Rh7CG305900

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000280
Physical Location & Seq
Forward (+)
32050234 .. 32060200
9967 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG305900.1

Sequence Viewer

Length: 3027 bp
ATGGCGAGAGGGGGAGGCAGAGGAAGAGGGAGATGGGCAAAGCGGCAAACCCATAACCCAAATCAAACTGAAGATGTTGCTGTCAAAGTTGGTGAAGATGGTGATGGGGTTGTTCTTTCTGAGATAAAGGCTGTGTCTTTTGGTGGTGTAGAGAAGGGTGAGGACGGTTTGGGAAACGATCAAAAGGAAGAAGTTGCTGTGAGTGTGAAGAAGAAGAAGAAGCCTGGGAGGAAGAGTAAGAAGGGTTTGGTGGGAAATGGTGATGGGGGTCTAAAGGAGGTTGCTTTTGGTGGTGATGAGATTTCTATGATGACTTTGAGGAAGAGGCCTGAACGGAACAAGAGAGTTAGAGTTATGGAGGCAGAGGGAAATGGTGAGAAAGGTAAAGAGAATGGTGCTTCTGTGAACAAGAGGGGTAGGAAAAAGGTGACATTTGCGAAGGAAGAGGGAAAGAAGGGTGTTTCAGAAGTGAATAATGACAAGAAGCGCGATAGGAAGCGTGCGAAAACTGAGGAGGAAGATGGGAATGAGGTAGGCTATTCGTTTAGGCCTCTGAGGGAGCAGGGACATCTATCAACCGAGGAGCGTAAAAAGTGGATTGAGGAGGTATCCCTAATGTGCCACCAATGTCAGAGGAATGATAAGGGAAGGGTTGTTCGGTGCAAAAAGTGCAGGACAAAGCGATATTGTGTTCCTTGCATAGAGAATTGGTATCCACATACATCAGAGGATGCCATTGCTGAGGCTTGTCCTGTATGCCGTGGAAATTGCAATTGCAAAGCATGCTTGCGCTTAGATATACCAGTTAAAAATCTGAAGAACCAAGATTTAGTACTTGACAAAGATGCAAGAATTGAGCACTCTAAGTACTTAATTCATGCACTGCTTCCATCCTTGAAAAGAATTCATGAAGAACAAGAGAATGAGATGGCGATGGAGGCGAGGAGAAAAGGATTATCTAACTTGAAGCTCCAGATACCAAAGTCTGACTGTGCTGCTGATGTTCGCGTGTATTGCAACAACTGCAGAACTTCTATTTTTGATTATCACAGAAGCTGTCCTAAATGTTCATATGACCTCTGCCTCATTTGTTGCCGGGAGATTCGAGAAGGACAATTGCAGCTAGGTAGGGAGGAAGTGACTTTGGAGCATGTTTCTCGGGGCTTACCTTATTTGCATGGTGGAGAGGAAGAGGACAACAAAAAAAGAAAGGGAAAGAGAAATAGGAATGTTGTGGAGCCACCCTCTGAAACTAGTCCCAAAAGTTCTGGAAAGCGAACATTTGAGTGGAAATCATATGAAGATGGAAGCATAATTTGCCCCCCAGAGGACATGGGAGGTTGTGGCAGTGGTAATCTAGAATTGAGATGCATGTTTTCCAAAAATTACGTCAAAGAGTTGGTAAAGAAAGCAGAAGAGATAGATGAAGCTTACAAACTTGTGTATTCAAGTAGAACTTCTGCAGAGAGGTGTTCATGTCTCAACTCTGTCGATGATGTCAATTCGAGCATCAATGCATCAAGGAAAGCAGCTTCACGAGACAATTCTGAGGACAACTATCTGTACTGTGCAAGGGCTGGAGATATCCAAGTAGAGGATTTAAAGCATTTCCAGTGGCATTGGATCAAGGGTGAGCCTGTTATCGTTAGCAATGCACTTGAAACTGCATCAGGTTTAAGCTGGGAACCATTGGTCATGTGGCGTGCTTGTCGTCAGATGCAACATACTAAGCATGGTAAACATTTGGACGTCAAAGCAATAGATTGCCTGGATTGGTGTGAGGGGGATATCAATATCCACCAATTTTTTACTGGATACTCAAAGGGACGGTTTGATAAAGAGAATTGGCCACAGATATTGAAACTGAAAGATTGGCCGCCAACTAATTTATTTGAGGAGCGCCTTCCTCGTCATGGTGCTGAGTTCATTTGTTGTCTGCCGTATAAGGAATATACTCATCCTCGCAATGGTCCTCTAAACCTTTTTGCCAAATTACCTTCAAAATGTGTAAAACCAGACATGGGACCAAAGACATATATTGCTTATGGTTTTTCCCAGGAGCTTGGACGTGGAGATTCTGTAACCAAGCTTCATTGCGATATGTCGGATGCGGTGAATGTTTTGACACATACCACTGAAGTGAACCTTACTCCTGAGAAACTTACTGTCATTGAAAAGTTGAAAAAGAAGCATGCTCAACAAGACCAAAGAGAAATTTTTGGCAATTGTCAGACTGTGGATGATGATGTTGATAGTGGCAAGGCTGCTGGTGATTCTTGTTTCAGTGCTGCTAATGATAAACAACTTCTTAATGAAGTTGATAATAATAGCAGTGGTGTAGCTGTTCCAGAACTGGTTGATCCAATTGTTCAACATGTTGGAGATTTTTCAACAGGATCTGTGTCCGAAAAGAATAAAGAGGCAGAATTAGATCATGATATCGGTGGTGAAATGCCTGCAAGAATTTATGGAAATAAGTTGGAAGAGTCGGATGTCAATGAGGGAGGTGCTTTATGGGACATATTCCGTAGACAGGATGTTCCTAAGCTGCAGGAGTATCTCATGAAGCATTTCAAGGAATTCAGGCACATACACTGTTGTCCATTGCAGCAGGTTATTCATCCCATACATGATCAGACATTTTATCTTACAATGGAGCACAAAAAGAAGCTTAAAGAGGAATATGGAATTGAACCATGGACCTTCATTCAAAACCTTGGAGATGCTGTCTTTATTCCTGCAGGATGTCCGCATCAAGTCAGGAACTTGAAGTCATGCATCAAAGTTGCAATGGACTTTGTCTCACCTGAAAATGTCGGCGAGTGCTTTCACTTGACAGAAGTATTCCGCACACTTCCAGAAGATCATAGGGCTAAAGAGGACAAATTGGAGGTAAAGAAAATGATTGTTCATTCTGTCACTGATGCTCTGGAGGAACTAAATCAGAATGTGAGGTCTAAGGAAGTCACTAATCCACCTCAATCAAGTCAGAGTGATGGAGATTATATGGTCCAGGATACTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1008

Amino Acids

114.18

Weight (kDa)

7.46

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 204 - 264 5.1e-07 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 836 - 933 7.7e-15 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1752
Acc36I ACCTGC 1 cut(s) 2602
AccI GTMKAC 1 cut(s) 2530
AccII CGCG 2 cut(s) 489, 1008
AciI CCGC 5 cut(s) 43, 1877, 2111, 2750, 2848
AclWI GGATC 3 cut(s) 1631, 2354, 2404
AcoI YGGCCR 2 cut(s) 1847, 1874
AcsI RAATTY 4 cut(s) 903, 2214, 2463, 2579
AcuI CTGAAG 3 cut(s) 90, 836, 2157
AcyI GRCGYC 1 cut(s) 1749
AfaI GTAC 3 cut(s) 834, 869, 1565
AfiI CCNNNNNNNGG 6 cut(s) 1327, 1594, 1913, 1967, 2021, 2533
AflIII ACRYGT 1 cut(s) 2374
AhlI ACTAGT 1 cut(s) 1253
AjiI CACGTC 1 cut(s) 2069
AjnI CCWGG 4 cut(s) 223, 1767, 2055, 3012
