RchiOBHm_Chr1g0346441

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000280
Physical Location & Seq
Reverse (-)
38828715 .. 38837417
8703 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57271

Sequence Viewer

Length: 2670 bp
ATGAAGAGAGACGGCGTTAAGCAACCTTATGATGACCCAGAACCTACATTTCCAGAGAAAGACGAGGACTTTTTAGAAAACCAGAGTCACATGGCTGAGATTGTGCAGAAGAAGCGTAGTACTAGGAAGAGAGCTACAGGGAAGGGATTGGCAGCCGTGAATGGCCCAAGGGATTGTGAGAAAGGAGTTCTATCATGCACTGCCAACTGGGGTCGGAAGCGGTTTAGGCGCGTGAGACCACGCAACGCTGCAAAGTGGATTGAGGATTGTTTAGAGTGCCTTCACTGTCAGGGGAATGATGAGGGCAGAGTTGTTTGCTGTGGAAGTTGCAAAAGAAAATGGTATCCTCAGATGTTAGAGGATGCCATTGCTGAGGCTTGTCCTGGATGCTGTAGTAATTGCAGTTGCAAAGCATGTTTGTGTGCAGCTTCTAGAAATTTGAAGAAACAGCCGGAGTTGAAAATTAGCAAAGAGGAAAAACTTGAGCACTCTAAGTATTTGCTTCAAGCATTGCTTCCACTATTAAAACGACTTAACGATGAGCAACTGATTGAGATGGAGATGGAGGCTAGGAGACAAGGGCTGTCTGTTTCGGAGTTAAAGATCCAAAGGTCAAACTGCTCTTATGATGAGCGTGTGAATTGTAACAACTGCAAAGCTTCAATTGTTGATTTGTATAGAAGCTGCAATTTATGCTCATATGATCTCTGCCTAACGTGTTGCCGGGAGAGTCGAGATCGACACCTGGATGGAGGTGGGAAGGAAGTGACTATGGTGTATATCAATCAGGACCTAGAACATTTGCATGGTGGAAAGGGGAACATAGCAGAACTACTGTTTGAACCTAGCCCCAAGTGTCATGTAAGGTTAAAATCTGAATGGAAATCCAATGAAGATGTTAGCATCCTTTGCCCTCCAGAGGATAGGGATGGCTGTGGTCATGGTCATCTGGAATTGAGATGCATGTCTTTTGAAAATAAGGTCGGTGAGTTGGTACAGAAAGCAGAAGAGATGGCTGAAATTTACAAACTTAAGCATGCGGCAGAAACTTCTGCACAGTGGTGTTCATGTTTCAATTCTTTGGATGTTTTTGATTCAATCAGCAACACCAAAAAGTTAAGGAAAGGAGATTCTCAAGAGGATTTTGATGACAACTATTTGTACTGCCCAAGAGCTGGAAATATCCAACGTGAGGATCTTAAGCATTTCCAATGGCATTGGCTCAGGGGTGAGCCTGTGATTGTTAGCAATGTAATTGAAACTACATCAGGTATGTGGCCTGCTTGTCGTCAGATGCAACAGACTATGCATGGTAAATATTTAACAATCAAGGCCATTGATTGCCTGGATTGGTGCAAGCTAGAGAAGTTGAGAAGATATATTGGTACTGGAGTTGTCATCAATTCAGATATCAGTATCCATGAATTTTTTACTGGATATGCAAAGGGGTGGTTTGATTGGAAAATGGGGCCTCAAAGGCTAAAACTGAGAGATTGTGCTCCAGCGAGTTTGATTGAGGAGCACCTTCCTCATCATGGTGCTGAGTTTATCTATGGCTTGCCATTTAAGGAATATACTCATCCTCACAGTGGGTTTCTTAATCTTGCTGCTAAATTCCCTGAGGAGTGTGGGAAGCCTGCTTATGGCGTTGGGCAGGAGCTTGGACGTGAAGATTCTGTGACTAAGCTTCATTGCAATATATCTGATGTGGTGAATGTTTTGACACATACTGCTGAAGTGACCCCTAATTCTCAACAACTTCGTACAGTAGAAAGGCTAAAAAAAGCGCAGATTGAGCAAGATAGAAGAGAAACTTTAGGAAACTGTCACTCTGCGGATGGCAATGTTGATGGTGGAAATCTTTCAAACTATTCCAGATCTGAAAATAATGTAGACGGTGGTGCTTTATGGGACATTTTTCGGAGACAGGATGTTCCAAAACTACAGGAATATTTCAGGAAGCACTTCAACGAGTTCAGGCACACATACTGCTGTCCGTCCCAACAGATTATTCATCCCATTCACGATCAGAACTTTTACCTGACTCGGGAGCACAAAAGGAAGCTGAAAGAGGAATGTGGAATCGAACCATGGACATGTATCCAGAAACTTGGGGATGCTATCTTTGTTCCTGCAGGCTGTCCATATCAAGTCAGAAACTTGAAGTCATGCATTAAAGTTGCAGTTCAATTTGTCTCACCTGAAAGTGTTGGCGATTGCTTTCGTTTGACTGAAGAATGCCGCACGCTTCCAAAAGATCACGGGGCTAAAGAGGATAAATTGGAGGTGAAAAAAATGATTGTGCATGCTATGCGGGAAGCTGTGGATATTTTGGATCAGAGTGTGAGGGTTAGACAAGCAAAGAGAGAGGAAGTTGGACAAAGACAGCAAGTACAGAGCAGAAAGGAGGGGGAAGTTCAAGCTGACGAGGAGCAGCCAACAATGTCATCACATCCAGCACAGTTCCCTCTTGATCTTGTTACAGCAATGCAGCGGCTAGATGACCTCGTGGAGCGCTTTTCAGATTTTGAACACTTGGAAGAGAGAGATAGAATAATGAATCCGGAATACCAGTTCTTATGTTCAGAGTTCATGAATTTATCTTCACATCTTGGTTATGCAACTCAGGTGAAAGAGTCTGCCATTCCACCATCCTATCAGCAACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

889

Amino Acids

102.03

Weight (kDa)

6.51

Isoelectric Point (pI)

54.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjC PF02373 632 - 731 8.7e-13 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1175
AccI GTMKAC 1 cut(s) 1893
AccII CGCG 1 cut(s) 231
AccIII TCCGGA 1 cut(s) 2563
AciI CCGC 6 cut(s) 220, 1040, 1835, 2242, 2314, 2494
AclWI GGATC 3 cut(s) 598, 1203, 2343
AcsI RAATTY 5 cut(s) 436, 1020, 1424, 1613, 2596
AcuI CTGAAG 2 cut(s) 1755, 2253
AfaI GTAC 6 cut(s) 121, 996, 1163, 1387, 1765, 2394
AfeI AGCGCT 1 cut(s) 2516
AfiI CCNNNNNNNGG 7 cut(s) 919, 1175, 1192, 1535, 1589, 1643, 2047
AflII CTTAAG 2 cut(s) 1031, 1199
AflIII ACRYGT 2 cut(s) 716, 2096
AjiI CACGTC 1 cut(s) 1667
AjnI CCWGG 3 cut(s) 382, 744, 1344
AjuI GAANNNNNNNTTGG 2 cut(s) 1995, 2027
AleI CACNNNNGTG 1 cut(s) 1060
AloI GAACNNNNNNTCC 2 cut(s) 2558, 2590
Alw21I GWGCWC 4 cut(s) 489, 1501, 1524, 2055
Alw26I GTCTC 5 cut(s) 3, 229, 568, 1918, 2200
AlwI GGATC 3 cut(s) 598, 1203, 2343
Ama87I CYCGRG 1 cut(s) 2046
Aor13HI TCCGGA 1 cut(s) 2563
Aor51HI AGCGCT 1 cut(s) 2516
AoxI GGCC 4 cut(s) 163, 1277, 1332, 1469
ApeKI GCWGC 7 cut(s) 152, 248, 425, 684, 1607, 2434, 2491
ApoI RAATTY 5 cut(s) 436, 1020, 1424, 1613, 2596
Asp700I GAANNNNTTC 2 cut(s) 1861, 1964
AspLEI GCGC 3 cut(s) 231, 1789, 2517
AspS9I GGNCC 3 cut(s) 164, 790, 1469
AsuC2I CCSGG 1 cut(s) 725
AsuHPI GGTGA 6 cut(s) 998, 1241, 1723, 2190, 2299, 2641
