Rh7DG358500

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000280
Physical Location & Seq
Forward (+)
47758144 .. 47762751
4608 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG358500.1

Sequence Viewer

Length: 4608 bp
ATGGAATTCCAGGCCACGGCGGCGAGATTAAGGCCCCGAGGCACAAAGCGGCAGAACGGCGGTGGTGTGGTGGAGAAGCAGGCCGAGGTTATTTTGGGAATTCAGAACGACGACGGAACAATTGGGGAAGACGTGGAGCCAGCAGCGCCGAAGCTCGTTCGCTGGGGGAGGAAGCGGCGGAGGACAGGATGCGAGGGTATTTTGGGAAATCAAAATGAAGGAGAGGCAAGGGCGGCAAATCGGTGCACAAGGGCTCCCTTGGACGTGGGGAAATGTTGCCATCAATGTAAGCGAAATTGTAAGCGAAGAGGGGTGCGGTGCACAAGGTGTCCCGAAAGAGGTAAGCGAAGTAGGTTTTGTCTCCCTTGCATACGGAGATGGTATCCCAACACCTCACAGGAAGCCATAGCTGAGGCTTGTCCTTTGTGCCGCGGCAACTGCAACTGCACAGCCTGCCTTCGCTTGGATGCGCGTTTCAGGCGTTTCGAGAATCGAGACCCGAAAATCGATGAGGAGACCAAGCTGGAGCACTCTAAGTATTTGGTTCATCAGCTGCTCCCGTATTTGAAGATGATTAGGGATGAGCAGTTGAGTGAGATGGGGGTGGAAGCCAAGAGGCAGGGGTTGCGTGACCTTTCGGAGCTCAAGGTGGACAAGGCTAATTGCGGTGACGGAGTGCGTATGTACTGCAACAGCTGCAGCACTTCGATTTTTGATTTTCATAGGACTTGTCCCGGATGTGAGTATGATCTGTGCCTCGGTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAACAAGAGAGCTCAAGCGTCCATATTTGAGTGGAAATGGAGAGCAAGTGAAGATGGAAGCATTCTCTGCCCCCCAACACACATGAAAGGTTGTGGTAAGCATAATTTAGAATTGAGATGCCTTTTTCCTCAGAATCATGTTACGGAATTGGTAGAGAAAGCAGGAGAAATTGATGCAAGTTACAATTTTTTGCCTGATCGTAGTGAAACATGTGCATATAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTCAAGTAGTGGCAGCAGCAGCTCTAAGTTAAGGAAAGCAGCTTCTAGAGTTGCTTCTGATAGTGACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAAGATAAGGATTTTGGGCATTTTCAGTGGCATTGGAAGAGGGGGGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCTGTCATGTGGCGGGCATTCAGACAGATAAAGAAAATCAAGCATGATAGTGATTTGACAGTCCCCGCCATTGATTGCTGGAATTGGTCTTTTATAGAAATCAACCTCCTCCATTTCTTTAATGGCTATTCCAACGGACGGTCTTATGAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATCATCTACTGAGTTTCACAATCATCTTCCCCGGCATGGTGAGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATACTCACCCCAACAAGGGAACTCTGAACCTTGCTATCTACTTGCCACCTACCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACAGGGAGATTCCGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACAACTGAACAACTTGCAACTATAGAGAAGTTGAAGAAAAAGCACAGGGAGCAAAACGAAAGAGAGATATTTGGGGATTGTCGCACAAGTGCGGGAAATGGAAGTGGGCCTCAGCAAGAGTCAGCTATAGAGAAGTTGAAGAGAAAGTATAGGGAGCAAAACGAAAGAGAGATATTTGGGAATTGTCACACAAGTGCTGGAAGTGGAAGTGGGCCTCAGGATGAGTCTGCTGAAGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGATCCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTATGCATGTGCTCTGTAATCCTTTGCAGCATGTTACTCATGCTATACATGATCAGACTGTTTATCTCACTGTAGAGCACAAGAGGAAGCTCAAGGAGGAATTTGGTATCGAGCCGTGGACATTTGTTCAAAGACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTTTCAAAGTTGCACTGGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACAAACCACAGCGCCAGTGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTATTAAGGAGATTTCTGGTGAGAATGTGATGGTAAAGAGGGCAAGGGATGTGAAGGCAAAGAGGGCAAGGGCTCTGAAGGCAGAGAGGGCAAGGGCTCTGAAGGCAAAGAGGGCAAGGGCTCCCTTGGACGTGTCAACAATTTGCCATCAGTGTCAGAGAAATGTTAAGGGAAGAGTGGTGTGGTGCACAAGGTGTGCGAGGAAGAGGTATTGTATTCCTTGCATACAGAGATGGTATCCGAACACATCAGAGGAAGCCATTGCTAAGGCTTGTCCTGTGTGCCGTGGCAACTGCAACTGCACAGCCTGCCTTCGATTGGATGTGGCTGTCAGGCGTTTGCAGAATCTAGACCCGAAAATCGACAAGGGTACCAAGCTGGAGCACTGTAAGTACTTGGTTCATCAGCTGCTTCCCTATTTGAAGAGGATTAGGGATGAGCAGGTGATTGAGATGCTGATCGAGGCTAAGAGGCAGGGATTACGTGATGTTTCGGAGCTCAAGGTGGAGAAGGCTAATTGCGGTGAAGATGAGCGTATGTACTGCAACAACTGCAGCACTTCGATTTTTGATTTTCATAGGAGTTGTCCTGAGTGTGAGTATGATCTGTGCCTCAGTTGCTGCCGGGAGATTCGAGATGGCAGGTGGAAGAAGAGAGGGATGGAGGAGAAAGCTCAAGTGTCCACATTTGAGTGGAGAGCAAGTGAAGATGGAAGCATTCTCTGGACACATATGAAATGCTGTGGTAAGCATAAATTAGAATTGAAATGCAAATTTTCTCAGAATCATGTCATAGAATTGGTAGAGAAAGCAGGAAAAATAGATGAAAGTTACAGTCATTTGCGTGCTAGTAGTGAAACACCTGCACACAAGTGTTCATGTGTTAAACCTGTGGATGATGCCATTAGCTCAAGTAGCAGCTGCTCTATGTTAAGGAAAGCAGCTTCTAGAGATGGTTCTGATGATAACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAAGATAAGGATTTTGGGCATTTTCGGTGGCATTGGATGAGGGGTGAGCCTGTGATCGTTAGCAATACACTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCTGTCATGTGGCGGGCATTTAGAAAGATAAAGAAGATCAAGCATGATAGTGAATTGACAGTCCACGCCATTGATTGCTGGGATTGGTGTTTTATAGAAATCAACATCCTCCATTTCTTTAGTGGATATTCCAACGGACGGTGTTATAAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATCATCTGCTGAGTTTCACAATCGGCTTCCCCGGCATGTTAAGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATACTCACCCCAACAAGGGAACTCTGAACCTTGCTATCTACTTGCCACCTACCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACGGGGAGATTCTGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTTAATATTCTGACGCATACAGCTGAAGTGCCCCTTACAACTGAACAACTGGCAACTATAGAGAAGTTGAAGAAAAAGCACAGGGAGCAAGACAAAAGAGATATATTTGGGAATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCGCAGCAAGAATCCGCTGAAGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGATTCAGGATGTTCCGCTGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTATGCATGTGCTCTGTAATCCTTTGCAGCGTGTTACTCATGCTATACATGATCAGACTGTTTATCTCACTGTGGAGCACAAGAGGAAGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGCGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTGGAGTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACAAACCACAGAGCCAGTGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTGTGAAGGAGATTTCTGCTGAGAATGTGATGACTGAGGTTGTGAAGCCTAGGGGAGGCGCAAGGGAATTAAGGCAAGGTGTAAGGTCCAAGTCTTACAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1535

Amino Acids

175.04

Weight (kDa)

8.64

Isoelectric Point (pI)

49.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 89 - 155 8e-06 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 654 - 750 9e-13 JmjC domain, hydroxylase
zf-4CXXC_R1 PF10497 838 - 899 4.4e-07 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 1359 - 1458 5.2e-13 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3624
AarI CACCTGC 3 cut(s) 2809, 3009, 3247
Acc36I ACCTGC 5 cut(s) 2183, 2809, 3009, 3247, 4307
Acc65I GGTACC 1 cut(s) 2747
AccB1I GGYRCC 3 cut(s) 1958, 2747, 4082
AccB7I CCANNNNNTGG 2 cut(s) 1136, 3365
AccI GTMKAC 2 cut(s) 1511, 3740
AccII CGCG 2 cut(s) 432, 472
AccIII TCCGGA 2 cut(s) 1977, 4101
AclWI GGATC 4 cut(s) 1234, 1975, 1988, 3463
AcsI RAATTY 9 cut(s) 5, 99, 1481, 2030, 2132, 3149, 3710, 4154, 4256
AcuI CTGAAG 6 cut(s) 1716, 1959, 2474, 2498, 3945, 4083
AdeI CACNNNGTG 2 cut(s) 327, 2571
AfaI GTAC 4 cut(s) 686, 2749, 2771, 2918
AflIII ACRYGT 2 cut(s) 1001, 2508
AjiI CACGTC 5 cut(s) 133, 265, 2023, 2509, 4147
AjnI CCWGG 2 cut(s) 9, 1983
AleI CACNNNNGTG 3 cut(s) 1899, 3247, 4023
Alw26I GTCTC 4 cut(s) 365, 489, 509, 1391
Alw44I GTGCAC 3 cut(s) 244, 319, 2563
AlwI GGATC 4 cut(s) 1234, 1975, 1988, 3463
AlwNI CAGNNNCTG 1 cut(s) 2997
Ama87I CYCGRG 1 cut(s) 36
Aor13HI TCCGGA 2 cut(s) 1977, 4101
AoxI GGCC 9 cut(s) 12, 32, 81, 1406, 1701, 1814, 1919, 3635, 4043
ApaLI GTGCAC 3 cut(s) 244, 319, 2563
ApoI RAATTY 9 cut(s) 5, 99, 1481, 2030, 2132, 3149, 3710, 4154, 4256
Asp700I GAANNNNTTC 2 cut(s) 2295, 4419