AleI CACNNNNGTG 1 cut(s) 2138
AloI GAACNNNNNNTCC 2 cut(s) 639, 671
Alw21I GWGCWC 2 cut(s) 861, 2661
Alw26I GTCTC 3 cut(s) 1484, 1533, 2806
AlwI GGATC 3 cut(s) 1631, 2354, 2404
AlwNI CAGNNNCTG 2 cut(s) 1056, 2399
Ama87I CYCGRG 1 cut(s) 1158
AoxI GGCC 4 cut(s) 326, 548, 1847, 1874
ApeKI GCWGC 7 cut(s) 995, 1120, 1529, 2264, 2288, 2548, 2608
ApoI RAATTY 4 cut(s) 903, 2214, 2463, 2579
ArsI GACNNNNNNTTYG 2 cut(s) 1374, 1406
Asp700I GAANNNNTTC 1 cut(s) 2570
AspLEI GCGC 3 cut(s) 489, 792, 1902
AspS9I GGNCC 4 cut(s) 1970, 2024, 2700, 3010
AsuC2I CCSGG 1 cut(s) 1097
AvaI CYCGRG 1 cut(s) 1158
AvaII GGWCC 4 cut(s) 1970, 2024, 2700, 3010
BaeI ACNNNNGTAYC 2 cut(s) 968, 1001
BalI TGGCCA 1 cut(s) 1849
BarI GAAGNNNNNNTAC 2 cut(s) 2073, 2105
BauI CACGAG 1 cut(s) 1536
Bbv12I GWGCWC 2 cut(s) 861, 2661
BbvCI CCTCAGC 1 cut(s) 741
BbvI GCAGC 7 cut(s) 982, 1132, 1541, 2251, 2275, 2535, 2620
BceAI ACGGC 2 cut(s) 744, 1924
BcgI CGANNNNNNTGC 2 cut(s) 1139, 1173
BciT130I CCWGG 4 cut(s) 225, 1769, 2057, 3014
BciVI GTATCC 4 cut(s) 619, 723, 1808, 3010
BclI TGATCA 1 cut(s) 2632
BcnI CCSGG 1 cut(s) 1097
BcoDI GTCTC 3 cut(s) 1484, 1533, 2806
BcuI ACTAGT 1 cut(s) 1253
BfaI CTAG 3 cut(s) 1124, 1254, 1358
BfmI CTRYAG 4 cut(s) 1024, 1461, 2549, 2739
BfoI RGCGCY 1 cut(s) 1903
BfuAI ACCTGC 1 cut(s) 2602
BfuI GTATCC 4 cut(s) 619, 723, 1808, 3010
BisI GCNGC 9 cut(s) 44, 996, 1121, 1530, 1877, 2265, 2289, 2549, 2609
BlsI GCNGC 9 cut(s) 45, 997, 1122, 1531, 1878, 2266, 2290, 2550, 2610
BmcAI AGTACT 2 cut(s) 834, 869
Bme1390I CCNGG 5 cut(s) 225, 1097, 1769, 2057, 3014
Bme18I GGWCC 4 cut(s) 1970, 2024, 2700, 3010
BmeT110I CYCGRG 1 cut(s) 1158
BmgBI CACGTC 1 cut(s) 2069
BmgT120I GGNCC 4 cut(s) 1970, 2024, 2700, 3010
BmiI GGNNCC 3 cut(s) 1239, 1686, 2025
BmrFI CCNGG 5 cut(s) 225, 1097, 1769, 2057, 3014
BplI GAGNNNNNCTC 2 cut(s) 1229, 1261
BpmI CTGGAG 3 cut(s) 956, 1599, 2951
Bpu10I CCTNAGC 2 cut(s) 741, 2544
BpuMI CCSGG 1 cut(s) 1097
BsaHI GRCGYC 1 cut(s) 1749
BsaJI CCNNGG 6 cut(s) 224, 579, 760, 2055, 2696, 2716
BsaXI ACNNNNNCTCC 6 cut(s) 220, 250, 2064, 2094, 2712, 2742
