lysine-specific demethylase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 796 |
| Acc36I | ACCTGC | 2 cut(s) | 796, 2654 |
| AccB1I | GGYRCC | 1 cut(s) | 2429 |
| AccI | GTMKAC | 1 cut(s) | 2087 |
| AccII | CGCG | 2 cut(s) | 274, 651 |
| AccIII | TCCGGA | 1 cut(s) | 2448 |
| AclWI | GGATC | 3 cut(s) | 1810, 2446, 2459 |
| AcoI | YGGCCR | 1 cut(s) | 35 |
| AcsI | RAATTY | 4 cut(s) | 1301, 2057, 2501, 2603 |
| AcuI | CTGAAG | 5 cut(s) | 270, 1364, 1413, 2292, 2430 |
| AfaI | GTAC | 1 cut(s) | 905 |
| AflIII | ACRYGT | 2 cut(s) | 272, 1583 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1201 |
| AjnI | CCWGG | 1 cut(s) | 2454 |
| AleI | CACNNNNGTG | 2 cut(s) | 1594, 2370 |
| Alw21I | GWGCWC | 6 cut(s) | 536, 750, 864, 1591, 2517, 2583 |
| Alw26I | GTCTC | 3 cut(s) | 728, 1668, 2042 |
| Alw44I | GTGCAC | 3 cut(s) | 532, 1587, 2513 |
| AlwI | GGATC | 3 cut(s) | 1810, 2446, 2459 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1211, 1647 |
| Aor13HI | TCCGGA | 1 cut(s) | 2448 |
| ApaLI | GTGCAC | 3 cut(s) | 532, 1587, 2513 |
| ApoI | RAATTY | 4 cut(s) | 1301, 2057, 2501, 2603 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2766 |
| AspLEI | GCGC | 3 cut(s) | 59, 887, 2934 |
| AspS9I | GGNCC | 6 cut(s) | 715, 2159, 2278, 2390, 2793, 2915 |
| AsuC2I | CCSGG | 2 cut(s) | 989, 2044 |
| AsuHPI | GGTGA | 7 cut(s) | 221, 820, 1778, 2084, 2692, 2827, 2879 |
| AvaII | GGWCC | 2 cut(s) | 715, 2159 |
| AxyI | CCTNAGG | 2 cut(s) | 2394, 2883 |
| BaeGI | GKGCMC | 3 cut(s) | 536, 1591, 2517 |
| BamHI | GGATCC | 1 cut(s) | 2451 |
| BanI | GGYRCC | 1 cut(s) | 2429 |
| BanII | GRGCYC | 1 cut(s) | 864 |
| BauI | CACGAG | 2 cut(s) | 535, 1225 |
| BbsI | GAAGAC | 1 cut(s) | 201 |
| Bbv12I | GWGCWC | 6 cut(s) | 536, 750, 864, 1591, 2517, 2583 |
| BbvCI | CCTCAGC | 1 cut(s) | 630 |
| BceAI | ACGGC | 2 cut(s) | 70, 2602 |
| BciT130I | CCWGG | 1 cut(s) | 2456 |
| BciVI | GTATCC | 2 cut(s) | 590, 612 |
| BclI | TGATCA | 1 cut(s) | 2554 |
| BcnI | CCSGG | 2 cut(s) | 989, 2044 |
| BcoDI | GTCTC | 3 cut(s) | 728, 1668, 2042 |
| BfaI | CTAG | 3 cut(s) | 1139, 1572, 1671 |
| BfmI | CTRYAG | 3 cut(s) | 916, 2304, 2661 |
| BfoI | RGCGCY | 1 cut(s) | 60 |
| BfuAI | ACCTGC | 2 cut(s) | 796, 2654 |
| BfuI | GTATCC | 2 cut(s) | 590, 612 |
| Bme1390I | CCNGG | 3 cut(s) | 989, 2044, 2456 |
| Bme18I | GGWCC | 2 cut(s) | 715, 2159 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1201 |
| BmgT120I | GGNCC | 6 cut(s) | 715, 2159, 2278, 2390, 2793, 2915 |
| BmrFI | CCNGG | 3 cut(s) | 989, 2044, 2456 |
| BmsI | GCATC | 3 cut(s) | 1612, 2233, 2635 |
