MD11G1083900.v1.1

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000148
Physical Location & Seq
Forward (+)
7090714 .. 7094228
3515 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1083900.v1.1.491

Sequence Viewer

Length: 1146 bp
ATGTTGTTACACCCTCGCAATGAAGCTGAGGATCTTTTGAGGAACCTGAAATTGAAATATGATCAATGTGAGCCCCCGCGGTACTTTAAGTGTTCTAATCGATATTGCCGCAGTTCTGCACAAAGGTTTAGTTATTACCAAAATGTTCATTGTTGTTGTGGGGGTCTGATGAACATGGAGACCATTTTTACAACGGAACAAGCTGAAGGTGGTGGGGTTTTTGTGGAAGGACCAGCGAGATTTATTATTAGTGATGATTTACAGGTACTGCCTCCATTTACTTGTGCAGTATCTTCTGTAGTTTCGAACCATGGACTCACAGGCTGGAAAAGCGTTGAGGAGCTGACTTTTAATGTGGGAGTTGATGAGGTTTTGAATTTGCTTATGCGCTCATTAGTATCAGAGACACCTCTGACTGAAACTCTGCTGAAGGATAAAACCATACCAAACATGGTTGACGAAAACCTTGACCAAGATTTATGCATTGAATATCGAGAGGTGGGAGGAAAAAGGAATGAGGAAGAAGAAAAGATTTCTATCAAACTTATAGTTAGCAAATCTAAGAAGAAGGTTTATTATGCCGAAGTGGGGGAGGATTTTGTTAATTTACTCTTCAGTTTCTTGACTCTTCCACTAGGGTTTGTAGTAAAACAAATGCAACATAACTCTTTGAAAGGTTGCATTGACCAGCTGTACAGAAGTGTCGAAGATCTTGATGAGCAGTACTTCAAGTCATATTTACACAAGACAAAGTTGGTGAGTCCGAAGCTTCTCCCTGGATTTGGCTACAAGAATCATCTTCTGGGAATTGAGGAAGCATCGTATAAATGGAAAGACTTTGTAAACTCCAAATCCCATCATAAAATTGATGATAAAGGTTGTGGATTCTTGAAAGGACCGGCAATGTTCATGGTAACTGACAGTCTCAATGTAAGTCCGATATCTGCAATTGTCGGCATGTCAATTCTATGCGAATTGAACGTACCTGTGACTGATGTTGAGGTACGAGTGGCTCATGTGGGGAAAGAGGAGGCTATCCGTCTTTTGGTGGCTTCTTTTGTTTGTCCCTCTGCTCTAACTAGTGTCTTCCTTACGAAGCCAAAGCGATTTGGAGATTTACTAGGGTGCCTAAGTTTTCTAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

382

Amino Acids

42.94

Weight (kDa)

6.04

Isoelectric Point (pI)

35.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 1 - 343 2e-85 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000148)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11145 AT3G09110 AT3G09120 AT3G09140 AT3G09140 AT5G01120 AT5G01130 AT5G01140 AT5G01150 AT5G37320 AT5G43240 AT5G43240 AT5G43240 AT5G43240
fragaria_vesca FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37580 FvH4_3g37580 FvH4_3g37590 FvH4_3g37590 FvH4_3g37590 FvH4_3g37601 FvH4_3g37610 FvH4_3g37610 FvH4_3g37640 FvH4_3g37640 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430
