RchiOBHm_Chr7g0224041

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000280
Physical Location & Seq
Forward (+)
45904465 .. 45909326
4862 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20060

Sequence Viewer

Length: 4542 bp
ATGAAATTCAAGGCGACGGCGGCGAGATTGAGGCCCGGAGGCACAAAGCGACGGAACGGCGGTGGTGTGGGTATTTTGGGAATTCAGAACGACGACGGAACAAATGGTGGAGGACCGTCTGTGGAGCCAAAGCTTGTTCCCTGGGGGAGGAAGCGGCGGAGGACAGGATGCGAGGGTATTTTGGGTTATCAAAATGAAGGAAAGGCAAGGGCTGTGAAGGCGAAGAAGAAGAAGAAGCAGCAGCAGCAGCAGCAGAGAAGGGCTCCCTTGAGGGAGGTGACAATATGCCATCAATGTAGGAAAACAGGTACGGGAAGAACGGTGCGGTGCACATGGTGTTCGAGGAACAAGTATTGTATCCCTTGCATACAGAGGTGGTATCCCAACACATCAGAGGAAGCCATAGCTGAGGCTTGTCCTGTGTGCCGCGGCAACTGCAACTGCTATACCTGCCTTCGGTTGAATGTGCCTAATAGGTGTTTGAAGAATCTGGACCCGAAAATCGATGAGGAGACCAAGCTGGAGCACTGTAAGTATATGGTTCATCAGCTGCTTCCGTATTTGAAGAGGATTAGGGACGAGCAGGAGAGTGAGATGGTGGTCGAGGCTAATAGGCTGGGTTTAGGTGATGTTTCGGAGCTCAAGGTGGACAAGGCTAATTGCGGTGAAGACGAGCGTATGTACTGCAACAACTGCAGCACTTCGATTTTTGATTTTCATAGGACTTGTCCTGAGTGTGAGTATGATCTGTGCCTCGGTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAACAAGAGAGCGGAAGTGTCCACACACATATTCTGGAGAACAAGTGAAGATGGAAGCATTCTTTGCCCCCCAACACACATGAAGGGTTGTGGTAAGCATAATTTAGAATTGAGATGCCTTTTTCCTCAGAATCATGTCATGGAATTGGTAGAGAACGCAGGAAAAATAGATGCAAGTTACAATTTTTTGCCTGATCGTAGTGAAACATGTGCATACAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTTGCTCAAGTAGTAGCAGCAGCTCTAAGTTACGGAAAGCAGCTTCTAGAGTTGCTTCTGATGATAACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAGGATAAGGATTTTGAGCATTTTCAGTGGCATTGGAAGAGGGGTGAGCCTGTGATTGTTAGCAATGCACTTGAAAATGGATCAGGTTTAAGCTGGGAACCATTTGTCATGTGGCGGGCATTTAAGCAGACAAATAATATCAACCATGCTAGTGAATCGATATTCCACGCCATTGATTGCTGGTATTGGTCTTTTATAGAAATCCACATCCTCCATTTCTGTAGTGGATATTCCAAGGGACGGTCGTATAAGACAGGATGGCCTCAGAATCTCAAGCTCACTGACTTCCCATCATCTACTGAATTTCACAATCGTCTTCCCCGGCATGGTGAGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATAGTCACCCCAACAAGGGAACTCTGAACCTTGCTATCTACTTGCCACCTATCTCAGTAAAGCCGGACATGGGTCCAAAAACGTACCTTGCCTATGGTTTTGCAGAGGAGCTTGGATGGGGAGATTCTGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTTAATATTCTGACACATACAACTGAAGTGGCCCTTACAACTGAAGAACTTGCAACTATAGATAAGTTGAAGAAAAAGCTGATGGAGCAAGACGAAAGGGAGATATTTGGGAATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCTCAGCAAGAGTCAGCTATAGAGAAGTTGAAGAGAAAGTATAGGGAGCAAAACGAAAGAGAGATATTTGGGAATTGTCATACAAGTGCTGGAAATGGAAGTGGGCCTCAGCAAGAGTCAGCTGAAATATