Rh7DG359600

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000280
Physical Location & Seq
Forward (+)
47902957 .. 47907120
4164 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG359600.1

Sequence Viewer

Length: 4164 bp
ATGAGAATCCAGGCGACGCCGTCGAGATTGTGGCCCCGAGGCACAAAGCGGCCGAACATATGGGGAATTCAGAACGACGACGGAACAAATGGGGAAGAAGATGGTGGAGGATCGTCTGTGGAGCCACTGCCAAAGCTTGTTCCCTGGGGGAGGAAGCGGCGGAGGCTAGGATGCGAGGGTTTTTGGGCAAATCAAAATGAAGGAAACGCAAGGGCTGTGAAGGCGAATAAGAAGAAGAAGAAGCAGCAGCAGCAGCAGCAGCAGCAGAGAAGGGCTCCCTTGGAGGTGCAGCAGCAGACAAGGGATAAGCAAGTACCACTACCTAGCTACCTGCAGAGAAGGGCTCCCTTGGAGGTGCAGCAGCAGAGTAGGGATAAACAAGAACCACTACCTAGCTACCTGCAGAGAAGGACTCCCTTGAAGGTACGGACAATCAATTGCCATCAATGTAAGCAAAATGATATGGGAAGAGCGGTGCGGTGCACAAAGTGTTCGAGGAAGAGGTATTGTATCCCTTGCATAAGGACTTGGTATCCCAACACCTCAGAGGAAGCCATAGCTGAGGCTTGTCCTGCGTGCCGCGGCAACTGCAACTGCAATGTCTGCCTTTGTTGGGGTGTGCCTGTCAGGAATCTGAACCCGAAAATCGACAAGGAGGCCAAGCTGGATCACTCTAAGTATTTGGTTCATCAGCTGCTTCCGTATTTGAAGATGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTGGAAGCCAAGAGGCAGGGCTTACGTGACCTTTCGGAGCTCAAGGTGGACAAGGCTAAAAGCGGTGGTGACGGAGTGCGTATGTACTGCAACAGCTGCAGCACTTCAATTTTTGATTTTCATAGGACTTGTCCCGGGTGTGAGTATGATCTGTGCCTCGGCTGCTGCCGGGAGATTCGAGATGGCAAGTGGAACAAGAGAGCTCAAGCGTCCACATTTGAGTGGAAATGGAGAGCAAGTGAAGGCGGAAACATTTTCTGCCCACCAACACACATGAGAGGTTGTGGTAAGCATAATTTAGAATTGAGATGCATTTTTCCTCAGAATCATGTCATGGAGTTGGTAGAGAAAGCAGAAGAAATAGATTCAAGTTACAATTTTTTGCCTGATTGTAGTGAAACATGTGCATATAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTCAAATAGTAGCAGCAGCTGTAAGTTAAGGAAAGCAGCTTCTAGAGTTGCTTCTGATAGTGACTACTTGTTCTGTCCAAGGGTTGGACAAATTCAAGATAAGGATTTTGGGCATTTTCAGTGGCATTGGAAGAGGGGAGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCTGTCTTGTGGCGGGCATTTAGACAGATAGAGAAAGTCAAGAATGATAGTGAGTTGACATTCCACGCCATTGATTGTTGGGATTGGTGTTCTATAGAAATCAACATCCTCCATTTCTTTAGTGGATATTCCAACGGACAGTCTCATAAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATCATCTGCTGAATTTTACAAACGTCTTCCCCGGCATTGTAAGGAATTTTTATGTTGTCTGCCTCTCAAGGAGTATACTCACCCCAACAAGGGAACTTTGAACCTTGCTACCTACTTGCCACCTACCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACAGGGAGATTCCGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACAACTGAACAACTTGCAACTATAGAGAAGTTGAAGAAAAAGCACAGGGAGCAAAACGAAAGAGAGATATTTGGGGATTGTCACACAAGTGCGGGAAATGGAAGTGGGCCTCAGCAAGAGTCAGCTATAGAGAAGTTGAAGAGAAAGTATAGGGAGCAAAACGAAAGAGAGATATTTGGGAATTGTCACACAAGTGCTGGAAGTGGAAGTGGGCCTCAGGATGAGTCAGCTGAAGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGATCCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTATGCATGTATATGGTAATCCTTTGCAGCATGTTACTCATGCTATACATGATCAGACTGTATATCTCACTGTGGAACACAAGAGGAAGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTTTCCAAAAACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTTGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACAAACCACAGAGCCCATGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCAGTTTGTGAAGCTATGAAGGAGATTTCTGGTGAGATTGTGACAAAGAGGGTAAGGGCTGTGAAGGCAAAGAGGGCAAAGGACGAGTCGATACACTGCCATCAGTGTCATAGATATGTTATGGGAAGAGTAGTGCAGTGCACAAGGTGTCTGGAAAGAGGTATGGGAATGAGGACGAGGTATTGTATCCCTTGTATACAGAGATGGTATCCGAACACATCAGAGGAAGCCATTGCTAAGGCTTGTCCTGTATGCCACGGCAACTGCAACTGCACAGCCTGCCTTCGTTTGGACGTGCCTGTCCGTTGTTTGCAGAATCTAGACCCCAAAATCGACAGGGGTACCAAGCTGGAGCACTGTAAGTATTTGGTTCATCAGCTGCTTCCCTATTTGAAGAGGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTCGAGGCTAAGAGGCGGGGATTACGTGATGTTTTGGAGCTCAAGGTGGAGAAGGCTAAGTGCGGTGAAGATGAGCGTATGTACTGCAACAACTGCAGCACTTCGATTTTTGATTTTCATAGGAGTTGTCCCAAGTGTGGGTTTGATCTGTGCCTCAGTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAAGAAGAGAGAGGTGGAGGAGGAAGCTCAAGTGTCCACATTTGAGTGGAGATTTAGTGAAGATGGAAGCATTCTCTGCCCCCCAACACAAAAGAAATGTTTTGGTAAGCATAATTTAGAATTGAGATGCATTTTTTCTCAGAATCATGTCATGGAATTGGTAGAGAAAGCAGGAAAAATAGATGCGAGTTACAATTTTTTGCCTGACCGTAGTGAAACATGTGCATACAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTCAAGCAGCAGCAGCTGTAAGTTAAGGAAAGCAGCTTCTAGAGTATCTTCTGACAGTAACTACTTGTTCTGTCCAAGGGTTGGGCAAATCCAAGATAAGGATTTTGGGCATTTTCAGTGGCATTGGATGAGGGGCGAGCCTGTGGTCGTTAGCAATGCATTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCCGTCATGTGGCGGGCACTTAGAAAGACAGTTAATAACTGCAATGCTAGTGAATTGACATTCCATGCCGTTGATTGCTGGGATTGGTGTTTTATAAGAATCAGAATCCGCCATTTCTTTAGTGGATATTCCAAGGGACGGTCTTATAAGACCGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATTATCTACTGAGTTTCACAATCGTCTTCCCCGGCATGGTACGGAATTATTATGTTGTCTGCCTTTCAAGGAGTATAGTCACCCCAACAAGGGAACTCTGAACCTTGCTACCTACTTGCCTCCTACCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACAGGGAGATTCTGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACAACTGAACAACTTGCAACTATAGAGAAGTTGAAGAAAAAGCACGGGAGCAAGACAAAAGAGAGATATTTGGGAATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCTCTGCAAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1387

