RchiOBHm_Chr5g0078791

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000130
Physical Location & Seq
Reverse (-)
84589054 .. 84592066
3013 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35328

Sequence Viewer

Length: 2415 bp
ATGGCAATGCTTTTTACTCTATGGCTGCTTATGCTTCTGCTACCAATTTATGTGCTTGCACAAACTAATGGTAGCGTAGCAGTCGGTGCTTCTCTCTCTGCAGCAGGTAACTCCTCATGGATTTCTCCATCTGGTGACTTTGCTTTTGGATTTCGGCAACTTGAAAACAATGGACTTTTCTTGCTTTCCATATGGTTTGCCAAGATACCAGACAAAACTATAGTTTGGTACGCAAATGGGGATACGCCTGCACCTAAGGGATCAACAGTGAACTTGACTGCCAACAGTGGATTAGTCCTTACAAGTCCTCAAGGTGAGAAGCTATGGACATCCAATGACACTAACATTATTGCTGGTGTCGTTGCTCATGGGGTCATGAATGATGCCGGTAACTTTGTTCTGGAAAATGCGAAGTCAGCAAAGTTATGGGAGACGTTCAAGAATCCTAGTGATACAATGTTGCCCGGGCAAATAATGGAAAGAGGAGGGAAACTGTCTTCTCGAAATTCAGAGACTGACTATTCCGGAGGGCGCTTCCAGCTAGATTTTCAACCAGATGGGAATCTTGTGTTCACTGCTAAGAAACCTTTTCATTCAACTGCAACTACCACTGGGACTGTTCCAGGTAGTGAAGGTCTACAACTGGTTTTCAATGAGTCGGGCAAGTTCTATATTCTGCGAGTAAATCATGGACTTTTCACTTTCTCAGGGGAGGAACGTTCAGCAAGGGACTACTATCTCCGTACAACTCTCAGCTTTGATGGGGTTTTGGCTCAATATTTCCTCCCAAAGACTTCCATTGGCAATGAAAGCTGGGCTGCTCTTTGGTCAGAGCCGCCTAATATTTGCCAAAAAATTAAGATGAATTCGGGTCCTGGTATTTGTGGTTACAACAGTATCTGCACTCTCAAAGATAAGAGGCCATCCTGTGAATGCCTAACAGGATACTCTTTACTTGATCCAGCAGATGCATACGGGAACTGTTATCCAGATTCCAAACAAGGCTGTGAAGATGAGCTTAGTTATACAAAAGATCTATATGATGTCAAGAAAGCTATGTTATGGGGGCATCTTGGCAAGGGCGGAAAGGTGGCTTCCCGGTGGCGGATTTGCGACTTCCGGATACCGCCGCGTAGCCTGAACATAGGGCTACACGATGCATGTCTCGGTTGGGCCTTGCCATGTCTTGTGGATGTCCCAAATCAATCTTGCTTGGAAGATTGTTTGTGTGCTGTTGCTGTTTACGGCAATGAAACCTGTTGGAAAAAGAAGTTTCCACTCTCATATGGAAGAGAAGACACCACCCTTAATGCAAAGACCTTCATCAAATTCAGGAATGATACTTCAGCTCTACAAGTTCCGGATGATAAGAAGAAGAGCCAGACCACTAGGGTACGTTTGTTGTCAGTTCTTCTGAGCACCTCTGTCTTTGGTAATTTTATATTAGCTGCTGCAGTTATTTTTCTGGGAATTTTTTTCACTTTCCGGAAGAAACATGTAAGGTCGTTTGCGCAAAATGATTTGGACACAAAATTGCGTTTCTTTAGTCAGAAGGAGCTTGAAGAAGCTACAAATGGATTCCATGAAGAACTAGGAAAGGGTGCTTTTGGAGTTGTTTACAAAGGGATAATAAAAATTGGTTCAGATGTCCAAGTCGCAGTTAAGAAGCTCAACAGTGTGATGCAAGACTCTGTGATACAAGAAGGTGAGAAAGAATTCAAGACAGAACTGAAAATTATTGGACAGACACATCACAAGAATCTAGTTCACCTGGTTGGATATTGTGATGAGGGACAAGAGCGATTACTAGTAAACCCAGTTGGAGGCAACGTACGTATGGAAATTGCTTATGGTGTTGCGAAAGGGCTTCTGTATTTGCATGAAGAGTGTACCACCCAAATCATCCACTGTGATATAAAGCCTCAGAACATACTTCTGGATGATCATTACAATGCTCGGATCTCTGATTTTGGCTTGTCGAAACTTCTGATGATGAATCAGAGCCAGACATATACTGCCATTAGAGGAACTAAAGGGTATGTTGCACCCGAGTGGTTTAGGAACATGGCTATCACTACCAAAGTTGATGTCTATAGCTTTGGTGTTGTGCTGCTGGAGATCATTTGCTGCAGGAAAAGTGTTGATATGGATAATACCAGCGAAGAGAAAGCAATTTTAACCGACTGGGTTTATAATTGTTACCAAGACGGACTTTTAGATGTGGTTCTAAATCACCAAGTCAATGCCTTGGATGACAAGACAGAGCTGGAAAAGACTGTGATGATTGCTCTTTGGTGTATCCAAGAAGACCCATTTCTTCGACCCACGATGAGAAAGGTTGTGCAGATGCTTGAAGGAGTAGTGGAAGTACATGTTCCACCATGTCCATCCCCATATTCATCAAGAACAAGCTGA

