Prupe.2G123400_v2.0.a1

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000280
Physical Location & Seq
Reverse (-)
17977475 .. 17985273
7799 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G123400.1

Sequence Viewer

Length: 2562 bp
GTAGAACAGAAAGCTGGTGCCGAGTTGAGTGTGATGGGCAGAGGTAGAAAGAGGTCAGGGAAAGACCCAGAAGCGGGTTTTGGAGAAAATGATAGGGGTTTAGAGAAGAAAGAGGGCTTTTTGGAAAATCAAAATGGTGGGACAAAATGGGTAGGTGAAGAAGGTGATGGGGTTGCTTTGAGTTTGAAGAAGAAGAAGCGTGGTGGAGCTTTGGCGGGTGAGAAAGTTGTGGAAGGTGGGGATGGAGCAAAATGGGAAGAGGGTAAAGGCAATTGGGTTGCTCCGACTTGGAAGAAATATAGTAGGCGGAGGAAGAGGGACAGAGATTTGGTGCCTGAGAAAGTTGGGGAAGGTGATAGAGGTCAAGGGCAAGGAAGTACTGGTTTTAGTGGCAAGGAAGTTGAAGGAGCTTCAGCAAAGAAACGTGGTAGACAGAGTAAGAAAATTGAGAAAGTTAAGGCATCTGAAAGGGGAATGGAGAAGCTTGTCAGTGGGAATTGTATTGTTAATAAGAAGAGTGCATATGTTGAGGAGGTGTCTTTGATGTGCCATCAATGTCAGAGGAATGACAAGGCCAGTGTTGTTCGGTGCAGAAGGTGCAAGAGAAAGCGGTTTTGTATCTCTTGCCTAAAAAAATGGTATCCTCAGATGTCAGAGGATGCTATTGCTGAGGCTTGTCCTGTATGCCGTGGAAATTGCAATTGCAAAGCATGCTTACGCTTAGATTTTGATAAAATCTTATGCTGCAAATTCTGCATTAGCGAAGATAAAAAAGTTGAGCACTCAAAGTATTTGCTCCAAGTACTACTTCCATTCTTGAAAAGAGTTAATGATGAACAAATGATTGAGATGGAGATCGAGGCTAGGAGACAAGGGCTATCTCTTTCGGAGCTAAAGATACAAAGATCAGATTCCTCCTATGGTGATCGAGTGTACTGCAACAACTGCAAAACTTCTATTATTGATTTACACAGAAGCTGTCCTATATGTTCATATGATCTTTGCCTCATCTGTTGCCGAGAGATTCGAGATGGACACCTGCTGGGAGAACTGCCTTCAGAAACTAGCCACACTTGTCGTACAGGGTCAACATCCGAGAGGAAAGCAAATTTAGATGGAAGTATTTTTTGCCCCCCAGAGAACAAGGATGGCTGCCGTCAAAGTTTTCTAGAATTGAGATGCATGTTTTCTGAAAATGATGTCAGAGAGTTGGTTAAGAAAGCAGAGGAAATTGCTCAAACTTACAAATTTACGCACGCGGCTGGAATTTCTGCACAACAGTGTTCATGTTCTAATTCTGCAGGTGTTCTTGACTTGAGCAGCAATAAGTTAAGGAAAGCAGCTTCTCGAGAGGGTTCTGATGACAACTTCTTGTACTGTCCAAGGGCTGGAGATATCCAACATGAGGAGTTTAAGCATTTTCAATGTCATTGGATCAGAGGTGAGCCTGTGATTGTGAGCAATGTGCTTGAAACTGCATCAGGTCTAAGCTGGGAACCAACGGTCATGTGGCGTGCTTGTCGTGAGATGAAGCGTACTAGGCATAAGAAACATTTGGAAGTCAAGGCCATTGATTGCTTAGATTGGTGTGAGCTAGATATCGGTATCCACAAGTTTTTTACTGGATATTCAAAAGGCCGTTTTGATCGGCAAATGTGGCCGCAGATGCTAAAACTCAACGATCCGTCCCTGGATGACTTTTTCAAAGAGCGCCTTCCTCGTCACTGTGCTGAGTTTATTTGCTACTTGCCGTTTGAGGAATATACTCATCCTCACAGAGGCTTTTTGAACCTTGCTCCAGATATGGGGCCAAAGACATATATTGCTTATGGTGTTTCCGAGGAGCTTGGACGTGGAGATTCTGTAACCAAGCTTCACTGCAATTCCTGTGATGTGGTGAATATTCTGACACATACTGCTGAATTTACCCTTACTCCCAAGGACATTGAAAATATGAAAAGGTTGAAAAAAAAGCACATTGAACAAGACAGAAGGGAAATTAACAAGGCTGGCGGTGGCAGGGCTGCAAATGATAAGAAATTTTTTTTTGAAGTTGACAATCAAAACAAGGGTGCAGCTTTTCATGAGCTGACTGATCCAGCTGTTCAACCTGATGGTGATTCTTGTGTTTCTAGTTTGAATGCTGGAAGTTTTAAAGAGGGATCCAAGTCTGAAAAGAATCGAGAAGATGTTCCTAAGCTGCAGGATTATCTCCGGAAGCACTACAAGGAATTCAGGCATACCTACTGTTGTCCGCTGCCACAGGTTATTCATCCCATACATGATCAGACCTTTTATCTGACTCTGGAGCACAAAAGGAAGCTCAAGGAAGAATATGGAATTGAACCATGGACATTTATCCAAAACCTTGGGGATGCTGTTTTAATTCCGGCAGGATGTCCACACCAAGTGAGAAACTTGAAGTCGTGCATTAAAGTCGCACTTGAATTTGTCTCTCCTGAAAATGTTGGGGAGTGCATACGGTTGACAGATGAATTCCGCACACTTCCACAAAATCATAGGGCTAAAGAGGACAAATTGGAGGTACGCCGTCTTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

854

Amino Acids

97.02

Weight (kDa)

8.7

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1047, 1292
Acc36I ACCTGC 2 cut(s) 1047, 1292
AccB1I GGYRCC 2 cut(s) 17, 331
AccB7I CCANNNNNTGG 1 cut(s) 1388
AccI GTMKAC 1 cut(s) 430
AccII CGCG 1 cut(s) 1259
AccIII TCCGGA 1 cut(s) 2214
AciI CCGC 9 cut(s) 74, 215, 307, 610, 1259, 1661, 2013, 2255, 2500
AclWI GGATC 5 cut(s) 1442, 1676, 2090, 2157, 2170
AcoI YGGCCR 1 cut(s) 1658
AcsI RAATTY 9 cut(s) 749, 1108, 1247, 1266, 1922, 2039, 2231, 2447, 2495
AcuI CTGAAG 2 cut(s) 396, 1041
AdeI CACNNNGTG 1 cut(s) 2410
AfaI GTAC 7 cut(s) 379, 804, 935, 1081, 1376, 1537, 2547
AfiI CCNNNNNNNGG 6 cut(s) 73, 74, 1388, 1405, 1778, 1805
AjiI CACGTC 1 cut(s) 1853
AjnI CCWGG 1 cut(s) 1689
AjuI GAANNNNNNNTTGG 4 cut(s) 63, 95, 792, 824
AleI CACNNNNGTG 1 cut(s) 1279
Alw21I GWGCWC 2 cut(s) 783, 2313
Alw26I GTCTC 2 cut(s) 862, 2458
AlwI GGATC 5 cut(s) 1442, 1676, 2090, 2157, 2170
AlwNI CAGNNNCTG 1 cut(s) 978
Ama87I CYCGRG 1 cut(s) 1347
Aor13HI TCCGGA 1 cut(s) 2214
AoxI GGCC 5 cut(s) 573, 1566, 1636, 1658, 1808
ApeKI GCWGC 8 cut(s) 744, 1152, 1320, 1340, 2024, 2075, 2200, 2257
ApoI RAATTY 9 cut(s) 749, 1108, 1247, 1266, 1922, 2039, 2231, 2447, 2495
Asp700I GAANNNNTTC 1 cut(s) 2190
AspLEI GCGC 1 cut(s) 1713
AspS9I GGNCC 1 cut(s) 1808
AsuHPI GGTGA 8 cut(s) 167, 176, 230, 365, 935, 1454, 1909, 2129
AvaI CYCGRG 1 cut(s) 1347
