Rh1CG184300

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000280
Physical Location & Seq
Reverse (-)
40440859 .. 40450347
9489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG184300.1

Sequence Viewer

Length: 3207 bp
ATGTGCCATCAGTGTCAGAGGAATGATAGGGCCAGAGTTGTTAACTGCACAAGTTGTAAGCGGAAGCGATTTTGTGTATCATGCATTCAGAATTGGTATCCCCAGATGTCAGAGGAGGCAATTGCTGATGCTTGTCCTGTATGCCTTGGAAATTGCAATTGCAAAGCGTGCTTGCGTTTAGATGAAACTAATCATAATTTGAATAGCAAGCTTGCATTGCACGTTAGCAAAGATGAAGAAGTGGAGCACTCTAAGTATTTGCTTGAAGCATTGCTTCCGTTCTTGAAAAGACTTAATGATGAACAAGTTATAGAGATGGTGATGGAGGCTAGGAGACAAGGGAAATCTCTTTCGGAGCTAAAGATCCAAAGGTCGGACAGCTCTCCTGATGATCGTGTATACTGCGACAACTGCAATACATCTATTATTGATTTCCACAGAAGCTGTCCTATATGTTCATATGACCTTTGTCTTATCTGCTGCCGGGAGATTCGAGATGGAAACCAGCAAGGAAGTGCAGAAGAAGTGATTGGGGAGTATATCAATCAAGGTCTAGACTATTTACATGGTGGAAAGGGCAACTTAATAAAAAAGCCTTCTGAAACTAAACTCAAGTGTCCTGTAAGGTCAACATCTAAGTGGAAAGTAAGCAAAGATCGAAGTATTTTTTGCCCCTCAAAAGACATGGACGGCTGTGGTTGTAGTGTTCTAGAACTGAGATGCATATTTTCTGAAAACCACATCATAGAGTTGGTAGAAAAAGCAGAAGAAATTGCAGAAACTAAAAAACTGATGTATGCAGCTGGACCTTCTGCACAGAAGTTTTCATGGTTCAACTCTGCAGGTGGTGCTGACTCAAGAGGCAATGAATTGACGAAAGCAGCATCTCGAGAGGATTCGGAGGACAACTACTTGTACAGTCCAAGGGCTATAAATATCCAACATGAAGACTTTAAGCATTTTCGCTGTCATTGGATCCAGGGTGAGCCTGTGATTGTGGGGAATGTGCTTGAAACTGCAACAGGTTTGAGCTGGGAACCATTTGTCATGTGGCGTGCTTGTCGTCAGATGAGAAATACCGGGCATGATAGACATGTGGAATTTAAGGCCATTGATTGCTTGGATTGGTGTGAGCTAGATATCGGTATCCACCAATTTTTTACTGGATATTTGGAGGGCCAGTTTGATGGGAAAATGTGGCCTCGTATTCTGAAACTCAACGATCAGTCCCTTGATGATTATTTGGAGAAGCGCCTTCCTCGTCATTGTGCTGAGTTTATTTGTTGCTTGCCATTTAAGGAATATACCCATCCTCGCAGTGGCCTCCTGAACCTTGCTGTAAAATTGCCTATGGAGTGTGTAAAGCCAGACATGGGGCCAAAAATGGATATTGCTTATGGTGTGTCCCAGGAGCTTGGACGTGGAGATTCAGTAACTAAGCTTCACTGTGATTCATGTGATGTGGTGAATGTTTTGACACATACTGCTGAAGTGACGATTACTGCTGAGCAACTTTGTACTATAGAAGGGTTGAAAAAAGAGCACATTGAACAAGACCGAAGGGAAATGTTTGAAAATTATGAGACTGTGGATGACAATGTTGATCATAAGGAGTCTGGCAGTGGGTCTTGTGTCAGGACTGCAAAGGGTAAACAATGTTGTACTCAAGTGAAGTATCAAAACAGGGTTGCAATTTTTCAAGAAAAGACTAAAGCTCAAACTGATAGTTGGAATGGTGGAATTTTTTCAAAGGAATCCATGTCTGAAAAGAATGCAGAGGGAGGTGCTTTATGGGACATTTTTAGACGACAGGATGTTCCTAAGCTGCAGGAATATCTATGGAAACACTACAAGGAATTCAGGCACACCTACTGTTGTCCATTGCAGCAGGTCACTCACCCCATACATGATCAGACTTTTTATCTGACATTAGAGCACAAGAGGAAGCTGAAGGAAGAATATGGAATTGAACCATGGACATTTATCCAGAAACTTGGAGATGCTGTCTTTATTCCTGCAGGATGTCCACACCAAGTTAGAAACTTGAAGTCGTGCATTAAAGTTTCTGTTGAATTTGTCTCCCCTGAAAATGTTGGTGAATGCTTACGTCTGACAGATGAGTTCCGTACACTTCCACGAAAGCATATAGCTAAAGAGGACAAATTGGAGGTGAAGAAAATGATTGTTCATGCTGTACATGAGGCTGTGGAGATTTTGGATCAGAATTTGAGGTTATGTAGCGTTAAAAACTTCAGGGGAGAGAAGCCTTCACAGCCCCCTGTTAATATATGTAAGGGCAACATTGCAAACAAATTGAGAGAGAAAGCAGCTAGAGGACGACCACAAGAAGAATCAAAAGAGGTTCAAATTCTTGAGGAGCAGCCTTCACAATTATCACAGCCTATAAAGCCTGCTATTGATGTTGGTAGATTTGGACGCACACGGAGAGAGGAAAAAGATGGAGGGCAACCACAAGAAGCAAGTAGAGTAGATGAAGAGGTCCAAATTCTTAAGGGACAGCCTTCACAGTCAGCACAACATGTACAGCCCACTGCTTATGTTGATAGCAAGCATATGATGAGAAAGGAAACAGAGGGAGGGGTACTGCAAGAAACACATGGAGAAGTTGAAGACGTCCAAATTCTTGAGAAGCAGCCTTGGCAATCATCACAAACTGTACAAGCCACTCTTCATGTTGGTAGCATTGAACATGCATCTAGAGAGGAAAAAGCTGGATGGCAACCACAAAAAACAAGTGGAGAAGATGAAGAAGTCCAAATTCTTGAGGAGCAGCATTGGCAATCATCAAAATCTGTACAAGCAACTGTTCACATTGGTAGCATTGGACTTGCAATGAGAGAGGAAAAAGTGGGAGGACGACAGCATGAAGGAAGGGGAGAACAGGAAGAGGTCCGAAATCTTGGTGAAAAGCCTTCAAAGTTATCACAACCTGAACCTGTCCAGCCCACTGTTGATGTTGATAAAGCGGTGCAGCAACTGGATGAGCTCGTGGAGCTCCTTAGACATTTTGAACAGAGGAGGATACCGATGGGAGGGAGAGATGGAAGAATCAGTTCTCAATTCCAGCTTTTATGCTCGGAGTTCTTGACTTTATCTTTGCATCTTGGTTATGCACCTAATGATACCAAACCTCACACTACACCATCTGCTCAGCCGCTACATGATCCTCCAGTTGTAGATCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1068

Amino Acids

121.67

Weight (kDa)

6.06

Isoelectric Point (pI)

55.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 2 - 62 2.7e-06 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 589 - 692 2.3e-14 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 833
AatII GACGTC 1 cut(s) 2637
Acc36I ACCTGC 2 cut(s) 833, 1879
AccI GTMKAC 1 cut(s) 399
AciI CCGC 3 cut(s) 61, 2987, 3176
AclWI GGATC 5 cut(s) 358, 970, 983, 2226, 3179
AcsI RAATTY 9 cut(s) 1100, 1740, 1856, 2072, 2224, 2367, 2505, 2640, 2778
AcuI CTGAAG 3 cut(s) 1509, 1970, 2236
AcyI GRCGYC 1 cut(s) 2634
AfaI GTAC 9 cut(s) 917, 1519, 1663, 2128, 2196, 2544, 2604, 2679, 2817
AfiI CCNNNNNNNGG 4 cut(s) 373, 1319, 1373, 3053
AflII CTTAAG 1 cut(s) 2510
AflIII ACRYGT 2 cut(s) 1093, 2539
AjiI CACGTC 1 cut(s) 1421
AjnI CCWGG 2 cut(s) 978, 1407
AjuI GAANNNNNNNTTGG 2 cut(s) 513, 545
Alw21I GWGCWC 5 cut(s) 249, 1545, 1938, 3009, 3018
Alw26I GTCTC 3 cut(s) 328, 1577, 2083
AlwI GGATC 5 cut(s) 358, 970, 983, 2226, 3179
AlwNI CAGNNNCTG 2 cut(s) 444, 2998
Ama87I CYCGRG 1 cut(s) 888
AoxI GGCC 6 cut(s) 30, 1107, 1177, 1199, 1321, 1376
