MD03G1019500.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000130
Physical Location & Seq
Forward (+)
1496758 .. 1499090
2333 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1019500.v1.1.491

Sequence Viewer

Length: 1671 bp
ATGGCAATGGCTTTTTCTGTGCGGATGCTGTTGTTCGCCTCATTGTTTTTGCTACCAATTTCTGTGTTTTCCCAAAATAATGGAAGAGTAGCAGTTGGAAGTTCTCTAACTGCAACTACAAGTAACTCCACATCATGGCTTTCTCCATCCGGTGATTTCGCGTTTGGATTTTTGCCCCTTGGAGGCAATGATCTTTTCTTGCTTTCAATATGGTACGCCAAAATCCCAGACAAAACCGTTGTTTGGCATGCATATGAGGATAACAACCCCATAGTTGCACCTGAGGGATCAACTTTGAACTTGGATGCCAGCAGTGGGCTAGTTCTTAACAATCCTCAAGGTGGGGAGATATGGAAATCCGCAACTTTCGGGGTTGTTGCAAACGGGGTCATGAATGACACCGGAAACTTTGTTCTTCAAGACAAAAACTCGGGGAGCTTATGGGAGACGTTCAACAATCCTACAGACACCATTTTGCCGGGGCAGACAATTGAGAGAAATGGGAAGCTTTCGTCTAAACAATCGGAGAGTAACTACGCGAAGGGGCGGTTCCAGCTGCGCTTGCAAGATGATGGAAACCTCGTGCTCAACACCATAAACTTGCCAACAGATTTTGCCAACGAGCCTTACTTTGCCACGGACACAACAGCGGGGACTGTGGAAGGTAGTGAAGGTAAACAATTGGTGTTCAACAACTCAGGGGACTTGTTTGTTCTGAGAGAAAATGGTGGAAGATTCATTCTCACAACGGCAAAGGGAGTCTCAGCGAGGGAGAACTACCTAAGGGCAACTCTTGATTTTGATGGGATTTTCGCGCTATATGCTCACCCAAAAACTTTCACCGGAAATGCAAGCTGGAGTAGTCCTCTGTGGTATCAGCCGGTTGATATTTGCCAAACACTTTTGGAAGGCGCGGGCGTTGGTGTTTGCGGATACAACAGTATCTGCAAGCTCAAACCAGATAATAGGCCGACCTGCGAATGCCCGAAAGGGTTTACTTTTCTTGATCCCAAGGACTTATACCGAGGCTGCAAACCCGATTTTATACAAGGCTGTAAAGAAGATGAGCTAGCAAGTCCCAGGCAAGATTTGTATGATGTCGAGGTGCTGATAAATACTGATTGGCCAACCTCAGATTATGTGCAGCTAAAGCCTTTTACCGCGGACAAGTGCAACGAGTCTTGCTTTCATGATTGTTTGTGCGCTGTTGCTGTTTTCCGGTCTGAAACCTGCTGGAAAAAGAAGTTACCTCTTTCGAACGGGAGAGTGGATGTCAGTCTTAATTCGCAGACCTTCATCAAAGAAAGGGTGTATTTGGAGTGGTTTCAAAGGGGGATGCAAATTGGTTCTGGTGTCGAAGTGGCGGTGAAGAGGCTAAACTATGTTATGCAAGATGTCGAGGAGTTCAAAACAGAACTGAATGTAATTAGTCAGACGCATCACAAGAATCTGGTTCGTCTTTTCGGATATTGTGACAAGGGGCAACAAAGATCACTAGTTTTTGAGTTCATGAGCAATGGAACATTGGCAAGCTTTATTTTTGCTGATATCAAACCAAGTTGGAGACAGCGAATTGAAATCACTTATGGCGTTGCTAAGGGGCTTTTGTACTTGTCAATAGAACACGAGGGAGTAGAGAGGGCAGCAATAATTCCAATTCATACATGGTAG

Protein Analysis

557

Amino Acids

61.82

Weight (kDa)

5.38

Isoelectric Point (pI)

31.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 79 - 173 1.4e-13 D-mannose binding lectin