Bsc4I CCNNNNNNNGG 6 cut(s) 1327, 1594, 1913, 1967, 2021, 2533
Bse1I ACTGG 4 cut(s) 803, 1612, 1816, 2358
Bse3DI GCAATG 6 cut(s) 735, 1657, 1972, 2092, 2603, 2796
BseBI CCWGG 4 cut(s) 225, 1769, 2057, 3014
BseDI CCNNGG 6 cut(s) 224, 579, 760, 2055, 2696, 2716
BseGI GGATG 9 cut(s) 736, 890, 1957, 2113, 2245, 2497, 2542, 2620, 2750
BseLI CCNNNNNNNGG 6 cut(s) 1327, 1594, 1913, 1967, 2021, 2533
BseMI GCAATG 6 cut(s) 735, 1657, 1972, 2092, 2603, 2796
BseMII CTCAG 7 cut(s) 111, 501, 545, 732, 1539, 1911, 2145
BseNI ACTGG 4 cut(s) 803, 1612, 1816, 2358
BseRI GAGGAG 5 cut(s) 527, 596, 617, 958, 1910
BseXI GCAGC 7 cut(s) 982, 1132, 1541, 2251, 2275, 2535, 2620
BseYI CCCAGC 1 cut(s) 1680
BsgI GTGCAG 1 cut(s) 691
Bsh1236I CGCG 2 cut(s) 489, 1008
BshFI GGCC 4 cut(s) 328, 550, 1849, 1876
BsiHKAI GWGCWC 2 cut(s) 861, 2661
BsiHKCI CYCGRG 1 cut(s) 1158
BsiSI CCGG 1 cut(s) 1096
BslFI GGGAC 5 cut(s) 579, 1242, 1839, 2037, 2531
BslI CCNNNNNNNGG 6 cut(s) 1327, 1594, 1913, 1967, 2021, 2533
BsmAI GTCTC 3 cut(s) 1484, 1533, 2806
BsmFI GGGAC 5 cut(s) 579, 1242, 1839, 2037, 2531
BsnI GGCC 4 cut(s) 328, 550, 1849, 1876
BsoBI CYCGRG 1 cut(s) 1158
Bsp1286I GDGCHC 2 cut(s) 861, 2661
Bsp143I GATC 7 cut(s) 178, 1623, 2359, 2396, 2431, 2632, 2863
Bsp19I CCATGG 1 cut(s) 2696
BspACI CCGC 5 cut(s) 43, 1877, 2111, 2750, 2848
BspANI GGCC 4 cut(s) 328, 550, 1849, 1876
BspCNI CTCAG 7 cut(s) 112, 502, 546, 733, 1540, 1912, 2146
BspFNI CGCG 2 cut(s) 489, 1008
BspHI TCATGA 3 cut(s) 907, 2434, 2562
BspLI GGNNCC 3 cut(s) 1239, 1686, 2025
BspMAI CTGCAG 4 cut(s) 1028, 1465, 2553, 2743
BspMI ACCTGC 1 cut(s) 2602
BspPI GGATC 3 cut(s) 1631, 2354, 2404
BsrDI GCAATG 6 cut(s) 735, 1657, 1972, 2092, 2603, 2796
BsrI ACTGG 4 cut(s) 803, 1612, 1816, 2358
BssECI CCNNGG 6 cut(s) 224, 579, 760, 2055, 2696, 2716
BssMI GATC 7 cut(s) 178, 1623, 2359, 2396, 2431, 2632, 2863
BssNI GRCGYC 1 cut(s) 1749
BssSI CACGAG 1 cut(s) 1536
BssT1I CCWWGG 2 cut(s) 2696, 2716
Bst2BI CACGAG 1 cut(s) 1536
Bst2UI CCWGG 4 cut(s) 225, 1769, 2057, 3014