AvaI CYCGRG 1 cut(s) 2046
AvaII GGWCC 1 cut(s) 790
AxyI CCTNAGG 1 cut(s) 1620
BaeI ACNNNNGTAYC 2 cut(s) 1377, 1410
BauI CACGAG 1 cut(s) 2507
Bbv12I GWGCWC 4 cut(s) 489, 1501, 1524, 2055
BbvCI CCTCAGC 1 cut(s) 372
BbvI GCAGC 7 cut(s) 164, 235, 437, 671, 1594, 2446, 2503
BccI CCATC 8 cut(s) 550, 556, 743, 923, 1006, 1832, 1844, 2659
BceAI ACGGC 2 cut(s) 28, 140
BciT130I CCWGG 3 cut(s) 384, 746, 1346
BciVI GTATCC 3 cut(s) 354, 1427, 2111
BcnI CCSGG 1 cut(s) 725
BcoDI GTCTC 5 cut(s) 3, 229, 568, 1918, 2200
BfaI CTAG 7 cut(s) 123, 432, 570, 794, 846, 1361, 2498
BfmI CTRYAG 4 cut(s) 135, 391, 1943, 2133
BfoI RGCGCY 1 cut(s) 2518
BfrI CTTAAG 2 cut(s) 1031, 1199
BfuI GTATCC 3 cut(s) 354, 1427, 2111
BglI GCCNNNNNGGC 1 cut(s) 1477
BglII AGATCT 1 cut(s) 1877
BmcAI AGTACT 1 cut(s) 121
Bme1390I CCNGG 4 cut(s) 384, 725, 746, 1346
Bme18I GGWCC 1 cut(s) 790
BmeT110I CYCGRG 1 cut(s) 2046
BmgBI CACGTC 1 cut(s) 1667
BmgT120I GGNCC 3 cut(s) 164, 790, 1469
BmiI GGNNCC 1 cut(s) 1470
BmrFI CCNGG 4 cut(s) 384, 725, 746, 1346
BmrI ACTGGG 1 cut(s) 217
BmsI GCATC 6 cut(s) 352, 377, 912, 950, 1284, 2107
BmuI ACTGGG 1 cut(s) 217
BpmI CTGGAG 3 cut(s) 900, 1410, 1485
Bpu10I CCTNAGC 2 cut(s) 372, 1223
BpuEI CTTGAG 2 cut(s) 503, 1119
BpuMI CCSGG 1 cut(s) 725
BsaI GGTCTC 1 cut(s) 229
BsaJI CCNNGG 2 cut(s) 167, 2090
BsaWI WCCGGW 1 cut(s) 2563
Bsc4I CCNNNNNNNGG 7 cut(s) 919, 1175, 1192, 1535, 1589, 1643, 2047
Bse1I ACTGG 4 cut(s) 212, 1393, 1438, 2572
Bse21I CCTNAGG 1 cut(s) 1620
Bse3DI GCAATG 6 cut(s) 366, 509, 1255, 1690, 1849, 2493
BseAI TCCGGA 1 cut(s) 2563
BseBI CCWGG 3 cut(s) 384, 746, 1346
BseDI CCNNGG 2 cut(s) 167, 2090
BseLI CCNNNNNNNGG 7 cut(s) 919, 1175, 1192, 1535, 1589, 1643, 2047
BseMI GCAATG 6 cut(s) 366, 509, 1255, 1690, 1849, 2493
BseMII CTCAG 8 cut(s) 87, 362, 363, 1237, 1478, 1533, 1611, 2639
BseNI ACTGG 4 cut(s) 212, 1393, 1438, 2572
BseRI GAGGAG 3 cut(s) 1532, 1637, 2444
BseXI GCAGC 7 cut(s) 164, 235, 437, 671, 1594, 2446, 2503
BsgI GTGCAG 3 cut(s) 125, 444, 1038
Bsh1236I CGCG 1 cut(s) 231
BshFI GGCC 4 cut(s) 165, 1279, 1334, 1471
BsiHKAI GWGCWC 4 cut(s) 489, 1501, 1524, 2055
BsiHKCI CYCGRG 1 cut(s) 2046
BsiSI CCGG 3 cut(s) 452, 724, 2564
BslFI GGGAC 2 cut(s) 1925, 1984
