RLG00000001919

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000280
Physical Location & Seq
Reverse (-)
23011108 .. 23017506
6399 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001919

Sequence Viewer

Length: 4818 bp
ATGAAATTCAAGGCGACGGCGGCGAGATTGAGGCCCGGAGGCACAAAGCGGCGGAACGGCGGTGGTGTGGGTATTTTGGGAATTCAGAACGACGACGGAACAAATGGTGGAGGACCAAAGCTTGTTCCCTGGGGGAGGAAGCGGCGGAGGACAGGATGCGAGGGTATTTTGGGATATCAAAATGAAGGAGTGGCAAGGGCTAAGAAGGCGAAGAAGACGAAGCAGAAAAGGGCTTACTTGAAGAAGGAGTTGACAATATGCCATCAATGTAGGAAAACAGGTAAGGGAAGAACGGTGCGGTGCACATGGTGTTCGAGGAACAGGTATTGTATCCCTTGCATACAGAGGTGGTATCCCAACACATCAGAGGAAGCCATAGCTGAGGCTTGTCCTGTGTGCCGCGGCAACTGCAACTGCTATACCTGCCTTCGATTGGATTTGCCTAACAGGTGTTTGAAGAATCTAGACCCGAAAATCGATAAGGAGACCAAGCTGGAGCACTGTAGGTATATGGTTCATCAGCTGCTTCCGTATCTGAAGATGATTAGGGATGAGCAGGTGTGTGAGATGGTGGTCGAGGCTAATAGGCAGGGTTTAGGTGATGATGTTTCGGAGCTCAAGGTGGACAGGGCTAATTGCGGTGAAGACGAGCGTATGTACTGCAACAACTGCAGCACTTCGATTTTTGATTTTCATAGGACTTGTCCTGAGTGTGAGTATGATCTGTGCCTCGGTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAACAAGAGAGCGGAAATGTCCACATTTGAGTGGAGAACAAGTGAAGATGGAAGCATTCTCTGCCCCCCAACACACATGGAAGGTTGTGGTAAGCATAATTTAGAATTGAGATGCCTCTTTCCTCAGAATCATGTCATGGAATTGGTAGAGAATGCAGGAAAAATAGATGCAAGTTACAATTTTTTGCCTGATCGTAGTGAAACATGTGCATATAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTTGCTCAACTAGCAGCAGCTCTAAGTTAAGGAAAGCAGCTTCTAGAGTTGCTTCTGATGATAACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAGGATAAGGATTTTGACCATTTTCAGTGGCATTGGAAGAGGGGTGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCTGTCATGTGGCGGGCATTTAAGCAGACAAATAATATCAACCATGCTAGTCAATCGACATTCCATGCCATTGATTGCTGGTATTGGTGTTTTATAGAAATCCACATCCTCCATTTCTGTAGTGGATATTCCAAGGGACGGTCTTATAAGACAGGATGGCCTCAGAATCTCAAGCTCACTGACTTCCCATCATCTACTGAATTTCACAATCGTCTTCCCCGGCATGGTGAGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATACTCACCCCAACAAGGGAACTCTGAACCTTGCTATCTACTTGCCACCTATCTCAGTAAAGCCGGACATGGGTCCAAAAACGTATATTGCCTATGGTTTTGCAGAGGAGCTTGGACGGGGAGATTCCGTAACTAAGCTTCACTGCAATATGTCTGATGCGGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACATCTGAAGAACTTGCAACTATAGATAAGTTGAAGAAAAAGCTGAGGGAGCAAGACGAAAGAGAGATATTTGGAAATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCTCAGCAAGAGTCAGCTATAGAGAAGTTGAAGAGAAAGTGTAGGGAGCAAAACGAAAGAGAGATATTTGGGAATTGTCATACAAGTGCTGGAAATGGAAGTGGGCCTCAGCAAGAGTCAGCTGAAATATGTGAGGATGGAGGTGCTTTATGGGACATTTTCCGGATCCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTATGGATGTGCTCTGTAATCCTTTGCAGCATGTTACTCATGCTATACACGATCAGACTGTCTATCTCACTGTGGAGCACAAGAGGAAGCTGAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATGCTGTTTTCATTCCTGCTGGTTGTCCTCATCAGGTCAGGAACTTGAAGTCTTGTATTAAAGTTGCACTGGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCAACAAACCACAGAGCCCATGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTATGAAGGAGATTCCTTGTGAGAATGTGACAAAGAGGGTAAGGCCTGTGAAGGCAAAGAGGGCAAGGGTTCGCTTGGACGAGTTGAGACGTTGCCATCAGTGTCATATGAATGTTATGGGAAGAGTGGTGCAATGCACAAGGTGTCTGGAAAGAGGTACGGGAAGGAGGACAAGGTATTGTATCCCTTGTATACAGAGATGGTATCCCAACACATCAGAGGAAGCCATTGCTAAGGCTTGTCCTTTATGCTGTGGCAACTGCAGCTGCACAGCCTGTCTTCGCTTGGATGTGCCTGTCTGGTGTTTGCAGAATCTAGAGCCAAAAAGTGACAAGGGTACCAAGCTGGAGCACTCTAAGTATTTGGTTCATCTGCTGCTTCCCTATTTGAAGATGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTGGAAGCCAAGAGGCAGGGCTTACGTGACCTTTCGGAGCCCAAGGTGGAGAAGGCTAAAATCAGTGACGGAGTGTGTATGTACTGCAACAGCTGCAGCACTTCGATTTTTGATTTTCATAGGACTTGTCCCGGATGTGAGTATGATCTGTGCCTCGGTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAAGAAGAGAGCTCAAGCGTCCGCATTTGAGTGGAAATGGAGAGCAAGTGAAGATGGAAGCATTCTCTGCCCCCCAACACACATGAAAGGTTGTGGTAAGCACAATTTAGAATTGAGATGCATTTTTTCTCAGAATCATGTCATGGAATTGGTAGAGAAAGCAGAAGAAATAGATGCAAGTTACAATTTTTTGCCTGATCGTAGTGAAACATGTGCATATAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTTAAGTAGTAGCAGCTGCTGTAAGTTAAGGAAAGCAGCTTCTAGAGTTGCTTCTGATAGTAACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAAGATAAGGATTTTGAGCATTTTCAGTGGCATTGGAAGAGGGGTGAGCCTGTGATCGTTAGCAATGCGCTTGAAAATGGATCAGGTTTAAGCTGGGAACCATATGTCATGTGGCAGGCATTTAGAAAGACAAATAATATCAACCATGCTGGTGAATCGACAGTCCATGCCATTGATTGCTGGGATTGGTGTTTTATAGAAATCAACATCCACCATTTCTTTAGTGGATATTCCAATGGACGGTGTTATAAGACAGGATGGCCTCAGAATCTAAAGCTCAATGACTTCCCATCATCTACTGAGTTTCACAATCGTCTTCCCCGGCATGGTAGGGAATTTTTATGTTGTCTGCCTTTCAAAGAGTATACTCACCCCAACAAGGGAACTCTGAACCTTGCTATCAACTTGCCACCTATCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCAGAGGAGCTTGGACGGGGAGATTCTGTAACCAAGCTTCACTGCAATATGTCCGATGCGGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACAACTGAACAACTTGTAACTGTAGAGAAGTTGAAGAAAAAGCACAGGGAGCAAGACCAAAGAGAGATATTTGGGAATTGTCACTCAAGTGCTGGAAATGGAAGTGGGTCTCAGGAAGAATTAGCTGATGTATGTGAGGATGGAGGTGCCTTATGGGACATATTCCGGACTCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTAAGCATGTGTTCTGTAATCCTTTGCAGCATGTTACTCATGCTATACATGATCAGACTGTTTATCTCACTGTGGAGCACAAGAGGAAGCTGAAGGAGGAATTCAGTATCGAGCCTTGGACATTTGTCCAAAAACCTGGAGATGCTGTTTTCATTCCTGCTGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTGGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACATACCACAGAGCCCATGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTATTTGTGAAGCTGTGAAGGAGATTTCTGGTGAGAATGTGATGACTGAGGATGGGAAGTCTAGGGGAGGCGCAAGGGAATTAAGGCAAGGTAGTTCCACAAGGAGATGCTATATTCATAAAGGTTGTTTACAGCCTTTTGTCTTTGAAGTAGCTCTTGCAGTTTCTCATTTGGGAATGTTTCGTTGTTTTGTGCACTACTTGAAGTATATTTTGCTTGTAGTCTGGATTGAAGCCACGCACTTTGGTTTACTACTGAGCTGTCACATATCACAGGCCTTTCAACTATATAACATTTATGTCAAGAGGTGTGTGCCACCAATCAGATGTGCTCGTACAGTATCTATTCTGCAACCAAAAACTTGGCATGAGGAACAAGGATCAAACCTCCCAAATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1606

Amino Acids

183.28

Weight (kDa)

8.36

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 81 - 144 2.9e-06 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 650 - 737 5.8e-13 JmjC domain, hydroxylase
JmjC PF02373 1337 - 1434 1.2e-13 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1356, 3552
AarI CACCTGC 2 cut(s) 547, 2737
Acc36I ACCTGC 3 cut(s) 431, 547, 2737
Acc65I GGTACC 1 cut(s) 2675
AccB1I GGYRCC 2 cut(s) 2675, 4010
AccB7I CCANNNNNTGG 2 cut(s) 1097, 3293
AccBSI CCGCTC 1 cut(s) 776
AccI GTMKAC 3 cut(s) 1472, 2530, 3668
AccII CGCG 1 cut(s) 402
AccIII TCCGGA 2 cut(s) 1938, 4029
AclWI GGATC 5 cut(s) 1195, 1936, 1949, 3391, 4804
AcsI RAATTY 9 cut(s) 5, 81, 1409, 1442, 1991, 2093, 3638, 4082, 4184
AcuI CTGAAG 6 cut(s) 557, 1677, 1695, 2105, 3873, 4196
AdeI CACNNNGTG 2 cut(s) 309, 2481
AfaI GTAC 5 cut(s) 659, 2497, 2677, 2846, 4753
AflII CTTAAG 1 cut(s) 3211
AflIII ACRYGT 2 cut(s) 968, 3161
AjiI CACGTC 2 cut(s) 1984, 4075
AjnI CCWGG 4 cut(s) 128, 1107, 1944, 4219
AleI CACNNNNGTG 2 cut(s) 1755, 3951
Alw26I GTCTC 3 cut(s) 479, 2419, 3978
Alw44I GTGCAC 2 cut(s) 301, 4610
AlwI GGATC 5 cut(s) 1195, 1936, 1949, 3391, 4804
AlwNI CAGNNNCTG 1 cut(s) 3228
Aor13HI TCCGGA 2 cut(s) 1938, 4029
AoxI GGCC 9 cut(s) 32, 1367, 1662, 1775, 1880, 2381, 3563, 3858, 4692
ApaLI GTGCAC 2 cut(s) 301, 4610
ApoI RAATTY 9 cut(s) 5, 81, 1409, 1442, 1991, 2093, 3638, 4082, 4184
Asp700I GAANNNNTTC 2 cut(s) 2256, 4347
Asp718I GGTACC 1 cut(s) 2675
AspLEI GCGC 2 cut(s) 3373, 4490
AspS9I GGNCC 8 cut(s) 33, 113, 1544, 1663, 1775, 1880, 3740, 3859
AsuC2I CCSGG 6 cut(s) 36, 743, 1429, 2895, 2930, 3625
AvaII GGWCC 3 cut(s) 113, 1544, 3740
BaeGI GKGCMC 2 cut(s) 305, 4614
BaeI ACNNNNGTAYC 2 cut(s) 2659, 2692
BamHI GGATCC 1 cut(s) 1941
BanI GGYRCC 2 cut(s) 2675, 4010
BanII GRGCYC 5 cut(s) 618, 2287, 2805, 2965, 4378
BarI GAAGNNNNNNTAC 4 cut(s) 1593, 1625, 3789, 3821
BbsI GAAGAC 5 cut(s) 221, 651, 1415, 2609, 3611
