Rroxscaffold_3G00244660

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000280
Physical Location & Seq
Reverse (-)
37495438 .. 37505539
10102 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00244660.1

Sequence Viewer

Length: 3222 bp
ATGGCGAGAGGGGGAGGCAGAGGAAGAGGGAGATGGACAAAGCGGCAAAGGGACCCAGGCCCAGAAACCCATTACCCGAATCAAACTGAAGATGTTGCTGTCAAAGTTGGTGAAGATGGTGATGGGGTTGTTCTTTCTGAGATAAAGGCTGTATCTTTTGGTGGTGTAGAGAAGGGTGAGGACAGTTTGGGAAATGATCAAAAGGAAGAAGTTGCTGTGAGTGTGAAGAAGAAGAAGAAGCCTGGGAGGAAGAGTAAGAAGGGTTTGGTTGGAAATGGTGATGGAGGTCTAAAGGAGGTTGCTTTTGGTGGTGATGAGATTGCTATGATGACTTTGAGGAAGAGGCCTGAACGGAACAAGAGAGTTAGAGTTATGGAGGCAGAGGAAAATGGTGAGAAAGGTGAAGAGAATGGTGCTTCTGTGAACAAGAAAGGTAGGAAAAAGGTGACACTTGCGAAGAAAGAGGGAAGGAAAGGTGTTTTAGAAGTGAATAATGACAAGAAGCGCGATAGGAAGCGTGCGAAAACTGAGGAGGGAGATGGGAATGAGCTAGGCTTCTCGTTTAGGCCTCTGAGGGAGCAGGGACATCTATCAATGGAGGAGCGTAAAAAGTGGATTGAGGAAGTATCCCTAATGTGCCACCAATGTCAGAGGAATGATAAGGGAAGGGTTGTTCGGTGCAAAAAGTGCAAGACAAAGCGATATTGTGCTCCTTGCATAGAGAATTGGTATCCACATACATCAGAGGATGCCATTGCTGAGGCTTGTCCTGTATGCCGTGGAAATTGCAATTGCAAAGGATGCTTGCGCTTAGATATACCAGTTAAAAATCTGAAGAACCAAGATTTAGTACTTGACAAAGATGCAAGAGTTGAGCACTCTAAGTACTTAATTCATGCACTGCTTCCATCCTTGAAAAGAATTCATGAAGAACAAGAGAATGAGATGGCGATGGAGGCAAGGAGAAAAGGATTATCTAACTTGATGCTCCAGATACCAAAGTCTGACTGTGCTGCTGATGTTCGCGTGTATTGCAACAACTGCAGAACTTCTATTTTTGATTATCACAGAAGCTGTCCTAAATGTTCATATGACCTCTGCCTCATTTGTTGCCGGGAGATTCGAGAAGGACAATTGCAGCTAGGTGGGGAGGAAGTGACTTTGGAGTATGTTTCTCAGGGCCTACCTTATTTGCATGGTGGAGAGGAAGAGGACAACAAAAAAAGAAAGGGAAAGAGAAATAGGAATGTTGTGGAGCCACCCTCTGAAACTAGTCCCAAAAGTTCTGGAAAGCAAACATTTGAGTGGAAATCATATGAAGATGGAAGCATACTTTGCCCCCAGAGGACATGGGAGGTTGTGGCAGTGAAAGCAGAAGAGATAGATGAAGATTACAAACTTGTGTATTCAAGTAGAACTTCTGCAGAGAGGTGTTCATGTCTCAACTCTGTCGATGATGTCAATTCAAGCATCAATGCATCAAGGAAAGCAGCTTCTCGAGACAATTCTGAGGACAACTATCTGTACTGTGCAAGGGCTGGAGATATCCAAGTAGAGGATTTAAAGCATTTCCAGTGGCATTGGATCAAGGGTGAGCCTGTTATCGTTAGCAATGCACTTGAAACTGCATCAGGTTTAAGCTGGGAACCATTGGTCATGTGGCGCGCTTGTCGTCAGATGCAACATACTAAGCATGGTAAACATTTGGACGTCAAAGCAATAGATTGCCTGGATTGGTGTGAGAACTTTAGCATGGCACAGAACACCTGTTTGGTAGCACTACATGCATGGCTGGGAACTTGTTCTCAATCTGATCTTAGGGGGGATATCAATATCCACCAATTTTTTACTGGATACTCGAAGGGACGGTTTGATAAAGAGAATTGGCCACAGATATTGAAACTGAAAGATTGGCCGCCAACTAATTTATTTGAGGAGCGCCTTCCTCGTCATGGTGCTGAGTTCATTTGTTGTCTGCCTTATAAGGAATATACTCATCCTCGCAATGGTCCTCTAAACCTTTTTGCCAAATTACCTTCAGAATGTGTAAAACCAGACATGGGACCAAAGACATATATTGCTTATGGTTTTTCCCAGGAGCTTGGACGTGGAGATTCTGTAACCAAGCTTCATTGTGATATGTCTGATGCGGGTGGCAGAGGTGGGTTTGGGGGCTTTCTGACCGAATTCTTTATGCGTTGGAGTCTTTACTTAAATATACATAGATTATCTAGTGTGGTCTCTGGTTTGAAGGTTGATATACTCGTGTTTATTGAGCATATAGTTGCACCAATTATAACATTAAGTGTGAATGTTTTGACACATACGACTGAAGTGAACCTTACTCCTGAGCAACTTACTGTCATTGAAAAGTTGAAAAAGAAGCATGCTCAACAAGACCAAAGAGAAATTTTTGGCAATTGTCAGACTGTGGATGATGATGTTGATAGTGGCAAGGCTGGTGGTGATTCTTGTTTCAGTGCTGCTGATGATAAACAACTTCTTAATGAAGTTGATAATAATAGCAGTGGTGTAGCTGTTCCAGAACTGGTTGATCCAATTGTTCAACATGTTGGAGATGCTTCAATGGGATCTGTGTTCGAAAAGAACAAAGAGGCAGAATTAGATCATGATATCGGTGGTGACATGCCTGCAAGAAATTATGGAAATAAGTTGGAAGAGTCAGATGTCAATGAGGGAGGTGCTTTATGGGACATATTCCGTAGACAGGATGTTCCTAAGCTGCAGGAGTATCTCATGAAGCATTTCAAGGAATTCAGGCACATACACTGTTGTCCATTGCAGCAGGTTATTCATCCCATACATGATCAGACATTTTATCTTACAATGGAGCACAAAAAGAAGCTTAAAGAGGAATATGGAATTGAACCATGGACCTTCATTCAAAACCTTGGAGATGCTGTCTTTATTCCTGCAGGATGTCCGCATCAAGTCAGGAACTTGAAGTCATGCATCAAAGTTGCAATGGACTTTGTCTCACCTGAAAATGTCGCCGAGTGTTTTCACTTGACAGAAGTATTCCGCACACTTCCAGAAAATCATAGGGCTAAAGAGGACAAATTGGAGGTAAAGAAAATGATTGTTCATTCTGTCACTGATGCTCTGGAGGAACTAAATCAGAATGTGAGGTCTAAGGAAGCCACTAATTCACCTCAATCAAGTCAGAGCGATGGAGATTATATGGTCCAGGATACTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1073

Amino Acids

121.32

Weight (kDa)

6.61

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 210 - 270 4.3e-07 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 901 - 998 8.4e-15 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1983, 2297
AatII GACGTC 1 cut(s) 1713
Acc36I ACCTGC 1 cut(s) 2797
AccI GTMKAC 1 cut(s) 2725
AccII CGCG 3 cut(s) 507, 1026, 1665
AciI CCGC 5 cut(s) 43, 1916, 2150, 2945, 3043
AclWI GGATC 3 cut(s) 1592, 2549, 2599
AcoI YGGCCR 2 cut(s) 1886, 1913
AcsI RAATTY 4 cut(s) 921, 2186, 2409, 2774
AcuI CTGAAG 4 cut(s) 108, 854, 2022, 2352
AcyI GRCGYC 1 cut(s) 1710
AfaI GTAC 3 cut(s) 852, 887, 1526
AfiI CCNNNNNNNGG 5 cut(s) 1555, 1952, 2006, 2060, 2728
AflIII ACRYGT 1 cut(s) 2569
AhlI ACTAGT 1 cut(s) 1271
AjiI CACGTC 1 cut(s) 2108
AjnI CCWGG 5 cut(s) 55, 241, 1728, 2094, 3207
AjuI GAANNNNNNNTTGG 2 cut(s) 1754, 1786
AloI GAACNNNNNNTCC 2 cut(s) 657, 689