Protein Analysis

804

Amino Acids

89.39

Weight (kDa)

6.59

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 73 - 160 5.3e-14 D-mannose binding lectin
PK_Tyr_Ser-Thr PF07714 527 - 605 1.5e-11 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 528 - 782 2.4e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 612 - 784 3.8e-22 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000130)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g43780
malus_domestica MD03G1019500.v1.1 MD03G1019800.v1.1 MD03G1019900.v1.1 MD03G1020000.v1.1 MD03G1020200.v1.1 MD03G1020300.v1.1 MD05G1226500.v1.1 MD05G1226600.v1.1 MD05G1226700.v1.1 MD05G1226800.v1.1 MD09G1060200.v1.1 MD11G1021100.v1.1 MD11G1021300.v1.1 MD11G1021400.v1.1 MD11G1021500.v1.1 MD11G1024600.v1.1 MD11G1026700.v1.1
prunus_persica Prupe.6G016300_v2.0.a1 Prupe.6G016600_v2.0.a1 Prupe.6G016700_v2.0.a1 Prupe.6G017000_v2.0.a1 Prupe.6G017100_v2.0.a1 Prupe.6G017200_v2.0.a1 Prupe.6G017300_v2.0.a1 Prupe.6G017400_v2.0.a1 Prupe.6G017500_v2.0.a1
pyrus_communis pycom02g26630 pycom03g01670 pycom03g01710 pycom03g01720 pycom05g20850 pycom11g01710 pycom11g01730 pycom11g01740 pycom11g01960
rosa_chinensis RchiOBHm_Chr1g0313471 RchiOBHm_Chr1g0313491 RchiOBHm_Chr1g0313521 RchiOBHm_Chr1g0329041 RchiOBHm_Chr5g0077581 RchiOBHm_Chr5g0077681 RchiOBHm_Chr5g0077751 RchiOBHm_Chr5g0077771 RchiOBHm_Chr5g0078381 RchiOBHm_Chr5g0078421 RchiOBHm_Chr5g0078481 RchiOBHm_Chr5g0078491 RchiOBHm_Chr5g0078511 RchiOBHm_Chr5g0078561 RchiOBHm_Chr5g0078581 RchiOBHm_Chr5g0078621 RchiOBHm_Chr5g0078631 RchiOBHm_Chr5g0078641 RchiOBHm_Chr5g0078681 RchiOBHm_Chr5g0078741 RchiOBHm_Chr5g0078781 RchiOBHm_Chr5g0078791 RchiOBHm_Chr5g0078821 RchiOBHm_Chr5g0078851 RchiOBHm_Chr5g0078861 RchiOBHm_Chr5g0078881 RchiOBHm_Chr5g0078911 RchiOBHm_Chr5g0078921 RchiOBHm_Chr5g0078931 RchiOBHm_Chr5g0078951 RchiOBHm_Chr5g0079061 RchiOBHm_Chr5g0079071 RchiOBHm_Chr5g0079101 RchiOBHm_Chr5g0079111 RchiOBHm_Chr5g0079131 RchiOBHm_Chr5g0079151 RchiOBHm_Chr5g0079161 RchiOBHm_Chr5g0079211 RchiOBHm_Chr5g0079241 RchiOBHm_Chr7g0223671
rosa_laevigata RLG00000001266 RLG00000036708 RLG00000036719 RLG00000036772 RLG00000036773 RLG00000036776 RLG00000036780 RLG00000036781 RLG00000036783 RLG00000036788 RLG00000036790 RLG00000036792 RLG00000036794 RLG00000036797 RLG00000036798 RLG00000036800 RLG00000036801 RLG00000036802 RLG00000036803 RLG00000036805 RLG00000036807 RLG00000036808