| BoxI | GACNNNNGTC | 3 cut(s) | 2034, 2157, 2627 |
| BpiI | GAAGAC | 1 cut(s) | 201 |
| BpmI | CTGGAG | 4 cut(s) | 86, 764, 2492, 2831 |
| Bpu10I | CCTNAGC | 1 cut(s) | 630 |
| BpuEI | CTTGAG | 6 cut(s) | 848, 1200, 1618, 1847, 1979, 2579 |
| BpuMI | CCSGG | 2 cut(s) | 989, 2044 |
| BsaI | GGTCTC | 1 cut(s) | 728 |
| BsaJI | CCNNGG | 6 cut(s) | 38, 81, 649, 1706, 2042, 2618 |
| BsaWI | WCCGGW | 1 cut(s) | 2448 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 1405, 1435, 2199, 2229, 2634, 2664 |
| Bse118I | RCCGGY | 2 cut(s) | 33, 1962 |
| Bse1I | ACTGG | 3 cut(s) | 687, 1951, 2718 |
| Bse21I | CCTNAGG | 2 cut(s) | 2394, 2883 |
| Bse3DI | GCAATG | 2 cut(s) | 624, 1792 |
| BseAI | TCCGGA | 1 cut(s) | 2448 |
| BseBI | CCWGG | 1 cut(s) | 2456 |
| BseDI | CCNNGG | 6 cut(s) | 38, 81, 649, 1706, 2042, 2618 |
| BseGI | GGATG | 9 cut(s) | 691, 1291, 1333, 1764, 1985, 2428, 2464, 2481, 2893 |
| BseMI | GCAATG | 2 cut(s) | 624, 1792 |
| BseMII | CTCAG | 9 cut(s) | 621, 945, 1517, 1977, 2013, 2154, 2408, 2721, 2874 |
| BseNI | ACTGG | 3 cut(s) | 687, 1951, 2718 |
| BseSI | GKGCMC | 3 cut(s) | 536, 1591, 2517 |
| BseX3I | CGGCCG | 1 cut(s) | 35 |
| BseYI | CCCAGC | 1 cut(s) | 1815 |
| Bsh1236I | CGCG | 2 cut(s) | 274, 651 |
| Bsh1285I | CGRYCG | 1 cut(s) | 38 |
| BshNI | GGYRCC | 1 cut(s) | 2429 |
| BsiEI | CGRYCG | 1 cut(s) | 38 |
| BsiHKAI | GWGCWC | 6 cut(s) | 536, 750, 864, 1591, 2517, 2583 |
| BsiSI | CCGG | 8 cut(s) | 34, 344, 404, 988, 1963, 2044, 2150, 2449 |
| BslFI | GGGAC | 2 cut(s) | 812, 2453 |
| BsmAI | GTCTC | 3 cut(s) | 728, 1668, 2042 |
| BsmBI | CGTCTC | 1 cut(s) | 2042 |
| BsmFI | GGGAC | 2 cut(s) | 812, 2453 |
| BsmI | GAATGC | 2 cut(s) | 1904, 2878 |
| Bso31I | GGTCTC | 1 cut(s) | 728 |
| Bsp1286I | GDGCHC | 6 cut(s) | 536, 750, 864, 1591, 2517, 2583 |
| Bsp13I | TCCGGA | 1 cut(s) | 2448 |
| Bsp143I | GATC | 5 cut(s) | 967, 1776, 1802, 2451, 2554 |
| BspCNI | CTCAG | 9 cut(s) | 622, 946, 1516, 1978, 2014, 2153, 2407, 2722, 2875 |
| BspEI | TCCGGA | 1 cut(s) | 2448 |
| BspFNI | CGCG | 2 cut(s) | 274, 651 |
| BspMAI | CTGCAG | 2 cut(s) | 920, 2665 |
| BspMI | ACCTGC | 2 cut(s) | 796, 2654 |
| BspPI | GGATC | 3 cut(s) | 1810, 2446, 2459 |
| BspQI | GCTCTTC | 2 cut(s) | 1088, 1124 |
| BspT107I | GGYRCC | 1 cut(s) | 2429 |
| BspTNI | GGTCTC | 1 cut(s) | 728 |
| BsrDI | GCAATG | 2 cut(s) | 624, 1792 |
| BsrFI | RCCGGY | 2 cut(s) | 33, 1962 |
| BsrI | ACTGG | 3 cut(s) | 687, 1951, 2718 |
| BssAI | RCCGGY | 2 cut(s) | 33, 1962 |
| BssECI | CCNNGG | 6 cut(s) | 38, 81, 649, 1706, 2042, 2618 |
| BssMI | GATC | 5 cut(s) | 967, 1776, 1802, 2451, 2554 |
