RLG00000036800

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000130
Physical Location & Seq
Reverse (-)
84731409 .. 84736229
4821 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036800

Sequence Viewer

Length: 2538 bp
ATGTTATGGGGGACGTTCAAGAATCCTACCGATACCATGTTGCCTGGGCAAATATTGGAAAGAGGAGGGAAGCTTTCTTTTCGAAATTTAGAGACTGACTATTTGAGTGGACACTTCCAGCTAGATTTTCAAGATGATGGGAATCTTGTGCTTTGTGGGCAAAGACCTTTTTATTCAACCGAGACTACCGCAGGGAGTATTTCAGGTAGTGATGAAGGTCTACAGTTGGTTTTCAATGACTCAGGTAGCTTCTATATTCTGCGAGTGAATGGTGGATATTATACTTTCACAGCGGAGGAACGTGCAGCAAGGGACTACTATCTCCGGACAACTGTCAGCTTTGATGGGGTTTTGGCTCAATATTTCCTCCCAAAGACTTCTGTTGGCAACATAAACTGGGTTCCTCTTTGGTCAGAGCCATCAAATATTTGCCAAAAAATTAATGCTGATTCAGGTCCTAGTATTTGTGGGTACAACAGTATCTGCACTTACAAAGCAGACAAGAGTCCCACCTGTGAATGCCCGACAGGATACTCTTTACTTGATCCAAATGATCCATACGGGAGCTGCTATCCAGCTTTCAAACAAGGCTGTGAAAATGAGCGTAGTTATGCACAAGATATGTACGATGTTCAGGTGCTAGAAAATACTGATTGGCTTATCTCCAGTGCTGCAGTTTGTCTGGGACTTTTCTTCAGTTTCCGGAAGAAACATGCAAGGTTTCCACAAAATGACTTGGACACAAATTTGCACTCTTTTAGTCACAAAAAGCTCGAAGACGCAACAAATGGATTCAATGAAGAATTAGGGAAGGGTTCTTTTGGAGTTGTTTACAAAGGGATAATACAAATTGGTTCGGGCGTCCAAGTGCCAGTGAAGAAGCTCAACTGTTGGATACAAGATGGTGAGAAGGAATTCAAGACAGAACTGAACATAATTGGTCGGACACATCACAAGAATCTGGTTCACCTGGTTGGATATTGTGATGAGGGACAACAGCGATCACTTCCTCAGAACATTCTTCTTGATGATAATTACATTGCTCGGATCTCTGATTTCGGCTTGGCGAAACTTTTGATGACGAATCAGAGCAAGACACATACTGCCATTAGAGGAACAAAAGGTTATGTTGCACCTGAGTGGTTTAGGAACATGGCAATCACTACCAAAGTTGATGTGTATAGCTTTGGTATGGTGCTGCTAGAGATCATTTGTTGTAGGAGAAGTGTTGATATGGATAATGATTACGAAGAAAAAGCAATTTTAACGGATTGGGTTTATGATTGCTACCGAGAAGGATTATTGGAGGCTATTCTGGATCACAAAGTTGATGCCTTGGATTATGAAATGAAGCTAGAAGAGACTGTCATGATTGCTCTTTGGTGTATTCAAGAAGACCCATCTCTTCGACCCACAATGAGGAAGGTTGTGCAGATGCTTGAAGGAGTAGTGGAAGTACATGTTCCACCCAATCAAACCTGTCAGAAAAAGAAGTTTCCGCTCTCATATGGGAGAGAAGATAACAACTTGGATGCAACAACCTTCATCAAATTGATGACAGTTAAGAAGAAGAAGAGCGGGGCCACTTCAGTTCATGTGGGATCAGTCCTTCTGGGCACCTCTGTCTTTGTTAGTTTTATGTTAGATGCTGCAGTTTGTCCGGGACTGTTCTTTGGTTTGAGGAAGAAACGTGTAGGTCACAAAGAGCTCGAAGAAGCAACAAATGGATTCAACGAAGAATTAGGGAAGGGTTCTTTTGGAATTGTTTACAAAGGGATAATACAAATTGGTTCGGGTGTCCAAGTGGCAGTGAAGAAGCTCAACCGTGTGATACAAGATGGTGAGAAGGAATTCAAGACGGAACTGAACATAATTGGTCGAACACATCACAAGAATCTGGTTCACCTGGTTGGATATTGTGATGAGGGACAACAGCGATCACTAGTATATGAATTCTTGAGCAATGGAACATTAGCAAGCTTCCTTTTTGGTGATGAGAAACCCAGTTGGAGGCAACGAATGGAAATTGCTTATGGTGTTGCCAAAGGGCTTCTGTATTTGCATAAAGAGTGTACCACGCAAACCATCCATTGTGATATAAAGCCTCAGAACATCCTTCTTGATGATAATTACATTGGTTGGATCTTTGATTTCGGCTTGGCGAAACTTTTGATGACGAATCAGAGCCAGAAGCATACTGCCATTAGGGGAACAAAAGGGTATGTTGCACCTGAGTGGTTTAGGAACATGGAAATCACTACCAAAGTTGATGTGTATAGCTTTGGAATTAGAGGGAGTGTTAGAGACTGTTCTAGATCACGAGTCGACGCCTTGGAAGGTGAAATGAAGCTGGAAGAGGTTGTCATGATTGCTCTATGCTGTATTCAAGAAGACCCATCTCTTCGACCCAAGATGAGGAAGGTTGTGCAGATGCTTGAAGGATTAGTGGAAGTACATGCTCCACCTTGCCCATCCCCATACAACAGTGGCGTGAAACTTGTTTGGTTTGTGTCGTCAAAGATTTACAGCCAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin Pkinase

Protein Analysis

846

Amino Acids

95.32

Weight (kDa)

6.22

Isoelectric Point (pI)

34.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 265 - 333 6.9e-09 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 337 - 478 6e-19 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 338 - 480 9.1e-16 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 577 - 763 4.1e-38 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 578 - 763 8.8e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000130)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g43780
malus_domestica MD03G1019500.v1.1 MD03G1019800.v1.1 MD03G1019900.v1.1 MD03G1020000.v1.1 MD03G1020200.v1.1 MD03G1020300.v1.1 MD05G1226500.v1.1 MD05G1226600.v1.1 MD05G1226700.v1.1 MD05G1226800.v1.1 MD09G1060200.v1.1 MD11G1021100.v1.1 MD11G1021300.v1.1 MD11G1021400.v1.1 MD11G1021500.v1.1 MD11G1024600.v1.1 MD11G1026700.v1.1
prunus_persica Prupe.6G016300_v2.0.a1 Prupe.6G016600_v2.0.a1 Prupe.6G016700_v2.0.a1 Prupe.6G017000_v2.0.a1 Prupe.6G017100_v2.0.a1 Prupe.6G017200_v2.0.a1 Prupe.6G017300_v2.0.a1 Prupe.6G017400_v2.0.a1 Prupe.6G017500_v2.0.a1
pyrus_communis pycom02g26630 pycom03g01670 pycom03g01710 pycom03g01720 pycom05g20850 pycom11g01710 pycom11g01730 pycom11g01740 pycom11g01960
rosa_chinensis RchiOBHm_Chr1g0313471 RchiOBHm_Chr1g0313491 RchiOBHm_Chr1g0313521 RchiOBHm_Chr1g0329041 RchiOBHm_Chr5g0077581 RchiOBHm_Chr5g0077681 RchiOBHm_Chr5g0077751 RchiOBHm_Chr5g0077771 RchiOBHm_Chr5g0078381 RchiOBHm_Chr5g0078421 RchiOBHm_Chr5g0078481 RchiOBHm_Chr5g0078491 RchiOBHm_Chr5g0078511 RchiOBHm_Chr5g0078561 RchiOBHm_Chr5g0078581 RchiOBHm_Chr5g0078621 RchiOBHm_Chr5g0078631 RchiOBHm_Chr5g0078641 RchiOBHm_Chr5g0078681 RchiOBHm_Chr5g0078741 RchiOBHm_Chr5g0078781 RchiOBHm_Chr5g0078791 RchiOBHm_Chr5g0078821 RchiOBHm_Chr5g0078851 RchiOBHm_Chr5g0078861 RchiOBHm_Chr5g0078881 RchiOBHm_Chr5g0078911 RchiOBHm_Chr5g0078921 RchiOBHm_Chr5g0078931 RchiOBHm_Chr5g0078951 RchiOBHm_Chr5g0079061 RchiOBHm_Chr5g0079071 RchiOBHm_Chr5g0079101 RchiOBHm_Chr5g0079111 RchiOBHm_Chr5g0079131 RchiOBHm_Chr5g0079151 RchiOBHm_Chr5g0079161 RchiOBHm_Chr5g0079211 RchiOBHm_Chr5g0079241 RchiOBHm_Chr7g0223671
