RchiOBHm_Chr7g0215021

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000280
Physical Location & Seq
Forward (+)
32450040 .. 32460171
10132 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19239

Sequence Viewer

Length: 3021 bp
ATGGCGAGAGGGGGAGGCAGAGGAAGAGGGAGATGGGCGAAGCGGCAAACCCATAACCCAAATCAAACTGAAGATGTTGCTGTCAAAGTTGGTGAAGATGGTGATGGGGTTGTTCTTTCTGAGATAAAGGCTGTGTCTTTTGGTGGTGTAGAGAAGGGTGAGGACGGTTTGGGAAACGATCAAAAGGAAGAAGTTGCTGTGAGTGTGAAGAAGAAGAAGAAGCCTGGGAGGAAGAGTAAGAAGGGTTTGGTGGGAAATGGTGATGGGGGTCTAAAGGAGGTTGCTTTTGGTGGTGATGAGATTTCTATGATGACTTTGAGGAAGAGGCCTGAACGGAACAAGAGAGTTAGAGTTATGGAGGCAGAGGGAAATGGTGAGAAAGGTAAAGAGAATGGTGCTTCTGTGAACAAGAAGGGTAGGAAAAAGGTGACATTTGCGAAGGAAGGGGGAAAGAAAGGTGTTTTAGAAGTGGATAATGACAAGAAGCGCGATAGGAAGCGTGCGAAAACTGAGGAGGAAGATGGGAATGAGGTAGGCTATTCGTTTAGGCCTCTGAGGGAGCAGGGACATCTATCAACCGAGGAGCGTAAAAAGTGGATTGAGGAGGTATCCCTAATGTGCCACCAATGTCAGAGGAATGATAAGGGAAGGGTTGTTCGGTGCAAAAAGTGCAGGACAAAGCGATATTGTGTTCCTTGCATAGAGAATTGGTATCCACATACATCAGAGGATGCCATTGCTGAGGCTTGTCCTGTATGCCGTGGAAATTGCAATTGCAAAGCATGCTTGCGCTTAGATATACCAGTTAAAAATCTGAAGAACCAAGATTTAGTACTTGACAAAGATGCAAGAATTGAGCACTCTAAGTACTTAATTCATGCACTGCTTCCATCCTTGAAAAGAATTCATGAAGAACAAGAGAATGAGATGGCGATGGAGGCAAGGAGAAAAGGATTATCTAACTTGAAGCTCCAGATACCAAAGTCTGACTGTGCTGCTGATGTTCGCGTGTATTGCAACAACTGCAGAACTTCTATTTTTGATTATCACAGAAGCTGTCCTAAATGTTCATATGACCTCTGCCTCATTTGTTGCCGGGAGATTCGAGAAGGACAATTGCAGCTAGGTAGGGAGGAAGTGACTTTGGAGTATGTTTCTCGGGGCTTACCTTATTTGCATGGTGGAAAGGAAGAGGACAACAAAAAAAGAAAGGGAAAGAGAAATAGGAATGTTGTGGAGCCACCCTCTGAAACTAGTCCCAAAAGTTCTGGAAAGCGAACATTTGAGTGGAAATCATATGAAGATGGAAGCATAATTTGCCCCCCAGAGGACATGGGAGGTTGTGGCAGTGGTAATCTAGAATTGAGATGCATGTTTTCTAAAAATTACGTCAAAGAGTTGGTAAAGAAAGCAGAAGAGATAGATGAAGCTTACAAACTTGTGTATTCAAGTAGAACTTCTGCAGAGAGGTGTTCATGTCTCAACTCTGTCGATGATGTCAATTCGAGCATCAATGCATCAAGGAAAGCAGCTTCACGAGACAATTCTGAGGACAACTATCTGTACTGTGCAAGGGCTGGAGATATCCAAGTAGAGGATTTAAAGCATTTCCAGTGGCATTGGATCAAGGGTGAGCCTGTTATCGTTAGCAATGCACTTGAAACTGCATCAGGTTTAAGCTGGGAACCATTGGTCATGTGGCGGGCTTGTCGTCAGATGCAACATACTAAGCACGGTAAACATTTGGACGTCAAAGCAATAGATTGCCTGGATTGGTGTGAGGGGGATATCAATATCCACCAATTTTTTACTGGATACTCAAAGGGACGGTTTGATAAAGAGAATTGGCCACAGATATTGAAACTGAAAGATTGGCCGCCAACTAATTTATTTGAGGAGCGCCTTCCTCGTCATGGTGCTGAGTTCATTTGTTGTCTGCCGTATAAGGAATATACTCATCCTCGCAATGGTCCTCTAAACCTTTTTGCCAAATTACCTTCAAAATGTGTAAAACCAGACATGGGACCAAAGACATATATTGCTTATGGTTTTTCCCAGGAGCTTGGACGTGGAGATTCTGTAACCAAGCTTCATTGTGATATGTCGGATGCGGTGAATGTTTTGACACACACCACTGAAGTGAACCTTACTCCTGAGCAACTTACTGTCATTGAAAAGTTGAAAAAGAAGCATGCTCAACAAGACCAAAGAGAAATTTTTGGCAATTGTCAGACTGTGGATGATGATGTTGATAGTGGCAAGGCTGCTGGTGATTCTTGTTTCAGTAATGATAAACAACTTCTTAATGAAGTTGATAATAATAGCAGTGGTGTAGCTGTTCCAGAACTGGTTGATCCAATTGTTCAACATGTTGGAGATGCTTCAACGGGATCTGTGTCCGAAAAGAATAAAGAGGCAGAATTAGATCATGATATCGGTGGTGAAATGCCTGCAAGAATTTATGGAAATAAGTTGGAAGAGTCGGATGTCAATGAGGGAGGTGCTTTATGGGACATATTCCGTAGACAGGATGTTCCTAAGCTGCAGGAGTATCTCATGAAGCATTTCAAGGAATTCAGGCACATACACTGTTGTCCATTGCAGCAGGTTATTCATCCCATACATGATCAGACATTTTATCTTACAATGGAGCACAAAAAGAAGCTTAAAGAGGAATATGGAATTGAACCATGGACCTTCATTCAAAACCTTGGAGATGCTGTCTTTATTCCTGCAGGATGTCCGCATCAAGTCAGGAACTTGAAGTCATGCATCAAAGTTGCAATGGACTTTGTCTCACCTGAAAATGTCGGCGAGTGCTTTCACTTGACAGAAGTATTCCGCACACTTCCAGAAGATCATAGGGCTAAAGAGGACAAATTGGAGGTAAAGAAAATGATTGTTCATTCTGTCACTGATGCTCTGGAGGAACTAAATCAGAATGTGAGGTCTAAGGAAGTCACTAATCCACCTCAATCAAGTCAGAGTGATGGAGATTATATGGTCCAGGATATTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1006

Amino Acids

113.92

Weight (kDa)

7.61

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 204 - 264 5.1e-07 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 834 - 931 7.7e-15 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1752
Acc36I ACCTGC 1 cut(s) 2596
AccI GTMKAC 1 cut(s) 2524
AccII CGCG 2 cut(s) 489, 1008
AciI CCGC 6 cut(s) 43, 1702, 1877, 2111, 2744, 2842
AclWI GGATC 3 cut(s) 1631, 2348, 2398
AcoI YGGCCR 2 cut(s) 1847, 1874
AcsI RAATTY 4 cut(s) 903, 2214, 2457, 2573
AcuI CTGAAG 3 cut(s) 90, 836, 2157
AcyI GRCGYC 1 cut(s) 1749
AfaI GTAC 3 cut(s) 834, 869, 1565
AfiI CCNNNNNNNGG 6 cut(s) 1327, 1594, 1913, 1967, 2021, 2527
AflIII ACRYGT 1 cut(s) 2368
AhlI ACTAGT 1 cut(s) 1253
AjiI CACGTC 1 cut(s) 2069
AjnI CCWGG 4 cut(s) 223, 1767, 2055, 3006
AleI CACNNNNGTG 1 cut(s) 2138
AloI GAACNNNNNNTCC 2 cut(s) 639, 671
Alw21I GWGCWC 2 cut(s) 861, 2655
Alw26I GTCTC 3 cut(s) 1484, 1533, 2800
AlwI GGATC 3 cut(s) 1631, 2348, 2398
AlwNI CAGNNNCTG 1 cut(s) 1056
Ama87I CYCGRG 1 cut(s) 1158
AoxI GGCC 4 cut(s) 326, 548, 1847, 1874
ApeKI GCWGC 6 cut(s) 995, 1120, 1529, 2264, 2542, 2602
ApoI RAATTY 4 cut(s) 903, 2214, 2457, 2573
Asp700I GAANNNNTTC 1 cut(s) 2564
AspLEI GCGC 3 cut(s) 489, 792, 1902
AspS9I GGNCC 4 cut(s) 1970, 2024, 2694, 3004