BslI CCNNNNNNNGG 7 cut(s) 919, 1175, 1192, 1535, 1589, 1643, 2047
BsmAI GTCTC 5 cut(s) 3, 229, 568, 1918, 2200
BsmBI CGTCTC 1 cut(s) 3
BsmFI GGGAC 2 cut(s) 1925, 1984
BsmI GAATGC 1 cut(s) 2243
BsnI GGCC 4 cut(s) 165, 1279, 1334, 1471
Bso31I GGTCTC 1 cut(s) 229
BsoBI CYCGRG 1 cut(s) 2046
Bsp1286I GDGCHC 4 cut(s) 489, 1501, 1524, 2055
Bsp13I TCCGGA 1 cut(s) 2563
Bsp143I GATC 9 cut(s) 603, 703, 736, 1195, 1877, 2026, 2257, 2335, 2473
Bsp19I CCATGG 1 cut(s) 2090
BspACI CCGC 6 cut(s) 220, 1040, 1835, 2242, 2314, 2494
BspANI GGCC 4 cut(s) 165, 1279, 1334, 1471
BspCNI CTCAG 8 cut(s) 88, 361, 364, 1236, 1479, 1534, 1612, 2638
BspEI TCCGGA 1 cut(s) 2563
BspFNI CGCG 1 cut(s) 231
BspHI TCATGA 1 cut(s) 2592
BspLI GGNNCC 1 cut(s) 1470
BspMAI CTGCAG 1 cut(s) 2137
BspPI GGATC 3 cut(s) 598, 1203, 2343
BspTI CTTAAG 2 cut(s) 1031, 1199
BspTNI GGTCTC 1 cut(s) 229
BsrDI GCAATG 6 cut(s) 366, 509, 1255, 1690, 1849, 2493
BsrI ACTGG 4 cut(s) 212, 1393, 1438, 2572
BssECI CCNNGG 2 cut(s) 167, 2090
BssMI GATC 9 cut(s) 603, 703, 736, 1195, 1877, 2026, 2257, 2335, 2473
BssSI CACGAG 1 cut(s) 2507
BssT1I CCWWGG 2 cut(s) 167, 2090
Bst2BI CACGAG 1 cut(s) 2507
Bst2UI CCWGG 3 cut(s) 384, 746, 1346
Bst4CI ACNGT 8 cut(s) 287, 837, 1059, 1589, 1768, 1826, 1898, 2463
Bst6I CTCTTC 4 cut(s) 122, 1002, 1801, 2535
BstAFI CTTAAG 2 cut(s) 1031, 1199
BstAPI GCANNNNNTGC 3 cut(s) 693, 909, 2311
BstC8I GCNNGC 8 cut(s) 1038, 1281, 1358, 1559, 1638, 2137, 2246, 2307
BstDSI CCRYGG 1 cut(s) 2090
BstFNI CGCG 1 cut(s) 231
BstH2I RGCGCY 1 cut(s) 2518
BstHHI GCGC 3 cut(s) 231, 1789, 2517
BstKTI GATC 9 cut(s) 606, 706, 739, 1198, 1880, 2029, 2260, 2338, 2476
BstMAI GTCTC 5 cut(s) 3, 229, 568, 1918, 2200
BstMBI GATC 9 cut(s) 603, 703, 736, 1195, 1877, 2026, 2257, 2335, 2473
BstMWI GCNNNNNNNGC 7 cut(s) 112, 226, 693, 909, 1477, 1795, 2311
BstNI CCWGG 3 cut(s) 384, 746, 1346
BstNSI RCATGY 5 cut(s) 417, 967, 1040, 2100, 2309
BstSCI CCNGG 4 cut(s) 382, 723, 744, 1344
BstSFI CTRYAG 4 cut(s) 135, 391, 1943, 2133
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 7 cut(s) 164, 235, 437, 671, 1594, 2446, 2503
BstX2I RGATCY 3 cut(s) 603, 1195, 1877
BstXI CCANNNNNNTGG 1 cut(s) 2111
BstYI RGATCY 3 cut(s) 603, 1195, 1877
