Rh7BG355800

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000280
Physical Location & Seq
Forward (+)
37290454 .. 37295393
4940 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG355800.1

Sequence Viewer

Length: 4638 bp
ATGAGAATCCAGGCGACGCCGGCGAGATTGTGGCCCCGAGGCACAAAGCGGCCGAACATATGGGGAATTCAGAACGACGACGGAACAAATGGGGAAGATGGTGGAGGACCGTCTGTGGAGCCACTGCCAAAGCTTGTTCCCTGGGGGAGGAAGCGGCGGAGGCTAGGATGCGAGGGTTTTTGGGCAAATCAAGATGAAGGAAAGGCAAGGGCTGTGAAGGCGAAGAAGAAGAAGAAGAAGCAGCAGCAGCAGCAGCAGCAGAGAAGGGCTCCCTTGGAGGTGCAGCAGCAGACAAGGGATGAACAAGTACCACTACCTAGCTACCTGCAGAGAAGGGCTCCCTTGAAGGTACGGACAATCAAGTGCCATCAATGTAAGCAAAATGATGTGGGAAGAGCGGTGCGGTGCACAAAGTGTTCGAGGAAGAGGTATTGTATCCCTTGCATAAGGACTTGGTATCCCAACACCTCAGAGGAAGCCATAGCTGAGGCTTGTCCTGCGTGCCGCGGCAACTGCAACTGCAATGTCTGCCTTTGTTGGGGTGTGCCTGTCAGGAATCTGAACCCGAAAATCGACAAGGAGACCAAGCTGGAGTACTCTAAGTATTTGGTTCATCAGCTGCTTCCTTATTTGAAGATGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTGGAAGCCAAGAGGCAGGGCTTACGTGACCTTTCGGAGCTCAAGGTGGACAAGGCTAAACGCGGTGACCAAGTGCGTATGTACTGCAAGAGCTGCAGCACTTCGATTTTTGATTTTCATAGGACTTGTCCCGGGTGTGAGTATGATCTGTGCCTCGGTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAAGAAGAGAGCGGAAGTGTTCTCATTGGAGTGGAGAGCAACTGAAGACGGAAACATTTTCTGCCCACCAACACACATGAAAGGTTGTGGTAAACATAATTTAGAATTGAGATGCATTTTTCCTCATAATCATGTCATGGAGTTGGTAGAGAAAGCAGAAGAAATAGATGCAAGTTACAATTTTTTGCCTGATGGTAGTGAAACATGTGCATATAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTCAAGTAGCAGCAGCTGTAAGTTAAGGAAAGCAGCTTCTAGAGTTGCTTCTGATAGTGACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAAGATAAGGATTTTGGGCATTTTCAGTGGCATTGGAAGAGGGGCGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCTGTCATGTGGCGGGCATTTAGACAGATAGAGAAAGTCAAGAATGATAGTGCATTGACATTCCACGCCATTGATTGCTGGGATTGGTGTTTTATAGAAATCAACATCCTCCATTTCTTTAGTGGATACTCCAACGGACGGTCTTATAAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATCATCTGCTGAGTTTTACAAACGTCTTCCCCGGCATTGTAAGGAATTTTTATGTTGTCTGCCTCTCAAGGAGTATACTCACCCCAGCAAGGGAGCTCTGAACCTTGCTATCCACTTGCCACCTACCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACAGGGAGATTCTGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACAACTGAACAACTTGCAACTATAGAGAAGTTGAAGAAAAAGCACAGGGAGCAAAACGAAGGAGAGATATTTGGGAATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCTCAGCAAGAGTCAGCTATAGAGAAGTTGAGGAGAAAGCACAGGGAGCAAAACGGAAGAGAGATATTTGGGAATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCTCAGCAAGAGTCAGCTGAAGTATGTGAGGATGGAAGTGCCTTATGGGACATTTTCCGGATCCAGGATGTTCCACAGCTGGAGAAATACATCCACAAGCATGTCAATGAATTTATGCATGTACTTGGTAATCCTTTGCAGCATGTTACTCATGCTATACATGATCAGACTGTCTATCTCACTGTGGAACACAAGAGGAAGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTGGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACAAACCACAGAGCCCATGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTATGAAGGAGATTTCTGGTGAGAATGTGACAAAGAGGGTAAGGGCTGTGAAGGCAAAGAGGGCAAGGACTCGTCCCCTGGACGAGTCGATACACTGCCATCAGTGTCATAGATATGTTATGGGAAGAGTGGTGCAGTGCACAAGGTGTCCGGAAAGAGGTACGGGAATGAGGACGAGGTATTGTATCCCTTGTATACAGAGATGGTATCCCAACACATCAGAGGAAGCCATTGCTAAGGCTTGTCCTGTATGCCACGGCAACTGCAACTGCAGAGCCTGCCTTCGTTTGGATGTGCCTGTCAGGCGTTTGCAGAATCTAGACCCCAAAATCGACAGGGGTACCAAGCTGGAGCACTGTAAGTATTTGGTTCATCAGCTGCTTCCCTATTTGAAGAGGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTCGAGGCTAAGAGGCAGGGATTACGTGATGTTTTGGAGCTCAAGGTGGAAAAGGCTAAGTGCGGTGAAGATGAGCGTATGTACTGTAACAACTGCAGAACTTCGATTTTTGATTTTCATAGGAGTTGTCCCAAGTGTGAGTATGATCTGTGCCTCAGTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAAGAAGAGAGAGGTGGTGGAGGAAGCTCAAGTGTCCACATCTGAGTGGAGATTTAGTGAAGATGGAAGCATTCTCTGCCTCCCAACACATAAGAAATGTTTTGGTAAGCATAATTTAGAATTGAGATGCATTTTTTCTCAGAATCATGTCATGGAATTGGTCGAGAAAGCAGGAAAAATAGATGCAAGTTACAATTTTTTGCCTGATCGTAGTGAAACATGTGCATACAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTCAAGCAGCAGCTGCTGTAAGTTAAGGAAAGCAGCTTCTAGAGTTGCTTCTGATAGTAACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAAGATAAGGATTTTGGGCATTTTCAGTGGCATTGGATGAGGGGCGAGCCTGTGGTCGTTAGCAATGCATTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCTGTCATGTGGCGGGCATTTAGAAAGGTAGATAATAGCTACGATGGTAGTGAATTGGCAGTCCACGCCGTTGATTGCTGGGATTGGTGTTTTATAAGAATCAAAATCCGCCATTTCTTTAGTGGATATTCCAACGGACAGTCTTATAAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATCATCTACTGAGTTTAACAATCGTCTTCCCCGGCATGGTAGGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATAGTCACCCCAACAAGGGGACTCTGAACCTTGCTATCTACTTGCCTCCTCGCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACAGGGAGATTCTGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACAACTGAACAACTTGCAACTATAGAGAAGTGGAAGAAAAAGCACAGGGAGCAAGACAAAAGAAAGATATTTGGGAATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCTCAGCAAGAGTCAGCTGAAGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGACTCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTATGCATGTGCTCTGTAATCCTTTGCAGCATGTTACTCATGGTATACATGATCAGACTGTTTATCTCACTGTGGAGCACAAGAGGAGGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTGTCAAAGTTGCACTGGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACAAACCACAGAGCCAGTGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTCTGAAGGAGATTTCTGGTGAGAATGTGATGACTGAGAATGCAAAGCCTAGGGGGGGCGCAAGGGAATTAAGGCAGGGTGTAAGGCCCAAATCTTACAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1545

Amino Acids

176.73

Weight (kDa)

8.78

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 119 - 178 7.5e-07 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 665 - 768 2.9e-13 JmjC domain, hydroxylase
zf-4CXXC_R1 PF10497 840 - 906 1.1e-06 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 1371 - 1468 5.7e-13 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1449, 3602, 3654
AarI CACCTGC 2 cut(s) 643, 2833
Acc36I ACCTGC 5 cut(s) 333, 643, 2237, 2833, 4337
Acc65I GGTACC 1 cut(s) 2771
AccB1I GGYRCC 2 cut(s) 2771, 4112
AccB7I CCANNNNNTGG 2 cut(s) 1190, 3395
AccBSI CCGCTC 2 cut(s) 398, 869
AccI GTMKAC 3 cut(s) 1565, 2626, 4231
AccII CGCG 2 cut(s) 507, 732
AccIII TCCGGA 3 cut(s) 2031, 2581, 4131
AclWI GGATC 4 cut(s) 1288, 2029, 2042, 3493
AcoI YGGCCR 1 cut(s) 50
AcsI RAATTY 7 cut(s) 66, 1535, 2084, 2186, 3740, 4184, 4286
AcuI CTGAAG 6 cut(s) 921, 1770, 2013, 3975, 4113, 4556
AcyI GRCGYC 1 cut(s) 17
AdeI CACNNNGTG 2 cut(s) 414, 2577
AfaI GTAC 8 cut(s) 309, 351, 596, 752, 2097, 2593, 2773, 2942
AflIII ACRYGT 2 cut(s) 1061, 3266
AgsI TTSAA 9 cut(s) 346, 634, 1274, 1795, 2275, 2824, 3479, 3763, 4375
AjiI CACGTC 1 cut(s) 4177
AjnI CCWGG 4 cut(s) 9, 140, 2037, 2508
AleI CACNNNNGTG 3 cut(s) 1848, 1953, 4053
Alw21I GWGCWC 8 cut(s) 410, 711, 1588, 2573, 2787, 2901, 4200, 4266
Alw26I GTCTC 1 cut(s) 575
Alw44I GTGCAC 2 cut(s) 406, 2569
AlwI GGATC 4 cut(s) 1288, 2029, 2042, 3493
AlwNI CAGNNNCTG 5 cut(s) 1125, 2709, 3021, 3327, 3330
Ama87I CYCGRG 2 cut(s) 36, 800
Aor13HI TCCGGA 3 cut(s) 2031, 2581, 4131
ApaLI GTGCAC 2 cut(s) 406, 2569
ApoI RAATTY 7 cut(s) 66, 1535, 2084, 2186, 3740, 4184, 4286
Asp700I GAANNNNTTC 2 cut(s) 2349, 4449
Asp718I GGTACC 1 cut(s) 2771
AspA2I CCTAGG 1 cut(s) 4580
AspLEI GCGC 1 cut(s) 4592
