Rh7BG355400

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000280
Physical Location & Seq
Forward (+)
37237013 .. 37241623
4611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG355400.1

Sequence Viewer

Length: 4611 bp
ATGGAAATCCAGGCCACGGCGGCGAGATTGAGGCCCCGAGGCACAAAGCGGCCGAACGGCGGTGGTGTGGTGGAGAAGCAGGCCGAGGGTATTTCGGGAATTCAGAACGACGACGGAACAATTGGGGAAGACGTGGAGCCAGCAGCGCCAAAGGTTGTTCCCTGGGGGAGGAAGCGGCGGAGGACAGGATGCGAGGGTATTTTGGGAGATCAAAATGAAGGAGAGGCAAGGGCGGCAAATTGGTGCACAAGGGCTCACTTGGACGTGGGGAAATGTTGCCATCAATGTAAGCGAAATTGTAAGCGAAGAGTGGTGCGGTGCACAAGGTGTCCCGAAAGAGGTAAGCGAAATAGGTATTGTCTCCCTTGCATACGGAGATGGTATCCCAACACCTCACAGGAAGCCATTGCTGAGGCTTGTCCTGTGTGCCGCGGCAACTGCAACTGCACAGCCTGCCTTCGATTGGATGCGCGTTTCGAGAATCGAGACCCGAAAATCGATGAGGAGACCAAGCTGGAGCACTCTAAGTATTTGGTTCATCAGCTGCTTCCATATTTGAAGAGGATTAGGGATGAGCAGGTGAGTGAGATGGTGGTGGAAGCCAAGAGGCAGGGGTTACGTGACCTTTCGGAGCTCAAGGTGGACAAGGCTAATTGCGGCGGTGACGGAGTGCGTATGTACTGCAACAGCTGCAGCACTTCGATTTTTGATTTTCATAGGACTTGTCCCGGGTGTGAGTATGATCTGTGCCTCGGCTGCTGCCGGGAGATTCGAGATGGCAAGTGGAACAAGAGAGCTCAAGCGTCCACATTTGAGTGGAAATGGAGAGCAAGTGAAGATGGAAGCATTCTCTGCCCCCCAACACACATGAAAGGGTGTGGTAAGCACAATTTAGAATTGAGATGCATTTTTTCTCAGAATCATGTCATGGAATTGGTAGAGAAAGCAGGAGAAATAGATGCAAGTTACAATTTTTTGCCTGATCGTAGTGAAACATGTGCATATAAGTGTTCATGTGTTAAACCTGTGGATGATGCCGTTAGCTCAAGTAGCAGCAGCTGTAAGTTAAGGAAAGCAGCTTCTAGAGTTGCTTCTGATAGTGACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAAGATAAGGATTTTGGGCATTTTCAGTGGCATTGGAAGAGGGGCGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCTGTCATGTGGCGGGCATTTAGACAGATAGAGAAAGTCAAGAATGATAGTGAATTGACATTCCACGCCATTGATTGCTGGGATTGGTGTTTTATAGAAATCAACATCCTCCATTTCTTTAGTGGATATTCCAACGGACGGTCTTATAAGACAGGATGGCCTCAGAATCTCAAGCTCATTGACTTCCCATCATCTGCTGAGTTTTACAAACGTCTTCCCCGGCATTGTAAGGAATTTTTATGTTGTCTGCCTCTCAAGGAGTATACTCACCCCAACAAGGGAACTCTGAACCTTGCTATCTACTTGCCACCTACCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACAGGGAGATTCCGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACAACTGAACAACTTGCAACTATAGAGAAGTTGAAGAAAAAGCACAGGGAGCAAAACGAAAGAGAGATATTTGGGGATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCTCAGCAAGAGTCAGCTATAGAGAAGTTGAAGAGAAAGCACAGGGAGCAAAACGAAAGAGAGATATTTGGGAATTGTCACACAAGTGCTGGAAATGGAAGTGGGCCTCAGCAAGAGTCAGCTGAAGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGATCCAGGATGTTCCGCAGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTATGCATGTACTTGGTAATCCTTTGCAGCATGTTACTCATGCTATACATGATCAGACTGTCTATCTCACTGTGGAACACAAGAGGAAGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAAATTGAAGTCTTGTATCAAAGTTGCACTGGAGTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACAAACCACAGCGCCAGTGAGGACAAGCTGGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTATTAAGGAGATTTCTGGTGAGAATGTGAAGGTAAAGAGGGCAAGGGATGTGAAGGCAAAGAGGGCAAGGGATGTGAAGGCAAAGAGGGCAAGGGCTCTGAAGGCAAAGAGGGCAGGGGCTCCCTTGGACGTGTCGACAATTTGCCATCAGTGTCAGAGAAATGTTAAGGGAAGAGTGGTGTGGTGCACAAGGTGTGCGAGGAAGAGGTATTGTATTCCTTGCATACAGAGATGGTATCCCAACACATCAGAGGAAGCCATTGCTAAGGCTTGTCCTGTGTGCCGCGGCAACTGCAACTGCACAGCCTGCCTTCAATTGGATGTGCCTGTCAGGCGTTTGCAGAATCTAGACCCGAAAATCGACAAGGGTACCAAGCTGGAGCACTGTAAGTACTTGGTTCATCAGCTGCTTCCCTATTTGAAAAGGATTAGGGATGAGCAGGTGATTGAGATGCTGATTGAGGCTAAGAGGCAGGGATTACGTGATGTTTCGGAGCTCAAGGTGGAGAAGGCTAATTGCGGTGAAGATGAGCGTATGTACTGCAACAACTGCAGCACGTCGATTTTTGATTTTCATAGGAGTTGTCCTGAGTGTGAGTATGATCTGTGCCTCAGTTGCTGCCGGGAGATTCGAGATGGCAGGTGGAAGAAGAGAGGGATGGAGGAGAAAGCTCAAGTGGAGAAAGCTCAAGTGTCCACATTTGAGTGGAGAGCAAGTGAAGATGGAAGCATTCTCTGGACACATATGAAATGCTGTGGTAAGCATAAATTAGAATTGAAATGCAAATTTTCTCAGAATCATGTCATAGAATTGGTAGAGAAAGCAGGAAAAATAGATGAAAGTTACAGTCATTTGCGTGCTAGTAGTGAAACACCTGCACACAAGTGTTCATGTGGTAAACCTGTGGATGATGCCGTTAGCTCAAGTAGCAGCTGCTCTAAGTTAAGGAAAGCAGCTTCTAGAGATGGTTCTGATGATAACTACTTGTTCTGTCCAAGGGTTGGACAAATCCAAGATAAGGATTTTGGGCATTTTCAGTGGCATTGGATGAGGGGTGAGCCTGTGATCGTTAGCAATGCACTTGAAAATGGATCAGGTTTAAGCTGGGAACCATCTGTCATGTGGCGGGCATTTAGAAAGATAAAGAAGATCAAGCATGATAGTGAATTGACAGTCCACGCCATTGATTGCTGGGATTGGTGTTTTATAGAAATCAACATACTCCATTTCTTTAGTGGATATTCTAACGGACGGTCTTATAAGACAGGATGGCCTCAGAATCTCAAGCTCAATGACTTCCCATCATCTACCGAGTTTCACAATCGGCTTCCCCGGCATGTTAAGGAATTTTTATGTTGTCTGCCTTTCAAGGAGTATACTCACCCCAACAAGGGAAATCTGAACCTTGCTATCTACTTGCCACCTACCTCAGTAAAGCCGGACATGGGTCCAAAAACATATATTGCCTATGGTTTTGCACAGGAGCTTGGACGGGGAGATTCTGTAACCAAGCTTCACTGCAATATGTCTGATGCGGTTAATATTCTGACACATACAGCTGAAGTGGCCCTTACAACTGAACAACTGGCAACTATAGAGAAGTTGAATAAAAAGCACAGGGAGCAAGACGAAAGAGAGATATTTGGGAATTGTCACACAATTGCTGGAAATGGAAGTGGGCCTCAGCAAGAATCAGCTGAAGTATGTGAGGATGGAGGTGCCTTATGGGACATTTTCCGGATTCAGGATGTTCCGCTGCTGGAGAAATACATCCACAAGCACGTCAATGAATTTATGCATGTGCTCTGTAATCCTTTGCAGCGTGTTACTCATGCTATACATGATCAGACTGTTTATCTCACTGTGGAGCACAAGAGGAAGCTCAAGGAGGAATTCGGTATCGAGCCGTGGACATTTGTCCAAAAACTTGGAGATGCTGTTTTCATTCCTGCAGGTTGTCCTCATCAGGTGAGGAACTTGAAGTCTTGTATCAAAGTTGCACTGGAGTTTGTTTCCCCTGAGAATGTGGGTGAGTGCTTTCGTTTGACGGAAGAGTTTCGCACACTTCCGACAAACCACAGAGCCAGTGAGGACAAGCTAGAGGTGAAGAAAATGATTGTGCATGCTGTTTGTGAAGCTGTGAAGGAGATTTCTGCTGAGAATGTGATGACTGAGGTTGTGAAGCCTAGGGGAGGCGCAAGGGAATTAAGGCAAGGTGTAAGGCTCAAGTCTTACAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1536

Amino Acids

174.94

Weight (kDa)

8.57

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 88 - 156 3e-06 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 652 - 746 1.3e-11 JmjC domain, hydroxylase
zf-4CXXC_R1 PF10497 834 - 894 9.9e-07 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 1360 - 1459 5.6e-13 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07610 AT3G07610 AT3G07610
fragaria_vesca FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24540 FvH4_5g24640 FvH4_5g29990 FvH4_5g29990 FvH4_5g29990 FvH4_7g06860 FvH4_7g09570 FvH4_7g09571 FvH4_7g09571
malus_domestica MD05G1184100.v1.1 MD06G1012500.v1.1 MD07G1099600.v1.1 MD07G1099700.v1.1
prunus_persica Prupe.2G123400_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1 Prupe.5G015100_v2.0.a1
pyrus_communis pycom06g01040 pycom10g12640
rosa_chinensis RchiOBHm_Chr1g0346431 RchiOBHm_Chr1g0346441 RchiOBHm_Chr3g0461961 RchiOBHm_Chr3g0461971 RchiOBHm_Chr3g0461991 RchiOBHm_Chr3g0462031 RchiOBHm_Chr3g0462041 RchiOBHm_Chr7g0215021 RchiOBHm_Chr7g0223871 RchiOBHm_Chr7g0223931 RchiOBHm_Chr7g0223991 RchiOBHm_Chr7g0224011 RchiOBHm_Chr7g0224041
rosa_laevigata RLG00000001919 RLG00000001920 RLG00000001924 RLG00000002699 RLG00000024884 RLG00000024888 RLG00000024889 RLG00000028799 RLG00000028800
rosa_multiflora Rmu_co8191480.1_g000001 Rmu_co8411417.1_g000001 Rmu_co8440445.1_g000001 Rmu_co8460737.1_g000001 Rmu_sc0003363.1_g000077 Rmu_sc0004308.1_g000026 Rmu_sc0004540.1_g000014 Rmu_sc0004540.1_g000026 Rmu_sc0007736.1_g000006 Rmu_sc0007736.1_g000008 Rmu_sc0007777.1_g000006 Rmu_sc0007777.1_g000009 Rmu_sc0007777.1_g000029 Rmu_sc0008432.1_g000002 Rmu_sc0008835.1_g000002 Rmu_sc0029902.1_g000002
rosa_roxburghii Rroxscaffold_3G00235160 Rroxscaffold_3G00235180 Rroxscaffold_3G00235210 Rroxscaffold_3G00244660 Rroxscaffold_4G00308520 Rroxscaffold_4G00308530 Rroxscaffold_6G00417830 Rroxscaffold_6G00417840
rosa_rugosa Rorug01G0179700 Rorug01G0179800 Rorug07G0149600 Rorug07G0221000 Rorug07G0221100 Rorug07G0221100
rosa_samantha Rh1AG199200 Rh1AG199300 Rh1AG199400 Rh1BG165100 Rh1BG165200 Rh1BG165300 Rh1BG165400 Rh1CG184300 Rh1CG184400 Rh1DG195800 Rh1DG195900 Rh1DG196000 Rh2BG420000 Rh3AG112800 Rh3BG115600 Rh3BG115700 Rh3BG115800 Rh3BG115900 Rh3CG117900 Rh3CG118300 Rh3CG118400 Rh3CG118500 Rh3DG117400 Rh3DG117500 Rh3DG117600 Rh6AG306600 Rh6BG312200 Rh7BG278000 Rh7BG355000 Rh7BG355400 Rh7BG355800 Rh7BG355900 Rh7BG356400 Rh7CG305900 Rh7CG382300 Rh7CG382800 Rh7CG383400 Rh7CG383500 Rh7DG290000 Rh7DG358300 Rh7DG358500 Rh7DG359600 Rh7DG359800