Bsu36I CCTNAGG 1 cut(s) 1620
BsuI GTATCC 3 cut(s) 354, 1427, 2111
BsuRI GGCC 4 cut(s) 165, 1279, 1334, 1471
BtgI CCRYGG 1 cut(s) 2090
BtrI CACGTC 1 cut(s) 1667
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 4 cut(s) 198, 283, 1064, 1594
Cac8I GCNNGC 8 cut(s) 1038, 1281, 1358, 1559, 1638, 2137, 2246, 2307
CciI TCATGA 1 cut(s) 2592
CfoI GCGC 3 cut(s) 231, 1789, 2517
Cfr13I GGNCC 3 cut(s) 164, 790, 1469
Csp6I GTAC 6 cut(s) 120, 995, 1162, 1386, 1764, 2393
CviQI GTAC 6 cut(s) 120, 995, 1162, 1386, 1764, 2393
DpnI GATC 9 cut(s) 605, 705, 738, 1197, 1879, 2028, 2259, 2337, 2475
DpnII GATC 9 cut(s) 603, 703, 736, 1195, 1877, 2026, 2257, 2335, 2473
Eam1104I CTCTTC 4 cut(s) 122, 1002, 1801, 2535
EarI CTCTTC 4 cut(s) 122, 1002, 1801, 2535
Eco130I CCWWGG 2 cut(s) 167, 2090
Eco31I GGTCTC 1 cut(s) 229
Eco32I GATATC 1 cut(s) 1411
Eco47I GGWCC 1 cut(s) 790
Eco47III AGCGCT 1 cut(s) 2516
Eco57I CTGAAG 2 cut(s) 1755, 2253
Eco81I CCTNAGG 1 cut(s) 1620
Eco88I CYCGRG 1 cut(s) 2046
EcoO109I RGGNCCY 2 cut(s) 790, 1469
EcoRII CCWGG 3 cut(s) 382, 744, 1344
EcoRV GATATC 1 cut(s) 1411
EcoT14I CCWWGG 2 cut(s) 167, 2090
EcoT22I ATGCAT 3 cut(s) 965, 1311, 2174
ErhI CCWWGG 2 cut(s) 167, 2090
Esp3I CGTCTC 1 cut(s) 3
FalI AAGNNNNNCTT 4 cut(s) 498, 530, 1798, 1830
FaqI GGGAC 2 cut(s) 1925, 1984
FauI CCCGC 1 cut(s) 2307
FauNDI CATATG 1 cut(s) 700
FblI GTMKAC 1 cut(s) 1893
FspBI CTAG 7 cut(s) 123, 432, 570, 794, 846, 1361, 2498
GlaI GCGC 3 cut(s) 230, 1788, 2516
GsuI CTGGAG 3 cut(s) 900, 1410, 1485
HaeII RGCGCY 1 cut(s) 2518
HaeIII GGCC 4 cut(s) 165, 1279, 1334, 1471
HapII CCGG 3 cut(s) 452, 724, 2564
HhaI GCGC 3 cut(s) 231, 1789, 2517
Hin6I GCGC 3 cut(s) 229, 1787, 2515
HinP1I GCGC 3 cut(s) 229, 1787, 2515
HindIII AAGCTT 2 cut(s) 657, 1685
HinfI GANTC 9 cut(s) 85, 730, 1094, 1130, 1673, 2044, 2082, 2560, 2636
HpaII CCGG 3 cut(s) 452, 724, 2564
HphI GGTGA 6 cut(s) 998, 1241, 1723, 2190, 2299, 2641
Hpy166II GTNNAC 1 cut(s) 1894
Hpy8I GTNNAC 1 cut(s) 1894
HpyAV CCTTC 4 cut(s) 136, 290, 754, 1535
HpyCH4III ACNGT 8 cut(s) 287, 837, 1059, 1589, 1768, 1826, 1898, 2463
HpyCH4IV ACGT 3 cut(s) 716, 1189, 1666
HpyF10VI GCNNNNNNNGC 7 cut(s) 112, 226, 693, 909, 1477, 1795, 2311