| BssNAI | GTATAC | 1 cut(s) | 2088 |
| BssSI | CACGAG | 2 cut(s) | 535, 1225 |
| BssT1I | CCWWGG | 1 cut(s) | 1706 |
| Bst1107I | GTATAC | 1 cut(s) | 2088 |
| Bst2BI | CACGAG | 2 cut(s) | 535, 1225 |
| Bst2UI | CCWGG | 1 cut(s) | 2456 |
| Bst4CI | ACNGT | 6 cut(s) | 200, 752, 1679, 2519, 2563, 2575 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 672, 915, 1440 |
| BstC8I | GCNNGC | 9 cut(s) | 35, 673, 1100, 1136, 1569, 1839, 1964, 2797, 2835 |
| BstDSI | CCRYGG | 2 cut(s) | 649, 2618 |
| BstF5I | GGATG | 9 cut(s) | 691, 1291, 1333, 1764, 1985, 2428, 2464, 2481, 2893 |
| BstFNI | CGCG | 2 cut(s) | 274, 651 |
| BstH2I | RGCGCY | 1 cut(s) | 60 |
| BstHHI | GCGC | 3 cut(s) | 59, 887, 2934 |
| BstKTI | GATC | 5 cut(s) | 970, 1779, 1805, 2454, 2557 |
| BstMAI | GTCTC | 3 cut(s) | 728, 1668, 2042 |
| BstMBI | GATC | 5 cut(s) | 967, 1776, 1802, 2451, 2554 |
| BstMCI | CGRYCG | 1 cut(s) | 38 |
| BstNI | CCWGG | 1 cut(s) | 2456 |
| BstNSI | RCATGY | 4 cut(s) | 1571, 1587, 2513, 2837 |
| BstPAI | GACNNNNGTC | 3 cut(s) | 2034, 2157, 2627 |
| BstSCI | CCNGG | 3 cut(s) | 987, 2042, 2454 |
| BstSFI | CTRYAG | 3 cut(s) | 916, 2304, 2661 |
| BstSLI | GKGCMC | 3 cut(s) | 536, 1591, 2517 |
| BstUI | CGCG | 2 cut(s) | 274, 651 |
| BstV2I | GAAGAC | 1 cut(s) | 201 |
| BstX2I | RGATCY | 1 cut(s) | 2451 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2639 |
| BstYI | RGATCY | 1 cut(s) | 2451 |
| BstZ17I | GTATAC | 1 cut(s) | 2088 |
| BstZI | CGGCCG | 1 cut(s) | 35 |
| Bsu36I | CCTNAGG | 2 cut(s) | 2394, 2883 |
| BsuI | GTATCC | 2 cut(s) | 590, 612 |
| BtgI | CCRYGG | 2 cut(s) | 649, 2618 |
| BtsCI | GGATG | 9 cut(s) | 691, 1291, 1333, 1764, 1985, 2428, 2464, 2481, 2893 |
| BtsI | GCAGTG | 3 cut(s) | 384, 474, 2227 |
| BveI | ACCTGC | 2 cut(s) | 796, 2654 |
| Cac8I | GCNNGC | 9 cut(s) | 35, 673, 1100, 1136, 1569, 1839, 1964, 2797, 2835 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1211, 1647 |
| CfoI | GCGC | 3 cut(s) | 59, 887, 2934 |
| Cfr10I | RCCGGY | 2 cut(s) | 33, 1962 |
| Cfr13I | GGNCC | 6 cut(s) | 715, 2159, 2278, 2390, 2793, 2915 |
| Cfr42I | CCGCGG | 1 cut(s) | 652 |
| CseI | GACGC | 1 cut(s) | 280 |
| Csp6I | GTAC | 1 cut(s) | 904 |
| CviQI | GTAC | 1 cut(s) | 904 |
| DpnI | GATC | 5 cut(s) | 969, 1778, 1804, 2453, 2556 |
| DpnII | GATC | 5 cut(s) | 967, 1776, 1802, 2451, 2554 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1201 |
| EaeI | YGGCCR | 1 cut(s) | 35 |
| EagI | CGGCCG | 1 cut(s) | 35 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1201 |
| EciI | GGCGGA | 3 cut(s) | 190, 461, 2769 |
