Rh7CG383400

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000280
Physical Location & Seq
Forward (+)
48312316 .. 48319471
7156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG383400.1

Sequence Viewer

Length: 4698 bp
ATGAGAATCCAGGCGACGCCGTCGAGATTGTGGCCCCGAGGCACAAAGCGGCCGAACATATGGGGAATTCAGAACGACGACGGAACAAATGGGGAAGATGGTGGAGGATCGTCTGTGGAGCCACTGCCAAAGCTTGTTCCCTGGGGGAGGAAGCGACGGAGGCTAGGATGCGAGGGTTTTTGGGCAAATCAAAATGAAGGAAAGGCAAGGGCTGTGAAGGCGAAGAAGAAGAAGAAGAAGCAGCAGCAGCAGAGAAGGGCTCCCTTGGAGGTGCAGCAGCAGACAAGGGATAAGCAAGTACCACTACCTAGCTACCCGCAGAGAAGGGCTCCCTTGGAGGTGCAGCAGCAGAGTAGGGATAAACAAGTACCACTACCTAGCTACCTGCAGAGAAGGGCTCCCTTGAAGGTACGGACAATCAAGTGCCATCAATGTAAGCAAAATGATATGGGAAGAGCGGTGCGGTGCACAAAGTGTTCGAGGAAGAGGTATTGTATCCCTTGCATAAGGACTTGGTATCCCAACACCTCAGAGGAAGCCATAGCTGAGGCTTGTCCTGCGTGCCGCGGCAACTGCAACTGCAATGTCTGCCTTTGTTGGGGTGTGCCTGTCAGGAATCTGAACCCGAAAATCGACAAGGAGGCCAAGCTGGATCACTCTAAGTATTTGGTTCATCAGCTGCTTCCGTATTTGAAGATGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTGGAAGCCAAGAGGCAGGGCTTACGTGACCTTTCGGAGCTCAAGGTGGACAAGGCTAAAAGCGGTGGTGACCAAGTGCGTATGTACTGCAACAGCTGCAGCACTTCGATTTTTGATTTTCATAGGACTTGTCCCGGGTGTGAGTATGATCTGTGCCTCGGCTGCTGCCGGGAGATTCGAGATGGCAAGTGGAACAAGAGAGCTCAAGCGTCCACATTTGAGTGGAAATGGATAGCAAATGACGGCGGAAACATTTTCTGCCCACCAACACACATGAAAGGTTGTGGTAAGCATAATTTAGAATTGAGATGCATTTTTCCTCAGAATCATGTCATGGAGTTGGTAGAGAAAGCAGAAGAAATAGATGCAAGTTACAATTTTTTGCCTGATTATAGTGAAACATGTGCATATAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTCAAGTAGCAGCAGTTGTAAGTTAAGGAAAGCAGCTTCTAGAGTTGCTTCTGATAGTGACTACTTGTTCTGTCCAAGGGTTGGACAAATTCAAGATAAGGATTTTGGGCATTTTCAGTGGCATTGGAAGAGGGGGGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCTGTCATGTGGCGGGCATTTAGACAGATAGAGAAAGTCAAGAATGATAGTGAATTGACATTCCACGCCATTGATTGTTGGGATTGGTGTTCTATAGAAATCAACATCCTCCATTTCTTTAGTGGATATTCCAACGGACGGTCTCATAAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATCATCTGCTGAATTTTACAAACGTCTTCCCCGGCATTGTAAGGAATTTTTATGTTGTCTGCCTCTCAAGGAGTATACTCACCCCAACAAGGGAACTTTGAACCTTGCTACCTACTTGCCACCTGCCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACAGGGAGACTCCGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTTAATATTCTGACACATACAGCTGAAGTGGCTCTTACAACTGAACAACTTGCAACTATAGAGAAGTTGAAGAAAAAGCACAGGGAGCAAAACGAAAGAGAGATATTTGGGGATTGTCACCCAAGTGCGGGAAATGGAAGTGGGCCTCAGCAAGAGTCAGCTATAGAGAAGTTGAAGAGAAAGTATAGGGAGCAAAACGAAAGAGAGATTTTTGGGAATTGTCACACAAGTGCTGGAAGTGGAAGTGGGCCTCAGGATGAGTCAGCTGAAGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGATCCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTATGCATGTACATGGTAATCCTTTGCAGCATGTTACTCATGCTATACATGATCAGACTGTCTATCTCACTGTGGAACACAAGAGGAAGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTTGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGATGGAAGAGTTTCGCACACTTCCGACAAACCACAGAGCCCATGAGGATAAGCTGGAGGTGAAGAAAATGATTGTGCATGCAGTTTGTGAAGCTGTGAAGGAGATTTCTGGTGAGAATGTGACAAAGAGGGTAAGGGCTGTGAAGGCAAAGAGGGCAAGGGACGAGTCGATACACTGCCATCAGTGTCATAGATATGTTATGGGAAGAGTAGTGCAGTGCACAAGGTGTCTGGAAAGAGGTATGGGAATGAGGACGAGGTATTGTATCCCTTGTATACAGAGATGGTATCCCAACACATCAGAGGAAGCCATTGCTAAGGCTTGTCCTGTATGCCACGGCAACTGCAACTGCACAGCCTGCCTTCGTTTGGACGTGCCTGTACGGTGTTTGCAGAATCTAGACCCCAAAATCGACAGGGGTACCAAGCTGGAGCACTGTAAGTATTTGGTTCATCAGCTGCTTCCCTATTTGAAGAGGATTAGGGATCAGCAGGTGAGTGAGATGGTGGTCGAGGCTAAGAGGCGGGGATTACGTGATGTTTTGGAGCTCAAGGTGGAGAAGGCTAAGTGCGGTGAAGATGAGCGTATGTACTGCAACAACTGCAGCACTTCTATTTTTGATTTTCATAGGAGTTGTCCCAAGTGTGGGTATGATCTGTGCCTCAGTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAAGAAGAGAGAGGTGGAGGAGGAAGCTCAAGTGTCCACATTTGAGTGGAGATTTAGTGAAGATGGAAGCATTCTCTGCCCCCCAACACAAAAGAAATGTTTTGGTAAGCATAATTTAGAATTGAGATGCATTTTTTCTCAGAATCATGTCATGGAATTGGTAGAGAAAGCAGGAAAAATAGATGCGAGTTACAATTTTTTGCCTGATCGTAGTGAAACATGTGCATACAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTCAAGCAGCAGCAGCAGCTGTAAGTTAAGGAAAGCAGCTTCTAGAGTATCTTCTGATAGTAACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAAGATAAGGATTTTGGGCATTTTCAGTGGCATTGGATGAGGGGCGAGCCTGTGGTCGTTAGCAATGCATTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCCGTCATGTGGCGGGCACTTAGAAAGACAGTTAATAACTGCCATGCTAGTGAATTGACATTCCACGCCGTTGATTGCTGGGATTGGTGTTTTATAAGAATCAAAATCCGCCATTTCTTTAGTGGATATTCCAAGGGACGGTCTTATAAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATTATCTACTGAGTTTCACAATCGTCTTCCCCGCCATGGTAGGGAATTTTTATGTTGTCTGCCTTTCATGGAGTATAGTCACCCCAACAAGGGAACTCTGAACCTTGCTACCTACTTGCCACCTACCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACAGGGAGATTCTGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTTAATATTCTGACACATACAGCGGAAGTGGCCCTTACAACTGAACAACTTGCAACTATAGACAAGTTGAAGAAAAAGCACAGGGAGCAAGACAAAAGAGAGATATTTGGGAATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCTCTGCAAGAATTAGCTGAAGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGACTCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCATGTCAATGAATTTATGCATGTGCTCTGTAATCCTTTGCAGCATGTTACTCATGCTATACATGATCAGACTGTTTATCTCACTGTGGAGCACAAGAGGAGGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAGCTTGGAGATGCTGTTTTTATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTGTCAAAGTTGCACTGGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACTGAAGAGTTTCGCACACTTCCGACAAACCACAGAGCCCATGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTGTGAAGGAGATTTCTGGTGAGAATGTGATGACTGAGGATGCGAAGTCTAGGGGAGGCGCAAGGAAATTAAGGCAAGGTGTAAGGCGCATGTCTTACAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1565

Amino Acids

178.97

Weight (kDa)

8.77

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 139 - 198 6.3e-07 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 695 - 791 4.6e-13 JmjC domain, hydroxylase
zf-4CXXC_R1 PF10497 858 - 925 4.6e-06 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 1392 - 1488 3.9e-13 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3662, 3714
AarI CACCTGC 3 cut(s) 703, 1690, 2890
Acc36I ACCTGC 6 cut(s) 393, 703, 1690, 2306, 2890, 4397
Acc65I GGTACC 1 cut(s) 2828
AccB1I GGYRCC 3 cut(s) 2081, 2828, 4172
AccB7I CCANNNNNTGG 2 cut(s) 1259, 3455
AccBSI CCGCTC 1 cut(s) 458
AccI GTMKAC 2 cut(s) 1634, 2683
AccII CGCG 1 cut(s) 567
AccIII TCCGGA 2 cut(s) 2100, 4191
AclWI GGATC 7 cut(s) 115, 660, 1357, 2098, 2111, 2901, 3553
AcoI YGGCCR 1 cut(s) 50
AcsI RAATTY 9 cut(s) 66, 1266, 1571, 1604, 2153, 2255, 3800, 4244, 4346
AcuI CTGAAG 4 cut(s) 1839, 2082, 4173, 4524
AcyI GRCGYC 1 cut(s) 17
AdeI CACNNNGTG 2 cut(s) 474, 2634
AfaI GTAC 8 cut(s) 300, 369, 411, 815, 2166, 2790, 2830, 2999
AflIII ACRYGT 2 cut(s) 1130, 3323
AjiI CACGTC 2 cut(s) 2146, 2782
AjnI CCWGG 3 cut(s) 9, 140, 2106
AleI CACNNNNGTG 3 cut(s) 1917, 2022, 4113
Alw21I GWGCWC 8 cut(s) 470, 771, 934, 2630, 2844, 2958, 4260, 4326
Alw26I GTCTC 2 cut(s) 1518, 1749
Alw44I GTGCAC 2 cut(s) 466, 2626
AlwI GGATC 7 cut(s) 115, 660, 1357, 2098, 2111, 2901, 3553
AlwNI CAGNNNCTG 2 cut(s) 3078, 3390
Ama87I CYCGRG 2 cut(s) 36, 863
Aor13HI TCCGGA 2 cut(s) 2100, 4191
AoxI GGCC 9 cut(s) 32, 50, 642, 1529, 1937, 2042, 3725, 4020, 4133
ApaLI GTGCAC 2 cut(s) 466, 2626
ApoI RAATTY 9 cut(s) 66, 1266, 1571, 1604, 2153, 2255, 3800, 4244, 4346
Asp700I GAANNNNTTC 2 cut(s) 2418, 4509
Asp718I GGTACC 1 cut(s) 2828
AspLEI GCGC 2 cut(s) 4652, 4680
AspS9I GGNCC 7 cut(s) 33, 1706, 1937, 2042, 3902, 4021, 4133
AsuC2I CCSGG 5 cut(s) 864, 865, 899, 1591, 3083
