgagagagaaagcagctagaggacgaccacaagaagaatcgaaagaggttcaaattcttgaggagcagccttcacaattatcacagcctatagagcctgctattgatgttggtagatttggacgcacacggagagaggaaaaagatggagggcaaccacaagaagcaagtagagtagatgaagaggtccaaattcttaagggacagccttcacagtcagcacaacatgtacagcccactgcttatgttgatagcaagcatacgatgagaaaggaaacagagggaggggtactgcaagaaacacatggagaagttgaagacgtccaaattcttgagaagcagccttggcgatcatcacaaactgtacaagccactcttcatgttggtagcattgaacacgcatctagagaagaaaaagctggatggcaaccacaagaaacaagtggagaagatgaagaagtccaaattcttgaggagcagcattggcaatcatcaaaatctgtacaagcaactgttcacattggtagcattggacatgcaatgagagaggaaaaagtgggaggacgacagcatgaaggaaggggagaacaggaagaggtccgaaatcttggtgaaaagccttcaaagttatcacaacccgaacctgtccaacccactgttgatgttgataaagcggtgcagcaactggatgagctcgtggagctccttagacattttgaacagaggaggataccgatgggaggcagagatggaagaatcagttctcaattccagcttttatgctcggagttcttgactttatctttgcatcttggttatgcacctaatgataccaaacctcacactacaccatctgctcagccgctacatgatcctccagttgtagatctttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1129

Amino Acids

128.32

Weight (kDa)

6.26

Isoelectric Point (pI)

56.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1016
AatII GACGTC 1 cut(s) 2820
Acc36I ACCTGC 2 cut(s) 1016, 2062
AccI GTMKAC 1 cut(s) 582
AciI CCGC 3 cut(s) 244, 3170, 3359
AclWI GGATC 5 cut(s) 541, 1153, 1166, 2409, 3362
AcsI RAATTY 8 cut(s) 1283, 1923, 2039, 2255, 2550, 2688, 2823, 2961
AcuI CTGAAG 3 cut(s) 1692, 2153, 2419
AcyI GRCGYC 1 cut(s) 2817
AfiI CCNNNNNNNGG 4 cut(s) 556, 1502, 1556, 3236
AflII CTTAAG 1 cut(s) 2693
AflIII ACRYGT 2 cut(s) 1276, 2722
AjiI CACGTC 1 cut(s) 1604
AjnI CCWGG 2 cut(s) 1161, 1590
AjuI GAANNNNNNNTTGG 2 cut(s) 696, 728
Alw21I GWGCWC 4 cut(s) 432, 2121, 3192, 3201
Alw26I GTCTC 2 cut(s) 1760, 2266
AlwI GGATC 5 cut(s) 541, 1153, 1166, 2409, 3362
AlwNI CAGNNNCTG 2 cut(s) 627, 3181
Ama87I CYCGRG 1 cut(s) 1071
AoxI GGCC 6 cut(s) 213, 1290, 1360, 1382, 1504, 1559
ApoI RAATTY 8 cut(s) 1283, 1923, 2039, 2255, 2550, 2688, 2823, 2961
ArsI GACNNNNNNTTYG 2 cut(s) 3283, 3315
Asp700I GAANNNNTTC 2 cut(s) 1927, 3256
AspLEI GCGC 1 cut(s) 1437
AspS9I GGNCC 7 cut(s) 48, 213, 989, 1360, 1559, 2683, 3094
AsuC2I CCSGG 2 cut(s) 668, 1264
AvaI CYCGRG 1 cut(s) 1071
