Rh1BG165100

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000280
Physical Location & Seq
Reverse (-)
26506145 .. 26514007
7863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG165100.1

Sequence Viewer

Length: 3180 bp
ATGAGCTTTTGTCTTTGGATGGTTGTCTACATACTGTCAATTTTCGTGTTGTGTAGGTATCCCCAGATGTCAGAGGAGGCTATTGCCGACGCTTGTCCTGTATGCCTTGGAAATTGCAATTGCAAAGCGTGCTTGCGTTTAGATGAAACTAATCATAATTTGAATAGCAAGCTTGCATTGCATGTTAGCAAAGATGAAGAAGTGGAGCACTCTAAGTATTTGCTTGAAGCATTGCTTCCATTCTTGAAAAGACTTAATGATGAACAAGTTATAGAGATGGTGATGGAGGCTAGAAGACAAGGGAAATCTCTTTCGGAGCTAAAGATCCGAAGGTCGGACAGCTCTCCTGATGATCGTGTATACTGCGACAACTGCAATACATCTATTATTGATTTCCACAGAAGCTGTCCTATATGTTCATATGACCTTTGTCTTATCTGCTGCCGGGAGATTCGAGATGGAAACCTGCAAGGAAGTGCTGAAGAAGTGATTGGGGAGTATATCAATCGAGGTCTAGACTATTTACATGGTGGAAAGGGCAACTTAATCAAAGGGCCTTCTGAAACTAAACTCAAGTGTCCTGTAAGGTCAACATCTAAGTGGAAAGTAAGCAAGGATCGAAGTATTTTTTGCCCCTCAAAAGACATGGACGGCTGTGGTTGTAGTGTTCTAGAACTGAGATGCATATTATCTGAAAACCACATCAGGGAGTTGGTAGAAAAAGCAGAAGAAATTGCAGAAACTGAAAAACTGATGTATGCAGCTGGACCTTCTGCACAGAAGTTTTCATGGTTCAACTCTGCTGGTGGTGCTGACTCAAGAAGCAATGAATTGACGAAAGCAGCTTCTCGAGAGGATTCGGAGGACAACTACTTGTACAGTCCAAGGGCTAAAAATATCCAACATGAAGACTTTAAGCATTTTCGCTGTCATTGGATCCGGGGTGAGCCTGTGATTGTGGGGAATGTGCTTGAAACTGCAACAGGTTTGAGCTGGGAACCATTTGTCATGTGGCGTGCTTGTCGTCAGATGAGAAATACCGGGCATGATAGACATGTGGAATTTAAGGCCATCGATTGCTTGGATTGGTGCGAGCTAGATATCGGTATCCACCAATTTTTTACTGGATATTTGGAGGGCCAGTTTGATGGGAAAATGTGGCCTCGTATTCTGAAACTCAACGATCGGTCCCTTGATGATTATTTGGAGAAGCGCCTTCCTCGTCATTGTGCTGAGTTTATTTGTTGCTTGCCATTTAAGGAATATACCCATCCTCGCAGTGGCCTACTGAACCTTGCTGTAAAATTGCCTATGGAGTGTGTAAAGCCAGACATGGGGCCAAAAATGGATATTGCTTATGGTGTGTCCCAGGAGCTTGGACGTGGAGATTCAGTAACTAAGCTTCACTGTGATTCATGTGATGTGGTGAATGTTTTGACACATACTGCTGAAGTGACGATTACTGCTGAGCAACTTTGTACTATAGAAGAGTTGAAAAAAGAGCACATTGAACAAGACCGAAGGGAAATGTTTGAAAATTATGAGGCTGTGGATGACAATGTTGATCATAAGGAGTCTGGCAGTGGGTCTTGTGTCAGGACTGCAAAGGGTAAACAATGTTGTACTCAAGTTAAGAATCAAAACAGGGTTGCAACTTTTCAAGAAAAGACTAAAGCGGCTGTTCAAACTGATAGTTGGAATGGTGGAAATTTTTCGAAGGAATCCATGTCTGCAAAGAATGCAGAGGGAGGTGCTTTATGGGACATTTTTCGACGACAGGATGTTCCTAAGCTGCAGGAATATCTATGGAAACACTACAAGGAATTCAGGCACACCTACTGTTGTCCATTGCAGCAGGTTACTCATCCCATACATGATCAGACTTTTTATCTGACATTAGAGCACAAGAGGAAGCTGAAGGAAGAATATGGAATTGAACCATGGACATTTATCCAGAAACTTGGAGATGCTGTCTTTATTCCTGCAGGATGTCCACACCAAGTTAGAAACTTGAAGTCGTGCATTAAAGTTTCTGTTGAATTTGTCTCCCCTGAAAATGTTGGTGAGTGCTTACGTCTGACAGATGAGTTCCGCACACTTCCACGAAAGCATATAGCTAAAGAGGACAAATTGGAGGTGAAGAAAATGATTGTTCATGCTGTACATGAGGCTGTGGAGATTTTGGATCAGAATGTGAGGTTATGTAGCGCTAAAAATTTCAGGGGAGAGAAGCCTTCACAGCCCCCTGTTAATATATGTAAGGGTAACATTGCAAACAAATCGAGGGAGAAAGCAGCTAGGGGACGACCACTAGAAGGAAGGGCAGAATTGAAAGAGGTTCAAATTCTTGAGGAGCAGGCTTCACAATTATCACAACCTATACAGCCCACTATTGATGTTGGTAGATTTGGAAGTACACGGAGAGAGGAAAAAGATGGAGGGCAACCACAAGAAGCAAGTAGAGTAGATGAAGAGGTCCAAATTCTTAAGGGGCAGCCTTCACAGTCAGCACAACATGTACAGCCCACTGCTTATGTTGATGGCAAGCATACAATGAGAAAGGAAACTGAGGGAGGGGTACTGCAAGAAACACATGGAGAAGTTGAAGACGTCCAAATTCTTGAGAAGCAGCCTTGGCAATCATCACAAACTGTACAAGCAGCTCTTCATGTTGGTAGCATTGAACATGCATCTAGAGAGGAAAAAGCTGGATGGCGACCACAAACTTGTGGAGAAGATGAAGAAGTCCAAATTCTTGAGGAGCAGCATTGGCAATCATCACAATCTGTACAAGCAACTCTTCACATTGGTAGCATTGCACATGCACTGAGAGAGGAAAAAGTGGGAGAACGACAGCATGAAGGAAAGGGAGAACAGGAAGAGGTCCGAAATCTTGGTGAGAAGCCTTCAAAGTTATCACAACCTGAACCTGTCCAGCCCACTGTTGATGTTGATAAAGCGGTGCAGCATCTGGATGAGCTCGTGGAGCTCCTTCGACATTTTGAACAGGGGAGGATGCCGATGGGAGGGAGAGATGGAAGAATCAGTTCTCAATTCCAGCTTTTATGCTCGGAGTTCTTGACTTTATCTTTGCATCTTGGTTATTCACCTAATGATACAAAACCTCACATTACACCATCTGCTCAGCAACTACATGATCCTCCAGTTGTAGATCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1059

Amino Acids

120.35

Weight (kDa)

5.89

Isoelectric Point (pI)

56.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjC PF02373 580 - 681 3.2e-14 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2613
Acc36I ACCTGC 2 cut(s) 474, 1846
AccI GTMKAC 2 cut(s) 27, 360
AciI CCGC 3 cut(s) 1676, 2092, 2960
AclWI GGATC 6 cut(s) 319, 624, 931, 944, 2193, 3152
AcsI RAATTY 9 cut(s) 1061, 1708, 1823, 2039, 2215, 2343, 2481, 2616, 2751
AcuI CTGAAG 3 cut(s) 501, 1470, 1937
AcyI GRCGYC 1 cut(s) 2610
AfaI GTAC 9 cut(s) 878, 1480, 1624, 2163, 2416, 2520, 2580, 2655, 2790
AfeI AGCGCT 1 cut(s) 2209
AfiI CCNNNNNNNGG 6 cut(s) 334, 706, 1280, 1334, 2315, 3026
AflII CTTAAG 1 cut(s) 2486
AflIII ACRYGT 2 cut(s) 1054, 2515
AjiI CACGTC 1 cut(s) 1382
AjnI CCWGG 1 cut(s) 1368
