RchiOBHm_Chr1g0346431

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000280
Physical Location & Seq
Reverse (-)
38807550 .. 38827913
20364 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57270

Sequence Viewer

Length: 3420 bp
ATGGGGAGAGGTAGAAAGCGGTCGAGGAAAATCGCAGAGTATGGGGGTGAGGGCATTTTGGGAAATCAAAATTATAGGACCAAACGGGAGGGAGGTGAAGGGAGTTGGGATTCTCCGAGCTTGAGGAAGAGGAAGAGAGAAGGTGAAGCTGGAGGGTCATGTTCTAAGAAGAAATGTACCAAGGGCTCAAATTGGGTTAATTGGAAGTCTTCAATGTGCCATCAGTGTCAGAGGAATGATAGGGCCAGAGTTGTTAACTGCACAAGTTGTAAGCGGAAGCGATTTTGTGTATCATGCATTCAGAATTGGTATCCCCAGATGTCAGAGGAGGCTATTGCTGATGCTTGTCCTGTATGCCTTGGAAATTGCAATTGCAAAGCGTGCTTGCGTTTAGATGAAACTAATCATAATTTGAATAGCAAGCTTGCATTGCACGTTAGCAAAGATGAAGAAGTGGAGCACTCTAAGTATTTGCTTGAAGCATTGCTTCCATTCTTGAAAAGACTTAATGATGAACAAGTTATAGAGATGGTGATGGAGACTAGAAGACAAGGGAAATCTCTTTCGGAGCTAAAGATCCAAAGGTCGGACAGCTCTCCTGATGATCGTGTATACTGCGACAACTGCAATACATCTATTATTGATTTCCACAGAAGCTGTCCTATATGTTCATATGACCTTTGTCTTATCTGCTGCCGGGAGATTCGAGATGGAAACCAGCAAGGAAGTGCAGAAGAAGTGATTGGGGAGTATATCAATCGAGGTCTAGACTATTTACATGGTGGAAAGGGCAACTTAATAAAAAAGCCTTCTGAAACTAAACTCAAGTGTCCTGTAAGGTCAACATCTAAGTGGAAAGTAAGCAAAGATCGAAGTATTTTTTGCCCCTCAAAAGACATGGACGGCTGTGGTTGTAGTGTTCTAGAACTGAGATGCATATTTTCTGAAAACCACATCATAGAGTTGGTAGAAAAAGCAGAAGAAATTGCAGAAACTAAAAAACTGATGTATGCAGCTGGACCTTCTGCACAGAAGTTTTCATGGTTCAACTCTGCAGGTGGTGCTGACTCAAGAAGCAATGAATTGACGAAAGCAGCTTCTCGAGAGGATTCGGAGGACAACTACTTGTACAGTCCAAGGGCTATAAATATCCAACATGAAGACTTTAAGCATTTTCGCTGTCATTGGATCCAGGGTGAGCCTGTGATTGTGGGGAATGTGCTTGAAACTGCAACAGGTTTGAGCTGGGAACCATTTGTCATGTGGCGTGCTTGTCGTCAGATGAGAAATATCGGGCATGATAGACACGTGGAATTTAAGGCCATTGATTGCTTGGATTGGTGTGAGCTAGATATTGGTATCCACCAATTTTTTACTGGATATTTGGAGGGCCAGTTTGATGGGAAAATGTGGCCTCGTATTCTGAAACTCAACGATCAGTCCCTTGATGATTATTTGGAGAAGCGCCTTCCTCGTCATTGTGCTGAGTTTATTTGTTGCTTGCCATTTAAGGAATATACCCATCCTCGCAGTGGCCTCCTGAACCTTGCTGTAAAATTGCCTATGGAGTGTGTAAAGCCAGACATGGGGCCAAAAATGGATATTGCTTATGGTGTGTCCCAGGAGCTTGGACGTGGAGATTCAGTAACTAAGCTTCACTGTGATTCATGTGATGTGGTGAATGTTTTGACACATACTGCTGAAATGACGATTACTGCTGAGCAACTTTGTACTATAGACGGGTTGAAAAAAGAGCACATTGAACAAGACCGAAGGGAAATGTTTGAAAATTATGAGACTGTGGATGACAATGTTGATCATAAGGAGTCTGGCAGTGGGTCTAGTGTCAGGACTGCAAAGGGTAAACAATGTTGTACTCAAGTTAAGAATCAAAACAGGGTTGCAATTTTTCAAGAAAAGACTAAAGCTCAAACTGATAGTTGGAATGGTGGAAATTTTTCAAAGGAATCCATGTCTGAAAAGAATGCAGAGGGAGGTGCTTTATGGGACATTTTTCGTCGACAGGATGTTCCTAAGCTGCAGGAATATCTATGGAAACACTACAAGGAATTCAGGCACACCTACTGTTGTCCATTGCAGCAGGTCACTCACCCCATACATGATCAGACTTTTTATCTGACATTAGAGCACAAGAGGAAGCTGAAGGAAGAATATGGAATTGAACCATGGACATTTATCCAGAAACTTGGAGATGCTGTCTTTATTCCTGCAGGATGTCCACACCAAGTTAGAAACTTGAAGTCGTGCATTAAAGTTTCTGTTGAATTTGTCTCCCCTGAAAATGTTGGTGAGTGCTTACGTCTGACAGATGAGTTCCGTACACTTCCACAAAAGCATATAGCTAAAGAGGACAAATTGGAGGTGAAGAAAATGATTGTTCATGCTGTACATGAGGCTGTGGAGATTTTGGATCAGAATTTGAGGTTATGTAGCATTAAAAACTTCAGGGGAGAGAAGCCTTCACAGCCCCCTGTTAATATATGTAAGGGCAACATTGCAAACAAATTGAGAGAGAAAGCAGCTAGAGGACGACCACAAGAAGAATCGAAAGAGGTTCAAATTCTTGAGGAGCAGCCTTCACAATTATCACAGCCTATAGAGCCTGCTATTGATGTTGGTAGATTTGGATGCACACGGAGAGAGGAAAAAGATGGAGGGCAACCACAAGAAGCAAGTAGAGTAGATGAAGAGGTCCAAATTCTTAAGGGGCAGCCTTCACAGTCAGCACAACATGTACAGCCCACTGCTTATGTTGATAGCAAGCATACGATGAGAAAGGAAACAGAGGGAGGGCTACTGCAAGAAACACATGGAGAAGTTGAAGACGTCCAAATTCTTGAGAAGCAGCCTTGGCAATCATCACAAACTGTACAAGCCACTCTTCATGTTGGTAGCATTGAACATGCATCTAGAGAGGAAAAAGCTGGATGGCAACCACAAGAAACAAGTGGAGAAGATGAAGAAGTCCAAATTCTTGAGGAGCAGCATTGGCAATCATCAAAATCTGTACAAGAAACTGTTCACATTGGTAGCATTGGACATGCAATGAGAGAGGAAAAAGTGGGAGGACGACAGCATGAAGGAAGGGGAGAACAGGAAGAGGTCCGAAATCTTGGTGAAAAGCCTTCAAAGTTATCACAACCTGAACCTGTCCAGCCCACTGTTGATGTTGATAAAGCGGTGCAGCAACTGGATGAGCTCGTGGAGCTCCTTAGACATTTTGAACAGAGGAGGATACCGATGGGAGGGAGAGATGGAAGAATCAGTTCTCAATTCCAGCTTTTATGCTCGGAGTTCTTGACTTTATCTTTGCATCTTGGTTATACACCTAATGATACCAAACCTCACACTACACCATCTGCTCAGCCGCTACATGATCCTCCAGTTGTAGATCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1139

Amino Acids

129.66

Weight (kDa)

6.3

Isoelectric Point (pI)

57.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjC PF02373 660 - 763 2.5e-14 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1046
AatII GACGTC 1 cut(s) 2850
Acc36I ACCTGC 2 cut(s) 1046, 2092
AccI GTMKAC 2 cut(s) 612, 2020
AciI CCGC 4 cut(s) 19, 274, 3200, 3389
AclWI GGATC 5 cut(s) 571, 1183, 1196, 2439, 3392
AcsI RAATTY 9 cut(s) 1313, 1954, 2069, 2285, 2437, 2580, 2718, 2853, 2991
AcuI CTGAAG 2 cut(s) 2183, 2449
AcvI CACGTG 1 cut(s) 1309
AcyI GRCGYC 1 cut(s) 2847
AfaI GTAC 9 cut(s) 178, 1130, 1732, 1876, 2341, 2409, 2757, 2892, 3030
AfiI CCNNNNNNNGG 4 cut(s) 586, 1532, 1586, 3266
AflII CTTAAG 1 cut(s) 2723
AflIII ACRYGT 2 cut(s) 1306, 2752
AjiI CACGTC 1 cut(s) 1634
AjnI CCWGG 2 cut(s) 1191, 1620
AjuI GAANNNNNNNTTGG 2 cut(s) 726, 758
Alw21I GWGCWC 5 cut(s) 462, 1758, 2151, 3222, 3231
Alw26I GTCTC 3 cut(s) 533, 1790, 2296
AlwI GGATC 5 cut(s) 571, 1183, 1196, 2439, 3392
AlwNI CAGNNNCTG 2 cut(s) 657, 3211
Ama87I CYCGRG 1 cut(s) 1101
AoxI GGCC 6 cut(s) 243, 1320, 1390, 1412, 1534, 1589
ApoI RAATTY 9 cut(s) 1313, 1954, 2069, 2285, 2437, 2580, 2718, 2853, 2991
ArsI GACNNNNNNTTYG 2 cut(s) 3313, 3345
Asp700I GAANNNNTTC 3 cut(s) 1957, 3039, 3286
AspLEI GCGC 1 cut(s) 1467
AspS9I GGNCC 7 cut(s) 78, 243, 1019, 1390, 1589, 2713, 3124
AsuC2I CCSGG 1 cut(s) 698
AvaI CYCGRG 1 cut(s) 1101
AvaII GGWCC 4 cut(s) 78, 1019, 2713, 3124
BamHI GGATCC 1 cut(s) 1188
BanII GRGCYC 3 cut(s) 188, 3222, 3231
BarI GAAGNNNNNNTAC 2 cut(s) 1638, 1670
BauI CACGAG 1 cut(s) 3221
BbrPI CACGTG 1 cut(s) 1309
BbsI GAAGAC 4 cut(s) 201, 553, 1167, 2850
Bbv12I GWGCWC 5 cut(s) 462, 1758, 2151, 3222, 3231
BceAI ACGGC 1 cut(s) 919
BciT130I CCWGG 2 cut(s) 1193, 1622
BciVI GTATCC 3 cut(s) 321, 1370, 3249
BclI TGATCA 2 cut(s) 1816, 2122
BcnI CCSGG 1 cut(s) 698
BcoDI GTCTC 3 cut(s) 533, 1790, 2296
BfaI CTAG 7 cut(s) 543, 767, 923, 1349, 1842, 2544, 2931
BfmI CTRYAG 5 cut(s) 1053, 1734, 2039, 2229, 2616
BfoI RGCGCY 1 cut(s) 1468
BfrI CTTAAG 1 cut(s) 2723
BfuAI ACCTGC 2 cut(s) 1046, 2092
BfuI GTATCC 3 cut(s) 321, 1370, 3249
BglII AGATCT 1 cut(s) 3412
BlpI GCTNAGC 2 cut(s) 1719, 3384
Bme1390I CCNGG 3 cut(s) 698, 1193, 1622
Bme18I GGWCC 4 cut(s) 78, 1019, 2713, 3124
BmeT110I CYCGRG 1 cut(s) 1101
BmgBI CACGTC 1 cut(s) 1634
BmgT120I GGNCC 7 cut(s) 78, 243, 1019, 1390, 1589, 2713, 3124
BmiI GGNNCC 3 cut(s) 1190, 1251, 1590
BmrFI CCNGG 3 cut(s) 698, 1193, 1622