Pkinase PF00069 445 - 538 3.7e-09 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 449 - 539 1.2e-14 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000130)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g43780
malus_domestica MD03G1019500.v1.1 MD03G1019800.v1.1 MD03G1019900.v1.1 MD03G1020000.v1.1 MD03G1020200.v1.1 MD03G1020300.v1.1 MD05G1226500.v1.1 MD05G1226600.v1.1 MD05G1226700.v1.1 MD05G1226800.v1.1 MD09G1060200.v1.1 MD11G1021100.v1.1 MD11G1021300.v1.1 MD11G1021400.v1.1 MD11G1021500.v1.1 MD11G1024600.v1.1 MD11G1026700.v1.1
prunus_persica Prupe.6G016300_v2.0.a1 Prupe.6G016600_v2.0.a1 Prupe.6G016700_v2.0.a1 Prupe.6G017000_v2.0.a1 Prupe.6G017100_v2.0.a1 Prupe.6G017200_v2.0.a1 Prupe.6G017300_v2.0.a1 Prupe.6G017400_v2.0.a1 Prupe.6G017500_v2.0.a1
pyrus_communis pycom02g26630 pycom03g01670 pycom03g01710 pycom03g01720 pycom05g20850 pycom11g01710 pycom11g01730 pycom11g01740 pycom11g01960
rosa_chinensis RchiOBHm_Chr1g0313471 RchiOBHm_Chr1g0313491 RchiOBHm_Chr1g0313521 RchiOBHm_Chr1g0329041 RchiOBHm_Chr5g0077581 RchiOBHm_Chr5g0077681 RchiOBHm_Chr5g0077751 RchiOBHm_Chr5g0077771 RchiOBHm_Chr5g0078381 RchiOBHm_Chr5g0078421 RchiOBHm_Chr5g0078481 RchiOBHm_Chr5g0078491 RchiOBHm_Chr5g0078511 RchiOBHm_Chr5g0078561 RchiOBHm_Chr5g0078581 RchiOBHm_Chr5g0078621 RchiOBHm_Chr5g0078631 RchiOBHm_Chr5g0078641 RchiOBHm_Chr5g0078681 RchiOBHm_Chr5g0078741 RchiOBHm_Chr5g0078781 RchiOBHm_Chr5g0078791 RchiOBHm_Chr5g0078821 RchiOBHm_Chr5g0078851 RchiOBHm_Chr5g0078861 RchiOBHm_Chr5g0078881 RchiOBHm_Chr5g0078911 RchiOBHm_Chr5g0078921 RchiOBHm_Chr5g0078931 RchiOBHm_Chr5g0078951 RchiOBHm_Chr5g0079061 RchiOBHm_Chr5g0079071 RchiOBHm_Chr5g0079101 RchiOBHm_Chr5g0079111 RchiOBHm_Chr5g0079131 RchiOBHm_Chr5g0079151 RchiOBHm_Chr5g0079161 RchiOBHm_Chr5g0079211 RchiOBHm_Chr5g0079241 RchiOBHm_Chr7g0223671
rosa_laevigata RLG00000001266 RLG00000036708 RLG00000036719 RLG00000036772 RLG00000036773 RLG00000036776 RLG00000036780 RLG00000036781 RLG00000036783 RLG00000036788 RLG00000036790 RLG00000036792 RLG00000036794 RLG00000036797 RLG00000036798 RLG00000036800 RLG00000036801 RLG00000036802 RLG00000036803 RLG00000036805 RLG00000036807 RLG00000036808