Asp718I GGTACC 1 cut(s) 2747
AspA2I CCTAGG 1 cut(s) 4550
AspLEI GCGC 4 cut(s) 148, 472, 2324, 4562
AspS9I GGNCC 8 cut(s) 33, 1583, 1702, 1814, 1919, 3812, 4043, 4587
AsuC2I CCSGG 5 cut(s) 735, 770, 1468, 3002, 3697
AvaI CYCGRG 1 cut(s) 36
AvaII GGWCC 3 cut(s) 1583, 3812, 4587
AvrII CCTAGG 1 cut(s) 4550
AxyI CCTNAGG 1 cut(s) 1923
BaeGI GKGCMC 4 cut(s) 248, 323, 2567, 3933
BaeI ACNNNNGTAYC 2 cut(s) 2731, 2764
BamHI GGATCC 1 cut(s) 1980
BanI GGYRCC 3 cut(s) 1958, 2747, 4082
BanII GRGCYC 7 cut(s) 256, 645, 805, 2452, 2476, 2500, 2877
BarI GAAGNNNNNNTAC 4 cut(s) 1632, 1664, 3861, 3893
BbsI GAAGAC 1 cut(s) 135
BbvCI CCTCAGC 2 cut(s) 411, 1818
BceAI ACGGC 6 cut(s) 33, 73, 1028, 2131, 2646, 4255
BciT130I CCWGG 2 cut(s) 11, 1985
BciVI GTATCC 2 cut(s) 393, 2625
BclI TGATCA 2 cut(s) 2083, 4207
BcnI CCSGG 5 cut(s) 735, 770, 1468, 3002, 3697
BcoDI GTCTC 4 cut(s) 365, 489, 509, 1391
BfaI CTAG 5 cut(s) 1095, 2726, 3225, 3324, 4551
BfmI CTRYAG 8 cut(s) 697, 1728, 1833, 2103, 2190, 2929, 3957, 4314
BfoI RGCGCY 2 cut(s) 149, 2325
BfuAI ACCTGC 5 cut(s) 2183, 2809, 3009, 3247, 4307
BfuI GTATCC 2 cut(s) 393, 2625
BglI GCCNNNNNGGC 1 cut(s) 20
BlnI CCTAGG 1 cut(s) 4550
BmcAI AGTACT 1 cut(s) 2771
Bme1390I CCNGG 7 cut(s) 11, 735, 770, 1468, 1985, 3002, 3697
Bme18I GGWCC 3 cut(s) 1583, 3812, 4587
BmeT110I CYCGRG 1 cut(s) 36
BmgBI CACGTC 5 cut(s) 133, 265, 2023, 2509, 4147
BmgT120I GGNCC 8 cut(s) 33, 1583, 1702, 1814, 1919, 3812, 4043, 4587
BmrFI CCNGG 7 cut(s) 11, 735, 770, 1468, 1985, 3002, 3697
BoxI GACNNNNGTC 3 cut(s) 1581, 3810, 4280
BpiI GAAGAC 1 cut(s) 135
BpmI CTGGAG 7 cut(s) 545, 2021, 2360, 2777, 4145, 4388, 4484
Bpu10I CCTNAGC 3 cut(s) 411, 1818, 2643
BpuMI CCSGG 5 cut(s) 735, 770, 1468, 3002, 3697
Bsa29I ATCGAT 1 cut(s) 507
BsaAI YACGTR 1 cut(s) 2861
BsaBI GATNNNNATC 1 cut(s) 2834
BsaI GGTCTC 2 cut(s) 489, 509
BsaWI WCCGGW 2 cut(s) 1977, 4101
Bse1I ACTGG 5 cut(s) 2247, 2325, 3954, 4371, 4449
Bse21I CCTNAGG 1 cut(s) 1923
Bse3DI GCAATG 2 cut(s) 1216, 2637
Bse8I GATNNNNATC 1 cut(s) 2834
BseAI TCCGGA 2 cut(s) 1977, 4101
BseBI CCWGG 2 cut(s) 11, 1985
BseCI ATCGAT 1 cut(s) 507
BseJI GATNNNNATC 1 cut(s) 2834
BseMI GCAATG 2 cut(s) 1216, 2637
BseNI ACTGG 5 cut(s) 2247, 2325, 3954, 4371, 4449