BmsI GCATC 6 cut(s) 331, 923, 2203, 2639, 2936, 3343
BoxI GACNNNNGTC 1 cut(s) 681
BpiI GAAGAC 4 cut(s) 201, 553, 1167, 2850
BpmI CTGGAG 2 cut(s) 171, 3387
Bpu10I CCTNAGC 1 cut(s) 2034
Bpu1102I GCTNAGC 2 cut(s) 1719, 3384
BpuEI CTTGAG 7 cut(s) 142, 809, 1054, 1863, 2606, 2879, 3017
BpuMI CCSGG 1 cut(s) 698
BsaAI YACGTR 1 cut(s) 1309
BsaHI GRCGYC 1 cut(s) 2847
BsaJI CCNNGG 7 cut(s) 180, 358, 1136, 1192, 1620, 2186, 2870
BsaXI ACNNNNNCTCC 6 cut(s) 1379, 1409, 1629, 1659, 2202, 2232
Bsc4I CCNNNNNNNGG 4 cut(s) 586, 1532, 1586, 3266
Bse1I ACTGG 4 cut(s) 1381, 1393, 3216, 3404
Bse3DI GCAATG 6 cut(s) 428, 482, 1084, 2093, 2514, 3072
BseBI CCWGG 2 cut(s) 1193, 1622
BseDI CCNNGG 7 cut(s) 180, 358, 1136, 1192, 1620, 2186, 2870
BseGI GGATG 7 cut(s) 1522, 1810, 2032, 2240, 2654, 2954, 3220
BseLI CCNNNNNNNGG 4 cut(s) 586, 1532, 1586, 3266
BseMI GCAATG 6 cut(s) 428, 482, 1084, 2093, 2514, 3072
BseMII CTCAG 4 cut(s) 920, 1476, 1710, 3398
BseNI ACTGG 4 cut(s) 1381, 1393, 3216, 3404
BseRI GAGGAG 4 cut(s) 341, 2603, 3014, 3265
BseYI CCCAGC 1 cut(s) 1245
BsgI GTGCAG 4 cut(s) 244, 750, 1011, 3224
Bsh1285I CGRYCG 1 cut(s) 23
BshFI GGCC 6 cut(s) 245, 1322, 1392, 1414, 1536, 1591
BsiEI CGRYCG 1 cut(s) 23
BsiHKAI GWGCWC 5 cut(s) 462, 1758, 2151, 3222, 3231
BsiHKCI CYCGRG 1 cut(s) 1101
BsiSI CCGG 1 cut(s) 697
BslFI GGGAC 3 cut(s) 1426, 1603, 2021
BslI CCNNNNNNNGG 4 cut(s) 586, 1532, 1586, 3266
BsmAI GTCTC 3 cut(s) 533, 1790, 2296
BsmFI GGGAC 3 cut(s) 1426, 1603, 2021
BsmI GAATGC 2 cut(s) 297, 1990
BsnI GGCC 6 cut(s) 245, 1322, 1392, 1414, 1536, 1591
BsoBI CYCGRG 1 cut(s) 1101
Bsp1286I GDGCHC 6 cut(s) 188, 462, 1758, 2151, 3222, 3231
Bsp1407I TGTACA 5 cut(s) 1128, 2407, 2755, 2890, 3028
Bsp1720I GCTNAGC 2 cut(s) 1719, 3384
Bsp19I CCATGG 1 cut(s) 2186
BspACI CCGC 4 cut(s) 19, 274, 3200, 3389
BspANI GGCC 6 cut(s) 245, 1322, 1392, 1414, 1536, 1591
BspCNI CTCAG 4 cut(s) 921, 1477, 1711, 3397
BspLI GGNNCC 3 cut(s) 1190, 1251, 1590
BspMAI CTGCAG 3 cut(s) 1057, 2043, 2233
BspMI ACCTGC 2 cut(s) 1046, 2092
BspPI GGATC 5 cut(s) 571, 1183, 1196, 2439, 3392