malus_domestica MD03G1079100.v1.1 MD03G1079200.v1.1 MD03G1079300.v1.1 MD03G1079500.v1.1 MD03G1079600.v1.1 MD03G1079700.v1.1 MD03G1079800.v1.1 MD11G1083300.v1.1 MD11G1083400.v1.1 MD11G1083500.v1.1 MD11G1083600.v1.1 MD11G1083700.v1.1 MD11G1083900.v1.1 MD11G1084000.v1.1 MD11G1084200.v1.1 MD11G1084300.v1.1
prunus_persica Prupe.1G077500_v2.0.a1 Prupe.6G063700_v2.0.a1 Prupe.6G063800_v2.0.a1 Prupe.6G063800_v2.0.a1 Prupe.6G063900_v2.0.a1 Prupe.6G064000_v2.0.a1 Prupe.6G064100_v2.0.a1 Prupe.6G064100_v2.0.a1 Prupe.6G064300_v2.0.a1 Prupe.6G064400_v2.0.a1
pyrus_communis pycom03g06310 pycom03g06320 pycom03g06330 pycom03g06340 pycom03g06350 pycom11g07140 pycom11g07160 pycom11g07170 pycom11g07190 pycom11g07200 pycom11g07230 pycom11g07240 pycom11g07250 pycom11g07260 pycom11g07270 pycom11g07280
rosa_chinensis RchiOBHm_Chr3g0490491 RchiOBHm_Chr5g0055481 RchiOBHm_Chr5g0067411 RchiOBHm_Chr5g0067441 RchiOBHm_Chr5g0067471 RchiOBHm_Chr5g0067491 RchiOBHm_Chr5g0067511 RchiOBHm_Chr5g0067521 RchiOBHm_Chr5g0067571 RchiOBHm_Chr5g0067581 RchiOBHm_Chr5g0067661 RchiOBHm_Chr5g0067671 RchiOBHm_Chr5g0067691 RchiOBHm_Chr5g0067721 RchiOBHm_Chr5g0067751 RchiOBHm_Chr5g0067801 RchiOBHm_Chr5g0067821 RchiOBHm_Chr5g0067831 RchiOBHm_Chr5g0067841 RchiOBHm_Chr5g0067861 RchiOBHm_Chr5g0067891 RchiOBHm_Chr5g0068351 RchiOBHm_Chr6g0278351
rosa_laevigata RLG00000013235 RLG00000022886 RLG00000035934 RLG00000035935 RLG00000035936 RLG00000035938 RLG00000035939 RLG00000035940 RLG00000035942 RLG00000035968
rosa_multiflora Rmu_sc0000230.1_g000001 Rmu_sc0000435.1_g000052 Rmu_sc0000435.1_g000053 Rmu_sc0000435.1_g000057 Rmu_sc0000435.1_g000067 Rmu_sc0000435.1_g000080 Rmu_sc0001016.1_g000006 Rmu_sc0001418.1_g000006 Rmu_sc0001418.1_g000008 Rmu_sc0002253.1_g000012 Rmu_sc0002253.1_g000013 Rmu_sc0002253.1_g000038 Rmu_sc0002253.1_g000039 Rmu_sc0002253.1_g000044 Rmu_sc0002489.1_g000055 Rmu_sc0003862.1_g000013 Rmu_sc0007131.1_g000003 Rmu_sc0007131.1_g000004 Rmu_sc0008054.1_g000001 Rmu_sc0009958.1_g000001 Rmu_sc0016063.1_g000001 Rmu_sc0022825.1_g000001
rosa_roxburghii Rroxscaffold_1G00012750 Rroxscaffold_1G00012810 Rroxscaffold_1G00012820 Rroxscaffold_1G00012860 Rroxscaffold_1G00012880 Rroxscaffold_1G00012920 Rroxscaffold_1G00012930 Rroxscaffold_1G00012940 Rroxscaffold_1G00013430 Rroxscaffold_1G00013440 Rroxscaffold_1G00013460 Rroxscaffold_1G00013510 Rroxscaffold_1G00013530 Rroxscaffold_1G00013550 Rroxscaffold_1G00013570 Rroxscaffold_1G00013580 Rroxscaffold_1G00013610 Rroxscaffold_1G00013620 Rroxscaffold_1G00013630 Rroxscaffold_1G00013680 Rroxscaffold_7G00190460
