Rh7BG355000

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000280
Physical Location & Seq
Forward (+)
37204160 .. 37207569
3410 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG355000.1

Sequence Viewer

Length: 2898 bp
ATGGCGGCGAGAAAGAAAAGCAGAGGCAGAGGCCGGCCGAGGCAAGAAGAGAACGGCGCTGGTCTGGAGGAGATGAAGGCCGAGGGTATTTTGGGATTTCAGAACGAGGGAGCTAATGGGGAAGTAAAAGAAGAAGCTGTGGAGCCGAAGCCAGAGTCGGCTCCTTTGGTGGAGGGCAGAGGCAGGAAGCGGCGGAGGAAGAAACAAGAAGAAGGCGGTGGTGGTGAGGGTATTTTGGGAAATCAAAACGAGGAAGAGGTTGCTGAATATGGTGTGGAGGTAAAGAGGCGTTGTCCACAGAGGGCGGCAAGGGCTGTAAAGGCGGTGGTGGAGGAGGAGGAGGAGGAGGAGCCGGAGATTCGGAAGATGAAGAAGAGAAAAGCAGCGGCGGCGGAGGGGGAGGAAGATGAGGCAATGAAAGACGTTTCAACAATGTGCCATCAGTGTCAGAGGAATGATAAAGGGAGAGTGGTTCGGTGCGCGAGTTGTCCGAAAAGGGGTAAGGGAATGAGGAAGAGGTATTGTATCCCTTGTATACAGAGGTGGTATCCCAATACATCAGAGGAAGCCATTGCTGAGGCTTGTCCTGTGTGCCGCGGCAACTGCAACTGCAAAGCCTGCCTTCGATTGGATGTGCCTAATAGGTGTTTGAAGAATCTAGACCCGAAAATCGACGAGGAGACCAAGCTGGAGCACTGTAAGTATTTGGTTCATCAGCTGCTTCCGTATTTGAAGAGGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTCGAAGCCAAGAGGCAGGGATTAAGTGATGTTTCGGAGCTCAAGGTGGAGAAGGCTAATTGTGCTGACGATGAGCGTATGTACTGCAATAACTGCAGCACTTCGATTTTTGATTTTCATAGGAGTTGTCCTGAGTGTGAGTATGATCTGTGCCTCATTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAAGAAGAGAGGTATGGAGGAGGAAGAGAGTCAGAAGTATGTCGATTTGGGGCGTGAGTATTTGCATGGAAAGGGAGAGTTGAAGAGCTTGCTAAAAAAAAAGAGAGAAAAGGAGTTGAAGAGCTTGCTAAAGAAGAAAAGAGAAGAAGAGTTGAAGGAGAATGTTGATGTGGGAGTGAAGCTGGACTCGTCAGAATCTGCTCAAGAGTCCACGATAGCAAGTGAAGATGGAAATATTCAAGATGGGAAGCAGAAAAAGAGAGGCAGAGAGGATGAAGTAAGTGAGGAATTTGTTAATGCGGGGGAGTGTTTGGATGGAGAGGGAGAGCTGAAGAGGAATGATGATGTGGGAGTGAAGCAGGACGAGTCCGAATCTGCTGAAGAGTCCACATTTGAGTGGAGAGCAAGTGAAGATGGAAGCATTCTCTGCCCCCCAACACACATGAAAGGTTGTGGTAAGCATAATTTAGAATTGAGGTGCAATTTTTCTCAGAATCATGTCATAGACTTGGTAGAGAAAGCAGGAAAAATAGATGAAAGTTACAATCCTATGCATGCTAGTAGTGAAACATGCACACAAAAGTGTTCATGTGTTAAACCTGTGAATGATGCTGTCAACTCAAGTAGCAGCAGCTGTAAGTTAAGAAAAGCAGCTTCTAGAGACGGTTCTGATGATAACTACTTGTTCTGTCCAAGGGTCGGAGAAATCCAAGATGAGGATTTTGGGCATTTTCAGTGGCATTGGATGAGGGGTGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCAGGTTTGAGCTGGGAACCATTAGTCATGTGGCGTGCATTTAGGCAGATAAAGAATCTCAAGCATGATAGAGAATTGGCAGTCCATACCATTGAATGCTTGGATTGGAGTTTCAAAGAAACTAATAACCTCCATTTCTTTACTGGATATTCCGAAGGCCGGCCTGATGAGAAAGGATGGCCTGAGATTCTCAAGCTCAAAGACTTCCCGCCATCTACTGAGTTTGACAATCGTCTCCCCCGGCATGGTAAGGAATTTGTCTGTTGTCTGCCTTTCAAAGAGTATACTCACCCCAACAAGGGAACCCTGAACCTTGCCATCCACTTGCCACCTACCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACGGGGAGATTCTGTGACCAAGCTTCACTGCGATATGTCTGATGCGGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACAACTGAACAACTTGCAACTATAGATAAGTTGAAAAAAAAGCACAGGAAGCAAGACGAAAGAGAGATATTTGGGAATTGTCACACGAGTGCTGGAAATGGTAGTGGGCCTCAGGAAGAATCAGCTGAAGTATGTGAGGATGGTGGTGCCTTATGGGACATTTTCCGGATCCAGGATGTTCCGCAGCTAGAGAAATACATCGACAAGCACATCAATGAATTTAGGCATGTGCACTGTAATCCTTTGCAGCGTGTTACTCATGCTATACATGATCAGACTGTTTATCTCACTGTGGAGCACAAGAGGAAGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTGGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGCCAAACCACAGGGCCAGCGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTGTGAAGGAGATTTCTGGTGAGAATGCGAAGCCTGAGGATGTGAAGCCTAAGAGGAGGTGCAAGGGGCCTAAGAGGAGGCGCAAGGGGAACAAAGGAGAGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

965

Amino Acids

109.66

Weight (kDa)

6.37

Isoelectric Point (pI)

53.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 141 - 209 1.2e-06 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 809 - 888 1e-12 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 742
Acc36I ACCTGC 2 cut(s) 742, 2597
AccB1I GGYRCC 1 cut(s) 2372
AccI GTMKAC 2 cut(s) 535, 2030
AccII CGCG 2 cut(s) 482, 597
AccIII TCCGGA 1 cut(s) 2391
AclWI GGATC 3 cut(s) 1753, 2389, 2402
AcoI YGGCCR 1 cut(s) 35
AcsI RAATTY 4 cut(s) 1244, 2000, 2444, 2546
AcuI CTGAAG 4 cut(s) 1307, 1356, 2235, 2373
AfaI GTAC 1 cut(s) 851
AjnI CCWGG 1 cut(s) 2397
AleI CACNNNNGTG 2 cut(s) 1537, 2313
Alw21I GWGCWC 4 cut(s) 696, 810, 2460, 2526
Alw26I GTCTC 3 cut(s) 674, 1611, 1985
Alw44I GTGCAC 1 cut(s) 2456
AlwI GGATC 3 cut(s) 1753, 2389, 2402
AlwNI CAGNNNCTG 2 cut(s) 1154, 1590
Aor13HI TCCGGA 1 cut(s) 2391
ApaLI GTGCAC 1 cut(s) 2456
ApeKI GCWGC 9 cut(s) 383, 718, 864, 930, 1584, 1587, 1607, 2410, 2473
ApoI RAATTY 4 cut(s) 1244, 2000, 2444, 2546
ArsI GACNNNNNNTTYG 2 cut(s) 140, 172
Asp700I GAANNNNTTC 1 cut(s) 2709
AspLEI GCGC 3 cut(s) 59, 482, 2874
AspS9I GGNCC 5 cut(s) 2102, 2221, 2333, 2736, 2858
AsuC2I CCSGG 2 cut(s) 935, 1987
