Rw1G016730

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000280
Physical Location & Seq
Reverse (-)
37256574 .. 37264179
7606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G016730.1

Sequence Viewer

Length: 2625 bp
ATGAAGAGAGACGGCGTAAAGCAAGCTTATGATGACCCAGAACCCACATTTCCAGAGAAAGACGAGGGCTTTTTAGAAAACCAGAGTCACATGGCTGAGATTGTGCAGAAGAAGCGTAGTACCAGGAAGAGAGCTACAGGGAAGGGATTGGCAGCCGTGAATGGCCCAAGGGATTGTGAGAAAGGAATTCTATCATGTGCCGCCAACTGGGGTCGGAAGCGGTTTAGGCGCGTGAGACCACGCAATGCTGCAAAGTGGATTGAGGATTGTTTCGAGTGCCTTCACTGCCAGGGGAATGATGAGGGCAGAGTTGTTTGCTGTGGAAGTTGCAAAAGAAAATGGTATCCTCAGATGTTAGAGGATGCCATTGCTGAGGCTTGTCCTGGATGCTGTGGTAATTGCAGTTGCAAAGCATGTTCGTGTGCAGCTTCTAGAAATTTGAAGAAACAGCCGAAGTTGAATATTAGCAAAGAGGAAAAACTCGAGCACTCTAAGTATTTGCTTCAAGCATTGCTTCCACTATTAAAACGACTTAACAATGAGCAAGTGATTGAGATGGAGATGGAGGCTAGGAGACAAGGGCTGTCTGTTTCGGAGTTAAAGATCCAAAAGTCAAACTGCTCTTATGATGAGCGTGTGAATTGTAACAAATGCAAAGCTTCAATTGTTGATTTGTATAGAAGCTGCAATTTATGCTCATATGATCTCTGCCTAACGTGTTGCCGGGAGAGTCGAGATCGACACCTGGATGGAGGTGGGAAGGAAGTGACTATGGTGTATATCAATCAGGACCTAGAACATTTGCATGGTGGAAAGGGGAACATAGAAGAACTACTGTTTGAACCTAGCCCCAAGTGTCATGTAAGGTTAAAATCTGAATGGAAATCCAATGAAGATGGTAGCATCCTTTGCCCTCCAGAGGATAGGGATGGCTGTGGTCATGGTCATCTGGAATTGAGATGCATGTCTTTTGAGAATAAGGTTGGTGAGTTGGTACAGAAAGCAGAAGAGATGGCTGAAATTTACAAACTTATGCATGCGGCAGAAACTTCCGCACAGTGGTGTTCATGTTTCAATTCTTTGGATGTTTTTGATTCAATCAGCAACACCAAAAAGTTAAGGAAAGGAGATTCTCAAGAGGATTTTGATGACAACTATTTGTACTGCCCAAGAGCTGGAAATATCCAACGTGAGGATCTTAAGCATTTCCAATGGCATTGGCTCAGGGGTGAGCCTGTGATTGTTAGCAATGTAATTGAAACTACATCATGTATGTGGCCTGCTTGTCGTCAGATGCAACAGACTATGCATGGTAAATATTTAACAATCAAGGCCATTGATTGCCTGGATTGGTGCAAGCAAGATATCAGTATCCATGAATTTTTTACTGGATATGTAAAGGGGTGGTTTGATTGGAAAATGGGGCCTCAAAGGCTAAAACTGAGAGATTGTGCTCCAGCGAGTTTGATTGAGGAGCACCTTCCTCATCATGGTGCTGAGTTTATCTATGGCTTGCCATTTAAGGAATATACTCATCCTCACAGTGGGTTTCTTAATCTTGCTGCTAAATTCCCTGAGGAGTGTGGGAAGCCTGCTTATGGCGTTGGGCAGGAGCTTGGACGTGAAGATTCTGTGACTAAGCTTCATTGCAATATATCTGATGTGGTGAATGTTTTGACACATACTGCTGAAGTGACCCCTAATTCTCAACAACTTCGTACAGTAGAAAGGCTAAAAAAAGCGCAGATTGAGCAAGATAGAAGAGAAACTTTAGGAAACTGTCACTCTGCGGATGGCAATGTTGATGGTGGAAATCTTTCAAACTATTCCAGATCTGAAAATAATGTAGACGGTGGTGCTTTATGGGACATTTTTCGGAGACAGGATGTTCCAAAACTACAGGAATATCTCAGGAAGCACTTCAACGAGTTCAGTCACACATACTGCTGTCCGTCCCAACAGATTATTCATCCCATTCACGATCAGAACTTTTACCTGACTCGGGAGCACAAAAGGAAGCTGAAAGAGGAATGTGGAATCGAACCATGGACATGTATCCAGAAACTTGGGGATGCTATCTTTGTTCCTGCAGGCTGTCCATATCAAGTCAGAAACTTGAAGTCATGCATTAAAGTTGCAGTTCAATTTGTCTCACCTGAAAGTGTTGGCGATTGCTTTCGTTTGACTGAAGAATGCCGCACACTTCCAAAAGATCACGAGGCTAAAGAGGATAAATTGGAGGTGAAAAAAATGATTGTGCATGCTATGCGGGAAGCTGTGGATATTTTGGATCAGAGTGTGAGGGTTAGACAAGCAAAGAGAGAGGAAGTTGGACAAAGACAGCAAGTACAGAGCAGAAAGGAGGGGGAAGTTCAAGTTGATGAGGAGCAGCCAACAATGTCATCACATCCAGCACAGTTCCCTCTTGATCTTGTTACAGCAATGCAGCGGCTAGATGACCTTGTGGAGCGCTTTTCGGATTTTGAACACTTGGAAGAGAGAGATAGAATAATGAATCCGGAATACCAGTTCTTATGTTCAGAGTTCATGAATTTATCTTCACATCTTGGTTATGCAACTCAGGTGAAAGAGTCTGCCATTCCACCATCCTATCAGCAACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

874

Amino Acids

100.28

Weight (kDa)

6.39

Isoelectric Point (pI)

57.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjC PF02373 614 - 716 2.7e-13 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1175
AccI GTMKAC 1 cut(s) 1848
AccII CGCG 1 cut(s) 231
AccIII TCCGGA 1 cut(s) 2518
AciI CCGC 8 cut(s) 201, 220, 1040, 1053, 1790, 2197, 2269, 2449
AclWI GGATC 3 cut(s) 598, 1203, 2298
AcsI RAATTY 6 cut(s) 186, 436, 1020, 1379, 1568, 2551
AcuI CTGAAG 2 cut(s) 1710, 2208
AfaI GTAC 5 cut(s) 121, 996, 1163, 1720, 2349
AfeI AGCGCT 1 cut(s) 2471
AfiI CCNNNNNNNGG 9 cut(s) 207, 919, 1059, 1175, 1192, 1490, 1544, 1598, 2002
AflII CTTAAG 1 cut(s) 1199
AflIII ACRYGT 2 cut(s) 716, 2051
AjiI CACGTC 1 cut(s) 1622
AjnI CCWGG 5 cut(s) 122, 288, 382, 744, 1344
AjuI GAANNNNNNNTTGG 2 cut(s) 1950, 1982
AleI CACNNNNGTG 1 cut(s) 1060
AloI GAACNNNNNNTCC 2 cut(s) 2513, 2545
Alw21I GWGCWC 4 cut(s) 489, 1456, 1479, 2010
Alw26I GTCTC 5 cut(s) 3, 229, 568, 1873, 2155
AlwI GGATC 3 cut(s) 598, 1203, 2298
Ama87I CYCGRG 2 cut(s) 482, 2001
Aor13HI TCCGGA 1 cut(s) 2518
Aor51HI AGCGCT 1 cut(s) 2471
AoxI GGCC 4 cut(s) 163, 1277, 1332, 1424
ApeKI GCWGC 7 cut(s) 152, 248, 425, 684, 1562, 2389, 2446
ApoI RAATTY 6 cut(s) 186, 436, 1020, 1379, 1568, 2551
Asp700I GAANNNNTTC 2 cut(s) 1816, 1919
AspLEI GCGC 3 cut(s) 231, 1744, 2472
AspS9I GGNCC 3 cut(s) 164, 790, 1424
AsuC2I CCSGG 1 cut(s) 725
AsuHPI GGTGA 6 cut(s) 998, 1241, 1678, 2145, 2254, 2596
AvaI CYCGRG 2 cut(s) 482, 2001
AvaII GGWCC 1 cut(s) 790
AxyI CCTNAGG 1 cut(s) 1575