rosa_laevigata RLG00000001266 RLG00000036708 RLG00000036719 RLG00000036772 RLG00000036773 RLG00000036776 RLG00000036780 RLG00000036781 RLG00000036783 RLG00000036788 RLG00000036790 RLG00000036792 RLG00000036794 RLG00000036797 RLG00000036798 RLG00000036800 RLG00000036801 RLG00000036802 RLG00000036803 RLG00000036805 RLG00000036807 RLG00000036808
rosa_multiflora Rmu_co7998958.1_g000001 Rmu_co8175864.1_g000001 Rmu_co8214884.1_g000001 Rmu_co8225446.1_g000001 Rmu_sc0000607.1_g000011 Rmu_sc0000607.1_g000012 Rmu_sc0000631.1_g000001 Rmu_sc0000631.1_g000003 Rmu_sc0000631.1_g000010 Rmu_sc0000631.1_g000012 Rmu_sc0000631.1_g000020 Rmu_sc0001347.1_g000014 Rmu_sc0002187.1_g000011 Rmu_sc0002187.1_g000014 Rmu_sc0002187.1_g000024 Rmu_sc0002187.1_g000025 Rmu_sc0003413.1_g000051 Rmu_sc0004140.1_g000002 Rmu_sc0004140.1_g000003 Rmu_sc0004140.1_g000015 Rmu_sc0004140.1_g000026 Rmu_sc0004140.1_g000036 Rmu_sc0004140.1_g000042 Rmu_sc0004140.1_g000046 Rmu_sc0004140.1_g000048 Rmu_sc0004277.1_g000002 Rmu_sc0008700.1_g000002 Rmu_sc0011280.1_g000001 Rmu_sc0011280.1_g000002 Rmu_sc0011280.1_g000009 Rmu_sc0012487.1_g000003 Rmu_sc0012487.1_g000007 Rmu_sc0012487.1_g000009 Rmu_sc0012931.1_g000001 Rmu_sc0012931.1_g000002 Rmu_sc0012931.1_g000009 Rmu_sc0015122.1_g000002 Rmu_sc0022488.1_g000001 Rmu_ssc0000425.1_g000018
rosa_roxburghii Rroxscaffold_164G00436380 Rroxscaffold_1G00003360 Rroxscaffold_1G00003370 Rroxscaffold_1G00003660 Rroxscaffold_1G00003790 Rroxscaffold_1G00003840 Rroxscaffold_1G00003950 Rroxscaffold_1G00004380 Rroxscaffold_4G00332170
rosa_rugosa Rorug05G0455800 Rorug05G0456500 Rorug05G0460800 Rorug05G0461000 Rorug05G0461200 Rorug05G0461200 Rorug05G0461300 Rorug05G0461600 Rorug05G0461700 Rorug05G0461900 Rorug05G0462000 Rorug05G0462400 Rorug05G0462500 Rorug05G0462800 Rorug05G0463200.1 Rorug05G0463300
rosa_samantha Rh1CG000600 Rh1CG008300 Rh1CG360600 Rh5AG509700 Rh5AG509800 Rh5AG510700 Rh5AG515000 Rh5AG515600 Rh5BG531300 Rh5BG531400 Rh5BG533600 Rh5BG538200 Rh5BG538300 Rh5BG538400 Rh5BG538500 Rh5BG539100 Rh5BG539200 Rh5BG539400 Rh5DG545100 Rh5DG549000 Rh5DG549200 Rh5DG549500 Rh5DG549600 Rh5DG549800 Rh5DG549900 Rh5DG550000 Rh5DG550200 Rh5DG550300 Rh7AG436900 Rh7CG456800