BbvCI CCTCAGC 4 cut(s) 381, 1712, 1779, 1884
BceAI ACGGC 5 cut(s) 33, 73, 995, 2092, 3188
BciT130I CCWGG 4 cut(s) 130, 1109, 1946, 4221
BciVI GTATCC 4 cut(s) 341, 363, 2531, 2553
BclI TGATCA 1 cut(s) 4135
BcnI CCSGG 6 cut(s) 36, 743, 1429, 2895, 2930, 3625
BcoDI GTCTC 3 cut(s) 479, 2419, 3978
BfaI CTAG 7 cut(s) 464, 1023, 1056, 1257, 2654, 3252, 4479
BfmI CTRYAG 8 cut(s) 502, 670, 1327, 1689, 1794, 2599, 2857, 3885
BfrI CTTAAG 1 cut(s) 3211
BfuAI ACCTGC 3 cut(s) 431, 547, 2737
BfuI GTATCC 4 cut(s) 341, 363, 2531, 2553
Bme18I GGWCC 3 cut(s) 113, 1544, 3740
BmgBI CACGTC 2 cut(s) 1984, 4075
BmgT120I GGNCC 8 cut(s) 33, 113, 1544, 1663, 1775, 1880, 3740, 3859
BmiI GGNNCC 8 cut(s) 1206, 1545, 1943, 2677, 2802, 3402, 3741, 4012
BoxI GACNNNNGTC 4 cut(s) 1542, 2117, 3738, 4208
BpiI GAAGAC 5 cut(s) 221, 651, 1415, 2609, 3611
BpmI CTGGAG 7 cut(s) 515, 1982, 2321, 2705, 4073, 4242, 4412
Bpu10I CCTNAGC 5 cut(s) 381, 1712, 1779, 1884, 2571
BpuEI CTTGAG 4 cut(s) 602, 1364, 2949, 3934
BpuMI CCSGG 6 cut(s) 36, 743, 1429, 2895, 2930, 3625
Bsa29I ATCGAT 1 cut(s) 477
BsaAI YACGTR 1 cut(s) 2789
BsaI GGTCTC 2 cut(s) 479, 3978
BsaWI WCCGGW 2 cut(s) 1938, 4029
Bse1I ACTGG 2 cut(s) 2208, 4299
Bse3DI GCAATG 4 cut(s) 1177, 2477, 2565, 3373
BseAI TCCGGA 2 cut(s) 1938, 4029
BseBI CCWGG 4 cut(s) 130, 1109, 1946, 4221
BseCI ATCGAT 1 cut(s) 477
BseMI GCAATG 4 cut(s) 1177, 2477, 2565, 3373
BseNI ACTGG 2 cut(s) 2208, 4299
BseRI GAGGAG 2 cut(s) 1592, 3788
BseSI GKGCMC 2 cut(s) 305, 4614
BseYI CCCAGC 3 cut(s) 1200, 3396, 3483
BsgI GTGCAG 1 cut(s) 2590
Bsh1236I CGCG 1 cut(s) 402
BshFI GGCC 9 cut(s) 34, 1369, 1664, 1777, 1882, 2383, 3565, 3860, 4694
BshNI GGYRCC 2 cut(s) 2675, 4010
BshVI ATCGAT 1 cut(s) 477
BslFI GGGAC 4 cut(s) 1359, 1943, 2877, 4034
BsmAI GTCTC 3 cut(s) 479, 2419, 3978
BsmBI CGTCTC 1 cut(s) 2419
BsmFI GGGAC 4 cut(s) 1359, 1943, 2877, 4034
BsmI GAATGC 3 cut(s) 819, 922, 3012
BsnI GGCC 9 cut(s) 34, 1369, 1664, 1777, 1882, 2383, 3565, 3860, 4694
Bso31I GGTCTC 2 cut(s) 479, 3978
Bsp13I TCCGGA 2 cut(s) 1938, 4029
BspANI GGCC 9 cut(s) 34, 1369, 1664, 1777, 1882, 2383, 3565, 3860, 4694
BspDI ATCGAT 1 cut(s) 477
BspEI TCCGGA 2 cut(s) 1938, 4029
BspFNI CGCG 1 cut(s) 402
BspLI GGNNCC 8 cut(s) 1206, 1545, 1943, 2677, 2802, 3402, 3741, 4012
BspMAI CTGCAG 3 cut(s) 674, 2603, 2861
BspMI ACCTGC 3 cut(s) 431, 547, 2737
BspPI GGATC 5 cut(s) 1195, 1936, 1949, 3391, 4804
BspT107I GGYRCC 2 cut(s) 2675, 4010
BspTI CTTAAG 1 cut(s) 3211