ApoI RAATTY 9 cut(s) 1100, 1740, 1856, 2072, 2224, 2367, 2505, 2640, 2778
ArsI GACNNNNNNTTYG 2 cut(s) 3100, 3132
Asp700I GAANNNNTTC 2 cut(s) 1744, 3073
AspLEI GCGC 1 cut(s) 1254
AspS9I GGNCC 6 cut(s) 30, 806, 1177, 1376, 2500, 2911
AsuC2I CCSGG 2 cut(s) 485, 1081
AsuHPI GGTGA 7 cut(s) 331, 995, 1477, 1889, 2108, 2182, 2936
AvaI CYCGRG 1 cut(s) 888
AvaII GGWCC 3 cut(s) 806, 2500, 2911
BamHI GGATCC 1 cut(s) 975
BanII GRGCYC 2 cut(s) 3009, 3018
BarI GAAGNNNNNNTAC 2 cut(s) 1425, 1457
BauI CACGAG 1 cut(s) 3008
BbsI GAAGAC 2 cut(s) 954, 2637
Bbv12I GWGCWC 5 cut(s) 249, 1545, 1938, 3009, 3018
BceAI ACGGC 1 cut(s) 706
BciT130I CCWGG 2 cut(s) 980, 1409
BciVI GTATCC 3 cut(s) 108, 1157, 3036
BclI TGATCA 2 cut(s) 1603, 1909
BcnI CCSGG 2 cut(s) 485, 1081
BcoDI GTCTC 3 cut(s) 328, 1577, 2083
BfaI CTAG 6 cut(s) 330, 554, 710, 1136, 2331, 2718
BfmI CTRYAG 4 cut(s) 840, 1521, 1826, 2016
BfoI RGCGCY 1 cut(s) 1255
BfrI CTTAAG 1 cut(s) 2510
BfuAI ACCTGC 2 cut(s) 833, 1879
BfuI GTATCC 3 cut(s) 108, 1157, 3036
BglII AGATCT 1 cut(s) 3199
BlpI GCTNAGC 2 cut(s) 1506, 3171
Bme1390I CCNGG 4 cut(s) 485, 980, 1081, 1409
Bme18I GGWCC 3 cut(s) 806, 2500, 2911
BmeT110I CYCGRG 1 cut(s) 888
BmgBI CACGTC 1 cut(s) 1421
BmgT120I GGNCC 6 cut(s) 30, 806, 1177, 1376, 2500, 2911
BmiI GGNNCC 3 cut(s) 977, 1038, 1377
BmrFI CCNGG 4 cut(s) 485, 980, 1081, 1409
BmsI GCATC 6 cut(s) 118, 710, 893, 1990, 2723, 3130
BoxI GACNNNNGTC 1 cut(s) 468
BpiI GAAGAC 2 cut(s) 954, 2637
BpmI CTGGAG 1 cut(s) 3174
Bpu10I CCTNAGC 1 cut(s) 1821
Bpu1102I GCTNAGC 2 cut(s) 1506, 3171
BpuEI CTTGAG 6 cut(s) 596, 841, 1650, 2393, 2666, 2804
BpuMI CCSGG 2 cut(s) 485, 1081
BsaHI GRCGYC 1 cut(s) 2634
BsaJI CCNNGG 6 cut(s) 145, 923, 979, 1407, 1973, 2657
BsaXI ACNNNNNCTCC 6 cut(s) 1166, 1196, 1416, 1446, 1989, 2019
Bsc4I CCNNNNNNNGG 4 cut(s) 373, 1319, 1373, 3053
Bse1I ACTGG 4 cut(s) 1168, 1180, 3003, 3191
Bse3DI GCAATG 6 cut(s) 215, 269, 871, 1880, 2301, 2859
BseBI CCWGG 2 cut(s) 980, 1409
BseDI CCNNGG 6 cut(s) 145, 923, 979, 1407, 1973, 2657
BseGI GGATG 6 cut(s) 1309, 1597, 1819, 2027, 2741, 3007
BseLI CCNNNNNNNGG 4 cut(s) 373, 1319, 1373, 3053
BseMI GCAATG 6 cut(s) 215, 269, 871, 1880, 2301, 2859
BseMII CTCAG 4 cut(s) 707, 1263, 1497, 3185
BseNI ACTGG 4 cut(s) 1168, 1180, 3003, 3191