AsuHPI GGTGA 8 cut(s) 236, 766, 1721, 2027, 2635, 2696, 2770, 2822
AvaII GGWCC 1 cut(s) 2102
AxyI CCTNAGG 2 cut(s) 2337, 2826
BaeGI GKGCMC 1 cut(s) 2460
BamHI GGATCC 1 cut(s) 2394
BanI GGYRCC 1 cut(s) 2372
BanII GRGCYC 1 cut(s) 810
BauI CACGAG 1 cut(s) 2311
Bbv12I GWGCWC 4 cut(s) 696, 810, 2460, 2526
BbvCI CCTCAGC 1 cut(s) 576
BbvI GCAGC 9 cut(s) 395, 705, 876, 917, 1596, 1599, 1619, 2422, 2485
BceAI ACGGC 2 cut(s) 70, 2545
BciT130I CCWGG 1 cut(s) 2399
BciVI GTATCC 2 cut(s) 536, 558
BclI TGATCA 1 cut(s) 2497
BcnI CCSGG 2 cut(s) 935, 1987
BcoDI GTCTC 3 cut(s) 674, 1611, 1985
BfaI CTAG 4 cut(s) 659, 1515, 1614, 2414
BfmI CTRYAG 3 cut(s) 862, 2247, 2604
BfoI RGCGCY 1 cut(s) 60
BfuAI ACCTGC 2 cut(s) 742, 2597
BfuI GTATCC 2 cut(s) 536, 558
Bme1390I CCNGG 3 cut(s) 935, 1987, 2399
Bme18I GGWCC 1 cut(s) 2102
BmgT120I GGNCC 5 cut(s) 2102, 2221, 2333, 2736, 2858
BmiI GGNNCC 9 cut(s) 144, 162, 351, 1764, 2050, 2103, 2374, 2396, 2859
BmrFI CCNGG 3 cut(s) 935, 1987, 2399
BmsI GCATC 3 cut(s) 1555, 2176, 2578
BoxI GACNNNNGTC 3 cut(s) 1977, 2100, 2570
BpmI CTGGAG 3 cut(s) 86, 710, 2774
Bpu10I CCTNAGC 1 cut(s) 576
BpuEI CTTGAG 6 cut(s) 794, 1143, 1561, 1790, 1922, 2522
BpuMI CCSGG 2 cut(s) 935, 1987
BsaI GGTCTC 1 cut(s) 674
BsaJI CCNNGG 6 cut(s) 38, 81, 595, 1649, 1985, 2561
BsaWI WCCGGW 1 cut(s) 2391
BsaXI ACNNNNNCTCC 6 cut(s) 1348, 1378, 2142, 2172, 2577, 2607
Bse118I RCCGGY 2 cut(s) 33, 1905
Bse1I ACTGG 2 cut(s) 1894, 2661
Bse21I CCTNAGG 2 cut(s) 2337, 2826
Bse3DI GCAATG 3 cut(s) 420, 570, 1735
BseAI TCCGGA 1 cut(s) 2391
BseBI CCWGG 1 cut(s) 2399
BseDI CCNNGG 6 cut(s) 38, 81, 595, 1649, 1985, 2561
BseMI GCAATG 3 cut(s) 420, 570, 1735
BseMII CTCAG 9 cut(s) 567, 891, 1460, 1920, 1956, 2097, 2351, 2664, 2817
BseNI ACTGG 2 cut(s) 1894, 2661
BseSI GKGCMC 1 cut(s) 2460
BseX3I CGGCCG 1 cut(s) 35
BseXI GCAGC 9 cut(s) 395, 705, 876, 917, 1596, 1599, 1619, 2422, 2485
BseYI CCCAGC 1 cut(s) 1758
Bsh1236I CGCG 2 cut(s) 482, 597
Bsh1285I CGRYCG 1 cut(s) 38
BshNI GGYRCC 1 cut(s) 2372
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 4 cut(s) 696, 810, 2460, 2526
BsiSI CCGG 7 cut(s) 34, 353, 934, 1906, 1987, 2093, 2392
BslFI GGGAC 1 cut(s) 2396
BsmAI GTCTC 3 cut(s) 674, 1611, 1985
BsmBI CGTCTC 2 cut(s) 1611, 1985
