RchiOBHm_Chr7g0224011

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000280
Physical Location & Seq
Forward (+)
45872754 .. 45874702
1949 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20058

Sequence Viewer

Length: 1593 bp
ATGATCTGTGCCTCAGTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAAGAAGAGAGAGGTGGAGGAGGAAGCTCAAGTGTCCACATTTGAGTGGAGATTTAGTGAAGATGGAAGCATTCTCTGCCCCCCAACACAAAAGAAATAATCATGTCCTGGAATTGGTAGAGAAAGCAGGAAAAATAGATGCGAGTTACAATTTTTTGCCTGATAGTAGTGAAACATGTGCATACAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTCAAGCAGCAGCAGCTGTAAGTTAAGGAAAGCAGCTTCTAGAGTATCTTCTGATAGTAACTACTTGTTCTGTCCAAGGGTTGGGCAAATCCAAGATAAGGATTTTGGGCATTTTCAGTGGCATTGGATGAGGGGCGAGCCTGTGGTCGTTAGCAATGCATTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCCGTCATGTGGCGGGCACTTAGAAAGACAGTTAATAACTGCCATGCTAGTGAATTGACATTCCACGCCGTTGATTGCTGGGATTGGTGTTTTATAAGAATCAAAATCCGCCATTTCTTTAGTGGATATTCCAAGGGACGGTCTTATAAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATTATCTACTGAGTTTCACAATCGTCTTCCCCGGCATGGTAGGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATAGTCACCCCAACAAGGGAACTCTGAACCTTGCTACCTACTTGCCACCTACCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACTGGGAGATTCTGTAACCAAGCTTCACTGCAATATGTTTGATGCGGTTAATATTCTGACACATATAGCTGAAGTGGCCCTTACAACTGAACAACTTGCAACTATAGAGAAGTTGAAGAAAAAGCACAGGGAGCAAGACAAAAGAGAGAGATTTGGGAATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCTCTGCAAGAATTAGCTGAAGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGACTCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTATGCATGTGCTCTGTAATCCTTTGCAGCATGTTACTCATGCTATACATGATCAGACTGTTTATCTCACCGTGGAGCACAAGAGGAGGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAGCTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTGTCAAAGTTGCACTGGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCTGACAAACCACAGAGCCCATGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTGTGAAGGAGATTTCTGGTGAGAATGTGATGACTGAGGATGCGAAGTCTAGGGGAGGTGCAAGGAAATTAAGGCAAGGTGTAAGGCGCATGTCTTACAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

530

Amino Acids

60.38

Weight (kDa)

8.87

Isoelectric Point (pI)