rosa_multiflora Rmu_co7998958.1_g000001 Rmu_co8175864.1_g000001 Rmu_co8214884.1_g000001 Rmu_co8225446.1_g000001 Rmu_sc0000607.1_g000011 Rmu_sc0000607.1_g000012 Rmu_sc0000631.1_g000001 Rmu_sc0000631.1_g000003 Rmu_sc0000631.1_g000010 Rmu_sc0000631.1_g000012 Rmu_sc0000631.1_g000020 Rmu_sc0001347.1_g000014 Rmu_sc0002187.1_g000011 Rmu_sc0002187.1_g000014 Rmu_sc0002187.1_g000024 Rmu_sc0002187.1_g000025 Rmu_sc0003413.1_g000051 Rmu_sc0004140.1_g000002 Rmu_sc0004140.1_g000003 Rmu_sc0004140.1_g000015 Rmu_sc0004140.1_g000026 Rmu_sc0004140.1_g000036 Rmu_sc0004140.1_g000042 Rmu_sc0004140.1_g000046 Rmu_sc0004140.1_g000048 Rmu_sc0004277.1_g000002 Rmu_sc0008700.1_g000002 Rmu_sc0011280.1_g000001 Rmu_sc0011280.1_g000002 Rmu_sc0011280.1_g000009 Rmu_sc0012487.1_g000003 Rmu_sc0012487.1_g000007 Rmu_sc0012487.1_g000009 Rmu_sc0012931.1_g000001 Rmu_sc0012931.1_g000002 Rmu_sc0012931.1_g000009 Rmu_sc0015122.1_g000002 Rmu_sc0022488.1_g000001 Rmu_ssc0000425.1_g000018
rosa_roxburghii Rroxscaffold_164G00436380 Rroxscaffold_1G00003360 Rroxscaffold_1G00003370 Rroxscaffold_1G00003660 Rroxscaffold_1G00003790 Rroxscaffold_1G00003840 Rroxscaffold_1G00003950 Rroxscaffold_1G00004380 Rroxscaffold_4G00332170
rosa_rugosa Rorug05G0455800 Rorug05G0456500 Rorug05G0460800 Rorug05G0461000 Rorug05G0461200 Rorug05G0461200 Rorug05G0461300 Rorug05G0461600 Rorug05G0461700 Rorug05G0461900 Rorug05G0462000 Rorug05G0462400 Rorug05G0462500 Rorug05G0462800 Rorug05G0463200.1 Rorug05G0463300
rosa_samantha Rh1CG000600 Rh1CG008300 Rh1CG360600 Rh5AG509700 Rh5AG509800 Rh5AG510700 Rh5AG515000 Rh5AG515600 Rh5BG531300 Rh5BG531400 Rh5BG533600 Rh5BG538200 Rh5BG538300 Rh5BG538400 Rh5BG538500 Rh5BG539100 Rh5BG539200 Rh5BG539400 Rh5DG545100 Rh5DG549000 Rh5DG549200 Rh5DG549500 Rh5DG549600 Rh5DG549800 Rh5DG549900 Rh5DG550000 Rh5DG550200 Rh5DG550300 Rh7AG436900 Rh7CG456800
rosa_wichuraiana Rw0G003110 Rw0G003120 Rw0G005240 Rw0G023800 Rw0G023830 Rw1G007840 Rw5G000030 Rw5G047760 Rw5G047780 Rw5G047810 Rw5G047900 Rw5G047920 Rw5G047970 Rw5G047980 Rw5G048000 Rw5G048010 Rw5G048030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 983, 1238
