Rmu_sc0004540.1_g000026

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004540.1
OG0000280
Physical Location & Seq
Forward (+)
149028 .. 153734
4707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004540.1_g000026.1.cds

Sequence Viewer

Length: 4707 bp
atgagaatccaggcgacgccgtcgagattgtggccccgaggcacaaagcggccgaacatatggggaattcagaacgacgacggaacaaatggggaagaagatggtggaggatcgtctgtggagccactgccaaagcttgttccctgggggaggaagcggcggaggctaggatgcgagggtttttgggcaaatcaaaatgaaggaaacgcaagggctgtgaaggcgaagaagaagaagaagcagaagcagcagcagcagcagagaagggctcccttggaggtgcagcagcagacaagggataagcaagtaccactacctagctacctgcagagaagggctcccttggaggtgcagcagcagagtagggataaacaagaaccactacctagctacctgcagagaaggactcccttgaaggtacggacaatcaattgccatcaatgtaagcaaaatgatatgggaagagcggtgcggtgcacaaagtgttcgaggaagaggtattgtatcccttgcataaggacttggtatcccaacacctcagaggaagccatagctgaggcttgtcctgcgtgccgcggcaactgcaactgcaatgtctgcctttgttggggtgtgcctgtcaggaatctgaacccgaaaatcgacaaggaggccaagctggatcactctaagtatttggttcatcagctgcttccgtatttgaagatgattagggatgagcaggtgagtgagatggtggtggaagccaataggcagggcttacgtgacctttcggagctcaaggtggacaaggctaaaagcggtggtgacggagtgcgtatgtactgcaacagctgcagcacttcaatttttgattttcataggacttgtcccgggtgtgagtatgatctgtgcctcggctgctgccgggagattcgagatggcaagtggaacaagagagctcaagcgtccacatttgagtggaaatggagagcaagtgaaggcggaaacattttctgcccaccaacacacatgagaggttgtggtaagcataatttagaattgagatgcatttttcctcagaatcatgtcatggagttggtagagaaagcagaagaaatagattcaagttacaattttttgcctgattgtagtgaaacatgtgcatataagtgttcatgtgttaaacctgtggatgatgccgttagctcaaatagtagcagcagctgtaagttaaggaaagcagcttctagagttgcttctgatagtgactacttgttctgtccaagggttggacaaattcaagataaggattttgggcattttcagtggcattggaagaggggagagcctgtgatcgttagcaatgcacttgaaaatggatcaggtttaagctgggaaccatctgtcttgtggcgggcatttagacagatagagaaagtcaagaatgatagtgagttgacattccacgccattgattgttgggattggtgttctatagaaatcaacatcctccatttctttagtggatattccaacggacagtctcataagacaggatggcctcagaatctcaagctcaatgacttcccatcatctgctgaattttacaaacgtcttccccggcattgtaaggaatttttatgttgtctgcctctcaaggagtatactcaccccaacaagggaactttgaaccttgctacctacttgccacctacctcagtaaagccggacatgggtccaaaaacatatattgcctatggttttgcacaggagcttggacagggagattccgtaaccaagcttcactgcaatatgtctgatgcggttaatattctgacacatacagctgaagtggcccttacaactgaacaacttgcaactatagagaagttgaagaaaaagcacagggagcaaaacgaaagagagatatttggggattgtcacacaagtgcgggaaatggaagtgggcctcagcaagagtcagctatagagaagttgaagagaaagtatagggagcaaaacgaaagagagatatttgggaattgtcacacaagtgctggaagtggaagtgggcctcaggatgagtctgctgaagtatgtgaggatggaggtgccttatgggacattttccggatccaggatgttccgcagctggagaaatacatccacaagcacgtcaatgaatttatgcatgtatatggtaatcctttgcagcatgttactcatgctatacatgatcagactgtatatctcactgtggaacacaagaggaagctcaaggaggaatttggtatcgagccgtggacatttttccaaaaacttggagatgctgttttcattcctgcaggttgtcctcatcaggtgaggaacttgaagtcttgtatcaaagttgcacttgactttgtttcccctgagaatgtgggtgagtgctttcgtttgacggaagagtttcgcacacttccgacaaaccacagagcccatgaggacaagctggaggtgaagaaaatgattgtgcatgcagtttgtgaagctatgaaggagatttctggtgagattgtgacaaagagggtaagggctgtgaaggcaaagagggcaaaggacgagtcgatacactgccatcagtgtcatagatatgttatgggaagagtagtgcagtgcacaaggtgtctggaaagaggtatgggaatgaggacgaggtattgtatcccttgtatacagagatggtatccgaacacatcagaggaagccattgctaaggcttgtcctgtatgccacggcaactgcaactgcacagcctgccttcgtttggacgtgcctgtccgttgtttgcagaatctagaccccaaaatcgacaggggtaccaagctggagcactgtaagtatttggttcatcagctgcttccctatttgaagaggattagggatgagcaggtgagtgagatggtggtcgaggctaagaggcggggattacgtgatgttttggagctcaaggtggagaaggctaagtgcggtgaagatgagcgtatgtactgcaacaactgcagcacttcgatttttgattttcataggagttgtcccaagtgtgggtttgatctgtgcctcagttgctgccgggagattcgagatggcaagtggaagaagagagaggtggaggaggaagctcaagtgtccacatttgagtggagatttagtgaagatggaagcattctctgccccccaacacaaaagaaatgttttggtaagcataatttagaattgagatgcattttttctcagaatcatgtcatggaattggtagagaaagcaggaaaaatagatgcgagttacaattttttgcctgaccgtagtgaaacatgtgcatacaagtgttcatgtgttaaacctgtggatgatgccgttagctcaagcagcagcagctgtaagttaaggaaagcagcttctagagtatcttctgacagtaactacttgttctgtccaagggttgggcaaatccaagataaggattttgggcattttcagtggcattggatgaggggcgagcctgtggtcgttagcaatgcatttgaaaatggatcaggtttaagctgggaaccatccgtcatgtggcgggcacttagaaagacagttaataactgcaatgctagtgaattgacattccatgccgttgattgctgggattggtgttttataagaatcagaatccgccatttctttagtggatattccaagggacggtcttataagaccggatggcctcagaatctcaagctcaatgacttcccattatctactgagtttcacaatcgtcttccccggcatggtacggaattattatgttgtctgcctttcaaggagtatagtcaccccaacaagggaactctgaaccttgctacctacttgcctcctacctcagtaaagccggacatgggtccaaaaacatatattgcctatggttttgcacaggagcttggacagggagattctgtaaccaagcttcactgcaatatgtctgatgcggttaatattctgacacatacagctgaagtggcccttacaactgaacaacttgcaactatagagaagttgaagaaaaagcacagggagcaagacaaaagagagatatttgggaattgtcacacaagtgctggaaatggaagtgggcctctgcaagaattagctgtagtatgtgaggatggaggtgccttatgggacattttccagactcaggatgttccgcagctggagaaatacatcctcaagcatgtcaatgaatttatgcatgtgctctgtaatcctttgcagcatgttactcatgctatacatgatcagactgtttatctcaacgtggagcacaagaggaggctcaaggaggaatttggtatcgagccatggacatttgtccaaaagcttggagatgctgtttttattcctgcaggttgtcctcatcaggtgaggaacttgaagtcttgtgtcaaagttgcactggactttgtttcccctgagaatgtgggtgagtgctttcgtttgacagaagagtttcgcacacttccgacaaaccacagagcccatgaggacaggctggaggtgaagaaaatgattgtgcatgctgtttgtgaagctgtgaaggagatttctggtgagaatgtgatgactgaggatgcggagtctaggggaggcgcaaggaaattaaggcaaggtgtaaggcgtatgtcttacaaaatgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1568

Amino Acids

179.27

Weight (kDa)

8.64

Isoelectric Point (pI)

50.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3671, 3723
AarI CACCTGC 2 cut(s) 712, 2902
AasI GACNNNNNNGTC 1 cut(s) 2798
Acc36I ACCTGC 6 cut(s) 333, 402, 712, 2318, 2902, 4406
Acc65I GGTACC 1 cut(s) 2840
AccB1I GGYRCC 3 cut(s) 2093, 2840, 4181
AccB7I CCANNNNNTGG 2 cut(s) 1271, 3464
AccBSI CCGCTC 1 cut(s) 467
AccI GTMKAC 2 cut(s) 1646, 2695
AccII CGCG 1 cut(s) 576
AccIII TCCGGA 1 cut(s) 2112
AclWI GGATC 6 cut(s) 118, 669, 1369, 2110, 2123, 3562
AcoI YGGCCR 1 cut(s) 50
AcsI RAATTY 8 cut(s) 66, 1278, 1583, 1616, 2165, 2267, 4253, 4355
AcuI CTGAAG 3 cut(s) 1851, 2094, 4044
AcyI GRCGYC 1 cut(s) 17
AdeI CACNNNGTG 2 cut(s) 483, 2646
AfaI GTAC 6 cut(s) 309, 420, 824, 2842, 3011, 3805
AflIII ACRYGT 2 cut(s) 1139, 3335
AjiI CACGTC 2 cut(s) 2158, 2794
AjnI CCWGG 3 cut(s) 9, 143, 2118
AleI CACNNNNGTG 3 cut(s) 1929, 2034, 4122
Alw21I GWGCWC 8 cut(s) 479, 780, 943, 2642, 2856, 2970, 4269, 4335
Alw26I GTCTC 1 cut(s) 1530
Alw44I GTGCAC 2 cut(s) 475, 2638
AlwI GGATC 6 cut(s) 118, 669, 1369, 2110, 2123, 3562
AlwNI CAGNNNCTG 3 cut(s) 1206, 3090, 3399
Ama87I CYCGRG 2 cut(s) 36, 872
Aor13HI TCCGGA 1 cut(s) 2112
ApaLI GTGCAC 2 cut(s) 475, 2638
ApoI RAATTY 8 cut(s) 66, 1278, 1583, 1616, 2165, 2267, 4253, 4355
ArsI GACNNNNNNTTYG 2 cut(s) 2570, 2602
Asp700I GAANNNNTTC 2 cut(s) 2430, 4518
Asp718I GGTACC 1 cut(s) 2840
AspLEI GCGC 1 cut(s) 4661
AspS9I GGNCC 8 cut(s) 33, 1718, 1837, 1949, 2054, 3911, 4030, 4142
AsuC2I CCSGG 6 cut(s) 873, 874, 908, 1603, 3095, 3796
AvaI CYCGRG 2 cut(s) 36, 872