AjuI GAANNNNNNNTTGG 2 cut(s) 474, 506
Alw21I GWGCWC 5 cut(s) 210, 1506, 1905, 2982, 2991
Alw26I GTCTC 1 cut(s) 2050
AlwI GGATC 6 cut(s) 319, 624, 931, 944, 2193, 3152
AlwNI CAGNNNCTG 3 cut(s) 405, 743, 2971
Ama87I CYCGRG 1 cut(s) 849
Aor51HI AGCGCT 1 cut(s) 2209
AoxI GGCC 6 cut(s) 554, 1068, 1138, 1160, 1282, 1337
ApoI RAATTY 9 cut(s) 1061, 1708, 1823, 2039, 2215, 2343, 2481, 2616, 2751
ArsI GACNNNNNNTTYG 2 cut(s) 3073, 3105
Asp700I GAANNNNTTC 2 cut(s) 1711, 3046
AspLEI GCGC 2 cut(s) 1215, 2210
AspS9I GGNCC 7 cut(s) 554, 767, 1138, 1188, 1337, 2476, 2884
AsuC2I CCSGG 3 cut(s) 446, 941, 1042
AsuHPI GGTGA 7 cut(s) 292, 956, 1438, 2075, 2149, 2909, 3099
AsuII TTCGAA 1 cut(s) 1715
AvaI CYCGRG 1 cut(s) 849
AvaII GGWCC 4 cut(s) 767, 1188, 2476, 2884
BamHI GGATCC 1 cut(s) 936
BanII GRGCYC 2 cut(s) 2982, 2991
BarI GAAGNNNNNNTAC 2 cut(s) 1386, 1418
BauI CACGAG 1 cut(s) 2981
BbsI GAAGAC 3 cut(s) 301, 915, 2613
Bbv12I GWGCWC 5 cut(s) 210, 1506, 1905, 2982, 2991
BceAI ACGGC 1 cut(s) 667
BcgI CGANNNNNNTGC 2 cut(s) 2262, 2296
BciT130I CCWGG 1 cut(s) 1370
BciVI GTATCC 2 cut(s) 69, 1118
BclI TGATCA 2 cut(s) 1564, 1876
BcnI CCSGG 3 cut(s) 446, 941, 1042
BcoDI GTCTC 1 cut(s) 2050
BfaI CTAG 7 cut(s) 291, 515, 671, 1097, 2298, 2312, 2694
BfmI CTRYAG 3 cut(s) 1482, 1793, 1983
BfoI RGCGCY 2 cut(s) 1216, 2211
BfrI CTTAAG 1 cut(s) 2486
BfuAI ACCTGC 2 cut(s) 474, 1846
BfuI GTATCC 2 cut(s) 69, 1118
BglII AGATCT 1 cut(s) 3172
BlpI GCTNAGC 2 cut(s) 1467, 3144
Bme1390I CCNGG 4 cut(s) 446, 941, 1042, 1370
Bme18I GGWCC 4 cut(s) 767, 1188, 2476, 2884
BmeT110I CYCGRG 1 cut(s) 849
BmgBI CACGTC 1 cut(s) 1382
BmgT120I GGNCC 7 cut(s) 554, 767, 1138, 1188, 1337, 2476, 2884
BmiI GGNNCC 4 cut(s) 938, 999, 1190, 1338
BmrFI CCNGG 4 cut(s) 446, 941, 1042, 1370
BmsI GCATC 6 cut(s) 671, 1957, 2699, 2977, 3006, 3103
BoxI GACNNNNGTC 2 cut(s) 93, 429
BpiI GAAGAC 3 cut(s) 301, 915, 2613
BpmI CTGGAG 1 cut(s) 3147
Bpu10I CCTNAGC 1 cut(s) 1788
Bpu1102I GCTNAGC 2 cut(s) 1467, 3144
Bpu14I TTCGAA 1 cut(s) 1715
BpuEI CTTGAG 6 cut(s) 557, 802, 1611, 2369, 2642, 2777
BpuMI CCSGG 3 cut(s) 446, 941, 1042
Bsa29I ATCGAT 1 cut(s) 1074
BsaHI GRCGYC 1 cut(s) 2610