Amino Acids

158.57

Weight (kDa)

8.75

Isoelectric Point (pI)

49.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 144 - 203 4.4e-07 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 703 - 797 2.6e-12 JmjC domain, hydroxylase
zf-4CXXC_R1 PF10497 863 - 931 3.5e-06 Zinc-finger domain of monoamine-oxidase A repressor R1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3677, 3729
AarI CACCTGC 2 cut(s) 718, 2908
AasI GACNNNNNNGTC 1 cut(s) 2804
Acc36I ACCTGC 5 cut(s) 339, 408, 718, 2324, 2908
Acc65I GGTACC 1 cut(s) 2846
AccB1I GGYRCC 2 cut(s) 2099, 2846
AccB7I CCANNNNNTGG 2 cut(s) 1277, 3470
AccBSI CCGCTC 1 cut(s) 473
AccI GTMKAC 2 cut(s) 1652, 2701
AccII CGCG 1 cut(s) 582
AccIII TCCGGA 1 cut(s) 2118
AclWI GGATC 6 cut(s) 118, 675, 1375, 2116, 2129, 3568
AcoI YGGCCR 1 cut(s) 50
AcsI RAATTY 6 cut(s) 66, 1284, 1589, 1622, 2171, 2273
AcuI CTGAAG 3 cut(s) 1857, 2100, 4050
AcyI GRCGYC 1 cut(s) 17
AdeI CACNNNGTG 2 cut(s) 489, 2652
AfaI GTAC 6 cut(s) 315, 426, 830, 2848, 3017, 3811
AflIII ACRYGT 2 cut(s) 1145, 3341
AjiI CACGTC 2 cut(s) 2164, 2800
AjnI CCWGG 3 cut(s) 9, 143, 2124
AleI CACNNNNGTG 3 cut(s) 1935, 2040, 4127
Alw21I GWGCWC 6 cut(s) 485, 786, 949, 2648, 2862, 2976
Alw26I GTCTC 1 cut(s) 1536
Alw44I GTGCAC 2 cut(s) 481, 2644
AlwI GGATC 6 cut(s) 118, 675, 1375, 2116, 2129, 3568
AlwNI CAGNNNCTG 3 cut(s) 1212, 3096, 3405
Ama87I CYCGRG 2 cut(s) 36, 878
Aor13HI TCCGGA 1 cut(s) 2118
ApaLI GTGCAC 2 cut(s) 481, 2644
ApoI RAATTY 6 cut(s) 66, 1284, 1589, 1622, 2171, 2273
ArsI GACNNNNNNTTYG 2 cut(s) 2576, 2608
Asp700I GAANNNNTTC 1 cut(s) 2436
Asp718I GGTACC 1 cut(s) 2846
AspS9I GGNCC 8 cut(s) 33, 1724, 1843, 1955, 2060, 3917, 4036, 4147
AsuC2I CCSGG 6 cut(s) 879, 880, 914, 1609, 3101, 3802
AvaI CYCGRG 2 cut(s) 36, 878
AvaII GGWCC 2 cut(s) 1724, 3917
AxyI CCTNAGG 1 cut(s) 2064
BaeGI GKGCMC 3 cut(s) 485, 2648, 3601
BaeI ACNNNNGTAYC 2 cut(s) 2830, 2863
BamHI GGATCC 1 cut(s) 2121
BanI GGYRCC 2 cut(s) 2099, 2846
BanII GRGCYC 6 cut(s) 277, 346, 786, 949, 2467, 2976
BarI GAAGNNNNNNTAC 4 cut(s) 1773, 1805, 3966, 3998
BbsI GAAGAC 2 cut(s) 1595, 3788
Bbv12I GWGCWC 6 cut(s) 485, 786, 949, 2648, 2862, 2976
BbvCI CCTCAGC 2 cut(s) 561, 1959
BceAI ACGGC 6 cut(s) 4, 1172, 2272, 2779, 3368, 3635
BciT130I CCWGG 3 cut(s) 11, 145, 2126