47.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjC PF02373 357 - 453 8.8e-14 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 557, 609
Acc36I ACCTGC 1 cut(s) 1292
AccB1I GGYRCC 1 cut(s) 1067
AccB7I CCANNNNNTGG 1 cut(s) 350
AccIII TCCGGA 1 cut(s) 1086
AciI CCGC 4 cut(s) 475, 571, 884, 1103
AclWI GGATC 1 cut(s) 448
AcsI RAATTY 3 cut(s) 695, 1139, 1241
AcuI CTGAAG 2 cut(s) 930, 1068
AflIII ACRYGT 1 cut(s) 221
AgsI TTSAA 4 cut(s) 434, 718, 955, 1330
AjiI CACGTC 1 cut(s) 1132
AjnI CCWGG 1 cut(s) 153
AleI CACNNNNGTG 1 cut(s) 1008
Alw21I GWGCWC 2 cut(s) 1155, 1221
AlwI GGATC 1 cut(s) 448
AlwNI CAGNNNCTG 2 cut(s) 20, 285
Aor13HI TCCGGA 1 cut(s) 1086
AoxI GGCC 3 cut(s) 620, 915, 1028
ApeKI GCWGC 7 cut(s) 20, 276, 279, 282, 302, 1105, 1168
ApoI RAATTY 3 cut(s) 695, 1139, 1241
Asp700I GAANNNNTTC 1 cut(s) 1404
AspLEI GCGC 1 cut(s) 1575
AspS9I GGNCC 3 cut(s) 797, 916, 1028
AsuC2I CCSGG 2 cut(s) 25, 682
AsuHPI GGTGA 6 cut(s) 722, 1201, 1330, 1391, 1465, 1517
AvaII GGWCC 1 cut(s) 797
BaeGI GKGCMC 1 cut(s) 481
BanI GGYRCC 1 cut(s) 1067
BanII GRGCYC 1 cut(s) 1435
BarI GAAGNNNNNNTAC 2 cut(s) 846, 878
BbsI GAAGAC 1 cut(s) 668
Bbv12I GWGCWC 2 cut(s) 1155, 1221
BbvI GCAGC 7 cut(s) 7, 288, 291, 294, 314, 1117, 1180
BccI CCATC 5 cut(s) 31, 103, 469, 612, 1055
BceAI ACGGC 3 cut(s) 248, 515, 1240
BciT130I CCWGG 1 cut(s) 155
BclI TGATCA 1 cut(s) 1192
BcnI CCSGG 2 cut(s) 25, 682
BfaI CTAG 3 cut(s) 309, 510, 1536
BfmI CTRYAG 2 cut(s) 942, 1299
BfuAI ACCTGC 1 cut(s) 1292
BisI GCNGC 7 cut(s) 21, 277, 280, 283, 303, 1106, 1169
BlsI GCNGC 7 cut(s) 22, 278, 281, 284, 304, 1107, 1170
Bme1390I CCNGG 3 cut(s) 25, 155, 682
Bme18I GGWCC 1 cut(s) 797
BmgBI CACGTC 1 cut(s) 1132
BmgT120I GGNCC 3 cut(s) 797, 916, 1028
BmiI GGNNCC 3 cut(s) 459, 798, 1069
BmrFI CCNGG 3 cut(s) 25, 155, 682
BmrI ACTGGG 1 cut(s) 851
BmsI GCATC 5 cut(s) 175, 250, 871, 1273, 1516
BmuI ACTGGG 1 cut(s) 851
BoxI GACNNNNGTC 2 cut(s) 795, 1265
BpiI GAAGAC 1 cut(s) 668
BpmI CTGGAG 2 cut(s) 1130, 1469
BpuEI CTTGAG 4 cut(s) 59, 256, 617, 1217
BpuMI CCSGG 2 cut(s) 25, 682
BsaJI CCNNGG 5 cut(s) 344, 594, 680, 1212, 1256
BsaWI WCCGGW 1 cut(s) 1086
BsaXI ACNNNNNCTCC 6 cut(s) 87, 117, 837, 867, 1272, 1302
Bse1I ACTGG 2 cut(s) 846, 1356
Bse3DI GCAATG 1 cut(s) 430
BseAI TCCGGA 1 cut(s) 1086
BseBI CCWGG 1 cut(s) 155
BseDI CCNNGG 5 cut(s) 344, 594, 680, 1212, 1256
BseGI GGATG 8 cut(s) 262, 402, 461, 623, 1066, 1102, 1119, 1531
BseMI GCAATG 1 cut(s) 430
BseMII CTCAG 7 cut(s) 27, 638, 651, 792, 1106, 1359, 1512
BseNI ACTGG 2 cut(s) 846, 1356
BseRI GAGGAG 2 cut(s) 79, 1240