rosa_rugosa Rorug05G0192300 Rorug05G0192400 Rorug05G0192500 Rorug05G0192600 Rorug05G0192700 Rorug05G0384600 Rorug05G0385300 Rorug05G0385400 Rorug05G0385400 Rorug05G0385500 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385800 Rorug05G0386000 Rorug05G0389000 Rorug05G0389100 Rorug05G0397000 Rorug05G0397100
rosa_samantha Rh5BG461400 Rh5BG461600 Rh5BG461700 Rh5BG461900 Rh5BG462100 Rh6AG225700 Rh6AG225800
rosa_wichuraiana Rw0G017720 Rw3G025960 Rw5G041500 Rw5G041510 Rw5G041520 Rw5G041530 Rw5G041540 Rw5G041580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1125
AccII CGCG 1 cut(s) 79
AciI CCGC 3 cut(s) 77, 79, 109
AclWI GGATC 1 cut(s) 39
AcsI RAATTY 1 cut(s) 376
AcuI CTGAAG 3 cut(s) 225, 449, 598
AfaI GTAC 6 cut(s) 83, 267, 695, 725, 984, 1005
AfiI CCNNNNNNNGG 3 cut(s) 588, 782, 1045
AgsI TTSAA 7 cut(s) 55, 376, 488, 673, 730, 892, 979
AhlI ACTAGT 1 cut(s) 1079
AjnI CCWGG 1 cut(s) 775
AluBI AGCT 5 cut(s) 26, 203, 343, 691, 769
AluI AGCT 5 cut(s) 26, 203, 343, 691, 769
Alw26I GTCTC 3 cut(s) 173, 398, 929
AlwI GGATC 1 cut(s) 39
AlwNI CAGNNNCTG 1 cut(s) 268
ApoI RAATTY 1 cut(s) 376
AspLEI GCGC 1 cut(s) 390
AspS9I GGNCC 2 cut(s) 230, 896
AsuHPI GGTGA 1 cut(s) 769
AsuII TTCGAA 1 cut(s) 305
AvaII GGWCC 2 cut(s) 230, 896
BanI GGYRCC 1 cut(s) 1125
BanII GRGCYC 1 cut(s) 75
BbsI GAAGAC 1 cut(s) 1078
BbvCI CCTCAGC 1 cut(s) 27
BccI CCATC 1 cut(s) 864
BciT130I CCWGG 1 cut(s) 777
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 3 cut(s) 173, 398, 929
BcuI ACTAGT 1 cut(s) 1079
BfaI CTAG 3 cut(s) 635, 1080, 1121
BfmI CTRYAG 1 cut(s) 297
BglII AGATCT 1 cut(s) 709
BisI GCNGC 1 cut(s) 109
BlsI GCNGC 1 cut(s) 110
BmcAI AGTACT 1 cut(s) 725
Bme1390I CCNGG 1 cut(s) 777
Bme18I GGWCC 2 cut(s) 230, 896
BmgT120I GGNCC 2 cut(s) 230, 896
BmiI GGNNCC 2 cut(s) 44, 1127
BmrFI CCNGG 1 cut(s) 777
BmsI GCATC 1 cut(s) 827
BpiI GAAGAC 1 cut(s) 1078
Bpu10I CCTNAGC 1 cut(s) 27
Bpu14I TTCGAA 1 cut(s) 305
Bsa29I ATCGAT 1 cut(s) 100
BsaBI GATNNNNATC 1 cut(s) 536
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 3 cut(s) 77, 310, 775
Bsc4I CCNNNNNNNGG 3 cut(s) 588, 782, 1045