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGATCCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTATGGATGTTCTCTGTAATCCTTTGCAGCATGTTACTCATGCTATACACGATCAGACTGTCTATCTCACTGTGGAACACAAGAGGAAGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTGGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCAACAAACCACAGAGCCCATGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTATGAAGGAGATTCCTGGTGAGAATGTGACAAAGTGTGCAAGGGTTCGCTTGGACGAGTTGAGACGTTGCCATCAGTGTCATGTGAATGTTATGGGAAGAGTGGTGCAGTGCACAAGGTGTCGGGAAAGAGCTACGGGAAGGAGGACAAGTTATTGTATCCCTTGTATACAGAGATGGTATCCCAACACATCAGAGGAAGCCATTGCTAAGGCTTGTCCGTTATGCTGCGGCAACTGCAACTGCACAGCCTGTCTTCGCTTGGATGTGCCTGTCAGGTGTTTGCAGAATCTAGAGCCAAAAATCGACAAGGGTACCAAGCTGGAGCACTCTAAGTATTTGGTCCATCAGCTGCTTCCCTATTTGAAGATGATTAGGGATGAGCAGGTGAGTGAGATGCCGGTGGAAGCCAAGAGGCAGGGCTTACATGACCTTTCGGAGCTCAAGGTGGAGAAGGCAAAAAGCGGTGATGGAGTGCGTATGTACTGCAACAGCTGCAGCACTTCGATTTTTGATTTTCATAGGACTTGTCCCGGATGTGAGTATGATCTGTGCCTCGGTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAAGAAGAGAGCTCAAGCGTCCACATTTGAGTGGAAATGGAGTGCAAGTGAAGATGGAAGCATTCTCTGTCCCCCAACACACATGAAAGGTTGTGGTAAGCACAATTTAGAATTGAGATGCCTTTTTTCTCAGAATCATGTCATGGAATTGGTAGAGAAAGCAGGAGAAATAGATGCAAGTTACAATTTTTTGCCTGATCGTAGTGAAACATGTGCATATAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTTAAGTAGTAGCAGCTGCTGTAAGTTAAGGAAAGCAGCTTCTAGAGTTGCTTCTGATAGTAACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAAGATAAGGATTTTGAGCATTTTCAGTGGCATTGGAAGAGGGGTGAGCCTGTGATCGTTAGCAGTGCGCTTGAAAATGGATCAGGTTTAAGCTGGGAACCATATGTCATGTGGCAGGCATTTAGAAAGACAAATAATATCAACCATGCTAGTGAATCGACAGTCCATGCCATTGATTGCTGGGATTGGTGTTTTATAGAAATAAACATCCACCATTTCTTTTGTGGATATTCCAAAGGACGGTCTTATAAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATCATCTACTGAGTTTCACAATCGTCTTCCCCGGCATGGTAGGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATACTCACCCCAACAAGGGAACTCTCAACCTTGCTATCAACTTGCCACCTATCTCAGTAAAGCCAGACATGGGTCCAAAAGCATATATTGCCTATGGTTTTGCACAGGAGCTTGGACGGGGAGATTCTGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTTAATATTCTGACGCATACAGCTGAAGTGGCCCTTACAACTGAGCAACTTGTAACTGTGGAGAAGTTGAAGAAAAAGCACAGGGAGCAAGACAAAAGAGAGATATTTGGGAATTGTCACTCAAGTGCTGGAAATGGAAGTGGGTCTCAGGAAGAATTAGCTGATGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGACTCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTAAGCATGTGTTCTGTAATCCTTTGCAGCATGTTACTCATGCTATACATGATCAGACTGTTTATCTCACTGTGGAGCACAAGAGGAAGCTGAAGGAGGAATTCGGTATCGAGCCTTGGACATTTGTCCAAAAGCTTGGAGATGCTGTTTTCATTCCTGCTGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTGGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACATACCACAGAGCCCATGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTGTGAAGGAGATTTCTGGTGAGAATGTGATGACTGAGGATGGGAAGTCTAGGGGAGGCGCAAGGGAATTAAGGCAAGGTGTAAGGCGCAAGTCTTACAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1513

Amino Acids

172.39

Weight (kDa)

8.3

Isoelectric Point (pI)

48.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 92 - 154 1.2e-06 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 660 - 747 5.4e-13 JmjC domain, hydroxylase
JmjC PF02373 1339 - 1436 1.3e-13 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3558
AarI CACCTGC 1 cut(s) 2743
Acc36I ACCTGC 3 cut(s) 458, 2174, 2743
Acc65I GGTACC 1 cut(s) 2681
AccB1I GGYRCC 3 cut(s) 1949, 2681, 4016
AccB7I CCANNNNNTGG 2 cut(s) 1127, 3299
AccBSI CCGCTC 1 cut(s) 800
AccI GTMKAC 2 cut(s) 2536, 3674
AccII CGCG 1 cut(s) 429
AccIII TCCGGA 2 cut(s) 1968, 4035
AclWI GGATC 4 cut(s) 1225, 1966, 1979, 3397
AcsI RAATTY 9 cut(s) 5, 81, 1439, 1472, 2021, 2123, 3644, 4088, 4190
AcuI CTGAAG 4 cut(s) 1707, 1725, 3879, 4202
AdeI CACNNNGTG 2 cut(s) 336, 2487
AfaI GTAC 5 cut(s) 310, 683, 1586, 2683, 2852
AflII CTTAAG 1 cut(s) 3217
AflIII ACRYGT 2 cut(s) 995, 3167
AjiI CACGTC 2 cut(s) 2014, 4081
AjnI CCWGG 4 cut(s) 140, 1137, 1974, 2383
AleI CACNNNNGTG 2 cut(s) 1785, 3957
Alw21I GWGCWC 8 cut(s) 332, 528, 642, 2483, 2697, 2811, 2971, 4170
Alw26I GTCTC 3 cut(s) 506, 2425, 3984
Alw44I GTGCAC 2 cut(s) 328, 2479
AlwI GGATC 4 cut(s) 1225, 1966, 1979, 3397
AlwNI CAGNNNCTG 1 cut(s) 3234
Aor13HI TCCGGA 2 cut(s) 1968, 4035
AoxI GGCC 7 cut(s) 32, 1397, 1692, 1805, 1910, 3569, 3864
ApaLI GTGCAC 2 cut(s) 328, 2479
ApoI RAATTY 9 cut(s) 5, 81, 1439, 1472, 2021, 2123, 3644, 4088, 4190
Asp700I GAANNNNTTC 2 cut(s) 2286, 4353
Asp718I GGTACC 1 cut(s) 2681
AspLEI GCGC 3 cut(s) 3379, 4496, 4524
AsuC2I CCSGG 6 cut(s) 36, 767, 1459, 2901, 2936, 3631
AvaII GGWCC 5 cut(s) 113, 493, 1574, 2710, 3746
BaeGI GKGCMC 2 cut(s) 332, 2483
BaeI ACNNNNGTAYC 2 cut(s) 2665, 2698
BamHI GGATCC 1 cut(s) 1971
BanI GGYRCC 3 cut(s) 1949, 2681, 4016
BanII GRGCYC 6 cut(s) 265, 642, 2317, 2811, 2971, 4384
BarI GAAGNNNNNNTAC 4 cut(s) 1623, 1655, 3795, 3827
BbsI GAAGAC 4 cut(s) 675, 1445, 2615, 3617