BseSI GKGCMC 1 cut(s) 481
BseXI GCAGC 7 cut(s) 7, 288, 291, 294, 314, 1117, 1180
BseYI CCCAGC 2 cut(s) 453, 540
BshFI GGCC 3 cut(s) 622, 917, 1030
BshNI GGYRCC 1 cut(s) 1067
BsiHKAI GWGCWC 2 cut(s) 1155, 1221
BsiSI CCGG 4 cut(s) 24, 682, 788, 1087
BslFI GGGAC 2 cut(s) 612, 1091
BsmFI GGGAC 2 cut(s) 612, 1091
BsmI GAATGC 1 cut(s) 116
BsnI GGCC 3 cut(s) 622, 917, 1030
Bsp1286I GDGCHC 4 cut(s) 481, 1155, 1221, 1435
Bsp13I TCCGGA 1 cut(s) 1086
Bsp143I GATC 3 cut(s) 3, 440, 1192
BspACI CCGC 4 cut(s) 475, 571, 884, 1103
BspANI GGCC 3 cut(s) 622, 917, 1030
BspCNI CTCAG 7 cut(s) 26, 637, 652, 791, 1105, 1360, 1513
BspEI TCCGGA 1 cut(s) 1086
BspLI GGNNCC 3 cut(s) 459, 798, 1069
BspMAI CTGCAG 1 cut(s) 1303
BspMI ACCTGC 1 cut(s) 1292
BspPI GGATC 1 cut(s) 448
BspT107I GGYRCC 1 cut(s) 1067
BsrDI GCAATG 1 cut(s) 430
BsrI ACTGG 2 cut(s) 846, 1356
BssECI CCNNGG 5 cut(s) 344, 594, 680, 1212, 1256
BssMI GATC 3 cut(s) 3, 440, 1192
BssT1I CCWWGG 2 cut(s) 344, 594
Bst2UI CCWGG 1 cut(s) 155
Bst4CI ACNGT 4 cut(s) 493, 603, 1201, 1213
Bst6I CTCTTC 2 cut(s) 46, 1395
BstAPI GCANNNNNTGC 1 cut(s) 122
BstC8I GCNNGC 3 cut(s) 407, 477, 1473
BstDEI CTNAG 8 cut(s) 13, 482, 624, 660, 778, 1092, 1368, 1521
BstDSI CCRYGG 2 cut(s) 1212, 1256
BstF5I GGATG 8 cut(s) 262, 402, 461, 623, 1066, 1102, 1119, 1531
BstHHI GCGC 1 cut(s) 1575
BstKTI GATC 3 cut(s) 6, 443, 1195
BstMBI GATC 3 cut(s) 3, 440, 1192
BstMWI GCNNNNNNNGC 6 cut(s) 17, 122, 276, 282, 914, 970
BstNI CCWGG 1 cut(s) 155
BstNSI RCATGY 5 cut(s) 225, 1151, 1175, 1475, 1579
BstPAI GACNNNNGTC 2 cut(s) 795, 1265
BstSCI CCNGG 3 cut(s) 23, 153, 680
BstSFI CTRYAG 2 cut(s) 942, 1299
BstSLI GKGCMC 1 cut(s) 481
BstV1I GCAGC 7 cut(s) 7, 288, 291, 294, 314, 1117, 1180
BstV2I GAAGAC 1 cut(s) 668
BstXI CCANNNNNNTGG 1 cut(s) 1277
BsuRI GGCC 3 cut(s) 622, 917, 1030
BtgI CCRYGG 2 cut(s) 1212, 1256
BtrI CACGTC 1 cut(s) 1132
BtsCI GGATG 8 cut(s) 262, 402, 461, 623, 1066, 1102, 1119, 1531
BtsI GCAGTG 1 cut(s) 865
BtsIMutI CAGTG 3 cut(s) 392, 865, 1349
BveI ACCTGC 1 cut(s) 1292
Cac8I GCNNGC 3 cut(s) 407, 477, 1473
CaiI CAGNNNCTG 2 cut(s) 20, 285
CfoI GCGC 1 cut(s) 1575
Cfr13I GGNCC 3 cut(s) 797, 916, 1028
DdeI CTNAG 8 cut(s) 13, 482, 624, 660, 778, 1092, 1368, 1521
DpnI GATC 3 cut(s) 5, 442, 1194
DpnII GATC 3 cut(s) 3, 440, 1192
Eam1104I CTCTTC 2 cut(s) 46, 1395
EarI CTCTTC 2 cut(s) 46, 1395
EciI GGCGGA 1 cut(s) 560
Eco130I CCWWGG 2 cut(s) 344, 594
Eco24I GRGCYC 1 cut(s) 1435
Eco47I GGWCC 1 cut(s) 797
Eco57I CTGAAG 2 cut(s) 930, 1068
EcoRI GAATTC 1 cut(s) 1241
EcoRII CCWGG 1 cut(s) 153
EcoT14I CCWWGG 2 cut(s) 344, 594