Bst4CI ACNGT 7 cut(s) 167, 992, 1568, 1830, 2167, 2236, 2597
Bst6I CTCTTC 7 cut(s) 19, 227, 317, 438, 1185, 1410, 2478
BstACI GRCGYC 1 cut(s) 1749
BstAPI GCANNNNNTGC 4 cut(s) 669, 783, 1023, 1317
BstC8I GCNNGC 6 cut(s) 501, 784, 788, 1704, 2193, 2457
BstDSI CCRYGG 2 cut(s) 760, 2696
BstF5I GGATG 9 cut(s) 736, 890, 1957, 2113, 2245, 2497, 2542, 2620, 2750
BstFNI CGCG 2 cut(s) 489, 1008
BstH2I RGCGCY 1 cut(s) 1903
BstHHI GCGC 3 cut(s) 489, 792, 1902
BstKTI GATC 7 cut(s) 181, 1626, 2362, 2399, 2434, 2635, 2866
BstMAI GTCTC 3 cut(s) 1484, 1533, 2806
BstMBI GATC 7 cut(s) 178, 1623, 2359, 2396, 2431, 2632, 2863
BstMWI GCNNNNNNNGC 6 cut(s) 669, 783, 938, 1014, 1023, 1317
BstNI CCWGG 4 cut(s) 225, 1769, 2057, 3014
BstNSI RCATGY 5 cut(s) 786, 1154, 1375, 2195, 2378
BstSCI CCNGG 5 cut(s) 223, 1095, 1767, 2055, 3012
BstSFI CTRYAG 4 cut(s) 1024, 1461, 2549, 2739
BstUI CGCG 2 cut(s) 489, 1008
BstV1I GCAGC 7 cut(s) 982, 1132, 1541, 2251, 2275, 2535, 2620
BstX2I RGATCY 1 cut(s) 2396
BstXI CCANNNNNNTGG 1 cut(s) 2063
BstYI RGATCY 1 cut(s) 2396
BsuI GTATCC 4 cut(s) 619, 723, 1808, 3010
BsuRI GGCC 4 cut(s) 328, 550, 1849, 1876
BtgI CCRYGG 2 cut(s) 760, 2696
BtgZI GCGATG 1 cut(s) 947
BtrI CACGTC 1 cut(s) 2069
BtsCI GGATG 9 cut(s) 736, 890, 1957, 2113, 2245, 2497, 2542, 2620, 2750
BtsI GCAGTG 3 cut(s) 881, 1354, 2338
BtsIMutI CAGTG 8 cut(s) 881, 1354, 1619, 2133, 2290, 2338, 2593, 2919
BveI ACCTGC 1 cut(s) 2602
Cac8I GCNNGC 6 cut(s) 501, 784, 788, 1704, 2193, 2457
CaiI CAGNNNCTG 2 cut(s) 1056, 2399
CciI TCATGA 3 cut(s) 907, 2434, 2562
CfoI GCGC 3 cut(s) 489, 792, 1902
Cfr13I GGNCC 4 cut(s) 1970, 2024, 2700, 3010
Csp6I GTAC 3 cut(s) 833, 868, 1564
CspCI CAANNNNNGTGG 2 cut(s) 1839, 1874
CviQI GTAC 3 cut(s) 833, 868, 1564
DpnI GATC 7 cut(s) 180, 1625, 2361, 2398, 2433, 2634, 2865
DpnII GATC 7 cut(s) 178, 1623, 2359, 2396, 2431, 2632, 2863
DraI TTTAAA 1 cut(s) 1602
EaeI YGGCCR 2 cut(s) 1847, 1874
Eam1104I CTCTTC 7 cut(s) 19, 227, 317, 438, 1185, 1410, 2478
EarI CTCTTC 7 cut(s) 19, 227, 317, 438, 1185, 1410, 2478
Eco130I CCWWGG 2 cut(s) 2696, 2716