AvaII GGWCC 4 cut(s) 48, 989, 2683, 3094
BamHI GGATCC 1 cut(s) 1158
BanII GRGCYC 3 cut(s) 158, 3192, 3201
BarI GAAGNNNNNNTAC 2 cut(s) 1608, 1640
BauI CACGAG 1 cut(s) 3191
BbsI GAAGAC 3 cut(s) 523, 1137, 2820
Bbv12I GWGCWC 4 cut(s) 432, 2121, 3192, 3201
BceAI ACGGC 1 cut(s) 889
BciT130I CCWGG 2 cut(s) 1163, 1592
BciVI GTATCC 3 cut(s) 291, 1340, 3219
BclI TGATCA 2 cut(s) 1786, 2092
BcnI CCSGG 2 cut(s) 668, 1264
BcoDI GTCTC 2 cut(s) 1760, 2266
BfaI CTAG 6 cut(s) 513, 737, 893, 1319, 2514, 2901
BfmI CTRYAG 5 cut(s) 1023, 1704, 2009, 2199, 2586
BfoI RGCGCY 1 cut(s) 1438
BfrI CTTAAG 1 cut(s) 2693
BfuAI ACCTGC 2 cut(s) 1016, 2062
BfuI GTATCC 3 cut(s) 291, 1340, 3219
BglII AGATCT 1 cut(s) 3382
BlpI GCTNAGC 2 cut(s) 1689, 3354
Bme1390I CCNGG 4 cut(s) 668, 1163, 1264, 1592
Bme18I GGWCC 4 cut(s) 48, 989, 2683, 3094
BmeT110I CYCGRG 1 cut(s) 1071
BmgBI CACGTC 1 cut(s) 1604
BmgT120I GGNCC 7 cut(s) 48, 213, 989, 1360, 1559, 2683, 3094
BmiI GGNNCC 3 cut(s) 1160, 1221, 1560
BmrFI CCNGG 4 cut(s) 668, 1163, 1264, 1592
BmsI GCATC 6 cut(s) 301, 893, 1076, 2173, 2906, 3313
BoxI GACNNNNGTC 1 cut(s) 651
BpiI GAAGAC 3 cut(s) 523, 1137, 2820
BpmI CTGGAG 2 cut(s) 141, 3357
Bpu10I CCTNAGC 1 cut(s) 2004
Bpu1102I GCTNAGC 2 cut(s) 1689, 3354
BpuEI CTTGAG 7 cut(s) 112, 779, 1024, 1833, 2576, 2849, 2987
BpuMI CCSGG 2 cut(s) 668, 1264
BsaHI GRCGYC 1 cut(s) 2817
BsaJI CCNNGG 6 cut(s) 328, 1106, 1162, 1590, 2156, 2840
BsaXI ACNNNNNCTCC 6 cut(s) 1349, 1379, 1599, 1629, 2172, 2202
Bsc4I CCNNNNNNNGG 4 cut(s) 556, 1502, 1556, 3236
Bse1I ACTGG 4 cut(s) 1351, 1363, 3186, 3374
Bse3DI GCAATG 6 cut(s) 398, 452, 1054, 2063, 2484, 3042
BseBI CCWGG 2 cut(s) 1163, 1592
BseDI CCNNGG 6 cut(s) 328, 1106, 1162, 1590, 2156, 2840
BseGI GGATG 6 cut(s) 1492, 1780, 2002, 2210, 2924, 3190
BseLI CCNNNNNNNGG 4 cut(s) 556, 1502, 1556, 3236
BseMI GCAATG 6 cut(s) 398, 452, 1054, 2063, 2484, 3042
BseMII CTCAG 4 cut(s) 890, 1446, 1680, 3368
BseNI ACTGG 4 cut(s) 1351, 1363, 3186, 3374
BseRI GAGGAG 4 cut(s) 311, 2573, 2984, 3235
BseYI CCCAGC 1 cut(s) 1215
BsgI GTGCAG 4 cut(s) 214, 720, 981, 3194
BshFI GGCC 6 cut(s) 215, 1292, 1362, 1384, 1506, 1561
BsiHKAI GWGCWC 4 cut(s) 432, 2121, 3192, 3201