Alw21I GWGCWC 3 cut(s) 712, 879, 2856
Alw26I GTCTC 4 cut(s) 1445, 1494, 2245, 3001
AlwI GGATC 3 cut(s) 1592, 2549, 2599
AlwNI CAGNNNCTG 1 cut(s) 1074
Ama87I CYCGRG 1 cut(s) 1497
AoxI GGCC 6 cut(s) 58, 344, 566, 1180, 1886, 1913
ApeKI GCWGC 6 cut(s) 1013, 1138, 1490, 2483, 2743, 2803
ApoI RAATTY 4 cut(s) 921, 2186, 2409, 2774
Asp700I GAANNNNTTC 2 cut(s) 1801, 2765
AspLEI GCGC 5 cut(s) 507, 810, 1665, 1667, 1941
AspS9I GGNCC 7 cut(s) 52, 59, 1180, 2009, 2063, 2895, 3205
AsuC2I CCSGG 1 cut(s) 1115
AsuII TTCGAA 1 cut(s) 2601
AvaI CYCGRG 1 cut(s) 1497
AvaII GGWCC 5 cut(s) 52, 2009, 2063, 2895, 3205
BaeI ACNNNNGTAYC 2 cut(s) 986, 1019
BalI TGGCCA 1 cut(s) 1888
BarI GAAGNNNNNNTAC 2 cut(s) 2112, 2144
BauI CACGAG 1 cut(s) 2264
Bbv12I GWGCWC 3 cut(s) 712, 879, 2856
BbvCI CCTCAGC 1 cut(s) 759
BbvI GCAGC 6 cut(s) 1000, 1150, 1502, 2470, 2730, 2815
BceAI ACGGC 1 cut(s) 762
BciT130I CCWGG 5 cut(s) 57, 243, 1730, 2096, 3209
BciVI GTATCC 4 cut(s) 637, 741, 1847, 3205
BclI TGATCA 2 cut(s) 196, 2827
BcnI CCSGG 1 cut(s) 1115
BcoDI GTCTC 4 cut(s) 1445, 1494, 2245, 3001
BcuI ACTAGT 1 cut(s) 1271
BfaI CTAG 4 cut(s) 551, 1142, 1272, 2232
BfmI CTRYAG 4 cut(s) 1042, 1422, 2744, 2934
BfoI RGCGCY 1 cut(s) 1942
BfuAI ACCTGC 1 cut(s) 2797
BfuI GTATCC 4 cut(s) 637, 741, 1847, 3205
BisI GCNGC 8 cut(s) 44, 1014, 1139, 1491, 1916, 2484, 2744, 2804
BlsI GCNGC 8 cut(s) 45, 1015, 1140, 1492, 1917, 2485, 2745, 2805
BmcAI AGTACT 2 cut(s) 852, 887
Bme1390I CCNGG 6 cut(s) 57, 243, 1115, 1730, 2096, 3209
Bme18I GGWCC 5 cut(s) 52, 2009, 2063, 2895, 3205
BmeT110I CYCGRG 1 cut(s) 1497
BmgBI CACGTC 1 cut(s) 2108
BmgT120I GGNCC 7 cut(s) 52, 59, 1180, 2009, 2063, 2895, 3205
BmiI GGNNCC 5 cut(s) 53, 54, 1257, 1647, 2064
BmrFI CCNGG 6 cut(s) 57, 243, 1115, 1730, 2096, 3209
BplI GAGNNNNNCTC 2 cut(s) 1247, 1279
BpmI CTGGAG 3 cut(s) 974, 1560, 3146
Bpu10I CCTNAGC 3 cut(s) 759, 2349, 2739
Bpu14I TTCGAA 1 cut(s) 2601
BpuMI CCSGG 1 cut(s) 1115
BsaHI GRCGYC 1 cut(s) 1710
BsaI GGTCTC 1 cut(s) 2245
BsaJI CCNNGG 6 cut(s) 55, 242, 778, 2094, 2891, 2911
BsaXI ACNNNNNCTCC 6 cut(s) 238, 268, 2103, 2133, 2907, 2937
Bsc4I CCNNNNNNNGG 5 cut(s) 1555, 1952, 2006, 2060, 2728
Bse1I ACTGG 4 cut(s) 821, 1573, 1855, 2553