AsuC2I CCSGG 1 cut(s) 1097
AvaI CYCGRG 1 cut(s) 1158
AvaII GGWCC 4 cut(s) 1970, 2024, 2694, 3004
BaeI ACNNNNGTAYC 2 cut(s) 968, 1001
BalI TGGCCA 1 cut(s) 1849
BarI GAAGNNNNNNTAC 2 cut(s) 2073, 2105
BauI CACGAG 1 cut(s) 1536
Bbv12I GWGCWC 2 cut(s) 861, 2655
BbvCI CCTCAGC 1 cut(s) 741
BbvI GCAGC 6 cut(s) 982, 1132, 1541, 2251, 2529, 2614
BceAI ACGGC 2 cut(s) 744, 1924
BciT130I CCWGG 4 cut(s) 225, 1769, 2057, 3008
BciVI GTATCC 3 cut(s) 619, 723, 1808
BclI TGATCA 1 cut(s) 2626
BcnI CCSGG 1 cut(s) 1097
BcoDI GTCTC 3 cut(s) 1484, 1533, 2800
BcuI ACTAGT 1 cut(s) 1253
BfaI CTAG 3 cut(s) 1124, 1254, 1358
BfmI CTRYAG 4 cut(s) 1024, 1461, 2543, 2733
BfoI RGCGCY 1 cut(s) 1903
BfuAI ACCTGC 1 cut(s) 2596
BfuI GTATCC 3 cut(s) 619, 723, 1808
BisI GCNGC 8 cut(s) 44, 996, 1121, 1530, 1877, 2265, 2543, 2603
BlsI GCNGC 8 cut(s) 45, 997, 1122, 1531, 1878, 2266, 2544, 2604
BmcAI AGTACT 2 cut(s) 834, 869
Bme1390I CCNGG 5 cut(s) 225, 1097, 1769, 2057, 3008
Bme18I GGWCC 4 cut(s) 1970, 2024, 2694, 3004
BmeT110I CYCGRG 1 cut(s) 1158
BmgBI CACGTC 1 cut(s) 2069
BmgT120I GGNCC 4 cut(s) 1970, 2024, 2694, 3004
BmiI GGNNCC 3 cut(s) 1239, 1686, 2025
BmrFI CCNGG 5 cut(s) 225, 1097, 1769, 2057, 3008
BplI GAGNNNNNCTC 2 cut(s) 1229, 1261
BpmI CTGGAG 3 cut(s) 956, 1599, 2945
Bpu10I CCTNAGC 3 cut(s) 741, 2154, 2538
BpuMI CCSGG 1 cut(s) 1097
BsaHI GRCGYC 1 cut(s) 1749
BsaJI CCNNGG 6 cut(s) 224, 579, 760, 2055, 2690, 2710
BsaXI ACNNNNNCTCC 6 cut(s) 220, 250, 2064, 2094, 2706, 2736
Bsc4I CCNNNNNNNGG 6 cut(s) 1327, 1594, 1913, 1967, 2021, 2527
Bse1I ACTGG 4 cut(s) 803, 1612, 1816, 2352
Bse3DI GCAATG 5 cut(s) 735, 1657, 1972, 2597, 2790
BseBI CCWGG 4 cut(s) 225, 1769, 2057, 3008
BseDI CCNNGG 6 cut(s) 224, 579, 760, 2055, 2690, 2710
BseGI GGATG 9 cut(s) 736, 890, 1957, 2113, 2245, 2491, 2536, 2614, 2744
BseLI CCNNNNNNNGG 6 cut(s) 1327, 1594, 1913, 1967, 2021, 2527
BseMI GCAATG 5 cut(s) 735, 1657, 1972, 2597, 2790
BseMII CTCAG 7 cut(s) 111, 501, 545, 732, 1539, 1911, 2145
BseNI ACTGG 4 cut(s) 803, 1612, 1816, 2352
BseRI GAGGAG 4 cut(s) 527, 596, 617, 1910
BseXI GCAGC 6 cut(s) 982, 1132, 1541, 2251, 2529, 2614
BseYI CCCAGC 1 cut(s) 1680
BsgI GTGCAG 1 cut(s) 691
Bsh1236I CGCG 2 cut(s) 489, 1008
BshFI GGCC 4 cut(s) 328, 550, 1849, 1876
BsiHKAI GWGCWC 2 cut(s) 861, 2655
BsiHKCI CYCGRG 1 cut(s) 1158
BsiSI CCGG 1 cut(s) 1096