rosa_multiflora Rmu_co7998958.1_g000001 Rmu_co8175864.1_g000001 Rmu_co8214884.1_g000001 Rmu_co8225446.1_g000001 Rmu_sc0000607.1_g000011 Rmu_sc0000607.1_g000012 Rmu_sc0000631.1_g000001 Rmu_sc0000631.1_g000003 Rmu_sc0000631.1_g000010 Rmu_sc0000631.1_g000012 Rmu_sc0000631.1_g000020 Rmu_sc0001347.1_g000014 Rmu_sc0002187.1_g000011 Rmu_sc0002187.1_g000014 Rmu_sc0002187.1_g000024 Rmu_sc0002187.1_g000025 Rmu_sc0003413.1_g000051 Rmu_sc0004140.1_g000002 Rmu_sc0004140.1_g000003 Rmu_sc0004140.1_g000015 Rmu_sc0004140.1_g000026 Rmu_sc0004140.1_g000036 Rmu_sc0004140.1_g000042 Rmu_sc0004140.1_g000046 Rmu_sc0004140.1_g000048 Rmu_sc0004277.1_g000002 Rmu_sc0008700.1_g000002 Rmu_sc0011280.1_g000001 Rmu_sc0011280.1_g000002 Rmu_sc0011280.1_g000009 Rmu_sc0012487.1_g000003 Rmu_sc0012487.1_g000007 Rmu_sc0012487.1_g000009 Rmu_sc0012931.1_g000001 Rmu_sc0012931.1_g000002 Rmu_sc0012931.1_g000009 Rmu_sc0015122.1_g000002 Rmu_sc0022488.1_g000001 Rmu_ssc0000425.1_g000018
rosa_roxburghii Rroxscaffold_164G00436380 Rroxscaffold_1G00003360 Rroxscaffold_1G00003370 Rroxscaffold_1G00003660 Rroxscaffold_1G00003790 Rroxscaffold_1G00003840 Rroxscaffold_1G00003950 Rroxscaffold_1G00004380 Rroxscaffold_4G00332170
rosa_rugosa Rorug05G0455800 Rorug05G0456500 Rorug05G0460800 Rorug05G0461000 Rorug05G0461200 Rorug05G0461200 Rorug05G0461300 Rorug05G0461600 Rorug05G0461700 Rorug05G0461900 Rorug05G0462000 Rorug05G0462400 Rorug05G0462500 Rorug05G0462800 Rorug05G0463200.1 Rorug05G0463300
rosa_samantha Rh1CG000600 Rh1CG008300 Rh1CG360600 Rh5AG509700 Rh5AG509800 Rh5AG510700 Rh5AG515000 Rh5AG515600 Rh5BG531300 Rh5BG531400 Rh5BG533600 Rh5BG538200 Rh5BG538300 Rh5BG538400 Rh5BG538500 Rh5BG539100 Rh5BG539200 Rh5BG539400 Rh5DG545100 Rh5DG549000 Rh5DG549200 Rh5DG549500 Rh5DG549600 Rh5DG549800 Rh5DG549900 Rh5DG550000 Rh5DG550200 Rh5DG550300 Rh7AG436900 Rh7CG456800
rosa_wichuraiana Rw0G003110 Rw0G003120 Rw0G005240 Rw0G023800 Rw0G023830 Rw1G007840 Rw5G000030 Rw5G047760 Rw5G047780 Rw5G047810 Rw5G047900 Rw5G047920 Rw5G047970 Rw5G047980 Rw5G048000 Rw5G048010 Rw5G048030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2193
Acc16I TGCGCA 1 cut(s) 1512
Acc36I ACCTGC 1 cut(s) 95
AccI GTMKAC 1 cut(s) 637
AccII CGCG 1 cut(s) 1132
AccIII TCCGGA 4 cut(s) 524, 1119, 1360, 1485
AciI CCGC 5 cut(s) 836, 1083, 1105, 1127, 1130
AclI AACGTT 1 cut(s) 718
AclWI GGATC 3 cut(s) 268, 953, 1967
AcsI RAATTY 5 cut(s) 505, 865, 1328, 1470, 1715