BspTNI GGTCTC 2 cut(s) 479, 3978
BsrBI CCGCTC 1 cut(s) 776
BsrDI GCAATG 4 cut(s) 1177, 2477, 2565, 3373
BsrI ACTGG 2 cut(s) 2208, 4299
BssNAI GTATAC 3 cut(s) 1473, 2531, 3669
BssT1I CCWWGG 5 cut(s) 1091, 1341, 2805, 3287, 4199
Bst1107I GTATAC 3 cut(s) 1473, 2531, 3669
Bst2UI CCWGG 4 cut(s) 130, 1109, 1946, 4221
Bst6I CTCTTC 7 cut(s) 1139, 1802, 2247, 2455, 2951, 3335, 4338
BstAFI CTTAAG 1 cut(s) 3211
BstAPI GCANNNNNTGC 4 cut(s) 669, 825, 2856, 3018
BstBAI YACGTR 1 cut(s) 2789
BstC8I GCNNGC 4 cut(s) 1224, 2325, 3420, 4416
BstDSI CCRYGG 2 cut(s) 400, 2108
BstENI CCTNNNNNAGG 1 cut(s) 133
BstFNI CGCG 1 cut(s) 402
BstHHI GCGC 2 cut(s) 3373, 4490
BstMAI GTCTC 3 cut(s) 479, 2419, 3978
BstNI CCWGG 4 cut(s) 130, 1109, 1946, 4221
BstNSI RCATGY 7 cut(s) 972, 2027, 2327, 3165, 4094, 4118, 4418
BstPAI GACNNNNGTC 4 cut(s) 1542, 2117, 3738, 4208
BstSFI CTRYAG 8 cut(s) 502, 670, 1327, 1689, 1794, 2599, 2857, 3885
BstSLI GKGCMC 2 cut(s) 305, 4614
BstUI CGCG 1 cut(s) 402
BstV2I GAAGAC 5 cut(s) 221, 651, 1415, 2609, 3611
BstX2I RGATCY 1 cut(s) 1941
BstXI CCANNNNNNTGG 3 cut(s) 2129, 4220, 4779
BstYI RGATCY 1 cut(s) 1941
BstZ17I GTATAC 3 cut(s) 1473, 2531, 3669
Bsu15I ATCGAT 1 cut(s) 477
BsuI GTATCC 4 cut(s) 341, 363, 2531, 2553
BsuRI GGCC 9 cut(s) 34, 1369, 1664, 1777, 1882, 2383, 3565, 3860, 4694
BsuTUI ATCGAT 1 cut(s) 477
BtgI CCRYGG 2 cut(s) 400, 2108
BtrI CACGTC 2 cut(s) 1984, 4075
BtsI GCAGTG 2 cut(s) 1612, 3808
BveI ACCTGC 3 cut(s) 431, 547, 2737
Cac8I GCNNGC 4 cut(s) 1224, 2325, 3420, 4416
CaiI CAGNNNCTG 1 cut(s) 3228
CfoI GCGC 2 cut(s) 3373, 4490
Cfr13I GGNCC 8 cut(s) 33, 113, 1544, 1663, 1775, 1880, 3740, 3859
Cfr42I CCGCGG 1 cut(s) 403
ClaI ATCGAT 1 cut(s) 477
CseI GACGC 1 cut(s) 2958
Csp6I GTAC 5 cut(s) 658, 2496, 2676, 2845, 4752
CspCI CAANNNNNGTGG 2 cut(s) 4642, 4677
CviQI GTAC 5 cut(s) 658, 2496, 2676, 2845, 4752
DraIII CACNNNGTG 2 cut(s) 309, 2481
Eam1104I CTCTTC 7 cut(s) 1139, 1802, 2247, 2455, 2951, 3335, 4338
EarI CTCTTC 7 cut(s) 1139, 1802, 2247, 2455, 2951, 3335, 4338
EciI GGCGGA 2 cut(s) 67, 160
Ecl136II GAGCTC 2 cut(s) 616, 2963
Eco130I CCWWGG 5 cut(s) 1091, 1341, 2805, 3287, 4199
Eco147I AGGCCT 2 cut(s) 2383, 4694
Eco24I GRGCYC 5 cut(s) 618, 2287, 2805, 2965, 4378
Eco31I GGTCTC 2 cut(s) 479, 3978
Eco32I GATATC 1 cut(s) 176
Eco47I GGWCC 3 cut(s) 113, 1544, 3740
Eco53kI GAGCTC 2 cut(s) 616, 2963
Eco57I CTGAAG 6 cut(s) 557, 1677, 1695, 2105, 3873, 4196
EcoICRI GAGCTC 2 cut(s) 616, 2963
