Rh7DG359800

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000280
Physical Location & Seq
Forward (+)
47927692 .. 47932619
4928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG359800.1

Sequence Viewer

Length: 4566 bp
ATGAAATTCAAGGCGACGGCGGCGAGATTGAGGCCCGGAGGCACAAAGCGACGGAACGGCGGTGGTGTGGGTATTTTGGGAATTCAGAACGACGACGGAACAAATGGTGGAGGACCGTCTGTGGAGCCAAAGCTTGTTCCCTGGGGGAGGAAGCGGCGGAGGACAGGATGCGAGGGTATTTTGGGATATCAAAATGAAGGAAAGGCGAGGGCTGTGAAGGCGAAGAAGAAGAAGAGGAAGAAGCAGCAGCAGCAGAGAAGGGCTTCCTTGAGGGAGTTGACAATGTGCCATCAATGTAGCAAAACAGGTAAGGGAAGAACGGTGCGGTGCACATGGTGTTCGAGGAACAGGTATTGTATCCCTTGCATACAGAGGTGGTATCCCAACACATCAGAGGAAGCCATAGCTGAGGCTTGTCCTGTGTGCCGCGGCAACTGCTGCTGCTATACCTGCCTTCGATTGAATGTGCCTAATAGGTGTTTGAAGAATCTGGACCCGAAAATCGAGGAGGACACCAAGCTGGAGCACTGTAAGTATATGGTTCATCAGCTGCTTCCGTATTTGAAGATGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTCGAGGCTAATAGGCTGGGTTTACGTGATGTTTCGGAGCTCAAGGTGGACAAGGCTGATTGCGGTGAAGACGAGCGTATGTACTGCAACAACTGCAGCACTTCGATTTTTGATTTTCATAGGACTTGTCCTGAGTGTGAGTATGATCTGTGCCTCGGTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAACAAGAGAGAGGAAGTGTCCACACACATATTCTGGAGAACAAGTGAAGATGGAAGCATTCTCTGCCCCCCAACACACATGAAAGGTTGTGGTAAGCATAATTTAGAATTGAGATGCCTTTTTCCTCAGAATCATATCATGGAATTGGTAGAGAATGCAGGAAAAATAGATGCAAGTTACAATTTTTTGCCTGATCGTAGTGAAACATGTGCATACAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTTGCTTAAGTAGTAGCAGCAGCTCTAAGTTAAGGAAAGCAGCTTCTAGAGTTGCTTCCGATGATAACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAGGATAAGGATTTTGAGCATTTTCAGTGGCATTGGAAGAGGGGTGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCTGTCATGTGGCGGGCATTTAAGCAGACAAATAAAATCAACCATGCTAGTGAATCGATATTCCACGCCATTGATTGCTGGTATTGGTCTTTTATAGAAATCCACATCCTCCATTTCTGTAGTGGATATTCCAAGGGACGGTCGTATAAGACAGGATGGCCTCAGAATCTCAAGCTCACTGACTTCCCATCATCTACTGAATTTCACAATCGTCTTCCCCGGCATGGTGAGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATACTCACCCCAACAAGGGAACTCTGAACCTTGCTATCTACTTGCCACCTATCTCAGTAAAGCCGGACATGGGTCCAAAAACGTACATTGCCTATGGTTTTGCAGAGGAGCTTGGATGGGGAGATTCTGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACAACTGAAGAACTTGCAACTATAGATAAGTTGAAGAAAAAGCTGATGGAGCAAGACGAAAGGGAGATATTTGGGAATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCTCAGCAAGAGTCAGCTATAGAGAAGTTGAAGAGAAAGTATAGGGAGCAAAACGAAAGAGAGATATTTGGGAATTGTCATACAAGTGCTGGAAATGGAAGTGGGCCTCAGCAAGAGTCAGCTGAAATATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGATCCAGGATGTTCCGCAGCTGAAGAAATACATCCACAAGCACGTCAATGAATTTATGGATGTTCTCTGTAATCCTTTGCAGCATGTTACTCATGCTATACACGATCAGACTGTCTATCTGACTGTGGAACACAAGAGGAAGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTGGAGTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCAACAAACCACAGAGCCCATGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTATGAAGGAGATTCCTGGTGAGAATGTGACAAAGTGGGCATGGCCTGTGAAGGCAAAGAGGCCAAGGGTTCGCTTGGACAAGTTGAGACGTTGCCATCAGTGTCATATGAAAGTTATGGGAAGAGTGGTGCAGTGCACAAGGTGTCGGGAGAGAGGTATGGGAAGGAGGACAAGGTATTGTATCCCTTGTATACAGAGATGGTATCCCAACACATTAGAGGAAGCCATTGCTAAGGCTTGTCCTTTATGCTGCGGCAACTGCAACTGCACAGCCTGTCTTCGCTTGGATGTGCCTGTCAGGTGTTTGCAGAATCTAGAGCCAAAAATCGACAAGGGTACCAAGCTGGAGCACTGTAAGTATTTGGTTCATCAGCTGCTTCCCTATTTGAAGATGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTGGAAGCCAAGAGGCAGGGCTTACGTGACCTTTCGGAGCTCAAGGTGGAGAAGGCAAAAAGCGGTGATGGAGTGCGTATGTACTGCAACAGCTGCAGCACTTCGGTTTTTGATTTTCATAGAACTTGTCCCGGATGTGAGTATGATCTGTGCCTCGGTTGCTGCCGGGAGATTCGAGATGGCAAGTGGAAGAAGAGAGCTCAAGCGTCCACATTTGAGTGGAAATGGAGTGCAAGTGAAGATGGAAGCATTCTCTGCCCCTCAACACACATGAAAGGTTGTGGTAAGCACAATTTAGAATTGAGATGCATTTTTTCTCAGAATCATGTCATGGAATTGGTAGAGAAAGCAGGAGAAATAGATGCAAGTTACAATTTTTTGCCTGATCGTAGTGAAACATGTGCATATAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTTAAGTAGTAGCAGCTGCTGTAAGTTAAGGAAAGCAGCTTCTAGAGTTGCTTCTGATAGTAACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAAGCTAAGGATTTTGAGCATTTTCAGTGGCATTGGAAGAGGGGTGAGCCTGTGATCGTTAGCAATGCGCTTGAAAATGGATCAGGTTTAAGCTGGGAACCATATGTCATGTGGCAGGCATTTAGAAAGACAAATAATATCAACCATGCTAGTGAATCGACAGTCCATGCCATTGATTGCTGGGATTGGTGTTTTATAGAAATAAACATCCACCATTTCTTTTGTGGATATTCCAAAGGACGGTCTTATAAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATCATCTACTGAGTTTCACAATCGTCTTCCCCGGCATGGTAGGGAATTTTTATGTTGTCTGCCTTTCAAGGAATATACTCACCCCAACAAGGGAACTCTGAACCTTGCTATCGACTTGCCACCTATCTCAGTAAAGCCAGACATGGGTCCAAAAGCATATATTGCCTATGGTTTTGCACAAGAGCTTGGACGGGGAGATTCTGTGACCAAGCTTCACTGCAATATGTCTGATGCGGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACAACTGGGCAACTTGTAACTGTAGAGAAGTTGAAGAAAAAGCACAGGGAGCTAGACAAAAGAGAGATATTTGGGAATTGTCACTCAAGTGCTGGAAATGGAAGTGGGTCTCAGGAAGAATTAGCTGATGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGACTCAGGATGTTCCGCAGCTGGAGAAATACATCCACAATCACGTCAATGAATTTAAGCATGTGTTCTGTAATCCTTTGCAGCATGTTACTCATGCTATACATGATCAGACTGTTTATCTCACTGCGGAGCACAAGAGGAAGCTAAAGGAGGAATTCGGTATCGAGCCTTGGACATTTGTCCAAAAGCTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTGTCAAAGTTGCACTGGACTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACATACCACAGAGCCCATGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTGTGAAGGAGATTTCTGGTGAGAATGTGATGACAGAGGATGGGAAGTCTAGGGGAGGCGCAAGGGAATTAAGGCAAGGTGTAAGGCGCAAGTCTTACAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1521