AcuI CTGAAG 1 cut(s) 1329
AfaI GTAC 6 cut(s) 230, 745, 1395, 1833, 1891, 2370
AfiI CCNNNNNNNGG 2 cut(s) 1104, 1823
AflIII ACRYGT 2 cut(s) 1495, 2371
AgsI TTSAA 8 cut(s) 164, 439, 551, 597, 652, 1562, 1720, 2354
AhlI ACTAGT 1 cut(s) 1807
AjnI CCWGG 3 cut(s) 622, 874, 1770
AleI CACNNNNGTG 1 cut(s) 2050
Alw21I GWGCWC 1 cut(s) 1421
Alw26I GTCTC 3 cut(s) 425, 506, 1169
AlwI GGATC 3 cut(s) 268, 953, 1967
AlwNI CAGNNNCTG 2 cut(s) 515, 900
Ama87I CYCGRG 2 cut(s) 464, 2048
Aor13HI TCCGGA 4 cut(s) 524, 1119, 1360, 1485
AoxI GGCC 2 cut(s) 920, 1173
ApeKI GCWGC 7 cut(s) 25, 101, 818, 1448, 1451, 2110, 2127
ApoI RAATTY 5 cut(s) 505, 865, 1328, 1470, 1715
ArsI GACNNNNNNTTYG 4 cut(s) 128, 160, 2073, 2105
Asp700I GAANNNNTTC 1 cut(s) 1715
AspLEI GCGC 2 cut(s) 534, 1513
AspS9I GGNCC 2 cut(s) 872, 1173
AsuC2I CCSGG 3 cut(s) 465, 466, 1099
AsuHPI GGTGA 5 cut(s) 146, 326, 1718, 1760, 2225
AvaI CYCGRG 2 cut(s) 464, 2048
AvaII GGWCC 1 cut(s) 872
AxyI CCTNAGG 1 cut(s) 255
BaeI ACNNNNGTAYC 4 cut(s) 880, 913, 1385, 1418
BbsI GAAGAC 3 cut(s) 489, 1302, 2313
Bbv12I GWGCWC 1 cut(s) 1421
BbvI GCAGC 7 cut(s) 12, 113, 805, 1435, 1438, 2097, 2114
BccI CCATC 5 cut(s) 136, 551, 755, 931, 2395
BceAI ACGGC 1 cut(s) 1261
BciT130I CCWGG 3 cut(s) 624, 876, 1772
BciVI GTATCC 4 cut(s) 235, 938, 1116, 2309
BclI TGATCA 1 cut(s) 1942
BcnI CCSGG 3 cut(s) 465, 466, 1099
BcoDI GTCTC 3 cut(s) 425, 506, 1169
BcuI ACTAGT 1 cut(s) 1807
BfaI CTAG 6 cut(s) 447, 542, 1389, 1592, 1763, 1808
BfmI CTRYAG 5 cut(s) 99, 219, 1452, 2092, 2128
BfoI RGCGCY 1 cut(s) 535
BfuAI ACCTGC 1 cut(s) 95
BfuI GTATCC 4 cut(s) 235, 938, 1116, 2309
BglII AGATCT 1 cut(s) 1033
BisI GCNGC 9 cut(s) 26, 102, 819, 836, 1130, 1449, 1452, 2111, 2128
BlsI GCNGC 9 cut(s) 27, 103, 820, 837, 1131, 1450, 1453, 2112, 2129
Bme1390I CCNGG 6 cut(s) 465, 466, 624, 876, 1099, 1772
Bme18I GGWCC 1 cut(s) 872
BmeT110I CYCGRG 2 cut(s) 464, 2048
BmgT120I GGNCC 2 cut(s) 872, 1173
BmiI GGNNCC 1 cut(s) 873
BmrFI CCNGG 6 cut(s) 465, 466, 624, 876, 1099, 1772
BmrI ACTGGG 3 cut(s) 621, 1811, 2194
BmsI GCATC 6 cut(s) 373, 958, 1078, 1147, 1671, 2337
BmuI ACTGGG 3 cut(s) 621, 1811, 2194
BpiI GAAGAC 3 cut(s) 489, 1302, 2313
BpmI CTGGAG 1 cut(s) 2135
BpuEI CTTGAG 1 cut(s) 294
BpuMI CCSGG 3 cut(s) 465, 466, 1099
BsaAI YACGTR 1 cut(s) 1835
BsaBI GATNNNNATC 1 cut(s) 561
BsaJI CCNNGG 2 cut(s) 464, 2247
BsaWI WCCGGW 4 cut(s) 524, 1119, 1360, 1485
BsaXI ACNNNNNCTCC 2 cut(s) 1815, 1845
Bsc4I CCNNNNNNNGG 2 cut(s) 1104, 1823
Bse118I RCCGGY 1 cut(s) 386