rosa_wichuraiana Rw0G003110 Rw0G003120 Rw0G005240 Rw0G023800 Rw0G023830 Rw1G007840 Rw5G000030 Rw5G047760 Rw5G047780 Rw5G047810 Rw5G047900 Rw5G047920 Rw5G047970 Rw5G047980 Rw5G048000 Rw5G048010 Rw5G048030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1616
AccBSI CCGCTC 2 cut(s) 1501, 1578
AccI GTMKAC 2 cut(s) 220, 2325
AccIII TCCGGA 2 cut(s) 324, 702
AciI CCGC 4 cut(s) 189, 293, 1499, 1578
AclWI GGATC 6 cut(s) 539, 548, 1055, 1326, 1609, 2150
AcsI RAATTY 5 cut(s) 85, 745, 914, 1850, 1952
AcuI CTGAAG 2 cut(s) 679, 1572
AcyI GRCGYC 2 cut(s) 861, 2328
AfaI GTAC 6 cut(s) 473, 626, 1458, 2074, 2454, 2534
AfiI CCNNNNNNNGG 3 cut(s) 1419, 2010, 2415
AflIII ACRYGT 2 cut(s) 1459, 1690
AhlI ACTAGT 1 cut(s) 1942
AjnI CCWGG 3 cut(s) 43, 969, 1905
AleI CACNNNNGTG 2 cut(s) 1138, 2233
Alw21I GWGCWC 1 cut(s) 1710
Alw26I GTCTC 4 cut(s) 86, 176, 1355, 2298
AlwI GGATC 6 cut(s) 539, 548, 1055, 1326, 1609, 2150
AlwNI CAGNNNCTG 1 cut(s) 483
Aor13HI TCCGGA 2 cut(s) 324, 702
AoxI GGCC 1 cut(s) 1581
ApeKI GCWGC 5 cut(s) 305, 567, 671, 1198, 1649
ApoI RAATTY 5 cut(s) 85, 745, 914, 1850, 1952
ArsI GACNNNNNNTTYG 2 cut(s) 85, 117
AseI ATTAAT 1 cut(s) 441
Asp700I GAANNNNTTC 2 cut(s) 914, 1850
AspS9I GGNCC 2 cut(s) 455, 1581
AsuC2I CCSGG 1 cut(s) 1662
AsuHPI GGTGA 6 cut(s) 917, 959, 1853, 1895, 2003, 2351
AsuII TTCGAA 1 cut(s) 82
AvaII GGWCC 1 cut(s) 455
BaeGI GKGCMC 1 cut(s) 1619
BaeI ACNNNNGTAYC 4 cut(s) 463, 463, 496, 496
BanI GGYRCC 1 cut(s) 1616
BanII GRGCYC 1 cut(s) 1710
BauI CACGAG 1 cut(s) 2319
BbsI GAAGAC 3 cut(s) 783, 1401, 2397
Bbv12I GWGCWC 1 cut(s) 1710
BbvI GCAGC 5 cut(s) 317, 554, 658, 1185, 1636
BccI CCATC 9 cut(s) 131, 338, 427, 896, 1408, 1832, 2093, 2404, 2479
BciT130I CCWGG 3 cut(s) 45, 971, 1907
BciVI GTATCC 2 cut(s) 524, 888
BcnI CCSGG 1 cut(s) 1662
BcoDI GTCTC 4 cut(s) 86, 176, 1355, 2298
BcuI ACTAGT 1 cut(s) 1942
BfaI CTAG 8 cut(s) 122, 459, 641, 1202, 1355, 1943, 2313, 2536
BfmI CTRYAG 3 cut(s) 221, 672, 1650
BfuI GTATCC 2 cut(s) 524, 888
BisI GCNGC 5 cut(s) 306, 568, 672, 1199, 1650
BlsI GCNGC 5 cut(s) 307, 569, 673, 1200, 1651
BmcAI AGTACT 1 cut(s) 2534
Bme1390I CCNGG 4 cut(s) 45, 971, 1662, 1907
Bme18I GGWCC 1 cut(s) 455
BmgT120I GGNCC 2 cut(s) 455, 1581
BmiI GGNNCC 3 cut(s) 402, 1582, 1618