Bbv12I GWGCWC 8 cut(s) 332, 528, 642, 2483, 2697, 2811, 2971, 4170
BbvCI CCTCAGC 3 cut(s) 408, 1809, 1914
BceAI ACGGC 5 cut(s) 33, 73, 1022, 2122, 3194
BciT130I CCWGG 4 cut(s) 142, 1139, 1976, 2385
BciVI GTATCC 4 cut(s) 368, 390, 2537, 2559
BclI TGATCA 1 cut(s) 4141
BcnI CCSGG 6 cut(s) 36, 767, 1459, 2901, 2936, 3631
BcoDI GTCTC 3 cut(s) 506, 2425, 3984
BfaI CTAG 6 cut(s) 1086, 1287, 2660, 3258, 3459, 4485
BfmI CTRYAG 6 cut(s) 694, 1357, 1719, 1824, 2181, 2863
BfrI CTTAAG 1 cut(s) 3217
BfuAI ACCTGC 3 cut(s) 458, 2174, 2743
BfuI GTATCC 4 cut(s) 368, 390, 2537, 2559
Bme18I GGWCC 5 cut(s) 113, 493, 1574, 2710, 3746
BmgBI CACGTC 2 cut(s) 2014, 4081
BoxI GACNNNNGTC 4 cut(s) 1572, 2147, 3744, 4214
BpiI GAAGAC 4 cut(s) 675, 1445, 2615, 3617
BplI GAGNNNNNCTC 2 cut(s) 247, 279
BpmI CTGGAG 7 cut(s) 542, 844, 2012, 2351, 2711, 4079, 4418
Bpu10I CCTNAGC 4 cut(s) 408, 1809, 1914, 2577
BpuEI CTTGAG 9 cut(s) 289, 626, 1030, 1394, 2099, 2795, 2955, 3566, 3940
BpuMI CCSGG 6 cut(s) 36, 767, 1459, 2901, 2936, 3631
Bsa29I ATCGAT 2 cut(s) 504, 1295
BsaI GGTCTC 2 cut(s) 506, 3984
BsaWI WCCGGW 2 cut(s) 1968, 4035
Bse118I RCCGGY 1 cut(s) 2767
Bse1I ACTGG 2 cut(s) 2238, 4305
Bse3DI GCAATG 2 cut(s) 1207, 2571
BseAI TCCGGA 2 cut(s) 1968, 4035
BseBI CCWGG 4 cut(s) 142, 1139, 1976, 2385
BseCI ATCGAT 2 cut(s) 504, 1295
BseMI GCAATG 2 cut(s) 1207, 2571
BseNI ACTGG 2 cut(s) 2238, 4305
BseRI GAGGAG 2 cut(s) 524, 1622
BseSI GKGCMC 2 cut(s) 332, 2483
BseYI CCCAGC 4 cut(s) 616, 1230, 3402, 3489
BsgI GTGCAG 2 cut(s) 2495, 2596
Bsh1236I CGCG 1 cut(s) 429
Bsh1285I CGRYCG 1 cut(s) 1382
BshFI GGCC 7 cut(s) 34, 1399, 1694, 1807, 1912, 3571, 3866
BshNI GGYRCC 3 cut(s) 1949, 2681, 4016
BshVI ATCGAT 2 cut(s) 504, 1295
BsiEI CGRYCG 1 cut(s) 1382
BsiHKAI GWGCWC 8 cut(s) 332, 528, 642, 2483, 2697, 2811, 2971, 4170
BslFI GGGAC 6 cut(s) 590, 1389, 1973, 2883, 3012, 4040
BsmAI GTCTC 3 cut(s) 506, 2425, 3984
BsmBI CGTCTC 1 cut(s) 2425
BsmFI GGGAC 6 cut(s) 590, 1389, 1973, 2883, 3012, 4040
BsmI GAATGC 2 cut(s) 846, 3018
BsnI GGCC 7 cut(s) 34, 1399, 1694, 1807, 1912, 3571, 3866
Bso31I GGTCTC 2 cut(s) 506, 3984
Bsp13I TCCGGA 2 cut(s) 1968, 4035
BspANI GGCC 7 cut(s) 34, 1399, 1694, 1807, 1912, 3571, 3866
BspDI ATCGAT 2 cut(s) 504, 1295
BspEI TCCGGA 2 cut(s) 1968, 4035
BspFNI CGCG 1 cut(s) 429
BspMAI CTGCAG 3 cut(s) 698, 2185, 2867