Bse3DI GCAATG 5 cut(s) 753, 1618, 2011, 2798, 2991
BseBI CCWGG 5 cut(s) 57, 243, 1730, 2096, 3209
BseDI CCNNGG 6 cut(s) 55, 242, 778, 2094, 2891, 2911
BseGI GGATG 8 cut(s) 754, 806, 908, 1996, 2440, 2737, 2815, 2945
BseLI CCNNNNNNNGG 5 cut(s) 1555, 1952, 2006, 2060, 2728
BseMI GCAATG 5 cut(s) 753, 1618, 2011, 2798, 2991
BseMII CTCAG 8 cut(s) 129, 519, 563, 750, 1190, 1500, 1950, 2340
BseNI ACTGG 4 cut(s) 821, 1573, 1855, 2553
BsePI GCGCGC 1 cut(s) 1663
BseRI GAGGAG 3 cut(s) 545, 614, 1949
BseXI GCAGC 6 cut(s) 1000, 1150, 1502, 2470, 2730, 2815
BseYI CCCAGC 2 cut(s) 1641, 1792
Bsh1236I CGCG 3 cut(s) 507, 1026, 1665
BshFI GGCC 6 cut(s) 60, 346, 568, 1182, 1888, 1915
BsiHKAI GWGCWC 3 cut(s) 712, 879, 2856
BsiHKCI CYCGRG 1 cut(s) 1497
BsiSI CCGG 1 cut(s) 1114
BslFI GGGAC 6 cut(s) 65, 597, 1260, 1878, 2076, 2726
BslI CCNNNNNNNGG 5 cut(s) 1555, 1952, 2006, 2060, 2728
BsmAI GTCTC 4 cut(s) 1445, 1494, 2245, 3001
BsmFI GGGAC 6 cut(s) 65, 597, 1260, 1878, 2076, 2726
BsnI GGCC 6 cut(s) 60, 346, 568, 1182, 1888, 1915
Bso31I GGTCTC 1 cut(s) 2245
BsoBI CYCGRG 1 cut(s) 1497
Bsp119I TTCGAA 1 cut(s) 2601
Bsp1286I GDGCHC 3 cut(s) 712, 879, 2856
Bsp143I GATC 7 cut(s) 196, 1584, 1813, 2554, 2591, 2626, 2827
Bsp19I CCATGG 1 cut(s) 2891
BspACI CCGC 5 cut(s) 43, 1916, 2150, 2945, 3043
BspANI GGCC 6 cut(s) 60, 346, 568, 1182, 1888, 1915
BspCNI CTCAG 8 cut(s) 130, 520, 564, 751, 1189, 1501, 1951, 2341
BspFNI CGCG 3 cut(s) 507, 1026, 1665
BspHI TCATGA 3 cut(s) 925, 2629, 2757
BspLI GGNNCC 5 cut(s) 53, 54, 1257, 1647, 2064
BspMAI CTGCAG 4 cut(s) 1046, 1426, 2748, 2938
BspMI ACCTGC 1 cut(s) 2797
BspPI GGATC 3 cut(s) 1592, 2549, 2599
BspT104I TTCGAA 1 cut(s) 2601
BspTNI GGTCTC 1 cut(s) 2245
BsrDI GCAATG 5 cut(s) 753, 1618, 2011, 2798, 2991
BsrI ACTGG 4 cut(s) 821, 1573, 1855, 2553
BssECI CCNNGG 6 cut(s) 55, 242, 778, 2094, 2891, 2911
BssHII GCGCGC 1 cut(s) 1663
BssMI GATC 7 cut(s) 196, 1584, 1813, 2554, 2591, 2626, 2827
BssNI GRCGYC 1 cut(s) 1710
BssSI CACGAG 1 cut(s) 2264
BssT1I CCWWGG 2 cut(s) 2891, 2911
Bst2BI CACGAG 1 cut(s) 2264
Bst2UI CCWGG 5 cut(s) 57, 243, 1730, 2096, 3209
Bst4CI ACNGT 7 cut(s) 185, 1010, 1529, 1869, 2362, 2431, 2792
Bst6I CTCTTC 7 cut(s) 19, 245, 335, 399, 1203, 1371, 2673
