RchiOBHm_Chr7g0223931

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000280
Physical Location & Seq
Forward (+)
45827902 .. 45832484
4583 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20051

Sequence Viewer

Length: 4461 bp
ATGGAATTCCAGGCCACGGCGGCGAGATTGAGGCCCCGAGGCACAAAGCGGCCGAACGGCGGTGGTTTGGTGGAGAAGCAGGCCGAGGGTATTTTGGGAATTCAGAACGACGACGGAACAATTGGAGAAGATGTGGAGCCAGCAGCGCCGAAGCTTGTTCCCTGGGGGAGGAAGCGGCGGAGGACAGGATGCGAGGGTATTTTGGGAAATCAAAATGAAGGAGAGGCAAGGGCGGCAAATTGGTGCACAAGGGCTCCCTTGGACGTGGGGAAATGTTGCCATCAATGTAAGCGAAATTGTAAGCGAAGAGGGGTGCAGTGCACAAGGTGTCCCGAAAGAGGTAAGCGAAATAGGTATTGTCTCCCTTGCATACGGAGATGGTATCCCAACACCTCACAGGAAGCCATAGCTGAGGCTTGTCCTGTGTGCCGCGGCAACTGCAACTGCACAGCCTGCCTTCGGTTGGATGCGCGTTTCAGGCGTTTCGAGAATCGAGACCCGAAAATCGATGAGGAGACCAAGCTGGAGCACTCTAAGTATTTGGTTCATCAGCTGCTCCCGTATTTGAAGATGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTGGAAGCCAAGAGGCAGGGGTTGCGTGACCTTTCGGAGCTCAAGGTGGACAAGGCTAATTGCGGTGACGGAGTGCGTATGTACTGCAACAGCTGCAGCACTTCGATTTTTGATTTTCATAGGACTTGTCCCGGATGTGAGTATGATCTGTGCCTCGGTTGCTGCCGGGAGATTCGAGATGGTAAGTGGAACAAGAGAGCTCAAGCGTCCACATTTGAGTGGAAATGGAGAGCAAGTGAAGATGGAAGCATTCTCTGCCCCCCAACACACATGAAAGGTTGTGGTAAGCATAATTTAGAATTGAGATGCCTTTTTTCTCAGAATCATGTTATGGAATTGGTAGAGAAAGCAGGAGAAATAGATGCAAGTTACAATTTTTTGCCTGATCGTAGTGAAACATGTGCATATAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTTGCTCAAGTAGTGGCAGCAGCAGCAGCAGCTCTAAGTTAAGGAAAGCAGCTTCTAGAGTTGCTTCTGATAGTGACTACTTGTTCTGTCCAAGGGTCGGAGAAATCCAAGATGAGGATTTTGGGCATTTTCAGTGGCATTGGAAGAGGGGGGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCTGTCATGTGGCGGGCATTTAGACAGATAAAGAAAACCAATCATGATAGTGATTTGACAGTCCCCGCCATTGATTGCTGGAATTGGTGTTTTATAGAAATCAACCTCCTCCATTTCTTTAATGGCTATTCCAACGGACGGTCTTATGAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATCATCTACCGAGTTTCACAATCGTCTTCCCCGGCATGGTGAGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATACTCACCCCAACAAGGGAACTCTGAACCTTGCTATCTACTTGCCACCTACCTCAGTAAGGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTACACAGGAGCTTGAACGGGGAGATTCTGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTCAATATTCTGACACATTCAGCTGAAGTGGCCCTTACAACTAAACAACTGGCAACTATAGAGAAGTTGAAGAAAAAGCACAGGGAGCAAGACGAAAGAGAGATATTTGGGAATTGTCACACAAATGCTGGAAATGGAAGTGGGCCTCAGCAAGAATCAGCTGAAGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGATTCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTATGCATGTGCTCTGTAATCCTTTGCAGCATGTTACTCATGCTATACTTGATCAGACTGTTTATCTCACTGTAGAGCACAAGAGGAAGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTTCAAAGACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTTTCAAAGTTGCACTGGAGTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACAAACCACAGAGCCAGTGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTATTAAGGAGATTTCTGGTGAGAATGTGATGGTAAAGAGGGCAAGGGATGTGAAGGCAAAGAGGGCAAGGGCTCCCTTGGACGTGTCGACAATTTGCCATCAATGTCAGAGAAATGTTAAGGGAAGAGTGGTGTGGTGCACAAGGTGTGTGAGGAAGAGGTATTGTATTCCTTGCATACAGAGATGGTATCCCAACACATCAGAGGAAGCCATTGCTAAGGCTTGTCCTGTGTGCCGTGGCAACTGCAACTGCACAGCCTGCCTTCGATTGGATGTGCCTGTCAGGCGTTTGCAGAATCTAGACCCGAAAATCGACAAGGGTACCAAGCTGGAGCACTGTAAGTACTTGGTTCATCAGCTGCTTCCCTATTTGAAGAGGATTAGGGATGAGCAGGTGATTGAGATGCTGATCGAGGCTAAGAGGCAGGGATTACGTGATGTTTCGGAGCTCAAGGTGGAGAAGGCTAATTGCGGTGAAGATGAGCGTATGTACTGCAACAACTGCAGCACTTCTATTTTTGATTTTCATAGGAGTTGTCCTGAGTGTGAGTATGATCTGTGCCTCAGTTGCTGCCGGGAGATTCGAGATGGCAGGTGGAAGAAGAGAGGGATGGAGGAGAATGCTCAAGTGTCCACATTTGAGTGGAGAGCAAGTGAAGATGGAAGCATTCTCTGGACACATATGAAATGCTGTGGTAAGCATAAATTAGAATTGAAATGCAAATTTTCTCAGAATCATGTCATAGAATTGGTAGAGAAAGCAGGAAAAATAGATGAAAGTTACAGTCATTTGCGTGCTAGTAGTGAAACACCTGCACACAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTCAAGTAGCAGCTGCTCTATGTTAAGGAAAGCAGCTTCTAGAGATGGTTCTGATGATAACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAAGATAAGGATTTTGGGCATTTTCAGTGGCATTGGATGAGGGGTGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCTGTCATGTGGCGGGCATTTAGAAAGATAAAGAAGATCAAGCATGATAGTGAATTGACAGTCCACGCCATTGATTGCTGGGATTGGTGTTTTATAGAAATCAACATCCTCCATTTCTTTAGTGGATATTCCAACGGACGGCGTTATAAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATCATCTACTGAGTTTCACAATCGGCTTCCCCGGCATGTTAAGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATACTCACCCCAACAAGGGAACTCTGAACCTTGCTATCTACTTGCCACCTACCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACGGGGAGATTCTGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTTAATATTCTGACGCATACAGCTGAAGTGCCCCTTACAACTGAACAACTGGCAACTATAGAGAAGTTGAAGAAAAAGCACAGGGAGCAAGACGAAAGAGAGATATTTGGGAATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCGCAGCAAGAATCCGCTGAAGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGATTCAGGATGTTCCGCTGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTATGCATGTGCTCTGTAATCCTTTGCAGCGTGTTACTCATGCTATACATGATCAGACTGTTTATCTCACTGTAGAGCACAAGAGGAAGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATGCTGTTTTTATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTGGAGTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACAAACCACAGAGCCAGTGAGGACAGGCTGGAGGTGAAGAAAATGATTGTGCATGCTATTTGTGAAGCTGTGAAGGAGATTTCTGCTGAGAATGTGATGACTGAGGTTGTGAAGCCTAGGGGAGGCGCAAGGGAATTAAGTCAAGGTGTAAGGTCCAAGTCTTACAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1486