BsiHKCI CYCGRG 1 cut(s) 1071
BsiSI CCGG 2 cut(s) 667, 1263
BslFI GGGAC 4 cut(s) 1396, 1573, 1991, 2712
BslI CCNNNNNNNGG 4 cut(s) 556, 1502, 1556, 3236
BsmAI GTCTC 2 cut(s) 1760, 2266
BsmFI GGGAC 4 cut(s) 1396, 1573, 1991, 2712
BsmI GAATGC 2 cut(s) 267, 1960
BsnI GGCC 6 cut(s) 215, 1292, 1362, 1384, 1506, 1561
BsoBI CYCGRG 1 cut(s) 1071
Bsp1286I GDGCHC 5 cut(s) 158, 432, 2121, 3192, 3201
Bsp1407I TGTACA 5 cut(s) 1098, 2377, 2725, 2860, 2998
Bsp1720I GCTNAGC 2 cut(s) 1689, 3354
Bsp19I CCATGG 1 cut(s) 2156
BspACI CCGC 3 cut(s) 244, 3170, 3359
BspANI GGCC 6 cut(s) 215, 1292, 1362, 1384, 1506, 1561
BspCNI CTCAG 4 cut(s) 891, 1447, 1681, 3367
BspLI GGNNCC 3 cut(s) 1160, 1221, 1560
BspMAI CTGCAG 3 cut(s) 1027, 2013, 2203
BspMI ACCTGC 2 cut(s) 1016, 2062
BspPI GGATC 5 cut(s) 541, 1153, 1166, 2409, 3362
BspTI CTTAAG 1 cut(s) 2693
BsrDI GCAATG 6 cut(s) 398, 452, 1054, 2063, 2484, 3042
BsrGI TGTACA 5 cut(s) 1098, 2377, 2725, 2860, 2998
BsrI ACTGG 4 cut(s) 1351, 1363, 3186, 3374
BssECI CCNNGG 6 cut(s) 328, 1106, 1162, 1590, 2156, 2840
BssNAI GTATAC 1 cut(s) 583
BssNI GRCGYC 1 cut(s) 2817
BssSI CACGAG 1 cut(s) 3191
BssT1I CCWWGG 4 cut(s) 328, 1106, 2156, 2840
Bst1107I GTATAC 1 cut(s) 583
Bst2BI CACGAG 1 cut(s) 3191
Bst2UI CCWGG 2 cut(s) 1163, 1592
Bst4CI ACNGT 8 cut(s) 1103, 1631, 1771, 2057, 2712, 2860, 3010, 3154
Bst6I CTCTTC 5 cut(s) 92, 98, 2673, 2877, 3084
BstACI GRCGYC 1 cut(s) 2817
BstAFI CTTAAG 1 cut(s) 2693
BstAPI GCANNNNNTGC 2 cut(s) 351, 1031
BstAUI TGTACA 5 cut(s) 1098, 2377, 2725, 2860, 2998
BstC8I GCNNGC 8 cut(s) 352, 356, 392, 396, 1239, 1472, 2595, 2753
BstDSI CCRYGG 1 cut(s) 2156
BstF5I GGATG 6 cut(s) 1492, 1780, 2002, 2210, 2924, 3190
BstH2I RGCGCY 1 cut(s) 1438
BstHHI GCGC 1 cut(s) 1437
BstMAI GTCTC 2 cut(s) 1760, 2266
BstMWI GCNNNNNNNGC 9 cut(s) 351, 400, 594, 1031, 2455, 2590, 2842, 2980, 3196
BstNI CCWGG 2 cut(s) 1163, 1592
BstNSI RCATGY 3 cut(s) 1280, 2726, 3035
BstPAI GACNNNNGTC 1 cut(s) 651
BstSCI CCNGG 4 cut(s) 666, 1161, 1262, 1590
BstSFI CTRYAG 5 cut(s) 1023, 1704, 2009, 2199, 2586
BstV2I GAAGAC 3 cut(s) 523, 1137, 2820
BstX2I RGATCY 3 cut(s) 546, 1158, 3382