BsaJI CCNNGG 6 cut(s) 106, 884, 940, 1368, 1940, 2633
BsaXI ACNNNNNCTCC 6 cut(s) 1127, 1157, 1377, 1407, 1956, 1986
Bsc4I CCNNNNNNNGG 6 cut(s) 334, 706, 1280, 1334, 2315, 3026
Bse1I ACTGG 3 cut(s) 1129, 1141, 3164
Bse3DI GCAATG 6 cut(s) 176, 230, 832, 1847, 2268, 2814
BseBI CCWGG 1 cut(s) 1370
BseCI ATCGAT 1 cut(s) 1074
BseDI CCNNGG 6 cut(s) 106, 884, 940, 1368, 1940, 2633
BseGI GGATG 9 cut(s) 24, 1270, 1558, 1786, 1864, 1994, 2717, 2980, 3021
BseLI CCNNNNNNNGG 6 cut(s) 334, 706, 1280, 1334, 2315, 3026
BseMI GCAATG 6 cut(s) 176, 230, 832, 1847, 2268, 2814
BseMII CTCAG 6 cut(s) 668, 1224, 1458, 2559, 2819, 3158
BseNI ACTGG 3 cut(s) 1129, 1141, 3164
BseRI GAGGAG 3 cut(s) 89, 2366, 2774
BseYI CCCAGC 1 cut(s) 993
BsgI GTGCAG 2 cut(s) 759, 2984
Bsh1285I CGRYCG 1 cut(s) 1186
BshFI GGCC 6 cut(s) 556, 1070, 1140, 1162, 1284, 1339
BshVI ATCGAT 1 cut(s) 1074
BsiEI CGRYCG 1 cut(s) 1186
BsiHKAI GWGCWC 5 cut(s) 210, 1506, 1905, 2982, 2991
BsiHKCI CYCGRG 1 cut(s) 849
BsiSI CCGG 3 cut(s) 445, 940, 1041
BslFI GGGAC 4 cut(s) 1174, 1351, 1775, 2316
BslI CCNNNNNNNGG 6 cut(s) 334, 706, 1280, 1334, 2315, 3026
BsmAI GTCTC 1 cut(s) 2050
BsmFI GGGAC 4 cut(s) 1174, 1351, 1775, 2316
BsmI GAATGC 1 cut(s) 1744
BsnI GGCC 6 cut(s) 556, 1070, 1140, 1162, 1284, 1339
BsoBI CYCGRG 1 cut(s) 849
Bsp119I TTCGAA 1 cut(s) 1715
Bsp1286I GDGCHC 5 cut(s) 210, 1506, 1905, 2982, 2991
Bsp1407I TGTACA 5 cut(s) 876, 2161, 2518, 2653, 2788
Bsp1720I GCTNAGC 2 cut(s) 1467, 3144
Bsp19I CCATGG 1 cut(s) 1940
BspACI CCGC 3 cut(s) 1676, 2092, 2960
BspANI GGCC 6 cut(s) 556, 1070, 1140, 1162, 1284, 1339
BspCNI CTCAG 6 cut(s) 669, 1225, 1459, 2560, 2820, 3157
BspDI ATCGAT 1 cut(s) 1074
BspLI GGNNCC 4 cut(s) 938, 999, 1190, 1338
BspMAI CTGCAG 2 cut(s) 1797, 1987
BspMI ACCTGC 2 cut(s) 474, 1846
BspPI GGATC 6 cut(s) 319, 624, 931, 944, 2193, 3152
BspQI GCTCTTC 1 cut(s) 2670
BspT104I TTCGAA 1 cut(s) 1715
BspTI CTTAAG 1 cut(s) 2486
BsrDI GCAATG 6 cut(s) 176, 230, 832, 1847, 2268, 2814
BsrGI TGTACA 5 cut(s) 876, 2161, 2518, 2653, 2788
BsrI ACTGG 3 cut(s) 1129, 1141, 3164
BssECI CCNNGG 6 cut(s) 106, 884, 940, 1368, 1940, 2633
