Rroxscaffold_3G00235210

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000280
Physical Location & Seq
Reverse (-)
22088217 .. 22091126
2910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00235210.1

Sequence Viewer

Length: 2910 bp
ATGGCGGCGAGAAAGAAAACCAAAGGCCGAGGCCGGCCGAGGCAAGAAGAGAACGGCGCTGGTCTGGAGGAGATGAAGATGAAGGCCGAGGGTATTTTGGGAATTCAGAACGAGGGAGCTAATGGGGAAGTAATAGAAGAAGCTGTGGAGCCGAAGCCAGAGTCGGCTCCTTTGGTGGAGGGCAGACGCAGGAAGCGGCGGAGGAAGAAACAAGAAGAAGGTGGTGGTGGTGGTGAGGGTATTTTGGGAAATCAAAACGAGGAAGAGGTTGCTGAATATGGTGTGGAGGTAAAGAGGCGTTGTCCACAGAGGGCGGCAAGGGCTGAAAAGGCAGTGGTGGAGGAGGAGGAGGAGCCGCTGATTCGGAAGATGAAGAAGATGAAGAAGAGAAAAGCAGCGGCGGCGGAGGGGGAGGAAGATGAGGCAGTGAAGGACGTTTCAACAATGTGCCATCAGTGTCAGAGGAATGATAAAGGGAGAGTGGTTCGGTGCACGAGTTGTCCGAAAAGGGGTAAGGGAATGAGGAAGAGGTATTGTATCCCTTGCATACAGAGGTGGTATCCCAACACATCGGAGGAAGCCATTGCTGAGGCTTGTCCTGTGTGCCGCGGCAACTGCAACTGCAAAGCCTGCCTTCGACTGGATGTGCCTAATAGGTGTTTGAAGAATCTAGACCCGAAAATCGACGAGGAGACCAAGCTGGAGCACTGTAAGTATTTGGTTCATCAGCTGCTTCCGTATTTGAAGAGGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTGGAAGCCAAGAGGCAGGGATTAAGTGATGTTTCAGAGCTCAAGGTGGAGAAGGCTAATTGCGCTGCCGATGAGCGTATGTACTGCAATAACTGCAGCACTTCGATTTTTGATTTTCATAGGAGTTGTCCTGAGTGTGAGTATGATCTGTGCCTCATTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAAGAAGAGAGGTATGGAGGAGGAAGACAGTCAGAAGTATGTCGATTTGGGGCGTGAGTATTTGCATGGAAAGGGAGACTTGAAGAGCTTGCTCAAAAAAAAGAGAGAAAAGGAGTTGAAGACCTTGCTAAAGAATGAAAGAGAAGAAGAGTTGAAGGAGAATGTTGATGTGGGAGTGAAGCTGGACTCGTCAGAATCTGCTCAAGAGTCCACGATAGCAAGTGAAGATGGAAATATTCAAGATGGGAAGCAGAAGAAGAGAGGCAGAGAGGATGAAGTAAGTGAGGAATTTGTTAATGCGGGGGAGTGTTTGGATGGAGAGGGAGAGCTGAAGAGGAATGATGATGTGGGAGTGAAGCAGGACGAGTCCGAATCTGCTGAAGAGTCCACATTTGAGTGGAGAGCAAGTGAAGATGGAAGCATTCTCTGCCCCCCAACACACATGAAAGGTTGTGGTAAGCATAATTTAGAATTGAGGTGCAATTTTTCTCAGAATCATGTCATAGACTTGGTAGAGAAAGCAGGAAAAATAGATGAAAGTTACAATCCTATGCATGCTAGTAGTGAAACATGTGCACAAAAGTGTTCATGTGTTAAACCTGTGAATGATGCTGTCAACTCAAGTAGCAGCAGCTGTAAGTTAAGAAAAGCAGCTTCTAGAGACGGTTCTGATGATAACTACTTGTTCTGTCCAAGGGTCGGAGAAATCCAAGATGAGGATTTTGGGCATTTTCAGTGGCATTGGATGAGGGGTGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCAGGTTTGAGCTGGGAACCATTAGTCATGTGGCGTGCATTTAGGCAGATAAAGAATCTCAAGCATGATAGAGAATTGGCAGTCCATACCATTGAATGCTTGGATTGGAGTTTCAAAGAAACTAATAATCACCATTTCTTTACTGGATATTCCGAAGGCCGGCCTGATGAGAAAGGATGGCCTGAGATTCTCAAGCTCAAAGACTTTCCGCCATCTACTGAGTTTGACAATCGTCTCCCCCGGCATGGTAAGGAATTTGTCTGTTGTCTGCCTTTCAAAGAGTATACTCACCCCAACAAGGGAACCCTGAACCTTGCTATCCACTTGCCACCTACCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACGGGGAGATTCTGTGACCAAGCTTCACTGCGATATGTCTGATGCGGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACAACTGAACAACTTGCAACTATAGAGAAGTTGAAAAAAAGGCACAGGAAGCAAGACGAAAGAGAGATATTTGGGAATTGTTACACAAGTGCTGGAAATGGAAGTGGGCCTCAGGAAGAATCAGCTGAAGTATGTGAGGATGGTGGTGCCTTATGGGACATTTTCCGGATCCGGGATCTTCCGCAGCTGGAGAAATACATCCACAAGCACATCAATGAATTTAGGCATGTGCACTGTAATCCTTTGCAGCGTGTTACTCATGCTATACATGATCAGACTGTTTATCTTACTGTGGAGCACAAGAGGAAGCTCAAGGAGGAATTTGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTGGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGCCAAACCACAGGGCCAGCGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTGTGAAGGAGATTTCTGGTGAGAATGCGAAGCCTGAGGATGTGAAGCCTAAGAGGAGGTGCAAGGGGCCTAAGAGGAGGCGCAAGGGGAACAAAGGAGAGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

969

Amino Acids

110.21

Weight (kDa)

6.71

Isoelectric Point (pI)

55.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 146 - 213 1e-06 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 816 - 892 1.3e-12 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 754
Acc36I ACCTGC 2 cut(s) 754, 2609
AccB1I GGYRCC 1 cut(s) 2384
AccI GTMKAC 1 cut(s) 2042
AccII CGCG 1 cut(s) 609
AccIII TCCGGA 1 cut(s) 2403
AclWI GGATC 4 cut(s) 1765, 2401, 2414, 2421
AcoI YGGCCR 1 cut(s) 35
AcsI RAATTY 5 cut(s) 102, 1256, 2012, 2456, 2558
AcuI CTGAAG 4 cut(s) 1319, 1368, 2247, 2385
AfaI GTAC 1 cut(s) 863
AflIII ACRYGT 1 cut(s) 1538
AleI CACNNNNGTG 1 cut(s) 1549
Alw21I GWGCWC 6 cut(s) 494, 708, 822, 1546, 2472, 2538
Alw26I GTCTC 4 cut(s) 686, 1038, 1623, 1997
Alw44I GTGCAC 3 cut(s) 490, 1542, 2468
AlwI GGATC 4 cut(s) 1765, 2401, 2414, 2421
AlwNI CAGNNNCTG 2 cut(s) 1166, 1602
Aor13HI TCCGGA 1 cut(s) 2403
ApaLI GTGCAC 3 cut(s) 490, 1542, 2468
ApoI RAATTY 5 cut(s) 102, 1256, 2012, 2456, 2558
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
Asp700I GAANNNNTTC 1 cut(s) 2721
AspLEI GCGC 3 cut(s) 59, 845, 2886
AspS9I GGNCC 5 cut(s) 2114, 2233, 2345, 2748, 2870
AsuC2I CCSGG 3 cut(s) 947, 1999, 2411
AsuHPI GGTGA 9 cut(s) 245, 778, 1733, 1880, 2039, 2647, 2708, 2782, 2834