Amino Acids

169.75

Weight (kDa)

8.33

Isoelectric Point (pI)

50.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 89 - 155 6.7e-06 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 623 - 717 4e-12 JmjC domain, hydroxylase
zf-4CXXC_R1 PF10497 789 - 849 6.5e-07 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 1310 - 1409 5.1e-13 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3477
AarI CACCTGC 4 cut(s) 577, 2662, 2862, 3100
Acc36I ACCTGC 6 cut(s) 577, 2084, 2662, 2862, 3100, 4160
Acc65I GGTACC 1 cut(s) 2600
AccB1I GGYRCC 3 cut(s) 1859, 2600, 3935
AccB7I CCANNNNNTGG 1 cut(s) 3218
AccI GTMKAC 3 cut(s) 1517, 2366, 3593
AccII CGCG 2 cut(s) 432, 472
AccIII TCCGGA 2 cut(s) 1878, 3954
AclWI GGATC 2 cut(s) 1240, 3316
AcoI YGGCCR 1 cut(s) 50
AcsI RAATTY 9 cut(s) 5, 99, 1487, 1931, 2033, 3002, 3563, 4007, 4109
AcuI CTGAAG 4 cut(s) 1722, 1860, 3798, 3936
AdeI CACNNNGTG 2 cut(s) 327, 2424
AfaI GTAC 4 cut(s) 686, 2602, 2624, 2771
AflIII ACRYGT 2 cut(s) 1001, 2361
AjiI CACGTC 4 cut(s) 265, 1924, 2362, 4000
AjnI CCWGG 2 cut(s) 9, 161
AleI CACNNNNGTG 2 cut(s) 3100, 3876
Alw26I GTCTC 4 cut(s) 365, 489, 509, 1397
Alw44I GTGCAC 3 cut(s) 244, 319, 2416
AlwI GGATC 2 cut(s) 1240, 3316
AlwNI CAGNNNCTG 1 cut(s) 2850
Ama87I CYCGRG 1 cut(s) 36
Aor13HI TCCGGA 2 cut(s) 1878, 3954
ApaLI GTGCAC 3 cut(s) 244, 319, 2416
ApoI RAATTY 9 cut(s) 5, 99, 1487, 1931, 2033, 3002, 3563, 4007, 4109
Asp700I GAANNNNTTC 2 cut(s) 2196, 4272
Asp718I GGTACC 1 cut(s) 2600
AspA2I CCTAGG 1 cut(s) 4403
AspLEI GCGC 3 cut(s) 148, 472, 4415
AspS9I GGNCC 7 cut(s) 33, 1589, 1708, 1820, 3665, 3896, 4440
AsuC2I CCSGG 5 cut(s) 735, 770, 1474, 2855, 3550
AvaI CYCGRG 1 cut(s) 36
AvaII GGWCC 3 cut(s) 1589, 3665, 4440
AvrII CCTAGG 1 cut(s) 4403
BaeGI GKGCMC 4 cut(s) 248, 323, 2420, 3786
BaeI ACNNNNGTAYC 2 cut(s) 2584, 2617
BanI GGYRCC 3 cut(s) 1859, 2600, 3935
BanII GRGCYC 5 cut(s) 256, 645, 805, 2353, 2730
BarI GAAGNNNNNNTAC 4 cut(s) 1638, 1670, 3714, 3746
BbsI GAAGAC 1 cut(s) 1460
BbvCI CCTCAGC 2 cut(s) 411, 1824
BceAI ACGGC 8 cut(s) 33, 73, 1028, 2032, 2499, 3116, 3485, 4108
BciT130I CCWGG 2 cut(s) 11, 163
BciVI GTATCC 2 cut(s) 393, 2478
BclI TGATCA 2 cut(s) 1984, 4060
BcnI CCSGG 5 cut(s) 735, 770, 1474, 2855, 3550
BcoDI GTCTC 4 cut(s) 365, 489, 509, 1397
BfaI CTAG 5 cut(s) 1101, 2579, 3078, 3177, 4404
BfmI CTRYAG 8 cut(s) 697, 1734, 2004, 2091, 2782, 3810, 4080, 4167