BmrFI CCNGG 4 cut(s) 45, 971, 1662, 1907
BmrI ACTGGG 2 cut(s) 406, 1998
BmsI GCATC 5 cut(s) 1321, 1425, 1522, 1636, 2421
BmuI ACTGGG 2 cut(s) 406, 1998
BoxI GACNNNNGTC 2 cut(s) 332, 504
BpiI GAAGAC 3 cut(s) 783, 1401, 2397
BpmI CTGGAG 1 cut(s) 649
Bpu14I TTCGAA 1 cut(s) 82
BpuEI CTTGAG 1 cut(s) 1978
BpuMI CCSGG 1 cut(s) 1662
BsaBI GATNNNNATC 1 cut(s) 141
BsaHI GRCGYC 2 cut(s) 861, 2328
BsaJI CCNNGG 3 cut(s) 44, 1335, 2331
BsaWI WCCGGW 2 cut(s) 324, 702
Bsc4I CCNNNNNNNGG 3 cut(s) 1419, 2010, 2415
Bse1I ACTGG 5 cut(s) 401, 666, 872, 2004, 2530
Bse3DI GCAATG 2 cut(s) 1038, 1969
Bse8I GATNNNNATC 1 cut(s) 141
BseAI TCCGGA 2 cut(s) 324, 702
BseBI CCWGG 3 cut(s) 45, 971, 1907
BseDI CCNNGG 3 cut(s) 44, 1335, 2331
BseGI GGATG 4 cut(s) 1537, 2085, 2112, 2471
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 3 cut(s) 1419, 2010, 2415
BseMI GCAATG 2 cut(s) 1038, 1969
BseMII CTCAG 5 cut(s) 255, 1025, 1128, 2120, 2223
BseNI ACTGG 5 cut(s) 401, 666, 872, 2004, 2530
BseRI GAGGAG 1 cut(s) 78
BseSI GKGCMC 1 cut(s) 1619
BseXI GCAGC 5 cut(s) 317, 554, 658, 1185, 1636
BsgI GTGCAG 4 cut(s) 324, 469, 1451, 2447
BshFI GGCC 1 cut(s) 1583
BshNI GGYRCC 1 cut(s) 1616
BsiHKAI GWGCWC 1 cut(s) 1710
BsiSI CCGG 3 cut(s) 325, 703, 1661
BslFI GGGAC 7 cut(s) 25, 326, 492, 699, 1005, 1677, 1941
BslI CCNNNNNNNGG 3 cut(s) 1419, 2010, 2415
BsmAI GTCTC 4 cut(s) 86, 176, 1355, 2298
BsmFI GGGAC 7 cut(s) 25, 326, 492, 699, 1005, 1677, 1941
BsmI GAATGC 1 cut(s) 524
BsnI GGCC 1 cut(s) 1583
Bsp119I TTCGAA 1 cut(s) 82
Bsp1286I GDGCHC 2 cut(s) 1619, 1710
Bsp13I TCCGGA 2 cut(s) 324, 702
BspACI CCGC 4 cut(s) 189, 293, 1499, 1578
BspANI GGCC 1 cut(s) 1583
BspCNI CTCAG 5 cut(s) 254, 1024, 1129, 2119, 2224
BspEI TCCGGA 2 cut(s) 324, 702
BspHI TCATGA 2 cut(s) 1368, 2364
BspLI GGNNCC 3 cut(s) 402, 1582, 1618
BspMAI CTGCAG 2 cut(s) 676, 1654
BspPI GGATC 6 cut(s) 539, 548, 1055, 1326, 1609, 2150
BspQI GCTCTTC 1 cut(s) 1568
BspT104I TTCGAA 1 cut(s) 82
BspT107I GGYRCC 1 cut(s) 1616
BsrBI CCGCTC 2 cut(s) 1501, 1578
BsrDI GCAATG 2 cut(s) 1038, 1969
BsrI ACTGG 5 cut(s) 401, 666, 872, 2004, 2530
BssECI CCNNGG 3 cut(s) 44, 1335, 2331
BssNI GRCGYC 2 cut(s) 861, 2328
BssSI CACGAG 1 cut(s) 2319
BssT1I CCWWGG 2 cut(s) 1335, 2331
Bst2BI CACGAG 1 cut(s) 2319
Bst2UI CCWGG 3 cut(s) 45, 971, 1907
Bst6I CTCTTC 5 cut(s) 1353, 1410, 1568, 2349, 2406
BstACI GRCGYC 2 cut(s) 861, 2328