BstXI CCANNNNNNTGG 4 cut(s) 58, 1370, 1598, 2177
BstYI RGATCY 3 cut(s) 546, 1158, 3382
BstZ17I GTATAC 1 cut(s) 583
BsuI GTATCC 3 cut(s) 291, 1340, 3219
BsuRI GGCC 6 cut(s) 215, 1292, 1362, 1384, 1506, 1561
BtgI CCRYGG 1 cut(s) 2156
BtrI CACGTC 1 cut(s) 1604
BtsCI GGATG 6 cut(s) 1492, 1780, 2002, 2210, 2924, 3190
BtsI GCAGTG 3 cut(s) 1507, 1810, 2733
BtsIMutI CAGTG 6 cut(s) 200, 1507, 1627, 1810, 2733, 3150
BveI ACCTGC 2 cut(s) 1016, 2062
Cac8I GCNNGC 8 cut(s) 352, 356, 392, 396, 1239, 1472, 2595, 2753
CaiI CAGNNNCTG 2 cut(s) 627, 3181
CfoI GCGC 1 cut(s) 1437
Cfr13I GGNCC 7 cut(s) 48, 213, 989, 1360, 1559, 2683, 3094
CseI GACGC 2 cut(s) 1669, 2628
Eam1104I CTCTTC 5 cut(s) 92, 98, 2673, 2877, 3084
EarI CTCTTC 5 cut(s) 92, 98, 2673, 2877, 3084
Ecl136II GAGCTC 2 cut(s) 3190, 3199
Eco130I CCWWGG 4 cut(s) 328, 1106, 2156, 2840
Eco24I GRGCYC 3 cut(s) 158, 3192, 3201
Eco32I GATATC 1 cut(s) 1324
Eco47I GGWCC 4 cut(s) 48, 989, 2683, 3094
Eco53kI GAGCTC 2 cut(s) 3190, 3199
Eco57I CTGAAG 3 cut(s) 1692, 2153, 2419
Eco88I CYCGRG 1 cut(s) 1071
EcoICRI GAGCTC 2 cut(s) 3190, 3199
EcoRI GAATTC 1 cut(s) 2039
EcoRII CCWGG 2 cut(s) 1161, 1590
EcoRV GATATC 1 cut(s) 1324
EcoT14I CCWWGG 4 cut(s) 328, 1106, 2156, 2840
EcoT22I ATGCAT 2 cut(s) 269, 908
EcoT38I GRGCYC 3 cut(s) 158, 3192, 3201
ErhI CCWWGG 4 cut(s) 328, 1106, 2156, 2840
FalI AAGNNNNNCTT 6 cut(s) 441, 473, 749, 781, 2856, 2888
FaqI GGGAC 4 cut(s) 1396, 1573, 1991, 2712
FauNDI CATATG 1 cut(s) 643
FbaI TGATCA 2 cut(s) 1786, 2092
FblI GTMKAC 1 cut(s) 582
FokI GGATG 6 cut(s) 1479, 1787, 2009, 2217, 2931, 3197
FriOI GRGCYC 3 cut(s) 158, 3192, 3201
FspBI CTAG 6 cut(s) 513, 737, 893, 1319, 2514, 2901
GlaI GCGC 1 cut(s) 1436
GsaI CCCAGC 1 cut(s) 1219
GsuI CTGGAG 2 cut(s) 141, 3357
HaeII RGCGCY 1 cut(s) 1438
HaeIII GGCC 6 cut(s) 215, 1292, 1362, 1384, 1506, 1561
HapII CCGG 2 cut(s) 667, 1263
HgaI GACGC 2 cut(s) 1669, 2628
HhaI GCGC 1 cut(s) 1437
Hin1I GRCGYC 1 cut(s) 2817
Hin6I GCGC 1 cut(s) 1435
HinP1I GCGC 1 cut(s) 1435
HincII GTYRAC 2 cut(s) 226, 813
HindII GTYRAC 2 cut(s) 226, 813
HindIII AAGCTT 2 cut(s) 392, 1622
HpaI GTTAAC 1 cut(s) 226