EcoNI CCTNNNNNAGG 1 cut(s) 133
EcoRI GAATTC 3 cut(s) 81, 2093, 4184
EcoRII CCWGG 4 cut(s) 128, 1107, 1944, 4219
EcoRV GATATC 1 cut(s) 176
EcoT14I CCWWGG 5 cut(s) 1091, 1341, 2805, 3287, 4199
EcoT22I ATGCAT 1 cut(s) 3074
EcoT38I GRGCYC 5 cut(s) 618, 2287, 2805, 2965, 4378
ErhI CCWWGG 5 cut(s) 1091, 1341, 2805, 3287, 4199
Esp3I CGTCTC 1 cut(s) 2419
FalI AAGNNNNNCTT 6 cut(s) 1650, 1682, 3846, 3878, 4557, 4589
FaqI GGGAC 4 cut(s) 1359, 1943, 2877, 4034
FauI CCCGC 1 cut(s) 1215
FauNDI CATATG 2 cut(s) 2445, 3406
FbaI TGATCA 1 cut(s) 4135
FblI GTMKAC 3 cut(s) 1472, 2530, 3668
FriOI GRGCYC 5 cut(s) 618, 2287, 2805, 2965, 4378
FspBI CTAG 7 cut(s) 464, 1023, 1056, 1257, 2654, 3252, 4479
GlaI GCGC 2 cut(s) 3372, 4489
GsaI CCCAGC 3 cut(s) 1204, 3400, 3487
GsuI CTGGAG 7 cut(s) 515, 1982, 2321, 2705, 4073, 4242, 4412
HaeIII GGCC 9 cut(s) 34, 1369, 1664, 1777, 1882, 2383, 3565, 3860, 4694
HgaI GACGC 1 cut(s) 2958
HhaI GCGC 2 cut(s) 3373, 4490
Hin6I GCGC 2 cut(s) 3371, 4488
HinP1I GCGC 2 cut(s) 3371, 4488
HincII GTYRAC 1 cut(s) 252
HindII GTYRAC 1 cut(s) 252
HindIII AAGCTT 3 cut(s) 119, 1607, 3803
Hpy99I CGWCG 3 cut(s) 19, 95, 98
HpyCH4IV ACGT 5 cut(s) 1553, 1983, 2428, 2788, 4074
HpySE526I ACGT 5 cut(s) 1553, 1983, 2428, 2788, 4074
HspAI GCGC 2 cut(s) 3371, 4488
Kpn2I TCCGGA 2 cut(s) 1938, 4029
KpnI GGTACC 1 cut(s) 2679
Ksp22I TGATCA 1 cut(s) 4135
KspI CCGCGG 1 cut(s) 403
MaeI CTAG 7 cut(s) 464, 1023, 1056, 1257, 2654, 3252, 4479
MaeII ACGT 5 cut(s) 1553, 1983, 2428, 2788, 4074
MbiI CCGCTC 1 cut(s) 776
MflI RGATCY 1 cut(s) 1941
MlyI GAGTC 3 cut(s) 1796, 1901, 4027
MmeI TCCRAC 4 cut(s) 1078, 2294, 3274, 4385
Mph1103I ATGCAT 1 cut(s) 3074
MroI TCCGGA 2 cut(s) 1938, 4029
MroXI GAANNNNTTC 2 cut(s) 2256, 4347
MspCI CTTAAG 1 cut(s) 3211
Mva1269I GAATGC 3 cut(s) 819, 922, 3012
MvaI CCWGG 4 cut(s) 130, 1109, 1946, 4221
MvnI CGCG 1 cut(s) 402
NciI CCSGG 6 cut(s) 36, 743, 1429, 2895, 2930, 3625
NdeI CATATG 2 cut(s) 2445, 3406
NlaIV GGNNCC 8 cut(s) 1206, 1545, 1943, 2677, 2802, 3402, 3741, 4012
NmuCI GTSAC 7 cut(s) 1748, 2365, 2666, 2789, 2828, 3944, 4679
NsiI ATGCAT 1 cut(s) 3074
NspI RCATGY 7 cut(s) 972, 2027, 2327, 3165, 4094, 4118, 4418
OliI CACNNNNGTG 2 cut(s) 1755, 3951
PaeI GCATGC 2 cut(s) 2327, 4418
PaqCI CACCTGC 2 cut(s) 547, 2737
PasI CCCWGGG 1 cut(s) 129
PceI AGGCCT 2 cut(s) 2383, 4694
PciI ACATGT 2 cut(s) 968, 3161
PctI GAATGC 3 cut(s) 819, 922, 3012
PdmI GAANNNNTTC 2 cut(s) 2256, 4347