BspMI ACCTGC 3 cut(s) 458, 2174, 2743
BspPI GGATC 4 cut(s) 1225, 1966, 1979, 3397
BspT107I GGYRCC 3 cut(s) 1949, 2681, 4016
BspTI CTTAAG 1 cut(s) 3217
BspTNI GGTCTC 2 cut(s) 506, 3984
BsrBI CCGCTC 1 cut(s) 800
BsrDI GCAATG 2 cut(s) 1207, 2571
BsrFI RCCGGY 1 cut(s) 2767
BsrI ACTGG 2 cut(s) 2238, 4305
BssAI RCCGGY 1 cut(s) 2767
BssNAI GTATAC 2 cut(s) 2537, 3675
BssT1I CCWWGG 4 cut(s) 1121, 1371, 3293, 4205
Bst1107I GTATAC 2 cut(s) 2537, 3675
Bst2UI CCWGG 4 cut(s) 142, 1139, 1976, 2385
Bst6I CTCTTC 8 cut(s) 560, 1169, 1832, 2277, 2461, 2957, 3341, 4344
BstAFI CTTAAG 1 cut(s) 3217
BstAPI GCANNNNNTGC 4 cut(s) 693, 852, 2862, 3761
BstC8I GCNNGC 4 cut(s) 1254, 2355, 3426, 4422
BstDSI CCRYGG 2 cut(s) 427, 2138
BstENI CCTNNNNNAGG 1 cut(s) 145
BstFNI CGCG 1 cut(s) 429
BstHHI GCGC 3 cut(s) 3379, 4496, 4524
BstMAI GTCTC 3 cut(s) 506, 2425, 3984
BstMCI CGRYCG 1 cut(s) 1382
BstNI CCWGG 4 cut(s) 142, 1139, 1976, 2385
BstNSI RCATGY 7 cut(s) 999, 2057, 2357, 3171, 4100, 4124, 4424
BstPAI GACNNNNGTC 4 cut(s) 1572, 2147, 3744, 4214
BstSFI CTRYAG 6 cut(s) 694, 1357, 1719, 1824, 2181, 2863
BstSLI GKGCMC 2 cut(s) 332, 2483
BstUI CGCG 1 cut(s) 429
BstV2I GAAGAC 4 cut(s) 675, 1445, 2615, 3617
BstX2I RGATCY 1 cut(s) 1971
BstXI CCANNNNNNTGG 2 cut(s) 2159, 4226
BstYI RGATCY 1 cut(s) 1971
BstZ17I GTATAC 2 cut(s) 2537, 3675
Bsu15I ATCGAT 2 cut(s) 504, 1295
BsuI GTATCC 4 cut(s) 368, 390, 2537, 2559
BsuRI GGCC 7 cut(s) 34, 1399, 1694, 1807, 1912, 3571, 3866
BsuTUI ATCGAT 2 cut(s) 504, 1295
BtgI CCRYGG 2 cut(s) 427, 2138
BtrI CACGTC 2 cut(s) 2014, 4081
BtsI GCAGTG 4 cut(s) 1642, 2483, 3379, 3814
BveI ACCTGC 3 cut(s) 458, 2174, 2743
Cac8I GCNNGC 4 cut(s) 1254, 2355, 3426, 4422
CaiI CAGNNNCTG 1 cut(s) 3234
CfoI GCGC 3 cut(s) 3379, 4496, 4524
Cfr10I RCCGGY 1 cut(s) 2767
Cfr42I CCGCGG 1 cut(s) 430
ClaI ATCGAT 2 cut(s) 504, 1295
CseI GACGC 2 cut(s) 2964, 3856
Csp6I GTAC 5 cut(s) 309, 682, 1585, 2682, 2851
CspCI CAANNNNNGTGG 2 cut(s) 1671, 1706
CviQI GTAC 5 cut(s) 309, 682, 1585, 2682, 2851
DraIII CACNNNGTG 2 cut(s) 336, 2487
Eam1104I CTCTTC 8 cut(s) 560, 1169, 1832, 2277, 2461, 2957, 3341, 4344
EarI CTCTTC 8 cut(s) 560, 1169, 1832, 2277, 2461, 2957, 3341, 4344
EciI GGCGGA 1 cut(s) 172
Ecl136II GAGCTC 3 cut(s) 640, 2809, 2969
Eco130I CCWWGG 4 cut(s) 1121, 1371, 3293, 4205