HpaII CCGG 2 cut(s) 667, 1263
Hpy166II GTNNAC 7 cut(s) 226, 583, 813, 1835, 2210, 2312, 3013
Hpy8I GTNNAC 7 cut(s) 226, 583, 813, 1835, 2210, 2312, 3013
Hpy99I CGWCG 1 cut(s) 1992
HpyCH4III ACNGT 8 cut(s) 1103, 1631, 1771, 2057, 2712, 2860, 3010, 3154
HpyCH4IV ACGT 4 cut(s) 405, 1603, 2290, 2817
HpyF10VI GCNNNNNNNGC 9 cut(s) 351, 400, 594, 1031, 2455, 2590, 2842, 2980, 3196
HpySE526I ACGT 4 cut(s) 405, 1603, 2290, 2817
Hsp92I GRCGYC 1 cut(s) 2817
HspAI GCGC 1 cut(s) 1435
Ksp22I TGATCA 2 cut(s) 1786, 2092
KspAI GTTAAC 1 cut(s) 226
LmnI GCTCC 7 cut(s) 427, 538, 1594, 2560, 2971, 3196, 3204
LweI GCATC 6 cut(s) 301, 893, 1076, 2173, 2906, 3313
MaeI CTAG 6 cut(s) 513, 737, 893, 1319, 2514, 2901
MaeII ACGT 4 cut(s) 405, 1603, 2290, 2817
MaeIII GTNAC 3 cut(s) 1615, 1675, 2074
MfeI CAATTG 2 cut(s) 303, 340
MflI RGATCY 3 cut(s) 546, 1158, 3382
MhlI GDGCHC 5 cut(s) 158, 432, 2121, 3192, 3201
MlyI GAGTC 2 cut(s) 1031, 1805
MmeI TCCRAC 4 cut(s) 537, 1147, 1892, 3169
Mph1103I ATGCAT 2 cut(s) 269, 908
MroXI GAANNNNTTC 2 cut(s) 1927, 3256
MslI CAYNNNNRTG 1 cut(s) 820
MspA1I CMGCKG 1 cut(s) 986
MspCI CTTAAG 1 cut(s) 2693
MspI CCGG 2 cut(s) 667, 1263
MspR9I CCNGG 4 cut(s) 668, 1163, 1264, 1592
MunI CAATTG 2 cut(s) 303, 340
Mva1269I GAATGC 2 cut(s) 267, 1960
MvaI CCWGG 2 cut(s) 1163, 1592
MwoI GCNNNNNNNGC 9 cut(s) 351, 400, 594, 1031, 2455, 2590, 2842, 2980, 3196
NciI CCSGG 2 cut(s) 668, 1264
NcoI CCATGG 1 cut(s) 2156
NdeI CATATG 1 cut(s) 643
NlaIV GGNNCC 3 cut(s) 1160, 1221, 1560
NmuCI GTSAC 2 cut(s) 1675, 2074
NsiI ATGCAT 2 cut(s) 269, 908
NspI RCATGY 3 cut(s) 1280, 2726, 3035
PaeR7I CTCGAG 1 cut(s) 1071
PaqCI CACCTGC 1 cut(s) 1016
PciI ACATGT 2 cut(s) 1276, 2722
PctI GAATGC 2 cut(s) 267, 1960
PdmI GAANNNNTTC 2 cut(s) 1927, 3256
PfeI GAWTC 9 cut(s) 80, 673, 1079, 1610, 1634, 1858, 1937, 2534, 3252
PleI GAGTC 2 cut(s) 1031, 1804
PpsI GAGTC 2 cut(s) 1031, 1804
PscI ACATGT 2 cut(s) 1276, 2722
PshAI GACNNNNGTC 1 cut(s) 651
Psp124BI GAGCTC 2 cut(s) 3192, 3201
Psp6I CCWGG 2 cut(s) 1161, 1590
PspFI CCCAGC 1 cut(s) 1215
PspGI CCWGG 2 cut(s) 1161, 1590
PspN4I GGNNCC 3 cut(s) 1160, 1221, 1560
PspPI GGNCC 7 cut(s) 48, 213, 989, 1360, 1559, 2683, 3094