BseRI GAGGAG 3 cut(s) 527, 1346, 3056
BseSI GKGCMC 4 cut(s) 248, 323, 2567, 3933
BseYI CCCAGC 4 cut(s) 162, 1239, 3468, 3555
BsgI GTGCAG 3 cut(s) 430, 2662, 3225
Bsh1236I CGCG 2 cut(s) 432, 472
BshFI GGCC 9 cut(s) 14, 34, 83, 1408, 1703, 1816, 1921, 3637, 4045
BshNI GGYRCC 3 cut(s) 1958, 2747, 4082
BshVI ATCGAT 1 cut(s) 507
BsiHKCI CYCGRG 1 cut(s) 36
BsiSI CCGG 9 cut(s) 735, 769, 1468, 1574, 1978, 3001, 3697, 3803, 4102
BslFI GGGAC 5 cut(s) 315, 717, 1295, 1982, 4106
BsmAI GTCTC 4 cut(s) 365, 489, 509, 1391
BsmFI GGGAC 5 cut(s) 315, 717, 1295, 1982, 4106
BsmI GAATGC 3 cut(s) 852, 1265, 3093
BsnI GGCC 9 cut(s) 14, 34, 83, 1408, 1703, 1816, 1921, 3637, 4045
Bso31I GGTCTC 2 cut(s) 489, 509
BsoBI CYCGRG 1 cut(s) 36
Bsp13I TCCGGA 2 cut(s) 1977, 4101
BspANI GGCC 9 cut(s) 14, 34, 83, 1408, 1703, 1816, 1921, 3637, 4045
BspDI ATCGAT 1 cut(s) 507
BspEI TCCGGA 2 cut(s) 1977, 4101
BspFNI CGCG 2 cut(s) 432, 472
BspMAI CTGCAG 4 cut(s) 701, 2194, 2933, 4318
BspMI ACCTGC 5 cut(s) 2183, 2809, 3009, 3247, 4307
BspPI GGATC 4 cut(s) 1234, 1975, 1988, 3463
BspT107I GGYRCC 3 cut(s) 1958, 2747, 4082
BspTNI GGTCTC 2 cut(s) 489, 509
BsrDI GCAATG 2 cut(s) 1216, 2637
BsrI ACTGG 5 cut(s) 2247, 2325, 3954, 4371, 4449
BssNAI GTATAC 2 cut(s) 1512, 3741
BssT1I CCWWGG 5 cut(s) 258, 1130, 2502, 3359, 4550
Bst1107I GTATAC 2 cut(s) 1512, 3741
Bst2UI CCWGG 2 cut(s) 11, 1985
Bst6I CTCTTC 9 cut(s) 301, 1178, 1841, 2286, 2545, 2576, 2795, 3023, 4410
BstAPI GCANNNNNTGC 6 cut(s) 453, 625, 696, 858, 2685, 2928
BstBAI YACGTR 1 cut(s) 2861
BstC8I GCNNGC 9 cut(s) 81, 141, 454, 1263, 2364, 2686, 3222, 3492, 4488
BstDSI CCRYGG 5 cut(s) 15, 430, 2147, 2662, 4271
BstENI CCTNNNNNAGG 1 cut(s) 4554
BstFNI CGCG 2 cut(s) 432, 472
BstH2I RGCGCY 2 cut(s) 149, 2325
BstHHI GCGC 4 cut(s) 148, 472, 2324, 4562
BstMAI GTCTC 4 cut(s) 365, 489, 509, 1391
BstNI CCWGG 2 cut(s) 11, 1985
BstNSI RCATGY 7 cut(s) 1005, 2042, 2066, 2366, 3704, 4166, 4490
BstPAI GACNNNNGTC 3 cut(s) 1581, 3810, 4280
BstSCI CCNGG 7 cut(s) 9, 733, 768, 1466, 1983, 3000, 3695
BstSFI CTRYAG 8 cut(s) 697, 1728, 1833, 2103, 2190, 2929, 3957, 4314
BstSLI GKGCMC 4 cut(s) 248, 323, 2567, 3933
BstUI CGCG 2 cut(s) 432, 472
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 1 cut(s) 1980