BspTI CTTAAG 1 cut(s) 2723
BsrDI GCAATG 6 cut(s) 428, 482, 1084, 2093, 2514, 3072
BsrGI TGTACA 5 cut(s) 1128, 2407, 2755, 2890, 3028
BsrI ACTGG 4 cut(s) 1381, 1393, 3216, 3404
BssECI CCNNGG 7 cut(s) 180, 358, 1136, 1192, 1620, 2186, 2870
BssNAI GTATAC 1 cut(s) 613
BssNI GRCGYC 1 cut(s) 2847
BssSI CACGAG 1 cut(s) 3221
BssT1I CCWWGG 5 cut(s) 180, 358, 1136, 2186, 2870
Bst1107I GTATAC 1 cut(s) 613
Bst2BI CACGAG 1 cut(s) 3221
Bst2UI CCWGG 2 cut(s) 1193, 1622
Bst4CI ACNGT 8 cut(s) 1133, 1661, 1801, 2087, 2742, 2890, 3040, 3184
Bst6I CTCTTC 5 cut(s) 122, 128, 2703, 2907, 3114
BstACI GRCGYC 1 cut(s) 2847
BstAFI CTTAAG 1 cut(s) 2723
BstAPI GCANNNNNTGC 2 cut(s) 381, 1061
BstAUI TGTACA 5 cut(s) 1128, 2407, 2755, 2890, 3028
BstBAI YACGTR 1 cut(s) 1309
BstC8I GCNNGC 8 cut(s) 382, 386, 422, 426, 1269, 1502, 2625, 2783
BstDSI CCRYGG 1 cut(s) 2186
BstF5I GGATG 7 cut(s) 1522, 1810, 2032, 2240, 2654, 2954, 3220
BstH2I RGCGCY 1 cut(s) 1468
BstHHI GCGC 1 cut(s) 1467
BstMAI GTCTC 3 cut(s) 533, 1790, 2296
BstMCI CGRYCG 1 cut(s) 23
BstMWI GCNNNNNNNGC 9 cut(s) 381, 430, 624, 1061, 2485, 2620, 2872, 3010, 3226
BstNI CCWGG 2 cut(s) 1193, 1622
BstNSI RCATGY 3 cut(s) 2756, 2927, 3065
BstPAI GACNNNNGTC 1 cut(s) 681
BstSCI CCNGG 3 cut(s) 696, 1191, 1620
BstSFI CTRYAG 5 cut(s) 1053, 1734, 2039, 2229, 2616
BstV2I GAAGAC 4 cut(s) 201, 553, 1167, 2850
BstX2I RGATCY 3 cut(s) 576, 1188, 3412
BstXI CCANNNNNNTGG 3 cut(s) 1400, 1628, 2207
BstYI RGATCY 3 cut(s) 576, 1188, 3412
BstZ17I GTATAC 1 cut(s) 613
BsuI GTATCC 3 cut(s) 321, 1370, 3249
BsuRI GGCC 6 cut(s) 245, 1322, 1392, 1414, 1536, 1591
BtgI CCRYGG 1 cut(s) 2186
BtrI CACGTC 1 cut(s) 1634
BtsCI GGATG 7 cut(s) 1522, 1810, 2032, 2240, 2654, 2954, 3220
BtsI GCAGTG 3 cut(s) 1537, 1840, 2763
BtsIMutI CAGTG 6 cut(s) 230, 1537, 1657, 1840, 2763, 3180
BveI ACCTGC 2 cut(s) 1046, 2092
Cac8I GCNNGC 8 cut(s) 382, 386, 422, 426, 1269, 1502, 2625, 2783
CaiI CAGNNNCTG 2 cut(s) 657, 3211
CfoI GCGC 1 cut(s) 1467
Cfr13I GGNCC 7 cut(s) 78, 243, 1019, 1390, 1589, 2713, 3124
Csp6I GTAC 9 cut(s) 177, 1129, 1731, 1875, 2340, 2408, 2756, 2891, 3029
