MD03G1079200.v1.1

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000148
Physical Location & Seq
Reverse (-)
6394219 .. 6394803
585 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1079200.v1.1.491

Sequence Viewer

Length: 585 bp
ATGGTTACTAATCCTACTATTTGCGTGAAGGCCTTGGTGGACAAGGGAAGCAACAAGGTTATTTTCGTAGAGTCTGGTAATGATTTGGTTGATACTCTCTTCAGTTACCTAACAATCCCCATGGGAACAGTTATCGATCTTGCTCGTAAACATTCAGATCCTGCTGTCATTGGCTGCATGAAAAACTTGTACGCTAGTGTTGAGAGTATTGGGGAAGAGGAATTTTTGGGTAATATATTTAAGGACATGCTGTTGCATCCTCGCAGTGCAGCCGAACCTCTCTACAGGAACCTCAACTTCAAACTTCATAATGTTAGGCCCCTGCCCTACTATATGTGCTTTGATTCCGGATCATGCAAGTTCTTGAGTTATGAATCGGATGTCCAGTGCCCTCATCATTTGTTCCATAACGAACCAATCATGGACCGGGATAATTGCCCTTCAGTGGGGGCTTCTCAAGATGGAAGTGATTTTATAAAAGGGCGACTCTGGCTTACAATTACTGATGATTTACAAGTGTTTTACTCAGCTTGGAGTCGTCGACGTGAATGCTATGGAGGAGTTCACTTTCAATATCGGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

195

Amino Acids

22.08

Weight (kDa)