BssNAI GTATAC 1 cut(s) 361
BssNI GRCGYC 1 cut(s) 2610
BssSI CACGAG 1 cut(s) 2981
BssT1I CCWWGG 4 cut(s) 106, 884, 1940, 2633
Bst1107I GTATAC 1 cut(s) 361
Bst2BI CACGAG 1 cut(s) 2981
Bst2UI CCWGG 1 cut(s) 1370
Bst4CI ACNGT 7 cut(s) 36, 881, 1409, 1841, 2505, 2653, 2944
Bst6I CTCTTC 5 cut(s) 1482, 2466, 2670, 2805, 2874
BstACI GRCGYC 1 cut(s) 2610
BstAFI CTTAAG 1 cut(s) 2486
BstAPI GCANNNNNTGC 2 cut(s) 129, 1739
BstAUI TGTACA 5 cut(s) 876, 2161, 2518, 2653, 2788
BstBI TTCGAA 1 cut(s) 1715
BstC8I GCNNGC 9 cut(s) 130, 134, 170, 174, 1017, 1094, 1250, 2358, 2546
BstDSI CCRYGG 1 cut(s) 1940
BstF5I GGATG 9 cut(s) 24, 1270, 1558, 1786, 1864, 1994, 2717, 2980, 3021
BstH2I RGCGCY 2 cut(s) 1216, 2211
BstHHI GCGC 2 cut(s) 1215, 2210
BstMAI GTCTC 1 cut(s) 2050
BstMCI CGRYCG 1 cut(s) 1186
BstMWI GCNNNNNNNGC 9 cut(s) 129, 178, 372, 809, 1739, 2239, 2635, 2770, 2986
BstNI CCWGG 1 cut(s) 1370
BstNSI RCATGY 5 cut(s) 185, 1058, 2519, 2690, 2825
BstPAI GACNNNNGTC 2 cut(s) 93, 429
BstSCI CCNGG 4 cut(s) 444, 939, 1040, 1368
BstSFI CTRYAG 3 cut(s) 1482, 1793, 1983
BstV2I GAAGAC 3 cut(s) 301, 915, 2613
BstX2I RGATCY 3 cut(s) 324, 936, 3172
BstXI CCANNNNNNTGG 3 cut(s) 1148, 1376, 1961
BstYI RGATCY 3 cut(s) 324, 936, 3172
BstZ17I GTATAC 1 cut(s) 361
Bsu15I ATCGAT 1 cut(s) 1074
BsuI GTATCC 2 cut(s) 69, 1118
BsuRI GGCC 6 cut(s) 556, 1070, 1140, 1162, 1284, 1339
BsuTUI ATCGAT 1 cut(s) 1074
BtgI CCRYGG 1 cut(s) 1940
BtrI CACGTC 1 cut(s) 1382
BtsCI GGATG 9 cut(s) 24, 1270, 1558, 1786, 1864, 1994, 2717, 2980, 3021
BtsI GCAGTG 3 cut(s) 1285, 1588, 2526
BtsIMutI CAGTG 6 cut(s) 1285, 1405, 1588, 2526, 2825, 2940
BveI ACCTGC 2 cut(s) 474, 1846
Cac8I GCNNGC 9 cut(s) 130, 134, 170, 174, 1017, 1094, 1250, 2358, 2546
CaiI CAGNNNCTG 3 cut(s) 405, 743, 2971
CfoI GCGC 2 cut(s) 1215, 2210
Cfr13I GGNCC 7 cut(s) 554, 767, 1138, 1188, 1337, 2476, 2884
ClaI ATCGAT 1 cut(s) 1074
CseI GACGC 1 cut(s) 98
Csp6I GTAC 9 cut(s) 877, 1479, 1623, 2162, 2415, 2519, 2579, 2654, 2789
CviQI GTAC 9 cut(s) 877, 1479, 1623, 2162, 2415, 2519, 2579, 2654, 2789
Eam1104I CTCTTC 5 cut(s) 1482, 2466, 2670, 2805, 2874
EarI CTCTTC 5 cut(s) 1482, 2466, 2670, 2805, 2874