AccB7I CCANNNNNTGG 1 cut(s) 135
AccII CGCG 5 cut(s) 161, 539, 815, 914, 1163
AciI CCGC 9 cut(s) 22, 360, 547, 650, 914, 930, 1161, 1163, 1364
AclWI GGATC 2 cut(s) 295, 1001
AcoI YGGCCR 1 cut(s) 1124
AfaI GTAC 2 cut(s) 215, 1610
AfiI CCNNNNNNNGG 5 cut(s) 135, 182, 243, 315, 341
AgsI TTSAA 8 cut(s) 207, 298, 419, 454, 691, 1328, 1408, 1577
AhlI ACTAGT 1 cut(s) 1495
AjnI CCWGG 1 cut(s) 1079
AloI GAACNNNNNNTCC 2 cut(s) 396, 428
AluBI AGCT 8 cut(s) 438, 508, 556, 855, 952, 1069, 1147, 1533
AluI AGCT 8 cut(s) 438, 508, 556, 855, 952, 1069, 1147, 1533
Alw21I GWGCWC 1 cut(s) 588
Alw26I GTCTC 3 cut(s) 440, 766, 1558
AlwI GGATC 2 cut(s) 295, 1001
AlwNI CAGNNNCTG 1 cut(s) 945
Ama87I CYCGRG 1 cut(s) 430
AoxI GGCC 2 cut(s) 968, 1124
ApeKI GCWGC 4 cut(s) 556, 1029, 1144, 1643
AspLEI GCGC 4 cut(s) 561, 817, 914, 1205
AsuC2I CCSGG 1 cut(s) 480
AsuHPI GGTGA 4 cut(s) 164, 818, 832, 1378
AsuII TTCGAA 1 cut(s) 1256
AsuNHI GCTAGC 1 cut(s) 1069
AvaI CYCGRG 1 cut(s) 430
AxyI CCTNAGG 2 cut(s) 282, 782
BaeI ACNNNNGTAYC 4 cut(s) 925, 925, 958, 958
BalI TGGCCA 1 cut(s) 1126
BauI CACGAG 2 cut(s) 581, 1625
Bbv12I GWGCWC 1 cut(s) 588
BbvI GCAGC 4 cut(s) 543, 1016, 1156, 1655
BccI CCATC 3 cut(s) 154, 566, 797
BceAI ACGGC 1 cut(s) 765
BciT130I CCWGG 1 cut(s) 1081
BciVI GTATCC 1 cut(s) 926
BcnI CCSGG 1 cut(s) 480
BcoDI GTCTC 3 cut(s) 440, 766, 1558
BcuI ACTAGT 1 cut(s) 1495
BfaI CTAG 3 cut(s) 320, 1070, 1496
BfmI CTRYAG 1 cut(s) 462
BfuAI ACCTGC 2 cut(s) 983, 1238
BfuI GTATCC 1 cut(s) 926
BisI GCNGC 4 cut(s) 557, 1030, 1145, 1644
BlsI GCNGC 4 cut(s) 558, 1031, 1146, 1645
Bme1390I CCNGG 2 cut(s) 480, 1081
BmeT110I CYCGRG 1 cut(s) 430
BmiI GGNNCC 1 cut(s) 551
BmrFI CCNGG 2 cut(s) 480, 1081
BmsI GCATC 4 cut(s) 15, 295, 1326, 1447
BmtI GCTAGC 1 cut(s) 1073
BplI GAGNNNNNCTC 2 cut(s) 850, 882
BpmI CTGGAG 1 cut(s) 877
Bpu10I CCTNAGC 1 cut(s) 1596
Bpu14I TTCGAA 1 cut(s) 1256
BpuEI CTTGAG 1 cut(s) 321
BpuMI CCSGG 1 cut(s) 480
BsaJI CCNNGG 7 cut(s) 178, 479, 636, 1011, 1024, 1079, 1161
BsaWI WCCGGW 4 cut(s) 149, 401, 842, 1218
Bsc4I CCNNNNNNNGG 5 cut(s) 135, 182, 243, 315, 341