EcoT22I ATGCAT 2 cut(s) 430, 1149
EcoT38I GRGCYC 1 cut(s) 1435
ErhI CCWWGG 2 cut(s) 344, 594
FalI AAGNNNNNCTT 2 cut(s) 903, 935
FaqI GGGAC 2 cut(s) 612, 1091
FauI CCCGC 1 cut(s) 468
FbaI TGATCA 1 cut(s) 1192
Fnu4HI GCNGC 7 cut(s) 21, 277, 280, 283, 303, 1106, 1169
FokI GGATG 8 cut(s) 269, 409, 448, 630, 1073, 1106, 1109, 1538
FriOI GRGCYC 1 cut(s) 1435
Fsp4HI GCNGC 7 cut(s) 21, 277, 280, 283, 303, 1106, 1169
FspBI CTAG 3 cut(s) 309, 510, 1536
GlaI GCGC 1 cut(s) 1574
GluI GCNGC 7 cut(s) 21, 277, 280, 283, 303, 1106, 1169
GsaI CCCAGC 2 cut(s) 457, 544
GsuI CTGGAG 2 cut(s) 1130, 1469
HaeIII GGCC 3 cut(s) 622, 917, 1030
HapII CCGG 4 cut(s) 24, 682, 788, 1087
HhaI GCGC 1 cut(s) 1575
Hin6I GCGC 1 cut(s) 1573
HinP1I GCGC 1 cut(s) 1573
HindIII AAGCTT 2 cut(s) 860, 1274
HinfI GANTC 5 cut(s) 30, 561, 628, 848, 1090
HpaII CCGG 4 cut(s) 24, 682, 788, 1087
HphI GGTGA 6 cut(s) 722, 1201, 1330, 1391, 1465, 1517
Hpy166II GTNNAC 2 cut(s) 83, 1260
Hpy188I TCNGA 6 cut(s) 322, 627, 750, 897, 1197, 1419
Hpy188III TCNNGA 4 cut(s) 34, 309, 1087, 1094
Hpy8I GTNNAC 2 cut(s) 83, 1260
HpyAV CCTTC 1 cut(s) 1486
HpyCH4III ACNGT 4 cut(s) 493, 603, 1201, 1213
HpyCH4IV ACGT 1 cut(s) 1131
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 122, 276, 282, 914, 970
HpyF3I CTNAG 8 cut(s) 13, 482, 624, 660, 778, 1092, 1368, 1521
HpySE526I ACGT 1 cut(s) 1131
HspAI GCGC 1 cut(s) 1573
Kpn2I TCCGGA 1 cut(s) 1086
Ksp22I TGATCA 1 cut(s) 1192
Kzo9I GATC 3 cut(s) 3, 440, 1192
LmnI GCTCC 3 cut(s) 832, 970, 1216
Lsp1109I GCAGC 7 cut(s) 7, 288, 291, 294, 314, 1117, 1180
LweI GCATC 5 cut(s) 175, 250, 871, 1273, 1516
MaeI CTAG 3 cut(s) 309, 510, 1536
MaeII ACGT 1 cut(s) 1131
MaeIII GTNAC 6 cut(s) 191, 326, 728, 853, 1001, 1174
MalI GATC 3 cut(s) 5, 442, 1194
MboI GATC 3 cut(s) 3, 440, 1192
MboII GAAGA 8 cut(s) 60, 63, 118, 309, 668, 967, 1412, 1468
MhlI GDGCHC 4 cut(s) 481, 1155, 1221, 1435
MluCI AATT 9 cut(s) 158, 196, 515, 695, 997, 1040, 1139, 1241, 1553
MlyI GAGTC 1 cut(s) 1084
Mph1103I ATGCAT 2 cut(s) 430, 1149
MroI TCCGGA 1 cut(s) 1086
MroXI GAANNNNTTC 1 cut(s) 1404
MseI TTAA 6 cut(s) 246, 293, 449, 495, 888, 1556
MslI CAYNNNNRTG 5 cut(s) 90, 232, 510, 1008, 1134
MspA1I CMGCKG 2 cut(s) 285, 1108
MspI CCGG 4 cut(s) 24, 682, 788, 1087
MspR9I CCNGG 3 cut(s) 25, 155, 682
Mva1269I GAATGC 1 cut(s) 116
MvaI CCWGG 1 cut(s) 155
MwoI GCNNNNNNNGC 6 cut(s) 17, 122, 276, 282, 914, 970
NciI CCSGG 2 cut(s) 25, 682
NdeII GATC 3 cut(s) 3, 440, 1192
NlaIV GGNNCC 3 cut(s) 459, 798, 1069
NmuCI GTSAC 2 cut(s) 728, 1001
NsiI ATGCAT 2 cut(s) 430, 1149