BstXI CCANNNNNNTGG 1 cut(s) 4292
BstYI RGATCY 1 cut(s) 1980
BstZ17I GTATAC 2 cut(s) 1512, 3741
Bsu15I ATCGAT 1 cut(s) 507
Bsu36I CCTNAGG 1 cut(s) 1923
BsuI GTATCC 2 cut(s) 393, 2625
BsuRI GGCC 9 cut(s) 14, 34, 83, 1408, 1703, 1816, 1921, 3637, 4045
BsuTUI ATCGAT 1 cut(s) 507
BtgI CCRYGG 5 cut(s) 15, 430, 2147, 2662, 4271
BtgZI GCGATG 1 cut(s) 4311
BtrI CACGTC 5 cut(s) 133, 265, 2023, 2509, 4147
BtsI GCAGTG 2 cut(s) 1651, 3880
BveI ACCTGC 5 cut(s) 2183, 2809, 3009, 3247, 4307
Cac8I GCNNGC 9 cut(s) 81, 141, 454, 1263, 2364, 2686, 3222, 3492, 4488
CaiI CAGNNNCTG 1 cut(s) 2997
CfoI GCGC 4 cut(s) 148, 472, 2324, 4562
Cfr13I GGNCC 8 cut(s) 33, 1583, 1702, 1814, 1919, 3812, 4043, 4587
Cfr42I CCGCGG 1 cut(s) 433
ClaI ATCGAT 1 cut(s) 507
CseI GACGC 2 cut(s) 798, 3922
Csp6I GTAC 4 cut(s) 685, 2748, 2770, 2917
CviQI GTAC 4 cut(s) 685, 2748, 2770, 2917
DraIII CACNNNGTG 2 cut(s) 327, 2571
Eam1104I CTCTTC 9 cut(s) 301, 1178, 1841, 2286, 2545, 2576, 2795, 3023, 4410
EarI CTCTTC 9 cut(s) 301, 1178, 1841, 2286, 2545, 2576, 2795, 3023, 4410
EciI GGCGGA 1 cut(s) 193
Ecl136II GAGCTC 3 cut(s) 643, 803, 2875
Eco130I CCWWGG 5 cut(s) 258, 1130, 2502, 3359, 4550
Eco24I GRGCYC 7 cut(s) 256, 645, 805, 2452, 2476, 2500, 2877
Eco31I GGTCTC 2 cut(s) 489, 509
Eco47I GGWCC 3 cut(s) 1583, 3812, 4587
Eco53kI GAGCTC 3 cut(s) 643, 803, 2875
Eco57I CTGAAG 6 cut(s) 1716, 1959, 2474, 2498, 3945, 4083
Eco81I CCTNAGG 1 cut(s) 1923
Eco88I CYCGRG 1 cut(s) 36
EcoICRI GAGCTC 3 cut(s) 643, 803, 2875
EcoNI CCTNNNNNAGG 1 cut(s) 4554
EcoO109I RGGNCCY 1 cut(s) 33
EcoRI GAATTC 3 cut(s) 5, 99, 4256
EcoRII CCWGG 2 cut(s) 9, 1983
EcoT14I CCWWGG 5 cut(s) 258, 1130, 2502, 3359, 4550
EcoT22I ATGCAT 2 cut(s) 2040, 4164
EcoT38I GRGCYC 7 cut(s) 256, 645, 805, 2452, 2476, 2500, 2877
ErhI CCWWGG 5 cut(s) 258, 1130, 2502, 3359, 4550
FalI AAGNNNNNCTT 4 cut(s) 1689, 1721, 3918, 3950
FaqI GGGAC 5 cut(s) 315, 717, 1295, 1982, 4106
FauI CCCGC 4 cut(s) 1254, 1321, 1792, 3483
FauNDI CATATG 1 cut(s) 3108
FbaI TGATCA 2 cut(s) 2083, 4207
FblI GTMKAC 2 cut(s) 1511, 3740
FriOI GRGCYC 7 cut(s) 256, 645, 805, 2452, 2476, 2500, 2877
FspBI CTAG 5 cut(s) 1095, 2726, 3225, 3324, 4551
GlaI GCGC 4 cut(s) 147, 471, 2323, 4561
