Rroxscaffold_1G00003360

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000130
Physical Location & Seq
Reverse (-)
4081898 .. 4086464
4567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00003360.1

Sequence Viewer

Length: 2388 bp
ATGGCTTTTACTGTACGGCTGCTTCTGCTTATTTTCTCATTGTTTCTGGTTCCAGTTTCTCTGTTTGCCCAGACTAATTATGGCAAAGTACCTGTGGGTGCTTCTCTCTCTGCAACTGCAGGTAACAGCTCCTCATGGCTTTCTCCTTCTGGTGATTTCGCCTTTGGTTTTCGACAACTTGAAAACAGTGATCTTTTCTTGCTTTCTGTATGGTATGCCAAGATTCCAGACAGAACCATAGTTTGGTATGCATTTGGGGATAGTCCTGCAGTTGTAGCTAATGGTTCACTTGTGAATTTGACTGCTGACAATGGACTAGTGCTTACAAGTCCTCAGGGTGTTCAGCTTTGGCAATCTGAGTCCATTTCTGGAGTTGTTGCCTTTGGGGTCTTGAATGATACAGGCAACTTTGTTCTTGAAGATGACAATTCAGAGAGCTTATGGGAGACCTTTAATAATCCAACTCATACCATTTTACCTGGACAGGATATTGAAAAAGGAGGGAAGCTTTTGTCTCAACAATCGGAGACTAACTTTTCGAAAGGAAGATTCGAGCTACGTATGCAAGATGATGGCAATCTTGTGCTTGTCACCATAAACTTGCCAACTGAGATTGCCAACAATCCTTACTATGCGAGTGACACTACTGGTGACTCTAATTCTTCTGCTGGTAGAAAGCTGGAGTTCAGTAGCTCAGGCTACATGTATATTCTGAGAGAGAATGGCGAAAAATTCACCATCAAGGAAGCAGAGACAGTCTCAACTAGGAGCTTCTATCTTAGAGCAACCCTCAACTTTGATGGGGTTTTTGCTAAATACGCTCACCCGAAAAATCCCACGGGTAATGTAAGCTGGAGTATTGTTTCGTCAGTGCCAGATAACATATGCAGTGCTACGACGGTTGAACATTCAGGTTTAGCTGTATGTGGCCTATACAGTATCTGCACTCTCCAAGATCAGAGGCCAACCTGTGAATGCCCACCAGGATACTCTTTATTGGATTCGAATGATCCGAATGGCAACTGCAAACCGGGTTTTGTACCAAGTTGTGAAGATGAGCTTAACTCCACAACTGATATATATGAAGTTCAGATGCTGAACAATACGGATTGGCCAAACTCTGATTATTTGCAGTTTCCTTCTACTCCAGAGAGTTGCAATCAATCTTGCTTTGAAGATTGTTTGTGTGCTGTTGCTATTTACAGAATTGAAACCTGTTGGAAAAAGAAGTTACCTCTTTCAAATGGGAGAGTGGATAGCAGGCTTAATTCACAGGCCTTCATCAAAGTCAGTAAGGGTAATTCAACTCAACAAGTTCCTCCAAAGCCAAGTCCAGATGATAAGAAGAAGAACAAGACCACTTCAATACTTTTGGGTACCTCTGTCTTTGTCAATTTTATTTTAATTGTTGCAGTTTGTCTCGGAGTTCTCATCTACCGGAAGAAACATGCAAGGTTCACTGGAAATGAGTTGGAAACAAATTTGGTTTGTTTTAGTTACAAGGAGCTTGAAGAAGCTACAAATGGATTCAGTGAAGAATTAGGGAGGGGTTCTTTTGGAGTTGTTTACAAAGGGATAATACATATTGGTTCTGGTCTCCAAGTAGCAGTGAAGAAGCTAAACTGTGTTATACAAGAGGGTGAGCAGGAATTCAAGACAGAGCTCAACGTAATCGGTAAGACACATCACAAGAATCTTGTTCAACTGGTTGGATATTGTGACGAGGGAGAGCAACTACCACGGTTACTTGTATATGAACTACTGAGCAATGGCACATTAGCAAGCTTCCTTTTTGGTGATACGAAACCCAGTTGGACGCAACGAATTGAGATTGCTTATGGAGTTGCCAATGGACTAATGTACTTGCATGAGGAGTGCAGAACACAGATTATACATTGTGATATAAAGCCTCAGAACATACTTCTCGATGAACATTACAATGCTCGGATCTCTGATTTCGGATTGGCAAAGCTTTTAATACTAAATCAGAGCCATACGCTTACTGCCATAAGAGGAACAAAAGGGTATGTTGCACCTGAGTGGTTTCGGAATATGCCAATCACTACCAAAGTCGATGTGTATAGCTTCGGCGTTGTGCTGCTAGAGATCATCTTCTGTAGGAGAAATGTTGATATGGAAAACAGATGTGAACACAAAGCAATTTTAGCCAATTGGGTTTATGATTGCTATCGAAAGGGTGTATTAGGTGAGGCTCTAGATCAAGAAGCTGAGGCCTTGTATGATACACGGAAGGCCTTGGAAAATATTGTGATGATTGCTATGTGGTGCATTCAAGAAGACCCGTCTCTTCGACCCACAATGAGGATGGTTGTGCAGATGCTTGAAGGAGCAGTGGAAGTACATGTTCCACCATGTCCATCCCCATAA

Protein Analysis

795

Amino Acids

88.24

Weight (kDa)

5.21

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 79 - 165 2.6e-12 D-mannose binding lectin
PK_Tyr_Ser-Thr PF07714 510 - 781 1.6e-44 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 511 - 779 6.9e-47 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000130)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g43780
malus_domestica MD03G1019500.v1.1 MD03G1019800.v1.1 MD03G1019900.v1.1 MD03G1020000.v1.1 MD03G1020200.v1.1 MD03G1020300.v1.1 MD05G1226500.v1.1 MD05G1226600.v1.1 MD05G1226700.v1.1 MD05G1226800.v1.1 MD09G1060200.v1.1 MD11G1021100.v1.1 MD11G1021300.v1.1 MD11G1021400.v1.1 MD11G1021500.v1.1 MD11G1024600.v1.1 MD11G1026700.v1.1
prunus_persica Prupe.6G016300_v2.0.a1 Prupe.6G016600_v2.0.a1 Prupe.6G016700_v2.0.a1 Prupe.6G017000_v2.0.a1 Prupe.6G017100_v2.0.a1 Prupe.6G017200_v2.0.a1 Prupe.6G017300_v2.0.a1 Prupe.6G017400_v2.0.a1 Prupe.6G017500_v2.0.a1
pyrus_communis pycom02g26630 pycom03g01670 pycom03g01710 pycom03g01720 pycom05g20850 pycom11g01710 pycom11g01730 pycom11g01740 pycom11g01960
rosa_chinensis RchiOBHm_Chr1g0313471 RchiOBHm_Chr1g0313491 RchiOBHm_Chr1g0313521 RchiOBHm_Chr1g0329041 RchiOBHm_Chr5g0077581 RchiOBHm_Chr5g0077681 RchiOBHm_Chr5g0077751 RchiOBHm_Chr5g0077771 RchiOBHm_Chr5g0078381 RchiOBHm_Chr5g0078421 RchiOBHm_Chr5g0078481 RchiOBHm_Chr5g0078491 RchiOBHm_Chr5g0078511 RchiOBHm_Chr5g0078561 RchiOBHm_Chr5g0078581 RchiOBHm_Chr5g0078621 RchiOBHm_Chr5g0078631 RchiOBHm_Chr5g0078641 RchiOBHm_Chr5g0078681 RchiOBHm_Chr5g0078741 RchiOBHm_Chr5g0078781 RchiOBHm_Chr5g0078791 RchiOBHm_Chr5g0078821 RchiOBHm_Chr5g0078851 RchiOBHm_Chr5g0078861 RchiOBHm_Chr5g0078881 RchiOBHm_Chr5g0078911 RchiOBHm_Chr5g0078921 RchiOBHm_Chr5g0078931 RchiOBHm_Chr5g0078951 RchiOBHm_Chr5g0079061 RchiOBHm_Chr5g0079071 RchiOBHm_Chr5g0079101 RchiOBHm_Chr5g0079111 RchiOBHm_Chr5g0079131 RchiOBHm_Chr5g0079151 RchiOBHm_Chr5g0079161 RchiOBHm_Chr5g0079211 RchiOBHm_Chr5g0079241 RchiOBHm_Chr7g0223671