Bse118I RCCGGY 1 cut(s) 880
Bse21I CCTNAGG 2 cut(s) 282, 782
Bse3DI GCAATG 3 cut(s) 12, 193, 1522
BseBI CCWGG 1 cut(s) 1081
BseDI CCNNGG 7 cut(s) 178, 479, 636, 1011, 1024, 1079, 1161
BseGI GGATG 5 cut(s) 30, 146, 310, 1276, 1341
BseLI CCNNNNNNNGG 5 cut(s) 135, 182, 243, 315, 341
BseMI GCAATG 3 cut(s) 12, 193, 1522
BseMII CTCAG 5 cut(s) 273, 707, 711, 777, 1146
BseRI GAGGAG 1 cut(s) 1415
BseXI GCAGC 4 cut(s) 543, 1016, 1156, 1655
BsgI GTGCAG 1 cut(s) 1163
Bsh1236I CGCG 5 cut(s) 161, 539, 815, 914, 1163
BshFI GGCC 2 cut(s) 970, 1126
BsiHKAI GWGCWC 1 cut(s) 588
BsiHKCI CYCGRG 1 cut(s) 430
BsiSI CCGG 6 cut(s) 150, 402, 479, 843, 881, 1219
BslFI GGGAC 3 cut(s) 667, 716, 1062
BslI CCNNNNNNNGG 5 cut(s) 135, 182, 243, 315, 341
BsmAI GTCTC 3 cut(s) 440, 766, 1558
BsmBI CGTCTC 1 cut(s) 440
BsmFI GGGAC 3 cut(s) 667, 716, 1062
BsmI GAATGC 1 cut(s) 986
BsnI GGCC 2 cut(s) 970, 1126
BsoBI CYCGRG 1 cut(s) 430
Bsp119I TTCGAA 1 cut(s) 1256
Bsp1286I GDGCHC 1 cut(s) 588
Bsp143I GATC 4 cut(s) 190, 287, 1006, 1490
BspACI CCGC 9 cut(s) 22, 360, 547, 650, 914, 930, 1161, 1163, 1364
BspANI GGCC 2 cut(s) 970, 1126
BspCNI CTCAG 5 cut(s) 274, 708, 710, 776, 1145
BspFNI CGCG 5 cut(s) 161, 539, 815, 914, 1163
BspHI TCATGA 3 cut(s) 390, 1189, 1509
BspLI GGNNCC 1 cut(s) 551
BspMI ACCTGC 2 cut(s) 983, 1238
BspOI GCTAGC 1 cut(s) 1073
BspPI GGATC 2 cut(s) 295, 1001
BspT104I TTCGAA 1 cut(s) 1256
BsrDI GCAATG 3 cut(s) 12, 193, 1522
BsrFI RCCGGY 1 cut(s) 880
BssAI RCCGGY 1 cut(s) 880
BssECI CCNNGG 7 cut(s) 178, 479, 636, 1011, 1024, 1079, 1161
BssMI GATC 4 cut(s) 190, 287, 1006, 1490
BssSI CACGAG 2 cut(s) 581, 1625
BssT1I CCWWGG 2 cut(s) 178, 1011
Bst2BI CACGAG 2 cut(s) 581, 1625
Bst2UI CCWGG 1 cut(s) 1081
Bst4CI ACNGT 3 cut(s) 238, 658, 941
Bst6I CTCTTC 2 cut(s) 79, 1364
BstBI TTCGAA 1 cut(s) 1256
BstC8I GCNNGC 8 cut(s) 249, 310, 563, 853, 916, 950, 1071, 1531
BstDEI CTNAG 7 cut(s) 282, 697, 716, 763, 782, 1132, 1596
BstDSI CCRYGG 2 cut(s) 636, 1161
BstF5I GGATG 5 cut(s) 30, 146, 310, 1276, 1341
BstFNI CGCG 5 cut(s) 161, 539, 815, 914, 1163
BstHHI GCGC 4 cut(s) 561, 817, 914, 1205