BstACI GRCGYC 1 cut(s) 1710
BstAPI GCANNNNNTGC 5 cut(s) 687, 801, 1041, 1335, 1784
BstBI TTCGAA 1 cut(s) 2601
BstC8I GCNNGC 5 cut(s) 519, 806, 1665, 2388, 2652
BstDSI CCRYGG 2 cut(s) 778, 2891
BstF5I GGATG 8 cut(s) 754, 806, 908, 1996, 2440, 2737, 2815, 2945
BstFNI CGCG 3 cut(s) 507, 1026, 1665
BstH2I RGCGCY 1 cut(s) 1942
BstHHI GCGC 5 cut(s) 507, 810, 1665, 1667, 1941
BstKTI GATC 7 cut(s) 199, 1587, 1816, 2557, 2594, 2629, 2830
BstMAI GTCTC 4 cut(s) 1445, 1494, 2245, 3001
BstMBI GATC 7 cut(s) 196, 1584, 1813, 2554, 2591, 2626, 2827
BstMWI GCNNNNNNNGC 8 cut(s) 687, 801, 956, 1032, 1041, 1335, 1370, 1784
BstNI CCWGG 5 cut(s) 57, 243, 1730, 2096, 3209
BstNSI RCATGY 4 cut(s) 1787, 2390, 2573, 2650
BstSCI CCNGG 6 cut(s) 55, 241, 1113, 1728, 2094, 3207
BstSFI CTRYAG 4 cut(s) 1042, 1422, 2744, 2934
BstUI CGCG 3 cut(s) 507, 1026, 1665
BstV1I GCAGC 6 cut(s) 1000, 1150, 1502, 2470, 2730, 2815
BstX2I RGATCY 1 cut(s) 2591
BstXI CCANNNNNNTGG 1 cut(s) 2102
BstYI RGATCY 1 cut(s) 2591
BsuI GTATCC 4 cut(s) 637, 741, 1847, 3205
BsuRI GGCC 6 cut(s) 60, 346, 568, 1182, 1888, 1915
BtgI CCRYGG 2 cut(s) 778, 2891
BtgZI GCGATG 2 cut(s) 965, 3204
BtrI CACGTC 1 cut(s) 2108
BtsCI GGATG 8 cut(s) 754, 806, 908, 1996, 2440, 2737, 2815, 2945
BtsI GCAGTG 3 cut(s) 899, 1371, 2533
BtsIMutI CAGTG 7 cut(s) 899, 1371, 1580, 2485, 2533, 2788, 3114
BveI ACCTGC 1 cut(s) 2797
Cac8I GCNNGC 5 cut(s) 519, 806, 1665, 2388, 2652
CaiI CAGNNNCTG 1 cut(s) 1074
CciI TCATGA 3 cut(s) 925, 2629, 2757
CfoI GCGC 5 cut(s) 507, 810, 1665, 1667, 1941
Cfr13I GGNCC 7 cut(s) 52, 59, 1180, 2009, 2063, 2895, 3205
Csp6I GTAC 3 cut(s) 851, 886, 1525
CspCI CAANNNNNGTGG 4 cut(s) 1878, 1913, 2443, 2478
CviQI GTAC 3 cut(s) 851, 886, 1525
DpnI GATC 7 cut(s) 198, 1586, 1815, 2556, 2593, 2628, 2829
DpnII GATC 7 cut(s) 196, 1584, 1813, 2554, 2591, 2626, 2827
DraI TTTAAA 1 cut(s) 1563
EaeI YGGCCR 2 cut(s) 1886, 1913
Eam1104I CTCTTC 7 cut(s) 19, 245, 335, 399, 1203, 1371, 2673
EarI CTCTTC 7 cut(s) 19, 245, 335, 399, 1203, 1371, 2673
Eco130I CCWWGG 2 cut(s) 2891, 2911
Eco147I AGGCCT 2 cut(s) 346, 568
Eco31I GGTCTC 1 cut(s) 2245
Eco32I GATATC 3 cut(s) 1546, 1828, 2635
Eco47I GGWCC 5 cut(s) 52, 2009, 2063, 2895, 3205
Eco57I CTGAAG 4 cut(s) 108, 854, 2022, 2352