rosa_wichuraiana Rw0G007860 Rw1G016720 Rw1G016730 Rw7G024430 Rw7G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1383, 3627
AarI CACCTGC 4 cut(s) 568, 2797, 2997, 3250
Acc36I ACCTGC 6 cut(s) 568, 2171, 2797, 2997, 3250, 4310
Acc65I GGTACC 1 cut(s) 2735
AccB1I GGYRCC 3 cut(s) 1946, 2735, 4085
AccB7I CCANNNNNTGG 2 cut(s) 1124, 3368
AccI GTMKAC 3 cut(s) 1499, 2501, 3743
AccII CGCG 3 cut(s) 432, 472, 2652
AccIII TCCGGA 2 cut(s) 1965, 4104
AclWI GGATC 4 cut(s) 1222, 1963, 1976, 3466
AcoI YGGCCR 1 cut(s) 50
AcsI RAATTY 8 cut(s) 99, 1469, 2018, 2120, 3152, 3713, 4157, 4259
AcuI CTGAAG 5 cut(s) 1704, 1947, 2486, 3948, 4086
AdeI CACNNNGTG 2 cut(s) 327, 2559
AfaI GTAC 5 cut(s) 680, 2031, 2737, 2759, 2906
AflIII ACRYGT 2 cut(s) 995, 2496
AjiI CACGTC 6 cut(s) 133, 265, 2011, 2497, 2925, 4150
AjnI CCWGG 3 cut(s) 9, 161, 1971
AleI CACNNNNGTG 3 cut(s) 1782, 1887, 3250
Alw26I GTCTC 3 cut(s) 365, 480, 500
Alw44I GTGCAC 3 cut(s) 244, 319, 2551
AlwI GGATC 4 cut(s) 1222, 1963, 1976, 3466
AlwNI CAGNNNCTG 2 cut(s) 1059, 2985
Ama87I CYCGRG 2 cut(s) 36, 728
Aor13HI TCCGGA 2 cut(s) 1965, 4104
ApaLI GTGCAC 3 cut(s) 244, 319, 2551
ApoI RAATTY 8 cut(s) 99, 1469, 2018, 2120, 3152, 3713, 4157, 4259
Asp700I GAANNNNTTC 2 cut(s) 2283, 4422
Asp718I GGTACC 1 cut(s) 2735
AspA2I CCTAGG 1 cut(s) 4553
AspLEI GCGC 4 cut(s) 148, 472, 2312, 4565
AspS9I GGNCC 8 cut(s) 33, 1571, 1690, 1802, 1907, 3815, 3934, 4046
AsuC2I CCSGG 6 cut(s) 729, 730, 764, 1456, 2990, 3700
AvaI CYCGRG 2 cut(s) 36, 728
AvaII GGWCC 2 cut(s) 1571, 3815
AvrII CCTAGG 1 cut(s) 4553
BaeGI GKGCMC 3 cut(s) 248, 323, 2555
BaeI ACNNNNGTAYC 2 cut(s) 2719, 2752
BamHI GGATCC 1 cut(s) 1968
BanI GGYRCC 3 cut(s) 1946, 2735, 4085
BanII GRGCYC 6 cut(s) 256, 636, 799, 2464, 2488, 2865
BarI GAAGNNNNNNTAC 4 cut(s) 1620, 1652, 3864, 3896
BbsI GAAGAC 2 cut(s) 135, 1442
BbvCI CCTCAGC 4 cut(s) 411, 1806, 1911, 4050
BceAI ACGGC 6 cut(s) 33, 73, 1022, 2119, 3266, 4258
BciT130I CCWGG 3 cut(s) 11, 163, 1973
BciVI GTATCC 2 cut(s) 393, 2613
BclI TGATCA 2 cut(s) 2071, 4210
BcnI CCSGG 6 cut(s) 729, 730, 764, 1456, 2990, 3700
BcoDI GTCTC 3 cut(s) 365, 480, 500
BfaI CTAG 6 cut(s) 1083, 2714, 3228, 3327, 4466, 4554
BfmI CTRYAG 7 cut(s) 691, 1716, 1821, 2178, 2917, 3960, 4317
BfoI RGCGCY 2 cut(s) 149, 2313
BfuAI ACCTGC 6 cut(s) 568, 2171, 2797, 2997, 3250, 4310
BfuI GTATCC 2 cut(s) 393, 2613
BglI GCCNNNNNGGC 2 cut(s) 20, 2698
BlnI CCTAGG 1 cut(s) 4553
BmcAI AGTACT 1 cut(s) 2759
Bme1390I CCNGG 9 cut(s) 11, 163, 729, 730, 764, 1456, 1973, 2990, 3700
Bme18I GGWCC 2 cut(s) 1571, 3815