AvaII GGWCC 2 cut(s) 1718, 3911
AxyI CCTNAGG 1 cut(s) 2058
BaeGI GKGCMC 3 cut(s) 479, 2642, 3595
BaeI ACNNNNGTAYC 2 cut(s) 2824, 2857
BamHI GGATCC 1 cut(s) 2115
BanI GGYRCC 3 cut(s) 2093, 2840, 4181
BanII GRGCYC 7 cut(s) 271, 340, 780, 943, 2461, 2970, 4549
BarI GAAGNNNNNNTAC 4 cut(s) 1767, 1799, 3960, 3992
BbsI GAAGAC 2 cut(s) 1589, 3782
Bbv12I GWGCWC 8 cut(s) 479, 780, 943, 2642, 2856, 2970, 4269, 4335
BbvCI CCTCAGC 2 cut(s) 555, 1953
BceAI ACGGC 6 cut(s) 4, 1166, 2266, 2773, 3362, 3629
BciT130I CCWGG 3 cut(s) 11, 145, 2120
BciVI GTATCC 4 cut(s) 515, 537, 2696, 2718
BclI TGATCA 2 cut(s) 2218, 4306
BcnI CCSGG 6 cut(s) 873, 874, 908, 1603, 3095, 3796
BcoDI GTCTC 1 cut(s) 1530
BfaI CTAG 8 cut(s) 167, 318, 387, 1230, 2819, 3423, 3624, 4650
BfuAI ACCTGC 6 cut(s) 333, 402, 712, 2318, 2902, 4406
BfuI GTATCC 4 cut(s) 515, 537, 2696, 2718
Bme1390I CCNGG 9 cut(s) 11, 145, 873, 874, 908, 1603, 2120, 3095, 3796
Bme18I GGWCC 2 cut(s) 1718, 3911
BmeT110I CYCGRG 2 cut(s) 36, 872
BmgBI CACGTC 2 cut(s) 2158, 2794
BmgT120I GGNCC 8 cut(s) 33, 1718, 1837, 1949, 2054, 3911, 4030, 4142
BmrFI CCNGG 9 cut(s) 11, 145, 873, 874, 908, 1603, 2120, 3095, 3796
BoxI GACNNNNGTC 3 cut(s) 1716, 3909, 4379
BpiI GAAGAC 2 cut(s) 1589, 3782
BplI GAGNNNNNCTC 6 cut(s) 253, 285, 322, 354, 391, 423
BpmI CTGGAG 5 cut(s) 2156, 2495, 2870, 4244, 4583
Bpu10I CCTNAGC 3 cut(s) 555, 1953, 2736
BpuMI CCSGG 6 cut(s) 873, 874, 908, 1603, 3095, 3796
BsaAI YACGTR 2 cut(s) 764, 2954
BsaHI GRCGYC 1 cut(s) 17
BsaWI WCCGGW 2 cut(s) 2112, 3728
Bse1I ACTGG 1 cut(s) 4470
Bse21I CCTNAGG 1 cut(s) 2058
Bse3DI GCAATG 5 cut(s) 598, 1351, 2730, 3544, 3625
BseAI TCCGGA 1 cut(s) 2112
BseBI CCWGG 3 cut(s) 11, 145, 2120
BseMI GCAATG 5 cut(s) 598, 1351, 2730, 3544, 3625
BseNI ACTGG 1 cut(s) 4470
BseRI GAGGAG 2 cut(s) 3149, 4354
BseSI GKGCMC 3 cut(s) 479, 2642, 3595
BseX3I CGGCCG 1 cut(s) 50
BseYI CCCAGC 3 cut(s) 1374, 3567, 3654
BsgI GTGCAG 4 cut(s) 302, 371, 2654, 2755
Bsh1236I CGCG 1 cut(s) 576
Bsh1285I CGRYCG 1 cut(s) 53
BshNI GGYRCC 3 cut(s) 2093, 2840, 4181
BsiEI CGRYCG 1 cut(s) 53
BsiHKAI GWGCWC 8 cut(s) 479, 780, 943, 2642, 2856, 2970, 4269, 4335
BsiHKCI CYCGRG 2 cut(s) 36, 872
BsiSI CCGG 9 cut(s) 873, 907, 1603, 1709, 2113, 3094, 3729, 3796, 3902