BamHI GGATCC 1 cut(s) 2162
BanI GGYRCC 2 cut(s) 17, 331
BarI GAAGNNNNNNTAC 3 cut(s) 1857, 1889, 2538
BbsI GAAGAC 1 cut(s) 2546
Bbv12I GWGCWC 2 cut(s) 783, 2313
BbvCI CCTCAGC 1 cut(s) 669
BbvI GCAGC 8 cut(s) 731, 1139, 1332, 1352, 2011, 2087, 2187, 2244
BccI CCATC 9 cut(s) 28, 161, 236, 558, 844, 1025, 1109, 1142, 2108
BceAI ACGGC 5 cut(s) 672, 1140, 1623, 1735, 2535
BcgI CGANNNNNNTGC 4 cut(s) 918, 952, 2419, 2453
BciT130I CCWGG 1 cut(s) 1691
BciVI GTATCC 2 cut(s) 651, 1616
BclI TGATCA 1 cut(s) 2284
BcoDI GTCTC 2 cut(s) 862, 2458
BfaI CTAG 6 cut(s) 864, 1065, 1169, 1539, 1595, 2133
BfmI CTRYAG 3 cut(s) 1299, 2201, 2558
BfoI RGCGCY 1 cut(s) 1714
BfuAI ACCTGC 2 cut(s) 1047, 1292
BfuI GTATCC 2 cut(s) 651, 1616
BmcAI AGTACT 2 cut(s) 379, 804
Bme1390I CCNGG 1 cut(s) 1691
BmeT110I CYCGRG 1 cut(s) 1347
BmgBI CACGTC 1 cut(s) 1853
BmgT120I GGNCC 1 cut(s) 1808
BmiI GGNNCC 5 cut(s) 19, 333, 1497, 1809, 2164
BmrFI CCNGG 1 cut(s) 1691
BmsI GCATC 6 cut(s) 470, 649, 1169, 1487, 1656, 2365
BpiI GAAGAC 1 cut(s) 2546
BpmI CTGGAG 3 cut(s) 1410, 1782, 2327
Bpu10I CCTNAGC 2 cut(s) 669, 2196
BpuEI CTTGAG 2 cut(s) 1336, 2309
BsaBI GATNNNNATC 1 cut(s) 854
BsaJI CCNNGG 7 cut(s) 688, 1382, 1689, 1839, 1938, 2348, 2368
BsaWI WCCGGW 1 cut(s) 2214
BsaXI ACNNNNNCTCC 4 cut(s) 1848, 1878, 1918, 1948
Bsc4I CCNNNNNNNGG 6 cut(s) 73, 74, 1388, 1405, 1778, 1805
Bse1I ACTGG 3 cut(s) 385, 576, 1627
Bse3DI GCAATG 1 cut(s) 1468
Bse8I GATNNNNATC 1 cut(s) 854
BseAI TCCGGA 1 cut(s) 2214
BseBI CCWGG 1 cut(s) 1691
BseDI CCNNGG 7 cut(s) 688, 1382, 1689, 1839, 1938, 2348, 2368
BseGI GGATG 9 cut(s) 247, 664, 1091, 1153, 1699, 1768, 2272, 2380, 2402
BseJI GATNNNNATC 1 cut(s) 854
BseLI CCNNNNNNNGG 6 cut(s) 73, 74, 1388, 1405, 1778, 1805
BseMI GCAATG 1 cut(s) 1468
BseMII CTCAG 4 cut(s) 327, 659, 660, 1722
BseNI ACTGG 3 cut(s) 385, 576, 1627
BseRI GAGGAG 3 cut(s) 545, 1421, 1856
BseXI GCAGC 8 cut(s) 731, 1139, 1332, 1352, 2011, 2087, 2187, 2244
BseYI CCCAGC 2 cut(s) 1042, 1491
BsgI GTGCAG 3 cut(s) 610, 1257, 2094
Bsh1236I CGCG 1 cut(s) 1259
BshFI GGCC 5 cut(s) 575, 1568, 1638, 1660, 1810
BshNI GGYRCC 2 cut(s) 17, 331