AsuC2I CCSGG 6 cut(s) 801, 802, 836, 1522, 3026, 3727
AvaI CYCGRG 2 cut(s) 36, 800
AvaII GGWCC 3 cut(s) 107, 1637, 3842
AvrII CCTAGG 1 cut(s) 4580
BaeGI GKGCMC 2 cut(s) 410, 2573
BaeI ACNNNNGTAYC 2 cut(s) 2755, 2788
BamHI GGATCC 1 cut(s) 2034
BanI GGYRCC 2 cut(s) 2771, 4112
BanII GRGCYC 6 cut(s) 271, 340, 711, 1588, 2380, 2901
BarI GAAGNNNNNNTAC 4 cut(s) 1686, 1718, 3891, 3923
BbsI GAAGAC 3 cut(s) 909, 1508, 3713
Bbv12I GWGCWC 8 cut(s) 410, 711, 1588, 2573, 2787, 2901, 4200, 4266
BbvCI CCTCAGC 4 cut(s) 486, 1872, 1977, 4077
BceAI ACGGC 6 cut(s) 1088, 2185, 2704, 3293, 3560, 4285
BciT130I CCWGG 4 cut(s) 11, 142, 2039, 2510
BciVI GTATCC 5 cut(s) 446, 468, 1421, 2627, 2649
BclI TGATCA 2 cut(s) 2137, 4237
BcnI CCSGG 6 cut(s) 801, 802, 836, 1522, 3026, 3727
BcoDI GTCTC 1 cut(s) 575
BfaI CTAG 6 cut(s) 164, 318, 1149, 2750, 3354, 4581
BfmI CTRYAG 9 cut(s) 326, 763, 1782, 1887, 2244, 2701, 2953, 3987, 4344
BfuAI ACCTGC 5 cut(s) 333, 643, 2237, 2833, 4337
BfuI GTATCC 5 cut(s) 446, 468, 1421, 2627, 2649
BglI GCCNNNNNGGC 1 cut(s) 2734
BlnI CCTAGG 1 cut(s) 4580
BmcAI AGTACT 1 cut(s) 596
Bme18I GGWCC 3 cut(s) 107, 1637, 3842
BmeT110I CYCGRG 2 cut(s) 36, 800
BmgBI CACGTC 1 cut(s) 4177
BoxI GACNNNNGTC 4 cut(s) 1635, 2210, 3840, 4310
BpiI GAAGAC 3 cut(s) 909, 1508, 3713
BplI GAGNNNNNCTC 4 cut(s) 253, 285, 322, 354
BpmI CTGGAG 6 cut(s) 611, 2075, 2414, 2801, 4175, 4514
Bpu10I CCTNAGC 5 cut(s) 486, 1872, 1977, 2667, 4077
BpuMI CCSGG 6 cut(s) 801, 802, 836, 1522, 3026, 3727
BsaAI YACGTR 2 cut(s) 695, 2885
BsaHI GRCGYC 1 cut(s) 17
BsaI GGTCTC 1 cut(s) 575
BsaWI WCCGGW 3 cut(s) 2031, 2581, 4131
Bse118I RCCGGY 1 cut(s) 19
Bse1I ACTGG 3 cut(s) 2301, 4401, 4479
Bse3DI GCAATG 4 cut(s) 529, 1270, 2661, 3475
BseAI TCCGGA 3 cut(s) 2031, 2581, 4131
BseBI CCWGG 4 cut(s) 11, 142, 2039, 2510
BseMI GCAATG 4 cut(s) 529, 1270, 2661, 3475
BseNI ACTGG 3 cut(s) 2301, 4401, 4479
BseRI GAGGAG 3 cut(s) 1915, 3807, 4285
BseSI GKGCMC 2 cut(s) 410, 2573
BseX3I CGGCCG 1 cut(s) 50
BseYI CCCAGC 5 cut(s) 1293, 1380, 1574, 3498, 3585
BsgI GTGCAG 2 cut(s) 302, 2585
Bsh1236I CGCG 2 cut(s) 507, 732
Bsh1285I CGRYCG 1 cut(s) 53
BshNI GGYRCC 2 cut(s) 2771, 4112
BsiEI CGRYCG 1 cut(s) 53
BsiHKAI GWGCWC 8 cut(s) 410, 711, 1588, 2573, 2787, 2901, 4200, 4266
BsiHKCI CYCGRG 2 cut(s) 36, 800