| Ecl136II | GAGCTC | 1 cut(s) | 862 |
| EclXI | CGGCCG | 1 cut(s) | 35 |
| Eco130I | CCWWGG | 1 cut(s) | 1706 |
| Eco24I | GRGCYC | 1 cut(s) | 864 |
| Eco31I | GGTCTC | 1 cut(s) | 728 |
| Eco47I | GGWCC | 2 cut(s) | 715, 2159 |
| Eco52I | CGGCCG | 1 cut(s) | 35 |
| Eco53kI | GAGCTC | 1 cut(s) | 862 |
| Eco57I | CTGAAG | 5 cut(s) | 270, 1364, 1413, 2292, 2430 |
| Eco81I | CCTNAGG | 2 cut(s) | 2394, 2883 |
| EcoICRI | GAGCTC | 1 cut(s) | 862 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2915 |
| EcoRI | GAATTC | 1 cut(s) | 2603 |
| EcoRII | CCWGG | 1 cut(s) | 2454 |
| EcoT14I | CCWWGG | 1 cut(s) | 1706 |
| EcoT22I | ATGCAT | 1 cut(s) | 1569 |
| EcoT38I | GRGCYC | 1 cut(s) | 864 |
| ErhI | CCWWGG | 1 cut(s) | 1706 |
| Esp3I | CGTCTC | 1 cut(s) | 2042 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 660, 692, 2265, 2297 |
| FaqI | GGGAC | 2 cut(s) | 812, 2453 |
| FauI | CCCGC | 2 cut(s) | 1306, 2019 |
| FbaI | TGATCA | 1 cut(s) | 2554 |
| FblI | GTMKAC | 1 cut(s) | 2087 |
| FokI | GGATG | 9 cut(s) | 698, 1298, 1340, 1771, 1992, 2435, 2468, 2471, 2900 |
| FriOI | GRGCYC | 1 cut(s) | 864 |
| FseI | GGCCGGCC | 2 cut(s) | 37, 1966 |
| FspBI | CTAG | 3 cut(s) | 1139, 1572, 1671 |
| GlaI | GCGC | 3 cut(s) | 58, 886, 2933 |
| GsaI | CCCAGC | 1 cut(s) | 1819 |
| GsuI | CTGGAG | 4 cut(s) | 86, 764, 2492, 2831 |
| HaeII | RGCGCY | 1 cut(s) | 60 |
| HapII | CCGG | 8 cut(s) | 34, 344, 404, 988, 1963, 2044, 2150, 2449 |
| HgaI | GACGC | 1 cut(s) | 280 |
| HhaI | GCGC | 3 cut(s) | 59, 887, 2934 |
| Hin6I | GCGC | 3 cut(s) | 57, 885, 2932 |
| HinP1I | GCGC | 3 cut(s) | 57, 885, 2932 |
| HincII | GTYRAC | 1 cut(s) | 1630 |
| HindII | GTYRAC | 1 cut(s) | 1630 |
| HindIII | AAGCTT | 1 cut(s) | 2222 |
| HpaII | CCGG | 8 cut(s) | 34, 344, 404, 988, 1963, 2044, 2150, 2449 |
| HphI | GGTGA | 7 cut(s) | 221, 820, 1778, 2084, 2692, 2827, 2879 |
| Hpy166II | GTNNAC | 8 cut(s) | 534, 1224, 1401, 1589, 1630, 2088, 2515, 2622 |
| Hpy188III | TCNNGA | 9 cut(s) | 65, 713, 953, 998, 1217, 1253, 1671, 2396, 2449 |
| Hpy8I | GTNNAC | 8 cut(s) | 534, 1224, 1401, 1589, 1630, 2088, 2515, 2622 |
| Hpy99I | CGWCG | 1 cut(s) | 1204 |
| HpyAV | CCTTC | 7 cut(s) | 70, 466, 686, 868, 1159, 1952, 2848 |
| HpyCH4III | ACNGT | 6 cut(s) | 200, 752, 1679, 2519, 2563, 2575 |
| HpyCH4IV | ACGT | 1 cut(s) | 477 |
| HpySE526I | ACGT | 1 cut(s) | 477 |
| HspAI | GCGC | 3 cut(s) | 57, 885, 2932 |
| Kpn2I | TCCGGA | 1 cut(s) | 2448 |
| KroI | GCCGGC | 2 cut(s) | 33, 1962 |
| KroNI | GCCGGC | 2 cut(s) | 35, 1964 |
| Ksp22I | TGATCA | 1 cut(s) | 2554 |