BstBI TTCGAA 1 cut(s) 82
BstC8I GCNNGC 1 cut(s) 1978
BstDEI CTNAG 5 cut(s) 241, 1011, 1137, 2106, 2232
BstF5I GGATG 4 cut(s) 1537, 2085, 2112, 2471
BstMAI GTCTC 4 cut(s) 86, 176, 1355, 2298
BstMWI GCNNNNNNNGC 1 cut(s) 157
BstNI CCWGG 3 cut(s) 45, 971, 1907
BstNSI RCATGY 3 cut(s) 716, 1463, 2459
BstPAI GACNNNNGTC 2 cut(s) 332, 504
BstSCI CCNGG 4 cut(s) 43, 969, 1660, 1905
BstSFI CTRYAG 3 cut(s) 221, 672, 1650
BstSLI GKGCMC 1 cut(s) 1619
BstV1I GCAGC 5 cut(s) 317, 554, 658, 1185, 1636
BstV2I GAAGAC 3 cut(s) 783, 1401, 2397
BstX2I RGATCY 2 cut(s) 1047, 2142
BstYI RGATCY 2 cut(s) 1047, 2142
BsuI GTATCC 2 cut(s) 524, 888
BsuRI GGCC 1 cut(s) 1583
BtsCI GGATG 4 cut(s) 1537, 2085, 2112, 2471
BtsI GCAGTG 1 cut(s) 1815
BtsIMutI CAGTG 4 cut(s) 673, 879, 1815, 2491
Cac8I GCNNGC 1 cut(s) 1978
CaiI CAGNNNCTG 1 cut(s) 483
CciI TCATGA 2 cut(s) 1368, 2364
Cfr13I GGNCC 2 cut(s) 455, 1581
CseI GACGC 3 cut(s) 788, 850, 2336
CsiI ACCWGGT 2 cut(s) 969, 1905
Csp6I GTAC 6 cut(s) 472, 625, 1457, 2073, 2453, 2533
CviAII CATG 9 cut(s) 37, 713, 1153, 1369, 1460, 1595, 2248, 2365, 2456
CviQI GTAC 6 cut(s) 472, 625, 1457, 2073, 2453, 2533
DdeI CTNAG 5 cut(s) 241, 1011, 1137, 2106, 2232
Eam1104I CTCTTC 5 cut(s) 1353, 1410, 1568, 2349, 2406
EarI CTCTTC 5 cut(s) 1353, 1410, 1568, 2349, 2406
Ecl136II GAGCTC 1 cut(s) 1708
Eco130I CCWWGG 2 cut(s) 1335, 2331
Eco24I GRGCYC 1 cut(s) 1710
Eco47I GGWCC 1 cut(s) 455
Eco53kI GAGCTC 1 cut(s) 1708
Eco57I CTGAAG 2 cut(s) 679, 1572
EcoICRI GAGCTC 1 cut(s) 1708
EcoO109I RGGNCCY 1 cut(s) 455
EcoRI GAATTC 3 cut(s) 914, 1850, 1952
EcoRII CCWGG 3 cut(s) 43, 969, 1905
EcoT14I CCWWGG 2 cut(s) 1335, 2331
EcoT38I GRGCYC 1 cut(s) 1710
ErhI CCWWGG 2 cut(s) 1335, 2331
FaeI CATG 9 cut(s) 40, 716, 1156, 1372, 1463, 1598, 2251, 2368, 2459
FaqI GGGAC 7 cut(s) 25, 326, 492, 699, 1005, 1677, 1941
FatI CATG 9 cut(s) 36, 712, 1152, 1368, 1459, 1594, 2247, 2364, 2455
FauI CCCGC 1 cut(s) 1571
FauNDI CATATG 1 cut(s) 1507
FblI GTMKAC 2 cut(s) 220, 2325
Fnu4HI GCNGC 5 cut(s) 306, 568, 672, 1199, 1650
FokI GGATG 4 cut(s) 1544, 2072, 2099, 2458
FriOI GRGCYC 1 cut(s) 1710
Fsp4HI GCNGC 5 cut(s) 306, 568, 672, 1199, 1650
FspBI CTAG 8 cut(s) 122, 459, 641, 1202, 1355, 1943, 2313, 2536
GluI GCNGC 5 cut(s) 306, 568, 672, 1199, 1650
GsuI CTGGAG 1 cut(s) 649
HaeIII GGCC 1 cut(s) 1583
HapII CCGG 3 cut(s) 325, 703, 1661