BmeT110I CYCGRG 2 cut(s) 36, 728
BmgBI CACGTC 6 cut(s) 133, 265, 2011, 2497, 2925, 4150
BmgT120I GGNCC 8 cut(s) 33, 1571, 1690, 1802, 1907, 3815, 3934, 4046
BmrFI CCNGG 9 cut(s) 11, 163, 729, 730, 764, 1456, 1973, 2990, 3700
BoxI GACNNNNGTC 4 cut(s) 1569, 2144, 3813, 4283
BpiI GAAGAC 2 cut(s) 135, 1442
BpmI CTGGAG 7 cut(s) 536, 2009, 2252, 2348, 2765, 4148, 4391
Bpu10I CCTNAGC 5 cut(s) 411, 1806, 1911, 2631, 4050
BpuMI CCSGG 6 cut(s) 729, 730, 764, 1456, 2990, 3700
Bsa29I ATCGAT 1 cut(s) 498
BsaAI YACGTR 2 cut(s) 620, 2849
BsaI GGTCTC 2 cut(s) 480, 500
BsaWI WCCGGW 2 cut(s) 1965, 4104
Bse1I ACTGG 5 cut(s) 2235, 2313, 3957, 4374, 4452
Bse3DI GCAATG 4 cut(s) 405, 1204, 2625, 3448
BseAI TCCGGA 2 cut(s) 1965, 4104
BseBI CCWGG 3 cut(s) 11, 163, 1973
BseCI ATCGAT 1 cut(s) 498
BseMI GCAATG 4 cut(s) 405, 1204, 2625, 3448
BseNI ACTGG 5 cut(s) 2235, 2313, 3957, 4374, 4452
BseRI GAGGAG 2 cut(s) 518, 3044
BseSI GKGCMC 3 cut(s) 248, 323, 2555
BseX3I CGGCCG 1 cut(s) 50
BseYI CCCAGC 4 cut(s) 1227, 1314, 3471, 3558
BsgI GTGCAG 3 cut(s) 430, 2650, 3228
Bsh1236I CGCG 3 cut(s) 432, 472, 2652
Bsh1285I CGRYCG 1 cut(s) 53
BshNI GGYRCC 3 cut(s) 1946, 2735, 4085
BshVI ATCGAT 1 cut(s) 498
BsiEI CGRYCG 1 cut(s) 53
BsiHKCI CYCGRG 2 cut(s) 36, 728
BsiSI CCGG 9 cut(s) 729, 763, 1456, 1562, 1966, 2989, 3700, 3806, 4105
BslFI GGGAC 4 cut(s) 315, 711, 1970, 4109
BsmAI GTCTC 3 cut(s) 365, 480, 500
BsmFI GGGAC 4 cut(s) 315, 711, 1970, 4109
BsmI GAATGC 2 cut(s) 846, 3096
Bso31I GGTCTC 2 cut(s) 480, 500
BsoBI CYCGRG 2 cut(s) 36, 728
Bsp13I TCCGGA 2 cut(s) 1965, 4104
BspDI ATCGAT 1 cut(s) 498
BspEI TCCGGA 2 cut(s) 1965, 4104
BspFNI CGCG 3 cut(s) 432, 472, 2652
BspMAI CTGCAG 4 cut(s) 695, 2182, 2921, 4321
BspMI ACCTGC 6 cut(s) 568, 2171, 2797, 2997, 3250, 4310
BspPI GGATC 4 cut(s) 1222, 1963, 1976, 3466
BspT107I GGYRCC 3 cut(s) 1946, 2735, 4085
BspTNI GGTCTC 2 cut(s) 480, 500
BsrDI GCAATG 4 cut(s) 405, 1204, 2625, 3448
BsrI ACTGG 5 cut(s) 2235, 2313, 3957, 4374, 4452
BssNAI GTATAC 2 cut(s) 1500, 3744
BssT1I CCWWGG 4 cut(s) 1118, 2490, 3362, 4553
Bst1107I GTATAC 2 cut(s) 1500, 3744
Bst2UI CCWGG 3 cut(s) 11, 163, 1973
Bst4CI ACNGT 9 cut(s) 1377, 2080, 2092, 2753, 3215, 3541, 3621, 4219, 4231
Bst6I CTCTTC 9 cut(s) 301, 554, 1166, 1829, 2274, 2533, 2564, 3011, 4413
BstAPI GCANNNNNTGC 5 cut(s) 453, 690, 852, 2673, 2916
BstBAI YACGTR 2 cut(s) 620, 2849
BstDSI CCRYGG 5 cut(s) 15, 430, 2135, 2650, 4274
BstENI CCTNNNNNAGG 2 cut(s) 166, 4557
BstFNI CGCG 3 cut(s) 432, 472, 2652