BslFI GGGAC 5 cut(s) 579, 1242, 1839, 2037, 2525
BslI CCNNNNNNNGG 6 cut(s) 1327, 1594, 1913, 1967, 2021, 2527
BsmAI GTCTC 3 cut(s) 1484, 1533, 2800
BsmFI GGGAC 5 cut(s) 579, 1242, 1839, 2037, 2525
BsnI GGCC 4 cut(s) 328, 550, 1849, 1876
BsoBI CYCGRG 1 cut(s) 1158
Bsp1286I GDGCHC 2 cut(s) 861, 2655
Bsp143I GATC 7 cut(s) 178, 1623, 2353, 2390, 2425, 2626, 2857
Bsp19I CCATGG 1 cut(s) 2690
BspACI CCGC 6 cut(s) 43, 1702, 1877, 2111, 2744, 2842
BspANI GGCC 4 cut(s) 328, 550, 1849, 1876
BspCNI CTCAG 7 cut(s) 112, 502, 546, 733, 1540, 1912, 2146
BspFNI CGCG 2 cut(s) 489, 1008
BspHI TCATGA 3 cut(s) 907, 2428, 2556
BspLI GGNNCC 3 cut(s) 1239, 1686, 2025
BspMAI CTGCAG 4 cut(s) 1028, 1465, 2547, 2737
BspMI ACCTGC 1 cut(s) 2596
BspPI GGATC 3 cut(s) 1631, 2348, 2398
BsrDI GCAATG 5 cut(s) 735, 1657, 1972, 2597, 2790
BsrI ACTGG 4 cut(s) 803, 1612, 1816, 2352
BssECI CCNNGG 6 cut(s) 224, 579, 760, 2055, 2690, 2710
BssMI GATC 7 cut(s) 178, 1623, 2353, 2390, 2425, 2626, 2857
BssNI GRCGYC 1 cut(s) 1749
BssSI CACGAG 1 cut(s) 1536
BssT1I CCWWGG 2 cut(s) 2690, 2710
Bst2BI CACGAG 1 cut(s) 1536
Bst2UI CCWGG 4 cut(s) 225, 1769, 2057, 3008
Bst4CI ACNGT 8 cut(s) 167, 992, 1568, 1736, 1830, 2167, 2236, 2591
Bst6I CTCTTC 6 cut(s) 19, 227, 317, 1185, 1410, 2472
BstACI GRCGYC 1 cut(s) 1749
BstAPI GCANNNNNTGC 4 cut(s) 669, 783, 1023, 1317
BstC8I GCNNGC 6 cut(s) 501, 784, 788, 1704, 2193, 2451
BstDSI CCRYGG 2 cut(s) 760, 2690
BstF5I GGATG 9 cut(s) 736, 890, 1957, 2113, 2245, 2491, 2536, 2614, 2744
BstFNI CGCG 2 cut(s) 489, 1008
BstH2I RGCGCY 1 cut(s) 1903
BstHHI GCGC 3 cut(s) 489, 792, 1902
BstKTI GATC 7 cut(s) 181, 1626, 2356, 2393, 2428, 2629, 2860
BstMAI GTCTC 3 cut(s) 1484, 1533, 2800
BstMBI GATC 7 cut(s) 178, 1623, 2353, 2390, 2425, 2626, 2857
BstMWI GCNNNNNNNGC 6 cut(s) 669, 783, 938, 1014, 1023, 1317
BstNI CCWGG 4 cut(s) 225, 1769, 2057, 3008
BstNSI RCATGY 4 cut(s) 786, 1375, 2195, 2372
BstSCI CCNGG 5 cut(s) 223, 1095, 1767, 2055, 3006
BstSFI CTRYAG 4 cut(s) 1024, 1461, 2543, 2733
BstUI CGCG 2 cut(s) 489, 1008
BstV1I GCAGC 6 cut(s) 982, 1132, 1541, 2251, 2529, 2614
BstX2I RGATCY 1 cut(s) 2390
BstXI CCANNNNNNTGG 1 cut(s) 2063
BstYI RGATCY 1 cut(s) 2390
BsuI GTATCC 3 cut(s) 619, 723, 1808
BsuRI GGCC 4 cut(s) 328, 550, 1849, 1876
BtgI CCRYGG 2 cut(s) 760, 2690