BciVI GTATCC 4 cut(s) 521, 543, 2702, 2724
BclI TGATCA 1 cut(s) 2224
BcnI CCSGG 6 cut(s) 879, 880, 914, 1609, 3101, 3802
BcoDI GTCTC 1 cut(s) 1536
BfaI CTAG 7 cut(s) 167, 324, 393, 1236, 2825, 3429, 3630
BfmI CTRYAG 9 cut(s) 332, 401, 841, 1482, 1869, 1974, 2331, 3028, 4062
BfuAI ACCTGC 5 cut(s) 339, 408, 718, 2324, 2908
BfuI GTATCC 4 cut(s) 521, 543, 2702, 2724
Bme1390I CCNGG 9 cut(s) 11, 145, 879, 880, 914, 1609, 2126, 3101, 3802
Bme18I GGWCC 2 cut(s) 1724, 3917
BmeT110I CYCGRG 2 cut(s) 36, 878
BmgBI CACGTC 2 cut(s) 2164, 2800
BmgT120I GGNCC 8 cut(s) 33, 1724, 1843, 1955, 2060, 3917, 4036, 4147
BmrFI CCNGG 9 cut(s) 11, 145, 879, 880, 914, 1609, 2126, 3101, 3802
BmsI GCATC 9 cut(s) 161, 1043, 1174, 1798, 2305, 3239, 3295, 3370, 3991
BoxI GACNNNNGTC 2 cut(s) 1722, 3915
BpiI GAAGAC 2 cut(s) 1595, 3788
BplI GAGNNNNNCTC 6 cut(s) 259, 291, 328, 360, 397, 429
BpmI CTGGAG 3 cut(s) 2162, 2501, 2876
Bpu10I CCTNAGC 3 cut(s) 561, 1959, 2742
BpuEI CTTGAG 9 cut(s) 770, 933, 1544, 1628, 2249, 2960, 3135, 3376, 3737
BpuMI CCSGG 6 cut(s) 879, 880, 914, 1609, 3101, 3802
BsaAI YACGTR 2 cut(s) 770, 2960
BsaHI GRCGYC 1 cut(s) 17
BsaWI WCCGGW 2 cut(s) 2118, 3734
Bse21I CCTNAGG 1 cut(s) 2064
Bse3DI GCAATG 5 cut(s) 604, 1357, 2736, 3550, 3631
BseAI TCCGGA 1 cut(s) 2118
BseBI CCWGG 3 cut(s) 11, 145, 2126
BseMI GCAATG 5 cut(s) 604, 1357, 2736, 3550, 3631
BseRI GAGGAG 1 cut(s) 3155
BseSI GKGCMC 3 cut(s) 485, 2648, 3601
BseX3I CGGCCG 1 cut(s) 50
BseYI CCCAGC 3 cut(s) 1380, 3573, 3660
BsgI GTGCAG 4 cut(s) 308, 377, 2660, 2761
Bsh1236I CGCG 1 cut(s) 582
Bsh1285I CGRYCG 1 cut(s) 53
BshNI GGYRCC 2 cut(s) 2099, 2846
BsiEI CGRYCG 1 cut(s) 53
BsiHKAI GWGCWC 6 cut(s) 485, 786, 949, 2648, 2862, 2976
BsiHKCI CYCGRG 2 cut(s) 36, 878
BsiSI CCGG 9 cut(s) 879, 913, 1609, 1715, 2119, 3100, 3735, 3802, 3908
BslFI GGGAC 4 cut(s) 861, 2123, 3048, 3732
BsmAI GTCTC 1 cut(s) 1536
BsmFI GGGAC 4 cut(s) 861, 2123, 3048, 3732
BsmI GAATGC 1 cut(s) 3192
BsoBI CYCGRG 2 cut(s) 36, 878
Bsp13I TCCGGA 1 cut(s) 2118
Bsp143I GATC 9 cut(s) 110, 667, 892, 1341, 1367, 2121, 2224, 3079, 3560
BspEI TCCGGA 1 cut(s) 2118
BspFNI CGCG 1 cut(s) 582
BspMAI CTGCAG 5 cut(s) 336, 405, 845, 2335, 3032
BspMI ACCTGC 5 cut(s) 339, 408, 718, 2324, 2908
BspPI GGATC 6 cut(s) 118, 675, 1375, 2116, 2129, 3568