CviQI GTAC 9 cut(s) 177, 1129, 1731, 1875, 2340, 2408, 2756, 2891, 3029
Eam1104I CTCTTC 5 cut(s) 122, 128, 2703, 2907, 3114
EarI CTCTTC 5 cut(s) 122, 128, 2703, 2907, 3114
Ecl136II GAGCTC 2 cut(s) 3220, 3229
Eco130I CCWWGG 5 cut(s) 180, 358, 1136, 2186, 2870
Eco24I GRGCYC 3 cut(s) 188, 3222, 3231
Eco47I GGWCC 4 cut(s) 78, 1019, 2713, 3124
Eco53kI GAGCTC 2 cut(s) 3220, 3229
Eco57I CTGAAG 2 cut(s) 2183, 2449
Eco72I CACGTG 1 cut(s) 1309
Eco88I CYCGRG 1 cut(s) 1101
EcoICRI GAGCTC 2 cut(s) 3220, 3229
EcoRI GAATTC 1 cut(s) 2069
EcoRII CCWGG 2 cut(s) 1191, 1620
EcoT14I CCWWGG 5 cut(s) 180, 358, 1136, 2186, 2870
EcoT22I ATGCAT 3 cut(s) 299, 938, 2929
EcoT38I GRGCYC 3 cut(s) 188, 3222, 3231
ErhI CCWWGG 5 cut(s) 180, 358, 1136, 2186, 2870
FalI AAGNNNNNCTT 6 cut(s) 471, 503, 779, 811, 2886, 2918
FaqI GGGAC 3 cut(s) 1426, 1603, 2021
FauNDI CATATG 1 cut(s) 673
FbaI TGATCA 2 cut(s) 1816, 2122
FblI GTMKAC 2 cut(s) 612, 2020
FokI GGATG 7 cut(s) 1509, 1817, 2039, 2247, 2661, 2961, 3227
FriOI GRGCYC 3 cut(s) 188, 3222, 3231
FspBI CTAG 7 cut(s) 543, 767, 923, 1349, 1842, 2544, 2931
GlaI GCGC 1 cut(s) 1466
GsaI CCCAGC 1 cut(s) 1249
GsuI CTGGAG 2 cut(s) 171, 3387
HaeII RGCGCY 1 cut(s) 1468
HaeIII GGCC 6 cut(s) 245, 1322, 1392, 1414, 1536, 1591
HapII CCGG 1 cut(s) 697
HhaI GCGC 1 cut(s) 1467
Hin1I GRCGYC 1 cut(s) 2847
Hin6I GCGC 1 cut(s) 1465
HinP1I GCGC 1 cut(s) 1465
HincII GTYRAC 3 cut(s) 256, 843, 2021
HindII GTYRAC 3 cut(s) 256, 843, 2021
HindIII AAGCTT 2 cut(s) 422, 1652
HpaI GTTAAC 1 cut(s) 256
HpaII CCGG 1 cut(s) 697
Hpy166II GTNNAC 8 cut(s) 256, 613, 843, 1865, 2021, 2240, 2342, 3043
Hpy8I GTNNAC 8 cut(s) 256, 613, 843, 1865, 2021, 2240, 2342, 3043
Hpy99I CGWCG 1 cut(s) 2022
HpyCH4III ACNGT 8 cut(s) 1133, 1661, 1801, 2087, 2742, 2890, 3040, 3184
HpyCH4IV ACGT 5 cut(s) 435, 1308, 1633, 2320, 2847
HpyF10VI GCNNNNNNNGC 9 cut(s) 381, 430, 624, 1061, 2485, 2620, 2872, 3010, 3226
HpySE526I ACGT 5 cut(s) 435, 1308, 1633, 2320, 2847
Hsp92I GRCGYC 1 cut(s) 2847
HspAI GCGC 1 cut(s) 1465