Bse118I RCCGGY 1 cut(s) 898
Bse3DI GCAATG 2 cut(s) 25, 909
Bse8I GATNNNNATC 1 cut(s) 536
BseBI CCWGG 1 cut(s) 777
BseCI ATCGAT 1 cut(s) 100
BseDI CCNNGG 3 cut(s) 77, 310, 775
BseJI GATNNNNATC 1 cut(s) 536
BseLI CCNNNNNNNGG 3 cut(s) 588, 782, 1045
BseMI GCAATG 2 cut(s) 25, 909
BseMII CTCAG 1 cut(s) 18
BseRI GAGGAG 2 cut(s) 353, 1043
BsgI GTGCAG 2 cut(s) 102, 306
Bsh1236I CGCG 1 cut(s) 79
BshNI GGYRCC 1 cut(s) 1125
BshVI ATCGAT 1 cut(s) 100
BsiSI CCGG 1 cut(s) 899
BslFI GGGAC 1 cut(s) 1050
BslI CCNNNNNNNGG 3 cut(s) 588, 782, 1045
BsmAI GTCTC 3 cut(s) 173, 398, 929
BsmFI GGGAC 1 cut(s) 1050
Bso31I GGTCTC 1 cut(s) 173
Bsp119I TTCGAA 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 75
Bsp1407I TGTACA 1 cut(s) 693
Bsp143I GATC 3 cut(s) 31, 61, 709
Bsp19I CCATGG 1 cut(s) 310
BspACI CCGC 3 cut(s) 77, 79, 109
BspCNI CTCAG 1 cut(s) 19
BspDI ATCGAT 1 cut(s) 100
BspFNI CGCG 1 cut(s) 79
BspLI GGNNCC 2 cut(s) 44, 1127
BspPI GGATC 1 cut(s) 39
BspT104I TTCGAA 1 cut(s) 305
BspT107I GGYRCC 1 cut(s) 1125
BspTNI GGTCTC 1 cut(s) 173
BsrDI GCAATG 2 cut(s) 25, 909
BsrFI RCCGGY 1 cut(s) 898
BsrGI TGTACA 1 cut(s) 693
BssAI RCCGGY 1 cut(s) 898
BssECI CCNNGG 3 cut(s) 77, 310, 775
BssMI GATC 3 cut(s) 31, 61, 709
BssT1I CCWWGG 1 cut(s) 310
Bst2UI CCWGG 1 cut(s) 777
Bst4CI ACNGT 1 cut(s) 923
Bst6I CTCTTC 2 cut(s) 617, 633
BstAUI TGTACA 1 cut(s) 693
BstBI TTCGAA 1 cut(s) 305
BstDEI CTNAG 3 cut(s) 27, 561, 1130
BstDSI CCRYGG 2 cut(s) 77, 310
BstFNI CGCG 1 cut(s) 79
BstHHI GCGC 1 cut(s) 390
BstKTI GATC 3 cut(s) 34, 64, 712
BstMAI GTCTC 3 cut(s) 173, 398, 929
BstMBI GATC 3 cut(s) 31, 61, 709
BstMWI GCNNNNNNNGC 1 cut(s) 330
BstNI CCWGG 1 cut(s) 777
BstNSI RCATGY 1 cut(s) 961
BstSCI CCNGG 1 cut(s) 775
BstSFI CTRYAG 1 cut(s) 297
BstUI CGCG 1 cut(s) 79
BstV2I GAAGAC 1 cut(s) 1078
BstX2I RGATCY 2 cut(s) 31, 709
BstYI RGATCY 2 cut(s) 31, 709
Bsu15I ATCGAT 1 cut(s) 100
BsuTUI ATCGAT 1 cut(s) 100
BtgI CCRYGG 2 cut(s) 77, 310
CaiI CAGNNNCTG 1 cut(s) 268
CfoI GCGC 1 cut(s) 390
Cfr10I RCCGGY 1 cut(s) 898
Cfr13I GGNCC 2 cut(s) 230, 896
Cfr42I CCGCGG 1 cut(s) 80
ClaI ATCGAT 1 cut(s) 100
Csp6I GTAC 6 cut(s) 82, 266, 694, 724, 983, 1004
CviAII CATG 6 cut(s) 175, 311, 451, 910, 958, 1016
CviQI GTAC 6 cut(s) 82, 266, 694, 724, 983, 1004