Bse1I ACTGG 4 cut(s) 616, 648, 1817, 2189
Bse21I CCTNAGG 1 cut(s) 255
Bse3DI GCAATG 3 cut(s) 12, 811, 1255
Bse8I GATNNNNATC 1 cut(s) 561
BseAI TCCGGA 4 cut(s) 524, 1119, 1360, 1485
BseBI CCWGG 3 cut(s) 624, 876, 1772
BseDI CCNNGG 2 cut(s) 464, 2247
BseGI GGATG 8 cut(s) 329, 923, 1198, 1369, 1902, 1945, 2257, 2387
BseJI GATNNNNATC 1 cut(s) 561
BseLI CCNNNNNNNGG 2 cut(s) 1104, 1823
BseMI GCAATG 3 cut(s) 12, 811, 1255
BseMII CTCAG 4 cut(s) 720, 766, 1406, 1937
BseNI ACTGG 4 cut(s) 616, 648, 1817, 2189
BseRI GAGGAG 2 cut(s) 103, 498
BseXI GCAGC 7 cut(s) 12, 113, 805, 1435, 1438, 2097, 2114
BseYI CCCAGC 1 cut(s) 813
BsgI GTGCAG 3 cut(s) 234, 886, 2363
Bsh1236I CGCG 1 cut(s) 1132
BshFI GGCC 2 cut(s) 922, 1175
BsiHKAI GWGCWC 1 cut(s) 1421
BsiHKCI CYCGRG 2 cut(s) 464, 2048
BsiSI CCGG 7 cut(s) 387, 465, 525, 1099, 1120, 1361, 1486
BsiWI CGTACG 1 cut(s) 1831
BslFI GGGAC 4 cut(s) 628, 743, 1181, 1806
BslI CCNNNNNNNGG 2 cut(s) 1104, 1823
BsmAI GTCTC 3 cut(s) 425, 506, 1169
BsmBI CGTCTC 1 cut(s) 425
BsmFI GGGAC 4 cut(s) 628, 743, 1181, 1806
BsmI GAATGC 1 cut(s) 938
BsnI GGCC 2 cut(s) 922, 1175
BsoBI CYCGRG 2 cut(s) 464, 2048
Bsp1286I GDGCHC 1 cut(s) 1421
Bsp13I TCCGGA 4 cut(s) 524, 1119, 1360, 1485
Bsp143I GATC 6 cut(s) 260, 958, 1033, 1942, 1959, 2118
BspACI CCGC 5 cut(s) 836, 1083, 1105, 1127, 1130
BspANI GGCC 2 cut(s) 922, 1175
BspCNI CTCAG 4 cut(s) 719, 765, 1407, 1936
BspEI TCCGGA 4 cut(s) 524, 1119, 1360, 1485
BspFNI CGCG 1 cut(s) 1132
BspHI TCATGA 1 cut(s) 375
BspLI GGNNCC 1 cut(s) 873
BspMAI CTGCAG 3 cut(s) 103, 1456, 2132
BspMI ACCTGC 1 cut(s) 95
BspPI GGATC 3 cut(s) 268, 953, 1967
BspQI GCTCTTC 1 cut(s) 1370
BsrDI GCAATG 3 cut(s) 12, 811, 1255
BsrFI RCCGGY 1 cut(s) 386
BsrI ACTGG 4 cut(s) 616, 648, 1817, 2189
BssAI RCCGGY 1 cut(s) 386
BssECI CCNNGG 2 cut(s) 464, 2247
BssMI GATC 6 cut(s) 260, 958, 1033, 1942, 1959, 2118
BssT1I CCWWGG 1 cut(s) 2247
Bst2UI CCWGG 3 cut(s) 624, 876, 1772
Bst4CI ACNGT 9 cut(s) 268, 287, 495, 619, 896, 983, 1676, 1910, 2278
Bst6I CTCTTC 4 cut(s) 1285, 1370, 1878, 2157
BstAPI GCANNNNNTGC 1 cut(s) 86
BstBAI YACGTR 1 cut(s) 1835
BstC8I GCNNGC 2 cut(s) 57, 249
BstDEI CTNAG 7 cut(s) 255, 579, 706, 752, 1019, 1415, 1923
BstF5I GGATG 8 cut(s) 329, 923, 1198, 1369, 1902, 1945, 2257, 2387
BstFNI CGCG 1 cut(s) 1132
BstH2I RGCGCY 1 cut(s) 535
BstHHI GCGC 2 cut(s) 534, 1513
BstKTI GATC 6 cut(s) 263, 961, 1036, 1945, 1962, 2121
BstMAI GTCTC 3 cut(s) 425, 506, 1169
BstMBI GATC 6 cut(s) 260, 958, 1033, 1942, 1959, 2118