BslFI GGGAC 5 cut(s) 855, 2117, 3042, 3726, 4205
BsmAI GTCTC 1 cut(s) 1530
BsmFI GGGAC 5 cut(s) 855, 2117, 3042, 3726, 4205
BsmI GAATGC 1 cut(s) 3186
BsoBI CYCGRG 2 cut(s) 36, 872
Bsp13I TCCGGA 1 cut(s) 2112
Bsp19I CCATGG 1 cut(s) 4370
BspEI TCCGGA 1 cut(s) 2112
BspFNI CGCG 1 cut(s) 576
BspMAI CTGCAG 6 cut(s) 330, 399, 839, 2329, 3026, 4417
BspMI ACCTGC 6 cut(s) 333, 402, 712, 2318, 2902, 4406
BspPI GGATC 6 cut(s) 118, 669, 1369, 2110, 2123, 3562
BspQI GCTCTTC 1 cut(s) 457
BspT107I GGYRCC 3 cut(s) 2093, 2840, 4181
BsrBI CCGCTC 1 cut(s) 467
BsrDI GCAATG 5 cut(s) 598, 1351, 2730, 3544, 3625
BsrI ACTGG 1 cut(s) 4470
BssNAI GTATAC 2 cut(s) 1647, 2696
BssNI GRCGYC 1 cut(s) 17
BssT1I CCWWGG 6 cut(s) 273, 342, 1265, 3458, 3708, 4370
Bst1107I GTATAC 2 cut(s) 1647, 2696
Bst2UI CCWGG 3 cut(s) 11, 145, 2120
Bst4CI ACNGT 9 cut(s) 1524, 2227, 2239, 2858, 3326, 3440, 3607, 3717, 4315
Bst6I CTCTTC 9 cut(s) 457, 488, 1313, 1976, 2421, 2620, 2888, 3116, 4509
BstACI GRCGYC 1 cut(s) 17
BstAPI GCANNNNNTGC 5 cut(s) 597, 834, 2778, 3021, 3192
BstBAI YACGTR 2 cut(s) 764, 2954
BstC8I GCNNGC 7 cut(s) 571, 1398, 2499, 2779, 3521, 3591, 4587
BstDSI CCRYGG 4 cut(s) 574, 2282, 2755, 4370
BstENI CCTNNNNNAGG 1 cut(s) 148
BstFNI CGCG 1 cut(s) 576
BstHHI GCGC 1 cut(s) 4661
BstMAI GTCTC 1 cut(s) 1530
BstMCI CGRYCG 1 cut(s) 53
BstNI CCWGG 3 cut(s) 11, 145, 2120
BstNSI RCATGY 9 cut(s) 1143, 2177, 2201, 2501, 3339, 4247, 4265, 4289, 4589
BstPAI GACNNNNGTC 3 cut(s) 1716, 3909, 4379
BstSCI CCNGG 9 cut(s) 9, 143, 871, 872, 906, 1601, 2118, 3093, 3794
BstSLI GKGCMC 3 cut(s) 479, 2642, 3595
BstUI CGCG 1 cut(s) 576
BstV2I GAAGAC 2 cut(s) 1589, 3782
BstX2I RGATCY 1 cut(s) 2115
BstXI CCANNNNNNTGG 2 cut(s) 2303, 4391
BstYI RGATCY 1 cut(s) 2115
BstZ17I GTATAC 2 cut(s) 1647, 2696
BstZI CGGCCG 1 cut(s) 50
Bsu36I CCTNAGG 1 cut(s) 2058
BsuI GTATCC 4 cut(s) 515, 537, 2696, 2718
BtgI CCRYGG 4 cut(s) 574, 2282, 2755, 4370
BtrI CACGTC 2 cut(s) 2158, 2794
BtsI GCAGTG 5 cut(s) 125, 1786, 2593, 2642, 3979
BveI ACCTGC 6 cut(s) 333, 402, 712, 2318, 2902, 4406
Cac8I GCNNGC 7 cut(s) 571, 1398, 2499, 2779, 3521, 3591, 4587
CaiI CAGNNNCTG 3 cut(s) 1206, 3090, 3399
CfoI GCGC 1 cut(s) 4661