BstKTI GATC 4 cut(s) 193, 290, 1009, 1493
BstMAI GTCTC 3 cut(s) 440, 766, 1558
BstMBI GATC 4 cut(s) 190, 287, 1006, 1490
BstMWI GCNNNNNNNGC 4 cut(s) 553, 562, 821, 1150
BstNI CCWGG 1 cut(s) 1081
BstNSI RCATGY 1 cut(s) 251
BstSCI CCNGG 2 cut(s) 478, 1079
BstSFI CTRYAG 1 cut(s) 462
BstUI CGCG 5 cut(s) 161, 539, 815, 914, 1163
BstV1I GCAGC 4 cut(s) 543, 1016, 1156, 1655
BstXI CCANNNNNNTGG 1 cut(s) 80
Bsu36I CCTNAGG 2 cut(s) 282, 782
BsuI GTATCC 1 cut(s) 926
BsuRI GGCC 2 cut(s) 970, 1126
BtgI CCRYGG 2 cut(s) 636, 1161
BtsCI GGATG 5 cut(s) 30, 146, 310, 1276, 1341
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 1 cut(s) 319
BveI ACCTGC 2 cut(s) 983, 1238
Cac8I GCNNGC 8 cut(s) 249, 310, 563, 853, 916, 950, 1071, 1531
CaiI CAGNNNCTG 1 cut(s) 945
CciI TCATGA 3 cut(s) 390, 1189, 1509
CfoI GCGC 4 cut(s) 561, 817, 914, 1205
Cfr10I RCCGGY 1 cut(s) 880
Cfr42I CCGCGG 1 cut(s) 1164
CseI GACGC 1 cut(s) 1444
Csp6I GTAC 2 cut(s) 214, 1609
CviAII CATG 6 cut(s) 135, 248, 391, 1190, 1510, 1665
CviQI GTAC 2 cut(s) 214, 1609
DdeI CTNAG 7 cut(s) 282, 697, 716, 763, 782, 1132, 1596
DpnI GATC 4 cut(s) 192, 289, 1008, 1492
DpnII GATC 4 cut(s) 190, 287, 1006, 1490
EaeI YGGCCR 1 cut(s) 1124
Eam1104I CTCTTC 2 cut(s) 79, 1364
EarI CTCTTC 2 cut(s) 79, 1364
Eco130I CCWWGG 2 cut(s) 178, 1011
Eco32I GATATC 1 cut(s) 1549
Eco81I CCTNAGG 2 cut(s) 282, 782
Eco88I CYCGRG 1 cut(s) 430
EcoRII CCWGG 1 cut(s) 1079
EcoRV GATATC 1 cut(s) 1549
EcoT14I CCWWGG 2 cut(s) 178, 1011
EcoT22I ATGCAT 1 cut(s) 253
ErhI CCWWGG 2 cut(s) 178, 1011
Esp3I CGTCTC 1 cut(s) 440
FaeI CATG 6 cut(s) 138, 251, 394, 1193, 1513, 1668
FaqI GGGAC 3 cut(s) 667, 716, 1062
FatI CATG 6 cut(s) 134, 247, 390, 1189, 1509, 1664
FauI CCCGC 2 cut(s) 643, 907
FauNDI CATATG 1 cut(s) 253
Fnu4HI GCNGC 4 cut(s) 557, 1030, 1145, 1644
FokI GGATG 5 cut(s) 37, 133, 317, 1283, 1348
Fsp4HI GCNGC 4 cut(s) 557, 1030, 1145, 1644
FspBI CTAG 3 cut(s) 320, 1070, 1496
GlaI GCGC 4 cut(s) 560, 816, 913, 1204
GluI GCNGC 4 cut(s) 557, 1030, 1145, 1644
GsuI CTGGAG 1 cut(s) 877
HaeIII GGCC 2 cut(s) 970, 1126
HapII CCGG 6 cut(s) 150, 402, 479, 843, 881, 1219