BfoI RGCGCY 1 cut(s) 149
BfuAI ACCTGC 6 cut(s) 577, 2084, 2662, 2862, 3100, 4160
BfuI GTATCC 2 cut(s) 393, 2478
BglI GCCNNNNNGGC 2 cut(s) 20, 2563
BlnI CCTAGG 1 cut(s) 4403
BmcAI AGTACT 1 cut(s) 2624
Bme1390I CCNGG 7 cut(s) 11, 163, 735, 770, 1474, 2855, 3550
Bme18I GGWCC 3 cut(s) 1589, 3665, 4440
BmeT110I CYCGRG 1 cut(s) 36
BmgBI CACGTC 4 cut(s) 265, 1924, 2362, 4000
BmgT120I GGNCC 7 cut(s) 33, 1589, 1708, 1820, 3665, 3896, 4440
BmrFI CCNGG 7 cut(s) 11, 163, 735, 770, 1474, 2855, 3550
BoxI GACNNNNGTC 3 cut(s) 1587, 3663, 4133
BpiI GAAGAC 1 cut(s) 1460
BpmI CTGGAG 8 cut(s) 545, 1922, 2165, 2261, 2630, 3998, 4241, 4337
Bpu10I CCTNAGC 3 cut(s) 411, 1824, 2496
BpuMI CCSGG 5 cut(s) 735, 770, 1474, 2855, 3550
Bsa29I ATCGAT 1 cut(s) 507
BsaAI YACGTR 1 cut(s) 2714
BsaBI GATNNNNATC 1 cut(s) 2687
BsaI GGTCTC 2 cut(s) 489, 509
BsaWI WCCGGW 2 cut(s) 1878, 3954
Bse1I ACTGG 6 cut(s) 1731, 2148, 2226, 3807, 4224, 4302
Bse3DI GCAATG 3 cut(s) 1222, 2490, 3298
Bse8I GATNNNNATC 1 cut(s) 2687
BseAI TCCGGA 2 cut(s) 1878, 3954
BseBI CCWGG 2 cut(s) 11, 163
BseCI ATCGAT 1 cut(s) 507
BseJI GATNNNNATC 1 cut(s) 2687
BseMI GCAATG 3 cut(s) 1222, 2490, 3298
BseNI ACTGG 6 cut(s) 1731, 2148, 2226, 3807, 4224, 4302
BseRI GAGGAG 3 cut(s) 527, 1352, 2909
BseSI GKGCMC 4 cut(s) 248, 323, 2420, 3786
BseX3I CGGCCG 1 cut(s) 50
BseYI CCCAGC 3 cut(s) 1245, 3321, 3408
BsgI GTGCAG 4 cut(s) 335, 430, 2515, 3078
Bsh1236I CGCG 2 cut(s) 432, 472
Bsh1285I CGRYCG 1 cut(s) 53
BshNI GGYRCC 3 cut(s) 1859, 2600, 3935
BshVI ATCGAT 1 cut(s) 507
BsiEI CGRYCG 1 cut(s) 53
BsiHKCI CYCGRG 1 cut(s) 36
BsiSI CCGG 9 cut(s) 735, 769, 1474, 1580, 1879, 2854, 3550, 3656, 3955
BslFI GGGAC 5 cut(s) 315, 717, 1301, 1883, 3959
BsmAI GTCTC 4 cut(s) 365, 489, 509, 1397
BsmFI GGGAC 5 cut(s) 315, 717, 1301, 1883, 3959
BsmI GAATGC 3 cut(s) 852, 2905, 2946
Bso31I GGTCTC 2 cut(s) 489, 509
BsoBI CYCGRG 1 cut(s) 36
Bsp13I TCCGGA 2 cut(s) 1878, 3954
BspDI ATCGAT 1 cut(s) 507
BspEI TCCGGA 2 cut(s) 1878, 3954
BspFNI CGCG 2 cut(s) 432, 472
BspHI TCATGA 1 cut(s) 1297
BspMAI CTGCAG 4 cut(s) 701, 2095, 2786, 4171
BspMI ACCTGC 6 cut(s) 577, 2084, 2662, 2862, 3100, 4160
BspPI GGATC 2 cut(s) 1240, 3316
BspT107I GGYRCC 3 cut(s) 1859, 2600, 3935
BspTNI GGTCTC 2 cut(s) 489, 509
BsrDI GCAATG 3 cut(s) 1222, 2490, 3298
BsrI ACTGG 6 cut(s) 1731, 2148, 2226, 3807, 4224, 4302
BssNAI GTATAC 2 cut(s) 1518, 3594
BssT1I CCWWGG 5 cut(s) 258, 1136, 2355, 3212, 4403
Bst1107I GTATAC 2 cut(s) 1518, 3594