AvaI CYCGRG 2 cut(s) 36, 863
AvaII GGWCC 2 cut(s) 1706, 3902
AxyI CCTNAGG 1 cut(s) 2046
BaeGI GKGCMC 3 cut(s) 470, 2630, 3586
BaeI ACNNNNGTAYC 2 cut(s) 2812, 2845
BamHI GGATCC 1 cut(s) 2103
BanI GGYRCC 3 cut(s) 2081, 2828, 4172
BanII GRGCYC 8 cut(s) 262, 331, 400, 771, 934, 2449, 2958, 4540
BarI GAAGNNNNNNTAC 4 cut(s) 1755, 1787, 3951, 3983
BbsI GAAGAC 2 cut(s) 1577, 3773
Bbv12I GWGCWC 8 cut(s) 470, 771, 934, 2630, 2844, 2958, 4260, 4326
BbvCI CCTCAGC 2 cut(s) 546, 1941
BceAI ACGGC 8 cut(s) 4, 988, 1157, 2254, 2761, 3350, 3620, 4345
BciT130I CCWGG 3 cut(s) 11, 142, 2108
BciVI GTATCC 4 cut(s) 506, 528, 2684, 2706
BclI TGATCA 2 cut(s) 2206, 4297
BcnI CCSGG 5 cut(s) 864, 865, 899, 1591, 3083
BcoDI GTCTC 2 cut(s) 1518, 1749
BfaI CTAG 8 cut(s) 164, 309, 378, 1218, 2807, 3414, 3615, 4641
BfmI CTRYAG 9 cut(s) 386, 826, 1464, 1851, 1956, 2313, 3010, 4047, 4404
BfuAI ACCTGC 6 cut(s) 393, 703, 1690, 2306, 2890, 4397
BfuI GTATCC 4 cut(s) 506, 528, 2684, 2706
Bme1390I CCNGG 8 cut(s) 11, 142, 864, 865, 899, 1591, 2108, 3083
Bme18I GGWCC 2 cut(s) 1706, 3902
BmeT110I CYCGRG 2 cut(s) 36, 863
BmgBI CACGTC 2 cut(s) 2146, 2782
BmgT120I GGNCC 7 cut(s) 33, 1706, 1937, 2042, 3902, 4021, 4133
BmrFI CCNGG 8 cut(s) 11, 142, 864, 865, 899, 1591, 2108, 3083
BoxI GACNNNNGTC 4 cut(s) 1704, 2279, 3900, 4370
BpiI GAAGAC 2 cut(s) 1577, 3773
BplI GAGNNNNNCTC 6 cut(s) 244, 276, 313, 345, 382, 414
BpmI CTGGAG 5 cut(s) 2144, 2483, 2858, 4235, 4574
Bpu10I CCTNAGC 3 cut(s) 546, 1941, 2724
BpuMI CCSGG 5 cut(s) 864, 865, 899, 1591, 3083
BsaAI YACGTR 2 cut(s) 755, 2942
BsaHI GRCGYC 1 cut(s) 17
BsaI GGTCTC 1 cut(s) 1518
BsaWI WCCGGW 2 cut(s) 2100, 4191
Bse1I ACTGG 1 cut(s) 4461
Bse21I CCTNAGG 1 cut(s) 2046
Bse3DI GCAATG 4 cut(s) 589, 1339, 2718, 3535
BseAI TCCGGA 2 cut(s) 2100, 4191
BseBI CCWGG 3 cut(s) 11, 142, 2108
BseMI GCAATG 4 cut(s) 589, 1339, 2718, 3535
BseNI ACTGG 1 cut(s) 4461
BseRI GAGGAG 2 cut(s) 3137, 4345
BseSI GKGCMC 3 cut(s) 470, 2630, 3586
BseX3I CGGCCG 1 cut(s) 50
BseYI CCCAGC 3 cut(s) 1362, 3558, 3645
BsgI GTGCAG 4 cut(s) 293, 362, 2642, 2743
Bsh1236I CGCG 1 cut(s) 567
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 9 cut(s) 34, 52, 644, 1531, 1939, 2044, 3727, 4022, 4135
BshNI GGYRCC 3 cut(s) 2081, 2828, 4172
BsiEI CGRYCG 1 cut(s) 53
BsiHKAI GWGCWC 8 cut(s) 470, 771, 934, 2630, 2844, 2958, 4260, 4326