Ksp22I TGATCA 2 cut(s) 1816, 2122
KspAI GTTAAC 1 cut(s) 256
LmnI GCTCC 7 cut(s) 457, 568, 1624, 2590, 3001, 3226, 3234
LweI GCATC 6 cut(s) 331, 923, 2203, 2639, 2936, 3343
MaeI CTAG 7 cut(s) 543, 767, 923, 1349, 1842, 2544, 2931
MaeII ACGT 5 cut(s) 435, 1308, 1633, 2320, 2847
MaeIII GTNAC 2 cut(s) 1645, 2104
MfeI CAATTG 1 cut(s) 370
MflI RGATCY 3 cut(s) 576, 1188, 3412
MhlI GDGCHC 6 cut(s) 188, 462, 1758, 2151, 3222, 3231
MlyI GAGTC 2 cut(s) 1061, 1835
MmeI TCCRAC 3 cut(s) 567, 1177, 1922
Mph1103I ATGCAT 3 cut(s) 299, 938, 2929
MroXI GAANNNNTTC 3 cut(s) 1957, 3039, 3286
MslI CAYNNNNRTG 1 cut(s) 850
MspA1I CMGCKG 1 cut(s) 1016
MspCI CTTAAG 1 cut(s) 2723
MspI CCGG 1 cut(s) 697
MspR9I CCNGG 3 cut(s) 698, 1193, 1622
MunI CAATTG 1 cut(s) 370
Mva1269I GAATGC 2 cut(s) 297, 1990
MvaI CCWGG 2 cut(s) 1193, 1622
MwoI GCNNNNNNNGC 9 cut(s) 381, 430, 624, 1061, 2485, 2620, 2872, 3010, 3226
NciI CCSGG 1 cut(s) 698
NcoI CCATGG 1 cut(s) 2186
NdeI CATATG 1 cut(s) 673
NlaIV GGNNCC 3 cut(s) 1190, 1251, 1590
NmuCI GTSAC 1 cut(s) 2104
NsiI ATGCAT 3 cut(s) 299, 938, 2929
NspI RCATGY 3 cut(s) 2756, 2927, 3065
PaeR7I CTCGAG 1 cut(s) 1101
PaqCI CACCTGC 1 cut(s) 1046
PciI ACATGT 1 cut(s) 2752
PctI GAATGC 2 cut(s) 297, 1990
PdmI GAANNNNTTC 3 cut(s) 1957, 3039, 3286
PfeI GAWTC 9 cut(s) 110, 703, 1109, 1640, 1664, 1888, 1967, 2564, 3282
PleI GAGTC 2 cut(s) 1061, 1834
PmaCI CACGTG 1 cut(s) 1309
PmlI CACGTG 1 cut(s) 1309
PpsI GAGTC 2 cut(s) 1061, 1834
Ppu21I YACGTR 1 cut(s) 1309
PscI ACATGT 1 cut(s) 2752
PshAI GACNNNNGTC 1 cut(s) 681
Psp124BI GAGCTC 2 cut(s) 3222, 3231
Psp6I CCWGG 2 cut(s) 1191, 1620
PspCI CACGTG 1 cut(s) 1309
PspFI CCCAGC 1 cut(s) 1245
PspGI CCWGG 2 cut(s) 1191, 1620
PspN4I GGNNCC 3 cut(s) 1190, 1251, 1590
PspPI GGNCC 7 cut(s) 78, 243, 1019, 1390, 1589, 2713, 3124
PstI CTGCAG 3 cut(s) 1057, 2043, 2233
PstNI CAGNNNCTG 2 cut(s) 657, 3211
PsuI RGATCY 3 cut(s) 576, 1188, 3412
PvuII CAGCTG 1 cut(s) 1016
RsaI GTAC 9 cut(s) 178, 1130, 1732, 1876, 2341, 2409, 2757, 2892, 3030
RsaNI GTAC 9 cut(s) 177, 1129, 1731, 1875, 2340, 2408, 2756, 2891, 3029