Bst2UI CCWGG 2 cut(s) 11, 163
Bst4CI ACNGT 9 cut(s) 1315, 1395, 1993, 2005, 2618, 3065, 3391, 4069, 4081
Bst6I CTCTTC 8 cut(s) 301, 1184, 2187, 2398, 2429, 2648, 2876, 4263
BstAPI GCANNNNNTGC 6 cut(s) 453, 625, 696, 858, 2538, 2781
BstBAI YACGTR 1 cut(s) 2714
BstC8I GCNNGC 9 cut(s) 81, 141, 454, 1269, 2265, 2539, 3075, 3345, 4341
BstDSI CCRYGG 5 cut(s) 15, 430, 2048, 2515, 4124
BstENI CCTNNNNNAGG 3 cut(s) 166, 1573, 4407
BstFNI CGCG 2 cut(s) 432, 472
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 3 cut(s) 148, 472, 4415
BstMAI GTCTC 4 cut(s) 365, 489, 509, 1397
BstMCI CGRYCG 1 cut(s) 53
BstNI CCWGG 2 cut(s) 11, 163
BstNSI RCATGY 7 cut(s) 1005, 1943, 1967, 2267, 3557, 4019, 4343
BstPAI GACNNNNGTC 3 cut(s) 1587, 3663, 4133
BstSCI CCNGG 7 cut(s) 9, 161, 733, 768, 1472, 2853, 3548
BstSFI CTRYAG 8 cut(s) 697, 1734, 2004, 2091, 2782, 3810, 4080, 4167
BstSLI GKGCMC 4 cut(s) 248, 323, 2420, 3786
BstUI CGCG 2 cut(s) 432, 472
BstV2I GAAGAC 1 cut(s) 1460
BstXI CCANNNNNNTGG 1 cut(s) 4145
BstZ17I GTATAC 2 cut(s) 1518, 3594
BstZI CGGCCG 1 cut(s) 50
Bsu15I ATCGAT 1 cut(s) 507
BsuI GTATCC 2 cut(s) 393, 2478
BsuTUI ATCGAT 1 cut(s) 507
BtgI CCRYGG 5 cut(s) 15, 430, 2048, 2515, 4124
BtrI CACGTC 4 cut(s) 265, 1924, 2362, 4000
BtsI GCAGTG 3 cut(s) 323, 1657, 3733
BveI ACCTGC 6 cut(s) 577, 2084, 2662, 2862, 3100, 4160
Cac8I GCNNGC 9 cut(s) 81, 141, 454, 1269, 2265, 2539, 3075, 3345, 4341
CaiI CAGNNNCTG 1 cut(s) 2850
CciI TCATGA 1 cut(s) 1297
CfoI GCGC 3 cut(s) 148, 472, 4415
Cfr13I GGNCC 7 cut(s) 33, 1589, 1708, 1820, 3665, 3896, 4440
Cfr42I CCGCGG 1 cut(s) 433
ClaI ATCGAT 1 cut(s) 507
CseI GACGC 2 cut(s) 798, 3775
Csp6I GTAC 4 cut(s) 685, 2601, 2623, 2770
CviQI GTAC 4 cut(s) 685, 2601, 2623, 2770
DraIII CACNNNGTG 2 cut(s) 327, 2424
EaeI YGGCCR 1 cut(s) 50
EagI CGGCCG 1 cut(s) 50
Eam1104I CTCTTC 8 cut(s) 301, 1184, 2187, 2398, 2429, 2648, 2876, 4263
EarI CTCTTC 8 cut(s) 301, 1184, 2187, 2398, 2429, 2648, 2876, 4263
EciI GGCGGA 1 cut(s) 193
Ecl136II GAGCTC 3 cut(s) 643, 803, 2728
EclXI CGGCCG 1 cut(s) 50
Eco130I CCWWGG 5 cut(s) 258, 1136, 2355, 3212, 4403
Eco24I GRGCYC 5 cut(s) 256, 645, 805, 2353, 2730
Eco31I GGTCTC 2 cut(s) 489, 509
Eco47I GGWCC 3 cut(s) 1589, 3665, 4440
Eco52I CGGCCG 1 cut(s) 50
Eco53kI GAGCTC 3 cut(s) 643, 803, 2728
Eco57I CTGAAG 4 cut(s) 1722, 1860, 3798, 3936
Eco88I CYCGRG 1 cut(s) 36
EcoICRI GAGCTC 3 cut(s) 643, 803, 2728
EcoNI CCTNNNNNAGG 3 cut(s) 166, 1573, 4407
EcoO109I RGGNCCY 1 cut(s) 33
EcoRI GAATTC 4 cut(s) 5, 99, 2033, 4109