BsiHKAI GWGCWC 2 cut(s) 783, 2313
BsiHKCI CYCGRG 1 cut(s) 1347
BsiSI CCGG 2 cut(s) 2215, 2390
BslFI GGGAC 3 cut(s) 154, 332, 1672
BslI CCNNNNNNNGG 6 cut(s) 73, 74, 1388, 1405, 1778, 1805
BsmAI GTCTC 2 cut(s) 862, 2458
BsmFI GGGAC 3 cut(s) 154, 332, 1672
BsmI GAATGC 1 cut(s) 2146
BsnI GGCC 5 cut(s) 575, 1568, 1638, 1660, 1810
BsoBI CYCGRG 1 cut(s) 1347
Bsp1286I GDGCHC 2 cut(s) 783, 2313
Bsp13I TCCGGA 1 cut(s) 2214
Bsp19I CCATGG 1 cut(s) 2348
BspACI CCGC 9 cut(s) 74, 215, 307, 610, 1259, 1661, 2013, 2255, 2500
BspANI GGCC 5 cut(s) 575, 1568, 1638, 1660, 1810
BspCNI CTCAG 4 cut(s) 328, 658, 661, 1723
BspEI TCCGGA 1 cut(s) 2214
BspFNI CGCG 1 cut(s) 1259
BspHI TCATGA 1 cut(s) 2083
BspLI GGNNCC 5 cut(s) 19, 333, 1497, 1809, 2164
BspMAI CTGCAG 2 cut(s) 1303, 2205
BspMI ACCTGC 2 cut(s) 1047, 1292
BspPI GGATC 5 cut(s) 1442, 1676, 2090, 2157, 2170
BspT107I GGYRCC 2 cut(s) 17, 331
BsrDI GCAATG 1 cut(s) 1468
BsrI ACTGG 3 cut(s) 385, 576, 1627
BssECI CCNNGG 7 cut(s) 688, 1382, 1689, 1839, 1938, 2348, 2368
BssT1I CCWWGG 4 cut(s) 1382, 1938, 2348, 2368
Bst2UI CCWGG 1 cut(s) 1691
Bst4CI ACNGT 6 cut(s) 1281, 1379, 1504, 1727, 2249, 2484
Bst6I CTCTTC 3 cut(s) 252, 308, 509
BstAPI GCANNNNNTGC 4 cut(s) 597, 711, 753, 945
BstC8I GCNNGC 4 cut(s) 712, 1257, 1515, 2011
BstDEI CTNAG 8 cut(s) 336, 645, 669, 721, 1487, 1579, 1731, 2196
BstDSI CCRYGG 2 cut(s) 688, 2348
BstENI CCTNNNNNAGG 1 cut(s) 1776
BstF5I GGATG 9 cut(s) 247, 664, 1091, 1153, 1699, 1768, 2272, 2380, 2402
BstFNI CGCG 1 cut(s) 1259
BstH2I RGCGCY 1 cut(s) 1714
BstHHI GCGC 1 cut(s) 1713
BstMAI GTCTC 2 cut(s) 862, 2458
BstMWI GCNNNNNNNGC 7 cut(s) 597, 711, 753, 945, 1540, 1657, 1666
BstNI CCWGG 1 cut(s) 1691
BstNSI RCATGY 2 cut(s) 714, 1186
BstSCI CCNGG 1 cut(s) 1689
BstSFI CTRYAG 3 cut(s) 1299, 2201, 2558
BstUI CGCG 1 cut(s) 1259
BstV1I GCAGC 8 cut(s) 731, 1139, 1332, 1352, 2011, 2087, 2187, 2244
BstV2I GAAGAC 1 cut(s) 2546
BstX2I RGATCY 1 cut(s) 2162
BstXI CCANNNNNNTGG 1 cut(s) 2369
BstYI RGATCY 1 cut(s) 2162
BsuI GTATCC 2 cut(s) 651, 1616
BsuRI GGCC 5 cut(s) 575, 1568, 1638, 1660, 1810
BtgI CCRYGG 2 cut(s) 688, 2348
BtrI CACGTC 1 cut(s) 1853