AvaII GGWCC 1 cut(s) 2114
AxyI CCTNAGG 2 cut(s) 2349, 2838
BaeGI GKGCMC 3 cut(s) 494, 1546, 2472
BamHI GGATCC 1 cut(s) 2406
BanI GGYRCC 1 cut(s) 2384
BanII GRGCYC 1 cut(s) 822
BauI CACGAG 1 cut(s) 493
BbsI GAAGAC 2 cut(s) 999, 1094
Bbv12I GWGCWC 6 cut(s) 494, 708, 822, 1546, 2472, 2538
BbvCI CCTCAGC 1 cut(s) 588
BceAI ACGGC 2 cut(s) 70, 2557
BciVI GTATCC 2 cut(s) 548, 570
BclI TGATCA 1 cut(s) 2509
BcnI CCSGG 3 cut(s) 947, 1999, 2411
BcoDI GTCTC 4 cut(s) 686, 1038, 1623, 1997
BfaI CTAG 3 cut(s) 671, 1527, 1626
BfmI CTRYAG 3 cut(s) 874, 2259, 2616
BfoI RGCGCY 1 cut(s) 60
BfuAI ACCTGC 2 cut(s) 754, 2609
BfuI GTATCC 2 cut(s) 548, 570
Bme1390I CCNGG 3 cut(s) 947, 1999, 2411
Bme18I GGWCC 1 cut(s) 2114
BmgT120I GGNCC 5 cut(s) 2114, 2233, 2345, 2748, 2870
BmiI GGNNCC 9 cut(s) 150, 168, 354, 1776, 2062, 2115, 2386, 2408, 2871
BmrFI CCNGG 3 cut(s) 947, 1999, 2411
BmsI GCATC 3 cut(s) 1567, 2188, 2590
BoxI GACNNNNGTC 3 cut(s) 1989, 2112, 2582
BpiI GAAGAC 2 cut(s) 999, 1094
BpmI CTGGAG 4 cut(s) 86, 722, 2447, 2786
Bpu10I CCTNAGC 1 cut(s) 588
BpuEI CTTGAG 6 cut(s) 806, 1155, 1573, 1802, 1934, 2534
BpuMI CCSGG 3 cut(s) 947, 1999, 2411
BsaI GGTCTC 1 cut(s) 686
BsaJI CCNNGG 7 cut(s) 28, 38, 87, 607, 1661, 1997, 2573
BsaWI WCCGGW 1 cut(s) 2403
BsaXI ACNNNNNCTCC 6 cut(s) 1360, 1390, 2154, 2184, 2589, 2619
Bse118I RCCGGY 2 cut(s) 33, 1917
Bse1I ACTGG 3 cut(s) 645, 1906, 2673
Bse21I CCTNAGG 2 cut(s) 2349, 2838
Bse3DI GCAATG 2 cut(s) 582, 1747
BseAI TCCGGA 1 cut(s) 2403
BseDI CCNNGG 7 cut(s) 28, 38, 87, 607, 1661, 1997, 2573
BseGI GGATG 9 cut(s) 649, 763, 1246, 1288, 1719, 1940, 2383, 2436, 2848
BseMI GCAATG 2 cut(s) 582, 1747
BseMII CTCAG 9 cut(s) 579, 903, 1472, 1932, 1968, 2109, 2363, 2676, 2829
BseNI ACTGG 3 cut(s) 645, 1906, 2673
BseRI GAGGAG 9 cut(s) 83, 356, 359, 362, 365, 704, 1001, 2872, 2893
BseSI GKGCMC 3 cut(s) 494, 1546, 2472
BseX3I CGGCCG 1 cut(s) 35
BseYI CCCAGC 1 cut(s) 1770
Bsh1236I CGCG 1 cut(s) 609
Bsh1285I CGRYCG 1 cut(s) 38
BshNI GGYRCC 1 cut(s) 2384
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 6 cut(s) 494, 708, 822, 1546, 2472, 2538
BsiSI CCGG 7 cut(s) 34, 946, 1918, 1999, 2105, 2404, 2410
BslFI GGGAC 1 cut(s) 2408
BsmAI GTCTC 4 cut(s) 686, 1038, 1623, 1997