Eco88I CYCGRG 1 cut(s) 1497
EcoO109I RGGNCCY 2 cut(s) 52, 1180
EcoRI GAATTC 3 cut(s) 921, 2186, 2774
EcoRII CCWGG 5 cut(s) 55, 241, 1728, 2094, 3207
EcoRV GATATC 3 cut(s) 1546, 1828, 2635
EcoT14I CCWWGG 2 cut(s) 2891, 2911
EcoT22I ATGCAT 3 cut(s) 1480, 1789, 2975
ErhI CCWWGG 2 cut(s) 2891, 2911
FalI AAGNNNNNCTT 4 cut(s) 1402, 1434, 2325, 2357
FaqI GGGAC 6 cut(s) 65, 597, 1260, 1878, 2076, 2726
FauI CCCGC 1 cut(s) 2143
FauNDI CATATG 2 cut(s) 1090, 1315
FbaI TGATCA 2 cut(s) 196, 2827
FblI GTMKAC 1 cut(s) 2725
Fnu4HI GCNGC 8 cut(s) 44, 1014, 1139, 1491, 1916, 2484, 2744, 2804
FokI GGATG 8 cut(s) 761, 813, 895, 1983, 2447, 2744, 2802, 2952
Fsp4HI GCNGC 8 cut(s) 44, 1014, 1139, 1491, 1916, 2484, 2744, 2804
FspBI CTAG 4 cut(s) 551, 1142, 1272, 2232
GlaI GCGC 5 cut(s) 506, 809, 1664, 1666, 1940
GluI GCNGC 8 cut(s) 44, 1014, 1139, 1491, 1916, 2484, 2744, 2804
GsaI CCCAGC 2 cut(s) 1645, 1796
GsuI CTGGAG 3 cut(s) 974, 1560, 3146
HaeII RGCGCY 1 cut(s) 1942
HaeIII GGCC 6 cut(s) 60, 346, 568, 1182, 1888, 1915
HapII CCGG 1 cut(s) 1114
HhaI GCGC 5 cut(s) 507, 810, 1665, 1667, 1941
Hin1I GRCGYC 1 cut(s) 1710
Hin6I GCGC 5 cut(s) 505, 808, 1663, 1665, 1939
HinP1I GCGC 5 cut(s) 505, 808, 1663, 1665, 1939
HindIII AAGCTT 2 cut(s) 2126, 2864
HinfI GANTC 6 cut(s) 79, 1120, 2114, 2203, 2468, 2681
HpaII CCGG 1 cut(s) 1114
Hpy166II GTNNAC 4 cut(s) 424, 1700, 2338, 2726
Hpy8I GTNNAC 4 cut(s) 424, 1700, 2338, 2726
HpyCH4III ACNGT 7 cut(s) 185, 1010, 1529, 1869, 2362, 2431, 2792
HpyCH4IV ACGT 2 cut(s) 1710, 2107
HpyF10VI GCNNNNNNNGC 8 cut(s) 687, 801, 956, 1032, 1041, 1335, 1370, 1784
HpySE526I ACGT 2 cut(s) 1710, 2107
Hsp92I GRCGYC 1 cut(s) 1710
HspAI GCGC 5 cut(s) 505, 808, 1663, 1665, 1939
KflI GGGWCCC 1 cut(s) 52
Ksp22I TGATCA 2 cut(s) 196, 2827
Kzo9I GATC 7 cut(s) 196, 1584, 1813, 2554, 2591, 2626, 2827
LmnI GCTCC 8 cut(s) 577, 601, 715, 993, 1255, 1936, 2098, 2851
Lsp1109I GCAGC 6 cut(s) 1000, 1150, 1502, 2470, 2730, 2815
MaeI CTAG 4 cut(s) 551, 1142, 1272, 2232
MaeII ACGT 2 cut(s) 1710, 2107
MaeIII GTNAC 5 cut(s) 445, 1156, 2119, 2642, 3112
MalI GATC 7 cut(s) 198, 1586, 1815, 2556, 2593, 2628, 2829
MboI GATC 7 cut(s) 196, 1584, 1813, 2554, 2591, 2626, 2827