BauI CACGAG 1 cut(s) 2216
Bbv12I GWGCWC 4 cut(s) 489, 1456, 1479, 2010
BbvCI CCTCAGC 1 cut(s) 372
BbvI GCAGC 7 cut(s) 164, 235, 437, 671, 1549, 2401, 2458
BccI CCATC 9 cut(s) 550, 556, 743, 890, 923, 1006, 1787, 1799, 2614
BceAI ACGGC 2 cut(s) 28, 140
BciT130I CCWGG 5 cut(s) 124, 290, 384, 746, 1346
BciVI GTATCC 3 cut(s) 354, 1382, 2066
BcnI CCSGG 1 cut(s) 725
BcoDI GTCTC 5 cut(s) 3, 229, 568, 1873, 2155
BfaI CTAG 5 cut(s) 432, 570, 794, 846, 2453
BfmI CTRYAG 3 cut(s) 135, 1898, 2088
BfoI RGCGCY 1 cut(s) 2473
BfrI CTTAAG 1 cut(s) 1199
BfuI GTATCC 3 cut(s) 354, 1382, 2066
BglI GCCNNNNNGGC 1 cut(s) 1432
BglII AGATCT 1 cut(s) 1832
Bme1390I CCNGG 6 cut(s) 124, 290, 384, 725, 746, 1346
Bme18I GGWCC 1 cut(s) 790
BmeT110I CYCGRG 2 cut(s) 482, 2001
BmgBI CACGTC 1 cut(s) 1622
BmgT120I GGNCC 3 cut(s) 164, 790, 1424
BmiI GGNNCC 1 cut(s) 1425
BmrFI CCNGG 6 cut(s) 124, 290, 384, 725, 746, 1346
BmrI ACTGGG 1 cut(s) 217
BmsI GCATC 6 cut(s) 352, 377, 912, 950, 1284, 2062
BmuI ACTGGG 1 cut(s) 217
BpmI CTGGAG 2 cut(s) 900, 1440
Bpu10I CCTNAGC 2 cut(s) 372, 1223
BpuEI CTTGAG 1 cut(s) 1119
BpuMI CCSGG 1 cut(s) 725
BsaI GGTCTC 1 cut(s) 229
BsaJI CCNNGG 3 cut(s) 167, 289, 2045
BsaWI WCCGGW 1 cut(s) 2518
Bsc4I CCNNNNNNNGG 9 cut(s) 207, 919, 1059, 1175, 1192, 1490, 1544, 1598, 2002
Bse1I ACTGG 3 cut(s) 212, 1393, 2527
Bse21I CCTNAGG 1 cut(s) 1575
Bse3DI GCAATG 7 cut(s) 250, 366, 509, 1255, 1645, 1804, 2448
BseAI TCCGGA 1 cut(s) 2518
BseBI CCWGG 5 cut(s) 124, 290, 384, 746, 1346
BseDI CCNNGG 3 cut(s) 167, 289, 2045
BseLI CCNNNNNNNGG 9 cut(s) 207, 919, 1059, 1175, 1192, 1490, 1544, 1598, 2002
BseMI GCAATG 7 cut(s) 250, 366, 509, 1255, 1645, 1804, 2448
BseMII CTCAG 9 cut(s) 87, 362, 363, 1237, 1433, 1488, 1566, 1924, 2594
BseNI ACTGG 3 cut(s) 212, 1393, 2527
BseRI GAGGAG 3 cut(s) 1487, 1592, 2399
BseXI GCAGC 7 cut(s) 164, 235, 437, 671, 1549, 2401, 2458
BsgI GTGCAG 2 cut(s) 125, 444
Bsh1236I CGCG 1 cut(s) 231
BshFI GGCC 4 cut(s) 165, 1279, 1334, 1426
BsiHKAI GWGCWC 4 cut(s) 489, 1456, 1479, 2010
BsiHKCI CYCGRG 2 cut(s) 482, 2001
BsiSI CCGG 2 cut(s) 724, 2519
BslFI GGGAC 2 cut(s) 1880, 1939
BslI CCNNNNNNNGG 9 cut(s) 207, 919, 1059, 1175, 1192, 1490, 1544, 1598, 2002
BsmAI GTCTC 5 cut(s) 3, 229, 568, 1873, 2155