HpySE526I ACGT 3 cut(s) 716, 1189, 1666
HspAI GCGC 3 cut(s) 229, 1787, 2515
Kpn2I TCCGGA 1 cut(s) 2563
Kzo9I GATC 9 cut(s) 603, 703, 736, 1195, 1877, 2026, 2257, 2335, 2473
LmnI GCTCC 6 cut(s) 1504, 1519, 1657, 2050, 2431, 2512
Lsp1109I GCAGC 7 cut(s) 164, 235, 437, 671, 1594, 2446, 2503
LweI GCATC 6 cut(s) 352, 377, 912, 950, 1284, 2107
MaeI CTAG 7 cut(s) 123, 432, 570, 794, 846, 1361, 2498
MaeII ACGT 3 cut(s) 716, 1189, 1666
MaeIII GTNAC 7 cut(s) 86, 644, 766, 1678, 1738, 1826, 2479
MalI GATC 9 cut(s) 605, 705, 738, 1197, 1879, 2028, 2259, 2337, 2475
MboI GATC 9 cut(s) 603, 703, 736, 1195, 1877, 2026, 2257, 2335, 2473
MfeI CAATTG 1 cut(s) 663
MflI RGATCY 3 cut(s) 603, 1195, 1877
MhlI GDGCHC 4 cut(s) 489, 1501, 1524, 2055
MlyI GAGTC 4 cut(s) 94, 739, 2038, 2645
MmeI TCCRAC 3 cut(s) 194, 1210, 2356
Mph1103I ATGCAT 3 cut(s) 965, 1311, 2174
MroI TCCGGA 1 cut(s) 2563
MroXI GAANNNNTTC 2 cut(s) 1861, 1964
MslI CAYNNNNRTG 6 cut(s) 418, 747, 804, 1060, 1536, 2095
MspA1I CMGCKG 1 cut(s) 2494
MspCI CTTAAG 2 cut(s) 1031, 1199
MspI CCGG 3 cut(s) 452, 724, 2564
MspR9I CCNGG 4 cut(s) 384, 725, 746, 1346
MunI CAATTG 1 cut(s) 663
Mva1269I GAATGC 1 cut(s) 2243
MvaI CCWGG 3 cut(s) 384, 746, 1346
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 7 cut(s) 112, 226, 693, 909, 1477, 1795, 2311
NciI CCSGG 1 cut(s) 725
NcoI CCATGG 1 cut(s) 2090
NdeI CATATG 1 cut(s) 700
NdeII GATC 9 cut(s) 603, 703, 736, 1195, 1877, 2026, 2257, 2335, 2473
NlaIV GGNNCC 1 cut(s) 1470
NmuCI GTSAC 5 cut(s) 86, 766, 1678, 1738, 1826
NsiI ATGCAT 3 cut(s) 965, 1311, 2174
NspI RCATGY 5 cut(s) 417, 967, 1040, 2100, 2309
OliI CACNNNNGTG 1 cut(s) 1060
PaeI GCATGC 2 cut(s) 1040, 2309
PagI TCATGA 1 cut(s) 2592
PciI ACATGT 1 cut(s) 2096
PctI GAATGC 1 cut(s) 2243
PdmI GAANNNNTTC 2 cut(s) 1861, 1964
PfeI GAWTC 5 cut(s) 1094, 1130, 1673, 2082, 2560
PflMI CCANNNNNTGG 1 cut(s) 1175
PfoI TCCNGGA 1 cut(s) 382
PleI GAGTC 4 cut(s) 93, 738, 2038, 2644
PpsI GAGTC 4 cut(s) 93, 738, 2038, 2644
PpuMI RGGWCCY 1 cut(s) 790
PscI ACATGT 1 cut(s) 2096
Psp5II RGGWCCY 1 cut(s) 790
Psp6I CCWGG 3 cut(s) 382, 744, 1344
PspGI CCWGG 3 cut(s) 382, 744, 1344
PspN4I GGNNCC 1 cut(s) 1470
PspPI GGNCC 3 cut(s) 164, 790, 1469