BslFI GGGAC 6 cut(s) 783, 2036, 2490, 2973, 3802, 4136
BsmAI GTCTC 1 cut(s) 575
BsmFI GGGAC 6 cut(s) 783, 2036, 2490, 2973, 3802, 4136
BsmI GAATGC 2 cut(s) 3117, 4576
Bso31I GGTCTC 1 cut(s) 575
BsoBI CYCGRG 2 cut(s) 36, 800
Bsp13I TCCGGA 3 cut(s) 2031, 2581, 4131
Bsp143I GATC 9 cut(s) 814, 1254, 1280, 2034, 2137, 3004, 3253, 3485, 4237
BspEI TCCGGA 3 cut(s) 2031, 2581, 4131
BspFNI CGCG 2 cut(s) 507, 732
BspMAI CTGCAG 6 cut(s) 330, 767, 2248, 2705, 2957, 4348
BspMI ACCTGC 5 cut(s) 333, 643, 2237, 2833, 4337
BspPI GGATC 4 cut(s) 1288, 2029, 2042, 3493
BspQI GCTCTTC 1 cut(s) 388
BspT107I GGYRCC 2 cut(s) 2771, 4112
BspTNI GGTCTC 1 cut(s) 575
BsrBI CCGCTC 2 cut(s) 398, 869
BsrDI GCAATG 4 cut(s) 529, 1270, 2661, 3475
BsrFI RCCGGY 1 cut(s) 19
BsrI ACTGG 3 cut(s) 2301, 4401, 4479
BssAI RCCGGY 1 cut(s) 19
BssMI GATC 9 cut(s) 814, 1254, 1280, 2034, 2137, 3004, 3253, 3485, 4237
BssNAI GTATAC 3 cut(s) 1566, 2627, 4232
BssNI GRCGYC 1 cut(s) 17
BssT1I CCWWGG 4 cut(s) 273, 1184, 3389, 4580
Bst1107I GTATAC 3 cut(s) 1566, 2627, 4232
Bst2UI CCWGG 4 cut(s) 11, 142, 2039, 2510
Bst4CI ACNGT 9 cut(s) 111, 1443, 2146, 2158, 2789, 2945, 3648, 4246, 4258
BstACI GRCGYC 1 cut(s) 17
BstAPI GCANNNNNTGC 5 cut(s) 528, 762, 2709, 3123, 3327
BstBAI YACGTR 2 cut(s) 695, 2885
BstC8I GCNNGC 9 cut(s) 21, 502, 1247, 1317, 2418, 2710, 3452, 3522, 4518
BstDSI CCRYGG 4 cut(s) 505, 2201, 2686, 4301
BstEII GGTNACC 1 cut(s) 734
BstENI CCTNNNNNAGG 1 cut(s) 145
BstFNI CGCG 2 cut(s) 507, 732
BstHHI GCGC 1 cut(s) 4592
BstKTI GATC 9 cut(s) 817, 1257, 1283, 2037, 2140, 3007, 3256, 3488, 4240
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 9 cut(s) 814, 1254, 1280, 2034, 2137, 3004, 3253, 3485, 4237
BstMCI CGRYCG 1 cut(s) 53
BstNI CCWGG 4 cut(s) 11, 142, 2039, 2510
BstNSI RCATGY 9 cut(s) 1065, 2078, 2096, 2120, 2420, 3270, 4196, 4220, 4520
BstPAI GACNNNNGTC 4 cut(s) 1635, 2210, 3840, 4310
BstPI GGTNACC 1 cut(s) 734
BstSFI CTRYAG 9 cut(s) 326, 763, 1782, 1887, 2244, 2701, 2953, 3987, 4344
BstSLI GKGCMC 2 cut(s) 410, 2573
BstUI CGCG 2 cut(s) 507, 732
BstV2I GAAGAC 3 cut(s) 909, 1508, 3713
BstX2I RGATCY 1 cut(s) 2034
BstXI CCANNNNNNTGG 2 cut(s) 2222, 4322
BstYI RGATCY 1 cut(s) 2034
BstZ17I GTATAC 3 cut(s) 1566, 2627, 4232
BstZI CGGCCG 1 cut(s) 50