PstI CTGCAG 3 cut(s) 1027, 2013, 2203
PstNI CAGNNNCTG 2 cut(s) 627, 3181
PsuI RGATCY 3 cut(s) 546, 1158, 3382
PvuII CAGCTG 1 cut(s) 986
RseI CAYNNNNRTG 1 cut(s) 820
SacI GAGCTC 2 cut(s) 3192, 3201
Sau96I GGNCC 7 cut(s) 48, 213, 989, 1360, 1559, 2683, 3094
SbfI CCTGCAGG 1 cut(s) 2203
SchI GAGTC 2 cut(s) 1031, 1805
ScrFI CCNGG 4 cut(s) 668, 1163, 1264, 1592
SdaI CCTGCAGG 1 cut(s) 2203
SduI GDGCHC 5 cut(s) 158, 432, 2121, 3192, 3201
SfaNI GCATC 6 cut(s) 301, 893, 1076, 2173, 2906, 3313
SfcI CTRYAG 5 cut(s) 1023, 1704, 2009, 2199, 2586
Sfr274I CTCGAG 1 cut(s) 1071
SinI GGWCC 4 cut(s) 48, 989, 2683, 3094
SlaI CTCGAG 1 cut(s) 1071
SmiMI CAYNNNNRTG 1 cut(s) 820
SmlI CTYRAG 9 cut(s) 91, 794, 1039, 1071, 1848, 2555, 2693, 2828, 2966
SmoI CTYRAG 9 cut(s) 91, 794, 1039, 1071, 1848, 2555, 2693, 2828, 2966
Sse8387I CCTGCAGG 1 cut(s) 2203
SsiI CCGC 3 cut(s) 244, 3170, 3359
SspMI CTAG 6 cut(s) 513, 737, 893, 1319, 2514, 2901
SstI GAGCTC 2 cut(s) 3192, 3201
StyD4I CCNGG 4 cut(s) 666, 1161, 1262, 1590
StyI CCWWGG 4 cut(s) 328, 1106, 2156, 2840
TaaI ACNGT 8 cut(s) 1103, 1631, 1771, 2057, 2712, 2860, 3010, 3154
TaiI ACGT 4 cut(s) 408, 1606, 2293, 2820
TaqI TCGA 5 cut(s) 676, 841, 1072, 1987, 2537
TaqII GACCGA 1 cut(s) 1755
TatI WGTACW 8 cut(s) 146, 1098, 1700, 1844, 2377, 2725, 2860, 2998
TauI GCSGC 1 cut(s) 3361
TfiI GAWTC 9 cut(s) 80, 673, 1079, 1610, 1634, 1858, 1937, 2534, 3252
TscAI CASTG 6 cut(s) 200, 1507, 1634, 1810, 2740, 3157
TseFI GTSAC 2 cut(s) 1675, 2074
Tsp45I GTSAC 2 cut(s) 1675, 2074
TspGWI ACGGA 2 cut(s) 2297, 2641
TspRI CASTG 6 cut(s) 200, 1507, 1634, 1810, 2740, 3157
Vha464I CTTAAG 1 cut(s) 2693
VpaK11BI GGWCC 4 cut(s) 48, 989, 2683, 3094
XapI RAATTY 8 cut(s) 1283, 1923, 2039, 2255, 2550, 2688, 2823, 2961
XbaI TCTAGA 3 cut(s) 736, 892, 2900
XceI RCATGY 3 cut(s) 1280, 2726, 3035
XcmI CCANNNNNNNNNTGG 4 cut(s) 1230, 1300, 1343, 1499
XhoI CTCGAG 1 cut(s) 1071
XmiI GTMKAC 1 cut(s) 582
XmnI GAANNNNTTC 2 cut(s) 1927, 3256
XspI CTAG 6 cut(s) 513, 737, 893, 1319, 2514, 2901
ZraI GACGTC 1 cut(s) 2818
Zsp2I ATGCAT 2 cut(s) 269, 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.