Eco147I AGGCCT 2 cut(s) 328, 550
Eco32I GATATC 3 cut(s) 1585, 1789, 2440
Eco47I GGWCC 4 cut(s) 1970, 2024, 2700, 3010
Eco57I CTGAAG 3 cut(s) 90, 836, 2157
Eco88I CYCGRG 1 cut(s) 1158
EcoRI GAATTC 2 cut(s) 903, 2579
EcoRII CCWGG 4 cut(s) 223, 1767, 2055, 3012
EcoRV GATATC 3 cut(s) 1585, 1789, 2440
EcoT14I CCWWGG 2 cut(s) 2696, 2716
EcoT22I ATGCAT 3 cut(s) 1373, 1519, 2780
ErhI CCWWGG 2 cut(s) 2696, 2716
FalI AAGNNNNNCTT 4 cut(s) 1441, 1473, 2130, 2162
FaqI GGGAC 5 cut(s) 579, 1242, 1839, 2037, 2531
FauNDI CATATG 2 cut(s) 1072, 1297
FbaI TGATCA 1 cut(s) 2632
FblI GTMKAC 1 cut(s) 2530
Fnu4HI GCNGC 9 cut(s) 44, 996, 1121, 1530, 1877, 2265, 2289, 2549, 2609
FokI GGATG 9 cut(s) 743, 877, 1944, 2120, 2252, 2504, 2549, 2607, 2757
Fsp4HI GCNGC 9 cut(s) 44, 996, 1121, 1530, 1877, 2265, 2289, 2549, 2609
FspBI CTAG 3 cut(s) 1124, 1254, 1358
GlaI GCGC 3 cut(s) 488, 791, 1901
GluI GCNGC 9 cut(s) 44, 996, 1121, 1530, 1877, 2265, 2289, 2549, 2609
GsaI CCCAGC 1 cut(s) 1684
GsuI CTGGAG 3 cut(s) 956, 1599, 2951
HaeII RGCGCY 1 cut(s) 1903
HaeIII GGCC 4 cut(s) 328, 550, 1849, 1876
HapII CCGG 1 cut(s) 1096
HhaI GCGC 3 cut(s) 489, 792, 1902
Hin1I GRCGYC 1 cut(s) 1749
Hin6I GCGC 3 cut(s) 487, 790, 1900
HinP1I GCGC 3 cut(s) 487, 790, 1900
HindIII AAGCTT 3 cut(s) 1428, 2087, 2669
HinfI GANTC 4 cut(s) 1102, 2075, 2273, 2486
HpaII CCGG 1 cut(s) 1096
Hpy166II GTNNAC 4 cut(s) 406, 1739, 2143, 2531
Hpy8I GTNNAC 4 cut(s) 406, 1739, 2143, 2531
HpyAV CCTTC 9 cut(s) 148, 235, 433, 448, 642, 1103, 1913, 2007, 2713
HpyCH4III ACNGT 7 cut(s) 167, 992, 1568, 1830, 2167, 2236, 2597
HpyCH4IV ACGT 3 cut(s) 1389, 1749, 2068
HpyF10VI GCNNNNNNNGC 6 cut(s) 669, 783, 938, 1014, 1023, 1317
HpySE526I ACGT 3 cut(s) 1389, 1749, 2068
Hsp92I GRCGYC 1 cut(s) 1749
HspAI GCGC 3 cut(s) 487, 790, 1900
Ksp22I TGATCA 1 cut(s) 2632
Kzo9I GATC 7 cut(s) 178, 1623, 2359, 2396, 2431, 2632, 2863
LmnI GCTCC 8 cut(s) 559, 583, 975, 1147, 1237, 1897, 2059, 2656
Lsp1109I GCAGC 7 cut(s) 982, 1132, 1541, 2251, 2275, 2535, 2620
MaeI CTAG 3 cut(s) 1124, 1254, 1358