BsuI GTATCC 5 cut(s) 446, 468, 1421, 2627, 2649
BtgI CCRYGG 4 cut(s) 505, 2201, 2686, 4301
BtrI CACGTC 1 cut(s) 4177
BtsI GCAGTG 5 cut(s) 122, 1705, 2524, 2573, 3910
BveI ACCTGC 5 cut(s) 333, 643, 2237, 2833, 4337
Cac8I GCNNGC 9 cut(s) 21, 502, 1247, 1317, 2418, 2710, 3452, 3522, 4518
CaiI CAGNNNCTG 5 cut(s) 1125, 2709, 3021, 3327, 3330
CfoI GCGC 1 cut(s) 4592
Cfr10I RCCGGY 1 cut(s) 19
Cfr42I CCGCGG 1 cut(s) 508
Cfr9I CCCGGG 1 cut(s) 800
CseI GACGC 1 cut(s) 25
Csp6I GTAC 8 cut(s) 308, 350, 595, 751, 2096, 2592, 2772, 2941
CviQI GTAC 8 cut(s) 308, 350, 595, 751, 2096, 2592, 2772, 2941
DpnI GATC 9 cut(s) 816, 1256, 1282, 2036, 2139, 3006, 3255, 3487, 4239
DpnII GATC 9 cut(s) 814, 1254, 1280, 2034, 2137, 3004, 3253, 3485, 4237
DraIII CACNNNGTG 2 cut(s) 414, 2577
EaeI YGGCCR 1 cut(s) 50
EagI CGGCCG 1 cut(s) 50
EciI GGCGGA 2 cut(s) 172, 3605
Ecl136II GAGCTC 3 cut(s) 709, 1586, 2899
EclXI CGGCCG 1 cut(s) 50
Eco130I CCWWGG 4 cut(s) 273, 1184, 3389, 4580
Eco24I GRGCYC 6 cut(s) 271, 340, 711, 1588, 2380, 2901
Eco31I GGTCTC 1 cut(s) 575
Eco47I GGWCC 3 cut(s) 107, 1637, 3842
Eco52I CGGCCG 1 cut(s) 50
Eco53kI GAGCTC 3 cut(s) 709, 1586, 2899
Eco57I CTGAAG 6 cut(s) 921, 1770, 2013, 3975, 4113, 4556
Eco88I CYCGRG 2 cut(s) 36, 800
Eco91I GGTNACC 1 cut(s) 734
EcoICRI GAGCTC 3 cut(s) 709, 1586, 2899
EcoNI CCTNNNNNAGG 1 cut(s) 145
EcoO65I GGTNACC 1 cut(s) 734
EcoRI GAATTC 3 cut(s) 66, 2186, 4286
EcoRII CCWGG 4 cut(s) 9, 140, 2037, 2508
EcoT14I CCWWGG 4 cut(s) 273, 1184, 3389, 4580
EcoT22I ATGCAT 5 cut(s) 974, 2094, 3179, 3475, 4194
EcoT38I GRGCYC 6 cut(s) 271, 340, 711, 1588, 2380, 2901
ErhI CCWWGG 4 cut(s) 273, 1184, 3389, 4580
FalI AAGNNNNNCTT 4 cut(s) 1743, 1775, 3948, 3980
FaqI GGGAC 6 cut(s) 783, 2036, 2490, 2973, 3802, 4136
FauI CCCGC 2 cut(s) 1308, 3513
FauNDI CATATG 1 cut(s) 59
FbaI TGATCA 2 cut(s) 2137, 4237
FblI GTMKAC 3 cut(s) 1565, 2626, 4231
FriOI GRGCYC 6 cut(s) 271, 340, 711, 1588, 2380, 2901
FspBI CTAG 6 cut(s) 164, 318, 1149, 2750, 3354, 4581
GlaI GCGC 1 cut(s) 4591
GsaI CCCAGC 5 cut(s) 1297, 1384, 1578, 3502, 3589
GsuI CTGGAG 6 cut(s) 611, 2075, 2414, 2801, 4175, 4514
HgaI GACGC 1 cut(s) 25
HhaI GCGC 1 cut(s) 4592
Hin1I GRCGYC 1 cut(s) 17
Hin6I GCGC 1 cut(s) 4590
HinP1I GCGC 1 cut(s) 4590
HindIII AAGCTT 3 cut(s) 131, 1700, 3905