BseRI GAGGAG 4 cut(s) 128, 2390, 2801, 3052
BseYI CCCAGC 1 cut(s) 1032
BsgI GTGCAG 4 cut(s) 31, 537, 798, 3011
BshFI GGCC 6 cut(s) 32, 1109, 1179, 1201, 1323, 1378
BsiHKAI GWGCWC 5 cut(s) 249, 1545, 1938, 3009, 3018
BsiHKCI CYCGRG 1 cut(s) 888
BsiSI CCGG 2 cut(s) 484, 1080
BslFI GGGAC 4 cut(s) 1213, 1390, 1808, 2529
BslI CCNNNNNNNGG 4 cut(s) 373, 1319, 1373, 3053
BsmAI GTCTC 3 cut(s) 328, 1577, 2083
BsmFI GGGAC 4 cut(s) 1213, 1390, 1808, 2529
BsmI GAATGC 3 cut(s) 84, 1777, 2105
BsnI GGCC 6 cut(s) 32, 1109, 1179, 1201, 1323, 1378
BsoBI CYCGRG 1 cut(s) 888
Bsp1286I GDGCHC 5 cut(s) 249, 1545, 1938, 3009, 3018
Bsp1407I TGTACA 5 cut(s) 915, 2194, 2542, 2677, 2815
Bsp1720I GCTNAGC 2 cut(s) 1506, 3171
Bsp19I CCATGG 1 cut(s) 1973
BspACI CCGC 3 cut(s) 61, 2987, 3176
BspANI GGCC 6 cut(s) 32, 1109, 1179, 1201, 1323, 1378
BspCNI CTCAG 4 cut(s) 708, 1264, 1498, 3184
BspLI GGNNCC 3 cut(s) 977, 1038, 1377
BspMAI CTGCAG 3 cut(s) 844, 1830, 2020
BspMI ACCTGC 2 cut(s) 833, 1879
BspPI GGATC 5 cut(s) 358, 970, 983, 2226, 3179
BspTI CTTAAG 1 cut(s) 2510
BsrDI GCAATG 6 cut(s) 215, 269, 871, 1880, 2301, 2859
BsrGI TGTACA 5 cut(s) 915, 2194, 2542, 2677, 2815
BsrI ACTGG 4 cut(s) 1168, 1180, 3003, 3191
BssECI CCNNGG 6 cut(s) 145, 923, 979, 1407, 1973, 2657
BssNAI GTATAC 1 cut(s) 400
BssNI GRCGYC 1 cut(s) 2634
BssSI CACGAG 1 cut(s) 3008
BssT1I CCWWGG 4 cut(s) 145, 923, 1973, 2657
Bst1107I GTATAC 1 cut(s) 400
Bst2BI CACGAG 1 cut(s) 3008
Bst2UI CCWGG 2 cut(s) 980, 1409
Bst4CI ACNGT 8 cut(s) 920, 1448, 1588, 1874, 2529, 2677, 2827, 2971
Bst6I CTCTTC 3 cut(s) 2490, 2694, 2901
BstACI GRCGYC 1 cut(s) 2634
BstAFI CTTAAG 1 cut(s) 2510
BstAPI GCANNNNNTGC 2 cut(s) 168, 848
BstAUI TGTACA 5 cut(s) 915, 2194, 2542, 2677, 2815
BstC8I GCNNGC 8 cut(s) 169, 173, 209, 213, 1056, 1289, 2412, 2570
BstDEI CTNAG 9 cut(s) 252, 636, 716, 1272, 1437, 1506, 1821, 3020, 3171
BstDSI CCRYGG 1 cut(s) 1973
BstF5I GGATG 6 cut(s) 1309, 1597, 1819, 2027, 2741, 3007
BstH2I RGCGCY 1 cut(s) 1255
BstHHI GCGC 1 cut(s) 1254
BstMAI GTCTC 3 cut(s) 328, 1577, 2083
BstMWI GCNNNNNNNGC 9 cut(s) 168, 217, 411, 848, 2272, 2407, 2659, 2797, 3013
BstNI CCWGG 2 cut(s) 980, 1409
BstNSI RCATGY 3 cut(s) 1097, 2543, 2714
BstPAI GACNNNNGTC 1 cut(s) 468
BstSCI CCNGG 4 cut(s) 483, 978, 1079, 1407
BstSFI CTRYAG 4 cut(s) 840, 1521, 1826, 2016
