MD03G1079600.v1.1

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000148
Physical Location & Seq
Reverse (-)
6409652 .. 6412848
3197 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1079600.v1.1.491

Sequence Viewer

Length: 1440 bp
ATGGCTGAAAAAAAGCGTGCGAAGAATCGCAAAAATGTAAGCTTGAAGGCCTTGATGGACACGGGAAGCAACAAAGTTATCTTCGTTGAGTCCAACAGTGATTTTATTGATGTTCTCTTCAGTTTCTTGACAATGCCAATGGGAAGGATTGCCAGGCTTTCCTGTACATCTTCAGTACCGGTGGAAATAGGTTGTATGAGAAATTTGTATCAGAGTATTGAGAAATTTGATGTGCATGACTTCCGGTCAAATGCGTGTAGAGATATGTTGTTACTTCCCTGCAATGCTGCTGATTGTCAATGCAAGAACCTCAAGTTGAAAATTGACAATGGTAGGCCGACACGGTACTTTTTGTGCTATGACAAATGCTTCTTAAGCTATTATGGAGATATCCCCTGTCCTTTCTGTACTTGTCCCTTGACTAGGGAGATGCATCTTCCGGTGGATAATACTCAAGCTGGAGGAGTCTTTGTGAAAGGGCCATCCAGGCTGATAATTACCGATGATTTACAGGTCATATCCCCAGTAAGTGCAGCAAGCATTTGTTTGTTTTCGAAGCTACGGGTCACGGATTCTAAAACTACAGAGGAGTCGACTTTCAATATGGGAGTTCAGGAGGTTTTGGATTTGCTTATGCATTCGTTTGTATCAAAGACACCTTTGACTGAAACTCTTCTGAAGAACAAACCAATACCGAATTTGGGAGACGTACATTCCAGTCAAGGAACATCGATTGATTCTCAAATGCATGGCGACACAATCATTGAGGAAGAGAAAAACATTTCTCTTAAGATTGTAGTTAGCAAGTCTAAAAAAATTGTTTGTTATGCAGAAGCACAAGAGGATTTTGTTAATCTACTCTGCAGTTTCTTGACTCTCCCACTTGGGCTTATATTAAAAAAGATGCGAAATGTTTCATGGAAAGGCTGTCTTGATCAGTTGTACAAGAGTGTCGAAGATCTTGATGAGCAATACTTGATGTCAAATTACCACAAGGAATTGTTGGTCAATCCAAAGCTTGCACCTGGTTATCGATATGAGAAAACTCTGCTAGGAATCGAGGAGACGTCCTTTTATTATGTTGACAAGAAGTTAACTACTGATACATCCTGTATTCCTGATAATAGTTTAGTGGAGTCAGTTAAACTTGATGTTGTTGATCCCAAATCTCATGAAGATAAAGATAGAAGTGCTCAAGGATTTCTGAGAGGACCTGCTATCTTCACAGTGACTGACAATCTAGTTGTAAGACCGATATCTCCAATGCTTGAAATCTCTGTTCTTCAAGAATTAAAGGTACCTTTCACTGACATCGAGGATCATACCGTGCATGTGGGCAAGAGAGAGGCGTTGCTTCTTTTGGCGGCCTCATTTATCGGTGATTCTGCTCTTACCAATACCTTCATTTCAGAGCTTAGGGAGCCAAAGCAAGAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

480

Amino Acids

53.84

Weight (kDa)

5.93

Isoelectric Point (pI)

48.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 11 - 447 1.3e-119 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000148)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11145 AT3G09110 AT3G09120 AT3G09140 AT3G09140 AT5G01120 AT5G01130 AT5G01140 AT5G01150 AT5G37320 AT5G43240 AT5G43240 AT5G43240 AT5G43240
fragaria_vesca FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37580 FvH4_3g37580 FvH4_3g37590 FvH4_3g37590 FvH4_3g37590 FvH4_3g37601 FvH4_3g37610 FvH4_3g37610 FvH4_3g37640 FvH4_3g37640 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430