BspQI GCTCTTC 1 cut(s) 463
BspT107I GGYRCC 2 cut(s) 2099, 2846
BsrBI CCGCTC 1 cut(s) 473
BsrDI GCAATG 5 cut(s) 604, 1357, 2736, 3550, 3631
BssMI GATC 9 cut(s) 110, 667, 892, 1341, 1367, 2121, 2224, 3079, 3560
BssNAI GTATAC 2 cut(s) 1653, 2702
BssNI GRCGYC 1 cut(s) 17
BssT1I CCWWGG 5 cut(s) 279, 348, 1271, 3464, 3714
Bst1107I GTATAC 2 cut(s) 1653, 2702
Bst2UI CCWGG 3 cut(s) 11, 145, 2126
Bst4CI ACNGT 8 cut(s) 1530, 2233, 2245, 2864, 3332, 3446, 3613, 3723
Bst6I CTCTTC 8 cut(s) 463, 494, 1319, 1982, 2427, 2626, 2894, 3122
BstACI GRCGYC 1 cut(s) 17
BstAPI GCANNNNNTGC 5 cut(s) 603, 840, 2784, 3027, 3198
BstBAI YACGTR 2 cut(s) 770, 2960
BstC8I GCNNGC 6 cut(s) 577, 1404, 2505, 2785, 3527, 3597
BstDSI CCRYGG 3 cut(s) 580, 2288, 2761
BstENI CCTNNNNNAGG 1 cut(s) 148
BstFNI CGCG 1 cut(s) 582
BstKTI GATC 9 cut(s) 113, 670, 895, 1344, 1370, 2124, 2227, 3082, 3563
BstMAI GTCTC 1 cut(s) 1536
BstMBI GATC 9 cut(s) 110, 667, 892, 1341, 1367, 2121, 2224, 3079, 3560
BstMCI CGRYCG 1 cut(s) 53
BstNI CCWGG 3 cut(s) 11, 145, 2126
BstNSI RCATGY 5 cut(s) 1149, 2183, 2207, 2507, 3345
BstPAI GACNNNNGTC 2 cut(s) 1722, 3915
BstSCI CCNGG 9 cut(s) 9, 143, 877, 878, 912, 1607, 2124, 3099, 3800
BstSFI CTRYAG 9 cut(s) 332, 401, 841, 1482, 1869, 1974, 2331, 3028, 4062
BstSLI GKGCMC 3 cut(s) 485, 2648, 3601
BstUI CGCG 1 cut(s) 582
BstV2I GAAGAC 2 cut(s) 1595, 3788
BstX2I RGATCY 1 cut(s) 2121
BstXI CCANNNNNNTGG 1 cut(s) 2309
BstYI RGATCY 1 cut(s) 2121
BstZ17I GTATAC 2 cut(s) 1653, 2702
BstZI CGGCCG 1 cut(s) 50
Bsu36I CCTNAGG 1 cut(s) 2064
BsuI GTATCC 4 cut(s) 521, 543, 2702, 2724
BtgI CCRYGG 3 cut(s) 580, 2288, 2761
BtrI CACGTC 2 cut(s) 2164, 2800
BtsI GCAGTG 5 cut(s) 125, 1792, 2599, 2648, 3985
BveI ACCTGC 5 cut(s) 339, 408, 718, 2324, 2908
Cac8I GCNNGC 6 cut(s) 577, 1404, 2505, 2785, 3527, 3597
CaiI CAGNNNCTG 3 cut(s) 1212, 3096, 3405
Cfr13I GGNCC 8 cut(s) 33, 1724, 1843, 1955, 2060, 3917, 4036, 4147
Cfr42I CCGCGG 1 cut(s) 583
Cfr9I CCCGGG 1 cut(s) 878
CseI GACGC 2 cut(s) 25, 942
Csp6I GTAC 6 cut(s) 314, 425, 829, 2847, 3016, 3810
CviQI GTAC 6 cut(s) 314, 425, 829, 2847, 3016, 3810
DpnI GATC 9 cut(s) 112, 669, 894, 1343, 1369, 2123, 2226, 3081, 3562
DpnII GATC 9 cut(s) 110, 667, 892, 1341, 1367, 2121, 2224, 3079, 3560
DraIII CACNNNGTG 2 cut(s) 489, 2652
DrdI GACNNNNNNGTC 1 cut(s) 2804