BstH2I RGCGCY 2 cut(s) 149, 2313
BstHHI GCGC 4 cut(s) 148, 472, 2312, 4565
BstMAI GTCTC 3 cut(s) 365, 480, 500
BstMCI CGRYCG 1 cut(s) 53
BstNI CCWGG 3 cut(s) 11, 163, 1973
BstNSI RCATGY 7 cut(s) 999, 2030, 2054, 2354, 3707, 4169, 4493
BstPAI GACNNNNGTC 4 cut(s) 1569, 2144, 3813, 4283
BstSCI CCNGG 9 cut(s) 9, 161, 727, 728, 762, 1454, 1971, 2988, 3698
BstSFI CTRYAG 7 cut(s) 691, 1716, 1821, 2178, 2917, 3960, 4317
BstSLI GKGCMC 3 cut(s) 248, 323, 2555
BstUI CGCG 3 cut(s) 432, 472, 2652
BstV2I GAAGAC 2 cut(s) 135, 1442
BstX2I RGATCY 1 cut(s) 1968
BstXI CCANNNNNNTGG 2 cut(s) 2156, 4295
BstYI RGATCY 1 cut(s) 1968
BstZ17I GTATAC 2 cut(s) 1500, 3744
BstZI CGGCCG 1 cut(s) 50
Bsu15I ATCGAT 1 cut(s) 498
BsuI GTATCC 2 cut(s) 393, 2613
BsuTUI ATCGAT 1 cut(s) 498
BtgI CCRYGG 5 cut(s) 15, 430, 2135, 2650, 4274
BtrI CACGTC 6 cut(s) 133, 265, 2011, 2497, 2925, 4150
BtsI GCAGTG 2 cut(s) 1639, 3883
BveI ACCTGC 6 cut(s) 568, 2171, 2797, 2997, 3250, 4310
CaiI CAGNNNCTG 2 cut(s) 1059, 2985
CfoI GCGC 4 cut(s) 148, 472, 2312, 4565
Cfr13I GGNCC 8 cut(s) 33, 1571, 1690, 1802, 1907, 3815, 3934, 4046
Cfr42I CCGCGG 2 cut(s) 433, 2653
Cfr9I CCCGGG 1 cut(s) 728
ClaI ATCGAT 1 cut(s) 498
CseI GACGC 1 cut(s) 792
Csp6I GTAC 5 cut(s) 679, 2030, 2736, 2758, 2905
CviQI GTAC 5 cut(s) 679, 2030, 2736, 2758, 2905
DraIII CACNNNGTG 2 cut(s) 327, 2559
EaeI YGGCCR 1 cut(s) 50
EagI CGGCCG 1 cut(s) 50
Eam1104I CTCTTC 9 cut(s) 301, 554, 1166, 1829, 2274, 2533, 2564, 3011, 4413
EarI CTCTTC 9 cut(s) 301, 554, 1166, 1829, 2274, 2533, 2564, 3011, 4413
EciI GGCGGA 1 cut(s) 193
Ecl136II GAGCTC 3 cut(s) 634, 797, 2863
EclXI CGGCCG 1 cut(s) 50
Eco130I CCWWGG 4 cut(s) 1118, 2490, 3362, 4553
Eco24I GRGCYC 6 cut(s) 256, 636, 799, 2464, 2488, 2865
Eco31I GGTCTC 2 cut(s) 480, 500
Eco47I GGWCC 2 cut(s) 1571, 3815
Eco52I CGGCCG 1 cut(s) 50
Eco53kI GAGCTC 3 cut(s) 634, 797, 2863
Eco57I CTGAAG 5 cut(s) 1704, 1947, 2486, 3948, 4086
Eco88I CYCGRG 2 cut(s) 36, 728
EcoICRI GAGCTC 3 cut(s) 634, 797, 2863
EcoNI CCTNNNNNAGG 2 cut(s) 166, 4557
EcoO109I RGGNCCY 1 cut(s) 33
EcoRI GAATTC 3 cut(s) 99, 2120, 4259
EcoRII CCWGG 3 cut(s) 9, 161, 1971
EcoT14I CCWWGG 4 cut(s) 1118, 2490, 3362, 4553
EcoT22I ATGCAT 3 cut(s) 908, 2028, 4167
EcoT38I GRGCYC 6 cut(s) 256, 636, 799, 2464, 2488, 2865
ErhI CCWWGG 4 cut(s) 1118, 2490, 3362, 4553
FalI AAGNNNNNCTT 4 cut(s) 1677, 1709, 3921, 3953
FaqI GGGAC 4 cut(s) 315, 711, 1970, 4109
FauI CCCGC 2 cut(s) 1242, 3486
FauNDI CATATG 1 cut(s) 3111
FbaI TGATCA 2 cut(s) 2071, 4210
FblI GTMKAC 3 cut(s) 1499, 2501, 3743