malus_domestica MD03G1079100.v1.1 MD03G1079200.v1.1 MD03G1079300.v1.1 MD03G1079500.v1.1 MD03G1079600.v1.1 MD03G1079700.v1.1 MD03G1079800.v1.1 MD11G1083300.v1.1 MD11G1083400.v1.1 MD11G1083500.v1.1 MD11G1083600.v1.1 MD11G1083700.v1.1 MD11G1083900.v1.1 MD11G1084000.v1.1 MD11G1084200.v1.1 MD11G1084300.v1.1
prunus_persica Prupe.1G077500_v2.0.a1 Prupe.6G063700_v2.0.a1 Prupe.6G063800_v2.0.a1 Prupe.6G063800_v2.0.a1 Prupe.6G063900_v2.0.a1 Prupe.6G064000_v2.0.a1 Prupe.6G064100_v2.0.a1 Prupe.6G064100_v2.0.a1 Prupe.6G064300_v2.0.a1 Prupe.6G064400_v2.0.a1
pyrus_communis pycom03g06310 pycom03g06320 pycom03g06330 pycom03g06340 pycom03g06350 pycom11g07140 pycom11g07160 pycom11g07170 pycom11g07190 pycom11g07200 pycom11g07230 pycom11g07240 pycom11g07250 pycom11g07260 pycom11g07270 pycom11g07280
rosa_chinensis RchiOBHm_Chr3g0490491 RchiOBHm_Chr5g0055481 RchiOBHm_Chr5g0067411 RchiOBHm_Chr5g0067441 RchiOBHm_Chr5g0067471 RchiOBHm_Chr5g0067491 RchiOBHm_Chr5g0067511 RchiOBHm_Chr5g0067521 RchiOBHm_Chr5g0067571 RchiOBHm_Chr5g0067581 RchiOBHm_Chr5g0067661 RchiOBHm_Chr5g0067671 RchiOBHm_Chr5g0067691 RchiOBHm_Chr5g0067721 RchiOBHm_Chr5g0067751 RchiOBHm_Chr5g0067801 RchiOBHm_Chr5g0067821 RchiOBHm_Chr5g0067831 RchiOBHm_Chr5g0067841 RchiOBHm_Chr5g0067861 RchiOBHm_Chr5g0067891 RchiOBHm_Chr5g0068351 RchiOBHm_Chr6g0278351
rosa_laevigata RLG00000013235 RLG00000022886 RLG00000035934 RLG00000035935 RLG00000035936 RLG00000035938 RLG00000035939 RLG00000035940 RLG00000035942 RLG00000035968
rosa_multiflora Rmu_sc0000230.1_g000001 Rmu_sc0000435.1_g000052 Rmu_sc0000435.1_g000053 Rmu_sc0000435.1_g000057 Rmu_sc0000435.1_g000067 Rmu_sc0000435.1_g000080 Rmu_sc0001016.1_g000006 Rmu_sc0001418.1_g000006 Rmu_sc0001418.1_g000008 Rmu_sc0002253.1_g000012 Rmu_sc0002253.1_g000013 Rmu_sc0002253.1_g000038 Rmu_sc0002253.1_g000039 Rmu_sc0002253.1_g000044 Rmu_sc0002489.1_g000055 Rmu_sc0003862.1_g000013 Rmu_sc0007131.1_g000003 Rmu_sc0007131.1_g000004 Rmu_sc0008054.1_g000001 Rmu_sc0009958.1_g000001 Rmu_sc0016063.1_g000001 Rmu_sc0022825.1_g000001