rosa_laevigata RLG00000001266 RLG00000036708 RLG00000036719 RLG00000036772 RLG00000036773 RLG00000036776 RLG00000036780 RLG00000036781 RLG00000036783 RLG00000036788 RLG00000036790 RLG00000036792 RLG00000036794 RLG00000036797 RLG00000036798 RLG00000036800 RLG00000036801 RLG00000036802 RLG00000036803 RLG00000036805 RLG00000036807 RLG00000036808
rosa_multiflora Rmu_co7998958.1_g000001 Rmu_co8175864.1_g000001 Rmu_co8214884.1_g000001 Rmu_co8225446.1_g000001 Rmu_sc0000607.1_g000011 Rmu_sc0000607.1_g000012 Rmu_sc0000631.1_g000001 Rmu_sc0000631.1_g000003 Rmu_sc0000631.1_g000010 Rmu_sc0000631.1_g000012 Rmu_sc0000631.1_g000020 Rmu_sc0001347.1_g000014 Rmu_sc0002187.1_g000011 Rmu_sc0002187.1_g000014 Rmu_sc0002187.1_g000024 Rmu_sc0002187.1_g000025 Rmu_sc0003413.1_g000051 Rmu_sc0004140.1_g000002 Rmu_sc0004140.1_g000003 Rmu_sc0004140.1_g000015 Rmu_sc0004140.1_g000026 Rmu_sc0004140.1_g000036 Rmu_sc0004140.1_g000042 Rmu_sc0004140.1_g000046 Rmu_sc0004140.1_g000048 Rmu_sc0004277.1_g000002 Rmu_sc0008700.1_g000002 Rmu_sc0011280.1_g000001 Rmu_sc0011280.1_g000002 Rmu_sc0011280.1_g000009 Rmu_sc0012487.1_g000003 Rmu_sc0012487.1_g000007 Rmu_sc0012487.1_g000009 Rmu_sc0012931.1_g000001 Rmu_sc0012931.1_g000002 Rmu_sc0012931.1_g000009 Rmu_sc0015122.1_g000002 Rmu_sc0022488.1_g000001 Rmu_ssc0000425.1_g000018
rosa_roxburghii Rroxscaffold_164G00436380 Rroxscaffold_1G00003360 Rroxscaffold_1G00003370 Rroxscaffold_1G00003660 Rroxscaffold_1G00003790 Rroxscaffold_1G00003840 Rroxscaffold_1G00003950 Rroxscaffold_1G00004380 Rroxscaffold_4G00332170
rosa_rugosa Rorug05G0455800 Rorug05G0456500 Rorug05G0460800 Rorug05G0461000 Rorug05G0461200 Rorug05G0461200 Rorug05G0461300 Rorug05G0461600 Rorug05G0461700 Rorug05G0461900 Rorug05G0462000 Rorug05G0462400 Rorug05G0462500 Rorug05G0462800 Rorug05G0463200.1 Rorug05G0463300
rosa_samantha Rh1CG000600 Rh1CG008300 Rh1CG360600 Rh5AG509700 Rh5AG509800 Rh5AG510700 Rh5AG515000 Rh5AG515600 Rh5BG531300 Rh5BG531400 Rh5BG533600 Rh5BG538200 Rh5BG538300 Rh5BG538400 Rh5BG538500 Rh5BG539100 Rh5BG539200 Rh5BG539400 Rh5DG545100 Rh5DG549000 Rh5DG549200 Rh5DG549500 Rh5DG549600 Rh5DG549800 Rh5DG549900 Rh5DG550000 Rh5DG550200 Rh5DG550300 Rh7AG436900 Rh7CG456800