BsiHKCI CYCGRG 2 cut(s) 36, 863
BsiSI CCGG 8 cut(s) 864, 898, 1591, 1697, 2101, 3082, 3893, 4192
BslFI GGGAC 6 cut(s) 846, 2105, 2582, 3030, 3717, 4196
BsmAI GTCTC 2 cut(s) 1518, 1749
BsmFI GGGAC 6 cut(s) 846, 2105, 2582, 3030, 3717, 4196
BsmI GAATGC 1 cut(s) 3174
BsnI GGCC 9 cut(s) 34, 52, 644, 1531, 1939, 2044, 3727, 4022, 4135
Bso31I GGTCTC 1 cut(s) 1518
BsoBI CYCGRG 2 cut(s) 36, 863
Bsp13I TCCGGA 2 cut(s) 2100, 4191
Bsp1407I TGTACA 1 cut(s) 2164
Bsp19I CCATGG 1 cut(s) 3790
BspANI GGCC 9 cut(s) 34, 52, 644, 1531, 1939, 2044, 3727, 4022, 4135
BspEI TCCGGA 2 cut(s) 2100, 4191
BspFNI CGCG 1 cut(s) 567
BspMAI CTGCAG 5 cut(s) 390, 830, 2317, 3014, 4408
BspMI ACCTGC 6 cut(s) 393, 703, 1690, 2306, 2890, 4397
BspPI GGATC 7 cut(s) 115, 660, 1357, 2098, 2111, 2901, 3553
BspQI GCTCTTC 1 cut(s) 448
BspT107I GGYRCC 3 cut(s) 2081, 2828, 4172
BspTNI GGTCTC 1 cut(s) 1518
BsrBI CCGCTC 1 cut(s) 458
BsrDI GCAATG 4 cut(s) 589, 1339, 2718, 3535
BsrGI TGTACA 1 cut(s) 2164
BsrI ACTGG 1 cut(s) 4461
BssNAI GTATAC 2 cut(s) 1635, 2684
BssNI GRCGYC 1 cut(s) 17
BssT1I CCWWGG 6 cut(s) 264, 333, 1253, 3449, 3699, 3790
Bst1107I GTATAC 2 cut(s) 1635, 2684
Bst2UI CCWGG 3 cut(s) 11, 142, 2108
Bst4CI ACNGT 9 cut(s) 1512, 2215, 2227, 2793, 2846, 3598, 3708, 4306, 4318
Bst6I CTCTTC 9 cut(s) 448, 479, 1301, 1964, 2409, 2608, 2876, 3104, 4500
BstACI GRCGYC 1 cut(s) 17
BstAPI GCANNNNNTGC 5 cut(s) 588, 825, 2766, 3009, 3180
BstAUI TGTACA 1 cut(s) 2164
BstBAI YACGTR 2 cut(s) 755, 2942
BstC8I GCNNGC 7 cut(s) 562, 1386, 2487, 2767, 3512, 3582, 4578
BstDSI CCRYGG 5 cut(s) 565, 2270, 2743, 3790, 4361
BstEII GGTNACC 1 cut(s) 797
BstENI CCTNNNNNAGG 1 cut(s) 145
BstFNI CGCG 1 cut(s) 567
BstHHI GCGC 2 cut(s) 4652, 4680
BstMAI GTCTC 2 cut(s) 1518, 1749
BstMCI CGRYCG 1 cut(s) 53
BstNI CCWGG 3 cut(s) 11, 142, 2108
BstPAI GACNNNNGTC 4 cut(s) 1704, 2279, 3900, 4370
BstPI GGTNACC 1 cut(s) 797
BstSCI CCNGG 8 cut(s) 9, 140, 862, 863, 897, 1589, 2106, 3081
BstSFI CTRYAG 9 cut(s) 386, 826, 1464, 1851, 1956, 2313, 3010, 4047, 4404
BstSLI GKGCMC 3 cut(s) 470, 2630, 3586
BstUI CGCG 1 cut(s) 567
BstV2I GAAGAC 2 cut(s) 1577, 3773
BstX2I RGATCY 1 cut(s) 2103
BstXI CCANNNNNNTGG 2 cut(s) 2291, 4382
BstYI RGATCY 1 cut(s) 2103
BstZ17I GTATAC 2 cut(s) 1635, 2684
BstZI CGGCCG 1 cut(s) 50
Bsu36I CCTNAGG 1 cut(s) 2046
BsuI GTATCC 4 cut(s) 506, 528, 2684, 2706