BsmBI CGTCTC 2 cut(s) 1623, 1997
BsmFI GGGAC 1 cut(s) 2408
BsmI GAATGC 3 cut(s) 1389, 1859, 2833
Bso31I GGTCTC 1 cut(s) 686
Bsp1286I GDGCHC 6 cut(s) 494, 708, 822, 1546, 2472, 2538
Bsp13I TCCGGA 1 cut(s) 2403
Bsp143I GATC 6 cut(s) 925, 1731, 1757, 2406, 2413, 2509
BspCNI CTCAG 9 cut(s) 580, 904, 1471, 1933, 1969, 2108, 2362, 2677, 2830
BspEI TCCGGA 1 cut(s) 2403
BspFNI CGCG 1 cut(s) 609
BspLI GGNNCC 9 cut(s) 150, 168, 354, 1776, 2062, 2115, 2386, 2408, 2871
BspMAI CTGCAG 2 cut(s) 878, 2620
BspMI ACCTGC 2 cut(s) 754, 2609
BspPI GGATC 4 cut(s) 1765, 2401, 2414, 2421
BspQI GCTCTTC 1 cut(s) 1046
BspT107I GGYRCC 1 cut(s) 2384
BspTNI GGTCTC 1 cut(s) 686
BsrDI GCAATG 2 cut(s) 582, 1747
BsrFI RCCGGY 2 cut(s) 33, 1917
BsrI ACTGG 3 cut(s) 645, 1906, 2673
BssAI RCCGGY 2 cut(s) 33, 1917
BssECI CCNNGG 7 cut(s) 28, 38, 87, 607, 1661, 1997, 2573
BssMI GATC 6 cut(s) 925, 1731, 1757, 2406, 2413, 2509
BssNAI GTATAC 1 cut(s) 2043
BssSI CACGAG 1 cut(s) 493
BssT1I CCWWGG 1 cut(s) 1661
Bst1107I GTATAC 1 cut(s) 2043
Bst2BI CACGAG 1 cut(s) 493
Bst4CI ACNGT 6 cut(s) 710, 998, 1634, 2474, 2518, 2530
BstAPI GCANNNNNTGC 3 cut(s) 630, 873, 1395
BstC8I GCNNGC 8 cut(s) 35, 631, 1058, 1524, 1794, 1919, 2752, 2790
BstDSI CCRYGG 2 cut(s) 607, 2573
BstF5I GGATG 9 cut(s) 649, 763, 1246, 1288, 1719, 1940, 2383, 2436, 2848
BstFNI CGCG 1 cut(s) 609
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 3 cut(s) 59, 845, 2886
BstKTI GATC 6 cut(s) 928, 1734, 1760, 2409, 2416, 2512
BstMAI GTCTC 4 cut(s) 686, 1038, 1623, 1997
BstMBI GATC 6 cut(s) 925, 1731, 1757, 2406, 2413, 2509
BstMCI CGRYCG 1 cut(s) 38
BstNSI RCATGY 4 cut(s) 1526, 1542, 2468, 2792
BstPAI GACNNNNGTC 3 cut(s) 1989, 2112, 2582
BstSCI CCNGG 3 cut(s) 945, 1997, 2409
BstSFI CTRYAG 3 cut(s) 874, 2259, 2616
BstSLI GKGCMC 3 cut(s) 494, 1546, 2472
BstUI CGCG 1 cut(s) 609
BstV2I GAAGAC 2 cut(s) 999, 1094
BstX2I RGATCY 2 cut(s) 2406, 2413
BstXI CCANNNNNNTGG 1 cut(s) 2594
BstYI RGATCY 2 cut(s) 2406, 2413
BstZ17I GTATAC 1 cut(s) 2043
BstZI CGGCCG 1 cut(s) 35
Bsu36I CCTNAGG 2 cut(s) 2349, 2838
BsuI GTATCC 2 cut(s) 548, 570
BtgI CCRYGG 2 cut(s) 607, 2573
BtsCI GGATG 9 cut(s) 649, 763, 1246, 1288, 1719, 1940, 2383, 2436, 2848
BtsI GCAGTG 3 cut(s) 339, 432, 2182