BtsCI GGATG 9 cut(s) 247, 664, 1091, 1153, 1699, 1768, 2272, 2380, 2402
BtsI GCAGTG 1 cut(s) 1876
BtsIMutI CAGTG 5 cut(s) 496, 583, 1286, 1723, 1876
BveI ACCTGC 2 cut(s) 1047, 1292
Cac8I GCNNGC 4 cut(s) 712, 1257, 1515, 2011
CaiI CAGNNNCTG 1 cut(s) 978
CciI TCATGA 1 cut(s) 2083
CfoI GCGC 1 cut(s) 1713
Cfr13I GGNCC 1 cut(s) 1808
Csp6I GTAC 7 cut(s) 378, 803, 934, 1080, 1375, 1536, 2546
CviAII CATG 8 cut(s) 711, 1183, 1287, 1403, 1507, 2084, 2282, 2349
CviQI GTAC 7 cut(s) 378, 803, 934, 1080, 1375, 1536, 2546
DdeI CTNAG 8 cut(s) 336, 645, 669, 721, 1487, 1579, 1731, 2196
DraI TTTAAA 1 cut(s) 2155
DraIII CACNNNGTG 1 cut(s) 2410
EaeI YGGCCR 1 cut(s) 1658
Eam1104I CTCTTC 3 cut(s) 252, 308, 509
EarI CTCTTC 3 cut(s) 252, 308, 509
EciI GGCGGA 1 cut(s) 322
Eco130I CCWWGG 4 cut(s) 1382, 1938, 2348, 2368
Eco32I GATATC 2 cut(s) 1396, 1600
Eco57I CTGAAG 2 cut(s) 396, 1041
Eco88I CYCGRG 1 cut(s) 1347
EcoNI CCTNNNNNAGG 1 cut(s) 1776
EcoRI GAATTC 2 cut(s) 2231, 2495
EcoRII CCWGG 1 cut(s) 1689
EcoRV GATATC 2 cut(s) 1396, 1600
EcoT14I CCWWGG 4 cut(s) 1382, 1938, 2348, 2368
EcoT22I ATGCAT 1 cut(s) 1184
ErhI CCWWGG 4 cut(s) 1382, 1938, 2348, 2368
FaeI CATG 8 cut(s) 714, 1186, 1290, 1406, 1510, 2087, 2285, 2352
FalI AAGNNNNNCTT 6 cut(s) 792, 824, 1698, 1730, 2427, 2459
FaqI GGGAC 3 cut(s) 154, 332, 1672
FatI CATG 8 cut(s) 710, 1182, 1286, 1402, 1506, 2083, 2281, 2348
FauI CCCGC 2 cut(s) 67, 208
FauNDI CATATG 2 cut(s) 523, 994
FbaI TGATCA 1 cut(s) 2284
FblI GTMKAC 1 cut(s) 430
FokI GGATG 9 cut(s) 254, 671, 1078, 1160, 1706, 1755, 2259, 2387, 2409
FspBI CTAG 6 cut(s) 864, 1065, 1169, 1539, 1595, 2133
GlaI GCGC 1 cut(s) 1712
GsaI CCCAGC 2 cut(s) 1046, 1495
GsuI CTGGAG 3 cut(s) 1410, 1782, 2327
HaeII RGCGCY 1 cut(s) 1714
HaeIII GGCC 5 cut(s) 575, 1568, 1638, 1660, 1810
HapII CCGG 2 cut(s) 2215, 2390
HhaI GCGC 1 cut(s) 1713
Hin1II CATG 8 cut(s) 714, 1186, 1290, 1406, 1510, 2087, 2285, 2352
Hin6I GCGC 1 cut(s) 1711
HinP1I GCGC 1 cut(s) 1711
HincII GTYRAC 3 cut(s) 1089, 2056, 2487
HindII GTYRAC 3 cut(s) 1089, 2056, 2487
HindIII AAGCTT 2 cut(s) 482, 1871
HinfI GANTC 6 cut(s) 911, 1024, 1859, 2120, 2179, 2302