BsuRI GGCC 9 cut(s) 34, 52, 644, 1531, 1939, 2044, 3727, 4022, 4135
BtgI CCRYGG 5 cut(s) 565, 2270, 2743, 3790, 4361
BtrI CACGTC 2 cut(s) 2146, 2782
BtsI GCAGTG 5 cut(s) 122, 1774, 2581, 2630, 3970
BveI ACCTGC 6 cut(s) 393, 703, 1690, 2306, 2890, 4397
Cac8I GCNNGC 7 cut(s) 562, 1386, 2487, 2767, 3512, 3582, 4578
CaiI CAGNNNCTG 2 cut(s) 3078, 3390
CfoI GCGC 2 cut(s) 4652, 4680
Cfr13I GGNCC 7 cut(s) 33, 1706, 1937, 2042, 3902, 4021, 4133
Cfr42I CCGCGG 1 cut(s) 568
Cfr9I CCCGGG 1 cut(s) 863
CseI GACGC 2 cut(s) 25, 927
Csp6I GTAC 8 cut(s) 299, 368, 410, 814, 2165, 2789, 2829, 2998
CviQI GTAC 8 cut(s) 299, 368, 410, 814, 2165, 2789, 2829, 2998
DraIII CACNNNGTG 2 cut(s) 474, 2634
EaeI YGGCCR 1 cut(s) 50
EagI CGGCCG 1 cut(s) 50
Eam1104I CTCTTC 9 cut(s) 448, 479, 1301, 1964, 2409, 2608, 2876, 3104, 4500
EarI CTCTTC 9 cut(s) 448, 479, 1301, 1964, 2409, 2608, 2876, 3104, 4500
EciI GGCGGA 2 cut(s) 990, 3665
Ecl136II GAGCTC 3 cut(s) 769, 932, 2956
EclXI CGGCCG 1 cut(s) 50
Eco130I CCWWGG 6 cut(s) 264, 333, 1253, 3449, 3699, 3790
Eco24I GRGCYC 8 cut(s) 262, 331, 400, 771, 934, 2449, 2958, 4540
Eco31I GGTCTC 1 cut(s) 1518
Eco47I GGWCC 2 cut(s) 1706, 3902
Eco52I CGGCCG 1 cut(s) 50
Eco53kI GAGCTC 3 cut(s) 769, 932, 2956
Eco57I CTGAAG 4 cut(s) 1839, 2082, 4173, 4524
Eco81I CCTNAGG 1 cut(s) 2046
Eco88I CYCGRG 2 cut(s) 36, 863
Eco91I GGTNACC 1 cut(s) 797
EcoICRI GAGCTC 3 cut(s) 769, 932, 2956
EcoNI CCTNNNNNAGG 1 cut(s) 145
EcoO65I GGTNACC 1 cut(s) 797
EcoRI GAATTC 3 cut(s) 66, 2255, 4346
EcoRII CCWGG 3 cut(s) 9, 140, 2106
EcoT14I CCWWGG 6 cut(s) 264, 333, 1253, 3449, 3699, 3790
EcoT22I ATGCAT 5 cut(s) 1043, 2163, 3236, 3535, 4254
EcoT38I GRGCYC 8 cut(s) 262, 331, 400, 771, 934, 2449, 2958, 4540
ErhI CCWWGG 6 cut(s) 264, 333, 1253, 3449, 3699, 3790
FalI AAGNNNNNCTT 6 cut(s) 1812, 1844, 2349, 2381, 4008, 4040
FaqI GGGAC 6 cut(s) 846, 2105, 2582, 3030, 3717, 4196
FauI CCCGC 6 cut(s) 324, 1377, 1915, 2925, 3573, 3794
FauNDI CATATG 1 cut(s) 59
FbaI TGATCA 2 cut(s) 2206, 4297
FblI GTMKAC 2 cut(s) 1634, 2683
FriOI GRGCYC 8 cut(s) 262, 331, 400, 771, 934, 2449, 2958, 4540
FspBI CTAG 8 cut(s) 164, 309, 378, 1218, 2807, 3414, 3615, 4641
GlaI GCGC 2 cut(s) 4651, 4679
GsaI CCCAGC 3 cut(s) 1366, 3562, 3649
GsuI CTGGAG 5 cut(s) 2144, 2483, 2858, 4235, 4574
HaeIII GGCC 9 cut(s) 34, 52, 644, 1531, 1939, 2044, 3727, 4022, 4135