MfeI CAATTG 4 cut(s) 790, 1133, 2419, 2559
MflI RGATCY 1 cut(s) 2591
MhlI GDGCHC 3 cut(s) 712, 879, 2856
MlsI TGGCCA 1 cut(s) 1888
MluNI TGGCCA 1 cut(s) 1888
MlyI GAGTC 2 cut(s) 2212, 2690
MmeI TCCRAC 4 cut(s) 250, 2180, 2554, 2655
Mox20I TGGCCA 1 cut(s) 1888
Mph1103I ATGCAT 3 cut(s) 1480, 1789, 2975
MroXI GAANNNNTTC 2 cut(s) 1801, 2765
MscI TGGCCA 1 cut(s) 1888
MseI TTAA 8 cut(s) 825, 890, 1562, 1637, 2213, 2303, 2505, 2868
MslI CAYNNNNRTG 1 cut(s) 1303
Msp20I TGGCCA 1 cut(s) 1888
MspI CCGG 1 cut(s) 1114
MspR9I CCNGG 6 cut(s) 57, 243, 1115, 1730, 2096, 3209
MunI CAATTG 4 cut(s) 790, 1133, 2419, 2559
MvaI CCWGG 5 cut(s) 57, 243, 1730, 2096, 3209
MvnI CGCG 3 cut(s) 507, 1026, 1665
MwoI GCNNNNNNNGC 8 cut(s) 687, 801, 956, 1032, 1041, 1335, 1370, 1784
NciI CCSGG 1 cut(s) 1115
NcoI CCATGG 1 cut(s) 2891
NdeI CATATG 2 cut(s) 1090, 1315
NdeII GATC 7 cut(s) 196, 1584, 1813, 2554, 2591, 2626, 2827
NlaIV GGNNCC 5 cut(s) 53, 54, 1257, 1647, 2064
NmeAIII GCCGAG 1 cut(s) 3040
NmuCI GTSAC 4 cut(s) 445, 1156, 2642, 3112
NsiI ATGCAT 3 cut(s) 1480, 1789, 2975
NspI RCATGY 4 cut(s) 1787, 2390, 2573, 2650
NspV TTCGAA 1 cut(s) 2601
PaeI GCATGC 1 cut(s) 2390
PaeR7I CTCGAG 1 cut(s) 1497
PagI TCATGA 3 cut(s) 925, 2629, 2757
PauI GCGCGC 1 cut(s) 1663
PceI AGGCCT 2 cut(s) 346, 568
PciI ACATGT 1 cut(s) 2569
PdmI GAANNNNTTC 2 cut(s) 1801, 2765
PfeI GAWTC 4 cut(s) 79, 1120, 2114, 2468
PflFI GACNNNGTC 1 cut(s) 2993
PfoI TCCNGGA 1 cut(s) 3207
PkrI GCNGC 8 cut(s) 45, 1015, 1140, 1492, 1917, 2485, 2745, 2805
PleI GAGTC 2 cut(s) 2211, 2689
PpsI GAGTC 2 cut(s) 2211, 2689
PpuMI RGGWCCY 1 cut(s) 52
PscI ACATGT 1 cut(s) 2569
PsiI TTATAA 2 cut(s) 1983, 2297
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 5 cut(s) 55, 241, 1728, 2094, 3207
PspFI CCCAGC 2 cut(s) 1641, 1792
PspGI CCWGG 5 cut(s) 55, 241, 1728, 2094, 3207
PspN4I GGNNCC 5 cut(s) 53, 54, 1257, 1647, 2064
PspPI GGNCC 7 cut(s) 52, 59, 1180, 2009, 2063, 2895, 3205
PspPPI RGGWCCY 1 cut(s) 52
PstI CTGCAG 4 cut(s) 1046, 1426, 2748, 2938
PstNI CAGNNNCTG 1 cut(s) 1074
PsuI RGATCY 1 cut(s) 2591
PsyI GACNNNGTC 1 cut(s) 2993
PteI GCGCGC 1 cut(s) 1663
RsaI GTAC 3 cut(s) 852, 887, 1526
RsaNI GTAC 3 cut(s) 851, 886, 1525
RseI CAYNNNNRTG 1 cut(s) 1303