BsmBI CGTCTC 1 cut(s) 3
BsmFI GGGAC 2 cut(s) 1880, 1939
BsmI GAATGC 1 cut(s) 2198
BsnI GGCC 4 cut(s) 165, 1279, 1334, 1426
Bso31I GGTCTC 1 cut(s) 229
BsoBI CYCGRG 2 cut(s) 482, 2001
Bsp1286I GDGCHC 4 cut(s) 489, 1456, 1479, 2010
Bsp13I TCCGGA 1 cut(s) 2518
Bsp143I GATC 9 cut(s) 603, 703, 736, 1195, 1832, 1981, 2212, 2290, 2428
Bsp19I CCATGG 1 cut(s) 2045
BspACI CCGC 8 cut(s) 201, 220, 1040, 1053, 1790, 2197, 2269, 2449
BspANI GGCC 4 cut(s) 165, 1279, 1334, 1426
BspCNI CTCAG 9 cut(s) 88, 361, 364, 1236, 1434, 1489, 1567, 1923, 2593
BspEI TCCGGA 1 cut(s) 2518
BspFNI CGCG 1 cut(s) 231
BspHI TCATGA 1 cut(s) 2547
BspLI GGNNCC 1 cut(s) 1425
BspMAI CTGCAG 1 cut(s) 2092
BspPI GGATC 3 cut(s) 598, 1203, 2298
BspTI CTTAAG 1 cut(s) 1199
BspTNI GGTCTC 1 cut(s) 229
BsrDI GCAATG 7 cut(s) 250, 366, 509, 1255, 1645, 1804, 2448
BsrI ACTGG 3 cut(s) 212, 1393, 2527
BssECI CCNNGG 3 cut(s) 167, 289, 2045
BssMI GATC 9 cut(s) 603, 703, 736, 1195, 1832, 1981, 2212, 2290, 2428
BssSI CACGAG 1 cut(s) 2216
BssT1I CCWWGG 2 cut(s) 167, 2045
Bst2BI CACGAG 1 cut(s) 2216
Bst2UI CCWGG 5 cut(s) 124, 290, 384, 746, 1346
Bst4CI ACNGT 7 cut(s) 837, 1059, 1544, 1723, 1781, 1853, 2418
Bst6I CTCTTC 4 cut(s) 122, 1002, 1756, 2490
BstAFI CTTAAG 1 cut(s) 1199
BstAPI GCANNNNNTGC 3 cut(s) 693, 909, 2266
BstC8I GCNNGC 8 cut(s) 24, 1038, 1281, 1358, 1514, 1593, 2092, 2262
BstDSI CCRYGG 1 cut(s) 2045
BstFNI CGCG 1 cut(s) 231
BstH2I RGCGCY 1 cut(s) 2473
BstHHI GCGC 3 cut(s) 231, 1744, 2472
BstKTI GATC 9 cut(s) 606, 706, 739, 1198, 1835, 1984, 2215, 2293, 2431
BstMAI GTCTC 5 cut(s) 3, 229, 568, 1873, 2155
BstMBI GATC 9 cut(s) 603, 703, 736, 1195, 1832, 1981, 2212, 2290, 2428
BstMWI GCNNNNNNNGC 8 cut(s) 112, 226, 285, 693, 909, 1432, 1750, 2266
BstNI CCWGG 5 cut(s) 124, 290, 384, 746, 1346
BstNSI RCATGY 5 cut(s) 417, 967, 1040, 2055, 2264
BstSCI CCNGG 6 cut(s) 122, 288, 382, 723, 744, 1344
BstSFI CTRYAG 3 cut(s) 135, 1898, 2088
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 7 cut(s) 164, 235, 437, 671, 1549, 2401, 2458
BstX2I RGATCY 3 cut(s) 603, 1195, 1832
BstXI CCANNNNNNTGG 1 cut(s) 2066
BstYI RGATCY 3 cut(s) 603, 1195, 1832
Bsu36I CCTNAGG 1 cut(s) 1575
BsuI GTATCC 3 cut(s) 354, 1382, 2066