GsaI CCCAGC 4 cut(s) 166, 1243, 3472, 3559
GsuI CTGGAG 7 cut(s) 545, 2021, 2360, 2777, 4145, 4388, 4484
HaeII RGCGCY 2 cut(s) 149, 2325
HaeIII GGCC 9 cut(s) 14, 34, 83, 1408, 1703, 1816, 1921, 3637, 4045
HapII CCGG 9 cut(s) 735, 769, 1468, 1574, 1978, 3001, 3697, 3803, 4102
HgaI GACGC 2 cut(s) 798, 3922
HhaI GCGC 4 cut(s) 148, 472, 2324, 4562
Hin6I GCGC 4 cut(s) 146, 470, 2322, 4560
HinP1I GCGC 4 cut(s) 146, 470, 2322, 4560
HincII GTYRAC 1 cut(s) 2514
HindII GTYRAC 1 cut(s) 2514
HindIII AAGCTT 2 cut(s) 1646, 3875
HpaII CCGG 9 cut(s) 735, 769, 1468, 1574, 1978, 3001, 3697, 3803, 4102
Hpy99I CGWCG 2 cut(s) 113, 116
HpyAV CCTTC 8 cut(s) 212, 467, 2425, 2449, 2473, 2699, 2881, 4501
HpyCH4IV ACGT 6 cut(s) 132, 264, 2022, 2508, 2860, 4146
HpySE526I ACGT 6 cut(s) 132, 264, 2022, 2508, 2860, 4146
HspAI GCGC 4 cut(s) 146, 470, 2322, 4560
Kpn2I TCCGGA 2 cut(s) 1977, 4101
KpnI GGTACC 1 cut(s) 2751
Ksp22I TGATCA 2 cut(s) 2083, 4207
KspI CCGCGG 1 cut(s) 433
MaeI CTAG 5 cut(s) 1095, 2726, 3225, 3324, 4551
MaeII ACGT 6 cut(s) 132, 264, 2022, 2508, 2860, 4146
MfeI CAATTG 1 cut(s) 120
MflI RGATCY 1 cut(s) 1980
MlyI GAGTC 2 cut(s) 1835, 1940
MmeI TCCRAC 6 cut(s) 1117, 1404, 2333, 3346, 3633, 4457
Mph1103I ATGCAT 2 cut(s) 2040, 4164
MroI TCCGGA 2 cut(s) 1977, 4101
MroXI GAANNNNTTC 2 cut(s) 2295, 4419
MspI CCGG 9 cut(s) 735, 769, 1468, 1574, 1978, 3001, 3697, 3803, 4102
MspR9I CCNGG 7 cut(s) 11, 735, 770, 1468, 1985, 3002, 3697
MunI CAATTG 1 cut(s) 120
Mva1269I GAATGC 3 cut(s) 852, 1265, 3093
MvaI CCWGG 2 cut(s) 11, 1985
MvnI CGCG 2 cut(s) 432, 472
NciI CCSGG 5 cut(s) 735, 770, 1468, 3002, 3697
NdeI CATATG 1 cut(s) 3108
NmeAIII GCCGAG 1 cut(s) 109
NmuCI GTSAC 5 cut(s) 629, 668, 1112, 1892, 4016
NsiI ATGCAT 2 cut(s) 2040, 4164
NspI RCATGY 7 cut(s) 1005, 2042, 2066, 2366, 3704, 4166, 4490
OliI CACNNNNGTG 3 cut(s) 1899, 3247, 4023
PaeI GCATGC 2 cut(s) 2366, 4490
PaqCI CACCTGC 3 cut(s) 2809, 3009, 3247
PciI ACATGT 1 cut(s) 1001
PctI GAATGC 3 cut(s) 852, 1265, 3093
PdmI GAANNNNTTC 2 cut(s) 2295, 4419
PflMI CCANNNNNTGG 2 cut(s) 1136, 3365
PfoI TCCNGGA 2 cut(s) 733, 1983
PleI GAGTC 2 cut(s) 1834, 1939
PpsI GAGTC 2 cut(s) 1834, 1939
Ppu21I YACGTR 1 cut(s) 2861
PscI ACATGT 1 cut(s) 1001