MaeII ACGT 3 cut(s) 1389, 1749, 2068
MaeIII GTNAC 5 cut(s) 427, 1138, 2080, 2917, 2965
MalI GATC 7 cut(s) 180, 1625, 2361, 2398, 2433, 2634, 2865
MboI GATC 7 cut(s) 178, 1623, 2359, 2396, 2431, 2632, 2863
MfeI CAATTG 4 cut(s) 772, 1115, 2224, 2364
MflI RGATCY 1 cut(s) 2396
MhlI GDGCHC 2 cut(s) 861, 2661
MlsI TGGCCA 1 cut(s) 1849
MluNI TGGCCA 1 cut(s) 1849
MlyI GAGTC 1 cut(s) 2495
MmeI TCCRAC 4 cut(s) 2085, 2359, 2460, 2469
Mox20I TGGCCA 1 cut(s) 1849
Mph1103I ATGCAT 3 cut(s) 1373, 1519, 2780
MroXI GAANNNNTTC 1 cut(s) 2570
MscI TGGCCA 1 cut(s) 1849
MseI TTAA 6 cut(s) 807, 872, 1601, 1676, 2310, 2673
MslI CAYNNNNRTG 2 cut(s) 1285, 2138
Msp20I TGGCCA 1 cut(s) 1849
MspI CCGG 1 cut(s) 1096
MspR9I CCNGG 5 cut(s) 225, 1097, 1769, 2057, 3014
MunI CAATTG 4 cut(s) 772, 1115, 2224, 2364
MvaI CCWGG 4 cut(s) 225, 1769, 2057, 3014
MvnI CGCG 2 cut(s) 489, 1008
MwoI GCNNNNNNNGC 6 cut(s) 669, 783, 938, 1014, 1023, 1317
NciI CCSGG 1 cut(s) 1097
NcoI CCATGG 1 cut(s) 2696
NdeI CATATG 2 cut(s) 1072, 1297
NdeII GATC 7 cut(s) 178, 1623, 2359, 2396, 2431, 2632, 2863
NlaIV GGNNCC 3 cut(s) 1239, 1686, 2025
NmuCI GTSAC 4 cut(s) 427, 1138, 2917, 2965
NsiI ATGCAT 3 cut(s) 1373, 1519, 2780
NspI RCATGY 5 cut(s) 786, 1154, 1375, 2195, 2378
OliI CACNNNNGTG 1 cut(s) 2138
PaeI GCATGC 2 cut(s) 786, 2195
PagI TCATGA 3 cut(s) 907, 2434, 2562
PceI AGGCCT 2 cut(s) 328, 550
PciI ACATGT 1 cut(s) 2374
PdmI GAANNNNTTC 1 cut(s) 2570
PfeI GAWTC 3 cut(s) 1102, 2075, 2273
PflFI GACNNNGTC 1 cut(s) 2798
PfoI TCCNGGA 1 cut(s) 3012
PkrI GCNGC 9 cut(s) 45, 997, 1122, 1531, 1878, 2266, 2290, 2550, 2610
PleI GAGTC 1 cut(s) 2494
PpsI GAGTC 1 cut(s) 2494
PscI ACATGT 1 cut(s) 2374
Psp6I CCWGG 4 cut(s) 223, 1767, 2055, 3012
PspFI CCCAGC 1 cut(s) 1680
PspGI CCWGG 4 cut(s) 223, 1767, 2055, 3012
PspN4I GGNNCC 3 cut(s) 1239, 1686, 2025
PspPI GGNCC 4 cut(s) 1970, 2024, 2700, 3010
PstI CTGCAG 4 cut(s) 1028, 1465, 2553, 2743
PstNI CAGNNNCTG 2 cut(s) 1056, 2399
PsuI RGATCY 1 cut(s) 2396
PsyI GACNNNGTC 1 cut(s) 2798
RsaI GTAC 3 cut(s) 834, 869, 1565
RsaNI GTAC 3 cut(s) 833, 868, 1564