BsuRI GGCC 4 cut(s) 165, 1279, 1334, 1426
BtgI CCRYGG 1 cut(s) 2045
BtrI CACGTC 1 cut(s) 1622
BtsI GCAGTG 1 cut(s) 283
BtsIMutI CAGTG 3 cut(s) 283, 1064, 1549
Cac8I GCNNGC 8 cut(s) 24, 1038, 1281, 1358, 1514, 1593, 2092, 2262
CciI TCATGA 1 cut(s) 2547
CfoI GCGC 3 cut(s) 231, 1744, 2472
Cfr13I GGNCC 3 cut(s) 164, 790, 1424
Csp6I GTAC 5 cut(s) 120, 995, 1162, 1719, 2348
CviQI GTAC 5 cut(s) 120, 995, 1162, 1719, 2348
DpnI GATC 9 cut(s) 605, 705, 738, 1197, 1834, 1983, 2214, 2292, 2430
DpnII GATC 9 cut(s) 603, 703, 736, 1195, 1832, 1981, 2212, 2290, 2428
Eam1104I CTCTTC 4 cut(s) 122, 1002, 1756, 2490
EarI CTCTTC 4 cut(s) 122, 1002, 1756, 2490
Eco130I CCWWGG 2 cut(s) 167, 2045
Eco31I GGTCTC 1 cut(s) 229
Eco32I GATATC 1 cut(s) 1366
Eco47I GGWCC 1 cut(s) 790
Eco47III AGCGCT 1 cut(s) 2471
Eco57I CTGAAG 2 cut(s) 1710, 2208
Eco81I CCTNAGG 1 cut(s) 1575
Eco88I CYCGRG 2 cut(s) 482, 2001
EcoO109I RGGNCCY 2 cut(s) 790, 1424
EcoRI GAATTC 1 cut(s) 186
EcoRII CCWGG 5 cut(s) 122, 288, 382, 744, 1344
EcoRV GATATC 1 cut(s) 1366
EcoT14I CCWWGG 2 cut(s) 167, 2045
EcoT22I ATGCAT 4 cut(s) 965, 1038, 1311, 2129
ErhI CCWWGG 2 cut(s) 167, 2045
Esp3I CGTCTC 1 cut(s) 3
FalI AAGNNNNNCTT 4 cut(s) 498, 530, 1753, 1785
FaqI GGGAC 2 cut(s) 1880, 1939
FauI CCCGC 1 cut(s) 2262
FauNDI CATATG 1 cut(s) 700
FblI GTMKAC 1 cut(s) 1848
FspBI CTAG 5 cut(s) 432, 570, 794, 846, 2453
GlaI GCGC 3 cut(s) 230, 1743, 2471
GsuI CTGGAG 2 cut(s) 900, 1440
HaeII RGCGCY 1 cut(s) 2473
HaeIII GGCC 4 cut(s) 165, 1279, 1334, 1426
HapII CCGG 2 cut(s) 724, 2519
HhaI GCGC 3 cut(s) 231, 1744, 2472
Hin6I GCGC 3 cut(s) 229, 1742, 2470
HinP1I GCGC 3 cut(s) 229, 1742, 2470
HindIII AAGCTT 3 cut(s) 24, 657, 1640
HinfI GANTC 9 cut(s) 85, 730, 1094, 1130, 1628, 1999, 2037, 2515, 2591
HpaII CCGG 2 cut(s) 724, 2519
HphI GGTGA 6 cut(s) 998, 1241, 1678, 2145, 2254, 2596
Hpy166II GTNNAC 1 cut(s) 1849
Hpy8I GTNNAC 1 cut(s) 1849
HpyAV CCTTC 4 cut(s) 136, 290, 754, 1490
HpyCH4III ACNGT 7 cut(s) 837, 1059, 1544, 1723, 1781, 1853, 2418
HpyCH4IV ACGT 3 cut(s) 716, 1189, 1621
HpyF10VI GCNNNNNNNGC 8 cut(s) 112, 226, 285, 693, 909, 1432, 1750, 2266
HpySE526I ACGT 3 cut(s) 716, 1189, 1621
HspAI GCGC 3 cut(s) 229, 1742, 2470
Kpn2I TCCGGA 1 cut(s) 2518