HapII CCGG 8 cut(s) 864, 898, 1591, 1697, 2101, 3082, 3893, 4192
HgaI GACGC 2 cut(s) 25, 927
HhaI GCGC 2 cut(s) 4652, 4680
Hin1I GRCGYC 1 cut(s) 17
Hin6I GCGC 2 cut(s) 4650, 4678
HinP1I GCGC 2 cut(s) 4650, 4678
HindIII AAGCTT 4 cut(s) 131, 1769, 3965, 4379
HpaII CCGG 8 cut(s) 864, 898, 1591, 1697, 2101, 3082, 3893, 4192
Hpy166II GTNNAC 9 cut(s) 468, 778, 942, 1635, 2274, 2628, 2684, 3141, 4365
Hpy8I GTNNAC 9 cut(s) 468, 778, 942, 1635, 2274, 2628, 2684, 3141, 4365
Hpy99I CGWCG 5 cut(s) 19, 25, 80, 83, 159
HpyCH4III ACNGT 9 cut(s) 1512, 2215, 2227, 2793, 2846, 3598, 3708, 4306, 4318
HpyCH4IV ACGT 5 cut(s) 754, 1582, 2145, 2781, 2941
HpySE526I ACGT 5 cut(s) 754, 1582, 2145, 2781, 2941
Hsp92I GRCGYC 1 cut(s) 17
HspAI GCGC 2 cut(s) 4650, 4678
Kpn2I TCCGGA 2 cut(s) 2100, 4191
KpnI GGTACC 1 cut(s) 2832
Ksp22I TGATCA 2 cut(s) 2206, 4297
KspI CCGCGG 1 cut(s) 568
LguI GCTCTTC 1 cut(s) 448
MaeI CTAG 8 cut(s) 164, 309, 378, 1218, 2807, 3414, 3615, 4641
MaeII ACGT 5 cut(s) 754, 1582, 2145, 2781, 2941
MbiI CCGCTC 1 cut(s) 458
MflI RGATCY 1 cut(s) 2103
MlyI GAGTC 5 cut(s) 1751, 1958, 2063, 2582, 4189
MmeI TCCRAC 5 cut(s) 1240, 1527, 2456, 3436, 4547
Mph1103I ATGCAT 5 cut(s) 1043, 2163, 3236, 3535, 4254
MroI TCCGGA 2 cut(s) 2100, 4191
MroXI GAANNNNTTC 2 cut(s) 2418, 4509
MspI CCGG 8 cut(s) 864, 898, 1591, 1697, 2101, 3082, 3893, 4192
MspR9I CCNGG 8 cut(s) 11, 142, 864, 865, 899, 1591, 2108, 3083
Mva1269I GAATGC 1 cut(s) 3174
MvaI CCWGG 3 cut(s) 11, 142, 2108
MvnI CGCG 1 cut(s) 567
NciI CCSGG 5 cut(s) 864, 865, 899, 1591, 3083
NcoI CCATGG 1 cut(s) 3790
NdeI CATATG 1 cut(s) 59
NmeAIII GCCGAG 1 cut(s) 867
NmuCI GTSAC 8 cut(s) 755, 797, 1235, 1910, 2015, 2527, 3833, 4106
NsiI ATGCAT 5 cut(s) 1043, 2163, 3236, 3535, 4254
OliI CACNNNNGTG 3 cut(s) 1917, 2022, 4113
PaeI GCATGC 2 cut(s) 2489, 4580
PaqCI CACCTGC 3 cut(s) 703, 1690, 2890
PasI CCCWGGG 1 cut(s) 141
PciI ACATGT 2 cut(s) 1130, 3323
PciSI GCTCTTC 1 cut(s) 448
PctI GAATGC 1 cut(s) 3174
PdmI GAANNNNTTC 2 cut(s) 2418, 4509
PflFI GACNNNGTC 1 cut(s) 19
PflMI CCANNNNNTGG 2 cut(s) 1259, 3455
PfoI TCCNGGA 1 cut(s) 2106
PleI GAGTC 5 cut(s) 1751, 1957, 2062, 2581, 4189
PpsI GAGTC 5 cut(s) 1751, 1957, 2062, 2581, 4189
Ppu21I YACGTR 2 cut(s) 755, 2942
PscI ACATGT 2 cut(s) 1130, 3323
PshAI GACNNNNGTC 4 cut(s) 1704, 2279, 3900, 4370
PsiI TTATAA 2 cut(s) 3662, 3714
Psp124BI GAGCTC 3 cut(s) 771, 934, 2958