BstMWI GCNNNNNNNGC 5 cut(s) 31, 86, 416, 538, 810
BstNI CCWGG 3 cut(s) 624, 876, 1772
BstNSI RCATGY 3 cut(s) 1164, 1499, 2375
BstSCI CCNGG 6 cut(s) 463, 464, 622, 874, 1097, 1770
BstSFI CTRYAG 5 cut(s) 99, 219, 1452, 2092, 2128
BstSNI TACGTA 1 cut(s) 1835
BstUI CGCG 1 cut(s) 1132
BstV1I GCAGC 7 cut(s) 12, 113, 805, 1435, 1438, 2097, 2114
BstV2I GAAGAC 3 cut(s) 489, 1302, 2313
BstX2I RGATCY 2 cut(s) 1033, 1959
BstYI RGATCY 2 cut(s) 1033, 1959
Bsu36I CCTNAGG 1 cut(s) 255
BsuI GTATCC 4 cut(s) 235, 938, 1116, 2309
BsuRI GGCC 2 cut(s) 922, 1175
BtsCI GGATG 8 cut(s) 329, 923, 1198, 1369, 1902, 1945, 2257, 2387
BtsI GCAGTG 1 cut(s) 573
BtsIMutI CAGTG 6 cut(s) 273, 292, 573, 609, 1681, 1906
BveI ACCTGC 1 cut(s) 95
Cac8I GCNNGC 2 cut(s) 57, 249
CaiI CAGNNNCTG 2 cut(s) 515, 900
CciI TCATGA 1 cut(s) 375
CfoI GCGC 2 cut(s) 534, 1513
Cfr10I RCCGGY 1 cut(s) 386
Cfr13I GGNCC 2 cut(s) 872, 1173
Cfr9I CCCGGG 1 cut(s) 464
CsiI ACCWGGT 1 cut(s) 1770
Csp6I GTAC 6 cut(s) 229, 744, 1394, 1832, 1890, 2369
CviQI GTAC 6 cut(s) 229, 744, 1394, 1832, 1890, 2369
DdeI CTNAG 7 cut(s) 255, 579, 706, 752, 1019, 1415, 1923
DpnI GATC 6 cut(s) 262, 960, 1035, 1944, 1961, 2120
DpnII GATC 6 cut(s) 260, 958, 1033, 1942, 1959, 2118
Eam1104I CTCTTC 4 cut(s) 1285, 1370, 1878, 2157
EarI CTCTTC 4 cut(s) 1285, 1370, 1878, 2157
EciI GGCGGA 2 cut(s) 1098, 1120
Eco105I TACGTA 1 cut(s) 1835
Eco130I CCWWGG 1 cut(s) 2247
Eco47I GGWCC 1 cut(s) 872
Eco57I CTGAAG 1 cut(s) 1329
Eco81I CCTNAGG 1 cut(s) 255
Eco88I CYCGRG 2 cut(s) 464, 2048
EcoO109I RGGNCCY 1 cut(s) 872
EcoRI GAATTC 2 cut(s) 865, 1715
EcoRII CCWGG 3 cut(s) 622, 874, 1770
EcoT14I CCWWGG 1 cut(s) 2247
EcoT22I ATGCAT 2 cut(s) 973, 1162
ErhI CCWWGG 1 cut(s) 2247
Esp3I CGTCTC 1 cut(s) 425
FalI AAGNNNNNCTT 4 cut(s) 1002, 1034, 2196, 2228
FaqI GGGAC 4 cut(s) 628, 743, 1181, 1806
FauNDI CATATG 2 cut(s) 191, 1285
FbaI TGATCA 1 cut(s) 1942
FblI GTMKAC 1 cut(s) 637
Fnu4HI GCNGC 9 cut(s) 26, 102, 819, 836, 1130, 1449, 1452, 2111, 2128
FokI GGATG 8 cut(s) 316, 910, 1205, 1376, 1889, 1952, 2264, 2374
Fsp4HI GCNGC 9 cut(s) 26, 102, 819, 836, 1130, 1449, 1452, 2111, 2128
FspBI CTAG 6 cut(s) 447, 542, 1389, 1592, 1763, 1808
FspI TGCGCA 1 cut(s) 1512
GlaI GCGC 2 cut(s) 533, 1512
GluI GCNGC 9 cut(s) 26, 102, 819, 836, 1130, 1449, 1452, 2111, 2128
GsaI CCCAGC 1 cut(s) 817
GsuI CTGGAG 1 cut(s) 2135
HaeII RGCGCY 1 cut(s) 535
HaeIII GGCC 2 cut(s) 922, 1175
HapII CCGG 7 cut(s) 387, 465, 525, 1099, 1120, 1361, 1486
HhaI GCGC 2 cut(s) 534, 1513