Kzo9I GATC 9 cut(s) 603, 703, 736, 1195, 1832, 1981, 2212, 2290, 2428
LmnI GCTCC 6 cut(s) 1459, 1474, 1612, 2005, 2386, 2467
Lsp1109I GCAGC 7 cut(s) 164, 235, 437, 671, 1549, 2401, 2458
LweI GCATC 6 cut(s) 352, 377, 912, 950, 1284, 2062
MaeI CTAG 5 cut(s) 432, 570, 794, 846, 2453
MaeII ACGT 3 cut(s) 716, 1189, 1621
MaeIII GTNAC 8 cut(s) 86, 644, 766, 1633, 1693, 1781, 1934, 2434
MalI GATC 9 cut(s) 605, 705, 738, 1197, 1834, 1983, 2214, 2292, 2430
MboI GATC 9 cut(s) 603, 703, 736, 1195, 1832, 1981, 2212, 2290, 2428
MfeI CAATTG 1 cut(s) 663
MflI RGATCY 3 cut(s) 603, 1195, 1832
MhlI GDGCHC 4 cut(s) 489, 1456, 1479, 2010
MlyI GAGTC 4 cut(s) 94, 739, 1993, 2600
MmeI TCCRAC 3 cut(s) 194, 1210, 2311
Mph1103I ATGCAT 4 cut(s) 965, 1038, 1311, 2129
MroI TCCGGA 1 cut(s) 2518
MroXI GAANNNNTTC 2 cut(s) 1816, 1919
MslI CAYNNNNRTG 7 cut(s) 418, 747, 804, 1060, 1273, 1491, 2050
MspA1I CMGCKG 1 cut(s) 2449
MspCI CTTAAG 1 cut(s) 1199
MspI CCGG 2 cut(s) 724, 2519
MspR9I CCNGG 6 cut(s) 124, 290, 384, 725, 746, 1346
MunI CAATTG 1 cut(s) 663
Mva1269I GAATGC 1 cut(s) 2198
MvaI CCWGG 5 cut(s) 124, 290, 384, 746, 1346
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 8 cut(s) 112, 226, 285, 693, 909, 1432, 1750, 2266
NciI CCSGG 1 cut(s) 725
NcoI CCATGG 1 cut(s) 2045
NdeI CATATG 1 cut(s) 700
NdeII GATC 9 cut(s) 603, 703, 736, 1195, 1832, 1981, 2212, 2290, 2428
NlaIV GGNNCC 1 cut(s) 1425
NmuCI GTSAC 6 cut(s) 86, 766, 1633, 1693, 1781, 1934
NsiI ATGCAT 4 cut(s) 965, 1038, 1311, 2129
NspI RCATGY 5 cut(s) 417, 967, 1040, 2055, 2264
OliI CACNNNNGTG 1 cut(s) 1060
PaeI GCATGC 2 cut(s) 1040, 2264
PaeR7I CTCGAG 1 cut(s) 482
PagI TCATGA 1 cut(s) 2547
PciI ACATGT 1 cut(s) 2051
PctI GAATGC 1 cut(s) 2198
PdmI GAANNNNTTC 2 cut(s) 1816, 1919
PfeI GAWTC 5 cut(s) 1094, 1130, 1628, 2037, 2515
PflMI CCANNNNNTGG 1 cut(s) 1175
PfoI TCCNGGA 1 cut(s) 382
PleI GAGTC 4 cut(s) 93, 738, 1993, 2599
PpsI GAGTC 4 cut(s) 93, 738, 1993, 2599
PpuMI RGGWCCY 1 cut(s) 790
PscI ACATGT 1 cut(s) 2051
Psp5II RGGWCCY 1 cut(s) 790
Psp6I CCWGG 5 cut(s) 122, 288, 382, 744, 1344
PspGI CCWGG 5 cut(s) 122, 288, 382, 744, 1344
PspN4I GGNNCC 1 cut(s) 1425
PspPI GGNCC 3 cut(s) 164, 790, 1424
PspPPI RGGWCCY 1 cut(s) 790
PspXI VCTCGAGB 1 cut(s) 482