rosa_wichuraiana Rw0G003110 Rw0G003120 Rw0G005240 Rw0G023800 Rw0G023830 Rw1G007840 Rw5G000030 Rw5G047760 Rw5G047780 Rw5G047810 Rw5G047900 Rw5G047920 Rw5G047970 Rw5G047980 Rw5G048000 Rw5G048010 Rw5G048030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 110
Acc65I GGTACC 1 cut(s) 1376
AccB1I GGYRCC 1 cut(s) 1376
AccB7I CCANNNNNTGG 1 cut(s) 243
AclWI GGATC 2 cut(s) 1004, 1955
AcoI YGGCCR 1 cut(s) 1112
AcsI RAATTY 4 cut(s) 295, 731, 1480, 1649
AfaI GTAC 6 cut(s) 15, 90, 1041, 1378, 1862, 2361
AfiI CCNNNNNNNGG 2 cut(s) 243, 2322
AflIII ACRYGT 2 cut(s) 702, 2362
AhlI ACTAGT 1 cut(s) 316
AjnI CCWGG 2 cut(s) 478, 982
AjuI GAANNNNNNNTTGG 2 cut(s) 226, 258
AleI CACNNNNGTG 1 cut(s) 2038
Alw21I GWGCWC 1 cut(s) 1665
Alw26I GTCTC 8 cut(s) 440, 519, 521, 746, 763, 1424, 1601, 2310
AlwI GGATC 2 cut(s) 1004, 1955
AlwNI CAGNNNCTG 2 cut(s) 942, 1096
AoxI GGCC 6 cut(s) 928, 962, 1112, 1275, 2232, 2253
ApeKI GCWGC 2 cut(s) 19, 2098
ApoI RAATTY 4 cut(s) 295, 731, 1480, 1649
ArsI GACNNNNNNTTYG 2 cut(s) 520, 552
Asp718I GGTACC 1 cut(s) 1376
AsuC2I CCSGG 1 cut(s) 1032
AsuHPI GGTGA 8 cut(s) 164, 583, 662, 727, 815, 1652, 1808, 2219
AsuII TTCGAA 2 cut(s) 539, 1004
AxyI CCTNAGG 1 cut(s) 333
BaeI ACNNNNGTAYC 2 cut(s) 1368, 1401
BalI TGGCCA 1 cut(s) 1114
BanI GGYRCC 1 cut(s) 1376
BanII GRGCYC 1 cut(s) 1665
BarI GAAGNNNNNNTAC 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 2304
Bbv12I GWGCWC 1 cut(s) 1665
BbvCI CCTCAGC 1 cut(s) 2229
BbvI GCAGC 2 cut(s) 6, 2085
BccI CCATC 5 cut(s) 566, 746, 794, 2320, 2386
BceAI ACGGC 1 cut(s) 32
BciT130I CCWGG 2 cut(s) 480, 984
BciVI GTATCC 1 cut(s) 980
BcnI CCSGG 1 cut(s) 1032
BcoDI GTCTC 8 cut(s) 440, 519, 521, 746, 763, 1424, 1601, 2310
BcuI ACTAGT 1 cut(s) 316
BfaI CTAG 4 cut(s) 317, 765, 2102, 2216
BfmI CTRYAG 3 cut(s) 117, 267, 2116
BfuAI ACCTGC 1 cut(s) 110
BfuI GTATCC 1 cut(s) 980
BisI GCNGC 2 cut(s) 20, 2099
BlsI GCNGC 2 cut(s) 21, 2100
Bme1390I CCNGG 3 cut(s) 480, 984, 1032
BmiI GGNNCC 2 cut(s) 51, 1378
BmrFI CCNGG 3 cut(s) 480, 984, 1032
BmrI ACTGGG 1 cut(s) 1803
BmsI GCATC 2 cut(s) 1083, 2328