BtgZI GCGATG 1 cut(s) 947
BtrI CACGTC 1 cut(s) 2069
BtsCI GGATG 9 cut(s) 736, 890, 1957, 2113, 2245, 2491, 2536, 2614, 2744
BtsI GCAGTG 3 cut(s) 881, 1354, 2332
BtsIMutI CAGTG 7 cut(s) 881, 1354, 1619, 2133, 2332, 2587, 2913
BveI ACCTGC 1 cut(s) 2596
Cac8I GCNNGC 6 cut(s) 501, 784, 788, 1704, 2193, 2451
CaiI CAGNNNCTG 1 cut(s) 1056
CciI TCATGA 3 cut(s) 907, 2428, 2556
CfoI GCGC 3 cut(s) 489, 792, 1902
Cfr13I GGNCC 4 cut(s) 1970, 2024, 2694, 3004
Csp6I GTAC 3 cut(s) 833, 868, 1564
CspCI CAANNNNNGTGG 2 cut(s) 1839, 1874
CviQI GTAC 3 cut(s) 833, 868, 1564
DpnI GATC 7 cut(s) 180, 1625, 2355, 2392, 2427, 2628, 2859
DpnII GATC 7 cut(s) 178, 1623, 2353, 2390, 2425, 2626, 2857
DraI TTTAAA 1 cut(s) 1602
EaeI YGGCCR 2 cut(s) 1847, 1874
Eam1104I CTCTTC 6 cut(s) 19, 227, 317, 1185, 1410, 2472
EarI CTCTTC 6 cut(s) 19, 227, 317, 1185, 1410, 2472
Eco130I CCWWGG 2 cut(s) 2690, 2710
Eco147I AGGCCT 2 cut(s) 328, 550
Eco32I GATATC 3 cut(s) 1585, 1789, 2434
Eco47I GGWCC 4 cut(s) 1970, 2024, 2694, 3004
Eco57I CTGAAG 3 cut(s) 90, 836, 2157
Eco88I CYCGRG 1 cut(s) 1158
EcoRI GAATTC 2 cut(s) 903, 2573
EcoRII CCWGG 4 cut(s) 223, 1767, 2055, 3006
EcoRV GATATC 3 cut(s) 1585, 1789, 2434
EcoT14I CCWWGG 2 cut(s) 2690, 2710
EcoT22I ATGCAT 3 cut(s) 1373, 1519, 2774
ErhI CCWWGG 2 cut(s) 2690, 2710
FalI AAGNNNNNCTT 4 cut(s) 1441, 1473, 2130, 2162
FaqI GGGAC 5 cut(s) 579, 1242, 1839, 2037, 2525
FauI CCCGC 1 cut(s) 1695
FauNDI CATATG 2 cut(s) 1072, 1297
FbaI TGATCA 1 cut(s) 2626
FblI GTMKAC 1 cut(s) 2524
Fnu4HI GCNGC 8 cut(s) 44, 996, 1121, 1530, 1877, 2265, 2543, 2603
FokI GGATG 9 cut(s) 743, 877, 1944, 2120, 2252, 2498, 2543, 2601, 2751
Fsp4HI GCNGC 8 cut(s) 44, 996, 1121, 1530, 1877, 2265, 2543, 2603
FspBI CTAG 3 cut(s) 1124, 1254, 1358
GlaI GCGC 3 cut(s) 488, 791, 1901
GluI GCNGC 8 cut(s) 44, 996, 1121, 1530, 1877, 2265, 2543, 2603
GsaI CCCAGC 1 cut(s) 1684
GsuI CTGGAG 3 cut(s) 956, 1599, 2945
HaeII RGCGCY 1 cut(s) 1903
HaeIII GGCC 4 cut(s) 328, 550, 1849, 1876
HapII CCGG 1 cut(s) 1096
HhaI GCGC 3 cut(s) 489, 792, 1902
Hin1I GRCGYC 1 cut(s) 1749
Hin6I GCGC 3 cut(s) 487, 790, 1900
HinP1I GCGC 3 cut(s) 487, 790, 1900
HindIII AAGCTT 3 cut(s) 1428, 2087, 2663
HinfI GANTC 4 cut(s) 1102, 2075, 2273, 2480
HpaII CCGG 1 cut(s) 1096
Hpy166II GTNNAC 4 cut(s) 406, 1739, 2143, 2525