Hin6I GCGC 2 cut(s) 532, 1511
HinP1I GCGC 2 cut(s) 532, 1511
HinfI GANTC 8 cut(s) 442, 562, 656, 992, 1578, 1688, 1759, 1996
HpaII CCGG 7 cut(s) 387, 465, 525, 1099, 1120, 1361, 1486
HphI GGTGA 5 cut(s) 146, 326, 1718, 1760, 2225
Hpy166II GTNNAC 8 cut(s) 271, 573, 638, 1243, 1618, 1768, 1813, 1890
Hpy8I GTNNAC 8 cut(s) 271, 573, 638, 1243, 1618, 1768, 1813, 1890
HpyAV CCTTC 5 cut(s) 626, 1330, 1546, 1697, 2348
HpyCH4III ACNGT 9 cut(s) 268, 287, 495, 619, 896, 983, 1676, 1910, 2278
HpyCH4IV ACGT 5 cut(s) 434, 718, 1396, 1830, 1834
HpyF10VI GCNNNNNNNGC 5 cut(s) 31, 86, 416, 538, 810
HpyF3I CTNAG 7 cut(s) 255, 579, 706, 752, 1019, 1415, 1923
HpySE526I ACGT 5 cut(s) 434, 718, 1396, 1830, 1834
HspAI GCGC 2 cut(s) 532, 1511
Kpn2I TCCGGA 4 cut(s) 524, 1119, 1360, 1485
Ksp22I TGATCA 1 cut(s) 1942
Kzo9I GATC 6 cut(s) 260, 958, 1033, 1942, 1959, 2118
LguI GCTCTTC 1 cut(s) 1370
LmnI GCTCC 1 cut(s) 1555
Lsp1109I GCAGC 7 cut(s) 12, 113, 805, 1435, 1438, 2097, 2114
LweI GCATC 6 cut(s) 373, 958, 1078, 1147, 1671, 2337
MabI ACCWGGT 1 cut(s) 1770
MaeI CTAG 6 cut(s) 447, 542, 1389, 1592, 1763, 1808
MaeII ACGT 5 cut(s) 434, 718, 1396, 1830, 1834
MaeIII GTNAC 5 cut(s) 107, 134, 389, 887, 2198
MalI GATC 6 cut(s) 262, 960, 1035, 1944, 1961, 2120
MboI GATC 6 cut(s) 260, 958, 1033, 1942, 1959, 2118
MflI RGATCY 2 cut(s) 1033, 1959
MhlI GDGCHC 1 cut(s) 1421
MlyI GAGTC 2 cut(s) 665, 1682
MmeI TCCRAC 3 cut(s) 1241, 1756, 1801
Mph1103I ATGCAT 2 cut(s) 973, 1162
MroI TCCGGA 4 cut(s) 524, 1119, 1360, 1485
MroXI GAANNNNTTC 1 cut(s) 1715
MseI TTAA 4 cut(s) 858, 1308, 1662, 2177
MslI CAYNNNNRTG 2 cut(s) 1950, 2050
MspI CCGG 7 cut(s) 387, 465, 525, 1099, 1120, 1361, 1486
MspR9I CCNGG 6 cut(s) 465, 466, 624, 876, 1099, 1772
Mva1269I GAATGC 1 cut(s) 938
MvaI CCWGG 3 cut(s) 624, 876, 1772
MvnI CGCG 1 cut(s) 1132
MwoI GCNNNNNNNGC 5 cut(s) 31, 86, 416, 538, 810
NciI CCSGG 3 cut(s) 465, 466, 1099
NdeI CATATG 2 cut(s) 191, 1285
NdeII GATC 6 cut(s) 260, 958, 1033, 1942, 1959, 2118
NlaIV GGNNCC 1 cut(s) 873
NmuCI GTSAC 1 cut(s) 134
NsbI TGCGCA 1 cut(s) 1512
NsiI ATGCAT 2 cut(s) 973, 1162
NspI RCATGY 3 cut(s) 1164, 1499, 2375
OliI CACNNNNGTG 1 cut(s) 2050
PagI TCATGA 1 cut(s) 375
PciI ACATGT 2 cut(s) 1495, 2371
PciSI GCTCTTC 1 cut(s) 1370
PctI GAATGC 1 cut(s) 938
PdmI GAANNNNTTC 1 cut(s) 1715
PfeI GAWTC 6 cut(s) 442, 562, 992, 1578, 1759, 1996
Pfl23II CGTACG 1 cut(s) 1831
PkrI GCNGC 9 cut(s) 27, 103, 820, 837, 1131, 1450, 1453, 2112, 2129
PleI GAGTC 2 cut(s) 664, 1682
PpsI GAGTC 2 cut(s) 664, 1682