PshAI GACNNNNGTC 3 cut(s) 1581, 3810, 4280
PsiI TTATAA 1 cut(s) 3624
Psp124BI GAGCTC 3 cut(s) 645, 805, 2877
Psp6I CCWGG 2 cut(s) 9, 1983
PspFI CCCAGC 4 cut(s) 162, 1239, 3468, 3555
PspGI CCWGG 2 cut(s) 9, 1983
PspPI GGNCC 8 cut(s) 33, 1583, 1702, 1814, 1919, 3812, 4043, 4587
PstI CTGCAG 4 cut(s) 701, 2194, 2933, 4318
PstNI CAGNNNCTG 1 cut(s) 2997
PsuI RGATCY 1 cut(s) 1980
PvuII CAGCTG 7 cut(s) 553, 696, 1694, 1999, 2785, 3297, 3923
RsaI GTAC 4 cut(s) 686, 2749, 2771, 2918
RsaNI GTAC 4 cut(s) 685, 2748, 2770, 2917
SacI GAGCTC 3 cut(s) 645, 805, 2877
SacII CCGCGG 1 cut(s) 433
Sau96I GGNCC 8 cut(s) 33, 1583, 1702, 1814, 1919, 3812, 4043, 4587
SbfI CCTGCAGG 2 cut(s) 2194, 4318
ScaI AGTACT 1 cut(s) 2771
SchI GAGTC 2 cut(s) 1835, 1940
ScrFI CCNGG 7 cut(s) 11, 735, 770, 1468, 1985, 3002, 3697
SdaI CCTGCAGG 2 cut(s) 2194, 4318
SfcI CTRYAG 8 cut(s) 697, 1728, 1833, 2103, 2190, 2929, 3957, 4314
Sfr303I CCGCGG 1 cut(s) 433
SgrBI CCGCGG 1 cut(s) 433
SinI GGWCC 3 cut(s) 1583, 3812, 4587
SphI GCATGC 2 cut(s) 2366, 4490
Sse8387I CCTGCAGG 2 cut(s) 2194, 4318
SspI AATATT 2 cut(s) 1678, 3907
SspMI CTAG 5 cut(s) 1095, 2726, 3225, 3324, 4551
SstI GAGCTC 3 cut(s) 645, 805, 2877
StyD4I CCNGG 7 cut(s) 9, 733, 768, 1466, 1983, 3000, 3695
StyI CCWWGG 5 cut(s) 258, 1130, 2502, 3359, 4550
TaiI ACGT 6 cut(s) 135, 267, 2025, 2511, 2863, 4149
TatI WGTACW 3 cut(s) 684, 2769, 2916
TauI GCSGC 7 cut(s) 23, 52, 178, 236, 432, 435, 4048
TseFI GTSAC 5 cut(s) 629, 668, 1112, 1892, 4016
Tsp45I GTSAC 5 cut(s) 629, 668, 1112, 1892, 4016
TspGWI ACGGA 9 cut(s) 129, 388, 687, 950, 1398, 1627, 2303, 3627, 4427
Van91I CCANNNNNTGG 2 cut(s) 1136, 3365
VneI GTGCAC 3 cut(s) 244, 319, 2563
VpaK11BI GGWCC 3 cut(s) 1583, 3812, 4587
XagI CCTNNNNNAGG 1 cut(s) 4554
XapI RAATTY 9 cut(s) 5, 99, 1481, 2030, 2132, 3149, 3710, 4154, 4256
XbaI TCTAGA 3 cut(s) 1094, 2725, 3323
XceI RCATGY 7 cut(s) 1005, 2042, 2066, 2366, 3704, 4166, 4490
XcmI CCANNNNNNNNNTGG 4 cut(s) 1254, 1367, 3483, 3596
XmaJI CCTAGG 1 cut(s) 4550
XmiI GTMKAC 2 cut(s) 1511, 3740
XmnI GAANNNNTTC 2 cut(s) 2295, 4419
XspI CTAG 5 cut(s) 1095, 2726, 3225, 3324, 4551
ZrmI AGTACT 1 cut(s) 2771
Zsp2I ATGCAT 2 cut(s) 2040, 4164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.