rosa_roxburghii Rroxscaffold_1G00012750 Rroxscaffold_1G00012810 Rroxscaffold_1G00012820 Rroxscaffold_1G00012860 Rroxscaffold_1G00012880 Rroxscaffold_1G00012920 Rroxscaffold_1G00012930 Rroxscaffold_1G00012940 Rroxscaffold_1G00013430 Rroxscaffold_1G00013440 Rroxscaffold_1G00013460 Rroxscaffold_1G00013510 Rroxscaffold_1G00013530 Rroxscaffold_1G00013550 Rroxscaffold_1G00013570 Rroxscaffold_1G00013580 Rroxscaffold_1G00013610 Rroxscaffold_1G00013620 Rroxscaffold_1G00013630 Rroxscaffold_1G00013680 Rroxscaffold_7G00190460
rosa_rugosa Rorug05G0192300 Rorug05G0192400 Rorug05G0192500 Rorug05G0192600 Rorug05G0192700 Rorug05G0384600 Rorug05G0385300 Rorug05G0385400 Rorug05G0385400 Rorug05G0385500 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385800 Rorug05G0386000 Rorug05G0389000 Rorug05G0389100 Rorug05G0397000 Rorug05G0397100
rosa_samantha Rh5BG461400 Rh5BG461600 Rh5BG461700 Rh5BG461900 Rh5BG462100 Rh6AG225700 Rh6AG225800
rosa_wichuraiana Rw0G017720 Rw3G025960 Rw5G041500 Rw5G041510 Rw5G041520 Rw5G041530 Rw5G041540 Rw5G041580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1070
Acc36I ACCTGC 1 cut(s) 1222
Acc65I GGTACC 1 cut(s) 1297
AccB1I GGYRCC 1 cut(s) 1297
AccI GTMKAC 1 cut(s) 593
AciI CCGC 1 cut(s) 1364
AclWI GGATC 2 cut(s) 1154, 1326
AcsI RAATTY 3 cut(s) 202, 224, 697
AcuI CTGAAG 3 cut(s) 103, 156, 698
AcyI GRCGYC 1 cut(s) 1067
AfaI GTAC 7 cut(s) 166, 177, 347, 409, 711, 944, 1299
AfiI CCNNNNNNNGG 2 cut(s) 423, 701
AflII CTTAAG 2 cut(s) 373, 788
AgeI ACCGGT 1 cut(s) 178
AgsI TTSAA 5 cut(s) 46, 319, 601, 1271, 1286
AhdI GACNNNNNGTC 1 cut(s) 244
AjnI CCWGG 3 cut(s) 152, 485, 1024
AluBI AGCT 6 cut(s) 42, 378, 458, 559, 1018, 1414
AluI AGCT 6 cut(s) 42, 378, 458, 559, 1018, 1414
Alw21I GWGCWC 1 cut(s) 1195
Alw26I GTCTC 2 cut(s) 699, 1058
AlwI GGATC 2 cut(s) 1154, 1326
AlwNI CAGNNNCTG 1 cut(s) 1232
AoxI GGCC 4 cut(s) 48, 335, 479, 1365
ApeKI GCWGC 2 cut(s) 287, 533
ApoI RAATTY 3 cut(s) 202, 224, 697
AsiGI ACCGGT 1 cut(s) 178
Asp700I GAANNNNTTC 2 cut(s) 672, 913
Asp718I GGTACC 1 cut(s) 1297
AspS9I GGNCC 2 cut(s) 479, 1211
AsuHPI GGTGA 1 cut(s) 1391
AsuII TTCGAA 1 cut(s) 554
AvaII GGWCC 1 cut(s) 1211
BanI GGYRCC 1 cut(s) 1297
Bbv12I GWGCWC 1 cut(s) 1195
BbvI GCAGC 2 cut(s) 274, 545
BccI CCATC 2 cut(s) 49, 490
BciT130I CCWGG 3 cut(s) 154, 487, 1026
BclI TGATCA 1 cut(s) 934
BcoDI GTCTC 2 cut(s) 699, 1058
BfaI CTAG 3 cut(s) 423, 1052, 1241
BfmI CTRYAG 2 cut(s) 582, 862
BfrI CTTAAG 2 cut(s) 373, 788