Ppu21I YACGTR 1 cut(s) 1835
PpuMI RGGWCCY 1 cut(s) 872
PscI ACATGT 2 cut(s) 1495, 2371
PsiI TTATAA 1 cut(s) 2193
Psp1406I AACGTT 1 cut(s) 718
Psp5II RGGWCCY 1 cut(s) 872
Psp6I CCWGG 3 cut(s) 622, 874, 1770
PspFI CCCAGC 1 cut(s) 813
PspGI CCWGG 3 cut(s) 622, 874, 1770
PspLI CGTACG 1 cut(s) 1831
PspN4I GGNNCC 1 cut(s) 873
PspPI GGNCC 2 cut(s) 872, 1173
PspPPI RGGWCCY 1 cut(s) 872
PstI CTGCAG 3 cut(s) 103, 1456, 2132
PstNI CAGNNNCTG 2 cut(s) 515, 900
PsuI RGATCY 2 cut(s) 1033, 1959
RsaI GTAC 6 cut(s) 230, 745, 1395, 1833, 1891, 2370
RsaNI GTAC 6 cut(s) 229, 744, 1394, 1832, 1890, 2369
RseI CAYNNNNRTG 2 cut(s) 1950, 2050
SapI GCTCTTC 1 cut(s) 1370
SaqAI TTAA 4 cut(s) 858, 1308, 1662, 2177
SatI GCNGC 9 cut(s) 26, 102, 819, 836, 1130, 1449, 1452, 2111, 2128
Sau3AI GATC 6 cut(s) 260, 958, 1033, 1942, 1959, 2118
Sau96I GGNCC 2 cut(s) 872, 1173
SchI GAGTC 2 cut(s) 665, 1682
ScrFI CCNGG 6 cut(s) 465, 466, 624, 876, 1099, 1772
SduI GDGCHC 1 cut(s) 1421
SexAI ACCWGGT 1 cut(s) 1770
SfaNI GCATC 6 cut(s) 373, 958, 1078, 1147, 1671, 2337
SfcI CTRYAG 5 cut(s) 99, 219, 1452, 2092, 2128
SinI GGWCC 1 cut(s) 872
SmaI CCCGGG 1 cut(s) 466
SmiMI CAYNNNNRTG 2 cut(s) 1950, 2050
SmlI CTYRAG 1 cut(s) 309
SmoI CTYRAG 1 cut(s) 309
SnaBI TACGTA 1 cut(s) 1835
SpeI ACTAGT 1 cut(s) 1807
SrfI GCCCGGGC 1 cut(s) 466
SsiI CCGC 5 cut(s) 836, 1083, 1105, 1127, 1130
SspI AATATT 2 cut(s) 779, 844
SspMI CTAG 6 cut(s) 447, 542, 1389, 1592, 1763, 1808
StyD4I CCNGG 6 cut(s) 463, 464, 622, 874, 1097, 1770
StyI CCWWGG 1 cut(s) 2247
TaaI ACNGT 9 cut(s) 268, 287, 495, 619, 896, 983, 1676, 1910, 2278
TaiI ACGT 5 cut(s) 437, 721, 1399, 1833, 1837
TaqI TCGA 3 cut(s) 502, 1979, 2320
TatI WGTACW 1 cut(s) 2368
TauI GCSGC 2 cut(s) 838, 1132
TfiI GAWTC 6 cut(s) 442, 562, 992, 1578, 1759, 1996
Tru1I TTAA 4 cut(s) 858, 1308, 1662, 2177
Tru9I TTAA 4 cut(s) 858, 1308, 1662, 2177
TscAI CASTG 6 cut(s) 273, 292, 580, 616, 1681, 1913
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 7 cut(s) 25, 101, 818, 1448, 1451, 2110, 2127
Tsp45I GTSAC 1 cut(s) 134
TspGWI ACGGA 2 cut(s) 731, 2223
TspMI CCCGGG 1 cut(s) 464
TspRI CASTG 6 cut(s) 273, 292, 580, 616, 1681, 1913
VpaK11BI GGWCC 1 cut(s) 872
XapI RAATTY 5 cut(s) 505, 865, 1328, 1470, 1715
XceI RCATGY 3 cut(s) 1164, 1499, 2375
XmaI CCCGGG 1 cut(s) 464
XmiI GTMKAC 1 cut(s) 637
XmnI GAANNNNTTC 1 cut(s) 1715
XspI CTAG 6 cut(s) 447, 542, 1389, 1592, 1763, 1808
Zsp2I ATGCAT 2 cut(s) 973, 1162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.