PspPPI RGGWCCY 1 cut(s) 790
PstI CTGCAG 1 cut(s) 2137
PsuI RGATCY 3 cut(s) 603, 1195, 1877
RsaI GTAC 6 cut(s) 121, 996, 1163, 1387, 1765, 2394
RsaNI GTAC 6 cut(s) 120, 995, 1162, 1386, 1764, 2393
RseI CAYNNNNRTG 6 cut(s) 418, 747, 804, 1060, 1536, 2095
Sau3AI GATC 9 cut(s) 603, 703, 736, 1195, 1877, 2026, 2257, 2335, 2473
Sau96I GGNCC 3 cut(s) 164, 790, 1469
SbfI CCTGCAGG 1 cut(s) 2137
ScaI AGTACT 1 cut(s) 121
SchI GAGTC 4 cut(s) 94, 739, 2038, 2645
ScrFI CCNGG 4 cut(s) 384, 725, 746, 1346
SdaI CCTGCAGG 1 cut(s) 2137
SduI GDGCHC 4 cut(s) 489, 1501, 1524, 2055
SfaNI GCATC 6 cut(s) 352, 377, 912, 950, 1284, 2107
SfcI CTRYAG 4 cut(s) 135, 391, 1943, 2133
SinI GGWCC 1 cut(s) 790
SmiMI CAYNNNNRTG 6 cut(s) 418, 747, 804, 1060, 1536, 2095
SmlI CTYRAG 4 cut(s) 482, 1031, 1134, 1199
SmoI CTYRAG 4 cut(s) 482, 1031, 1134, 1199
SphI GCATGC 2 cut(s) 1040, 2309
Sse8387I CCTGCAGG 1 cut(s) 2137
SsiI CCGC 6 cut(s) 220, 1040, 1835, 2242, 2314, 2494
SspI AATATT 2 cut(s) 1319, 1952
SspMI CTAG 7 cut(s) 123, 432, 570, 794, 846, 1361, 2498
StyD4I CCNGG 4 cut(s) 382, 723, 744, 1344
StyI CCWWGG 2 cut(s) 167, 2090
TaaI ACNGT 8 cut(s) 287, 837, 1059, 1589, 1768, 1826, 1898, 2463
TaiI ACGT 3 cut(s) 719, 1192, 1669
TaqI TCGA 3 cut(s) 733, 739, 2085
TatI WGTACW 3 cut(s) 119, 1161, 2392
TauI GCSGC 3 cut(s) 1043, 2244, 2497
TfiI GAWTC 5 cut(s) 1094, 1130, 1673, 2082, 2560
TscAI CASTG 4 cut(s) 205, 290, 1064, 1594
TseFI GTSAC 5 cut(s) 86, 766, 1678, 1738, 1826
TseI GCWGC 7 cut(s) 152, 248, 425, 684, 1607, 2434, 2491
Tsp45I GTSAC 5 cut(s) 86, 766, 1678, 1738, 1826
TspDTI ATGAA 9 cut(s) 17, 906, 1056, 1437, 1679, 2003, 2573, 2581, 2609
TspGWI ACGGA 1 cut(s) 1986
TspRI CASTG 4 cut(s) 205, 290, 1064, 1594
Van91I CCANNNNNTGG 1 cut(s) 1175
Vha464I CTTAAG 2 cut(s) 1031, 1199
VpaK11BI GGWCC 1 cut(s) 790
XapI RAATTY 5 cut(s) 436, 1020, 1424, 1613, 2596
XbaI TCTAGA 1 cut(s) 431
XceI RCATGY 5 cut(s) 417, 967, 1040, 2100, 2309
XcmI CCANNNNNNNNNTGG 1 cut(s) 1342
XmiI GTMKAC 1 cut(s) 1893
XmnI GAANNNNTTC 2 cut(s) 1861, 1964
XspI CTAG 7 cut(s) 123, 432, 570, 794, 846, 1361, 2498
ZrmI AGTACT 1 cut(s) 121
Zsp2I ATGCAT 3 cut(s) 965, 1311, 2174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.