RseI CAYNNNNRTG 1 cut(s) 850
SacI GAGCTC 2 cut(s) 3222, 3231
SalI GTCGAC 1 cut(s) 2019
Sau96I GGNCC 7 cut(s) 78, 243, 1019, 1390, 1589, 2713, 3124
SbfI CCTGCAGG 1 cut(s) 2233
SchI GAGTC 2 cut(s) 1061, 1835
ScrFI CCNGG 3 cut(s) 698, 1193, 1622
SdaI CCTGCAGG 1 cut(s) 2233
SduI GDGCHC 6 cut(s) 188, 462, 1758, 2151, 3222, 3231
SfaNI GCATC 6 cut(s) 331, 923, 2203, 2639, 2936, 3343
SfcI CTRYAG 5 cut(s) 1053, 1734, 2039, 2229, 2616
Sfr274I CTCGAG 1 cut(s) 1101
SinI GGWCC 4 cut(s) 78, 1019, 2713, 3124
SlaI CTCGAG 1 cut(s) 1101
SmiMI CAYNNNNRTG 1 cut(s) 850
SmlI CTYRAG 9 cut(s) 121, 824, 1069, 1101, 1878, 2585, 2723, 2858, 2996
SmoI CTYRAG 9 cut(s) 121, 824, 1069, 1101, 1878, 2585, 2723, 2858, 2996
Sse8387I CCTGCAGG 1 cut(s) 2233
SsiI CCGC 4 cut(s) 19, 274, 3200, 3389
SspMI CTAG 7 cut(s) 543, 767, 923, 1349, 1842, 2544, 2931
SstI GAGCTC 2 cut(s) 3222, 3231
StyD4I CCNGG 3 cut(s) 696, 1191, 1620
StyI CCWWGG 5 cut(s) 180, 358, 1136, 2186, 2870
TaaI ACNGT 8 cut(s) 1133, 1661, 1801, 2087, 2742, 2890, 3040, 3184
TaiI ACGT 5 cut(s) 438, 1311, 1636, 2323, 2850
TaqI TCGA 7 cut(s) 23, 706, 760, 871, 1102, 2020, 2567
TaqII GACCGA 1 cut(s) 1785
TatI WGTACW 7 cut(s) 1128, 1730, 1874, 2407, 2755, 2890, 3028
TauI GCSGC 1 cut(s) 3391
TfiI GAWTC 9 cut(s) 110, 703, 1109, 1640, 1664, 1888, 1967, 2564, 3282
TscAI CASTG 6 cut(s) 230, 1537, 1664, 1840, 2770, 3187
TseFI GTSAC 1 cut(s) 2104
Tsp45I GTSAC 1 cut(s) 2104
TspGWI ACGGA 2 cut(s) 2327, 2671
TspRI CASTG 6 cut(s) 230, 1537, 1664, 1840, 2770, 3187
Vha464I CTTAAG 1 cut(s) 2723
VpaK11BI GGWCC 4 cut(s) 78, 1019, 2713, 3124
XapI RAATTY 9 cut(s) 1313, 1954, 2069, 2285, 2437, 2580, 2718, 2853, 2991
XbaI TCTAGA 3 cut(s) 766, 922, 2930
XceI RCATGY 3 cut(s) 2756, 2927, 3065
XcmI CCANNNNNNNNNTGG 4 cut(s) 1260, 1330, 1373, 1529
XhoI CTCGAG 1 cut(s) 1101
XmiI GTMKAC 2 cut(s) 612, 2020
XmnI GAANNNNTTC 3 cut(s) 1957, 3039, 3286
XspI CTAG 7 cut(s) 543, 767, 923, 1349, 1842, 2544, 2931
ZraI GACGTC 1 cut(s) 2848
Zsp2I ATGCAT 3 cut(s) 299, 938, 2929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.