Amino Acids

173.56

Weight (kDa)

8.57

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjC PF02373 653 - 747 8.4e-13 JmjC domain, hydroxylase
JmjC PF02373 1348 - 1444 6.5e-13 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3582
AarI CACCTGC 2 cut(s) 574, 2767
Acc36I ACCTGC 5 cut(s) 458, 574, 2174, 2767, 4265
Acc65I GGTACC 1 cut(s) 2705
AccB1I GGYRCC 3 cut(s) 1949, 2705, 4040
AccB7I CCANNNNNTGG 2 cut(s) 1127, 3323
AccI GTMKAC 2 cut(s) 1502, 2560
AccII CGCG 1 cut(s) 429
AccIII TCCGGA 2 cut(s) 1968, 4059
AclWI GGATC 4 cut(s) 1225, 1966, 1979, 3421
AcsI RAATTY 9 cut(s) 5, 81, 1439, 1472, 2021, 2123, 3668, 4112, 4214
AcuI CTGAAG 4 cut(s) 1707, 1725, 2012, 3903
AdeI CACNNNGTG 2 cut(s) 336, 2511
AfaI GTAC 4 cut(s) 683, 1586, 2707, 2876
AflII CTTAAG 2 cut(s) 1045, 3241
AflIII ACRYGT 2 cut(s) 995, 3191
AjiI CACGTC 2 cut(s) 2014, 4105
AjnI CCWGG 4 cut(s) 140, 1137, 1974, 2383
AleI CACNNNNGTG 2 cut(s) 1785, 3981
Alw21I GWGCWC 8 cut(s) 332, 528, 642, 2507, 2721, 2835, 2995, 4194
Alw26I GTCTC 2 cut(s) 2449, 4008
Alw44I GTGCAC 2 cut(s) 328, 2503
AlwI GGATC 4 cut(s) 1225, 1966, 1979, 3421
AlwNI CAGNNNCTG 1 cut(s) 3258
Aor13HI TCCGGA 2 cut(s) 1968, 4059
AoxI GGCC 9 cut(s) 32, 1397, 1692, 1805, 1910, 2411, 2429, 3593, 3888
ApaLI GTGCAC 2 cut(s) 328, 2503
ApoI RAATTY 9 cut(s) 5, 81, 1439, 1472, 2021, 2123, 3668, 4112, 4214
Asp700I GAANNNNTTC 3 cut(s) 262, 2286, 4377
Asp718I GGTACC 1 cut(s) 2705
AspLEI GCGC 3 cut(s) 3403, 4520, 4548
AspS9I GGNCC 9 cut(s) 33, 113, 493, 1574, 1693, 1805, 1910, 3770, 3889
AsuC2I CCSGG 6 cut(s) 36, 767, 1459, 2925, 2960, 3655
AvaII GGWCC 4 cut(s) 113, 493, 1574, 3770
BaeGI GKGCMC 2 cut(s) 332, 2507
BaeI ACNNNNGTAYC 2 cut(s) 2689, 2722
BamHI GGATCC 1 cut(s) 1971
BanI GGYRCC 3 cut(s) 1949, 2705, 4040
BanII GRGCYC 5 cut(s) 642, 2317, 2835, 2995, 4408
BarI GAAGNNNNNNTAC 2 cut(s) 1623, 1655
BbsI GAAGAC 4 cut(s) 675, 1445, 2639, 3641
Bbv12I GWGCWC 8 cut(s) 332, 528, 642, 2507, 2721, 2835, 2995, 4194
BbvCI CCTCAGC 3 cut(s) 408, 1809, 1914
BceAI ACGGC 5 cut(s) 33, 73, 1022, 2122, 3218
BciT130I CCWGG 4 cut(s) 142, 1139, 1976, 2385
BciVI GTATCC 4 cut(s) 368, 390, 2561, 2583
BclI TGATCA 1 cut(s) 4165
BcnI CCSGG 6 cut(s) 36, 767, 1459, 2925, 2960, 3655
BcoDI GTCTC 2 cut(s) 2449, 4008