Hpy99I CGWCG 3 cut(s) 19, 80, 83
HpyCH4III ACNGT 9 cut(s) 111, 1443, 2146, 2158, 2789, 2945, 3648, 4246, 4258
HpyCH4IV ACGT 4 cut(s) 694, 1513, 2884, 4176
HpySE526I ACGT 4 cut(s) 694, 1513, 2884, 4176
Hsp92I GRCGYC 1 cut(s) 17
HspAI GCGC 1 cut(s) 4590
Kpn2I TCCGGA 3 cut(s) 2031, 2581, 4131
KpnI GGTACC 1 cut(s) 2775
KroI GCCGGC 1 cut(s) 19
KroNI GCCGGC 1 cut(s) 21
Ksp22I TGATCA 2 cut(s) 2137, 4237
KspI CCGCGG 1 cut(s) 508
Kzo9I GATC 9 cut(s) 814, 1254, 1280, 2034, 2137, 3004, 3253, 3485, 4237
LguI GCTCTTC 1 cut(s) 388
MaeI CTAG 6 cut(s) 164, 318, 1149, 2750, 3354, 4581
MaeII ACGT 4 cut(s) 694, 1513, 2884, 4176
MalI GATC 9 cut(s) 816, 1256, 1282, 2036, 2139, 3006, 3255, 3487, 4239
MbiI CCGCTC 2 cut(s) 398, 869
MboI GATC 9 cut(s) 814, 1254, 1280, 2034, 2137, 3004, 3253, 3485, 4237
MflI RGATCY 1 cut(s) 2034
MlyI GAGTC 7 cut(s) 1889, 1994, 2494, 2525, 3784, 4094, 4129
MmeI TCCRAC 6 cut(s) 1171, 1458, 2387, 3376, 3663, 4487
Mph1103I ATGCAT 5 cut(s) 974, 2094, 3179, 3475, 4194
MreI CGCCGGCG 1 cut(s) 19
MroI TCCGGA 3 cut(s) 2031, 2581, 4131
MroNI GCCGGC 1 cut(s) 19
MroXI GAANNNNTTC 2 cut(s) 2349, 4449
Mva1269I GAATGC 2 cut(s) 3117, 4576
MvaI CCWGG 4 cut(s) 11, 142, 2039, 2510
MvnI CGCG 2 cut(s) 507, 732
NaeI GCCGGC 1 cut(s) 21
NciI CCSGG 6 cut(s) 801, 802, 836, 1522, 3026, 3727
NdeI CATATG 1 cut(s) 59
NdeII GATC 9 cut(s) 814, 1254, 1280, 2034, 2137, 3004, 3253, 3485, 4237
NgoMIV GCCGGC 1 cut(s) 19
NmuCI GTSAC 8 cut(s) 695, 734, 1166, 1841, 1946, 2458, 3773, 4046
NsiI ATGCAT 5 cut(s) 974, 2094, 3179, 3475, 4194
NspI RCATGY 9 cut(s) 1065, 2078, 2096, 2120, 2420, 3270, 4196, 4220, 4520
OliI CACNNNNGTG 3 cut(s) 1848, 1953, 4053
PaeI GCATGC 2 cut(s) 2420, 4520
PaqCI CACCTGC 2 cut(s) 643, 2833
PasI CCCWGGG 1 cut(s) 141
PciI ACATGT 2 cut(s) 1061, 3266
PciSI GCTCTTC 1 cut(s) 388
PctI GAATGC 2 cut(s) 3117, 4576
PdiI GCCGGC 1 cut(s) 21
PdmI GAANNNNTTC 2 cut(s) 2349, 4449
PflMI CCANNNNNTGG 2 cut(s) 1190, 3395
PfoI TCCNGGA 1 cut(s) 2037
PleI GAGTC 7 cut(s) 1888, 1993, 2494, 2524, 3784, 4093, 4129
PpsI GAGTC 7 cut(s) 1888, 1993, 2494, 2524, 3784, 4093, 4129
Ppu21I YACGTR 2 cut(s) 695, 2885
PscI ACATGT 2 cut(s) 1061, 3266
PshAI GACNNNNGTC 4 cut(s) 1635, 2210, 3840, 4310
PsiI TTATAA 3 cut(s) 1449, 3602, 3654
Psp124BI GAGCTC 3 cut(s) 711, 1588, 2901