PflMI CCANNNNNTGG 2 cut(s) 1097, 3293
PfoI TCCNGGA 3 cut(s) 1107, 1944, 2893
PleI GAGTC 3 cut(s) 1795, 1900, 4027
PpsI GAGTC 3 cut(s) 1795, 1900, 4027
Ppu21I YACGTR 1 cut(s) 2789
PscI ACATGT 2 cut(s) 968, 3161
PshAI GACNNNNGTC 4 cut(s) 1542, 2117, 3738, 4208
PsiI TTATAA 2 cut(s) 1356, 3552
Psp124BI GAGCTC 2 cut(s) 618, 2965
Psp6I CCWGG 4 cut(s) 128, 1107, 1944, 4219
PspFI CCCAGC 3 cut(s) 1200, 3396, 3483
PspGI CCWGG 4 cut(s) 128, 1107, 1944, 4219
PspN4I GGNNCC 8 cut(s) 1206, 1545, 1943, 2677, 2802, 3402, 3741, 4012
PspPI GGNCC 8 cut(s) 33, 113, 1544, 1663, 1775, 1880, 3740, 3859
PstI CTGCAG 3 cut(s) 674, 2603, 2861
PstNI CAGNNNCTG 1 cut(s) 3228
PsuI RGATCY 1 cut(s) 1941
PvuII CAGCTG 9 cut(s) 523, 1655, 1898, 1960, 2604, 2856, 3225, 3851, 4051
RsaI GTAC 5 cut(s) 659, 2497, 2677, 2846, 4753
RsaNI GTAC 5 cut(s) 658, 2496, 2676, 2845, 4752
SacI GAGCTC 2 cut(s) 618, 2965
SacII CCGCGG 1 cut(s) 403
Sau96I GGNCC 8 cut(s) 33, 113, 1544, 1663, 1775, 1880, 3740, 3859
SchI GAGTC 3 cut(s) 1796, 1901, 4027
SfcI CTRYAG 8 cut(s) 502, 670, 1327, 1689, 1794, 2599, 2857, 3885
Sfr303I CCGCGG 1 cut(s) 403
SgrBI CCGCGG 1 cut(s) 403
SinI GGWCC 3 cut(s) 113, 1544, 3740
SmlI CTYRAG 5 cut(s) 617, 1379, 2964, 3211, 3949
SmoI CTYRAG 5 cut(s) 617, 1379, 2964, 3211, 3949
SphI GCATGC 2 cut(s) 2327, 4418
SseBI AGGCCT 2 cut(s) 2383, 4694
SspI AATATT 2 cut(s) 1639, 3835
SspMI CTAG 7 cut(s) 464, 1023, 1056, 1257, 2654, 3252, 4479
SstI GAGCTC 2 cut(s) 618, 2965
StuI AGGCCT 2 cut(s) 2383, 4694
StyI CCWWGG 5 cut(s) 1091, 1341, 2805, 3287, 4199
TaiI ACGT 5 cut(s) 1556, 1986, 2431, 2791, 4077
TatI WGTACW 2 cut(s) 657, 2844
TauI GCSGC 5 cut(s) 23, 52, 145, 402, 405
TseFI GTSAC 7 cut(s) 1748, 2365, 2666, 2789, 2828, 3944, 4679
Tsp45I GTSAC 7 cut(s) 1748, 2365, 2666, 2789, 2828, 3944, 4679
TspGWI ACGGA 6 cut(s) 111, 519, 1588, 2264, 2847, 4355
Van91I CCANNNNNTGG 2 cut(s) 1097, 3293
Vha464I CTTAAG 1 cut(s) 3211
VneI GTGCAC 2 cut(s) 301, 4610
VpaK11BI GGWCC 3 cut(s) 113, 1544, 3740
XagI CCTNNNNNAGG 1 cut(s) 133
XapI RAATTY 9 cut(s) 5, 81, 1409, 1442, 1991, 2093, 3638, 4082, 4184
XbaI TCTAGA 4 cut(s) 463, 1055, 2653, 3251
XceI RCATGY 7 cut(s) 972, 2027, 2327, 3165, 4094, 4118, 4418
XcmI CCANNNNNNNNNTGG 4 cut(s) 1215, 1328, 3411, 3524
XmiI GTMKAC 3 cut(s) 1472, 2530, 3668
XmnI GAANNNNTTC 2 cut(s) 2256, 4347
XspI CTAG 7 cut(s) 464, 1023, 1056, 1257, 2654, 3252, 4479
Zsp2I ATGCAT 1 cut(s) 3074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.