BmuI ACTGGG 1 cut(s) 1803
BpiI GAAGAC 1 cut(s) 2304
BplI GAGNNNNNCTC 6 cut(s) 743, 775, 774, 806, 1049, 1081
BpmI CTGGAG 4 cut(s) 390, 701, 874, 1131
Bpu10I CCTNAGC 2 cut(s) 694, 2229
Bpu14I TTCGAA 2 cut(s) 539, 1004
BpuMI CCSGG 1 cut(s) 1032
BsaAI YACGTR 1 cut(s) 560
BsaBI GATNNNNATC 2 cut(s) 576, 2187
BsaI GGTCTC 2 cut(s) 440, 1601
BsaJI CCNNGG 3 cut(s) 837, 1739, 2256
BsaWI WCCGGW 1 cut(s) 1437
Bsc4I CCNNNNNNNGG 2 cut(s) 243, 2322
Bse1I ACTGG 5 cut(s) 53, 652, 1465, 1710, 1809
Bse21I CCTNAGG 1 cut(s) 333
Bse3DI GCAATG 1 cut(s) 1774
Bse8I GATNNNNATC 2 cut(s) 576, 2187
BseBI CCWGG 2 cut(s) 480, 984
BseDI CCNNGG 3 cut(s) 837, 1739, 2256
BseGI GGATG 2 cut(s) 2331, 2378
BseJI GATNNNNATC 2 cut(s) 576, 2187
BseLI CCNNNNNNNGG 2 cut(s) 243, 2322
BseMI GCAATG 1 cut(s) 1774
BseMII CTCAG 9 cut(s) 347, 348, 600, 704, 708, 1754, 1925, 2028, 2220
BseNI ACTGG 5 cut(s) 53, 652, 1465, 1710, 1809
BseRI GAGGAG 2 cut(s) 121, 1886
BseXI GCAGC 2 cut(s) 6, 2085
BsgI GTGCAG 3 cut(s) 928, 1897, 2354
BshFI GGCC 6 cut(s) 930, 964, 1114, 1277, 2234, 2255
BshNI GGYRCC 1 cut(s) 1376
BsiHKAI GWGCWC 1 cut(s) 1665
BsiSI CCGG 2 cut(s) 1031, 1438
BslI CCNNNNNNNGG 2 cut(s) 243, 2322
BsmAI GTCTC 8 cut(s) 440, 519, 521, 746, 763, 1424, 1601, 2310
BsmBI CGTCTC 1 cut(s) 2310
BsmI GAATGC 2 cut(s) 980, 2289
BsnI GGCC 6 cut(s) 930, 964, 1114, 1277, 2234, 2255
Bso31I GGTCTC 2 cut(s) 440, 1601
Bsp119I TTCGAA 2 cut(s) 539, 1004
Bsp1286I GDGCHC 1 cut(s) 1665
Bsp143I GATC 6 cut(s) 190, 955, 1009, 1947, 2106, 2218
BspANI GGCC 6 cut(s) 930, 964, 1114, 1277, 2234, 2255
BspCNI CTCAG 9 cut(s) 346, 349, 601, 705, 707, 1755, 1924, 2029, 2221
BspLI GGNNCC 2 cut(s) 51, 1378
BspMAI CTGCAG 2 cut(s) 121, 271
BspMI ACCTGC 1 cut(s) 110
BspPI GGATC 2 cut(s) 1004, 1955
BspT104I TTCGAA 2 cut(s) 539, 1004
BspT107I GGYRCC 1 cut(s) 1376
BspTNI GGTCTC 2 cut(s) 440, 1601
BsrDI GCAATG 1 cut(s) 1774
BsrI ACTGG 5 cut(s) 53, 652, 1465, 1710, 1809
BssECI CCNNGG 3 cut(s) 837, 1739, 2256
BssMI GATC 6 cut(s) 190, 955, 1009, 1947, 2106, 2218
BssT1I CCWWGG 1 cut(s) 2256
Bst2UI CCWGG 2 cut(s) 480, 984
Bst4CI ACNGT 7 cut(s) 13, 188, 757, 901, 938, 1625, 1743
Bst6I CTCTTC 1 cut(s) 2313
BstBAI YACGTR 1 cut(s) 560
BstBI TTCGAA 2 cut(s) 539, 1004