Hpy8I GTNNAC 4 cut(s) 406, 1739, 2143, 2525
HpyCH4III ACNGT 8 cut(s) 167, 992, 1568, 1736, 1830, 2167, 2236, 2591
HpyCH4IV ACGT 3 cut(s) 1389, 1749, 2068
HpyF10VI GCNNNNNNNGC 6 cut(s) 669, 783, 938, 1014, 1023, 1317
HpySE526I ACGT 3 cut(s) 1389, 1749, 2068
Hsp92I GRCGYC 1 cut(s) 1749
HspAI GCGC 3 cut(s) 487, 790, 1900
Ksp22I TGATCA 1 cut(s) 2626
Kzo9I GATC 7 cut(s) 178, 1623, 2353, 2390, 2425, 2626, 2857
LmnI GCTCC 7 cut(s) 559, 583, 975, 1237, 1897, 2059, 2650
Lsp1109I GCAGC 6 cut(s) 982, 1132, 1541, 2251, 2529, 2614
MaeI CTAG 3 cut(s) 1124, 1254, 1358
MaeII ACGT 3 cut(s) 1389, 1749, 2068
MaeIII GTNAC 5 cut(s) 427, 1138, 2080, 2911, 2959
MalI GATC 7 cut(s) 180, 1625, 2355, 2392, 2427, 2628, 2859
MboI GATC 7 cut(s) 178, 1623, 2353, 2390, 2425, 2626, 2857
MfeI CAATTG 4 cut(s) 772, 1115, 2224, 2358
MflI RGATCY 1 cut(s) 2390
MhlI GDGCHC 2 cut(s) 861, 2655
MlsI TGGCCA 1 cut(s) 1849
MluNI TGGCCA 1 cut(s) 1849
MlyI GAGTC 1 cut(s) 2489
MmeI TCCRAC 4 cut(s) 2085, 2353, 2454, 2463
Mox20I TGGCCA 1 cut(s) 1849
Mph1103I ATGCAT 3 cut(s) 1373, 1519, 2774
MroXI GAANNNNTTC 1 cut(s) 2564
MscI TGGCCA 1 cut(s) 1849
MseI TTAA 6 cut(s) 807, 872, 1601, 1676, 2304, 2667
MslI CAYNNNNRTG 2 cut(s) 1285, 2138
Msp20I TGGCCA 1 cut(s) 1849
MspI CCGG 1 cut(s) 1096
MspR9I CCNGG 5 cut(s) 225, 1097, 1769, 2057, 3008
MunI CAATTG 4 cut(s) 772, 1115, 2224, 2358
MvaI CCWGG 4 cut(s) 225, 1769, 2057, 3008
MvnI CGCG 2 cut(s) 489, 1008
MwoI GCNNNNNNNGC 6 cut(s) 669, 783, 938, 1014, 1023, 1317
NciI CCSGG 1 cut(s) 1097
NcoI CCATGG 1 cut(s) 2690
NdeI CATATG 2 cut(s) 1072, 1297
NdeII GATC 7 cut(s) 178, 1623, 2353, 2390, 2425, 2626, 2857
NlaIV GGNNCC 3 cut(s) 1239, 1686, 2025
NmuCI GTSAC 4 cut(s) 427, 1138, 2911, 2959
NsiI ATGCAT 3 cut(s) 1373, 1519, 2774
NspI RCATGY 4 cut(s) 786, 1375, 2195, 2372
OliI CACNNNNGTG 1 cut(s) 2138
PaeI GCATGC 2 cut(s) 786, 2195
PagI TCATGA 3 cut(s) 907, 2428, 2556
PceI AGGCCT 2 cut(s) 328, 550
PciI ACATGT 1 cut(s) 2368
PdmI GAANNNNTTC 1 cut(s) 2564
PfeI GAWTC 3 cut(s) 1102, 2075, 2273
PflFI GACNNNGTC 1 cut(s) 2792
PfoI TCCNGGA 1 cut(s) 3006
PkrI GCNGC 8 cut(s) 45, 997, 1122, 1531, 1878, 2266, 2544, 2604
PleI GAGTC 1 cut(s) 2488
PpsI GAGTC 1 cut(s) 2488
PscI ACATGT 1 cut(s) 2368
Psp6I CCWGG 4 cut(s) 223, 1767, 2055, 3006
PspFI CCCAGC 1 cut(s) 1680
PspGI CCWGG 4 cut(s) 223, 1767, 2055, 3006