| KspI | CCGCGG | 1 cut(s) | 652 |
| Kzo9I | GATC | 5 cut(s) | 967, 1776, 1802, 2451, 2554 |
| LguI | GCTCTTC | 2 cut(s) | 1088, 1124 |
| LmnI | GCTCC | 7 cut(s) | 110, 148, 340, 400, 745, 2194, 2578 |
| LweI | GCATC | 3 cut(s) | 1612, 2233, 2635 |
| MaeI | CTAG | 3 cut(s) | 1139, 1572, 1671 |
| MaeII | ACGT | 1 cut(s) | 477 |
| MaeIII | GTNAC | 4 cut(s) | 1553, 2215, 2363, 2536 |
| MalI | GATC | 5 cut(s) | 969, 1778, 1804, 2453, 2556 |
| MboI | GATC | 5 cut(s) | 967, 1776, 1802, 2451, 2554 |
| MflI | RGATCY | 1 cut(s) | 2451 |
| MhlI | GDGCHC | 6 cut(s) | 536, 750, 864, 1591, 2517, 2583 |
| MluI | ACGCGT | 1 cut(s) | 272 |
| MlyI | GAGTC | 5 cut(s) | 1048, 1190, 1229, 1388, 1406 |
| MmeI | TCCRAC | 1 cut(s) | 1693 |
| Mph1103I | ATGCAT | 1 cut(s) | 1569 |
| MroI | TCCGGA | 1 cut(s) | 2448 |
| MroNI | GCCGGC | 2 cut(s) | 33, 1962 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2766 |
| MseI | TTAA | 6 cut(s) | 266, 845, 1308, 1608, 1655, 2250 |
| MslI | CAYNNNNRTG | 4 cut(s) | 1408, 1594, 2370, 2496 |
| MspA1I | CMGCKG | 7 cut(s) | 440, 651, 772, 1647, 2270, 2408, 2470 |
| MspI | CCGG | 8 cut(s) | 34, 344, 404, 988, 1963, 2044, 2150, 2449 |
| MspR9I | CCNGG | 3 cut(s) | 989, 2044, 2456 |
| Mva1269I | GAATGC | 2 cut(s) | 1904, 2878 |
| MvaI | CCWGG | 1 cut(s) | 2456 |
| MvnI | CGCG | 2 cut(s) | 274, 651 |
| NaeI | GCCGGC | 2 cut(s) | 35, 1964 |
| NciI | CCSGG | 2 cut(s) | 989, 2044 |
| NdeII | GATC | 5 cut(s) | 967, 1776, 1802, 2451, 2554 |
| NgoMIV | GCCGGC | 2 cut(s) | 33, 1962 |
| NmeAIII | GCCGAG | 2 cut(s) | 63, 106 |
| NmuCI | GTSAC | 2 cut(s) | 2215, 2363 |
| NsiI | ATGCAT | 1 cut(s) | 1569 |
| NspI | RCATGY | 4 cut(s) | 1571, 1587, 2513, 2837 |
| OliI | CACNNNNGTG | 2 cut(s) | 1594, 2370 |
| PaeI | GCATGC | 2 cut(s) | 1571, 2837 |
| PaqCI | CACCTGC | 1 cut(s) | 796 |
| PciI | ACATGT | 1 cut(s) | 1583 |
| PciSI | GCTCTTC | 2 cut(s) | 1088, 1124 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 542 |
| PctI | GAATGC | 2 cut(s) | 1904, 2878 |
| PdiI | GCCGGC | 2 cut(s) | 35, 1964 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2766 |
| PfoI | TCCNGGA | 1 cut(s) | 2454 |
| PleI | GAGTC | 5 cut(s) | 1047, 1190, 1228, 1387, 1405 |
| PpsI | GAGTC | 5 cut(s) | 1047, 1190, 1228, 1387, 1405 |
| PscI | ACATGT | 1 cut(s) | 1583 |
| PshAI | GACNNNNGTC | 3 cut(s) | 2034, 2157, 2627 |
| Psp124BI | GAGCTC | 1 cut(s) | 864 |
| Psp6I | CCWGG | 1 cut(s) | 2454 |
| PspFI | CCCAGC | 1 cut(s) | 1815 |
| PspGI | CCWGG | 1 cut(s) | 2454 |
| PspPI | GGNCC | 6 cut(s) | 715, 2159, 2278, 2390, 2793, 2915 |