NspI RCATGY 5 cut(s) 225, 1151, 1175, 1475, 1579
OliI CACNNNNGTG 1 cut(s) 1008
PaeI GCATGC 1 cut(s) 1475
PciI ACATGT 1 cut(s) 221
PctI GAATGC 1 cut(s) 116
PdmI GAANNNNTTC 1 cut(s) 1404
PfeI GAWTC 4 cut(s) 30, 561, 628, 848
PflMI CCANNNNNTGG 1 cut(s) 350
PfoI TCCNGGA 1 cut(s) 153
PkrI GCNGC 7 cut(s) 22, 278, 281, 284, 304, 1107, 1170
PleI GAGTC 1 cut(s) 1084
PpsI GAGTC 1 cut(s) 1084
PscI ACATGT 1 cut(s) 221
PshAI GACNNNNGTC 2 cut(s) 795, 1265
PsiI TTATAA 2 cut(s) 557, 609
Psp6I CCWGG 1 cut(s) 153
PspFI CCCAGC 2 cut(s) 453, 540
PspGI CCWGG 1 cut(s) 153
PspN4I GGNNCC 3 cut(s) 459, 798, 1069
PspPI GGNCC 3 cut(s) 797, 916, 1028
PstI CTGCAG 1 cut(s) 1303
PstNI CAGNNNCTG 2 cut(s) 20, 285
PvuII CAGCTG 2 cut(s) 285, 1108
RseI CAYNNNNRTG 5 cut(s) 90, 232, 510, 1008, 1134
SaqAI TTAA 6 cut(s) 246, 293, 449, 495, 888, 1556
SatI GCNGC 7 cut(s) 21, 277, 280, 283, 303, 1106, 1169
Sau3AI GATC 3 cut(s) 3, 440, 1192
Sau96I GGNCC 3 cut(s) 797, 916, 1028
SbfI CCTGCAGG 1 cut(s) 1303
SchI GAGTC 1 cut(s) 1084
ScrFI CCNGG 3 cut(s) 25, 155, 682
SdaI CCTGCAGG 1 cut(s) 1303
SduI GDGCHC 4 cut(s) 481, 1155, 1221, 1435
SfaNI GCATC 5 cut(s) 175, 250, 871, 1273, 1516
SfcI CTRYAG 2 cut(s) 942, 1299
SinI GGWCC 1 cut(s) 797
SmiMI CAYNNNNRTG 5 cut(s) 90, 232, 510, 1008, 1134
SmlI CTYRAG 4 cut(s) 74, 271, 632, 1232
SmoI CTYRAG 4 cut(s) 74, 271, 632, 1232
SphI GCATGC 1 cut(s) 1475
Sse8387I CCTGCAGG 1 cut(s) 1303
Sse9I AATT 9 cut(s) 158, 196, 515, 695, 997, 1040, 1139, 1241, 1553
SsiI CCGC 4 cut(s) 475, 571, 884, 1103
SspI AATATT 1 cut(s) 892
SspMI CTAG 3 cut(s) 309, 510, 1536
StyD4I CCNGG 3 cut(s) 23, 153, 680
StyI CCWWGG 2 cut(s) 344, 594
TaaI ACNGT 4 cut(s) 493, 603, 1201, 1213
TaiI ACGT 1 cut(s) 1134
TaqI TCGA 2 cut(s) 33, 1251
TasI AATT 9 cut(s) 158, 196, 515, 695, 997, 1040, 1139, 1241, 1553
TfiI GAWTC 4 cut(s) 30, 561, 628, 848
Tru1I TTAA 6 cut(s) 246, 293, 449, 495, 888, 1556
Tru9I TTAA 6 cut(s) 246, 293, 449, 495, 888, 1556
TscAI CASTG 3 cut(s) 392, 872, 1356
TseFI GTSAC 2 cut(s) 728, 1001
TseI GCWGC 7 cut(s) 20, 276, 279, 282, 302, 1105, 1168
Tsp45I GTSAC 2 cut(s) 728, 1001
TspDTI ATGAA 3 cut(s) 228, 1152, 1282
TspGWI ACGGA 2 cut(s) 454, 1412
TspRI CASTG 3 cut(s) 392, 872, 1356
Van91I CCANNNNNTGG 1 cut(s) 350
VpaK11BI GGWCC 1 cut(s) 797
XapI RAATTY 3 cut(s) 695, 1139, 1241
XbaI TCTAGA 1 cut(s) 308
XceI RCATGY 5 cut(s) 225, 1151, 1175, 1475, 1579
XcmI CCANNNNNNNNNTGG 2 cut(s) 468, 581
XmnI GAANNNNTTC 1 cut(s) 1404
XspI CTAG 3 cut(s) 309, 510, 1536
Zsp2I ATGCAT 2 cut(s) 430, 1149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.