BtsIMutI CAGTG 8 cut(s) 339, 432, 461, 706, 1709, 2182, 2470, 2666
BveI ACCTGC 2 cut(s) 754, 2609
Cac8I GCNNGC 8 cut(s) 35, 631, 1058, 1524, 1794, 1919, 2752, 2790
CaiI CAGNNNCTG 2 cut(s) 1166, 1602
CfoI GCGC 3 cut(s) 59, 845, 2886
Cfr10I RCCGGY 2 cut(s) 33, 1917
Cfr13I GGNCC 5 cut(s) 2114, 2233, 2345, 2748, 2870
Cfr42I CCGCGG 1 cut(s) 610
CseI GACGC 1 cut(s) 195
Csp6I GTAC 1 cut(s) 862
CviQI GTAC 1 cut(s) 862
DpnI GATC 6 cut(s) 927, 1733, 1759, 2408, 2415, 2511
DpnII GATC 6 cut(s) 925, 1731, 1757, 2406, 2413, 2509
EaeI YGGCCR 1 cut(s) 35
EagI CGGCCG 1 cut(s) 35
EciI GGCGGA 4 cut(s) 214, 419, 1956, 2724
Ecl136II GAGCTC 1 cut(s) 820
EclXI CGGCCG 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 1661
Eco24I GRGCYC 1 cut(s) 822
Eco31I GGTCTC 1 cut(s) 686
Eco47I GGWCC 1 cut(s) 2114
Eco52I CGGCCG 1 cut(s) 35
Eco53kI GAGCTC 1 cut(s) 820
Eco57I CTGAAG 4 cut(s) 1319, 1368, 2247, 2385
Eco81I CCTNAGG 2 cut(s) 2349, 2838
EcoICRI GAGCTC 1 cut(s) 820
EcoO109I RGGNCCY 1 cut(s) 2870
EcoRI GAATTC 1 cut(s) 102
EcoT14I CCWWGG 1 cut(s) 1661
EcoT22I ATGCAT 1 cut(s) 1524
EcoT38I GRGCYC 1 cut(s) 822
ErhI CCWWGG 1 cut(s) 1661
Esp3I CGTCTC 2 cut(s) 1623, 1997
FalI AAGNNNNNCTT 6 cut(s) 618, 650, 1031, 1063, 2220, 2252
FaqI GGGAC 1 cut(s) 2408
FauI CCCGC 1 cut(s) 1261
FbaI TGATCA 1 cut(s) 2509
FblI GTMKAC 1 cut(s) 2042
FokI GGATG 9 cut(s) 656, 770, 1253, 1295, 1726, 1947, 2390, 2423, 2855
FriOI GRGCYC 1 cut(s) 822
FseI GGCCGGCC 2 cut(s) 37, 1921
FspBI CTAG 3 cut(s) 671, 1527, 1626
GlaI GCGC 3 cut(s) 58, 844, 2885
GsaI CCCAGC 1 cut(s) 1774
GsuI CTGGAG 4 cut(s) 86, 722, 2447, 2786
HaeII RGCGCY 1 cut(s) 60
HapII CCGG 7 cut(s) 34, 946, 1918, 1999, 2105, 2404, 2410
HgaI GACGC 1 cut(s) 195
HhaI GCGC 3 cut(s) 59, 845, 2886
Hin6I GCGC 3 cut(s) 57, 843, 2884
HinP1I GCGC 3 cut(s) 57, 843, 2884
HincII GTYRAC 1 cut(s) 1585
HindII GTYRAC 1 cut(s) 1585
HindIII AAGCTT 1 cut(s) 2177
HpaII CCGG 7 cut(s) 34, 946, 1918, 1999, 2105, 2404, 2410
HphI GGTGA 9 cut(s) 245, 778, 1733, 1880, 2039, 2647, 2708, 2782, 2834
Hpy166II GTNNAC 9 cut(s) 305, 492, 1179, 1356, 1544, 1585, 2043, 2470, 2577
Hpy188III TCNNGA 9 cut(s) 65, 671, 911, 956, 1172, 1208, 1626, 2351, 2404
Hpy8I GTNNAC 9 cut(s) 305, 492, 1179, 1356, 1544, 1585, 2043, 2470, 2577
Hpy99I CGWCG 1 cut(s) 689