BfaI CTAG 7 cut(s) 1086, 1287, 2684, 3282, 3483, 3947, 4509
BfmI CTRYAG 8 cut(s) 694, 1357, 1719, 1824, 2181, 2887, 3915, 4272
BfrI CTTAAG 2 cut(s) 1045, 3241
BfuAI ACCTGC 5 cut(s) 458, 574, 2174, 2767, 4265
BfuI GTATCC 4 cut(s) 368, 390, 2561, 2583
Bme18I GGWCC 4 cut(s) 113, 493, 1574, 3770
BmgBI CACGTC 2 cut(s) 2014, 4105
BmgT120I GGNCC 9 cut(s) 33, 113, 493, 1574, 1693, 1805, 1910, 3770, 3889
BmrI ACTGGG 1 cut(s) 3909
BmuI ACTGGG 1 cut(s) 3909
BoxI GACNNNNGTC 4 cut(s) 1572, 2147, 3768, 4238
BpiI GAAGAC 4 cut(s) 675, 1445, 2639, 3641
BpmI CTGGAG 7 cut(s) 542, 844, 2255, 2351, 2735, 4103, 4442
Bpu10I CCTNAGC 5 cut(s) 408, 1809, 1914, 2601, 3339
BpuEI CTTGAG 8 cut(s) 289, 626, 1394, 2099, 2819, 2979, 3590, 3964
BpuMI CCSGG 6 cut(s) 36, 767, 1459, 2925, 2960, 3655
Bsa29I ATCGAT 1 cut(s) 1295
BsaAI YACGTR 2 cut(s) 626, 2819
BsaI GGTCTC 1 cut(s) 4008
BsaWI WCCGGW 2 cut(s) 1968, 4059
Bse1I ACTGG 3 cut(s) 2238, 3904, 4329
Bse3DI GCAATG 4 cut(s) 1207, 1587, 2595, 3403
BseAI TCCGGA 2 cut(s) 1968, 4059
BseBI CCWGG 4 cut(s) 142, 1139, 1976, 2385
BseCI ATCGAT 1 cut(s) 1295
BseMI GCAATG 4 cut(s) 1207, 1587, 2595, 3403
BseNI ACTGG 3 cut(s) 2238, 3904, 4329
BseRI GAGGAG 2 cut(s) 521, 1622
BseSI GKGCMC 2 cut(s) 332, 2507
BseYI CCCAGC 4 cut(s) 616, 1230, 3426, 3513
BsgI GTGCAG 2 cut(s) 2519, 2620
Bsh1236I CGCG 1 cut(s) 429
Bsh1285I CGRYCG 1 cut(s) 1382
BshFI GGCC 9 cut(s) 34, 1399, 1694, 1807, 1912, 2413, 2431, 3595, 3890
BshNI GGYRCC 3 cut(s) 1949, 2705, 4040
BshVI ATCGAT 1 cut(s) 1295
BsiEI CGRYCG 1 cut(s) 1382
BsiHKAI GWGCWC 8 cut(s) 332, 528, 642, 2507, 2721, 2835, 2995, 4194
BsiSI CCGG 9 cut(s) 36, 766, 1459, 1565, 1969, 2925, 2959, 3655, 4060
BslFI GGGAC 4 cut(s) 1389, 1973, 2907, 4064
BsmAI GTCTC 2 cut(s) 2449, 4008
BsmBI CGTCTC 1 cut(s) 2449
BsmFI GGGAC 4 cut(s) 1389, 1973, 2907, 4064
BsmI GAATGC 3 cut(s) 846, 949, 3042
BsnI GGCC 9 cut(s) 34, 1399, 1694, 1807, 1912, 2413, 2431, 3595, 3890
Bso31I GGTCTC 1 cut(s) 4008
Bsp13I TCCGGA 2 cut(s) 1968, 4059
BspANI GGCC 9 cut(s) 34, 1399, 1694, 1807, 1912, 2413, 2431, 3595, 3890
BspDI ATCGAT 1 cut(s) 1295
BspEI TCCGGA 2 cut(s) 1968, 4059
BspFNI CGCG 1 cut(s) 429
BspMAI CTGCAG 4 cut(s) 698, 2185, 2891, 4276
BspMI ACCTGC 5 cut(s) 458, 574, 2174, 2767, 4265
BspPI GGATC 4 cut(s) 1225, 1966, 1979, 3421
BspT107I GGYRCC 3 cut(s) 1949, 2705, 4040
BspTI CTTAAG 2 cut(s) 1045, 3241
BspTNI GGTCTC 1 cut(s) 4008
BsrDI GCAATG 4 cut(s) 1207, 1587, 2595, 3403