BstC8I GCNNGC 2 cut(s) 1262, 1783
BstDSI CCRYGG 2 cut(s) 837, 1739
BstF5I GGATG 2 cut(s) 2331, 2378
BstKTI GATC 6 cut(s) 193, 958, 1012, 1950, 2109, 2221
BstMAI GTCTC 8 cut(s) 440, 519, 521, 746, 763, 1424, 1601, 2310
BstMBI GATC 6 cut(s) 190, 955, 1009, 1947, 2106, 2218
BstMWI GCNNNNNNNGC 5 cut(s) 25, 275, 562, 818, 2165
BstNI CCWGG 2 cut(s) 480, 984
BstNSI RCATGY 3 cut(s) 706, 1451, 2366
BstSCI CCNGG 3 cut(s) 478, 982, 1030
BstSFI CTRYAG 3 cut(s) 117, 267, 2116
BstSNI TACGTA 1 cut(s) 560
BstV1I GCAGC 2 cut(s) 6, 2085
BstV2I GAAGAC 1 cut(s) 2304
BstX2I RGATCY 1 cut(s) 1947
BstYI RGATCY 1 cut(s) 1947
Bsu36I CCTNAGG 1 cut(s) 333
BsuI GTATCC 1 cut(s) 980
BsuRI GGCC 6 cut(s) 930, 964, 1114, 1277, 2234, 2255
BtgI CCRYGG 2 cut(s) 837, 1739
BtsCI GGATG 2 cut(s) 2331, 2378
BtsI GCAGTG 3 cut(s) 895, 1614, 2358
BtsIMutI CAGTG 7 cut(s) 193, 876, 895, 1458, 1537, 1614, 2358
BveI ACCTGC 1 cut(s) 110
Cac8I GCNNGC 2 cut(s) 1262, 1783
CaiI CAGNNNCTG 2 cut(s) 942, 1096
CseI GACGC 1 cut(s) 1825
Csp6I GTAC 6 cut(s) 14, 89, 1040, 1377, 1861, 2360
CviAII CATG 6 cut(s) 135, 703, 1448, 1868, 2363, 2373
CviQI GTAC 6 cut(s) 14, 89, 1040, 1377, 1861, 2360
DpnI GATC 6 cut(s) 192, 957, 1011, 1949, 2108, 2220
DpnII GATC 6 cut(s) 190, 955, 1009, 1947, 2106, 2218
EaeI YGGCCR 1 cut(s) 1112
Eam1104I CTCTTC 1 cut(s) 2313
EarI CTCTTC 1 cut(s) 2313
Ecl136II GAGCTC 1 cut(s) 1663
Eco105I TACGTA 1 cut(s) 560
Eco130I CCWWGG 1 cut(s) 2256
Eco147I AGGCCT 3 cut(s) 1277, 2234, 2255
Eco24I GRGCYC 1 cut(s) 1665
Eco31I GGTCTC 2 cut(s) 440, 1601
Eco53kI GAGCTC 1 cut(s) 1663
Eco81I CCTNAGG 1 cut(s) 333
EcoICRI GAGCTC 1 cut(s) 1663
EcoRI GAATTC 1 cut(s) 1649
EcoRII CCWGG 2 cut(s) 478, 982
EcoT14I CCWWGG 1 cut(s) 2256
EcoT22I ATGCAT 1 cut(s) 253
EcoT38I GRGCYC 1 cut(s) 1665
ErhI CCWWGG 1 cut(s) 2256
Esp3I CGTCTC 1 cut(s) 2310
FaeI CATG 6 cut(s) 138, 706, 1451, 1871, 2366, 2376
FalI AAGNNNNNCTT 2 cut(s) 1044, 1076
FatI CATG 6 cut(s) 134, 702, 1447, 1867, 2362, 2372
FauNDI CATATG 1 cut(s) 884
Fnu4HI GCNGC 2 cut(s) 20, 2099
FokI GGATG 2 cut(s) 2338, 2365
FriOI GRGCYC 1 cut(s) 1665
Fsp4HI GCNGC 2 cut(s) 20, 2099
FspBI CTAG 4 cut(s) 317, 765, 2102, 2216
GluI GCNGC 2 cut(s) 20, 2099
GsuI CTGGAG 4 cut(s) 390, 701, 874, 1131