PstI CTGCAG 1 cut(s) 2092
PsuI RGATCY 3 cut(s) 603, 1195, 1832
RsaI GTAC 5 cut(s) 121, 996, 1163, 1720, 2349
RsaNI GTAC 5 cut(s) 120, 995, 1162, 1719, 2348
RseI CAYNNNNRTG 7 cut(s) 418, 747, 804, 1060, 1273, 1491, 2050
Sau3AI GATC 9 cut(s) 603, 703, 736, 1195, 1832, 1981, 2212, 2290, 2428
Sau96I GGNCC 3 cut(s) 164, 790, 1424
SbfI CCTGCAGG 1 cut(s) 2092
SchI GAGTC 4 cut(s) 94, 739, 1993, 2600
ScrFI CCNGG 6 cut(s) 124, 290, 384, 725, 746, 1346
SdaI CCTGCAGG 1 cut(s) 2092
SduI GDGCHC 4 cut(s) 489, 1456, 1479, 2010
SfaNI GCATC 6 cut(s) 352, 377, 912, 950, 1284, 2062
SfcI CTRYAG 3 cut(s) 135, 1898, 2088
Sfr274I CTCGAG 1 cut(s) 482
SinI GGWCC 1 cut(s) 790
SlaI CTCGAG 1 cut(s) 482
SmiMI CAYNNNNRTG 7 cut(s) 418, 747, 804, 1060, 1273, 1491, 2050
SmlI CTYRAG 3 cut(s) 482, 1134, 1199
SmoI CTYRAG 3 cut(s) 482, 1134, 1199
SphI GCATGC 2 cut(s) 1040, 2264
Sse8387I CCTGCAGG 1 cut(s) 2092
SsiI CCGC 8 cut(s) 201, 220, 1040, 1053, 1790, 2197, 2269, 2449
SspI AATATT 2 cut(s) 463, 1319
SspMI CTAG 5 cut(s) 432, 570, 794, 846, 2453
StyD4I CCNGG 6 cut(s) 122, 288, 382, 723, 744, 1344
StyI CCWWGG 2 cut(s) 167, 2045
TaaI ACNGT 7 cut(s) 837, 1059, 1544, 1723, 1781, 1853, 2418
TaiI ACGT 3 cut(s) 719, 1192, 1624
TaqI TCGA 5 cut(s) 273, 483, 733, 739, 2040
TatI WGTACW 2 cut(s) 1161, 2347
TauI GCSGC 4 cut(s) 203, 1043, 2199, 2452
TfiI GAWTC 5 cut(s) 1094, 1130, 1628, 2037, 2515
TscAI CASTG 3 cut(s) 290, 1064, 1549
TseFI GTSAC 6 cut(s) 86, 766, 1633, 1693, 1781, 1934
TseI GCWGC 7 cut(s) 152, 248, 425, 684, 1562, 2389, 2446
Tsp45I GTSAC 6 cut(s) 86, 766, 1633, 1693, 1781, 1934
TspDTI ATGAA 9 cut(s) 17, 906, 1056, 1392, 1634, 1958, 2528, 2536, 2564
TspGWI ACGGA 1 cut(s) 1941
TspRI CASTG 3 cut(s) 290, 1064, 1549
Van91I CCANNNNNTGG 1 cut(s) 1175
Vha464I CTTAAG 1 cut(s) 1199
VpaK11BI GGWCC 1 cut(s) 790
XapI RAATTY 6 cut(s) 186, 436, 1020, 1379, 1568, 2551
XbaI TCTAGA 1 cut(s) 431
XceI RCATGY 5 cut(s) 417, 967, 1040, 2055, 2264
XcmI CCANNNNNNNNNTGG 1 cut(s) 1342
XhoI CTCGAG 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 1848
XmnI GAANNNNTTC 2 cut(s) 1816, 1919
XspI CTAG 5 cut(s) 432, 570, 794, 846, 2453
Zsp2I ATGCAT 4 cut(s) 965, 1038, 1311, 2129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.