Cfr13I GGNCC 8 cut(s) 33, 1718, 1837, 1949, 2054, 3911, 4030, 4142
Cfr42I CCGCGG 1 cut(s) 577
Cfr9I CCCGGG 1 cut(s) 872
CseI GACGC 2 cut(s) 25, 936
Csp6I GTAC 6 cut(s) 308, 419, 823, 2841, 3010, 3804
CviQI GTAC 6 cut(s) 308, 419, 823, 2841, 3010, 3804
DraIII CACNNNGTG 2 cut(s) 483, 2646
DrdI GACNNNNNNGTC 1 cut(s) 2798
DseDI GACNNNNNNGTC 1 cut(s) 2798
EaeI YGGCCR 1 cut(s) 50
EagI CGGCCG 1 cut(s) 50
Eam1104I CTCTTC 9 cut(s) 457, 488, 1313, 1976, 2421, 2620, 2888, 3116, 4509
EarI CTCTTC 9 cut(s) 457, 488, 1313, 1976, 2421, 2620, 2888, 3116, 4509
EciI GGCGGA 3 cut(s) 175, 999, 3674
Ecl136II GAGCTC 3 cut(s) 778, 941, 2968
EclXI CGGCCG 1 cut(s) 50
Eco130I CCWWGG 6 cut(s) 273, 342, 1265, 3458, 3708, 4370
Eco24I GRGCYC 7 cut(s) 271, 340, 780, 943, 2461, 2970, 4549
Eco47I GGWCC 2 cut(s) 1718, 3911
Eco52I CGGCCG 1 cut(s) 50
Eco53kI GAGCTC 3 cut(s) 778, 941, 2968
Eco57I CTGAAG 3 cut(s) 1851, 2094, 4044
Eco81I CCTNAGG 1 cut(s) 2058
Eco88I CYCGRG 2 cut(s) 36, 872
EcoICRI GAGCTC 3 cut(s) 778, 941, 2968
EcoNI CCTNNNNNAGG 1 cut(s) 148
EcoRI GAATTC 1 cut(s) 66
EcoRII CCWGG 3 cut(s) 9, 143, 2118
EcoT14I CCWWGG 6 cut(s) 273, 342, 1265, 3458, 3708, 4370
EcoT22I ATGCAT 5 cut(s) 1052, 2175, 3248, 3544, 4263
EcoT38I GRGCYC 7 cut(s) 271, 340, 780, 943, 2461, 2970, 4549
ErhI CCWWGG 6 cut(s) 273, 342, 1265, 3458, 3708, 4370
FalI AAGNNNNNCTT 6 cut(s) 1824, 1856, 2361, 2393, 4017, 4049
FaqI GGGAC 5 cut(s) 855, 2117, 3042, 3726, 4205
FauI CCCGC 4 cut(s) 1389, 1927, 2937, 3582
FauNDI CATATG 1 cut(s) 59
FbaI TGATCA 2 cut(s) 2218, 4306
FblI GTMKAC 2 cut(s) 1646, 2695
FriOI GRGCYC 7 cut(s) 271, 340, 780, 943, 2461, 2970, 4549
FspBI CTAG 8 cut(s) 167, 318, 387, 1230, 2819, 3423, 3624, 4650
GlaI GCGC 1 cut(s) 4660
GsaI CCCAGC 3 cut(s) 1378, 3571, 3658
GsuI CTGGAG 5 cut(s) 2156, 2495, 2870, 4244, 4583
HapII CCGG 9 cut(s) 873, 907, 1603, 1709, 2113, 3094, 3729, 3796, 3902
HgaI GACGC 2 cut(s) 25, 936
HhaI GCGC 1 cut(s) 4661
Hin1I GRCGYC 1 cut(s) 17
Hin6I GCGC 1 cut(s) 4659
HinP1I GCGC 1 cut(s) 4659
HincII GTYRAC 1 cut(s) 1440
HindII GTYRAC 1 cut(s) 1440
HindIII AAGCTT 4 cut(s) 134, 1781, 3974, 4388
HpaII CCGG 9 cut(s) 873, 907, 1603, 1709, 2113, 3094, 3729, 3796, 3902
Hpy166II GTNNAC 9 cut(s) 477, 787, 951, 1440, 1647, 2286, 2640, 2696, 3153