Eco24I GRGCYC 6 cut(s) 265, 642, 2317, 2811, 2971, 4384
Eco31I GGTCTC 2 cut(s) 506, 3984
Eco47I GGWCC 5 cut(s) 113, 493, 1574, 2710, 3746
Eco53kI GAGCTC 3 cut(s) 640, 2809, 2969
Eco57I CTGAAG 4 cut(s) 1707, 1725, 3879, 4202
EcoICRI GAGCTC 3 cut(s) 640, 2809, 2969
EcoNI CCTNNNNNAGG 1 cut(s) 145
EcoRI GAATTC 3 cut(s) 81, 2123, 4190
EcoRII CCWGG 4 cut(s) 140, 1137, 1974, 2383
EcoT14I CCWWGG 4 cut(s) 1121, 1371, 3293, 4205
EcoT38I GRGCYC 6 cut(s) 265, 642, 2317, 2811, 2971, 4384
ErhI CCWWGG 4 cut(s) 1121, 1371, 3293, 4205
Esp3I CGTCTC 1 cut(s) 2425
FalI AAGNNNNNCTT 4 cut(s) 1680, 1712, 3852, 3884
FaqI GGGAC 6 cut(s) 590, 1389, 1973, 2883, 3012, 4040
FauI CCCGC 1 cut(s) 1245
FauNDI CATATG 1 cut(s) 3412
FbaI TGATCA 1 cut(s) 4141
FblI GTMKAC 2 cut(s) 2536, 3674
FriOI GRGCYC 6 cut(s) 265, 642, 2317, 2811, 2971, 4384
FspBI CTAG 6 cut(s) 1086, 1287, 2660, 3258, 3459, 4485
GlaI GCGC 3 cut(s) 3378, 4495, 4523
GsaI CCCAGC 4 cut(s) 620, 1234, 3406, 3493
GsuI CTGGAG 7 cut(s) 542, 844, 2012, 2351, 2711, 4079, 4418
HaeIII GGCC 7 cut(s) 34, 1399, 1694, 1807, 1912, 3571, 3866
HgaI GACGC 2 cut(s) 2964, 3856
HhaI GCGC 3 cut(s) 3379, 4496, 4524
Hin6I GCGC 3 cut(s) 3377, 4494, 4522
HinP1I GCGC 3 cut(s) 3377, 4494, 4522
HindIII AAGCTT 4 cut(s) 131, 1637, 3809, 4223
Hpy166II GTNNAC 8 cut(s) 330, 649, 810, 2142, 2481, 2537, 2979, 3675
Hpy8I GTNNAC 8 cut(s) 330, 649, 810, 2142, 2481, 2537, 2979, 3675
Hpy99I CGWCG 4 cut(s) 19, 54, 95, 98
HpyCH4IV ACGT 4 cut(s) 1583, 2013, 2434, 4080
HpySE526I ACGT 4 cut(s) 1583, 2013, 2434, 4080
HspAI GCGC 3 cut(s) 3377, 4494, 4522
Kpn2I TCCGGA 2 cut(s) 1968, 4035
KpnI GGTACC 1 cut(s) 2685
Ksp22I TGATCA 1 cut(s) 4141
KspI CCGCGG 1 cut(s) 430
MaeI CTAG 6 cut(s) 1086, 1287, 2660, 3258, 3459, 4485
MaeII ACGT 4 cut(s) 1583, 2013, 2434, 4080
MbiI CCGCTC 1 cut(s) 800
MflI RGATCY 1 cut(s) 1971
MlyI GAGTC 3 cut(s) 1826, 1931, 4033
MmeI TCCRAC 4 cut(s) 1108, 2324, 3280, 4391
MroI TCCGGA 2 cut(s) 1968, 4035
MroXI GAANNNNTTC 2 cut(s) 2286, 4353
MspA1I CMGCKG 9 cut(s) 429, 550, 1928, 1990, 2719, 2862, 3231, 3857, 4057
MspCI CTTAAG 1 cut(s) 3217
Mva1269I GAATGC 2 cut(s) 846, 3018
MvaI CCWGG 4 cut(s) 142, 1139, 1976, 2385
MvnI CGCG 1 cut(s) 429
NciI CCSGG 6 cut(s) 36, 767, 1459, 2901, 2936, 3631
NdeI CATATG 1 cut(s) 3412
NmuCI GTSAC 5 cut(s) 277, 1505, 1778, 2395, 3950
NspI RCATGY 7 cut(s) 999, 2057, 2357, 3171, 4100, 4124, 4424