HpaII CCGG 2 cut(s) 2215, 2390
HphI GGTGA 8 cut(s) 167, 176, 230, 365, 935, 1454, 1909, 2129
Hpy166II GTNNAC 6 cut(s) 431, 934, 1089, 2056, 2402, 2487
Hpy8I GTNNAC 6 cut(s) 431, 934, 1089, 2056, 2402, 2487
HpyAV CCTTC 8 cut(s) 155, 227, 344, 398, 588, 1065, 1724, 1986
HpyCH4III ACNGT 6 cut(s) 1281, 1379, 1504, 1727, 2249, 2484
HpyCH4IV ACGT 2 cut(s) 424, 1852
HpyF10VI GCNNNNNNNGC 7 cut(s) 597, 711, 753, 945, 1540, 1657, 1666
HpyF3I CTNAG 8 cut(s) 336, 645, 669, 721, 1487, 1579, 1731, 2196
HpySE526I ACGT 2 cut(s) 424, 1852
Hsp92II CATG 8 cut(s) 714, 1186, 1290, 1406, 1510, 2087, 2285, 2352
HspAI GCGC 1 cut(s) 1711
Kpn2I TCCGGA 1 cut(s) 2214
Ksp22I TGATCA 1 cut(s) 2284
LmnI GCTCC 9 cut(s) 206, 245, 286, 407, 801, 889, 1801, 1843, 2308
Lsp1109I GCAGC 8 cut(s) 731, 1139, 1332, 1352, 2011, 2087, 2187, 2244
LweI GCATC 6 cut(s) 470, 649, 1169, 1487, 1656, 2365
MaeI CTAG 6 cut(s) 864, 1065, 1169, 1539, 1595, 2133
MaeII ACGT 2 cut(s) 424, 1852
MaeIII GTNAC 2 cut(s) 1721, 1864
MfeI CAATTG 2 cut(s) 271, 700
MflI RGATCY 1 cut(s) 2162
MhlI GDGCHC 2 cut(s) 783, 2313
MlyI GAGTC 1 cut(s) 2296
MmeI TCCRAC 2 cut(s) 308, 1423
Mph1103I ATGCAT 1 cut(s) 1184
MroI TCCGGA 1 cut(s) 2214
MroXI GAANNNNTTC 1 cut(s) 2190
MslI CAYNNNNRTG 1 cut(s) 1279
MspA1I CMGCKG 2 cut(s) 2102, 2257
MspI CCGG 2 cut(s) 2215, 2390
MspR9I CCNGG 1 cut(s) 1691
MunI CAATTG 2 cut(s) 271, 700
Mva1269I GAATGC 1 cut(s) 2146
MvaI CCWGG 1 cut(s) 1691
MvnI CGCG 1 cut(s) 1259
MwoI GCNNNNNNNGC 7 cut(s) 597, 711, 753, 945, 1540, 1657, 1666
NcoI CCATGG 1 cut(s) 2348
NdeI CATATG 2 cut(s) 523, 994
NlaIII CATG 8 cut(s) 714, 1186, 1290, 1406, 1510, 2087, 2285, 2352
NlaIV GGNNCC 5 cut(s) 19, 333, 1497, 1809, 2164
NmeAIII GCCGAG 2 cut(s) 46, 1043
NmuCI GTSAC 1 cut(s) 1721
NsiI ATGCAT 1 cut(s) 1184
NspI RCATGY 2 cut(s) 714, 1186
OliI CACNNNNGTG 1 cut(s) 1279
PaeI GCATGC 1 cut(s) 714
PaeR7I CTCGAG 1 cut(s) 1347
PagI TCATGA 1 cut(s) 2083
PaqCI CACCTGC 2 cut(s) 1047, 1292
PctI GAATGC 1 cut(s) 2146
PdmI GAANNNNTTC 1 cut(s) 2190
PfeI GAWTC 5 cut(s) 911, 1024, 1859, 2120, 2179
PflMI CCANNNNNTGG 1 cut(s) 1388
PleI GAGTC 1 cut(s) 2296