5.95

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 4 - 180 7.3e-45 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000148)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11145 AT3G09110 AT3G09120 AT3G09140 AT3G09140 AT5G01120 AT5G01130 AT5G01140 AT5G01150 AT5G37320 AT5G43240 AT5G43240 AT5G43240 AT5G43240
fragaria_vesca FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37580 FvH4_3g37580 FvH4_3g37590 FvH4_3g37590 FvH4_3g37590 FvH4_3g37601 FvH4_3g37610 FvH4_3g37610 FvH4_3g37640 FvH4_3g37640 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430
malus_domestica MD03G1079100.v1.1 MD03G1079200.v1.1 MD03G1079300.v1.1 MD03G1079500.v1.1 MD03G1079600.v1.1 MD03G1079700.v1.1 MD03G1079800.v1.1 MD11G1083300.v1.1 MD11G1083400.v1.1 MD11G1083500.v1.1 MD11G1083600.v1.1 MD11G1083700.v1.1 MD11G1083900.v1.1 MD11G1084000.v1.1 MD11G1084200.v1.1 MD11G1084300.v1.1
prunus_persica Prupe.1G077500_v2.0.a1 Prupe.6G063700_v2.0.a1 Prupe.6G063800_v2.0.a1 Prupe.6G063800_v2.0.a1 Prupe.6G063900_v2.0.a1 Prupe.6G064000_v2.0.a1 Prupe.6G064100_v2.0.a1 Prupe.6G064100_v2.0.a1 Prupe.6G064300_v2.0.a1 Prupe.6G064400_v2.0.a1
pyrus_communis pycom03g06310 pycom03g06320 pycom03g06330 pycom03g06340 pycom03g06350 pycom11g07140 pycom11g07160 pycom11g07170 pycom11g07190 pycom11g07200 pycom11g07230 pycom11g07240 pycom11g07250 pycom11g07260 pycom11g07270 pycom11g07280
rosa_chinensis RchiOBHm_Chr3g0490491 RchiOBHm_Chr5g0055481 RchiOBHm_Chr5g0067411 RchiOBHm_Chr5g0067441 RchiOBHm_Chr5g0067471 RchiOBHm_Chr5g0067491 RchiOBHm_Chr5g0067511 RchiOBHm_Chr5g0067521 RchiOBHm_Chr5g0067571 RchiOBHm_Chr5g0067581 RchiOBHm_Chr5g0067661 RchiOBHm_Chr5g0067671 RchiOBHm_Chr5g0067691 RchiOBHm_Chr5g0067721 RchiOBHm_Chr5g0067751 RchiOBHm_Chr5g0067801 RchiOBHm_Chr5g0067821 RchiOBHm_Chr5g0067831 RchiOBHm_Chr5g0067841 RchiOBHm_Chr5g0067861 RchiOBHm_Chr5g0067891 RchiOBHm_Chr5g0068351 RchiOBHm_Chr6g0278351
rosa_laevigata RLG00000013235 RLG00000022886 RLG00000035934 RLG00000035935 RLG00000035936 RLG00000035938 RLG00000035939 RLG00000035940 RLG00000035942 RLG00000035968
rosa_multiflora Rmu_sc0000230.1_g000001 Rmu_sc0000435.1_g000052 Rmu_sc0000435.1_g000053 Rmu_sc0000435.1_g000057 Rmu_sc0000435.1_g000067 Rmu_sc0000435.1_g000080 Rmu_sc0001016.1_g000006 Rmu_sc0001418.1_g000006 Rmu_sc0001418.1_g000008 Rmu_sc0002253.1_g000012 Rmu_sc0002253.1_g000013 Rmu_sc0002253.1_g000038 Rmu_sc0002253.1_g000039 Rmu_sc0002253.1_g000044 Rmu_sc0002489.1_g000055 Rmu_sc0003862.1_g000013 Rmu_sc0007131.1_g000003 Rmu_sc0007131.1_g000004 Rmu_sc0008054.1_g000001 Rmu_sc0009958.1_g000001 Rmu_sc0016063.1_g000001 Rmu_sc0022825.1_g000001