Hpy8I GTNNAC 9 cut(s) 477, 787, 951, 1440, 1647, 2286, 2640, 2696, 3153
Hpy99I CGWCG 4 cut(s) 19, 25, 80, 83
HpyCH4III ACNGT 9 cut(s) 1524, 2227, 2239, 2858, 3326, 3440, 3607, 3717, 4315
HpyCH4IV ACGT 6 cut(s) 763, 1594, 2157, 2793, 2953, 4326
HpySE526I ACGT 6 cut(s) 763, 1594, 2157, 2793, 2953, 4326
Hsp92I GRCGYC 1 cut(s) 17
HspAI GCGC 1 cut(s) 4659
Kpn2I TCCGGA 1 cut(s) 2112
KpnI GGTACC 1 cut(s) 2844
Ksp22I TGATCA 2 cut(s) 2218, 4306
KspI CCGCGG 1 cut(s) 577
LguI GCTCTTC 1 cut(s) 457
MaeI CTAG 8 cut(s) 167, 318, 387, 1230, 2819, 3423, 3624, 4650
MaeII ACGT 6 cut(s) 763, 1594, 2157, 2793, 2953, 4326
MbiI CCGCTC 1 cut(s) 467
MfeI CAATTG 1 cut(s) 430
MflI RGATCY 1 cut(s) 2115
MlyI GAGTC 6 cut(s) 400, 1970, 2075, 2594, 4198, 4655
MmeI TCCRAC 4 cut(s) 1252, 1539, 2468, 4556
Mph1103I ATGCAT 5 cut(s) 1052, 2175, 3248, 3544, 4263
MroI TCCGGA 1 cut(s) 2112
MroXI GAANNNNTTC 2 cut(s) 2430, 4518
MspI CCGG 9 cut(s) 873, 907, 1603, 1709, 2113, 3094, 3729, 3796, 3902
MspR9I CCNGG 9 cut(s) 11, 145, 873, 874, 908, 1603, 2120, 3095, 3796
MunI CAATTG 1 cut(s) 430
Mva1269I GAATGC 1 cut(s) 3186
MvaI CCWGG 3 cut(s) 11, 145, 2120
MvnI CGCG 1 cut(s) 576
NciI CCSGG 6 cut(s) 873, 874, 908, 1603, 3095, 3796
NcoI CCATGG 1 cut(s) 4370
NdeI CATATG 1 cut(s) 59
NmeAIII GCCGAG 1 cut(s) 876
NmuCI GTSAC 8 cut(s) 764, 806, 1247, 1922, 2027, 2539, 3842, 4115
NsiI ATGCAT 5 cut(s) 1052, 2175, 3248, 3544, 4263
NspI RCATGY 9 cut(s) 1143, 2177, 2201, 2501, 3339, 4247, 4265, 4289, 4589
OliI CACNNNNGTG 3 cut(s) 1929, 2034, 4122
PaeI GCATGC 2 cut(s) 2501, 4589
PaqCI CACCTGC 2 cut(s) 712, 2902
PasI CCCWGGG 1 cut(s) 144
PciI ACATGT 2 cut(s) 1139, 3335
PciSI GCTCTTC 1 cut(s) 457
PctI GAATGC 1 cut(s) 3186
PdmI GAANNNNTTC 2 cut(s) 2430, 4518
PflFI GACNNNGTC 1 cut(s) 19
PflMI CCANNNNNTGG 2 cut(s) 1271, 3464
PfoI TCCNGGA 1 cut(s) 2118
PleI GAGTC 6 cut(s) 400, 1969, 2074, 2593, 4198, 4654
PpsI GAGTC 6 cut(s) 400, 1969, 2074, 2593, 4198, 4654
Ppu21I YACGTR 2 cut(s) 764, 2954
PscI ACATGT 2 cut(s) 1139, 3335
PshAI GACNNNNGTC 3 cut(s) 1716, 3909, 4379
PsiI TTATAA 2 cut(s) 3671, 3723
Psp124BI GAGCTC 3 cut(s) 780, 943, 2970
Psp6I CCWGG 3 cut(s) 9, 143, 2118
PspFI CCCAGC 3 cut(s) 1374, 3567, 3654