BfuAI ACCTGC 1 cut(s) 1222
BglI GCCNNNNNGGC 1 cut(s) 487
BglII AGATCT 1 cut(s) 958
BisI GCNGC 3 cut(s) 288, 534, 1365
BlsI GCNGC 3 cut(s) 289, 535, 1366
Bme1390I CCNGG 3 cut(s) 154, 487, 1026
Bme18I GGWCC 1 cut(s) 1211
BmeRI GACNNNNNGTC 1 cut(s) 244
BmgT120I GGNCC 2 cut(s) 479, 1211
BmiI GGNNCC 2 cut(s) 1299, 1422
BmrFI CCNGG 3 cut(s) 154, 487, 1026
BmrI ACTGGG 1 cut(s) 518
BmsI GCATC 3 cut(s) 420, 442, 894
BmuI ACTGGG 1 cut(s) 518
BpmI CTGGAG 1 cut(s) 480
Bpu10I CCTNAGC 1 cut(s) 1415
Bpu14I TTCGAA 1 cut(s) 554
BpuEI CTTGAG 3 cut(s) 296, 438, 1179
Bsa29I ATCGAT 2 cut(s) 731, 1033
BsaHI GRCGYC 1 cut(s) 1067
BsaWI WCCGGW 3 cut(s) 178, 243, 439
BsaXI ACNNNNNCTCC 2 cut(s) 456, 486
Bsc4I CCNNNNNNNGG 2 cut(s) 423, 701
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 2 cut(s) 524, 717
Bse3DI GCAATG 1 cut(s) 289
BseBI CCWGG 3 cut(s) 154, 487, 1026
BseCI ATCGAT 2 cut(s) 731, 1033
BseGI GGATG 2 cut(s) 482, 1106
BseLI CCNNNNNNNGG 2 cut(s) 423, 701
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 1 cut(s) 1196
BseNI ACTGG 2 cut(s) 524, 717
BseRI GAGGAG 3 cut(s) 477, 602, 1076
BseXI GCAGC 2 cut(s) 274, 545
BsgI GTGCAG 1 cut(s) 552
BshFI GGCC 4 cut(s) 50, 337, 481, 1367
BshNI GGYRCC 1 cut(s) 1297
BshTI ACCGGT 1 cut(s) 178
BshVI ATCGAT 2 cut(s) 731, 1033
BsiHKAI GWGCWC 1 cut(s) 1195
BsiSI CCGG 3 cut(s) 179, 244, 440
BslFI GGGAC 1 cut(s) 399
BslI CCNNNNNNNGG 2 cut(s) 423, 701
BsmAI GTCTC 2 cut(s) 699, 1058
BsmBI CGTCTC 2 cut(s) 699, 1058
BsmFI GGGAC 1 cut(s) 399
BsmI GAATGC 1 cut(s) 637
BsnI GGCC 4 cut(s) 50, 337, 481, 1367
Bsp119I TTCGAA 1 cut(s) 554
Bsp1286I GDGCHC 1 cut(s) 1195
Bsp1407I TGTACA 2 cut(s) 164, 942
Bsp143I GATC 4 cut(s) 934, 958, 1159, 1318
BspACI CCGC 1 cut(s) 1364
BspANI GGCC 4 cut(s) 50, 337, 481, 1367
BspCNI CTCAG 1 cut(s) 1197
BspDI ATCGAT 2 cut(s) 731, 1033
BspHI TCATGA 1 cut(s) 1171
BspLI GGNNCC 2 cut(s) 1299, 1422
BspMAI CTGCAG 1 cut(s) 866
BspMI ACCTGC 1 cut(s) 1222
BspPI GGATC 2 cut(s) 1154, 1326
BspT104I TTCGAA 1 cut(s) 554
BspT107I GGYRCC 1 cut(s) 1297
BspTI CTTAAG 2 cut(s) 373, 788
BsrDI GCAATG 1 cut(s) 289
BsrFI RCCGGY 1 cut(s) 178
BsrGI TGTACA 2 cut(s) 164, 942
BsrI ACTGG 2 cut(s) 524, 717
BssAI RCCGGY 1 cut(s) 178
BssMI GATC 4 cut(s) 934, 958, 1159, 1318
BssNI GRCGYC 1 cut(s) 1067