HgaI GACGC 1 cut(s) 1444
HhaI GCGC 4 cut(s) 561, 817, 914, 1205
Hin1II CATG 6 cut(s) 138, 251, 394, 1193, 1513, 1668
Hin6I GCGC 4 cut(s) 559, 815, 912, 1203
HinP1I GCGC 4 cut(s) 559, 815, 912, 1203
HindIII AAGCTT 2 cut(s) 506, 1531
HinfI GANTC 4 cut(s) 735, 759, 1178, 1447
HpaII CCGG 6 cut(s) 150, 402, 479, 843, 881, 1219
HphI GGTGA 4 cut(s) 164, 818, 832, 1378
Hpy166II GTNNAC 2 cut(s) 677, 996
Hpy188I TCNGA 6 cut(s) 526, 717, 1135, 1225, 1434, 1466
Hpy188III TCNNGA 6 cut(s) 391, 419, 794, 1004, 1190, 1510
Hpy8I GTNNAC 2 cut(s) 677, 996
HpyAV CCTTC 5 cut(s) 535, 656, 665, 902, 1303
HpyCH4III ACNGT 3 cut(s) 238, 658, 941
HpyCH4IV ACGT 1 cut(s) 449
HpyF10VI GCNNNNNNNGC 4 cut(s) 553, 562, 821, 1150
HpyF3I CTNAG 7 cut(s) 282, 697, 716, 763, 782, 1132, 1596
HpySE526I ACGT 1 cut(s) 449
Hsp92II CATG 6 cut(s) 138, 251, 394, 1193, 1513, 1668
HspAI GCGC 4 cut(s) 559, 815, 912, 1203
KspI CCGCGG 1 cut(s) 1164
Kzo9I GATC 4 cut(s) 190, 287, 1006, 1490
LmnI GCTCC 1 cut(s) 435
Lsp1109I GCAGC 4 cut(s) 543, 1016, 1156, 1655
LweI GCATC 4 cut(s) 15, 295, 1326, 1447
MaeI CTAG 3 cut(s) 320, 1070, 1496
MaeII ACGT 1 cut(s) 449
MaeIII GTNAC 4 cut(s) 122, 530, 1245, 1472
MalI GATC 4 cut(s) 192, 289, 1008, 1492
MboI GATC 4 cut(s) 190, 287, 1006, 1490
MboII GAAGA 5 cut(s) 96, 407, 744, 1073, 1381
MfeI CAATTG 2 cut(s) 489, 680
MhlI GDGCHC 1 cut(s) 588
MlsI TGGCCA 1 cut(s) 1126
MluCI AATT 9 cut(s) 57, 489, 680, 1282, 1341, 1425, 1572, 1650, 1656
MluNI TGGCCA 1 cut(s) 1126
MlyI GAGTC 2 cut(s) 768, 1187
MmeI TCCRAC 2 cut(s) 76, 1541
Mox20I TGGCCA 1 cut(s) 1126
Mph1103I ATGCAT 1 cut(s) 253
MscI TGGCCA 1 cut(s) 1126
MseI TTAA 2 cut(s) 327, 1281
MslI CAYNNNNRTG 1 cut(s) 252
Msp20I TGGCCA 1 cut(s) 1126
MspA1I CMGCKG 3 cut(s) 556, 650, 1163
MspI CCGG 6 cut(s) 150, 402, 479, 843, 881, 1219
MspR9I CCNGG 2 cut(s) 480, 1081
MunI CAATTG 2 cut(s) 489, 680
Mva1269I GAATGC 1 cut(s) 986
MvaI CCWGG 1 cut(s) 1081
MvnI CGCG 5 cut(s) 161, 539, 815, 914, 1163
MwoI GCNNNNNNNGC 4 cut(s) 553, 562, 821, 1150
NciI CCSGG 1 cut(s) 480
NdeI CATATG 1 cut(s) 253
NdeII GATC 4 cut(s) 190, 287, 1006, 1490
NheI GCTAGC 1 cut(s) 1069