Psp6I CCWGG 3 cut(s) 9, 140, 2106
PspEI GGTNACC 1 cut(s) 797
PspFI CCCAGC 3 cut(s) 1362, 3558, 3645
PspGI CCWGG 3 cut(s) 9, 140, 2106
PspPI GGNCC 7 cut(s) 33, 1706, 1937, 2042, 3902, 4021, 4133
PstI CTGCAG 5 cut(s) 390, 830, 2317, 3014, 4408
PstNI CAGNNNCTG 2 cut(s) 3078, 3390
PsuI RGATCY 1 cut(s) 2103
PsyI GACNNNGTC 1 cut(s) 19
PvuII CAGCTG 8 cut(s) 679, 825, 1817, 2060, 2122, 2866, 3390, 4213
RsaI GTAC 8 cut(s) 300, 369, 411, 815, 2166, 2790, 2830, 2999
RsaNI GTAC 8 cut(s) 299, 368, 410, 814, 2165, 2789, 2829, 2998
SacI GAGCTC 3 cut(s) 771, 934, 2958
SacII CCGCGG 1 cut(s) 568
SapI GCTCTTC 1 cut(s) 448
Sau96I GGNCC 7 cut(s) 33, 1706, 1937, 2042, 3902, 4021, 4133
SbfI CCTGCAGG 2 cut(s) 2317, 4408
SchI GAGTC 5 cut(s) 1751, 1958, 2063, 2582, 4189
ScrFI CCNGG 8 cut(s) 11, 142, 864, 865, 899, 1591, 2108, 3083
SdaI CCTGCAGG 2 cut(s) 2317, 4408
SfcI CTRYAG 9 cut(s) 386, 826, 1464, 1851, 1956, 2313, 3010, 4047, 4404
Sfr303I CCGCGG 1 cut(s) 568
SgrBI CCGCGG 1 cut(s) 568
SinI GGWCC 2 cut(s) 1706, 3902
SmaI CCCGGG 1 cut(s) 865
SphI GCATGC 2 cut(s) 2489, 4580
Sse8387I CCTGCAGG 2 cut(s) 2317, 4408
SspI AATATT 2 cut(s) 1801, 3997
SspMI CTAG 8 cut(s) 164, 309, 378, 1218, 2807, 3414, 3615, 4641
SstI GAGCTC 3 cut(s) 771, 934, 2958
StyD4I CCNGG 8 cut(s) 9, 140, 862, 863, 897, 1589, 2106, 3081
StyI CCWWGG 6 cut(s) 264, 333, 1253, 3449, 3699, 3790
TaaI ACNGT 9 cut(s) 1512, 2215, 2227, 2793, 2846, 3598, 3708, 4306, 4318
TaiI ACGT 5 cut(s) 757, 1585, 2148, 2784, 2944
TatI WGTACW 3 cut(s) 813, 2164, 2997
TauI GCSGC 3 cut(s) 52, 567, 570
TseFI GTSAC 8 cut(s) 755, 797, 1235, 1910, 2015, 2527, 3833, 4106
Tsp45I GTSAC 8 cut(s) 755, 797, 1235, 1910, 2015, 2527, 3833, 4106
TspGWI ACGGA 7 cut(s) 96, 172, 427, 675, 1521, 1750, 3559
TspMI CCCGGG 1 cut(s) 863
Tth111I GACNNNGTC 1 cut(s) 19
Van91I CCANNNNNTGG 2 cut(s) 1259, 3455
VneI GTGCAC 2 cut(s) 466, 2626
VpaK11BI GGWCC 2 cut(s) 1706, 3902
XagI CCTNNNNNAGG 1 cut(s) 145
XapI RAATTY 9 cut(s) 66, 1266, 1571, 1604, 2153, 2255, 3800, 4244, 4346
XbaI TCTAGA 3 cut(s) 1217, 2806, 3413
XcmI CCANNNNNNNNNTGG 4 cut(s) 1377, 1490, 3573, 3686
XmaI CCCGGG 1 cut(s) 863
XmiI GTMKAC 2 cut(s) 1634, 2683
XmnI GAANNNNTTC 2 cut(s) 2418, 4509
XspI CTAG 8 cut(s) 164, 309, 378, 1218, 2807, 3414, 3615, 4641
Zsp2I ATGCAT 5 cut(s) 1043, 2163, 3236, 3535, 4254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.