Bst2UI CCWGG 3 cut(s) 154, 487, 1026
Bst4CI ACNGT 4 cut(s) 98, 345, 1228, 1327
Bst6I CTCTTC 3 cut(s) 122, 678, 765
BstACI GRCGYC 1 cut(s) 1067
BstAFI CTTAAG 2 cut(s) 373, 788
BstAUI TGTACA 2 cut(s) 164, 942
BstBI TTCGAA 1 cut(s) 554
BstC8I GCNNGC 3 cut(s) 18, 538, 1020
BstDEI CTNAG 2 cut(s) 1205, 1415
BstENI CCTNNNNNAGG 1 cut(s) 421
BstF5I GGATG 2 cut(s) 482, 1106
BstKTI GATC 4 cut(s) 937, 961, 1162, 1321
BstMAI GTCTC 2 cut(s) 699, 1058
BstMBI GATC 4 cut(s) 934, 958, 1159, 1318
BstMWI GCNNNNNNNGC 3 cut(s) 375, 487, 1420
BstNI CCWGG 3 cut(s) 154, 487, 1026
BstNSI RCATGY 1 cut(s) 1334
BstSCI CCNGG 3 cut(s) 152, 485, 1024
BstSFI CTRYAG 2 cut(s) 582, 862
BstV1I GCAGC 2 cut(s) 274, 545
BstX2I RGATCY 1 cut(s) 958
BstYI RGATCY 1 cut(s) 958
Bsu15I ATCGAT 2 cut(s) 731, 1033
BsuRI GGCC 4 cut(s) 50, 337, 481, 1367
BsuTUI ATCGAT 2 cut(s) 731, 1033
BtsCI GGATG 2 cut(s) 482, 1106
BtsIMutI CAGTG 3 cut(s) 103, 1233, 1305
BveI ACCTGC 1 cut(s) 1222
Cac8I GCNNGC 3 cut(s) 18, 538, 1020
CaiI CAGNNNCTG 1 cut(s) 1232
CciI TCATGA 1 cut(s) 1171
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 2 cut(s) 479, 1211
ClaI ATCGAT 2 cut(s) 731, 1033
CsiI ACCWGGT 1 cut(s) 1024
Csp6I GTAC 7 cut(s) 165, 176, 346, 408, 710, 943, 1298
CspAI ACCGGT 1 cut(s) 178
CviAII CATG 5 cut(s) 236, 749, 918, 1172, 1331
CviQI GTAC 7 cut(s) 165, 176, 346, 408, 710, 943, 1298
DdeI CTNAG 2 cut(s) 1205, 1415
DpnI GATC 4 cut(s) 936, 960, 1161, 1320
DpnII GATC 4 cut(s) 934, 958, 1159, 1318
DriI GACNNNNNGTC 1 cut(s) 244
Eam1104I CTCTTC 3 cut(s) 122, 678, 765
Eam1105I GACNNNNNGTC 1 cut(s) 244
EarI CTCTTC 3 cut(s) 122, 678, 765
Eco147I AGGCCT 1 cut(s) 50
Eco32I GATATC 2 cut(s) 391, 1257
Eco47I GGWCC 1 cut(s) 1211
Eco57I CTGAAG 3 cut(s) 103, 156, 698
EcoNI CCTNNNNNAGG 1 cut(s) 421
EcoO109I RGGNCCY 1 cut(s) 1211
EcoRII CCWGG 3 cut(s) 152, 485, 1024
EcoRV GATATC 2 cut(s) 391, 1257
EcoT22I ATGCAT 3 cut(s) 435, 639, 750
Esp3I CGTCTC 2 cut(s) 699, 1058
FaeI CATG 5 cut(s) 239, 752, 921, 1175, 1334
FalI AAGNNNNNCTT 2 cut(s) 915, 947
FaqI GGGAC 1 cut(s) 399
FatI CATG 5 cut(s) 235, 748, 917, 1171, 1330
FbaI TGATCA 1 cut(s) 934
FblI GTMKAC 1 cut(s) 593
Fnu4HI GCNGC 3 cut(s) 288, 534, 1365
FokI GGATG 2 cut(s) 469, 1093
Fsp4HI GCNGC 3 cut(s) 288, 534, 1365
FspBI CTAG 3 cut(s) 423, 1052, 1241
GluI GCNGC 3 cut(s) 288, 534, 1365