OliI CACNNNNGTG 2 cut(s) 1785, 3957
PaeI GCATGC 2 cut(s) 2357, 4424
PaqCI CACCTGC 1 cut(s) 2743
PasI CCCWGGG 1 cut(s) 141
PciI ACATGT 2 cut(s) 995, 3167
PctI GAATGC 2 cut(s) 846, 3018
PdmI GAANNNNTTC 2 cut(s) 2286, 4353
PflMI CCANNNNNTGG 2 cut(s) 1127, 3299
PfoI TCCNGGA 3 cut(s) 1137, 1974, 2899
PleI GAGTC 3 cut(s) 1825, 1930, 4033
PpsI GAGTC 3 cut(s) 1825, 1930, 4033
PscI ACATGT 2 cut(s) 995, 3167
PshAI GACNNNNGTC 4 cut(s) 1572, 2147, 3744, 4214
PsiI TTATAA 1 cut(s) 3558
Psp124BI GAGCTC 3 cut(s) 642, 2811, 2971
Psp6I CCWGG 4 cut(s) 140, 1137, 1974, 2383
PspFI CCCAGC 4 cut(s) 616, 1230, 3402, 3489
PspGI CCWGG 4 cut(s) 140, 1137, 1974, 2383
PstI CTGCAG 3 cut(s) 698, 2185, 2867
PstNI CAGNNNCTG 1 cut(s) 3234
PsuI RGATCY 1 cut(s) 1971
PvuII CAGCTG 8 cut(s) 550, 1928, 1990, 2719, 2862, 3231, 3857, 4057
RsaI GTAC 5 cut(s) 310, 683, 1586, 2683, 2852
RsaNI GTAC 5 cut(s) 309, 682, 1585, 2682, 2851
SacI GAGCTC 3 cut(s) 642, 2811, 2971
SacII CCGCGG 1 cut(s) 430
SbfI CCTGCAGG 1 cut(s) 2185
SchI GAGTC 3 cut(s) 1826, 1931, 4033
SdaI CCTGCAGG 1 cut(s) 2185
SfcI CTRYAG 6 cut(s) 694, 1357, 1719, 1824, 2181, 2863
Sfr303I CCGCGG 1 cut(s) 430
SgrBI CCGCGG 1 cut(s) 430
SinI GGWCC 5 cut(s) 113, 493, 1574, 2710, 3746
SphI GCATGC 2 cut(s) 2357, 4424
Sse8387I CCTGCAGG 1 cut(s) 2185
SspI AATATT 2 cut(s) 1669, 3841
SspMI CTAG 6 cut(s) 1086, 1287, 2660, 3258, 3459, 4485
SstI GAGCTC 3 cut(s) 642, 2811, 2971
StyI CCWWGG 4 cut(s) 1121, 1371, 3293, 4205
TaiI ACGT 4 cut(s) 1586, 2016, 2437, 4083
TatI WGTACW 2 cut(s) 681, 2850
TauI GCSGC 5 cut(s) 23, 157, 429, 432, 2601
TseFI GTSAC 5 cut(s) 277, 1505, 1778, 2395, 3950
Tsp45I GTSAC 5 cut(s) 277, 1505, 1778, 2395, 3950
TspGWI ACGGA 7 cut(s) 67, 111, 546, 1087, 2294, 2577, 4361
Van91I CCANNNNNTGG 2 cut(s) 1127, 3299
Vha464I CTTAAG 1 cut(s) 3217
VneI GTGCAC 2 cut(s) 328, 2479
VpaK11BI GGWCC 5 cut(s) 113, 493, 1574, 2710, 3746
XagI CCTNNNNNAGG 1 cut(s) 145
XapI RAATTY 9 cut(s) 5, 81, 1439, 1472, 2021, 2123, 3644, 4088, 4190
XbaI TCTAGA 3 cut(s) 1085, 2659, 3257
XceI RCATGY 7 cut(s) 999, 2057, 2357, 3171, 4100, 4124, 4424
XcmI CCANNNNNNNNNTGG 4 cut(s) 1245, 1358, 3417, 3530
XmiI GTMKAC 2 cut(s) 2536, 3674
XmnI GAANNNNTTC 2 cut(s) 2286, 4353
XspI CTAG 6 cut(s) 1086, 1287, 2660, 3258, 3459, 4485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.