BstV2I GAAGAC 2 cut(s) 954, 2637
BstX2I RGATCY 3 cut(s) 363, 975, 3199
BstXI CCANNNNNNTGG 3 cut(s) 1187, 1415, 1994
BstYI RGATCY 3 cut(s) 363, 975, 3199
BstZ17I GTATAC 1 cut(s) 400
BsuI GTATCC 3 cut(s) 108, 1157, 3036
BsuRI GGCC 6 cut(s) 32, 1109, 1179, 1201, 1323, 1378
BtgI CCRYGG 1 cut(s) 1973
BtrI CACGTC 1 cut(s) 1421
BtsCI GGATG 6 cut(s) 1309, 1597, 1819, 2027, 2741, 3007
BtsI GCAGTG 3 cut(s) 1324, 1627, 2550
BtsIMutI CAGTG 6 cut(s) 17, 1324, 1444, 1627, 2550, 2967
BveI ACCTGC 2 cut(s) 833, 1879
Cac8I GCNNGC 8 cut(s) 169, 173, 209, 213, 1056, 1289, 2412, 2570
CaiI CAGNNNCTG 2 cut(s) 444, 2998
CfoI GCGC 1 cut(s) 1254
Cfr13I GGNCC 6 cut(s) 30, 806, 1177, 1376, 2500, 2911
CseI GACGC 1 cut(s) 2445
Csp6I GTAC 9 cut(s) 916, 1518, 1662, 2127, 2195, 2543, 2603, 2678, 2816
CviQI GTAC 9 cut(s) 916, 1518, 1662, 2127, 2195, 2543, 2603, 2678, 2816
DdeI CTNAG 9 cut(s) 252, 636, 716, 1272, 1437, 1506, 1821, 3020, 3171
Eam1104I CTCTTC 3 cut(s) 2490, 2694, 2901
EarI CTCTTC 3 cut(s) 2490, 2694, 2901
Ecl136II GAGCTC 2 cut(s) 3007, 3016
Eco130I CCWWGG 4 cut(s) 145, 923, 1973, 2657
Eco24I GRGCYC 2 cut(s) 3009, 3018
Eco32I GATATC 1 cut(s) 1141
Eco47I GGWCC 3 cut(s) 806, 2500, 2911
Eco53kI GAGCTC 2 cut(s) 3007, 3016
Eco57I CTGAAG 3 cut(s) 1509, 1970, 2236
Eco88I CYCGRG 1 cut(s) 888
EcoICRI GAGCTC 2 cut(s) 3007, 3016
EcoRI GAATTC 1 cut(s) 1856
EcoRII CCWGG 2 cut(s) 978, 1407
EcoRV GATATC 1 cut(s) 1141
EcoT14I CCWWGG 4 cut(s) 145, 923, 1973, 2657
EcoT22I ATGCAT 3 cut(s) 86, 725, 2716
EcoT38I GRGCYC 2 cut(s) 3009, 3018
ErhI CCWWGG 4 cut(s) 145, 923, 1973, 2657
FalI AAGNNNNNCTT 6 cut(s) 258, 290, 566, 598, 2673, 2705
FaqI GGGAC 4 cut(s) 1213, 1390, 1808, 2529
FauNDI CATATG 2 cut(s) 460, 2574
FbaI TGATCA 2 cut(s) 1603, 1909
FblI GTMKAC 1 cut(s) 399
FokI GGATG 6 cut(s) 1296, 1604, 1826, 2034, 2748, 3014
FriOI GRGCYC 2 cut(s) 3009, 3018
FspBI CTAG 6 cut(s) 330, 554, 710, 1136, 2331, 2718
GlaI GCGC 1 cut(s) 1253
GsaI CCCAGC 1 cut(s) 1036
GsuI CTGGAG 1 cut(s) 3174
HaeII RGCGCY 1 cut(s) 1255
HaeIII GGCC 6 cut(s) 32, 1109, 1179, 1201, 1323, 1378
HapII CCGG 2 cut(s) 484, 1080
HgaI GACGC 1 cut(s) 2445
HhaI GCGC 1 cut(s) 1254
Hin1I GRCGYC 1 cut(s) 2634
Hin6I GCGC 1 cut(s) 1252
HinP1I GCGC 1 cut(s) 1252
HincII GTYRAC 2 cut(s) 43, 630
HindII GTYRAC 2 cut(s) 43, 630