rosa_roxburghii Rroxscaffold_1G00012750 Rroxscaffold_1G00012810 Rroxscaffold_1G00012820 Rroxscaffold_1G00012860 Rroxscaffold_1G00012880 Rroxscaffold_1G00012920 Rroxscaffold_1G00012930 Rroxscaffold_1G00012940 Rroxscaffold_1G00013430 Rroxscaffold_1G00013440 Rroxscaffold_1G00013460 Rroxscaffold_1G00013510 Rroxscaffold_1G00013530 Rroxscaffold_1G00013550 Rroxscaffold_1G00013570 Rroxscaffold_1G00013580 Rroxscaffold_1G00013610 Rroxscaffold_1G00013620 Rroxscaffold_1G00013630 Rroxscaffold_1G00013680 Rroxscaffold_7G00190460
rosa_rugosa Rorug05G0192300 Rorug05G0192400 Rorug05G0192500 Rorug05G0192600 Rorug05G0192700 Rorug05G0384600 Rorug05G0385300 Rorug05G0385400 Rorug05G0385400 Rorug05G0385500 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385800 Rorug05G0386000 Rorug05G0389000 Rorug05G0389100 Rorug05G0397000 Rorug05G0397100
rosa_samantha Rh5BG461400 Rh5BG461600 Rh5BG461700 Rh5BG461900 Rh5BG462100 Rh6AG225700 Rh6AG225800
rosa_wichuraiana Rw0G017720 Rw3G025960 Rw5G041500 Rw5G041510 Rw5G041520 Rw5G041530 Rw5G041540 Rw5G041580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 476
AccI GTMKAC 1 cut(s) 541
AccIII TCCGGA 1 cut(s) 347
AclWI GGATC 2 cut(s) 152, 358
AcsI RAATTY 1 cut(s) 221
AcuI CTGAAG 2 cut(s) 85, 426
AfaI GTAC 1 cut(s) 191
AfiI CCNNNNNNNGG 2 cut(s) 445, 446
AgsI TTSAA 2 cut(s) 301, 572
AjiI CACGTC 1 cut(s) 545
AluBI AGCT 1 cut(s) 530
AluI AGCT 1 cut(s) 530
AlwI GGATC 2 cut(s) 152, 358
AlwNI CAGNNNCTG 1 cut(s) 161
Aor13HI TCCGGA 1 cut(s) 347
AoxI GGCC 2 cut(s) 30, 317
ApeKI GCWGC 2 cut(s) 174, 269
ApoI RAATTY 1 cut(s) 221
AspS9I GGNCC 2 cut(s) 318, 424
AsuC2I CCSGG 1 cut(s) 428
AvaII GGWCC 1 cut(s) 424
BaeGI GKGCMC 1 cut(s) 392
BbvI GCAGC 2 cut(s) 161, 281
BccI CCATC 1 cut(s) 455
BcgI CGANNNNNNTGC 2 cut(s) 531, 565
BcnI CCSGG 1 cut(s) 428
BfaI CTAG 1 cut(s) 195
BfmI CTRYAG 1 cut(s) 283
BisI GCNGC 2 cut(s) 175, 270
BlsI GCNGC 2 cut(s) 176, 271
Bme1390I CCNGG 1 cut(s) 428
Bme18I GGWCC 1 cut(s) 424
BmgBI CACGTC 1 cut(s) 545
BmgT120I GGNCC 2 cut(s) 318, 424
BmiI GGNNCC 2 cut(s) 290, 320
BmrFI CCNGG 1 cut(s) 428
BmsI GCATC 1 cut(s) 265
BpuEI CTTGAG 2 cut(s) 385, 441
BpuMI CCSGG 1 cut(s) 428
Bsa29I ATCGAT 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 33, 120
BsaWI WCCGGW 1 cut(s) 347
Bsc4I CCNNNNNNNGG 2 cut(s) 445, 446
Bse1I ACTGG 1 cut(s) 385
BseAI TCCGGA 1 cut(s) 347
BseCI ATCGAT 1 cut(s) 135
BseDI CCNNGG 2 cut(s) 33, 120
BseGI GGATG 2 cut(s) 256, 385
BseLI CCNNNNNNNGG 2 cut(s) 445, 446
BseMII CTCAG 1 cut(s) 540
BseNI ACTGG 1 cut(s) 385
BseRI GAGGAG 1 cut(s) 573
BseSI GKGCMC 1 cut(s) 392
BseXI GCAGC 2 cut(s) 161, 281