NlaIII CATG 6 cut(s) 138, 251, 394, 1193, 1513, 1668
NlaIV GGNNCC 1 cut(s) 551
NmuCI GTSAC 1 cut(s) 1472
NsiI ATGCAT 1 cut(s) 253
NspI RCATGY 1 cut(s) 251
NspV TTCGAA 1 cut(s) 1256
PaeI GCATGC 1 cut(s) 251
PagI TCATGA 3 cut(s) 390, 1189, 1509
PctI GAATGC 1 cut(s) 986
PfeI GAWTC 2 cut(s) 735, 1447
PflMI CCANNNNNTGG 1 cut(s) 135
PkrI GCNGC 4 cut(s) 558, 1031, 1146, 1645
PleI GAGTC 2 cut(s) 767, 1186
PpsI GAGTC 2 cut(s) 767, 1186
Psp6I CCWGG 1 cut(s) 1079
PspGI CCWGG 1 cut(s) 1079
PspN4I GGNNCC 1 cut(s) 551
PstNI CAGNNNCTG 1 cut(s) 945
PvuII CAGCTG 1 cut(s) 556
RsaI GTAC 2 cut(s) 215, 1610
RsaNI GTAC 2 cut(s) 214, 1609
RseI CAYNNNNRTG 1 cut(s) 252
SacII CCGCGG 1 cut(s) 1164
SaqAI TTAA 2 cut(s) 327, 1281
SatI GCNGC 4 cut(s) 557, 1030, 1145, 1644
Sau3AI GATC 4 cut(s) 190, 287, 1006, 1490
SchI GAGTC 2 cut(s) 768, 1187
ScrFI CCNGG 2 cut(s) 480, 1081
SduI GDGCHC 1 cut(s) 588
SfaNI GCATC 4 cut(s) 15, 295, 1326, 1447
SfcI CTRYAG 1 cut(s) 462
Sfr303I CCGCGG 1 cut(s) 1164
SfuI TTCGAA 1 cut(s) 1256
SgrBI CCGCGG 1 cut(s) 1164
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 1 cut(s) 336
SmoI CTYRAG 1 cut(s) 336
SpeI ACTAGT 1 cut(s) 1495
SphI GCATGC 1 cut(s) 251
Sse9I AATT 9 cut(s) 57, 489, 680, 1282, 1341, 1425, 1572, 1650, 1656
SsiI CCGC 9 cut(s) 22, 360, 547, 650, 914, 930, 1161, 1163, 1364
SspMI CTAG 3 cut(s) 320, 1070, 1496
StyD4I CCNGG 2 cut(s) 478, 1079
StyI CCWWGG 2 cut(s) 178, 1011
TaaI ACNGT 3 cut(s) 238, 658, 941
TaiI ACGT 1 cut(s) 452
TaqI TCGA 4 cut(s) 1101, 1256, 1356, 1398
TasI AATT 9 cut(s) 57, 489, 680, 1282, 1341, 1425, 1572, 1650, 1656
TatI WGTACW 1 cut(s) 1608
TfiI GAWTC 2 cut(s) 735, 1447
Tru1I TTAA 2 cut(s) 327, 1281
Tru9I TTAA 2 cut(s) 327, 1281
TscAI CASTG 1 cut(s) 319
TseFI GTSAC 1 cut(s) 1472
TseI GCWGC 4 cut(s) 556, 1029, 1144, 1643
Tsp45I GTSAC 1 cut(s) 1472
TspDTI ATGAA 6 cut(s) 407, 727, 1178, 1285, 1498, 1649
TspGWI ACGGA 1 cut(s) 653
TspRI CASTG 1 cut(s) 319
Van91I CCANNNNNTGG 1 cut(s) 135
XceI RCATGY 1 cut(s) 251
XcmI CCANNNNNNNNNTGG 1 cut(s) 1662
XspI CTAG 3 cut(s) 320, 1070, 1496
Zsp2I ATGCAT 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.