HpyAV CCTTC 8 cut(s) 76, 212, 424, 644, 826, 1117, 1907, 2803
HpyCH4III ACNGT 6 cut(s) 710, 998, 1634, 2474, 2518, 2530
HpyCH4IV ACGT 1 cut(s) 435
HpySE526I ACGT 1 cut(s) 435
HspAI GCGC 3 cut(s) 57, 843, 2884
Kpn2I TCCGGA 1 cut(s) 2403
KroI GCCGGC 2 cut(s) 33, 1917
KroNI GCCGGC 2 cut(s) 35, 1919
Ksp22I TGATCA 1 cut(s) 2509
KspI CCGCGG 1 cut(s) 610
Kzo9I GATC 6 cut(s) 925, 1731, 1757, 2406, 2413, 2509
LguI GCTCTTC 1 cut(s) 1046
LmnI GCTCC 7 cut(s) 116, 148, 172, 352, 703, 2149, 2533
LweI GCATC 3 cut(s) 1567, 2188, 2590
MaeI CTAG 3 cut(s) 671, 1527, 1626
MaeII ACGT 1 cut(s) 435
MaeIII GTNAC 4 cut(s) 1508, 2170, 2318, 2491
MalI GATC 6 cut(s) 927, 1733, 1759, 2408, 2415, 2511
MboI GATC 6 cut(s) 925, 1731, 1757, 2406, 2413, 2509
MflI RGATCY 2 cut(s) 2406, 2413
MhlI GDGCHC 6 cut(s) 494, 708, 822, 1546, 2472, 2538
MlyI GAGTC 5 cut(s) 170, 1148, 1184, 1343, 1361
MmeI TCCRAC 1 cut(s) 1648
Mph1103I ATGCAT 1 cut(s) 1524
MroI TCCGGA 1 cut(s) 2403
MroNI GCCGGC 2 cut(s) 33, 1917
MroXI GAANNNNTTC 1 cut(s) 2721
MseI TTAA 5 cut(s) 803, 1263, 1563, 1610, 2205
MslI CAYNNNNRTG 3 cut(s) 1363, 1549, 2451
MspA1I CMGCKG 8 cut(s) 358, 398, 609, 730, 1602, 2225, 2363, 2425
MspI CCGG 7 cut(s) 34, 946, 1918, 1999, 2105, 2404, 2410
MspR9I CCNGG 3 cut(s) 947, 1999, 2411
Mva1269I GAATGC 3 cut(s) 1389, 1859, 2833
MvnI CGCG 1 cut(s) 609
NaeI GCCGGC 2 cut(s) 35, 1919
NciI CCSGG 3 cut(s) 947, 1999, 2411
NdeII GATC 6 cut(s) 925, 1731, 1757, 2406, 2413, 2509
NgoMIV GCCGGC 2 cut(s) 33, 1917
NlaIV GGNNCC 9 cut(s) 150, 168, 354, 1776, 2062, 2115, 2386, 2408, 2871
NmeAIII GCCGAG 3 cut(s) 53, 63, 112
NmuCI GTSAC 1 cut(s) 2170
NsiI ATGCAT 1 cut(s) 1524
NspI RCATGY 4 cut(s) 1526, 1542, 2468, 2792
OliI CACNNNNGTG 1 cut(s) 1549
PaeI GCATGC 2 cut(s) 1526, 2792
PaqCI CACCTGC 1 cut(s) 754
PciI ACATGT 1 cut(s) 1538
PciSI GCTCTTC 1 cut(s) 1046
PcsI WCGNNNNNNNCGW 1 cut(s) 500
PctI GAATGC 3 cut(s) 1389, 1859, 2833
PdiI GCCGGC 2 cut(s) 35, 1919
PdmI GAANNNNTTC 1 cut(s) 2721
PfoI TCCNGGA 1 cut(s) 2409
PleI GAGTC 5 cut(s) 169, 1148, 1183, 1342, 1360
PpsI GAGTC 5 cut(s) 169, 1148, 1183, 1342, 1360
PscI ACATGT 1 cut(s) 1538
PshAI GACNNNNGTC 3 cut(s) 1989, 2112, 2582
Psp124BI GAGCTC 1 cut(s) 822
PspFI CCCAGC 1 cut(s) 1770
PspN4I GGNNCC 9 cut(s) 150, 168, 354, 1776, 2062, 2115, 2386, 2408, 2871