GsuI CTGGAG 1 cut(s) 480
HaeIII GGCC 4 cut(s) 50, 337, 481, 1367
HapII CCGG 3 cut(s) 179, 244, 440
Hin1I GRCGYC 1 cut(s) 1067
Hin1II CATG 5 cut(s) 239, 752, 921, 1175, 1334
HincII GTYRAC 3 cut(s) 594, 1084, 1095
HindII GTYRAC 3 cut(s) 594, 1084, 1095
HindIII AAGCTT 2 cut(s) 40, 1016
HpaI GTTAAC 1 cut(s) 1095
HpaII CCGG 3 cut(s) 179, 244, 440
HphI GGTGA 1 cut(s) 1391
Hpy166II GTNNAC 3 cut(s) 594, 1084, 1095
Hpy188I TCNGA 4 cut(s) 213, 678, 1206, 1411
Hpy188III TCNNGA 8 cut(s) 127, 614, 871, 932, 962, 1118, 1172, 1286
Hpy8I GTNNAC 3 cut(s) 594, 1084, 1095
HpyAV CCTTC 3 cut(s) 40, 138, 1411
HpyCH4III ACNGT 4 cut(s) 98, 345, 1228, 1327
HpyCH4IV ACGT 2 cut(s) 708, 1067
HpyF10VI GCNNNNNNNGC 3 cut(s) 375, 487, 1420
HpyF3I CTNAG 2 cut(s) 1205, 1415
HpySE526I ACGT 2 cut(s) 708, 1067
Hsp92I GRCGYC 1 cut(s) 1067
Hsp92II CATG 5 cut(s) 239, 752, 921, 1175, 1334
KpnI GGTACC 1 cut(s) 1301
Ksp22I TGATCA 1 cut(s) 934
KspAI GTTAAC 1 cut(s) 1095
Kzo9I GATC 4 cut(s) 934, 958, 1159, 1318
LmnI GCTCC 1 cut(s) 1420
Lsp1109I GCAGC 2 cut(s) 274, 545
LweI GCATC 3 cut(s) 420, 442, 894
MabI ACCWGGT 1 cut(s) 1024
MaeI CTAG 3 cut(s) 423, 1052, 1241
MaeII ACGT 2 cut(s) 708, 1067
MaeIII GTNAC 3 cut(s) 270, 565, 1228
MalI GATC 4 cut(s) 936, 960, 1161, 1320
MboI GATC 4 cut(s) 934, 958, 1159, 1318
MflI RGATCY 1 cut(s) 958
MhlI GDGCHC 1 cut(s) 1195
MluCI AATT 9 cut(s) 202, 224, 321, 495, 697, 816, 985, 998, 1289
MlyI GAGTC 5 cut(s) 98, 474, 599, 868, 1145
MmeI TCCRAC 1 cut(s) 117
Mph1103I ATGCAT 3 cut(s) 435, 639, 750
MroXI GAANNNNTTC 2 cut(s) 672, 913
MseI TTAA 7 cut(s) 374, 789, 852, 896, 1094, 1143, 1292
MspCI CTTAAG 2 cut(s) 373, 788
MspI CCGG 3 cut(s) 179, 244, 440
MspR9I CCNGG 3 cut(s) 154, 487, 1026
Mva1269I GAATGC 1 cut(s) 637
MvaI CCWGG 3 cut(s) 154, 487, 1026
MwoI GCNNNNNNNGC 3 cut(s) 375, 487, 1420
NdeII GATC 4 cut(s) 934, 958, 1159, 1318
NlaIII CATG 5 cut(s) 239, 752, 921, 1175, 1334
NlaIV GGNNCC 2 cut(s) 1299, 1422
NmuCI GTSAC 2 cut(s) 565, 1228
NsiI ATGCAT 3 cut(s) 435, 639, 750
NspI RCATGY 1 cut(s) 1334
NspV TTCGAA 1 cut(s) 554
PagI TCATGA 1 cut(s) 1171
PceI AGGCCT 1 cut(s) 50
PctI GAATGC 1 cut(s) 637
PdmI GAANNNNTTC 2 cut(s) 672, 913
PfeI GAWTC 5 cut(s) 25, 572, 737, 1056, 1382
PinAI ACCGGT 1 cut(s) 178
PkrI GCNGC 3 cut(s) 289, 535, 1366