HindIII AAGCTT 2 cut(s) 209, 1439
HinfI GANTC 9 cut(s) 490, 854, 896, 1427, 1451, 1613, 1754, 2351, 3069
HpaI GTTAAC 1 cut(s) 43
HpaII CCGG 2 cut(s) 484, 1080
HphI GGTGA 7 cut(s) 331, 995, 1477, 1889, 2108, 2182, 2936
Hpy166II GTNNAC 7 cut(s) 43, 400, 630, 1652, 2027, 2129, 2830
Hpy8I GTNNAC 7 cut(s) 43, 400, 630, 1652, 2027, 2129, 2830
HpyCH4III ACNGT 8 cut(s) 920, 1448, 1588, 1874, 2529, 2677, 2827, 2971
HpyCH4IV ACGT 4 cut(s) 222, 1420, 2107, 2634
HpyF10VI GCNNNNNNNGC 9 cut(s) 168, 217, 411, 848, 2272, 2407, 2659, 2797, 3013
HpyF3I CTNAG 9 cut(s) 252, 636, 716, 1272, 1437, 1506, 1821, 3020, 3171
HpySE526I ACGT 4 cut(s) 222, 1420, 2107, 2634
Hsp92I GRCGYC 1 cut(s) 2634
HspAI GCGC 1 cut(s) 1252
Ksp22I TGATCA 2 cut(s) 1603, 1909
KspAI GTTAAC 1 cut(s) 43
LmnI GCTCC 7 cut(s) 244, 355, 1411, 2377, 2788, 3013, 3021
LweI GCATC 6 cut(s) 118, 710, 893, 1990, 2723, 3130
MaeI CTAG 6 cut(s) 330, 554, 710, 1136, 2331, 2718
MaeII ACGT 4 cut(s) 222, 1420, 2107, 2634
MaeIII GTNAC 3 cut(s) 1432, 1492, 1891
MfeI CAATTG 2 cut(s) 120, 157
MflI RGATCY 3 cut(s) 363, 975, 3199
MhlI GDGCHC 5 cut(s) 249, 1545, 1938, 3009, 3018
MlyI GAGTC 2 cut(s) 848, 1622
MmeI TCCRAC 3 cut(s) 354, 964, 1709
Mph1103I ATGCAT 3 cut(s) 86, 725, 2716
MroXI GAANNNNTTC 2 cut(s) 1744, 3073
MslI CAYNNNNRTG 1 cut(s) 637
MspA1I CMGCKG 1 cut(s) 803
MspCI CTTAAG 1 cut(s) 2510
MspI CCGG 2 cut(s) 484, 1080
MspR9I CCNGG 4 cut(s) 485, 980, 1081, 1409
MunI CAATTG 2 cut(s) 120, 157
Mva1269I GAATGC 3 cut(s) 84, 1777, 2105
MvaI CCWGG 2 cut(s) 980, 1409
MwoI GCNNNNNNNGC 9 cut(s) 168, 217, 411, 848, 2272, 2407, 2659, 2797, 3013
NciI CCSGG 2 cut(s) 485, 1081
NcoI CCATGG 1 cut(s) 1973
NdeI CATATG 2 cut(s) 460, 2574
NlaIV GGNNCC 3 cut(s) 977, 1038, 1377
NmuCI GTSAC 2 cut(s) 1492, 1891
NsiI ATGCAT 3 cut(s) 86, 725, 2716
NspI RCATGY 3 cut(s) 1097, 2543, 2714
PaeR7I CTCGAG 1 cut(s) 888
PaqCI CACCTGC 1 cut(s) 833
PciI ACATGT 2 cut(s) 1093, 2539
PctI GAATGC 3 cut(s) 84, 1777, 2105
PdmI GAANNNNTTC 2 cut(s) 1744, 3073
PfeI GAWTC 7 cut(s) 490, 896, 1427, 1451, 1754, 2351, 3069
PleI GAGTC 2 cut(s) 848, 1621
PpsI GAGTC 2 cut(s) 848, 1621
PscI ACATGT 2 cut(s) 1093, 2539
PshAI GACNNNNGTC 1 cut(s) 468
Psp124BI GAGCTC 2 cut(s) 3009, 3018
Psp6I CCWGG 2 cut(s) 978, 1407
PspFI CCCAGC 1 cut(s) 1032
PspGI CCWGG 2 cut(s) 978, 1407