Ecl136II GAGCTC 2 cut(s) 2980, 2989
Eco130I CCWWGG 4 cut(s) 106, 884, 1940, 2633
Eco24I GRGCYC 2 cut(s) 2982, 2991
Eco32I GATATC 1 cut(s) 1102
Eco47I GGWCC 4 cut(s) 767, 1188, 2476, 2884
Eco47III AGCGCT 1 cut(s) 2209
Eco53kI GAGCTC 2 cut(s) 2980, 2989
Eco57I CTGAAG 3 cut(s) 501, 1470, 1937
Eco88I CYCGRG 1 cut(s) 849
EcoICRI GAGCTC 2 cut(s) 2980, 2989
EcoO109I RGGNCCY 1 cut(s) 554
EcoRI GAATTC 1 cut(s) 1823
EcoRII CCWGG 1 cut(s) 1368
EcoRV GATATC 1 cut(s) 1102
EcoT14I CCWWGG 4 cut(s) 106, 884, 1940, 2633
EcoT22I ATGCAT 2 cut(s) 686, 2692
EcoT38I GRGCYC 2 cut(s) 2982, 2991
ErhI CCWWGG 4 cut(s) 106, 884, 1940, 2633
FalI AAGNNNNNCTT 8 cut(s) 219, 251, 527, 559, 2649, 2681, 2784, 2816
FaqI GGGAC 4 cut(s) 1174, 1351, 1775, 2316
FauNDI CATATG 1 cut(s) 421
FbaI TGATCA 2 cut(s) 1564, 1876
FblI GTMKAC 2 cut(s) 27, 360
FokI GGATG 9 cut(s) 31, 1257, 1565, 1793, 1851, 2001, 2724, 2987, 3028
FriOI GRGCYC 2 cut(s) 2982, 2991
FspBI CTAG 7 cut(s) 291, 515, 671, 1097, 2298, 2312, 2694
GlaI GCGC 2 cut(s) 1214, 2209
GsaI CCCAGC 1 cut(s) 997
GsuI CTGGAG 1 cut(s) 3147
HaeII RGCGCY 2 cut(s) 1216, 2211
HaeIII GGCC 6 cut(s) 556, 1070, 1140, 1162, 1284, 1339
HapII CCGG 3 cut(s) 445, 940, 1041
HgaI GACGC 1 cut(s) 98
HhaI GCGC 2 cut(s) 1215, 2210
Hin1I GRCGYC 1 cut(s) 2610
Hin6I GCGC 2 cut(s) 1213, 2208
HinP1I GCGC 2 cut(s) 1213, 2208
HincII GTYRAC 1 cut(s) 591
HindII GTYRAC 1 cut(s) 591
HindIII AAGCTT 2 cut(s) 170, 1400
HinfI GANTC 9 cut(s) 451, 815, 857, 1388, 1412, 1574, 1636, 1721, 3042
HpaII CCGG 3 cut(s) 445, 940, 1041
HphI GGTGA 7 cut(s) 292, 956, 1438, 2075, 2149, 2909, 3099
Hpy166II GTNNAC 6 cut(s) 28, 361, 591, 1613, 1994, 2417
Hpy8I GTNNAC 6 cut(s) 28, 361, 591, 1613, 1994, 2417
Hpy99I CGWCG 2 cut(s) 92, 1776
HpyCH4III ACNGT 7 cut(s) 36, 881, 1409, 1841, 2505, 2653, 2944
HpyCH4IV ACGT 3 cut(s) 1381, 2074, 2610
HpyF10VI GCNNNNNNNGC 9 cut(s) 129, 178, 372, 809, 1739, 2239, 2635, 2770, 2986
HpySE526I ACGT 3 cut(s) 1381, 2074, 2610
Hsp92I GRCGYC 1 cut(s) 2610
HspAI GCGC 2 cut(s) 1213, 2208
Ksp22I TGATCA 2 cut(s) 1564, 1876
LguI GCTCTTC 1 cut(s) 2670
LmnI GCTCC 7 cut(s) 205, 316, 1372, 2353, 2761, 2986, 2994