DdeI CTNAG 3 cut(s) 27, 561, 1130
DpnI GATC 3 cut(s) 33, 63, 711
DpnII GATC 3 cut(s) 31, 61, 709
Eam1104I CTCTTC 2 cut(s) 617, 633
EarI CTCTTC 2 cut(s) 617, 633
Eco130I CCWWGG 1 cut(s) 310
Eco24I GRGCYC 1 cut(s) 75
Eco31I GGTCTC 1 cut(s) 173
Eco32I GATATC 1 cut(s) 942
Eco47I GGWCC 2 cut(s) 230, 896
Eco57I CTGAAG 3 cut(s) 225, 449, 598
EcoRII CCWGG 1 cut(s) 775
EcoRV GATATC 1 cut(s) 942
EcoT14I CCWWGG 1 cut(s) 310
EcoT22I ATGCAT 1 cut(s) 485
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 310
FaeI CATG 6 cut(s) 178, 314, 454, 913, 961, 1019
FaqI GGGAC 1 cut(s) 1050
FatI CATG 6 cut(s) 174, 310, 450, 909, 957, 1015
FauI CCCGC 1 cut(s) 84
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 1 cut(s) 109
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 1 cut(s) 109
FspBI CTAG 3 cut(s) 635, 1080, 1121
GlaI GCGC 1 cut(s) 389
GluI GCNGC 1 cut(s) 109
HapII CCGG 1 cut(s) 899
HhaI GCGC 1 cut(s) 390
Hin1II CATG 6 cut(s) 178, 314, 454, 913, 961, 1019
Hin6I GCGC 1 cut(s) 388
HinP1I GCGC 1 cut(s) 388
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HindIII AAGCTT 1 cut(s) 767
HinfI GANTC 5 cut(s) 315, 625, 760, 793, 885
HpaII CCGG 1 cut(s) 899
HphI GGTGA 1 cut(s) 769
Hpy166II GTNNAC 2 cut(s) 457, 844
Hpy188I TCNGA 5 cut(s) 168, 403, 414, 765, 939
Hpy188III TCNNGA 4 cut(s) 494, 622, 713, 889
Hpy8I GTNNAC 2 cut(s) 457, 844
HpyAV CCTTC 4 cut(s) 200, 221, 424, 562
HpyCH4III ACNGT 1 cut(s) 923
HpyCH4IV ACGT 1 cut(s) 981
HpyCH4V TGCA 6 cut(s) 119, 287, 483, 658, 681, 947
HpyF10VI GCNNNNNNNGC 1 cut(s) 330
HpyF3I CTNAG 3 cut(s) 27, 561, 1130
HpySE526I ACGT 1 cut(s) 981
Hsp92II CATG 6 cut(s) 178, 314, 454, 913, 961, 1019
HspAI GCGC 1 cut(s) 388
Ksp22I TGATCA 1 cut(s) 61
KspI CCGCGG 1 cut(s) 80
Kzo9I GATC 3 cut(s) 31, 61, 709
LmnI GCTCC 1 cut(s) 340
LweI GCATC 1 cut(s) 827
MaeI CTAG 3 cut(s) 635, 1080, 1121
MaeII ACGT 1 cut(s) 981
MaeIII GTNAC 3 cut(s) 6, 913, 988
MalI GATC 3 cut(s) 33, 63, 711
MboI GATC 3 cut(s) 31, 61, 709
MboII GAAGA 9 cut(s) 285, 533, 536, 577, 604, 620, 719, 791, 1078
MfeI CAATTG 1 cut(s) 948
MflI RGATCY 2 cut(s) 31, 709
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 9 cut(s) 50, 376, 604, 807, 864, 948, 963, 974, 1140
MlyI GAGTC 3 cut(s) 309, 619, 769