EcoRII CCWGG 2 cut(s) 9, 161
EcoT14I CCWWGG 5 cut(s) 258, 1136, 2355, 3212, 4403
EcoT22I ATGCAT 2 cut(s) 1941, 4017
EcoT38I GRGCYC 5 cut(s) 256, 645, 805, 2353, 2730
ErhI CCWWGG 5 cut(s) 258, 1136, 2355, 3212, 4403
FalI AAGNNNNNCTT 4 cut(s) 1695, 1727, 3771, 3803
FaqI GGGAC 5 cut(s) 315, 717, 1301, 1883, 3959
FauI CCCGC 3 cut(s) 1260, 1327, 3336
FauNDI CATATG 1 cut(s) 2961
FbaI TGATCA 2 cut(s) 1984, 4060
FblI GTMKAC 3 cut(s) 1517, 2366, 3593
FriOI GRGCYC 5 cut(s) 256, 645, 805, 2353, 2730
FspBI CTAG 5 cut(s) 1101, 2579, 3078, 3177, 4404
GlaI GCGC 3 cut(s) 147, 471, 4414
GsaI CCCAGC 3 cut(s) 1249, 3325, 3412
GsuI CTGGAG 8 cut(s) 545, 1922, 2165, 2261, 2630, 3998, 4241, 4337
HaeII RGCGCY 1 cut(s) 149
HapII CCGG 9 cut(s) 735, 769, 1474, 1580, 1879, 2854, 3550, 3656, 3955
HgaI GACGC 2 cut(s) 798, 3775
HhaI GCGC 3 cut(s) 148, 472, 4415
Hin6I GCGC 3 cut(s) 146, 470, 4413
HinP1I GCGC 3 cut(s) 146, 470, 4413
HincII GTYRAC 1 cut(s) 2367
HindII GTYRAC 1 cut(s) 2367
HindIII AAGCTT 3 cut(s) 152, 1652, 3728
HpaII CCGG 9 cut(s) 735, 769, 1474, 1580, 1879, 2854, 3550, 3656, 3955
Hpy99I CGWCG 2 cut(s) 113, 116
HpyAV CCTTC 6 cut(s) 212, 467, 2326, 2552, 2734, 4354
HpyCH4III ACNGT 9 cut(s) 1315, 1395, 1993, 2005, 2618, 3065, 3391, 4069, 4081
HpyCH4IV ACGT 5 cut(s) 264, 1923, 2361, 2713, 3999
HpySE526I ACGT 5 cut(s) 264, 1923, 2361, 2713, 3999
HspAI GCGC 3 cut(s) 146, 470, 4413
Kpn2I TCCGGA 2 cut(s) 1878, 3954
KpnI GGTACC 1 cut(s) 2604
Ksp22I TGATCA 2 cut(s) 1984, 4060
KspI CCGCGG 1 cut(s) 433
MaeI CTAG 5 cut(s) 1101, 2579, 3078, 3177, 4404
MaeII ACGT 5 cut(s) 264, 1923, 2361, 2713, 3999
MfeI CAATTG 1 cut(s) 120
MmeI TCCRAC 7 cut(s) 444, 1123, 1410, 2234, 3199, 3486, 4310
Mph1103I ATGCAT 2 cut(s) 1941, 4017
MroI TCCGGA 2 cut(s) 1878, 3954
MroXI GAANNNNTTC 2 cut(s) 2196, 4272
MspI CCGG 9 cut(s) 735, 769, 1474, 1580, 1879, 2854, 3550, 3656, 3955
MspR9I CCNGG 7 cut(s) 11, 163, 735, 770, 1474, 2855, 3550
MunI CAATTG 1 cut(s) 120
Mva1269I GAATGC 3 cut(s) 852, 2905, 2946
MvaI CCWGG 2 cut(s) 11, 163
MvnI CGCG 2 cut(s) 432, 472
NciI CCSGG 5 cut(s) 735, 770, 1474, 2855, 3550
NdeI CATATG 1 cut(s) 2961
NmeAIII GCCGAG 1 cut(s) 109
NmuCI GTSAC 5 cut(s) 629, 668, 1118, 1793, 3869
NsiI ATGCAT 2 cut(s) 1941, 4017
NspI RCATGY 7 cut(s) 1005, 1943, 1967, 2267, 3557, 4019, 4343
OliI CACNNNNGTG 2 cut(s) 3100, 3876
PaeI GCATGC 2 cut(s) 2267, 4343
PagI TCATGA 1 cut(s) 1297
PaqCI CACCTGC 4 cut(s) 577, 2662, 2862, 3100
PasI CCCWGGG 1 cut(s) 162