BsmFI GGGAC 1 cut(s) 2396
BsmI GAATGC 3 cut(s) 1377, 1847, 2821
Bso31I GGTCTC 1 cut(s) 674
Bsp1286I GDGCHC 4 cut(s) 696, 810, 2460, 2526
Bsp13I TCCGGA 1 cut(s) 2391
Bsp143I GATC 5 cut(s) 913, 1719, 1745, 2394, 2497
BspCNI CTCAG 9 cut(s) 568, 892, 1459, 1921, 1957, 2096, 2350, 2665, 2818
BspEI TCCGGA 1 cut(s) 2391
BspFNI CGCG 2 cut(s) 482, 597
BspLI GGNNCC 9 cut(s) 144, 162, 351, 1764, 2050, 2103, 2374, 2396, 2859
BspMAI CTGCAG 2 cut(s) 866, 2608
BspMI ACCTGC 2 cut(s) 742, 2597
BspPI GGATC 3 cut(s) 1753, 2389, 2402
BspQI GCTCTTC 2 cut(s) 1034, 1070
BspT107I GGYRCC 1 cut(s) 2372
BspTNI GGTCTC 1 cut(s) 674
BsrDI GCAATG 3 cut(s) 420, 570, 1735
BsrFI RCCGGY 2 cut(s) 33, 1905
BsrI ACTGG 2 cut(s) 1894, 2661
BssAI RCCGGY 2 cut(s) 33, 1905
BssECI CCNNGG 6 cut(s) 38, 81, 595, 1649, 1985, 2561
BssMI GATC 5 cut(s) 913, 1719, 1745, 2394, 2497
BssNAI GTATAC 2 cut(s) 536, 2031
BssSI CACGAG 1 cut(s) 2311
BssT1I CCWWGG 1 cut(s) 1649
Bst1107I GTATAC 2 cut(s) 536, 2031
Bst2BI CACGAG 1 cut(s) 2311
Bst2UI CCWGG 1 cut(s) 2399
Bst4CI ACNGT 5 cut(s) 698, 1622, 2462, 2506, 2518
BstAPI GCANNNNNTGC 3 cut(s) 618, 861, 1383
BstC8I GCNNGC 9 cut(s) 35, 619, 1046, 1082, 1512, 1782, 1907, 2740, 2778
BstDSI CCRYGG 2 cut(s) 595, 2561
BstFNI CGCG 2 cut(s) 482, 597
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 3 cut(s) 59, 482, 2874
BstKTI GATC 5 cut(s) 916, 1722, 1748, 2397, 2500
BstMAI GTCTC 3 cut(s) 674, 1611, 1985
BstMBI GATC 5 cut(s) 913, 1719, 1745, 2394, 2497
BstMCI CGRYCG 1 cut(s) 38
BstNI CCWGG 1 cut(s) 2399
BstNSI RCATGY 4 cut(s) 1514, 1530, 2456, 2780
BstPAI GACNNNNGTC 3 cut(s) 1977, 2100, 2570
BstSCI CCNGG 3 cut(s) 933, 1985, 2397
BstSFI CTRYAG 3 cut(s) 862, 2247, 2604
BstSLI GKGCMC 1 cut(s) 2460
BstUI CGCG 2 cut(s) 482, 597
BstV1I GCAGC 9 cut(s) 395, 705, 876, 917, 1596, 1599, 1619, 2422, 2485
BstX2I RGATCY 1 cut(s) 2394
BstXI CCANNNNNNTGG 1 cut(s) 2582
BstYI RGATCY 1 cut(s) 2394
BstZ17I GTATAC 2 cut(s) 536, 2031
BstZI CGGCCG 1 cut(s) 35
Bsu36I CCTNAGG 2 cut(s) 2337, 2826
BsuI GTATCC 2 cut(s) 536, 558
BtgI CCRYGG 2 cut(s) 595, 2561
BtsI GCAGTG 1 cut(s) 2170
BtsIMutI CAGTG 7 cut(s) 449, 694, 1697, 2170, 2458, 2514, 2654
BveI ACCTGC 2 cut(s) 742, 2597