HaeIII GGCC 6 cut(s) 930, 964, 1114, 1277, 2234, 2255
HapII CCGG 2 cut(s) 1031, 1438
HgaI GACGC 1 cut(s) 1825
Hin1II CATG 6 cut(s) 138, 706, 1451, 1871, 2366, 2376
HindIII AAGCTT 3 cut(s) 506, 1783, 1970
HinfI GANTC 7 cut(s) 223, 359, 549, 653, 1001, 1527, 1693
HpaII CCGG 2 cut(s) 1031, 1438
HphI GGTGA 8 cut(s) 164, 583, 662, 727, 815, 1652, 1808, 2219
Hpy166II GTNNAC 4 cut(s) 287, 1458, 1567, 2150
Hpy8I GTNNAC 4 cut(s) 287, 1458, 1567, 2150
Hpy99I CGWCG 1 cut(s) 901
HpyAV CCTTC 5 cut(s) 156, 1149, 1288, 2245, 2339
HpyCH4III ACNGT 7 cut(s) 13, 188, 757, 901, 938, 1625, 1743
HpyCH4IV ACGT 2 cut(s) 559, 1668
HpyF10VI GCNNNNNNNGC 5 cut(s) 25, 275, 562, 818, 2165
HpySE526I ACGT 2 cut(s) 559, 1668
Hsp92II CATG 6 cut(s) 138, 706, 1451, 1871, 2366, 2376
KpnI GGTACC 1 cut(s) 1380
Kzo9I GATC 6 cut(s) 190, 955, 1009, 1947, 2106, 2218
LmnI GCTCC 4 cut(s) 134, 768, 1504, 2348
Lsp1109I GCAGC 2 cut(s) 6, 2085
LweI GCATC 2 cut(s) 1083, 2328
MaeI CTAG 4 cut(s) 317, 765, 2102, 2216
MaeII ACGT 2 cut(s) 559, 1668
MaeIII GTNAC 8 cut(s) 122, 589, 638, 650, 1230, 1496, 1718, 1743
MalI GATC 6 cut(s) 192, 957, 1011, 1949, 2108, 2220
MboI GATC 6 cut(s) 190, 955, 1009, 1947, 2106, 2218
MfeI CAATTG 1 cut(s) 2170
MflI RGATCY 1 cut(s) 1947
MhlI GDGCHC 1 cut(s) 1665
MlsI TGGCCA 1 cut(s) 1114
MluNI TGGCCA 1 cut(s) 1114
MlyI GAGTC 2 cut(s) 368, 647
MmeI TCCRAC 5 cut(s) 485, 1199, 1452, 1690, 1793
Mox20I TGGCCA 1 cut(s) 1114
Mph1103I ATGCAT 1 cut(s) 253
MscI TGGCCA 1 cut(s) 1114
MseI TTAA 5 cut(s) 453, 1062, 1266, 1403, 1976
MslI CAYNNNNRTG 2 cut(s) 1938, 2038
Msp20I TGGCCA 1 cut(s) 1114
MspI CCGG 2 cut(s) 1031, 1438
MspR9I CCNGG 3 cut(s) 480, 984, 1032
MunI CAATTG 1 cut(s) 2170
Mva1269I GAATGC 2 cut(s) 980, 2289
MvaI CCWGG 2 cut(s) 480, 984
MwoI GCNNNNNNNGC 5 cut(s) 25, 275, 562, 818, 2165
NciI CCSGG 1 cut(s) 1032
NdeI CATATG 1 cut(s) 884
NdeII GATC 6 cut(s) 190, 955, 1009, 1947, 2106, 2218
NlaIII CATG 6 cut(s) 138, 706, 1451, 1871, 2366, 2376
NlaIV GGNNCC 2 cut(s) 51, 1378
NmuCI GTSAC 4 cut(s) 589, 638, 650, 1718
NsiI ATGCAT 1 cut(s) 253
NspI RCATGY 3 cut(s) 706, 1451, 2366
NspV TTCGAA 2 cut(s) 539, 1004
OliI CACNNNNGTG 1 cut(s) 2038
PceI AGGCCT 3 cut(s) 1277, 2234, 2255
PciI ACATGT 2 cut(s) 702, 2362