LweI GCATC 6 cut(s) 671, 1957, 2699, 2977, 3006, 3103
MaeI CTAG 7 cut(s) 291, 515, 671, 1097, 2298, 2312, 2694
MaeII ACGT 3 cut(s) 1381, 2074, 2610
MaeIII GTNAC 4 cut(s) 1393, 1453, 1858, 2264
MfeI CAATTG 1 cut(s) 118
MflI RGATCY 3 cut(s) 324, 936, 3172
MhlI GDGCHC 5 cut(s) 210, 1506, 1905, 2982, 2991
MlyI GAGTC 2 cut(s) 809, 1583
MmeI TCCRAC 3 cut(s) 315, 925, 1676
Mph1103I ATGCAT 2 cut(s) 686, 2692
MroXI GAANNNNTTC 2 cut(s) 1711, 3046
MseI TTAA 9 cut(s) 255, 545, 915, 1065, 1257, 1632, 2025, 2250, 2487
MslI CAYNNNNRTG 2 cut(s) 598, 2973
MspA1I CMGCKG 1 cut(s) 764
MspCI CTTAAG 1 cut(s) 2486
MspI CCGG 3 cut(s) 445, 940, 1041
MspR9I CCNGG 4 cut(s) 446, 941, 1042, 1370
MunI CAATTG 1 cut(s) 118
Mva1269I GAATGC 1 cut(s) 1744
MvaI CCWGG 1 cut(s) 1370
MwoI GCNNNNNNNGC 9 cut(s) 129, 178, 372, 809, 1739, 2239, 2635, 2770, 2986
NciI CCSGG 3 cut(s) 446, 941, 1042
NcoI CCATGG 1 cut(s) 1940
NdeI CATATG 1 cut(s) 421
NlaIV GGNNCC 4 cut(s) 938, 999, 1190, 1338
NmuCI GTSAC 1 cut(s) 1453
NsiI ATGCAT 2 cut(s) 686, 2692
NspI RCATGY 5 cut(s) 185, 1058, 2519, 2690, 2825
NspV TTCGAA 1 cut(s) 1715
PaeR7I CTCGAG 1 cut(s) 849
PciI ACATGT 2 cut(s) 1054, 2515
PciSI GCTCTTC 1 cut(s) 2670
PctI GAATGC 1 cut(s) 1744
PdmI GAANNNNTTC 2 cut(s) 1711, 3046
PfeI GAWTC 7 cut(s) 451, 857, 1388, 1412, 1636, 1721, 3042
Ple19I CGATCG 1 cut(s) 1186
PleI GAGTC 2 cut(s) 809, 1582
PpsI GAGTC 2 cut(s) 809, 1582
PscI ACATGT 2 cut(s) 1054, 2515
PshAI GACNNNNGTC 2 cut(s) 93, 429
Psp124BI GAGCTC 2 cut(s) 2982, 2991
Psp6I CCWGG 1 cut(s) 1368
PspFI CCCAGC 1 cut(s) 993
PspGI CCWGG 1 cut(s) 1368
PspN4I GGNNCC 4 cut(s) 938, 999, 1190, 1338
PspPI GGNCC 7 cut(s) 554, 767, 1138, 1188, 1337, 2476, 2884
PstI CTGCAG 2 cut(s) 1797, 1987
PstNI CAGNNNCTG 3 cut(s) 405, 743, 2971
PsuI RGATCY 3 cut(s) 324, 936, 3172
PvuI CGATCG 1 cut(s) 1186
PvuII CAGCTG 1 cut(s) 764
RsaI GTAC 9 cut(s) 878, 1480, 1624, 2163, 2416, 2520, 2580, 2655, 2790
RsaNI GTAC 9 cut(s) 877, 1479, 1623, 2162, 2415, 2519, 2579, 2654, 2789
RseI CAYNNNNRTG 2 cut(s) 598, 2973
SacI GAGCTC 2 cut(s) 2982, 2991
SapI GCTCTTC 1 cut(s) 2670
SaqAI TTAA 9 cut(s) 255, 545, 915, 1065, 1257, 1632, 2025, 2250, 2487