PspN4I GGNNCC 3 cut(s) 1239, 1686, 2025
PspPI GGNCC 4 cut(s) 1970, 2024, 2694, 3004
PstI CTGCAG 4 cut(s) 1028, 1465, 2547, 2737
PstNI CAGNNNCTG 1 cut(s) 1056
PsuI RGATCY 1 cut(s) 2390
PsyI GACNNNGTC 1 cut(s) 2792
RsaI GTAC 3 cut(s) 834, 869, 1565
RsaNI GTAC 3 cut(s) 833, 868, 1564
RseI CAYNNNNRTG 2 cut(s) 1285, 2138
SaqAI TTAA 6 cut(s) 807, 872, 1601, 1676, 2304, 2667
SatI GCNGC 8 cut(s) 44, 996, 1121, 1530, 1877, 2265, 2543, 2603
Sau3AI GATC 7 cut(s) 178, 1623, 2353, 2390, 2425, 2626, 2857
Sau96I GGNCC 4 cut(s) 1970, 2024, 2694, 3004
SbfI CCTGCAGG 1 cut(s) 2737
ScaI AGTACT 2 cut(s) 834, 869
SchI GAGTC 1 cut(s) 2489
ScrFI CCNGG 5 cut(s) 225, 1097, 1769, 2057, 3008
SdaI CCTGCAGG 1 cut(s) 2737
SduI GDGCHC 2 cut(s) 861, 2655
SfcI CTRYAG 4 cut(s) 1024, 1461, 2543, 2733
SinI GGWCC 4 cut(s) 1970, 2024, 2694, 3004
SmiMI CAYNNNNRTG 2 cut(s) 1285, 2138
SpeI ACTAGT 1 cut(s) 1253
SphI GCATGC 2 cut(s) 786, 2195
Sse8387I CCTGCAGG 1 cut(s) 2737
SseBI AGGCCT 2 cut(s) 328, 550
SsiI CCGC 6 cut(s) 43, 1702, 1877, 2111, 2744, 2842
SspMI CTAG 3 cut(s) 1124, 1254, 1358
StuI AGGCCT 2 cut(s) 328, 550
StyD4I CCNGG 5 cut(s) 223, 1095, 1767, 2055, 3006
StyI CCWWGG 2 cut(s) 2690, 2710
TaaI ACNGT 8 cut(s) 167, 992, 1568, 1736, 1830, 2167, 2236, 2591
TaiI ACGT 3 cut(s) 1392, 1752, 2071
TaqI TCGA 3 cut(s) 1105, 1491, 1505
TatI WGTACW 3 cut(s) 832, 867, 1563
TauI GCSGC 2 cut(s) 46, 1879
TfiI GAWTC 3 cut(s) 1102, 2075, 2273
Tru1I TTAA 6 cut(s) 807, 872, 1601, 1676, 2304, 2667
Tru9I TTAA 6 cut(s) 807, 872, 1601, 1676, 2304, 2667
TscAI CASTG 7 cut(s) 888, 1354, 1619, 2140, 2332, 2594, 2920
TseFI GTSAC 4 cut(s) 427, 1138, 2911, 2959
TseI GCWGC 6 cut(s) 995, 1120, 1529, 2264, 2542, 2602
Tsp45I GTSAC 4 cut(s) 427, 1138, 2911, 2959
TspGWI ACGGA 2 cut(s) 349, 2510
TspRI CASTG 7 cut(s) 888, 1354, 1619, 2140, 2332, 2594, 2920
Tth111I GACNNNGTC 1 cut(s) 2792
VpaK11BI GGWCC 4 cut(s) 1970, 2024, 2694, 3004
XapI RAATTY 4 cut(s) 903, 2214, 2457, 2573
XbaI TCTAGA 1 cut(s) 1357
XceI RCATGY 4 cut(s) 786, 1375, 2195, 2372
XcmI CCANNNNNNNNNTGG 2 cut(s) 1695, 1808
XmiI GTMKAC 1 cut(s) 2524
XmnI GAANNNNTTC 1 cut(s) 2564
XspI CTAG 3 cut(s) 1124, 1254, 1358
ZraI GACGTC 1 cut(s) 1750
ZrmI AGTACT 2 cut(s) 834, 869
Zsp2I ATGCAT 3 cut(s) 1373, 1519, 2774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.