DseDI GACNNNNNNGTC 1 cut(s) 2804
EaeI YGGCCR 1 cut(s) 50
EagI CGGCCG 1 cut(s) 50
Eam1104I CTCTTC 8 cut(s) 463, 494, 1319, 1982, 2427, 2626, 2894, 3122
EarI CTCTTC 8 cut(s) 463, 494, 1319, 1982, 2427, 2626, 2894, 3122
EciI GGCGGA 3 cut(s) 175, 1005, 3680
Ecl136II GAGCTC 3 cut(s) 784, 947, 2974
EclXI CGGCCG 1 cut(s) 50
Eco130I CCWWGG 5 cut(s) 279, 348, 1271, 3464, 3714
Eco24I GRGCYC 6 cut(s) 277, 346, 786, 949, 2467, 2976
Eco47I GGWCC 2 cut(s) 1724, 3917
Eco52I CGGCCG 1 cut(s) 50
Eco53kI GAGCTC 3 cut(s) 784, 947, 2974
Eco57I CTGAAG 3 cut(s) 1857, 2100, 4050
Eco81I CCTNAGG 1 cut(s) 2064
Eco88I CYCGRG 2 cut(s) 36, 878
EcoICRI GAGCTC 3 cut(s) 784, 947, 2974
EcoNI CCTNNNNNAGG 1 cut(s) 148
EcoRI GAATTC 2 cut(s) 66, 2273
EcoRII CCWGG 3 cut(s) 9, 143, 2124
EcoT14I CCWWGG 5 cut(s) 279, 348, 1271, 3464, 3714
EcoT22I ATGCAT 4 cut(s) 1058, 2181, 3254, 3550
EcoT38I GRGCYC 6 cut(s) 277, 346, 786, 949, 2467, 2976
ErhI CCWWGG 5 cut(s) 279, 348, 1271, 3464, 3714
FalI AAGNNNNNCTT 6 cut(s) 1830, 1862, 2367, 2399, 4023, 4055
FaqI GGGAC 4 cut(s) 861, 2123, 3048, 3732
FauI CCCGC 4 cut(s) 1395, 1933, 2943, 3588
FauNDI CATATG 1 cut(s) 59
FbaI TGATCA 1 cut(s) 2224
FblI GTMKAC 2 cut(s) 1652, 2701
FriOI GRGCYC 6 cut(s) 277, 346, 786, 949, 2467, 2976
FspBI CTAG 7 cut(s) 167, 324, 393, 1236, 2825, 3429, 3630
GsaI CCCAGC 3 cut(s) 1384, 3577, 3664
GsuI CTGGAG 3 cut(s) 2162, 2501, 2876
HapII CCGG 9 cut(s) 879, 913, 1609, 1715, 2119, 3100, 3735, 3802, 3908
HgaI GACGC 2 cut(s) 25, 942
Hin1I GRCGYC 1 cut(s) 17
HincII GTYRAC 1 cut(s) 1446
HindII GTYRAC 1 cut(s) 1446
HindIII AAGCTT 3 cut(s) 134, 1787, 3980
HpaII CCGG 9 cut(s) 879, 913, 1609, 1715, 2119, 3100, 3735, 3802, 3908
Hpy166II GTNNAC 9 cut(s) 483, 793, 957, 1446, 1653, 2292, 2646, 2702, 3159
Hpy8I GTNNAC 9 cut(s) 483, 793, 957, 1446, 1653, 2292, 2646, 2702, 3159
Hpy99I CGWCG 4 cut(s) 19, 25, 80, 83
HpyCH4III ACNGT 8 cut(s) 1530, 2233, 2245, 2864, 3332, 3446, 3613, 3723
HpyCH4IV ACGT 5 cut(s) 769, 1600, 2163, 2799, 2959
HpySE526I ACGT 5 cut(s) 769, 1600, 2163, 2799, 2959
Hsp92I GRCGYC 1 cut(s) 17
Kpn2I TCCGGA 1 cut(s) 2118
KpnI GGTACC 1 cut(s) 2850
Ksp22I TGATCA 1 cut(s) 2224
KspI CCGCGG 1 cut(s) 583
Kzo9I GATC 9 cut(s) 110, 667, 892, 1341, 1367, 2121, 2224, 3079, 3560
LguI GCTCTTC 1 cut(s) 463