BsgI GTGCAG 1 cut(s) 288
BshFI GGCC 2 cut(s) 32, 319
BshVI ATCGAT 1 cut(s) 135
BsiSI CCGG 2 cut(s) 348, 427
BslI CCNNNNNNNGG 2 cut(s) 445, 446
BsmI GAATGC 1 cut(s) 554
BsnI GGCC 2 cut(s) 32, 319
Bsp1286I GDGCHC 1 cut(s) 392
Bsp13I TCCGGA 1 cut(s) 347
Bsp143I GATC 3 cut(s) 136, 157, 350
Bsp19I CCATGG 1 cut(s) 120
BspANI GGCC 2 cut(s) 32, 319
BspCNI CTCAG 1 cut(s) 539
BspDI ATCGAT 1 cut(s) 135
BspEI TCCGGA 1 cut(s) 347
BspLI GGNNCC 2 cut(s) 290, 320
BspPI GGATC 2 cut(s) 152, 358
BsrI ACTGG 1 cut(s) 385
BssECI CCNNGG 2 cut(s) 33, 120
BssMI GATC 3 cut(s) 136, 157, 350
BssT1I CCWWGG 2 cut(s) 33, 120
Bst4CI ACNGT 1 cut(s) 130
Bst6I CTCTTC 2 cut(s) 104, 210
BstDEI CTNAG 1 cut(s) 526
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 2 cut(s) 256, 385
BstKTI GATC 3 cut(s) 139, 160, 353
BstMBI GATC 3 cut(s) 136, 157, 350
BstMWI GCNNNNNNNGC 1 cut(s) 490
BstNSI RCATGY 1 cut(s) 250
BstSCI CCNGG 1 cut(s) 426
BstSFI CTRYAG 1 cut(s) 283
BstSLI GKGCMC 1 cut(s) 392
BstV1I GCAGC 2 cut(s) 161, 281
BstX2I RGATCY 1 cut(s) 157
BstYI RGATCY 1 cut(s) 157
Bsu15I ATCGAT 1 cut(s) 135
BsuRI GGCC 2 cut(s) 32, 319
BsuTUI ATCGAT 1 cut(s) 135
BtgI CCRYGG 1 cut(s) 120
BtrI CACGTC 1 cut(s) 545
BtsCI GGATG 2 cut(s) 256, 385
BtsI GCAGTG 1 cut(s) 271
BtsIMutI CAGTG 3 cut(s) 271, 392, 450
CaiI CAGNNNCTG 1 cut(s) 161
Cfr13I GGNCC 2 cut(s) 318, 424
ClaI ATCGAT 1 cut(s) 135
Csp6I GTAC 1 cut(s) 190
CviAII CATG 5 cut(s) 121, 178, 247, 354, 421
CviJI RGCY 7 cut(s) 32, 174, 272, 319, 452, 493, 530
CviKI_1 RGCY 7 cut(s) 32, 174, 272, 319, 452, 493, 530
CviQI GTAC 1 cut(s) 190
DdeI CTNAG 1 cut(s) 526
DpnI GATC 3 cut(s) 138, 159, 352
DpnII GATC 3 cut(s) 136, 157, 350
Eam1104I CTCTTC 2 cut(s) 104, 210
EarI CTCTTC 2 cut(s) 104, 210
Eco130I CCWWGG 2 cut(s) 33, 120
Eco147I AGGCCT 1 cut(s) 32
Eco47I GGWCC 1 cut(s) 424
Eco57I CTGAAG 2 cut(s) 85, 426
EcoO109I RGGNCCY 1 cut(s) 318
EcoT14I CCWWGG 2 cut(s) 33, 120
ErhI CCWWGG 2 cut(s) 33, 120
FaeI CATG 5 cut(s) 124, 181, 250, 357, 424
FatI CATG 5 cut(s) 120, 177, 246, 353, 420
FblI GTMKAC 1 cut(s) 541
Fnu4HI GCNGC 2 cut(s) 175, 270
FokI GGATG 2 cut(s) 243, 392
Fsp4HI GCNGC 2 cut(s) 175, 270
FspBI CTAG 1 cut(s) 195
GluI GCNGC 2 cut(s) 175, 270
HaeIII GGCC 2 cut(s) 32, 319
HapII CCGG 2 cut(s) 348, 427
Hin1II CATG 5 cut(s) 124, 181, 250, 357, 424
HincII GTYRAC 1 cut(s) 542
HindII GTYRAC 1 cut(s) 542
HinfI GANTC 5 cut(s) 71, 344, 374, 486, 535
HpaII CCGG 2 cut(s) 348, 427
Hpy166II GTNNAC 4 cut(s) 40, 149, 542, 565
Hpy188I TCNGA 3 cut(s) 157, 379, 579
Hpy188III TCNNGA 3 cut(s) 348, 364, 458
Hpy8I GTNNAC 4 cut(s) 40, 149, 542, 565
Hpy99I CGWCG 2 cut(s) 543, 546