PspPI GGNCC 5 cut(s) 2114, 2233, 2345, 2748, 2870
PstI CTGCAG 2 cut(s) 878, 2620
PstNI CAGNNNCTG 2 cut(s) 1166, 1602
PsuI RGATCY 2 cut(s) 2406, 2413
PvuII CAGCTG 5 cut(s) 730, 1602, 2225, 2363, 2425
RigI GGCCGGCC 2 cut(s) 37, 1921
RsaI GTAC 1 cut(s) 863
RsaNI GTAC 1 cut(s) 862
RseI CAYNNNNRTG 3 cut(s) 1363, 1549, 2451
SacI GAGCTC 1 cut(s) 822
SacII CCGCGG 1 cut(s) 610
SapI GCTCTTC 1 cut(s) 1046
SaqAI TTAA 5 cut(s) 803, 1263, 1563, 1610, 2205
Sau3AI GATC 6 cut(s) 925, 1731, 1757, 2406, 2413, 2509
Sau96I GGNCC 5 cut(s) 2114, 2233, 2345, 2748, 2870
SbfI CCTGCAGG 1 cut(s) 2620
SchI GAGTC 5 cut(s) 170, 1148, 1184, 1343, 1361
ScrFI CCNGG 3 cut(s) 947, 1999, 2411
SdaI CCTGCAGG 1 cut(s) 2620
SduI GDGCHC 6 cut(s) 494, 708, 822, 1546, 2472, 2538
SfaNI GCATC 3 cut(s) 1567, 2188, 2590
SfcI CTRYAG 3 cut(s) 874, 2259, 2616
Sfr303I CCGCGG 1 cut(s) 610
SgrBI CCGCGG 1 cut(s) 610
SinI GGWCC 1 cut(s) 2114
SmiMI CAYNNNNRTG 3 cut(s) 1363, 1549, 2451
SmlI CTYRAG 6 cut(s) 821, 1170, 1588, 1817, 1949, 2549
SmoI CTYRAG 6 cut(s) 821, 1170, 1588, 1817, 1949, 2549
SphI GCATGC 2 cut(s) 1526, 2792
Sse8387I CCTGCAGG 1 cut(s) 2620
SspI AATATT 2 cut(s) 1204, 2209
SspMI CTAG 3 cut(s) 671, 1527, 1626
SstI GAGCTC 1 cut(s) 822
StyD4I CCNGG 3 cut(s) 945, 1997, 2409
StyI CCWWGG 1 cut(s) 1661
TaaI ACNGT 6 cut(s) 710, 998, 1634, 2474, 2518, 2530
TaiI ACGT 1 cut(s) 438
TaqI TCGA 6 cut(s) 637, 684, 884, 955, 1011, 2568
TatI WGTACW 1 cut(s) 861
TauI GCSGC 8 cut(s) 8, 199, 317, 358, 401, 404, 609, 612
Tru1I TTAA 5 cut(s) 803, 1263, 1563, 1610, 2205
Tru9I TTAA 5 cut(s) 803, 1263, 1563, 1610, 2205
TscAI CASTG 8 cut(s) 339, 432, 461, 713, 1709, 2189, 2477, 2673
TseFI GTSAC 1 cut(s) 2170
Tsp45I GTSAC 1 cut(s) 2170
TspGWI ACGGA 2 cut(s) 726, 2729
TspRI CASTG 8 cut(s) 339, 432, 461, 713, 1709, 2189, 2477, 2673
VneI GTGCAC 3 cut(s) 490, 1542, 2468
VpaK11BI GGWCC 1 cut(s) 2114
XapI RAATTY 5 cut(s) 102, 1256, 2012, 2456, 2558
XbaI TCTAGA 2 cut(s) 670, 1625
XceI RCATGY 4 cut(s) 1526, 1542, 2468, 2792
XcmI CCANNNNNNNNNTGG 3 cut(s) 1785, 1855, 1898
XmiI GTMKAC 1 cut(s) 2042
XmnI GAANNNNTTC 1 cut(s) 2721
XspI CTAG 3 cut(s) 671, 1527, 1626
Zsp2I ATGCAT 1 cut(s) 1524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.