PspN4I GGNNCC 3 cut(s) 977, 1038, 1377
PspPI GGNCC 6 cut(s) 30, 806, 1177, 1376, 2500, 2911
PstI CTGCAG 3 cut(s) 844, 1830, 2020
PstNI CAGNNNCTG 2 cut(s) 444, 2998
PsuI RGATCY 3 cut(s) 363, 975, 3199
PvuII CAGCTG 1 cut(s) 803
RsaI GTAC 9 cut(s) 917, 1519, 1663, 2128, 2196, 2544, 2604, 2679, 2817
RsaNI GTAC 9 cut(s) 916, 1518, 1662, 2127, 2195, 2543, 2603, 2678, 2816
RseI CAYNNNNRTG 1 cut(s) 637
SacI GAGCTC 2 cut(s) 3009, 3018
Sau96I GGNCC 6 cut(s) 30, 806, 1177, 1376, 2500, 2911
SbfI CCTGCAGG 1 cut(s) 2020
SchI GAGTC 2 cut(s) 848, 1622
ScrFI CCNGG 4 cut(s) 485, 980, 1081, 1409
SdaI CCTGCAGG 1 cut(s) 2020
SduI GDGCHC 5 cut(s) 249, 1545, 1938, 3009, 3018
SfaNI GCATC 6 cut(s) 118, 710, 893, 1990, 2723, 3130
SfcI CTRYAG 4 cut(s) 840, 1521, 1826, 2016
Sfr274I CTCGAG 1 cut(s) 888
SinI GGWCC 3 cut(s) 806, 2500, 2911
SlaI CTCGAG 1 cut(s) 888
SmiMI CAYNNNNRTG 1 cut(s) 637
SmlI CTYRAG 8 cut(s) 611, 856, 888, 1665, 2372, 2510, 2645, 2783
SmoI CTYRAG 8 cut(s) 611, 856, 888, 1665, 2372, 2510, 2645, 2783
Sse8387I CCTGCAGG 1 cut(s) 2020
SsiI CCGC 3 cut(s) 61, 2987, 3176
SspMI CTAG 6 cut(s) 330, 554, 710, 1136, 2331, 2718
SstI GAGCTC 2 cut(s) 3009, 3018
StyD4I CCNGG 4 cut(s) 483, 978, 1079, 1407
StyI CCWWGG 4 cut(s) 145, 923, 1973, 2657
TaaI ACNGT 8 cut(s) 920, 1448, 1588, 1874, 2529, 2677, 2827, 2971
TaiI ACGT 4 cut(s) 225, 1423, 2110, 2637
TaqI TCGA 3 cut(s) 493, 658, 889
TaqII GACCGA 1 cut(s) 1572
TatI WGTACW 7 cut(s) 915, 1517, 1661, 2194, 2542, 2677, 2815
TauI GCSGC 1 cut(s) 3178
TfiI GAWTC 7 cut(s) 490, 896, 1427, 1451, 1754, 2351, 3069
TscAI CASTG 6 cut(s) 17, 1324, 1451, 1627, 2557, 2974
TseFI GTSAC 2 cut(s) 1492, 1891
Tsp45I GTSAC 2 cut(s) 1492, 1891
TspGWI ACGGA 3 cut(s) 267, 2114, 2458
TspRI CASTG 6 cut(s) 17, 1324, 1451, 1627, 2557, 2974
Vha464I CTTAAG 1 cut(s) 2510
VpaK11BI GGWCC 3 cut(s) 806, 2500, 2911
XapI RAATTY 9 cut(s) 1100, 1740, 1856, 2072, 2224, 2367, 2505, 2640, 2778
XbaI TCTAGA 3 cut(s) 553, 709, 2717
XceI RCATGY 3 cut(s) 1097, 2543, 2714
XcmI CCANNNNNNNNNTGG 4 cut(s) 1047, 1117, 1160, 1316
XhoI CTCGAG 1 cut(s) 888
XmiI GTMKAC 1 cut(s) 399
XmnI GAANNNNTTC 2 cut(s) 1744, 3073
XspI CTAG 6 cut(s) 330, 554, 710, 1136, 2331, 2718
ZraI GACGTC 1 cut(s) 2635
Zsp2I ATGCAT 3 cut(s) 86, 725, 2716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.