HpyAV CCTTC 2 cut(s) 22, 450
HpyCH4III ACNGT 1 cut(s) 130
HpyCH4IV ACGT 1 cut(s) 544
HpyCH4V TGCA 4 cut(s) 177, 256, 269, 357
HpyF10VI GCNNNNNNNGC 1 cut(s) 490
HpyF3I CTNAG 1 cut(s) 526
HpySE526I ACGT 1 cut(s) 544
Hsp92II CATG 5 cut(s) 124, 181, 250, 357, 424
Kpn2I TCCGGA 1 cut(s) 347
Kzo9I GATC 3 cut(s) 136, 157, 350
LpnPI CCDG 8 cut(s) 60, 174, 271, 335, 361, 398, 440, 475
Lsp1109I GCAGC 2 cut(s) 161, 281
LweI GCATC 1 cut(s) 265
MaeI CTAG 1 cut(s) 195
MaeII ACGT 1 cut(s) 544
MaeIII GTNAC 2 cut(s) 4, 104
MalI GATC 3 cut(s) 138, 159, 352
MboI GATC 3 cut(s) 136, 157, 350
MboII GAAGA 2 cut(s) 91, 227
MflI RGATCY 1 cut(s) 157
MhlI GDGCHC 1 cut(s) 392
MluCI AATT 3 cut(s) 221, 433, 498
MlyI GAGTC 3 cut(s) 80, 480, 544
MnlI CCTC 6 cut(s) 211, 270, 288, 302, 402, 551
MroI TCCGGA 1 cut(s) 347
MseI TTAA 1 cut(s) 240
MspI CCGG 2 cut(s) 348, 427
MspR9I CCNGG 1 cut(s) 428
Mva1269I GAATGC 1 cut(s) 554
MwoI GCNNNNNNNGC 1 cut(s) 490
NciI CCSGG 1 cut(s) 428
NcoI CCATGG 1 cut(s) 120
NdeII GATC 3 cut(s) 136, 157, 350
NlaIII CATG 5 cut(s) 124, 181, 250, 357, 424
NlaIV GGNNCC 2 cut(s) 290, 320
NspI RCATGY 1 cut(s) 250
PceI AGGCCT 1 cut(s) 32
PctI GAATGC 1 cut(s) 554
PfeI GAWTC 2 cut(s) 344, 374
PkrI GCNGC 2 cut(s) 176, 271
PleI GAGTC 3 cut(s) 79, 480, 543
PpsI GAGTC 3 cut(s) 79, 480, 543
PsiI TTATAA 1 cut(s) 476
PspN4I GGNNCC 2 cut(s) 290, 320
PspPI GGNCC 2 cut(s) 318, 424
PstNI CAGNNNCTG 1 cut(s) 161
PsuI RGATCY 1 cut(s) 157
RsaI GTAC 1 cut(s) 191
RsaNI GTAC 1 cut(s) 190
SalI GTCGAC 1 cut(s) 540
SaqAI TTAA 1 cut(s) 240
SatI GCNGC 2 cut(s) 175, 270
Sau3AI GATC 3 cut(s) 136, 157, 350
Sau96I GGNCC 2 cut(s) 318, 424
SchI GAGTC 3 cut(s) 80, 480, 544
ScrFI CCNGG 1 cut(s) 428
SduI GDGCHC 1 cut(s) 392
SetI ASST 6 cut(s) 60, 111, 280, 294, 532, 547
SfaNI GCATC 1 cut(s) 265
SfcI CTRYAG 1 cut(s) 283
SgrDI CGTCGACG 1 cut(s) 540
SinI GGWCC 1 cut(s) 424
SmlI CTYRAG 2 cut(s) 364, 456
SmoI CTYRAG 2 cut(s) 364, 456
Sse9I AATT 3 cut(s) 221, 433, 498
SseBI AGGCCT 1 cut(s) 32
SspMI CTAG 1 cut(s) 195
StuI AGGCCT 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 426
StyI CCWWGG 2 cut(s) 33, 120
TaaI ACNGT 1 cut(s) 130
TaiI ACGT 1 cut(s) 547
TaqI TCGA 2 cut(s) 135, 541
TasI AATT 3 cut(s) 221, 433, 498
TfiI GAWTC 2 cut(s) 344, 374
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TscAI CASTG 3 cut(s) 271, 392, 450
TseI GCWGC 2 cut(s) 174, 269
TspDTI ATGAA 3 cut(s) 194, 296, 387
TspRI CASTG 3 cut(s) 271, 392, 450
VpaK11BI GGWCC 1 cut(s) 424
XapI RAATTY 1 cut(s) 221
XceI RCATGY 1 cut(s) 250
XmiI GTMKAC 1 cut(s) 541
XspI CTAG 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.