| PstI | CTGCAG | 2 cut(s) | 920, 2665 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1211, 1647 |
| PsuI | RGATCY | 1 cut(s) | 2451 |
| PvuII | CAGCTG | 5 cut(s) | 772, 1647, 2270, 2408, 2470 |
| RigI | GGCCGGCC | 2 cut(s) | 37, 1966 |
| RsaI | GTAC | 1 cut(s) | 905 |
| RsaNI | GTAC | 1 cut(s) | 904 |
| RseI | CAYNNNNRTG | 4 cut(s) | 1408, 1594, 2370, 2496 |
| SacI | GAGCTC | 1 cut(s) | 864 |
| SacII | CCGCGG | 1 cut(s) | 652 |
| SapI | GCTCTTC | 2 cut(s) | 1088, 1124 |
| SaqAI | TTAA | 6 cut(s) | 266, 845, 1308, 1608, 1655, 2250 |
| Sau3AI | GATC | 5 cut(s) | 967, 1776, 1802, 2451, 2554 |
| Sau96I | GGNCC | 6 cut(s) | 715, 2159, 2278, 2390, 2793, 2915 |
| SbfI | CCTGCAGG | 1 cut(s) | 2665 |
| SchI | GAGTC | 5 cut(s) | 1048, 1190, 1229, 1388, 1406 |
| ScrFI | CCNGG | 3 cut(s) | 989, 2044, 2456 |
| SdaI | CCTGCAGG | 1 cut(s) | 2665 |
| SduI | GDGCHC | 6 cut(s) | 536, 750, 864, 1591, 2517, 2583 |
| SfaNI | GCATC | 3 cut(s) | 1612, 2233, 2635 |
| SfcI | CTRYAG | 3 cut(s) | 916, 2304, 2661 |
| Sfr303I | CCGCGG | 1 cut(s) | 652 |
| SgrBI | CCGCGG | 1 cut(s) | 652 |
| SinI | GGWCC | 2 cut(s) | 715, 2159 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 1408, 1594, 2370, 2496 |
| SmlI | CTYRAG | 6 cut(s) | 863, 1215, 1633, 1862, 1994, 2594 |
| SmoI | CTYRAG | 6 cut(s) | 863, 1215, 1633, 1862, 1994, 2594 |
| SphI | GCATGC | 2 cut(s) | 1571, 2837 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 2665 |
| SspI | AATATT | 2 cut(s) | 1249, 2254 |
| SspMI | CTAG | 3 cut(s) | 1139, 1572, 1671 |
| SstI | GAGCTC | 1 cut(s) | 864 |
| StyD4I | CCNGG | 3 cut(s) | 987, 2042, 2454 |
| StyI | CCWWGG | 1 cut(s) | 1706 |
| TaaI | ACNGT | 6 cut(s) | 200, 752, 1679, 2519, 2563, 2575 |
| TaiI | ACGT | 1 cut(s) | 480 |
| TaqI | TCGA | 4 cut(s) | 679, 926, 997, 2613 |
| TatI | WGTACW | 1 cut(s) | 903 |
| TauI | GCSGC | 7 cut(s) | 8, 175, 293, 443, 446, 651, 654 |
| Tru1I | TTAA | 6 cut(s) | 266, 845, 1308, 1608, 1655, 2250 |
| Tru9I | TTAA | 6 cut(s) | 266, 845, 1308, 1608, 1655, 2250 |
| TseFI | GTSAC | 2 cut(s) | 2215, 2363 |
| Tsp45I | GTSAC | 2 cut(s) | 2215, 2363 |
| TspGWI | ACGGA | 2 cut(s) | 768, 2774 |
| VneI | GTGCAC | 3 cut(s) | 532, 1587, 2513 |
| VpaK11BI | GGWCC | 2 cut(s) | 715, 2159 |
| XapI | RAATTY | 4 cut(s) | 1301, 2057, 2501, 2603 |
| XbaI | TCTAGA | 1 cut(s) | 1670 |
| XceI | RCATGY | 4 cut(s) | 1571, 1587, 2513, 2837 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 1830, 1900, 1943 |
| XmiI | GTMKAC | 1 cut(s) | 2087 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2766 |
| XspI | CTAG | 3 cut(s) | 1139, 1572, 1671 |
| Zsp2I | ATGCAT | 1 cut(s) | 1569 |