SaqAI TTAA 8 cut(s) 825, 890, 1562, 1637, 2213, 2303, 2505, 2868
SatI GCNGC 8 cut(s) 44, 1014, 1139, 1491, 1916, 2484, 2744, 2804
Sau3AI GATC 7 cut(s) 196, 1584, 1813, 2554, 2591, 2626, 2827
Sau96I GGNCC 7 cut(s) 52, 59, 1180, 2009, 2063, 2895, 3205
SbfI CCTGCAGG 1 cut(s) 2938
ScaI AGTACT 2 cut(s) 852, 887
SchI GAGTC 2 cut(s) 2212, 2690
ScrFI CCNGG 6 cut(s) 57, 243, 1115, 1730, 2096, 3209
SdaI CCTGCAGG 1 cut(s) 2938
SduI GDGCHC 3 cut(s) 712, 879, 2856
SfcI CTRYAG 4 cut(s) 1042, 1422, 2744, 2934
Sfr274I CTCGAG 1 cut(s) 1497
SfuI TTCGAA 1 cut(s) 2601
SinI GGWCC 5 cut(s) 52, 2009, 2063, 2895, 3205
SlaI CTCGAG 1 cut(s) 1497
SmiMI CAYNNNNRTG 1 cut(s) 1303
SmlI CTYRAG 1 cut(s) 1497
SmoI CTYRAG 1 cut(s) 1497
SpeI ACTAGT 1 cut(s) 1271
SphI GCATGC 1 cut(s) 2390
Sse8387I CCTGCAGG 1 cut(s) 2938
SseBI AGGCCT 2 cut(s) 346, 568
SsiI CCGC 5 cut(s) 43, 1916, 2150, 2945, 3043
SspMI CTAG 4 cut(s) 551, 1142, 1272, 2232
StuI AGGCCT 2 cut(s) 346, 568
StyD4I CCNGG 6 cut(s) 55, 241, 1113, 1728, 2094, 3207
StyI CCWWGG 2 cut(s) 2891, 2911
TaaI ACNGT 7 cut(s) 185, 1010, 1529, 1869, 2362, 2431, 2792
TaiI ACGT 2 cut(s) 1713, 2110
TaqI TCGA 5 cut(s) 1123, 1452, 1498, 1859, 2601
TaqII GACCGA 1 cut(s) 2198
TatI WGTACW 3 cut(s) 850, 885, 1524
TauI GCSGC 2 cut(s) 46, 1918
TfiI GAWTC 4 cut(s) 79, 1120, 2114, 2468
Tru1I TTAA 8 cut(s) 825, 890, 1562, 1637, 2213, 2303, 2505, 2868
Tru9I TTAA 8 cut(s) 825, 890, 1562, 1637, 2213, 2303, 2505, 2868
TscAI CASTG 7 cut(s) 906, 1371, 1580, 2485, 2533, 2795, 3121
TseFI GTSAC 4 cut(s) 445, 1156, 2642, 3112
TseI GCWGC 6 cut(s) 1013, 1138, 1490, 2483, 2743, 2803
Tsp45I GTSAC 4 cut(s) 445, 1156, 2642, 3112
TspGWI ACGGA 2 cut(s) 367, 2711
TspRI CASTG 7 cut(s) 906, 1371, 1580, 2485, 2533, 2795, 3121
Tth111I GACNNNGTC 1 cut(s) 2993
VpaK11BI GGWCC 5 cut(s) 52, 2009, 2063, 2895, 3205
XapI RAATTY 4 cut(s) 921, 2186, 2409, 2774
XceI RCATGY 4 cut(s) 1787, 2390, 2573, 2650
XcmI CCANNNNNNNNNTGG 2 cut(s) 1656, 1847
XhoI CTCGAG 1 cut(s) 1497
XmiI GTMKAC 1 cut(s) 2725
XmnI GAANNNNTTC 2 cut(s) 1801, 2765
XspI CTAG 4 cut(s) 551, 1142, 1272, 2232
ZraI GACGTC 1 cut(s) 1711
ZrmI AGTACT 2 cut(s) 852, 887
Zsp2I ATGCAT 3 cut(s) 1480, 1789, 2975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.