Sau96I GGNCC 7 cut(s) 554, 767, 1138, 1188, 1337, 2476, 2884
SbfI CCTGCAGG 1 cut(s) 1987
SchI GAGTC 2 cut(s) 809, 1583
ScrFI CCNGG 4 cut(s) 446, 941, 1042, 1370
SdaI CCTGCAGG 1 cut(s) 1987
SduI GDGCHC 5 cut(s) 210, 1506, 1905, 2982, 2991
SfaNI GCATC 6 cut(s) 671, 1957, 2699, 2977, 3006, 3103
SfcI CTRYAG 3 cut(s) 1482, 1793, 1983
Sfr274I CTCGAG 1 cut(s) 849
SfuI TTCGAA 1 cut(s) 1715
SinI GGWCC 4 cut(s) 767, 1188, 2476, 2884
SlaI CTCGAG 1 cut(s) 849
SmiMI CAYNNNNRTG 2 cut(s) 598, 2973
SmlI CTYRAG 8 cut(s) 572, 817, 849, 1626, 2348, 2486, 2621, 2756
SmoI CTYRAG 8 cut(s) 572, 817, 849, 1626, 2348, 2486, 2621, 2756
Sse8387I CCTGCAGG 1 cut(s) 1987
SsiI CCGC 3 cut(s) 1676, 2092, 2960
SspMI CTAG 7 cut(s) 291, 515, 671, 1097, 2298, 2312, 2694
SstI GAGCTC 2 cut(s) 2982, 2991
StyD4I CCNGG 4 cut(s) 444, 939, 1040, 1368
StyI CCWWGG 4 cut(s) 106, 884, 1940, 2633
TaaI ACNGT 7 cut(s) 36, 881, 1409, 1841, 2505, 2653, 2944
TaiI ACGT 3 cut(s) 1384, 2077, 2613
TaqI TCGA 9 cut(s) 454, 508, 619, 850, 1074, 1715, 1771, 2282, 2995
TaqII GACCGA 2 cut(s) 1176, 1533
TatI WGTACW 8 cut(s) 876, 1478, 1622, 2161, 2414, 2518, 2653, 2788
TauI GCSGC 1 cut(s) 1679
TfiI GAWTC 7 cut(s) 451, 857, 1388, 1412, 1636, 1721, 3042
Tru1I TTAA 9 cut(s) 255, 545, 915, 1065, 1257, 1632, 2025, 2250, 2487
Tru9I TTAA 9 cut(s) 255, 545, 915, 1065, 1257, 1632, 2025, 2250, 2487
TscAI CASTG 6 cut(s) 1285, 1412, 1588, 2533, 2832, 2947
TseFI GTSAC 1 cut(s) 1453
Tsp45I GTSAC 1 cut(s) 1453
TspGWI ACGGA 1 cut(s) 2434
TspRI CASTG 6 cut(s) 1285, 1412, 1588, 2533, 2832, 2947
Vha464I CTTAAG 1 cut(s) 2486
VpaK11BI GGWCC 4 cut(s) 767, 1188, 2476, 2884
XapI RAATTY 9 cut(s) 1061, 1708, 1823, 2039, 2215, 2343, 2481, 2616, 2751
XbaI TCTAGA 3 cut(s) 514, 670, 2693
XceI RCATGY 5 cut(s) 185, 1058, 2519, 2690, 2825
XcmI CCANNNNNNNNNTGG 4 cut(s) 1008, 1078, 1121, 1277
XhoI CTCGAG 1 cut(s) 849
XmiI GTMKAC 2 cut(s) 27, 360
XmnI GAANNNNTTC 2 cut(s) 1711, 3046
XspI CTAG 7 cut(s) 291, 515, 671, 1097, 2298, 2312, 2694
ZraI GACGTC 1 cut(s) 2611
Zsp2I ATGCAT 2 cut(s) 686, 2692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.