PleI GAGTC 5 cut(s) 97, 473, 598, 868, 1144
PpsI GAGTC 5 cut(s) 97, 473, 598, 868, 1144
PpuMI RGGWCCY 1 cut(s) 1211
Psp5II RGGWCCY 1 cut(s) 1211
Psp6I CCWGG 3 cut(s) 152, 485, 1024
PspGI CCWGG 3 cut(s) 152, 485, 1024
PspN4I GGNNCC 2 cut(s) 1299, 1422
PspPI GGNCC 2 cut(s) 479, 1211
PspPPI RGGWCCY 1 cut(s) 1211
PstI CTGCAG 1 cut(s) 866
PstNI CAGNNNCTG 1 cut(s) 1232
PsuI RGATCY 1 cut(s) 958
RsaI GTAC 7 cut(s) 166, 177, 347, 409, 711, 944, 1299
RsaNI GTAC 7 cut(s) 165, 176, 346, 408, 710, 943, 1298
SalI GTCGAC 1 cut(s) 592
SaqAI TTAA 7 cut(s) 374, 789, 852, 896, 1094, 1143, 1292
SatI GCNGC 3 cut(s) 288, 534, 1365
Sau3AI GATC 4 cut(s) 934, 958, 1159, 1318
Sau96I GGNCC 2 cut(s) 479, 1211
SchI GAGTC 5 cut(s) 98, 474, 599, 868, 1145
ScrFI CCNGG 3 cut(s) 154, 487, 1026
SduI GDGCHC 1 cut(s) 1195
SexAI ACCWGGT 1 cut(s) 1024
SfaNI GCATC 3 cut(s) 420, 442, 894
SfcI CTRYAG 2 cut(s) 582, 862
SfuI TTCGAA 1 cut(s) 554
SinI GGWCC 1 cut(s) 1211
SmlI CTYRAG 5 cut(s) 311, 373, 453, 788, 1194
SmoI CTYRAG 5 cut(s) 311, 373, 453, 788, 1194
Sse9I AATT 9 cut(s) 202, 224, 321, 495, 697, 816, 985, 998, 1289
SseBI AGGCCT 1 cut(s) 50
SsiI CCGC 1 cut(s) 1364
SspMI CTAG 3 cut(s) 423, 1052, 1241
StuI AGGCCT 1 cut(s) 50
StyD4I CCNGG 3 cut(s) 152, 485, 1024
TaaI ACNGT 4 cut(s) 98, 345, 1228, 1327
TaiI ACGT 2 cut(s) 711, 1070
TaqI TCGA 7 cut(s) 554, 593, 731, 954, 1033, 1059, 1314
TaqII GACCGA 1 cut(s) 1267
TasI AATT 9 cut(s) 202, 224, 321, 495, 697, 816, 985, 998, 1289
TatI WGTACW 3 cut(s) 164, 407, 942
TauI GCSGC 1 cut(s) 1367
TfiI GAWTC 5 cut(s) 25, 572, 737, 1056, 1382
Tru1I TTAA 7 cut(s) 374, 789, 852, 896, 1094, 1143, 1292
Tru9I TTAA 7 cut(s) 374, 789, 852, 896, 1094, 1143, 1292
TscAI CASTG 3 cut(s) 103, 1233, 1312
TseFI GTSAC 2 cut(s) 565, 1228
TseI GCWGC 2 cut(s) 287, 533
Tsp45I GTSAC 2 cut(s) 565, 1228
TspDTI ATGAA 3 cut(s) 906, 1188, 1393
TspGWI ACGGA 1 cut(s) 584
TspRI CASTG 3 cut(s) 103, 1233, 1312
Vha464I CTTAAG 2 cut(s) 373, 788
VpaK11BI GGWCC 1 cut(s) 1211
XagI CCTNNNNNAGG 1 cut(s) 421
XapI RAATTY 3 cut(s) 202, 224, 697
XceI RCATGY 1 cut(s) 1334
XmiI GTMKAC 1 cut(s) 593
XmnI GAANNNNTTC 2 cut(s) 672, 913
XspI CTAG 3 cut(s) 423, 1052, 1241
ZraI GACGTC 1 cut(s) 1068
Zsp2I ATGCAT 3 cut(s) 435, 639, 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.