Psp6I CCWGG 4 cut(s) 9, 140, 2037, 2508
PspEI GGTNACC 1 cut(s) 734
PspFI CCCAGC 5 cut(s) 1293, 1380, 1574, 3498, 3585
PspGI CCWGG 4 cut(s) 9, 140, 2037, 2508
PstI CTGCAG 6 cut(s) 330, 767, 2248, 2705, 2957, 4348
PstNI CAGNNNCTG 5 cut(s) 1125, 2709, 3021, 3327, 3330
PsuI RGATCY 1 cut(s) 2034
RsaI GTAC 8 cut(s) 309, 351, 596, 752, 2097, 2593, 2773, 2942
RsaNI GTAC 8 cut(s) 308, 350, 595, 751, 2096, 2592, 2772, 2941
SacI GAGCTC 3 cut(s) 711, 1588, 2901
SacII CCGCGG 1 cut(s) 508
SapI GCTCTTC 1 cut(s) 388
Sau3AI GATC 9 cut(s) 814, 1254, 1280, 2034, 2137, 3004, 3253, 3485, 4237
SbfI CCTGCAGG 2 cut(s) 2248, 4348
ScaI AGTACT 1 cut(s) 596
SchI GAGTC 7 cut(s) 1889, 1994, 2494, 2525, 3784, 4094, 4129
SdaI CCTGCAGG 2 cut(s) 2248, 4348
SfcI CTRYAG 9 cut(s) 326, 763, 1782, 1887, 2244, 2701, 2953, 3987, 4344
Sfr303I CCGCGG 1 cut(s) 508
SgrAI CRCCGGYG 1 cut(s) 19
SgrBI CCGCGG 1 cut(s) 508
SinI GGWCC 3 cut(s) 107, 1637, 3842
SmaI CCCGGG 1 cut(s) 802
SphI GCATGC 2 cut(s) 2420, 4520
Sse8387I CCTGCAGG 2 cut(s) 2248, 4348
SspI AATATT 2 cut(s) 1732, 3937
SspMI CTAG 6 cut(s) 164, 318, 1149, 2750, 3354, 4581
SstI GAGCTC 3 cut(s) 711, 1588, 2901
StyI CCWWGG 4 cut(s) 273, 1184, 3389, 4580
TaaI ACNGT 9 cut(s) 111, 1443, 2146, 2158, 2789, 2945, 3648, 4246, 4258
TaiI ACGT 4 cut(s) 697, 1516, 2887, 4179
TatI WGTACW 4 cut(s) 594, 750, 2095, 2940
TauI GCSGC 4 cut(s) 52, 157, 507, 510
TseFI GTSAC 8 cut(s) 695, 734, 1166, 1841, 1946, 2458, 3773, 4046
Tsp45I GTSAC 8 cut(s) 695, 734, 1166, 1841, 1946, 2458, 3773, 4046
TspGWI ACGGA 8 cut(s) 96, 367, 921, 1452, 1938, 2357, 3657, 4457
TspMI CCCGGG 1 cut(s) 800
Van91I CCANNNNNTGG 2 cut(s) 1190, 3395
VneI GTGCAC 2 cut(s) 406, 2569
VpaK11BI GGWCC 3 cut(s) 107, 1637, 3842
XagI CCTNNNNNAGG 1 cut(s) 145
XapI RAATTY 7 cut(s) 66, 1535, 2084, 2186, 3740, 4184, 4286
XbaI TCTAGA 3 cut(s) 1148, 2749, 3353
XceI RCATGY 9 cut(s) 1065, 2078, 2096, 2120, 2420, 3270, 4196, 4220, 4520
XcmI CCANNNNNNNNNTGG 4 cut(s) 1308, 1421, 3513, 3626
XmaI CCCGGG 1 cut(s) 800
XmaJI CCTAGG 1 cut(s) 4580
XmiI GTMKAC 3 cut(s) 1565, 2626, 4231
XmnI GAANNNNTTC 2 cut(s) 2349, 4449
XspI CTAG 6 cut(s) 164, 318, 1149, 2750, 3354, 4581
ZrmI AGTACT 1 cut(s) 596
Zsp2I ATGCAT 5 cut(s) 974, 2094, 3179, 3475, 4194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.