BsrI ACTGG 3 cut(s) 2238, 3904, 4329
BssNAI GTATAC 2 cut(s) 1503, 2561
BssT1I CCWWGG 5 cut(s) 1121, 1371, 2432, 3317, 4229
Bst1107I GTATAC 2 cut(s) 1503, 2561
Bst2UI CCWGG 4 cut(s) 142, 1139, 1976, 2385
Bst6I CTCTTC 8 cut(s) 227, 1169, 1832, 2277, 2485, 2981, 3365, 4368
BstAFI CTTAAG 2 cut(s) 1045, 3241
BstAPI GCANNNNNTGC 6 cut(s) 438, 693, 852, 2886, 3048, 3785
BstBAI YACGTR 2 cut(s) 626, 2819
BstC8I GCNNGC 4 cut(s) 1254, 2355, 3450, 4446
BstDSI CCRYGG 2 cut(s) 427, 2138
BstENI CCTNNNNNAGG 1 cut(s) 145
BstFNI CGCG 1 cut(s) 429
BstHHI GCGC 3 cut(s) 3403, 4520, 4548
BstMAI GTCTC 2 cut(s) 2449, 4008
BstMCI CGRYCG 1 cut(s) 1382
BstNI CCWGG 4 cut(s) 142, 1139, 1976, 2385
BstNSI RCATGY 7 cut(s) 999, 2057, 2357, 3195, 4124, 4148, 4448
BstPAI GACNNNNGTC 4 cut(s) 1572, 2147, 3768, 4238
BstSFI CTRYAG 8 cut(s) 694, 1357, 1719, 1824, 2181, 2887, 3915, 4272
BstSLI GKGCMC 2 cut(s) 332, 2507
BstUI CGCG 1 cut(s) 429
BstV2I GAAGAC 4 cut(s) 675, 1445, 2639, 3641
BstX2I RGATCY 1 cut(s) 1971
BstXI CCANNNNNNTGG 2 cut(s) 2159, 4250
BstYI RGATCY 1 cut(s) 1971
BstZ17I GTATAC 2 cut(s) 1503, 2561
Bsu15I ATCGAT 1 cut(s) 1295
BsuI GTATCC 4 cut(s) 368, 390, 2561, 2583
BsuRI GGCC 9 cut(s) 34, 1399, 1694, 1807, 1912, 2413, 2431, 3595, 3890
BsuTUI ATCGAT 1 cut(s) 1295
BtgI CCRYGG 2 cut(s) 427, 2138
BtrI CACGTC 2 cut(s) 2014, 4105
BtsI GCAGTG 4 cut(s) 1642, 2507, 3838, 4182
BveI ACCTGC 5 cut(s) 458, 574, 2174, 2767, 4265
Cac8I GCNNGC 4 cut(s) 1254, 2355, 3450, 4446
CaiI CAGNNNCTG 1 cut(s) 3258
CfoI GCGC 3 cut(s) 3403, 4520, 4548
Cfr13I GGNCC 9 cut(s) 33, 113, 493, 1574, 1693, 1805, 1910, 3770, 3889
Cfr42I CCGCGG 1 cut(s) 430
ClaI ATCGAT 1 cut(s) 1295
CseI GACGC 1 cut(s) 2988
Csp6I GTAC 4 cut(s) 682, 1585, 2706, 2875
CviQI GTAC 4 cut(s) 682, 1585, 2706, 2875
DraIII CACNNNGTG 2 cut(s) 336, 2511
Eam1104I CTCTTC 8 cut(s) 227, 1169, 1832, 2277, 2485, 2981, 3365, 4368
EarI CTCTTC 8 cut(s) 227, 1169, 1832, 2277, 2485, 2981, 3365, 4368
EciI GGCGGA 1 cut(s) 172
Ecl136II GAGCTC 3 cut(s) 640, 2833, 2993
Eco130I CCWWGG 5 cut(s) 1121, 1371, 2432, 3317, 4229
Eco24I GRGCYC 5 cut(s) 642, 2317, 2835, 2995, 4408
Eco31I GGTCTC 1 cut(s) 4008
Eco32I GATATC 1 cut(s) 188
Eco47I GGWCC 4 cut(s) 113, 493, 1574, 3770
Eco53kI GAGCTC 3 cut(s) 640, 2833, 2993
Eco57I CTGAAG 4 cut(s) 1707, 1725, 2012, 3903
EcoICRI GAGCTC 3 cut(s) 640, 2833, 2993
EcoNI CCTNNNNNAGG 1 cut(s) 145
EcoRI GAATTC 3 cut(s) 81, 2123, 4214
EcoRII CCWGG 4 cut(s) 140, 1137, 1974, 2383