LweI GCATC 9 cut(s) 161, 1043, 1174, 1798, 2305, 3239, 3295, 3370, 3991
MaeI CTAG 7 cut(s) 167, 324, 393, 1236, 2825, 3429, 3630
MaeII ACGT 5 cut(s) 769, 1600, 2163, 2799, 2959
MalI GATC 9 cut(s) 112, 669, 894, 1343, 1369, 2123, 2226, 3081, 3562
MbiI CCGCTC 1 cut(s) 473
MboI GATC 9 cut(s) 110, 667, 892, 1341, 1367, 2121, 2224, 3079, 3560
MfeI CAATTG 1 cut(s) 436
MflI RGATCY 1 cut(s) 2121
MlyI GAGTC 4 cut(s) 406, 1976, 2081, 2600
MmeI TCCRAC 3 cut(s) 1258, 1545, 2474
Mph1103I ATGCAT 4 cut(s) 1058, 2181, 3254, 3550
MroI TCCGGA 1 cut(s) 2118
MroXI GAANNNNTTC 1 cut(s) 2436
MseI TTAA 9 cut(s) 1170, 1220, 1376, 1815, 3366, 3413, 3569, 3615, 4008
MspI CCGG 9 cut(s) 879, 913, 1609, 1715, 2119, 3100, 3735, 3802, 3908
MspR9I CCNGG 9 cut(s) 11, 145, 879, 880, 914, 1609, 2126, 3101, 3802
MunI CAATTG 1 cut(s) 436
Mva1269I GAATGC 1 cut(s) 3192
MvaI CCWGG 3 cut(s) 11, 145, 2126
MvnI CGCG 1 cut(s) 582
NciI CCSGG 6 cut(s) 879, 880, 914, 1609, 3101, 3802
NdeI CATATG 1 cut(s) 59
NdeII GATC 9 cut(s) 110, 667, 892, 1341, 1367, 2121, 2224, 3079, 3560
NmeAIII GCCGAG 1 cut(s) 882
NmuCI GTSAC 8 cut(s) 770, 812, 1253, 1928, 2033, 2545, 3848, 4120
NsiI ATGCAT 4 cut(s) 1058, 2181, 3254, 3550
NspI RCATGY 5 cut(s) 1149, 2183, 2207, 2507, 3345
OliI CACNNNNGTG 3 cut(s) 1935, 2040, 4127
PaeI GCATGC 1 cut(s) 2507
PaqCI CACCTGC 2 cut(s) 718, 2908
PasI CCCWGGG 1 cut(s) 144
PciI ACATGT 2 cut(s) 1145, 3341
PciSI GCTCTTC 1 cut(s) 463
PctI GAATGC 1 cut(s) 3192
PdmI GAANNNNTTC 1 cut(s) 2436
PflFI GACNNNGTC 1 cut(s) 19
PflMI CCANNNNNTGG 2 cut(s) 1277, 3470
PfoI TCCNGGA 1 cut(s) 2124
PleI GAGTC 4 cut(s) 406, 1975, 2080, 2599
PpsI GAGTC 4 cut(s) 406, 1975, 2080, 2599
Ppu21I YACGTR 2 cut(s) 770, 2960
PscI ACATGT 2 cut(s) 1145, 3341
PshAI GACNNNNGTC 2 cut(s) 1722, 3915
PsiI TTATAA 2 cut(s) 3677, 3729
Psp124BI GAGCTC 3 cut(s) 786, 949, 2976
Psp6I CCWGG 3 cut(s) 9, 143, 2124
PspFI CCCAGC 3 cut(s) 1380, 3573, 3660
PspGI CCWGG 3 cut(s) 9, 143, 2124
PspPI GGNCC 8 cut(s) 33, 1724, 1843, 1955, 2060, 3917, 4036, 4147
PstI CTGCAG 5 cut(s) 336, 405, 845, 2335, 3032
PstNI CAGNNNCTG 3 cut(s) 1212, 3096, 3405
PsuI RGATCY 1 cut(s) 2121
PsyI GACNNNGTC 1 cut(s) 19
PvuII CAGCTG 9 cut(s) 694, 840, 1212, 1835, 2078, 2140, 2884, 3405, 4028
RsaI GTAC 6 cut(s) 315, 426, 830, 2848, 3017, 3811
RsaNI GTAC 6 cut(s) 314, 425, 829, 2847, 3016, 3810