PciI ACATGT 1 cut(s) 1001
PctI GAATGC 3 cut(s) 852, 2905, 2946
PdmI GAANNNNTTC 2 cut(s) 2196, 4272
PflMI CCANNNNNTGG 1 cut(s) 3218
PfoI TCCNGGA 1 cut(s) 733
Ppu21I YACGTR 1 cut(s) 2714
PscI ACATGT 1 cut(s) 1001
PshAI GACNNNNGTC 3 cut(s) 1587, 3663, 4133
PsiI TTATAA 1 cut(s) 3477
Psp124BI GAGCTC 3 cut(s) 645, 805, 2730
Psp6I CCWGG 2 cut(s) 9, 161
PspFI CCCAGC 3 cut(s) 1245, 3321, 3408
PspGI CCWGG 2 cut(s) 9, 161
PspPI GGNCC 7 cut(s) 33, 1589, 1708, 1820, 3665, 3896, 4440
PstI CTGCAG 4 cut(s) 701, 2095, 2786, 4171
PstNI CAGNNNCTG 1 cut(s) 2850
PvuII CAGCTG 8 cut(s) 553, 696, 1700, 1838, 1900, 2638, 3150, 3776
RsaI GTAC 4 cut(s) 686, 2602, 2624, 2771
RsaNI GTAC 4 cut(s) 685, 2601, 2623, 2770
SacI GAGCTC 3 cut(s) 645, 805, 2730
SacII CCGCGG 1 cut(s) 433
SalI GTCGAC 1 cut(s) 2365
Sau96I GGNCC 7 cut(s) 33, 1589, 1708, 1820, 3665, 3896, 4440
SbfI CCTGCAGG 2 cut(s) 2095, 4171
ScaI AGTACT 1 cut(s) 2624
ScrFI CCNGG 7 cut(s) 11, 163, 735, 770, 1474, 2855, 3550
SdaI CCTGCAGG 2 cut(s) 2095, 4171
SfcI CTRYAG 8 cut(s) 697, 1734, 2004, 2091, 2782, 3810, 4080, 4167
Sfr303I CCGCGG 1 cut(s) 433
SgrBI CCGCGG 1 cut(s) 433
SinI GGWCC 3 cut(s) 1589, 3665, 4440
SphI GCATGC 2 cut(s) 2267, 4343
Sse8387I CCTGCAGG 2 cut(s) 2095, 4171
SspI AATATT 2 cut(s) 1684, 3760
SspMI CTAG 5 cut(s) 1101, 2579, 3078, 3177, 4404
SstI GAGCTC 3 cut(s) 645, 805, 2730
StyD4I CCNGG 7 cut(s) 9, 161, 733, 768, 1472, 2853, 3548
StyI CCWWGG 5 cut(s) 258, 1136, 2355, 3212, 4403
TaaI ACNGT 9 cut(s) 1315, 1395, 1993, 2005, 2618, 3065, 3391, 4069, 4081
TaiI ACGT 5 cut(s) 267, 1926, 2364, 2716, 4002
TatI WGTACW 3 cut(s) 684, 2622, 2769
TauI GCSGC 7 cut(s) 23, 52, 178, 236, 432, 435, 3901
TseFI GTSAC 5 cut(s) 629, 668, 1118, 1793, 3869
Tsp45I GTSAC 5 cut(s) 629, 668, 1118, 1793, 3869
TspGWI ACGGA 7 cut(s) 129, 388, 687, 1404, 2204, 3480, 4280
Van91I CCANNNNNTGG 1 cut(s) 3218
VneI GTGCAC 3 cut(s) 244, 319, 2416
VpaK11BI GGWCC 3 cut(s) 1589, 3665, 4440
XagI CCTNNNNNAGG 3 cut(s) 166, 1573, 4407
XapI RAATTY 9 cut(s) 5, 99, 1487, 1931, 2033, 3002, 3563, 4007, 4109
XbaI TCTAGA 3 cut(s) 1100, 2578, 3176
XceI RCATGY 7 cut(s) 1005, 1943, 1967, 2267, 3557, 4019, 4343
XcmI CCANNNNNNNNNTGG 4 cut(s) 1260, 1373, 3336, 3449
XmaJI CCTAGG 1 cut(s) 4403
XmiI GTMKAC 3 cut(s) 1517, 2366, 3593
XmnI GAANNNNTTC 2 cut(s) 2196, 4272
XspI CTAG 5 cut(s) 1101, 2579, 3078, 3177, 4404
ZrmI AGTACT 1 cut(s) 2624
Zsp2I ATGCAT 2 cut(s) 1941, 4017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.