EcoRV GATATC 1 cut(s) 188
EcoT14I CCWWGG 5 cut(s) 1121, 1371, 2432, 3317, 4229
EcoT22I ATGCAT 1 cut(s) 3104
EcoT38I GRGCYC 5 cut(s) 642, 2317, 2835, 2995, 4408
ErhI CCWWGG 5 cut(s) 1121, 1371, 2432, 3317, 4229
Esp3I CGTCTC 1 cut(s) 2449
FalI AAGNNNNNCTT 4 cut(s) 1680, 1712, 3876, 3908
FaqI GGGAC 4 cut(s) 1389, 1973, 2907, 4064
FauI CCCGC 1 cut(s) 1245
FauNDI CATATG 2 cut(s) 2475, 3436
FbaI TGATCA 1 cut(s) 4165
FblI GTMKAC 2 cut(s) 1502, 2560
FriOI GRGCYC 5 cut(s) 642, 2317, 2835, 2995, 4408
FspBI CTAG 7 cut(s) 1086, 1287, 2684, 3282, 3483, 3947, 4509
GlaI GCGC 3 cut(s) 3402, 4519, 4547
GsaI CCCAGC 4 cut(s) 620, 1234, 3430, 3517
GsuI CTGGAG 7 cut(s) 542, 844, 2255, 2351, 2735, 4103, 4442
HaeIII GGCC 9 cut(s) 34, 1399, 1694, 1807, 1912, 2413, 2431, 3595, 3890
HapII CCGG 9 cut(s) 36, 766, 1459, 1565, 1969, 2925, 2959, 3655, 4060
HgaI GACGC 1 cut(s) 2988
HhaI GCGC 3 cut(s) 3403, 4520, 4548
Hin6I GCGC 3 cut(s) 3401, 4518, 4546
HinP1I GCGC 3 cut(s) 3401, 4518, 4546
HincII GTYRAC 1 cut(s) 279
HindII GTYRAC 1 cut(s) 279
HindIII AAGCTT 4 cut(s) 131, 1637, 3833, 4247
HpaII CCGG 9 cut(s) 36, 766, 1459, 1565, 1969, 2925, 2959, 3655, 4060
Hpy99I CGWCG 4 cut(s) 19, 54, 95, 98
HpyAV CCTTC 9 cut(s) 191, 211, 252, 464, 2368, 2413, 2526, 2839, 4459
HpyCH4IV ACGT 6 cut(s) 625, 1583, 2013, 2458, 2818, 4104
HpySE526I ACGT 6 cut(s) 625, 1583, 2013, 2458, 2818, 4104
HspAI GCGC 3 cut(s) 3401, 4518, 4546
Kpn2I TCCGGA 2 cut(s) 1968, 4059
KpnI GGTACC 1 cut(s) 2709
Ksp22I TGATCA 1 cut(s) 4165
KspI CCGCGG 1 cut(s) 430
MaeI CTAG 7 cut(s) 1086, 1287, 2684, 3282, 3483, 3947, 4509
MaeII ACGT 6 cut(s) 625, 1583, 2013, 2458, 2818, 4104
MflI RGATCY 1 cut(s) 1971
MlyI GAGTC 3 cut(s) 1826, 1931, 4057
MmeI TCCRAC 4 cut(s) 1108, 2324, 3304, 4415
Mph1103I ATGCAT 1 cut(s) 3104
MroI TCCGGA 2 cut(s) 1968, 4059
MroXI GAANNNNTTC 3 cut(s) 262, 2286, 4377
MspCI CTTAAG 2 cut(s) 1045, 3241
MspI CCGG 9 cut(s) 36, 766, 1459, 1565, 1969, 2925, 2959, 3655, 4060
Mva1269I GAATGC 3 cut(s) 846, 949, 3042
MvaI CCWGG 4 cut(s) 142, 1139, 1976, 2385
MvnI CGCG 1 cut(s) 429
NciI CCSGG 6 cut(s) 36, 767, 1459, 2925, 2960, 3655
NdeI CATATG 2 cut(s) 2475, 3436
NmuCI GTSAC 5 cut(s) 1778, 2395, 2819, 3826, 3974
NsiI ATGCAT 1 cut(s) 3104
NspI RCATGY 7 cut(s) 999, 2057, 2357, 3195, 4124, 4148, 4448
OliI CACNNNNGTG 2 cut(s) 1785, 3981
PaeI GCATGC 2 cut(s) 2357, 4448
PaqCI CACCTGC 2 cut(s) 574, 2767
PasI CCCWGGG 1 cut(s) 141
PciI ACATGT 2 cut(s) 995, 3191