PspGI CCWGG 3 cut(s) 9, 143, 2118
PspPI GGNCC 8 cut(s) 33, 1718, 1837, 1949, 2054, 3911, 4030, 4142
PstI CTGCAG 6 cut(s) 330, 399, 839, 2329, 3026, 4417
PstNI CAGNNNCTG 3 cut(s) 1206, 3090, 3399
PsuI RGATCY 1 cut(s) 2115
PsyI GACNNNGTC 1 cut(s) 19
PvuII CAGCTG 9 cut(s) 688, 834, 1206, 1829, 2134, 2878, 3399, 4022, 4222
RsaI GTAC 6 cut(s) 309, 420, 824, 2842, 3011, 3805
RsaNI GTAC 6 cut(s) 308, 419, 823, 2841, 3010, 3804
SacI GAGCTC 3 cut(s) 780, 943, 2970
SacII CCGCGG 1 cut(s) 577
SapI GCTCTTC 1 cut(s) 457
Sau96I GGNCC 8 cut(s) 33, 1718, 1837, 1949, 2054, 3911, 4030, 4142
SbfI CCTGCAGG 2 cut(s) 2329, 4417
SchI GAGTC 6 cut(s) 400, 1970, 2075, 2594, 4198, 4655
ScrFI CCNGG 9 cut(s) 11, 145, 873, 874, 908, 1603, 2120, 3095, 3796
SdaI CCTGCAGG 2 cut(s) 2329, 4417
Sfr303I CCGCGG 1 cut(s) 577
SgrBI CCGCGG 1 cut(s) 577
SinI GGWCC 2 cut(s) 1718, 3911
SmaI CCCGGG 1 cut(s) 874
SphI GCATGC 2 cut(s) 2501, 4589
Sse8387I CCTGCAGG 2 cut(s) 2329, 4417
SspI AATATT 2 cut(s) 1813, 4006
SspMI CTAG 8 cut(s) 167, 318, 387, 1230, 2819, 3423, 3624, 4650
SstI GAGCTC 3 cut(s) 780, 943, 2970
StyD4I CCNGG 9 cut(s) 9, 143, 871, 872, 906, 1601, 2118, 3093, 3794
StyI CCWWGG 6 cut(s) 273, 342, 1265, 3458, 3708, 4370
TaaI ACNGT 9 cut(s) 1524, 2227, 2239, 2858, 3326, 3440, 3607, 3717, 4315
TaiI ACGT 6 cut(s) 766, 1597, 2160, 2796, 2956, 4329
TatI WGTACW 2 cut(s) 822, 3009
TauI GCSGC 4 cut(s) 52, 160, 576, 579
TseFI GTSAC 8 cut(s) 764, 806, 1247, 1922, 2027, 2539, 3842, 4115
Tsp45I GTSAC 8 cut(s) 764, 806, 1247, 1922, 2027, 2539, 3842, 4115
TspMI CCCGGG 1 cut(s) 872
Tth111I GACNNNGTC 1 cut(s) 19
Van91I CCANNNNNTGG 2 cut(s) 1271, 3464
VneI GTGCAC 2 cut(s) 475, 2638
VpaK11BI GGWCC 2 cut(s) 1718, 3911
XagI CCTNNNNNAGG 1 cut(s) 148
XapI RAATTY 8 cut(s) 66, 1278, 1583, 1616, 2165, 2267, 4253, 4355
XbaI TCTAGA 3 cut(s) 1229, 2818, 3422
XceI RCATGY 9 cut(s) 1143, 2177, 2201, 2501, 3339, 4247, 4265, 4289, 4589
XcmI CCANNNNNNNNNTGG 4 cut(s) 1389, 1502, 3582, 3695
XmaI CCCGGG 1 cut(s) 872
XmiI GTMKAC 2 cut(s) 1646, 2695
XmnI GAANNNNTTC 2 cut(s) 2430, 4518
XspI CTAG 8 cut(s) 167, 318, 387, 1230, 2819, 3423, 3624, 4650
Zsp2I ATGCAT 5 cut(s) 1052, 2175, 3248, 3544, 4263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.