RseI CAYNNNNRTG 2 cut(s) 1285, 2138
SaqAI TTAA 6 cut(s) 807, 872, 1601, 1676, 2310, 2673
SatI GCNGC 9 cut(s) 44, 996, 1121, 1530, 1877, 2265, 2289, 2549, 2609
Sau3AI GATC 7 cut(s) 178, 1623, 2359, 2396, 2431, 2632, 2863
Sau96I GGNCC 4 cut(s) 1970, 2024, 2700, 3010
SbfI CCTGCAGG 1 cut(s) 2743
ScaI AGTACT 2 cut(s) 834, 869
SchI GAGTC 1 cut(s) 2495
ScrFI CCNGG 5 cut(s) 225, 1097, 1769, 2057, 3014
SdaI CCTGCAGG 1 cut(s) 2743
SduI GDGCHC 2 cut(s) 861, 2661
SfcI CTRYAG 4 cut(s) 1024, 1461, 2549, 2739
SinI GGWCC 4 cut(s) 1970, 2024, 2700, 3010
SmiMI CAYNNNNRTG 2 cut(s) 1285, 2138
SpeI ACTAGT 1 cut(s) 1253
SphI GCATGC 2 cut(s) 786, 2195
Sse8387I CCTGCAGG 1 cut(s) 2743
SseBI AGGCCT 2 cut(s) 328, 550
SsiI CCGC 5 cut(s) 43, 1877, 2111, 2750, 2848
SspMI CTAG 3 cut(s) 1124, 1254, 1358
StuI AGGCCT 2 cut(s) 328, 550
StyD4I CCNGG 5 cut(s) 223, 1095, 1767, 2055, 3012
StyI CCWWGG 2 cut(s) 2696, 2716
TaaI ACNGT 7 cut(s) 167, 992, 1568, 1830, 2167, 2236, 2597
TaiI ACGT 3 cut(s) 1392, 1752, 2071
TaqI TCGA 3 cut(s) 1105, 1491, 1505
TatI WGTACW 3 cut(s) 832, 867, 1563
TauI GCSGC 2 cut(s) 46, 1879
TfiI GAWTC 3 cut(s) 1102, 2075, 2273
Tru1I TTAA 6 cut(s) 807, 872, 1601, 1676, 2310, 2673
Tru9I TTAA 6 cut(s) 807, 872, 1601, 1676, 2310, 2673
TscAI CASTG 8 cut(s) 888, 1354, 1619, 2140, 2290, 2338, 2600, 2926
TseFI GTSAC 4 cut(s) 427, 1138, 2917, 2965
TseI GCWGC 7 cut(s) 995, 1120, 1529, 2264, 2288, 2548, 2608
Tsp45I GTSAC 4 cut(s) 427, 1138, 2917, 2965
TspGWI ACGGA 2 cut(s) 349, 2516
TspRI CASTG 8 cut(s) 888, 1354, 1619, 2140, 2290, 2338, 2600, 2926
Tth111I GACNNNGTC 1 cut(s) 2798
VpaK11BI GGWCC 4 cut(s) 1970, 2024, 2700, 3010
XapI RAATTY 4 cut(s) 903, 2214, 2463, 2579
XbaI TCTAGA 1 cut(s) 1357
XceI RCATGY 5 cut(s) 786, 1154, 1375, 2195, 2378
XcmI CCANNNNNNNNNTGG 2 cut(s) 1695, 1808
XmiI GTMKAC 1 cut(s) 2530
XmnI GAANNNNTTC 1 cut(s) 2570
XspI CTAG 3 cut(s) 1124, 1254, 1358
ZraI GACGTC 1 cut(s) 1750
ZrmI AGTACT 2 cut(s) 834, 869
Zsp2I ATGCAT 3 cut(s) 1373, 1519, 2780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.