PpsI GAGTC 1 cut(s) 2296
Psp6I CCWGG 1 cut(s) 1689
PspFI CCCAGC 2 cut(s) 1042, 1491
PspGI CCWGG 1 cut(s) 1689
PspN4I GGNNCC 5 cut(s) 19, 333, 1497, 1809, 2164
PspPI GGNCC 1 cut(s) 1808
PstI CTGCAG 2 cut(s) 1303, 2205
PstNI CAGNNNCTG 1 cut(s) 978
PsuI RGATCY 1 cut(s) 2162
PvuII CAGCTG 1 cut(s) 2102
RsaI GTAC 7 cut(s) 379, 804, 935, 1081, 1376, 1537, 2547
RsaNI GTAC 7 cut(s) 378, 803, 934, 1080, 1375, 1536, 2546
RseI CAYNNNNRTG 1 cut(s) 1279
Sau96I GGNCC 1 cut(s) 1808
ScaI AGTACT 2 cut(s) 379, 804
SchI GAGTC 1 cut(s) 2296
ScrFI CCNGG 1 cut(s) 1691
SduI GDGCHC 2 cut(s) 783, 2313
SfaNI GCATC 6 cut(s) 470, 649, 1169, 1487, 1656, 2365
SfcI CTRYAG 3 cut(s) 1299, 2201, 2558
Sfr274I CTCGAG 1 cut(s) 1347
SlaI CTCGAG 1 cut(s) 1347
SmiMI CAYNNNNRTG 1 cut(s) 1279
SmlI CTYRAG 3 cut(s) 1315, 1347, 2324
SmoI CTYRAG 3 cut(s) 1315, 1347, 2324
SphI GCATGC 1 cut(s) 714
SsiI CCGC 9 cut(s) 74, 215, 307, 610, 1259, 1661, 2013, 2255, 2500
SspI AATATT 1 cut(s) 1903
SspMI CTAG 6 cut(s) 864, 1065, 1169, 1539, 1595, 2133
StyD4I CCNGG 1 cut(s) 1689
StyI CCWWGG 4 cut(s) 1382, 1938, 2348, 2368
TaaI ACNGT 6 cut(s) 1281, 1379, 1504, 1727, 2249, 2484
TaiI ACGT 2 cut(s) 427, 1855
TaqI TCGA 5 cut(s) 858, 928, 1027, 1348, 2182
TatI WGTACW 4 cut(s) 377, 802, 933, 1374
TauI GCSGC 2 cut(s) 1262, 1663
TfiI GAWTC 5 cut(s) 911, 1024, 1859, 2120, 2179
TscAI CASTG 5 cut(s) 496, 583, 1286, 1730, 1883
TseFI GTSAC 1 cut(s) 1721
TseI GCWGC 8 cut(s) 744, 1152, 1320, 1340, 2024, 2075, 2200, 2257
Tsp45I GTSAC 1 cut(s) 1721
TspDTI ATGAA 8 cut(s) 849, 981, 1275, 1544, 1970, 2072, 2261, 2508
TspGWI ACGGA 1 cut(s) 1674
TspRI CASTG 5 cut(s) 496, 583, 1286, 1730, 1883
Van91I CCANNNNNTGG 1 cut(s) 1388
XagI CCTNNNNNAGG 1 cut(s) 1776
XapI RAATTY 9 cut(s) 749, 1108, 1247, 1266, 1922, 2039, 2231, 2447, 2495
XbaI TCTAGA 1 cut(s) 1168
XceI RCATGY 2 cut(s) 714, 1186
XcmI CCANNNNNNNNNTGG 1 cut(s) 1506
XhoI CTCGAG 1 cut(s) 1347
XmiI GTMKAC 1 cut(s) 430
XmnI GAANNNNTTC 1 cut(s) 2190
XspI CTAG 6 cut(s) 864, 1065, 1169, 1539, 1595, 2133
ZrmI AGTACT 2 cut(s) 379, 804
Zsp2I ATGCAT 1 cut(s) 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.