FriOI GRGCYC 6 cut(s) 256, 636, 799, 2464, 2488, 2865
FspBI CTAG 6 cut(s) 1083, 2714, 3228, 3327, 4466, 4554
GlaI GCGC 4 cut(s) 147, 471, 2311, 4564
GsaI CCCAGC 4 cut(s) 1231, 1318, 3475, 3562
GsuI CTGGAG 7 cut(s) 536, 2009, 2252, 2348, 2765, 4148, 4391
HaeII RGCGCY 2 cut(s) 149, 2313
HapII CCGG 9 cut(s) 729, 763, 1456, 1562, 1966, 2989, 3700, 3806, 4105
HgaI GACGC 1 cut(s) 792
HhaI GCGC 4 cut(s) 148, 472, 2312, 4565
Hin6I GCGC 4 cut(s) 146, 470, 2310, 4563
HinP1I GCGC 4 cut(s) 146, 470, 2310, 4563
HincII GTYRAC 1 cut(s) 2502
HindII GTYRAC 1 cut(s) 2502
HindIII AAGCTT 2 cut(s) 1634, 3878
HpaII CCGG 9 cut(s) 729, 763, 1456, 1562, 1966, 2989, 3700, 3806, 4105
Hpy99I CGWCG 3 cut(s) 113, 116, 2929
HpyAV CCTTC 9 cut(s) 212, 467, 2389, 2413, 2437, 2461, 2687, 2869, 4504
HpyCH4III ACNGT 9 cut(s) 1377, 2080, 2092, 2753, 3215, 3541, 3621, 4219, 4231
HpyCH4IV ACGT 9 cut(s) 132, 264, 619, 1447, 2010, 2496, 2848, 2924, 4149
HpySE526I ACGT 9 cut(s) 132, 264, 619, 1447, 2010, 2496, 2848, 2924, 4149
HspAI GCGC 4 cut(s) 146, 470, 2310, 4563
Kpn2I TCCGGA 2 cut(s) 1965, 4104
KpnI GGTACC 1 cut(s) 2739
Ksp22I TGATCA 2 cut(s) 2071, 4210
KspI CCGCGG 2 cut(s) 433, 2653
MaeI CTAG 6 cut(s) 1083, 2714, 3228, 3327, 4466, 4554
MaeII ACGT 9 cut(s) 132, 264, 619, 1447, 2010, 2496, 2848, 2924, 4149
MfeI CAATTG 3 cut(s) 120, 2681, 4026
MflI RGATCY 1 cut(s) 1968
MlyI GAGTC 2 cut(s) 1823, 1928
MmeI TCCRAC 5 cut(s) 1105, 1392, 2321, 3349, 4460
Mph1103I ATGCAT 3 cut(s) 908, 2028, 4167
MroI TCCGGA 2 cut(s) 1965, 4104
MroXI GAANNNNTTC 2 cut(s) 2283, 4422
MspI CCGG 9 cut(s) 729, 763, 1456, 1562, 1966, 2989, 3700, 3806, 4105
MspR9I CCNGG 9 cut(s) 11, 163, 729, 730, 764, 1456, 1973, 2990, 3700
MunI CAATTG 3 cut(s) 120, 2681, 4026
Mva1269I GAATGC 2 cut(s) 846, 3096
MvaI CCWGG 3 cut(s) 11, 163, 1973
MvnI CGCG 3 cut(s) 432, 472, 2652
NciI CCSGG 6 cut(s) 729, 730, 764, 1456, 2990, 3700
NdeI CATATG 1 cut(s) 3111
NmeAIII GCCGAG 2 cut(s) 109, 732
NmuCI GTSAC 6 cut(s) 620, 662, 1100, 1775, 1880, 4019
NsiI ATGCAT 3 cut(s) 908, 2028, 4167
NspI RCATGY 7 cut(s) 999, 2030, 2054, 2354, 3707, 4169, 4493
OliI CACNNNNGTG 3 cut(s) 1782, 1887, 3250
PaeI GCATGC 2 cut(s) 2354, 4493
PaqCI CACCTGC 4 cut(s) 568, 2797, 2997, 3250
PasI CCCWGGG 1 cut(s) 162
PciI ACATGT 1 cut(s) 995
PctI GAATGC 2 cut(s) 846, 3096
PdmI GAANNNNTTC 2 cut(s) 2283, 4422
PflMI CCANNNNNTGG 2 cut(s) 1124, 3368
PfoI TCCNGGA 1 cut(s) 1971
PleI GAGTC 2 cut(s) 1822, 1927
PpsI GAGTC 2 cut(s) 1822, 1927
Ppu21I YACGTR 2 cut(s) 620, 2849