PctI GAATGC 3 cut(s) 846, 949, 3042
PdmI GAANNNNTTC 3 cut(s) 262, 2286, 4377
PflMI CCANNNNNTGG 2 cut(s) 1127, 3323
PfoI TCCNGGA 3 cut(s) 1137, 1974, 2923
PleI GAGTC 3 cut(s) 1825, 1930, 4057
PpsI GAGTC 3 cut(s) 1825, 1930, 4057
Ppu21I YACGTR 2 cut(s) 626, 2819
PscI ACATGT 2 cut(s) 995, 3191
PshAI GACNNNNGTC 4 cut(s) 1572, 2147, 3768, 4238
PsiI TTATAA 1 cut(s) 3582
Psp124BI GAGCTC 3 cut(s) 642, 2835, 2995
Psp6I CCWGG 4 cut(s) 140, 1137, 1974, 2383
PspFI CCCAGC 4 cut(s) 616, 1230, 3426, 3513
PspGI CCWGG 4 cut(s) 140, 1137, 1974, 2383
PspPI GGNCC 9 cut(s) 33, 113, 493, 1574, 1693, 1805, 1910, 3770, 3889
PstI CTGCAG 4 cut(s) 698, 2185, 2891, 4276
PstNI CAGNNNCTG 1 cut(s) 3258
PsuI RGATCY 1 cut(s) 1971
PvuII CAGCTG 9 cut(s) 550, 1685, 1928, 1990, 2743, 2886, 3255, 3881, 4081
RsaI GTAC 4 cut(s) 683, 1586, 2707, 2876
RsaNI GTAC 4 cut(s) 682, 1585, 2706, 2875
SacI GAGCTC 3 cut(s) 642, 2835, 2995
SacII CCGCGG 1 cut(s) 430
Sau96I GGNCC 9 cut(s) 33, 113, 493, 1574, 1693, 1805, 1910, 3770, 3889
SbfI CCTGCAGG 2 cut(s) 2185, 4276
SchI GAGTC 3 cut(s) 1826, 1931, 4057
SdaI CCTGCAGG 2 cut(s) 2185, 4276
SfcI CTRYAG 8 cut(s) 694, 1357, 1719, 1824, 2181, 2887, 3915, 4272
Sfr303I CCGCGG 1 cut(s) 430
SgrBI CCGCGG 1 cut(s) 430
SinI GGWCC 4 cut(s) 113, 493, 1574, 3770
SphI GCATGC 2 cut(s) 2357, 4448
Sse8387I CCTGCAGG 2 cut(s) 2185, 4276
SspI AATATT 2 cut(s) 1669, 3865
SspMI CTAG 7 cut(s) 1086, 1287, 2684, 3282, 3483, 3947, 4509
SstI GAGCTC 3 cut(s) 642, 2835, 2995
StyI CCWWGG 5 cut(s) 1121, 1371, 2432, 3317, 4229
TaiI ACGT 6 cut(s) 628, 1586, 2016, 2461, 2821, 4107
TatI WGTACW 2 cut(s) 681, 2874
TauI GCSGC 5 cut(s) 23, 157, 429, 432, 2625
TseFI GTSAC 5 cut(s) 1778, 2395, 2819, 3826, 3974
Tsp45I GTSAC 5 cut(s) 1778, 2395, 2819, 3826, 3974
TspGWI ACGGA 5 cut(s) 67, 111, 546, 2294, 4385
Van91I CCANNNNNTGG 2 cut(s) 1127, 3323
Vha464I CTTAAG 2 cut(s) 1045, 3241
VneI GTGCAC 2 cut(s) 328, 2503
VpaK11BI GGWCC 4 cut(s) 113, 493, 1574, 3770
XagI CCTNNNNNAGG 1 cut(s) 145
XapI RAATTY 9 cut(s) 5, 81, 1439, 1472, 2021, 2123, 3668, 4112, 4214
XbaI TCTAGA 3 cut(s) 1085, 2683, 3281
XceI RCATGY 7 cut(s) 999, 2057, 2357, 3195, 4124, 4148, 4448
XcmI CCANNNNNNNNNTGG 4 cut(s) 1245, 1358, 3441, 3554
XmiI GTMKAC 2 cut(s) 1502, 2560
XmnI GAANNNNTTC 3 cut(s) 262, 2286, 4377
XspI CTAG 7 cut(s) 1086, 1287, 2684, 3282, 3483, 3947, 4509
Zsp2I ATGCAT 1 cut(s) 3104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.