Cac8I GCNNGC 9 cut(s) 35, 619, 1046, 1082, 1512, 1782, 1907, 2740, 2778
CaiI CAGNNNCTG 2 cut(s) 1154, 1590
CfoI GCGC 3 cut(s) 59, 482, 2874
Cfr10I RCCGGY 2 cut(s) 33, 1905
Cfr13I GGNCC 5 cut(s) 2102, 2221, 2333, 2736, 2858
Cfr42I CCGCGG 1 cut(s) 598
Csp6I GTAC 1 cut(s) 850
CviQI GTAC 1 cut(s) 850
DpnI GATC 5 cut(s) 915, 1721, 1747, 2396, 2499
DpnII GATC 5 cut(s) 913, 1719, 1745, 2394, 2497
EaeI YGGCCR 1 cut(s) 35
EagI CGGCCG 1 cut(s) 35
EciI GGCGGA 3 cut(s) 208, 407, 2712
Ecl136II GAGCTC 1 cut(s) 808
EclXI CGGCCG 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 1649
Eco24I GRGCYC 1 cut(s) 810
Eco31I GGTCTC 1 cut(s) 674
Eco47I GGWCC 1 cut(s) 2102
Eco52I CGGCCG 1 cut(s) 35
Eco53kI GAGCTC 1 cut(s) 808
Eco57I CTGAAG 4 cut(s) 1307, 1356, 2235, 2373
Eco81I CCTNAGG 2 cut(s) 2337, 2826
EcoICRI GAGCTC 1 cut(s) 808
EcoO109I RGGNCCY 1 cut(s) 2858
EcoRI GAATTC 1 cut(s) 2546
EcoRII CCWGG 1 cut(s) 2397
EcoT14I CCWWGG 1 cut(s) 1649
EcoT22I ATGCAT 1 cut(s) 1512
EcoT38I GRGCYC 1 cut(s) 810
ErhI CCWWGG 1 cut(s) 1649
Esp3I CGTCTC 2 cut(s) 1611, 1985
FalI AAGNNNNNCTT 4 cut(s) 606, 638, 2208, 2240
FaqI GGGAC 1 cut(s) 2396
FauI CCCGC 2 cut(s) 1249, 1962
FbaI TGATCA 1 cut(s) 2497
FblI GTMKAC 2 cut(s) 535, 2030
FriOI GRGCYC 1 cut(s) 810
FseI GGCCGGCC 2 cut(s) 37, 1909
FspBI CTAG 4 cut(s) 659, 1515, 1614, 2414
GlaI GCGC 3 cut(s) 58, 481, 2873
GsaI CCCAGC 1 cut(s) 1762
GsuI CTGGAG 3 cut(s) 86, 710, 2774
HaeII RGCGCY 1 cut(s) 60
HapII CCGG 7 cut(s) 34, 353, 934, 1906, 1987, 2093, 2392
HhaI GCGC 3 cut(s) 59, 482, 2874
Hin6I GCGC 3 cut(s) 57, 480, 2872
HinP1I GCGC 3 cut(s) 57, 480, 2872
HincII GTYRAC 1 cut(s) 1573
HindII GTYRAC 1 cut(s) 1573
HindIII AAGCTT 1 cut(s) 2165
HpaII CCGG 7 cut(s) 34, 353, 934, 1906, 1987, 2093, 2392
HphI GGTGA 8 cut(s) 236, 766, 1721, 2027, 2635, 2696, 2770, 2822
Hpy166II GTNNAC 8 cut(s) 296, 536, 1167, 1344, 1573, 2031, 2458, 2565
Hpy188III TCNNGA 9 cut(s) 65, 659, 899, 944, 1160, 1196, 1614, 2339, 2392
Hpy8I GTNNAC 8 cut(s) 296, 536, 1167, 1344, 1573, 2031, 2458, 2565
Hpy99I CGWCG 1 cut(s) 677
HpyAV CCTTC 7 cut(s) 70, 206, 632, 814, 1105, 1895, 2791
HpyCH4III ACNGT 5 cut(s) 698, 1622, 2462, 2506, 2518
HpyCH4IV ACGT 1 cut(s) 423
HpySE526I ACGT 1 cut(s) 423
HspAI GCGC 3 cut(s) 57, 480, 2872