SacI GAGCTC 3 cut(s) 786, 949, 2976
SacII CCGCGG 1 cut(s) 583
SapI GCTCTTC 1 cut(s) 463
SaqAI TTAA 9 cut(s) 1170, 1220, 1376, 1815, 3366, 3413, 3569, 3615, 4008
Sau3AI GATC 9 cut(s) 110, 667, 892, 1341, 1367, 2121, 2224, 3079, 3560
Sau96I GGNCC 8 cut(s) 33, 1724, 1843, 1955, 2060, 3917, 4036, 4147
SbfI CCTGCAGG 1 cut(s) 2335
SchI GAGTC 4 cut(s) 406, 1976, 2081, 2600
ScrFI CCNGG 9 cut(s) 11, 145, 879, 880, 914, 1609, 2126, 3101, 3802
SdaI CCTGCAGG 1 cut(s) 2335
SfaNI GCATC 9 cut(s) 161, 1043, 1174, 1798, 2305, 3239, 3295, 3370, 3991
SfcI CTRYAG 9 cut(s) 332, 401, 841, 1482, 1869, 1974, 2331, 3028, 4062
Sfr303I CCGCGG 1 cut(s) 583
SgrBI CCGCGG 1 cut(s) 583
SinI GGWCC 2 cut(s) 1724, 3917
SmaI CCCGGG 1 cut(s) 880
SmlI CTYRAG 9 cut(s) 785, 948, 1559, 1643, 2264, 2975, 3150, 3391, 3752
SmoI CTYRAG 9 cut(s) 785, 948, 1559, 1643, 2264, 2975, 3150, 3391, 3752
SphI GCATGC 1 cut(s) 2507
Sse8387I CCTGCAGG 1 cut(s) 2335
SspI AATATT 2 cut(s) 1819, 4012
SspMI CTAG 7 cut(s) 167, 324, 393, 1236, 2825, 3429, 3630
SstI GAGCTC 3 cut(s) 786, 949, 2976
StyD4I CCNGG 9 cut(s) 9, 143, 877, 878, 912, 1607, 2124, 3099, 3800
StyI CCWWGG 5 cut(s) 279, 348, 1271, 3464, 3714
TaaI ACNGT 8 cut(s) 1530, 2233, 2245, 2864, 3332, 3446, 3613, 3723
TaiI ACGT 5 cut(s) 772, 1603, 2166, 2802, 2962
TatI WGTACW 2 cut(s) 828, 3015
TauI GCSGC 4 cut(s) 52, 160, 582, 585
Tru1I TTAA 9 cut(s) 1170, 1220, 1376, 1815, 3366, 3413, 3569, 3615, 4008
Tru9I TTAA 9 cut(s) 1170, 1220, 1376, 1815, 3366, 3413, 3569, 3615, 4008
TseFI GTSAC 8 cut(s) 770, 812, 1253, 1928, 2033, 2545, 3848, 4120
Tsp45I GTSAC 8 cut(s) 770, 812, 1253, 1928, 2033, 2545, 3848, 4120
TspMI CCCGGG 1 cut(s) 878
Tth111I GACNNNGTC 1 cut(s) 19
Van91I CCANNNNNTGG 2 cut(s) 1277, 3470
VneI GTGCAC 2 cut(s) 481, 2644
VpaK11BI GGWCC 2 cut(s) 1724, 3917
XagI CCTNNNNNAGG 1 cut(s) 148
XapI RAATTY 6 cut(s) 66, 1284, 1589, 1622, 2171, 2273
XbaI TCTAGA 3 cut(s) 1235, 2824, 3428
XceI RCATGY 5 cut(s) 1149, 2183, 2207, 2507, 3345
XcmI CCANNNNNNNNNTGG 4 cut(s) 1395, 1508, 3588, 3701
XmaI CCCGGG 1 cut(s) 878
XmiI GTMKAC 2 cut(s) 1652, 2701
XmnI GAANNNNTTC 1 cut(s) 2436
XspI CTAG 7 cut(s) 167, 324, 393, 1236, 2825, 3429, 3630
Zsp2I ATGCAT 4 cut(s) 1058, 2181, 3254, 3550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.