Mph1103I ATGCAT 1 cut(s) 485
MseI TTAA 4 cut(s) 87, 351, 603, 1144
MspA1I CMGCKG 2 cut(s) 79, 691
MspI CCGG 1 cut(s) 899
MspR9I CCNGG 1 cut(s) 777
MunI CAATTG 1 cut(s) 948
MvaI CCWGG 1 cut(s) 777
MvnI CGCG 1 cut(s) 79
MwoI GCNNNNNNNGC 1 cut(s) 330
NcoI CCATGG 1 cut(s) 310
NdeII GATC 3 cut(s) 31, 61, 709
NlaIII CATG 6 cut(s) 178, 314, 454, 913, 961, 1019
NlaIV GGNNCC 2 cut(s) 44, 1127
NmuCI GTSAC 1 cut(s) 988
NsiI ATGCAT 1 cut(s) 485
NspI RCATGY 1 cut(s) 961
NspV TTCGAA 1 cut(s) 305
PfeI GAWTC 2 cut(s) 793, 885
PkrI GCNGC 1 cut(s) 110
PleI GAGTC 3 cut(s) 309, 619, 768
PpsI GAGTC 3 cut(s) 309, 619, 768
Psp6I CCWGG 1 cut(s) 775
PspGI CCWGG 1 cut(s) 775
PspN4I GGNNCC 2 cut(s) 44, 1127
PspPI GGNCC 2 cut(s) 230, 896
PstNI CAGNNNCTG 1 cut(s) 268
PsuI RGATCY 2 cut(s) 31, 709
PvuII CAGCTG 1 cut(s) 691
RsaI GTAC 6 cut(s) 83, 267, 695, 725, 984, 1005
RsaNI GTAC 6 cut(s) 82, 266, 694, 724, 983, 1004
SacII CCGCGG 1 cut(s) 80
SaqAI TTAA 4 cut(s) 87, 351, 603, 1144
SatI GCNGC 1 cut(s) 109
Sau3AI GATC 3 cut(s) 31, 61, 709
Sau96I GGNCC 2 cut(s) 230, 896
ScaI AGTACT 1 cut(s) 725
SchI GAGTC 3 cut(s) 309, 619, 769
ScrFI CCNGG 1 cut(s) 777
SduI GDGCHC 1 cut(s) 75
SfaNI GCATC 1 cut(s) 827
SfcI CTRYAG 1 cut(s) 297
Sfr303I CCGCGG 1 cut(s) 80
SfuI TTCGAA 1 cut(s) 305
SgrBI CCGCGG 1 cut(s) 80
SinI GGWCC 2 cut(s) 230, 896
SpeI ACTAGT 1 cut(s) 1079
Sse9I AATT 9 cut(s) 50, 376, 604, 807, 864, 948, 963, 974, 1140
SsiI CCGC 3 cut(s) 77, 79, 109
SspMI CTAG 3 cut(s) 635, 1080, 1121
StyD4I CCNGG 1 cut(s) 775
StyI CCWWGG 1 cut(s) 310
TaaI ACNGT 1 cut(s) 923
TaiI ACGT 1 cut(s) 984
TaqI TCGA 4 cut(s) 100, 305, 493, 705
TasI AATT 9 cut(s) 50, 376, 604, 807, 864, 948, 963, 974, 1140
TatI WGTACW 2 cut(s) 693, 723
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 2 cut(s) 793, 885
Tru1I TTAA 4 cut(s) 87, 351, 603, 1144
Tru9I TTAA 4 cut(s) 87, 351, 603, 1144
TseFI GTSAC 1 cut(s) 988
Tsp45I GTSAC 1 cut(s) 988
TspDTI ATGAA 4 cut(s) 36, 137, 185, 898
TspGWI ACGGA 2 cut(s) 209, 1028
VpaK11BI GGWCC 2 cut(s) 230, 896
XapI RAATTY 1 cut(s) 376
XceI RCATGY 1 cut(s) 961
XcmI CCANNNNNNNNNTGG 1 cut(s) 448
XspI CTAG 3 cut(s) 635, 1080, 1121
ZrmI AGTACT 1 cut(s) 725
Zsp2I ATGCAT 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.