HgaI GACGC 3 cut(s) 788, 850, 2336
Hin1I GRCGYC 2 cut(s) 861, 2328
Hin1II CATG 9 cut(s) 40, 716, 1156, 1372, 1463, 1598, 2251, 2368, 2459
HincII GTYRAC 1 cut(s) 2326
HindII GTYRAC 1 cut(s) 2326
HindIII AAGCTT 2 cut(s) 71, 1978
HpaII CCGG 3 cut(s) 325, 703, 1661
HphI GGTGA 6 cut(s) 917, 959, 1853, 1895, 2003, 2351
Hpy166II GTNNAC 8 cut(s) 110, 221, 832, 967, 1768, 1903, 2073, 2326
Hpy188I TCNGA 9 cut(s) 415, 945, 1014, 1047, 1054, 1089, 1485, 2109, 2184
Hpy8I GTNNAC 8 cut(s) 110, 221, 832, 967, 1768, 1903, 2073, 2326
Hpy99I CGWCG 1 cut(s) 2330
HpyCH4IV ACGT 3 cut(s) 14, 301, 1690
HpyF10VI GCNNNNNNNGC 1 cut(s) 157
HpyF3I CTNAG 5 cut(s) 241, 1011, 1137, 2106, 2232
HpySE526I ACGT 3 cut(s) 14, 301, 1690
Hsp92I GRCGYC 2 cut(s) 861, 2328
Hsp92II CATG 9 cut(s) 40, 716, 1156, 1372, 1463, 1598, 2251, 2368, 2459
Kpn2I TCCGGA 2 cut(s) 324, 702
LguI GCTCTTC 1 cut(s) 1568
LmnI GCTCC 2 cut(s) 564, 2464
Lsp1109I GCAGC 5 cut(s) 317, 554, 658, 1185, 1636
LweI GCATC 5 cut(s) 1321, 1425, 1522, 1636, 2421
MabI ACCWGGT 2 cut(s) 969, 1905
MaeI CTAG 8 cut(s) 122, 459, 641, 1202, 1355, 1943, 2313, 2536
MaeII ACGT 3 cut(s) 14, 301, 1690
MaeIII GTNAC 2 cut(s) 761, 1697
MbiI CCGCTC 2 cut(s) 1501, 1578
MflI RGATCY 2 cut(s) 1047, 2142
MhlI GDGCHC 2 cut(s) 1619, 1710
MlyI GAGTC 3 cut(s) 233, 514, 2331
MmeI TCCRAC 6 cut(s) 872, 923, 955, 1891, 1988, 2120
MroI TCCGGA 2 cut(s) 324, 702
MroXI GAANNNNTTC 2 cut(s) 914, 1850
MseI TTAA 3 cut(s) 441, 1265, 1563
MslI CAYNNNNRTG 2 cut(s) 1138, 2233
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 3 cut(s) 325, 703, 1661
MspR9I CCNGG 4 cut(s) 45, 971, 1662, 1907
Mva1269I GAATGC 1 cut(s) 524
MvaI CCWGG 3 cut(s) 45, 971, 1907
MwoI GCNNNNNNNGC 1 cut(s) 157
NciI CCSGG 1 cut(s) 1662
NdeI CATATG 1 cut(s) 1507
NlaIII CATG 9 cut(s) 40, 716, 1156, 1372, 1463, 1598, 2251, 2368, 2459
NlaIV GGNNCC 3 cut(s) 402, 1582, 1618
NmuCI GTSAC 2 cut(s) 761, 1697
NspI RCATGY 3 cut(s) 716, 1463, 2459
NspV TTCGAA 1 cut(s) 82
OliI CACNNNNGTG 2 cut(s) 1138, 2233
PagI TCATGA 2 cut(s) 1368, 2364
PciI ACATGT 1 cut(s) 1459
PciSI GCTCTTC 1 cut(s) 1568
PctI GAATGC 1 cut(s) 524
PdmI GAANNNNTTC 2 cut(s) 914, 1850
PfeI GAWTC 9 cut(s) 22, 142, 449, 792, 958, 1084, 1728, 1894, 2179
PfoI TCCNGGA 1 cut(s) 1660
PkrI GCNGC 5 cut(s) 307, 569, 673, 1200, 1651
PleI GAGTC 3 cut(s) 233, 513, 2330
PpsI GAGTC 3 cut(s) 233, 513, 2330