Kpn2I TCCGGA 1 cut(s) 2391
KroI GCCGGC 2 cut(s) 33, 1905
KroNI GCCGGC 2 cut(s) 35, 1907
Ksp22I TGATCA 1 cut(s) 2497
KspI CCGCGG 1 cut(s) 598
Kzo9I GATC 5 cut(s) 913, 1719, 1745, 2394, 2497
LguI GCTCTTC 2 cut(s) 1034, 1070
LmnI GCTCC 8 cut(s) 110, 142, 166, 349, 691, 805, 2137, 2521
Lsp1109I GCAGC 9 cut(s) 395, 705, 876, 917, 1596, 1599, 1619, 2422, 2485
LweI GCATC 3 cut(s) 1555, 2176, 2578
MaeI CTAG 4 cut(s) 659, 1515, 1614, 2414
MaeII ACGT 1 cut(s) 423
MaeIII GTNAC 4 cut(s) 1496, 2158, 2306, 2479
MalI GATC 5 cut(s) 915, 1721, 1747, 2396, 2499
MboI GATC 5 cut(s) 913, 1719, 1745, 2394, 2497
MflI RGATCY 1 cut(s) 2394
MhlI GDGCHC 4 cut(s) 696, 810, 2460, 2526
MlyI GAGTC 6 cut(s) 164, 994, 1136, 1172, 1331, 1349
MmeI TCCRAC 1 cut(s) 1636
Mph1103I ATGCAT 1 cut(s) 1512
MroI TCCGGA 1 cut(s) 2391
MroNI GCCGGC 2 cut(s) 33, 1905
MroXI GAANNNNTTC 1 cut(s) 2709
MseI TTAA 5 cut(s) 791, 1251, 1551, 1598, 2193
MslI CAYNNNNRTG 4 cut(s) 1351, 1537, 2313, 2439
MspA1I CMGCKG 6 cut(s) 386, 597, 718, 1590, 2213, 2351
MspI CCGG 7 cut(s) 34, 353, 934, 1906, 1987, 2093, 2392
MspR9I CCNGG 3 cut(s) 935, 1987, 2399
Mva1269I GAATGC 3 cut(s) 1377, 1847, 2821
MvaI CCWGG 1 cut(s) 2399
MvnI CGCG 2 cut(s) 482, 597
NaeI GCCGGC 2 cut(s) 35, 1907
NciI CCSGG 2 cut(s) 935, 1987
NdeII GATC 5 cut(s) 913, 1719, 1745, 2394, 2497
NgoMIV GCCGGC 2 cut(s) 33, 1905
NlaIV GGNNCC 9 cut(s) 144, 162, 351, 1764, 2050, 2103, 2374, 2396, 2859
NmeAIII GCCGAG 2 cut(s) 63, 106
NmuCI GTSAC 2 cut(s) 2158, 2306
NsiI ATGCAT 1 cut(s) 1512
NspI RCATGY 4 cut(s) 1514, 1530, 2456, 2780
OliI CACNNNNGTG 2 cut(s) 1537, 2313
PaeI GCATGC 2 cut(s) 1514, 2780
PaqCI CACCTGC 1 cut(s) 742
PciSI GCTCTTC 2 cut(s) 1034, 1070
PctI GAATGC 3 cut(s) 1377, 1847, 2821
PdiI GCCGGC 2 cut(s) 35, 1907
PdmI GAANNNNTTC 1 cut(s) 2709
PfoI TCCNGGA 1 cut(s) 2397
PleI GAGTC 6 cut(s) 163, 993, 1136, 1171, 1330, 1348
PpsI GAGTC 6 cut(s) 163, 993, 1136, 1171, 1330, 1348
PshAI GACNNNNGTC 3 cut(s) 1977, 2100, 2570
Psp124BI GAGCTC 1 cut(s) 810
Psp6I CCWGG 1 cut(s) 2397
PspFI CCCAGC 1 cut(s) 1758
PspGI CCWGG 1 cut(s) 2397
PspN4I GGNNCC 9 cut(s) 144, 162, 351, 1764, 2050, 2103, 2374, 2396, 2859
PspPI GGNCC 5 cut(s) 2102, 2221, 2333, 2736, 2858
PstI CTGCAG 2 cut(s) 866, 2608