PcsI WCGNNNNNNNCGW 1 cut(s) 1010
PctI GAATGC 2 cut(s) 980, 2289
PfeI GAWTC 5 cut(s) 223, 549, 1001, 1527, 1693
PflMI CCANNNNNTGG 1 cut(s) 243
PkrI GCNGC 2 cut(s) 21, 2100
PleI GAGTC 2 cut(s) 367, 647
PpsI GAGTC 2 cut(s) 367, 647
Ppu21I YACGTR 1 cut(s) 560
PscI ACATGT 2 cut(s) 702, 2362
Psp124BI GAGCTC 1 cut(s) 1665
Psp6I CCWGG 2 cut(s) 478, 982
PspGI CCWGG 2 cut(s) 478, 982
PspN4I GGNNCC 2 cut(s) 51, 1378
PstI CTGCAG 2 cut(s) 121, 271
PstNI CAGNNNCTG 2 cut(s) 942, 1096
PsuI RGATCY 1 cut(s) 1947
RsaI GTAC 6 cut(s) 15, 90, 1041, 1378, 1862, 2361
RsaNI GTAC 6 cut(s) 14, 89, 1040, 1377, 1861, 2360
RseI CAYNNNNRTG 2 cut(s) 1938, 2038
SacI GAGCTC 1 cut(s) 1665
SaqAI TTAA 5 cut(s) 453, 1062, 1266, 1403, 1976
SatI GCNGC 2 cut(s) 20, 2099
Sau3AI GATC 6 cut(s) 190, 955, 1009, 1947, 2106, 2218
SchI GAGTC 2 cut(s) 368, 647
ScrFI CCNGG 3 cut(s) 480, 984, 1032
SduI GDGCHC 1 cut(s) 1665
SfaNI GCATC 2 cut(s) 1083, 2328
SfcI CTRYAG 3 cut(s) 117, 267, 2116
SfuI TTCGAA 2 cut(s) 539, 1004
SmiMI CAYNNNNRTG 2 cut(s) 1938, 2038
SnaBI TACGTA 1 cut(s) 560
SpeI ACTAGT 1 cut(s) 316
SseBI AGGCCT 3 cut(s) 1277, 2234, 2255
SspI AATATT 1 cut(s) 2266
SspMI CTAG 4 cut(s) 317, 765, 2102, 2216
SstI GAGCTC 1 cut(s) 1665
StuI AGGCCT 3 cut(s) 1277, 2234, 2255
StyD4I CCNGG 3 cut(s) 478, 982, 1030
StyI CCWWGG 1 cut(s) 2256
TaaI ACNGT 7 cut(s) 13, 188, 757, 901, 938, 1625, 1743
TaiI ACGT 2 cut(s) 562, 1671
TaqI TCGA 8 cut(s) 172, 539, 552, 1004, 1926, 2073, 2191, 2311
TatI WGTACW 2 cut(s) 1860, 2359
TfiI GAWTC 5 cut(s) 223, 549, 1001, 1527, 1693
Tru1I TTAA 5 cut(s) 453, 1062, 1266, 1403, 1976
Tru9I TTAA 5 cut(s) 453, 1062, 1266, 1403, 1976
TscAI CASTG 7 cut(s) 193, 876, 895, 1465, 1537, 1614, 2358
TseFI GTSAC 4 cut(s) 589, 638, 650, 1718
TseI GCWGC 2 cut(s) 19, 2098
Tsp45I GTSAC 4 cut(s) 589, 638, 650, 1718
TspDTI ATGAA 4 cut(s) 1098, 1270, 1770, 1944
TspGWI ACGGA 2 cut(s) 1121, 2263
TspRI CASTG 7 cut(s) 193, 876, 895, 1465, 1537, 1614, 2358
Van91I CCANNNNNTGG 1 cut(s) 243
XapI RAATTY 4 cut(s) 295, 731, 1480, 1649
XbaI TCTAGA 1 cut(s) 2215
XceI RCATGY 3 cut(s) 706, 1451, 2366
XcmI CCANNNNNNNNNTGG 2 cut(s) 77, 2323
XspI CTAG 4 cut(s) 317, 765, 2102, 2216
Zsp2I ATGCAT 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.