PscI ACATGT 1 cut(s) 995
PshAI GACNNNNGTC 4 cut(s) 1569, 2144, 3813, 4283
PsiI TTATAA 2 cut(s) 1383, 3627
Psp124BI GAGCTC 3 cut(s) 636, 799, 2865
Psp6I CCWGG 3 cut(s) 9, 161, 1971
PspFI CCCAGC 4 cut(s) 1227, 1314, 3471, 3558
PspGI CCWGG 3 cut(s) 9, 161, 1971
PspPI GGNCC 8 cut(s) 33, 1571, 1690, 1802, 1907, 3815, 3934, 4046
PstI CTGCAG 4 cut(s) 695, 2182, 2921, 4321
PstNI CAGNNNCTG 2 cut(s) 1059, 2985
PsuI RGATCY 1 cut(s) 1968
RsaI GTAC 5 cut(s) 680, 2031, 2737, 2759, 2906
RsaNI GTAC 5 cut(s) 679, 2030, 2736, 2758, 2905
SacI GAGCTC 3 cut(s) 636, 799, 2865
SacII CCGCGG 2 cut(s) 433, 2653
SalI GTCGAC 1 cut(s) 2500
Sau96I GGNCC 8 cut(s) 33, 1571, 1690, 1802, 1907, 3815, 3934, 4046
SbfI CCTGCAGG 2 cut(s) 2182, 4321
ScaI AGTACT 1 cut(s) 2759
SchI GAGTC 2 cut(s) 1823, 1928
ScrFI CCNGG 9 cut(s) 11, 163, 729, 730, 764, 1456, 1973, 2990, 3700
SdaI CCTGCAGG 2 cut(s) 2182, 4321
SfcI CTRYAG 7 cut(s) 691, 1716, 1821, 2178, 2917, 3960, 4317
Sfr303I CCGCGG 2 cut(s) 433, 2653
SgrBI CCGCGG 2 cut(s) 433, 2653
SinI GGWCC 2 cut(s) 1571, 3815
SmaI CCCGGG 1 cut(s) 730
SphI GCATGC 2 cut(s) 2354, 4493
Sse8387I CCTGCAGG 2 cut(s) 2182, 4321
SspI AATATT 2 cut(s) 1666, 3910
SspMI CTAG 6 cut(s) 1083, 2714, 3228, 3327, 4466, 4554
SstI GAGCTC 3 cut(s) 636, 799, 2865
StyD4I CCNGG 9 cut(s) 9, 161, 727, 728, 762, 1454, 1971, 2988, 3698
StyI CCWWGG 4 cut(s) 1118, 2490, 3362, 4553
TaaI ACNGT 9 cut(s) 1377, 2080, 2092, 2753, 3215, 3541, 3621, 4219, 4231
TaiI ACGT 9 cut(s) 135, 267, 622, 1450, 2013, 2499, 2851, 2927, 4152
TatI WGTACW 4 cut(s) 678, 2029, 2757, 2904
TauI GCSGC 9 cut(s) 23, 52, 178, 236, 432, 435, 660, 2652, 2655
TseFI GTSAC 6 cut(s) 620, 662, 1100, 1775, 1880, 4019
Tsp45I GTSAC 6 cut(s) 620, 662, 1100, 1775, 1880, 4019
TspGWI ACGGA 8 cut(s) 129, 388, 681, 1386, 1615, 2291, 3630, 4430
TspMI CCCGGG 1 cut(s) 728
Van91I CCANNNNNTGG 2 cut(s) 1124, 3368
VneI GTGCAC 3 cut(s) 244, 319, 2551
VpaK11BI GGWCC 2 cut(s) 1571, 3815
XagI CCTNNNNNAGG 2 cut(s) 166, 4557
XapI RAATTY 8 cut(s) 99, 1469, 2018, 2120, 3152, 3713, 4157, 4259
XbaI TCTAGA 3 cut(s) 1082, 2713, 3326
XceI RCATGY 7 cut(s) 999, 2030, 2054, 2354, 3707, 4169, 4493
XcmI CCANNNNNNNNNTGG 4 cut(s) 1242, 1355, 3486, 3599
XmaI CCCGGG 1 cut(s) 728
XmaJI CCTAGG 1 cut(s) 4553
XmiI GTMKAC 3 cut(s) 1499, 2501, 3743
XmnI GAANNNNTTC 2 cut(s) 2283, 4422
XspI CTAG 6 cut(s) 1083, 2714, 3228, 3327, 4466, 4554
ZrmI AGTACT 1 cut(s) 2759
Zsp2I ATGCAT 3 cut(s) 908, 2028, 4167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.