PpuMI RGGWCCY 1 cut(s) 455
PscI ACATGT 1 cut(s) 1459
PshAI GACNNNNGTC 2 cut(s) 332, 504
PshBI ATTAAT 1 cut(s) 441
Psp124BI GAGCTC 1 cut(s) 1710
Psp5II RGGWCCY 1 cut(s) 455
Psp6I CCWGG 3 cut(s) 43, 969, 1905
PspGI CCWGG 3 cut(s) 43, 969, 1905
PspN4I GGNNCC 3 cut(s) 402, 1582, 1618
PspPI GGNCC 2 cut(s) 455, 1581
PspPPI RGGWCCY 1 cut(s) 455
PstI CTGCAG 2 cut(s) 676, 1654
PstNI CAGNNNCTG 1 cut(s) 483
PsuI RGATCY 2 cut(s) 1047, 2142
RsaI GTAC 6 cut(s) 473, 626, 1458, 2074, 2454, 2534
RsaNI GTAC 6 cut(s) 472, 625, 1457, 2073, 2453, 2533
RseI CAYNNNNRTG 2 cut(s) 1138, 2233
SacI GAGCTC 1 cut(s) 1710
SalI GTCGAC 1 cut(s) 2324
SapI GCTCTTC 1 cut(s) 1568
SaqAI TTAA 3 cut(s) 441, 1265, 1563
SatI GCNGC 5 cut(s) 306, 568, 672, 1199, 1650
Sau96I GGNCC 2 cut(s) 455, 1581
ScaI AGTACT 1 cut(s) 2534
SchI GAGTC 3 cut(s) 233, 514, 2331
ScrFI CCNGG 4 cut(s) 45, 971, 1662, 1907
SduI GDGCHC 2 cut(s) 1619, 1710
SexAI ACCWGGT 2 cut(s) 969, 1905
SfaNI GCATC 5 cut(s) 1321, 1425, 1522, 1636, 2421
SfcI CTRYAG 3 cut(s) 221, 672, 1650
SfuI TTCGAA 1 cut(s) 82
SinI GGWCC 1 cut(s) 455
SmiMI CAYNNNNRTG 2 cut(s) 1138, 2233
SmlI CTYRAG 1 cut(s) 1957
SmoI CTYRAG 1 cut(s) 1957
SpeI ACTAGT 1 cut(s) 1942
SsiI CCGC 4 cut(s) 189, 293, 1499, 1578
SspI AATATT 3 cut(s) 54, 362, 427
SspMI CTAG 8 cut(s) 122, 459, 641, 1202, 1355, 1943, 2313, 2536
SstI GAGCTC 1 cut(s) 1710
StyD4I CCNGG 4 cut(s) 43, 969, 1660, 1905
StyI CCWWGG 2 cut(s) 1335, 2331
TaiI ACGT 3 cut(s) 17, 304, 1693
TaqI TCGA 7 cut(s) 82, 774, 1408, 1710, 1879, 2325, 2404
TatI WGTACW 3 cut(s) 1456, 2452, 2532
TfiI GAWTC 9 cut(s) 22, 142, 449, 792, 958, 1084, 1728, 1894, 2179
Tru1I TTAA 3 cut(s) 441, 1265, 1563
Tru9I TTAA 3 cut(s) 441, 1265, 1563
TscAI CASTG 4 cut(s) 673, 879, 1815, 2491
TseFI GTSAC 2 cut(s) 761, 1697
TseI GCWGC 5 cut(s) 305, 567, 671, 1198, 1649
Tsp45I GTSAC 2 cut(s) 761, 1697
TspDTI ATGAA 8 cut(s) 228, 813, 1359, 1364, 1534, 1583, 1965, 2360
TspGWI ACGGA 2 cut(s) 1283, 1874
TspRI CASTG 4 cut(s) 673, 879, 1815, 2491
VpaK11BI GGWCC 1 cut(s) 455
VspI ATTAAT 1 cut(s) 441
XapI RAATTY 5 cut(s) 85, 745, 914, 1850, 1952
XbaI TCTAGA 1 cut(s) 2312
XceI RCATGY 3 cut(s) 716, 1463, 2459
XmiI GTMKAC 2 cut(s) 220, 2325
XmnI GAANNNNTTC 2 cut(s) 914, 1850
XspI CTAG 8 cut(s) 122, 459, 641, 1202, 1355, 1943, 2313, 2536
ZrmI AGTACT 1 cut(s) 2534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.