PstNI CAGNNNCTG 2 cut(s) 1154, 1590
PsuI RGATCY 1 cut(s) 2394
PvuII CAGCTG 4 cut(s) 718, 1590, 2213, 2351
RigI GGCCGGCC 2 cut(s) 37, 1909
RsaI GTAC 1 cut(s) 851
RsaNI GTAC 1 cut(s) 850
RseI CAYNNNNRTG 4 cut(s) 1351, 1537, 2313, 2439
SacI GAGCTC 1 cut(s) 810
SacII CCGCGG 1 cut(s) 598
SapI GCTCTTC 2 cut(s) 1034, 1070
SaqAI TTAA 5 cut(s) 791, 1251, 1551, 1598, 2193
Sau3AI GATC 5 cut(s) 913, 1719, 1745, 2394, 2497
Sau96I GGNCC 5 cut(s) 2102, 2221, 2333, 2736, 2858
SbfI CCTGCAGG 1 cut(s) 2608
SchI GAGTC 6 cut(s) 164, 994, 1136, 1172, 1331, 1349
ScrFI CCNGG 3 cut(s) 935, 1987, 2399
SdaI CCTGCAGG 1 cut(s) 2608
SduI GDGCHC 4 cut(s) 696, 810, 2460, 2526
SfaNI GCATC 3 cut(s) 1555, 2176, 2578
SfcI CTRYAG 3 cut(s) 862, 2247, 2604
Sfr303I CCGCGG 1 cut(s) 598
SgrBI CCGCGG 1 cut(s) 598
SinI GGWCC 1 cut(s) 2102
SmiMI CAYNNNNRTG 4 cut(s) 1351, 1537, 2313, 2439
SmlI CTYRAG 6 cut(s) 809, 1158, 1576, 1805, 1937, 2537
SmoI CTYRAG 6 cut(s) 809, 1158, 1576, 1805, 1937, 2537
SphI GCATGC 2 cut(s) 1514, 2780
Sse8387I CCTGCAGG 1 cut(s) 2608
SspI AATATT 2 cut(s) 1192, 2197
SspMI CTAG 4 cut(s) 659, 1515, 1614, 2414
SstI GAGCTC 1 cut(s) 810
StyD4I CCNGG 3 cut(s) 933, 1985, 2397
StyI CCWWGG 1 cut(s) 1649
TaaI ACNGT 5 cut(s) 698, 1622, 2462, 2506, 2518
TaiI ACGT 1 cut(s) 426
TaqI TCGA 8 cut(s) 625, 672, 771, 872, 943, 999, 2427, 2556
TatI WGTACW 1 cut(s) 849
TauI GCSGC 7 cut(s) 8, 193, 308, 389, 392, 597, 600
Tru1I TTAA 5 cut(s) 791, 1251, 1551, 1598, 2193
Tru9I TTAA 5 cut(s) 791, 1251, 1551, 1598, 2193
TscAI CASTG 7 cut(s) 449, 701, 1697, 2177, 2465, 2521, 2661
TseFI GTSAC 2 cut(s) 2158, 2306
TseI GCWGC 9 cut(s) 383, 718, 864, 930, 1584, 1587, 1607, 2410, 2473
Tsp45I GTSAC 2 cut(s) 2158, 2306
TspGWI ACGGA 2 cut(s) 714, 2717
TspRI CASTG 7 cut(s) 449, 701, 1697, 2177, 2465, 2521, 2661
VneI GTGCAC 1 cut(s) 2456
VpaK11BI GGWCC 1 cut(s) 2102
XapI RAATTY 4 cut(s) 1244, 2000, 2444, 2546
XbaI TCTAGA 2 cut(s) 658, 1613
XceI RCATGY 4 cut(s) 1514, 1530, 2456, 2780
XcmI CCANNNNNNNNNTGG 3 cut(s) 1773, 1843, 1886
XmiI GTMKAC 2 cut(s) 535, 2030
XmnI GAANNNNTTC 1 cut(s) 2709
XspI CTAG 4 cut(s) 659, 1515, 1614, 2414
Zsp2I ATGCAT 1 cut(s) 1512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.